Restriction map for
pFN21AA0772
(Circular) (Six-base) MAPSORT of: pFN21AA0772.seq Check: 3190 from: 1 to: 6109
pFN21AA0772 6109 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2684 2684
Size: 6109
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3919 278
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3452 1937
Size: 1515 4594
Acc65I G'GTAC_C
Cuts at: 3430 3430
Size: 6109
AclI AA'CG_TT
Cuts at: 1563 4236 4609 1563
Size: 2673 373 3063
Fragments arranged by size:
3063 2673 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2173 2228 4172 5556 5824 1632
Size: 541 55 1944 1384 268 1917
Fragments arranged by size:
1944 1917 1384 541 268 55
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5912
AflIII A'CryG_T
Cuts at: 1203 2307 5008 5985 1203
Size: 1104 2701 977 1327
Fragments arranged by size:
2701 1327 1104 977
AgeI A'CCGG_T
Cuts at: 4940 4940
Size: 6109
AhdI GACnn_n'nnGTC
Cuts at: 4838 4838
Size: 6109
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3068 1184
Size: 1884 4225
AlwNI CAG_nnn'CTG
Cuts at: 2347 3852 5424 2347
Size: 1505 1572 3032
Fragments arranged by size:
3032 1572 1505
ApaLI G'TGCA_C
Cuts at: 4166 5322 4166
Size: 1156 4953
ApoI r'AATT_y
Cuts at: 1493 2021 2136 3382 3418 3644 3796 5905 1493
Size: 528 115 1246 36 226 152 2109 1697
Fragments arranged by size:
2109 1697 1246 528 226 152 115 36
AseI AT'TA_AT
Cuts at: 160 1032 2453 4663 160
Size: 872 1421 2210 1606
Fragments arranged by size:
2210 1606 1421 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
AvaI C'yCGr_G
Cuts at: 1870 1943 2613 3434 1870
Size: 73 670 821 4545
Fragments arranged by size:
4545 821 670 73
BamHI G'GATC_C
Cuts at: 3439 3806 3439
Size: 367 5742
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2618 3430 4885 618
Size: 325 217 627 831 812 1455 1842
Fragments arranged by size:
1842 1455 831 812 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2613 3428 1391
Size: 1222 815 4072
Fragments arranged by size:
4072 1222 815
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2976 3158 928
Size: 971 1077 182 3879
Fragments arranged by size:
3879 1077 971 182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4300 4334 1903
Size: 34 2363 34 3678
Fragments arranged by size:
3678 2363 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4001 5211 4001
Size: 1210 4899
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4720 136
Size: 107 122 71 631 605 3048 1525
Fragments arranged by size:
3048 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6104 6104
Size: 6109
BlpI GC'TnA_GC
Cuts at: 3526 3526
Size: 6109
Bme1580I G_kGCm'C
Cuts at: 1790 4170 5326 1790
Size: 2380 1156 2573
Fragments arranged by size:
2573 2380 1156
BmgBI CAC'GTC
Cuts at: 1318 2995 3007 1318
Size: 1677 12 4420
Fragments arranged by size:
4420 1677 12
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2118 2392 3348
Size: 416 53 83 399 24 701 274 956
Cuts at: 3348 3840 4798 442
Size: 492 958 1753
Fragments arranged by size:
1753 958 956 701 492 416 399 274
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2139 2137
Size: 2 6107
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2369 2651 3079 3155 3266 4769 1239
Size: 1007 123 282 428 76 111 1503 2579
Fragments arranged by size:
2579 1503 1007 428 282 123 111 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2109 2564 4234 5099 5397 2109
Size: 455 1670 865 298 2821
Fragments arranged by size:
2821 1670 865 455 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2540 3017 4772 882
Size: 617 1041 477 1755 2219
Fragments arranged by size:
2219 1755 1041 617 477
BsaAI yAC'GTr
Cuts at: 493 1204 2308 3900 493
Size: 711 1104 1592 2702
Fragments arranged by size:
2702 1592 1104 711
BsaBI GATnn'nnATC
Cuts at: 2249 2482 6103 2249
Size: 233 3621 2255
Fragments arranged by size:
3621 2255 233
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3916 4298 4933 275
Size: 53 83 186 936 2383 382 635 1451
Fragments arranged by size:
2383 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4542 4940 5214 5361 5772 4542
Size: 398 274 147 411 4879
Fragments arranged by size:
4879 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2294 2312 2324 2342 3281
Size: 30 165 30 891 18 12 18 939
Cuts at: 3281 3311 1178
Size: 30 3976
Fragments arranged by size:
3976 939 891 165 30 30 30 18
18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2947 3263 3302 1186
Size: 1761 316 39 3993
Fragments arranged by size:
3993 1761 316 39
BseYI C'CCAG_C
Cuts at: 2340 5312 5808 2340
Size: 2972 496 2641
Fragments arranged by size:
2972 2641 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3050 1163
Size: 573 1314 4222
Fragments arranged by size:
4222 1314 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3559 4320 5348 1540
Size: 275 186 1558 761 1028 2301
Fragments arranged by size:
2301 1558 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2307 4170 4255 5326 2307
Size: 1863 85 1071 3090
Fragments arranged by size:
3090 1863 1071 85
BsmI GAATG_Cn'
Cuts at: 3620 3713 3620
Size: 93 6016
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2307 2613 3428 4170 4255 5326 1391
Size: 399 517 306 815 742 85 1071 2174
Fragments arranged by size:
2174 1071 815 742 517 399 306 85
BspEI T'CCGG_A
Cuts at: 5772 5772
Size: 6109
BspHI T'CATG_A
Cuts at: 3998 5728 3998
Size: 1730 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2684 3022 3464 844
Size: 1840 338 442 3489
Fragments arranged by size:
3489 1840 442 338
BsrBI CCG'CTC
Cuts at: 1394 1823 3241 3277 3379 3996 1394
Size: 429 1418 36 102 617 3507
Fragments arranged by size:
3507 1418 617 429 102 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 4604 4778 65
Size: 1183 3356 174 1396
Fragments arranged by size:
3356 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2161 2411 4753 4940 1700
Size: 110 351 250 2342 187 2869
Fragments arranged by size:
2869 2342 351 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6109
BssSI C'ACGA_G
Cuts at: 4169 5181 4169
Size: 1012 5097
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6109
BstBI TT'CG_AA
Cuts at: 4913 4913
Size: 6109
BstEII G'GTnAC_C
Cuts at: 1184 2939 1184
Size: 1755 4354
BstXI CCAn_nnnn'nTGG
Cuts at: 2629 2753 2629
Size: 124 5985
BstYI r'GATC_y
Cuts at: 1972 2896 3217 3439 3806 4191 4208 5660 5746
Size: 924 321 222 367 385 17 1452 86
Cuts at: 5746 5758 6104 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 924 385 367 346 321 222
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2557 3165 3193 513
Size: 552 1492 608 28 3429
Fragments arranged by size:
3429 1492 608 552 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2139 487
Size: 683 56 913 4457
Fragments arranged by size:
4457 913 683 56
BtsI GCAGTG_nn'
Cuts at: 3625 4419 4439 3625
Size: 794 20 5295
Fragments arranged by size:
5295 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6074
DraI TTT'AAA
Cuts at: 2742 2779 3413 2742
Size: 37 634 5438
Fragments arranged by size:
5438 634 37
DraIII CAC_nnn'GTG
Cuts at: 2815 2815
Size: 6109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5116 809
Size: 507 3800 1802
Fragments arranged by size:
3800 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3222 3556 4445
Size: 54 1425 50 275 18 1392 334 889
Cuts at: 4445 6074 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1392 889 334 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3556 1537
Size: 275 1744 4090
Fragments arranged by size:
4090 1744 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4039 1353
Size: 2686 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2621 4696 5070 5216 387
Size: 1064 138 1032 2075 374 146 1280
Fragments arranged by size:
2075 1280 1064 1032 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 2228 2246 2369 2651 3079 3155
Size: 393 541 55 18 123 282 428 76
Cuts at: 3155 3266 4172 4769 5556 5824 1239
Size: 111 906 597 787 268 1524
Fragments arranged by size:
1524 906 787 597 541 428 393 282
268 123 111 76 55 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2951 2951
Size: 6109
EcoO109I rG'GnC_Cy
Cuts at: 2118 2325 3342 2118
Size: 207 1017 4885
Fragments arranged by size:
4885 1017 207
EcoRI G'AATT_C
Cuts at: 2021 2136 3418 3796 2021
Size: 115 1282 378 4334
Fragments arranged by size:
4334 1282 378 115
EcoRV GAT'ATC
Cuts at: 3403 3403
Size: 6109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 6109
FspI TGC'GCA
Cuts at: 4615 4615
Size: 6109
HaeII r_GCGC'y
Cuts at: 1678 5256 1678
Size: 3578 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2607 2639 2957 2989 4750
Size: 32 135 32 1035 32 318 32 1761
Cuts at: 4750 4782 4824 4856 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1761 1035 318 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3453 3699 669
Size: 988 281 1515 246 3079
Fragments arranged by size:
3079 1515 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6109
HpaI GTT'AAC
Cuts at: 3699 3699
Size: 6109
KpnI G_GTAC'C
Cuts at: 3434 3434
Size: 6109
MfeI C'AATT_G
Cuts at: 3708 3708
Size: 6109
MluI A'CGCG_T
Cuts at: 5985 5985
Size: 6109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3414 5223 5407 1305
Size: 2109 1809 184 2007
Fragments arranged by size:
2109 2007 1809 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3224 10
Size: 54 1425 343 1392 2895
Fragments arranged by size:
2895 1425 1392 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2063 2763 4067 4426 4585 518
Size: 648 897 700 1304 359 159 2042
Fragments arranged by size:
2042 1304 897 700 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2284 3195 3525 4202 5350 5595 1606
Size: 678 911 330 677 1148 245 2120
Fragments arranged by size:
2120 1148 911 678 677 330 245
NaeI GCC'GGC
Cuts at: 2163 2163
Size: 6109
NcoI C'CATG_G
Cuts at: 513 1065 2557 513
Size: 552 1492 4065
Fragments arranged by size:
4065 1492 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6109
NgoMIV G'CCGG_C
Cuts at: 2161 2161
Size: 6109
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6109
NotI GC'GGCC_GC
Cuts at: 3556 3556
Size: 6109
NspI r_CATG'y
Cuts at: 1132 1229 3467 5012 1132
Size: 97 2238 1545 2229
Fragments arranged by size:
2238 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6109
PciI A'CATG_T
Cuts at: 5008 5008
Size: 6109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2347 1108
Size: 1239 4870
PfoI T'CCnGG_A
Cuts at: 2975 2975
Size: 6109
PmeI GTTT'AAAC
Cuts at: 3413 3413
Size: 6109
PmlI CAC'GTG
Cuts at: 2308 2308
Size: 6109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 5691 5723 4178
Size: 32 1481 32 4564
Fragments arranged by size:
4564 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3342 3342
Size: 6109
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6109
PsiI TTA'TAA
Cuts at: 3679 3679
Size: 6109
PstI C_TGCA'G
Cuts at: 830 3461 830
Size: 2631 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6109
SacII CC_GC'GG
Cuts at: 3196 3196
Size: 6109
SalI G'TCGA_C
Cuts at: 1936 3451 1936
Size: 1515 4594
SanDI GG'GwC_CC
Cuts at: 3342 3342
Size: 6109
SbfI CC_TGCA'GG
Cuts at: 3461 3461
Size: 6109
ScaI AGT'ACT
Cuts at: 4357 6069 4357
Size: 1712 4397
SfcI C'TryA_G
Cuts at: 826 1045 3457 4592 5273 5464 826
Size: 219 2412 1135 681 191 1471
Fragments arranged by size:
2412 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6109
SmaI CCC'GGG
Cuts at: 1872 3436 1872
Size: 1564 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2124 2579 4213 5114 5376 820
Size: 197 926 181 455 1634 901 262 1553
Fragments arranged by size:
1634 1553 926 901 455 262 197 181
SnaBI TAC'GTA
Cuts at: 493 3900 493
Size: 3407 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6109
SphI G_CATG'C
Cuts at: 1132 3467 1132
Size: 2335 3774
SspI AAT'ATT
Cuts at: 5 52 4033 5
Size: 47 3981 2081
Fragments arranged by size:
3981 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6109
StyI C'CwwG_G
Cuts at: 513 1065 2557 3282 3548 513
Size: 552 1492 725 266 3074
Fragments arranged by size:
3074 1492 725 552 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3178 4136 4153 4306 4491 4936 325
Size: 1255 1598 958 17 153 185 445 1498
Fragments arranged by size:
1598 1498 1255 958 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2295 4355 6067
Size: 274 80 33 51 1443 318 2060 1712
Cuts at: 6067 96
Size: 138
Fragments arranged by size:
2060 1712 1443 318 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2312 2344 2870 2902 3281 3313 6069 6101 2312
Size: 32 526 32 379 32 2756 32 2320
Fragments arranged by size:
2756 2320 526 379 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6109
XbaI T'CTAG_A
Cuts at: 3445 3445
Size: 6109
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6109
XmaI C'CCGG_G
Cuts at: 1870 3434 1870
Size: 1564 4545
XmnI GAAnn'nnTTC
Cuts at: 4238 4238
Size: 6109
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3917 276
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI FseI
FspAI KasI NarI NruI NsiI PacI PspOMI PspXI
PsrI PvuII RsrII SacI SapI SexAI SfiI SfoI
SrfI SwaI XcmI