Restriction map for
pFN21AA0765
(Circular) (Six-base) MAPSORT of: pFN21AA0765.seq Check: 6426 from: 1 to: 7501
pFN21AA0765 7501 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5311 278
Size: 53 83 186 936 3775 2468
Fragments arranged by size:
3775 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4844 1937
Size: 2907 4594
Acc65I G'GTAC_C
Cuts at: 4822 4822
Size: 7501
AclI AA'CG_TT
Cuts at: 1563 2513 5628 6001 1563
Size: 950 3115 373 3063
Fragments arranged by size:
3115 3063 950 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3559 3671 4254 5564 6948 7216 1632
Size: 1927 112 583 1310 1384 268 1917
Fragments arranged by size:
1927 1917 1384 1310 583 268 112
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7304
AflIII A'CryG_T
Cuts at: 1203 3337 3727 6400 7377 1203
Size: 2134 390 2673 977 1327
Fragments arranged by size:
2673 2134 1327 977 390
AgeI A'CCGG_T
Cuts at: 6332 6332
Size: 7501
AhdI GACnn_n'nnGTC
Cuts at: 6230 6230
Size: 7501
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7501
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4049 1184
Size: 2865 4636
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2464 2496 4293 4325 2464
Size: 32 1797 32 5640
Fragments arranged by size:
5640 1797 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4742 5244 6816 4742
Size: 502 1572 5427
Fragments arranged by size:
5427 1572 502
ApaI G_GGCC'C
Cuts at: 4370 4370
Size: 7501
ApaLI G'TGCA_C
Cuts at: 5558 6714 5558
Size: 1156 6345
ApoI r'AATT_y
Cuts at: 1493 2803 4375 4810 5036 5188 7297 1493
Size: 1310 1572 435 226 152 2109 1697
Fragments arranged by size:
2109 1697 1572 1310 435 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6055 160
Size: 872 5023 1606
Fragments arranged by size:
5023 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7501
AvaI C'yCGr_G
Cuts at: 1870 1943 4411 4722 4826 1870
Size: 73 2468 311 104 4545
Fragments arranged by size:
4545 2468 311 104 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2355 2388 2355
Size: 33 7468
BamHI G'GATC_C
Cuts at: 4831 5198 4831
Size: 367 7134
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2761 4822 6277 618
Size: 325 217 627 974 2061 1455 1842
Fragments arranged by size:
2061 1842 1455 974 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2510 2985 4370 4820 1391
Size: 1119 475 1385 450 4072
Fragments arranged by size:
4072 1385 1119 475 450
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6530
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5692 5726 1903
Size: 34 3755 34 3678
Fragments arranged by size:
3755 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2383 5393 6603 2383
Size: 3010 1210 3281
Fragments arranged by size:
3281 3010 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7501
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3250 4709 6112
Size: 107 122 71 631 605 1578 1459 1403
Cuts at: 6112 136
Size: 1525
Fragments arranged by size:
1578 1525 1459 1403 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7496 7496
Size: 7501
BlpI GC'TnA_GC
Cuts at: 4918 4918
Size: 7501
Bme1580I G_kGCm'C
Cuts at: 1790 2676 3643 4370 4450 5562 6718 1790
Size: 886 967 727 80 1112 1156 2573
Fragments arranged by size:
2573 1156 1112 967 886 727 80
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7501
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2132 2391 2681
Size: 416 53 83 399 24 715 259 290
Cuts at: 2681 3240 3701 3987 4077 5232 6190 442
Size: 559 461 286 90 1155 958 1753
Fragments arranged by size:
1753 1155 958 715 559 461 416 399
290 286 259 90 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2625 2811 3180 4206 6161 1239
Size: 1386 186 369 1026 1955 2579
Fragments arranged by size:
2579 1955 1386 1026 369 186
Bpu10I CC'TnA_GC
Cuts at: 2335 4399 2335
Size: 2064 5437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3048 5626 6491 6789 3048
Size: 2578 865 298 3760
Fragments arranged by size:
3760 2578 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2036 6164 882
Size: 617 537 4128 2219
Fragments arranged by size:
4128 2219 617 537
BsaAI yAC'GTr
Cuts at: 493 1204 3800 5292 493
Size: 711 2596 1492 2702
Fragments arranged by size:
2702 2596 1492 711
BsaBI GATnn'nnATC
Cuts at: 7495 7495
Size: 7501
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5308 5690 6325 275
Size: 53 83 186 936 3775 382 635 1451
Fragments arranged by size:
3775 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5934 6332 6606 6753 7164 5934
Size: 398 274 147 411 6271
Fragments arranged by size:
6271 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2464 2494 2527 2557 2641
Size: 30 165 30 1061 30 33 30 84
Cuts at: 2641 2671 1178
Size: 30 6008
Fragments arranged by size:
6008 1061 165 84 33 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2469 2628 3207 4209 1186
Size: 1283 159 579 1002 4478
Fragments arranged by size:
4478 1283 1002 579 159
BseYI C'CCAG_C
Cuts at: 4022 6704 7200 4022
Size: 2682 496 4323
Fragments arranged by size:
4323 2682 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4017 4032 4077 4107 4122 1163
Size: 573 2281 15 45 30 15 4542
Fragments arranged by size:
4542 2281 573 45 30 15 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4951 5712 6740 1540
Size: 275 186 2950 761 1028 2301
Fragments arranged by size:
2950 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2797 5562 5647 6718 2797
Size: 2765 85 1071 3580
Fragments arranged by size:
3580 2765 1071 85
BsmI GAATG_Cn'
Cuts at: 2940 3102 3963 4083 5012 5105 2940
Size: 162 861 120 929 93 5336
Fragments arranged by size:
5336 929 861 162 120 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2510 2676 2797 2985 3643 4370 4450
Size: 399 720 166 121 188 658 727 80
Cuts at: 4450 4820 5562 5647 6718 1391
Size: 370 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 727 720 658 399 370
188 166 121 85 80
BspEI T'CCGG_A
Cuts at: 7164 7164
Size: 7501
BspHI T'CATG_A
Cuts at: 2425 5390 7120 2425
Size: 2965 1730 2806
Fragments arranged by size:
2965 2806 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2779 4856 844
Size: 1935 2077 3489
Fragments arranged by size:
3489 2077 1935
BsrBI CCG'CTC
Cuts at: 1394 1823 5388 1394
Size: 429 3565 3507
Fragments arranged by size:
3565 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2651 2955 5996 6170 65
Size: 1183 1403 304 3041 174 1396
Fragments arranged by size:
3041 1403 1396 1183 304 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6145 6332 1700
Size: 110 4335 187 2869
Fragments arranged by size:
4335 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3724 96
Size: 3628 3873
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7501
BssSI C'ACGA_G
Cuts at: 5561 6573 5561
Size: 1012 6489
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2935 4591 794
Size: 2141 1656 3704
Fragments arranged by size:
3704 2141 1656
BstBI TT'CG_AA
Cuts at: 3556 6305 3556
Size: 2749 4752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7501
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 4526 2011
Size: 2515 4986
BstYI r'GATC_y
Cuts at: 1972 4831 5198 5583 5600 7052 7138 7150 7496
Size: 2859 367 385 17 1452 86 12 346
Cuts at: 7496 1972
Size: 1977
Fragments arranged by size:
2859 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 4704 513
Size: 552 939 48 2652 3310
Fragments arranged by size:
3310 2652 939 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2603 487
Size: 683 56 1377 5385
Fragments arranged by size:
5385 1377 683 56
BtsI GCAGTG_nn'
Cuts at: 2714 2887 3003 5017 5811 5831 2714
Size: 173 116 2014 794 20 4384
Fragments arranged by size:
4384 2014 794 173 116 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7501
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4324 4359 529
Size: 35 3760 35 3671
Fragments arranged by size:
3760 3671 35 35
DraI TTT'AAA
Cuts at: 3355 3781 4668 4805 3355
Size: 426 887 137 6051
Fragments arranged by size:
6051 887 426 137
DraIII CAC_nnn'GTG
Cuts at: 2399 2399
Size: 7501
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6508 809
Size: 507 5192 1802
Fragments arranged by size:
5192 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4948 5837 7466
Size: 54 1425 50 275 18 3118 889 1629
Cuts at: 7466 8
Size: 43
Fragments arranged by size:
3118 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4948 1537
Size: 275 3136 4090
Fragments arranged by size:
4090 3136 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4107 5431 1353
Size: 2754 1324 3423
Fragments arranged by size:
3423 2754 1324
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2739 6088 6462 6608 387
Size: 1064 138 1150 3349 374 146 1280
Fragments arranged by size:
3349 1280 1150 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2625 2811 3180 3559 3671 4206 4254
Size: 393 993 186 369 379 112 535 48
Cuts at: 4254 5564 6161 6948 7216 1239
Size: 1310 597 787 268 1524
Fragments arranged by size:
1524 1310 993 787 597 535 393 379
369 268 186 112 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2334 3903 3933 4101 4221 4296 2334
Size: 1569 30 168 120 75 5539
Fragments arranged by size:
5539 1569 168 120 75 30
EcoO109I rG'GnC_Cy
Cuts at: 3191 4239 4366 4367 3191
Size: 1048 127 1 6325
Fragments arranged by size:
6325 1048 127 1
EcoRI G'AATT_C
Cuts at: 4810 5188 4810
Size: 378 7123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4805 4805
Size: 7501
FspI TGC'GCA
Cuts at: 2180 6007 2180
Size: 3827 3674
FspAI rTGC'GCAy
Cuts at: 2180 2180
Size: 7501
HaeII r_GCGC'y
Cuts at: 1678 6648 1678
Size: 4970 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2581 2613 2857 2889 3153
Size: 32 135 32 1009 32 244 32 264
Cuts at: 3153 3185 3589 3608 3621 3640 6142 6174 6216
Size: 32 404 19 13 19 2502 32 42
Cuts at: 6216 6248 1373
Size: 32 2626
Fragments arranged by size:
2626 2502 1009 404 264 244 135 42
32 32 32 32 32 32 32 19
19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2854 3622 4845 5091 669
Size: 988 281 916 768 1223 246 3079
Fragments arranged by size:
3079 1223 988 916 768 281 246
HindIII A'AGCT_T
Cuts at: 748 3078 3843 748
Size: 2330 765 4406
Fragments arranged by size:
4406 2330 765
HpaI GTT'AAC
Cuts at: 2854 5091 2854
Size: 2237 5264
KpnI G_GTAC'C
Cuts at: 4826 4826
Size: 7501
MfeI C'AATT_G
Cuts at: 2037 5100 2037
Size: 3063 4438
MluI A'CGCG_T
Cuts at: 7377 7377
Size: 7501
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2400 4255 6615 6799 1305
Size: 1095 1855 2360 184 2007
Fragments arranged by size:
2360 2007 1855 1095 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5679
Fragments arranged by size:
5679 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4461 4524 5459 5818 5977 518
Size: 648 843 2452 63 935 359 159 2042
Fragments arranged by size:
2452 2042 935 843 648 359 159 63
MspA1I CmG'CkG
Cuts at: 1606 4739 4917 5594 6742 6987 1606
Size: 3133 178 677 1148 245 2120
Fragments arranged by size:
3133 2120 1148 677 245 178
NcoI C'CATG_G
Cuts at: 513 1065 2004 2052 4704 513
Size: 552 939 48 2652 3310
Fragments arranged by size:
3310 2652 939 552 48
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7501
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7501
NotI GC'GGCC_GC
Cuts at: 4948 4948
Size: 7501
NspI r_CATG'y
Cuts at: 1132 1229 2737 3341 3731 4121 4594 4859 6404
Size: 97 1508 604 390 390 473 265 1545
Cuts at: 6404 1132
Size: 2229
Fragments arranged by size:
2229 1545 1508 604 473 390 390 265
97
PasI CC'CwG_GG
Cuts at: 1348 4451 1348
Size: 3103 4398
PciI A'CATG_T
Cuts at: 3337 3727 6400 3337
Size: 390 2673 4438
Fragments arranged by size:
4438 2673 390
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4478 1108
Size: 3370 4131
PfoI T'CCnGG_A
Cuts at: 3952 4222 3952
Size: 270 7231
PmeI GTTT'AAAC
Cuts at: 4805 4805
Size: 7501
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2496 5570 5602 7083 7115 2464
Size: 32 3074 32 1481 32 2850
Fragments arranged by size:
3074 2850 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3191 3191
Size: 7501
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7501
PsiI TTA'TAA
Cuts at: 5071 5071
Size: 7501
PspOMI G'GGCC_C
Cuts at: 4366 4366
Size: 7501
PstI C_TGCA'G
Cuts at: 830 3570 3949 4853 830
Size: 2740 379 904 3478
Fragments arranged by size:
3478 2740 904 379
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7501
PvuII CAG'CTG
Cuts at: 4739 4739
Size: 7501
SalI G'TCGA_C
Cuts at: 1936 4843 1936
Size: 2907 4594
SapI GCTCTTCn'nnn_
Cuts at: 4107 4107
Size: 7501
SbfI CC_TGCA'GG
Cuts at: 4853 4853
Size: 7501
ScaI AGT'ACT
Cuts at: 5749 7461 5749
Size: 1712 5789
SfcI C'TryA_G
Cuts at: 826 1045 3566 3945 4131 4767 4849 5984 6665
Size: 219 2521 379 186 636 82 1135 681
Cuts at: 6665 6856 826
Size: 191 1471
Fragments arranged by size:
2521 1471 1135 681 636 379 219 191
186 82
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7501
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7501
SmaI CCC'GGG
Cuts at: 1872 4413 4828 1872
Size: 2541 415 4545
Fragments arranged by size:
4545 2541 415
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3063 5605 6506 6768 820
Size: 197 926 1120 2542 901 262 1553
Fragments arranged by size:
2542 1553 1120 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5292 493
Size: 4799 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7501
SphI G_CATG'C
Cuts at: 1132 2737 4121 4859 1132
Size: 1605 1384 738 3774
Fragments arranged by size:
3774 1605 1384 738
SspI AAT'ATT
Cuts at: 5 52 3655 5425 5
Size: 47 3603 1770 2081
Fragments arranged by size:
3603 2081 1770 47
StuI AGG'CCT
Cuts at: 1517 4017 4258 4765 1517
Size: 2500 241 507 4253
Fragments arranged by size:
4253 2500 507 241
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 3904 4704 4940 513
Size: 552 939 48 1852 800 236 3074
Fragments arranged by size:
3074 1852 939 800 552 236 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2114 2410 2719 2737 4451 4582 5528
Size: 1255 534 296 309 18 1714 131 946
Cuts at: 5528 5545 5698 5883 6328 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1714 1498 1255 946 534 445 309 296
185 153 131 18 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3483 3724 5747
Size: 274 80 33 51 1443 1506 241 2023
Cuts at: 5747 7459 96
Size: 1712 138
Fragments arranged by size:
2023 1712 1506 1443 274 241 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2527 2559 3693 3725 7461 7493 2527
Size: 32 1134 32 3736 32 2535
Fragments arranged by size:
3736 2535 1134 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7501
XbaI T'CTAG_A
Cuts at: 4837 4837
Size: 7501
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2072 2072
Size: 7501
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7501
XmaI C'CCGG_G
Cuts at: 1870 4411 4826 1870
Size: 2541 415 4545
Fragments arranged by size:
4545 2541 415
XmnI GAAnn'nnTTC
Cuts at: 5630 5630
Size: 7501
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5309 276
Size: 53 83 186 936 3775 2468
Fragments arranged by size:
3775 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI