Restriction map for
pFN21AA0783
(Circular) (Six-base) MAPSORT of: pFN21AA0783.seq Check: 940 from: 1 to: 7369
pFN21AA0783 7369 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2020 2020
Size: 7369
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5179 278
Size: 53 83 186 936 3643 2468
Fragments arranged by size:
3643 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4712 1937
Size: 2775 4594
Acc65I G'GTAC_C
Cuts at: 4690 4690
Size: 7369
AclI AA'CG_TT
Cuts at: 1563 5496 5869 1563
Size: 3933 373 3063
Fragments arranged by size:
3933 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2142 2208 3611 3708 4617 4650 5432 6816
Size: 510 66 1403 97 909 33 782 1384
Cuts at: 6816 7084 1632
Size: 268 1917
Fragments arranged by size:
1917 1403 1384 909 782 510 268 97
66 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7172
AflIII A'CryG_T
Cuts at: 1203 6268 7245 1203
Size: 5065 977 1327
Fragments arranged by size:
5065 1327 977
AgeI A'CCGG_T
Cuts at: 6200 6200
Size: 7369
AhdI GACnn_n'nnGTC
Cuts at: 6098 6098
Size: 7369
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7369
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3406 3654 1184
Size: 2222 248 4899
Fragments arranged by size:
4899 2222 248
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3198 3230 3198
Size: 32 7337
AlwNI CAG_nnn'CTG
Cuts at: 2120 2426 3098 3203 5112 6684 2120
Size: 306 672 105 1909 1572 2805
Fragments arranged by size:
2805 1909 1572 672 306 105
ApaLI G'TGCA_C
Cuts at: 5426 6582 5426
Size: 1156 6213
ApoI r'AATT_y
Cuts at: 1493 2251 3183 3900 4430 4678 4904 5056 7165
Size: 758 932 717 530 248 226 152 2109
Cuts at: 7165 1493
Size: 1697
Fragments arranged by size:
2109 1697 932 758 717 530 248 226
152
AseI AT'TA_AT
Cuts at: 160 1032 2291 5923 160
Size: 872 1259 3632 1606
Fragments arranged by size:
3632 1606 1259 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7369
AvaI C'yCGr_G
Cuts at: 1870 1943 4694 1870
Size: 73 2751 4545
Fragments arranged by size:
4545 2751 73
BamHI G'GATC_C
Cuts at: 4270 4699 5066 4270
Size: 429 367 6573
Fragments arranged by size:
6573 429 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3314 4690 6145 618
Size: 325 217 627 1527 1376 1455 1842
Fragments arranged by size:
1842 1527 1455 1376 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2942 4688 1391
Size: 1551 1746 4072
Fragments arranged by size:
4072 1746 1551
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2754 3345 4564 928
Size: 971 855 591 1219 3733
Fragments arranged by size:
3733 1219 971 855 591
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5560 5594 1903
Size: 34 3623 34 3678
Fragments arranged by size:
3678 3623 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5261 6471 5261
Size: 1210 6159
BclI T'GATC_A
Cuts at: 1548 4210 1548
Size: 2662 4707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5980 136
Size: 107 122 71 631 605 4308 1525
Fragments arranged by size:
4308 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2675 7364 2675
Size: 4689 2680
BlpI GC'TnA_GC
Cuts at: 4786 4786
Size: 7369
Bme1580I G_kGCm'C
Cuts at: 1790 3232 5430 6586 1790
Size: 1442 2198 1156 2573
Fragments arranged by size:
2573 2198 1442 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7369
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3369 5100 6058
Size: 416 53 83 399 24 1952 1731 958
Cuts at: 6058 442
Size: 1753
Fragments arranged by size:
1952 1753 1731 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7369
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3094 3094
Size: 7369
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2956 3264 4656 6029 1239
Size: 1717 308 1392 1373 2579
Fragments arranged by size:
2579 1717 1392 1373 308
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2939 5494 6359 6657 2939
Size: 2555 865 298 3651
Fragments arranged by size:
3651 2555 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6032 882
Size: 617 4533 2219
Fragments arranged by size:
4533 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5160 493
Size: 711 3956 2702
Fragments arranged by size:
3956 2702 711
BsaBI GATnn'nnATC
Cuts at: 2680 2775 4215 7363 2680
Size: 95 1440 3148 2686
Fragments arranged by size:
3148 2686 1440 95
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5176 5558 6193 275
Size: 53 83 186 936 3643 382 635 1451
Fragments arranged by size:
3643 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5802 6200 6474 6621 7032 5802
Size: 398 274 147 411 6139
Fragments arranged by size:
6139 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2458 2488 1178
Size: 30 165 30 1055 30 6059
Fragments arranged by size:
6059 1055 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2038 4266 1186
Size: 852 2228 4289
Fragments arranged by size:
4289 2228 852
BseYI C'CCAG_C
Cuts at: 6572 7068 6572
Size: 496 6873
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3412 1163
Size: 573 1676 5120
Fragments arranged by size:
5120 1676 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4819 5580 6608 1540
Size: 275 186 2818 761 1028 2301
Fragments arranged by size:
2818 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2942 3421 4111 4325 5430 5515 6586 2942
Size: 479 690 214 1105 85 1071 3725
Fragments arranged by size:
3725 1105 1071 690 479 214 85
BsmI GAATG_Cn'
Cuts at: 4880 4973 4880
Size: 93 7276
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2942 3232 3421 4111 4325 4688 5430
Size: 399 1152 290 189 690 214 363 742
Cuts at: 5430 5515 6586 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1152 1071 742 690 399 363 290
214 189 85
BspEI T'CCGG_A
Cuts at: 7032 7032
Size: 7369
BspHI T'CATG_A
Cuts at: 5258 6988 5258
Size: 1730 5639
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2020 4724 844
Size: 1176 2704 3489
Fragments arranged by size:
3489 2704 1176
BsrBI CCG'CTC
Cuts at: 1394 1823 5256 1394
Size: 429 3433 3507
Fragments arranged by size:
3507 3433 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2773 5864 6038 65
Size: 1183 1525 3091 174 1396
Fragments arranged by size:
3091 1525 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4093 6013 6200 1700
Size: 110 2283 1920 187 2869
Fragments arranged by size:
2869 2283 1920 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7369
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7369
BssSI C'ACGA_G
Cuts at: 4483 5429 6441 4483
Size: 946 1012 5411
Fragments arranged by size:
5411 1012 946
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7369
BstBI TT'CG_AA
Cuts at: 4030 6173 4030
Size: 2143 5226
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7369
BstXI CCAn_nnnn'nTGG
Cuts at: 2550 2550
Size: 7369
BstYI r'GATC_y
Cuts at: 1972 2675 3469 4270 4699 5066 5451 5468 6920
Size: 703 794 801 429 367 385 17 1452
Cuts at: 6920 7006 7018 7364 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 801 794 703 429 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4518 4518
Size: 7369
BtgI C'CryG_G
Cuts at: 513 1065 2004 2592 4371 513
Size: 552 939 588 1779 3511
Fragments arranged by size:
3511 1779 939 588 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6630
Fragments arranged by size:
6630 683 56
BtsI GCAGTG_nn'
Cuts at: 3724 3849 4325 4354 4885 5679 5699 3724
Size: 125 476 29 531 794 20 5394
Fragments arranged by size:
5394 794 531 476 125 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7369
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7334
DraI TTT'AAA
Cuts at: 2098 4673 2098
Size: 2575 4794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6376 809
Size: 507 5060 1802
Fragments arranged by size:
5060 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4368 4816 5705
Size: 54 1425 50 275 18 2538 448 889
Cuts at: 5705 7334 8
Size: 1629 43
Fragments arranged by size:
2538 1629 1425 889 448 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4816 1537
Size: 275 3004 4090
Fragments arranged by size:
4090 3004 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2592 2813 2918 3450 3518 4170 5299 1353
Size: 1239 221 105 532 68 652 1129 3423
Fragments arranged by size:
3423 1239 1129 652 532 221 105 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3467 5956 6330 6476 387
Size: 1064 138 1878 2489 374 146 1280
Fragments arranged by size:
2489 1878 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2940 2940
Size: 7369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2208 2956 3264 3611 3708 4617
Size: 393 510 66 748 308 347 97 909
Cuts at: 4617 4650 4656 5432 6029 6816 7084 1239
Size: 33 6 776 597 787 268 1524
Fragments arranged by size:
1524 909 787 776 748 597 510 393
347 308 268 97 66 33 6
EcoRI G'AATT_C
Cuts at: 4678 5056 4678
Size: 378 6991
EcoRV GAT'ATC
Cuts at: 3778 3778
Size: 7369
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2261 3452 3980 2261
Size: 1191 528 5650
Fragments arranged by size:
5650 1191 528
FspI TGC'GCA
Cuts at: 3890 5875 3890
Size: 1985 5384
FspAI rTGC'GCAy
Cuts at: 3890 3890
Size: 7369
HaeII r_GCGC'y
Cuts at: 1678 6516 1678
Size: 4838 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2573 2605 2869
Size: 32 135 32 428 32 541 32 264
Cuts at: 2869 2901 3403 3435 4147 4179 6010 6042 6084
Size: 32 502 32 712 32 1831 32 42
Cuts at: 6084 6116 1373
Size: 32 2626
Fragments arranged by size:
2626 1831 712 541 502 428 264 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2485 4713 4959 669
Size: 988 281 547 2228 246 3079
Fragments arranged by size:
3079 2228 988 547 281 246
HindIII A'AGCT_T
Cuts at: 748 3962 4507 748
Size: 3214 545 3610
Fragments arranged by size:
3610 3214 545
HpaI GTT'AAC
Cuts at: 2485 4959 2485
Size: 2474 4895
KpnI G_GTAC'C
Cuts at: 4694 4694
Size: 7369
MfeI C'AATT_G
Cuts at: 3025 4968 3025
Size: 1943 5426
MluI A'CGCG_T
Cuts at: 7245 7245
Size: 7369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2083 6483 6667 1305
Size: 778 4400 184 2007
Fragments arranged by size:
4400 2007 778 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4370 10
Size: 54 1425 343 2538 3009
Fragments arranged by size:
3009 2538 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5327 5686 5845 518
Size: 648 4161 359 159 2042
Fragments arranged by size:
4161 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3449 4785 5462 6610 6855 1606
Size: 1843 1336 677 1148 245 2120
Fragments arranged by size:
2120 1843 1336 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2592 4371 513
Size: 552 939 588 1779 3511
Fragments arranged by size:
3511 1779 939 588 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7369
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7369
NotI GC'GGCC_GC
Cuts at: 4816 4816
Size: 7369
NspI r_CATG'y
Cuts at: 1132 1229 3500 4727 6272 1132
Size: 97 2271 1227 1545 2229
Fragments arranged by size:
2271 2229 1545 1227 97
PacI TTA_AT'TAA
Cuts at: 2295 2295
Size: 7369
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7369
PciI A'CATG_T
Cuts at: 6268 6268
Size: 7369
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7369
PfoI T'CCnGG_A
Cuts at: 3241 3241
Size: 7369
PmeI GTTT'AAAC
Cuts at: 4673 4673
Size: 7369
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5438 5470 6951 6983 5438
Size: 32 1481 32 5824
Fragments arranged by size:
5824 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7369
PsiI TTA'TAA
Cuts at: 4939 4939
Size: 7369
PstI C_TGCA'G
Cuts at: 830 2260 3856 4174 4721 830
Size: 1430 1596 318 547 3478
Fragments arranged by size:
3478 1596 1430 547 318
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7369
SacI G_AGCT'C
Cuts at: 2942 2942
Size: 7369
SalI G'TCGA_C
Cuts at: 1936 4711 1936
Size: 2775 4594
SapI GCTCTTCn'nnn_
Cuts at: 2592 2592
Size: 7369
SbfI CC_TGCA'GG
Cuts at: 4721 4721
Size: 7369
ScaI AGT'ACT
Cuts at: 2271 4176 5617 7329 2271
Size: 1905 1441 1712 2311
Fragments arranged by size:
2311 1905 1712 1441
SfcI C'TryA_G
Cuts at: 826 1045 2176 2256 2520 2655 3545 3852 4170
Size: 219 1131 80 264 135 890 307 318
Cuts at: 4170 4717 5852 6533 6724 826
Size: 547 1135 681 191 1471
Fragments arranged by size:
1471 1135 1131 890 681 547 318 307
264 219 191 135 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7369
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7369
SmaI CCC'GGG
Cuts at: 1872 4696 1872
Size: 2824 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2918 5473 6374 6636 820
Size: 197 926 975 2555 901 262 1553
Fragments arranged by size:
2555 1553 975 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5160 493
Size: 4667 2702
SpeI A'CTAG_T
Cuts at: 152 3803 152
Size: 3651 3718
SphI G_CATG'C
Cuts at: 1132 4727 1132
Size: 3595 3774
SspI AAT'ATT
Cuts at: 5 52 2197 5293 5
Size: 47 2145 3096 2081
Fragments arranged by size:
3096 2145 2081 47
StuI AGG'CCT
Cuts at: 1517 3677 1517
Size: 2160 5209
StyI C'CwwG_G
Cuts at: 513 1065 2004 2592 3116 3318 3686 4113 4371
Size: 552 939 588 524 202 368 427 258
Cuts at: 4371 4590 4808 513
Size: 219 218 3074
Fragments arranged by size:
3074 939 588 552 524 427 368 258
219 218 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4126 5396 5413 5566 5751 6196 325
Size: 1255 2546 1270 17 153 185 445 1498
Fragments arranged by size:
2546 1498 1270 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2269 3770 4174
Size: 274 80 33 51 1443 292 1501 404
Cuts at: 4174 5615 7327 96
Size: 1441 1712 138
Fragments arranged by size:
1712 1501 1443 1441 404 292 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2458 2490 7329 7361 2458
Size: 32 4839 32 2466
Fragments arranged by size:
4839 2466 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7369
XbaI T'CTAG_A
Cuts at: 4705 4705
Size: 7369
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7369
XmaI C'CCGG_G
Cuts at: 1870 4694 1870
Size: 2824 4545
XmnI GAAnn'nnTTC
Cuts at: 3012 3963 4542 5498 3012
Size: 951 579 956 4883
Fragments arranged by size:
4883 956 951 579
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5177 276
Size: 53 83 186 936 3643 2468
Fragments arranged by size:
3643 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I FseI
KasI NaeI NarI NgoMIV NruI NsiI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI