Restriction map for
pFN21AA0761
(Circular) (Six-base) MAPSORT of: pFN21AA0761.seq Check: 728 from: 1 to: 6358
pFN21AA0761 6358 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3173 3173
Size: 6358
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4168 278
Size: 53 83 186 936 2632 2468
Fragments arranged by size:
2632 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3701 1937
Size: 1764 4594
Acc65I G'GTAC_C
Cuts at: 3379 3679 3379
Size: 300 6058
AclI AA'CG_TT
Cuts at: 1563 4485 4858 1563
Size: 2922 373 3063
Fragments arranged by size:
3063 2922 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2094 2751 3342 3582 4421 5805 6073 1632
Size: 462 657 591 240 839 1384 268 1917
Fragments arranged by size:
1917 1384 839 657 591 462 268 240
AfeI AGC'GCT
Cuts at: 3000 3000
Size: 6358
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6161
AflIII A'CryG_T
Cuts at: 1203 2647 5257 6234 1203
Size: 1444 2610 977 1327
Fragments arranged by size:
2610 1444 1327 977
AgeI A'CCGG_T
Cuts at: 5189 5189
Size: 6358
AhdI GACnn_n'nnGTC
Cuts at: 5087 5087
Size: 6358
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6358
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2391 1184
Size: 1207 5151
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3057 3089 3057
Size: 32 6326
AlwNI CAG_nnn'CTG
Cuts at: 3651 4101 5673 3651
Size: 450 1572 4336
Fragments arranged by size:
4336 1572 450
ApaI G_GGCC'C
Cuts at: 3221 3221
Size: 6358
ApaLI G'TGCA_C
Cuts at: 4415 5571 4415
Size: 1156 5202
ApoI r'AATT_y
Cuts at: 1493 2966 2991 3667 3893 4045 6154 1493
Size: 1473 25 676 226 152 2109 1697
Fragments arranged by size:
2109 1697 1473 676 226 152 25
AseI AT'TA_AT
Cuts at: 160 1032 2484 4912 160
Size: 872 1452 2428 1606
Fragments arranged by size:
2428 1606 1452 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
AvaI C'yCGr_G
Cuts at: 1870 1943 3683 1870
Size: 73 1740 4545
Fragments arranged by size:
4545 1740 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3363 3396 3363
Size: 33 6325
BamHI G'GATC_C
Cuts at: 2545 3634 3688 4055 2545
Size: 1089 54 367 4848
Fragments arranged by size:
4848 1089 367 54
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2450 3379 3679 5134 618
Size: 325 217 627 663 929 300 1455 1842
Fragments arranged by size:
1842 1455 929 663 627 325 300 217
BanII G_rGCy'C
Cuts at: 1391 2859 3221 3320 3677 1391
Size: 1468 362 99 357 4072
Fragments arranged by size:
4072 1468 362 357 99
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2271 2442 3148 928
Size: 971 372 171 706 4138
Fragments arranged by size:
4138 971 706 372 171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4549 4583 1903
Size: 34 50 34 2528 34 3678
Fragments arranged by size:
3678 2528 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3400 4250 5460 3400
Size: 850 1210 4298
Fragments arranged by size:
4298 1210 850
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6358
BfrBI ATG'CAT
Cuts at: 2368 2368
Size: 6358
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3216 3654 4969
Size: 107 122 71 631 605 1544 438 1315
Cuts at: 4969 136
Size: 1525
Fragments arranged by size:
1544 1525 1315 631 605 438 122 107
71
BglII A'GATC_T
Cuts at: 6353 6353
Size: 6358
BlpI GC'TnA_GC
Cuts at: 3775 3775
Size: 6358
Bme1580I G_kGCm'C
Cuts at: 1790 3221 4419 5575 1790
Size: 1431 1198 1156 2573
Fragments arranged by size:
2573 1431 1198 1156
BmgBI CAC'GTC
Cuts at: 1318 2648 1318
Size: 1330 5028
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2970 3378 4089
Size: 416 53 83 399 24 1553 408 711
Cuts at: 4089 5047 442
Size: 958 1753
Fragments arranged by size:
1753 1553 958 711 416 408 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2711 1056
Size: 1655 4703
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2145 3129 2145
Size: 984 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5018 1239
Size: 3779 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3313 3539 4483 5348 5646 3313
Size: 226 944 865 298 4025
Fragments arranged by size:
4025 944 865 298 226
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5021 882
Size: 617 3522 2219
Fragments arranged by size:
3522 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3626 4149 493
Size: 711 2422 523 2702
Fragments arranged by size:
2702 2422 711 523
BsaBI GATnn'nnATC
Cuts at: 6352 6352
Size: 6358
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4165 4547 5182 275
Size: 53 83 186 936 2632 382 635 1451
Fragments arranged by size:
2632 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3637 4791 5189 5463 5610 6021 3637
Size: 1154 398 274 147 411 3974
Fragments arranged by size:
3974 1154 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6133
Fragments arranged by size:
6133 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6358
BseYI C'CCAG_C
Cuts at: 3549 5561 6057 3549
Size: 2012 496 3850
Fragments arranged by size:
3850 2012 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5785
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3808 4569 5597 1540
Size: 275 186 1807 761 1028 2301
Fragments arranged by size:
2301 1807 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2859 3330 4419 4504 5575 2859
Size: 471 1089 85 1071 3642
Fragments arranged by size:
3642 1089 1071 471 85
BsmI GAATG_Cn'
Cuts at: 2036 3869 3962 2036
Size: 1833 93 4432
Fragments arranged by size:
4432 1833 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2638 2638
Size: 6358
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2859 3221 3320 3330 3677 4419 4504
Size: 399 1069 362 99 10 347 742 85
Cuts at: 4504 5575 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1069 742 399 362 347 99
85 10
BspEI T'CCGG_A
Cuts at: 6021 6021
Size: 6358
BspHI T'CATG_A
Cuts at: 2059 2497 2982 4247 5977 2059
Size: 438 485 1265 1730 2440
Fragments arranged by size:
2440 1730 1265 485 438
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3173 3713 844
Size: 2329 540 3489
Fragments arranged by size:
3489 2329 540
BsrBI CCG'CTC
Cuts at: 1394 1823 4245 1394
Size: 429 2422 3507
Fragments arranged by size:
3507 2422 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4853 5027 65
Size: 1183 3605 174 1396
Fragments arranged by size:
3605 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5002 5189 1700
Size: 110 3192 187 2869
Fragments arranged by size:
3192 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6358
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6358
BssSI C'ACGA_G
Cuts at: 3360 4418 5430 3360
Size: 1058 1012 4288
Fragments arranged by size:
4288 1058 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3188 794
Size: 2394 3964
BstBI TT'CG_AA
Cuts at: 2526 5162 2526
Size: 2636 3722
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6358
BstXI CCAn_nnnn'nTGG
Cuts at: 2776 2776
Size: 6358
BstYI r'GATC_y
Cuts at: 1972 2545 3634 3688 4055 4440 4457 5909 5995
Size: 573 1089 54 367 385 17 1452 86
Cuts at: 5995 6007 6353 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1089 573 385 367 346 86
54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3131 3372 513
Size: 552 2066 241 3499
Fragments arranged by size:
3499 2066 552 241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5619
Fragments arranged by size:
5619 683 56
BtsI GCAGTG_nn'
Cuts at: 3874 4668 4688 3874
Size: 794 20 5544
Fragments arranged by size:
5544 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6358
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3594 3629 529
Size: 35 3030 35 3258
Fragments arranged by size:
3258 3030 35 35
DraI TTT'AAA
Cuts at: 3662 3662
Size: 6358
DraIII CAC_nnn'GTG
Cuts at: 2471 2471
Size: 6358
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5365 809
Size: 507 4049 1802
Fragments arranged by size:
4049 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3128 3483 3499
Size: 54 1425 50 275 18 1298 355 16
Cuts at: 3499 3805 4694 6323 8
Size: 306 889 1629 43
Fragments arranged by size:
1629 1425 1298 889 355 306 275 54
50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 3805 1537
Size: 275 1993 4090
Fragments arranged by size:
4090 1993 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3256 4288 1353
Size: 1903 1032 3423
Fragments arranged by size:
3423 1903 1032
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4945 5319 5465 387
Size: 1064 138 3356 374 146 1280
Fragments arranged by size:
3356 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2857 2857
Size: 6358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2751 3342 3582 4421 5018 5805
Size: 393 462 657 591 240 839 597 787
Cuts at: 5805 6073 1239
Size: 268 1524
Fragments arranged by size:
1524 839 787 657 597 591 462 393
268 240
EcoO109I rG'GnC_Cy
Cuts at: 3218 3233 3218
Size: 15 6343
EcoRI G'AATT_C
Cuts at: 3667 4045 3667
Size: 378 5980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2418 2418
Size: 6358
FspI TGC'GCA
Cuts at: 4864 4864
Size: 6358
HaeII r_GCGC'y
Cuts at: 1678 3002 5505 1678
Size: 1324 2503 2531
Fragments arranged by size:
2531 2503 1324
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2180 2212 4999 5031 5073
Size: 32 135 32 608 32 2787 32 42
Cuts at: 5073 5105 1373
Size: 32 2626
Fragments arranged by size:
2787 2626 608 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2869 3289 3702 3948 669
Size: 988 281 931 420 413 246 3079
Fragments arranged by size:
3079 988 931 420 413 281 246
HindIII A'AGCT_T
Cuts at: 748 2333 748
Size: 1585 4773
HpaI GTT'AAC
Cuts at: 3948 3948
Size: 6358
KpnI G_GTAC'C
Cuts at: 3383 3683 3383
Size: 300 6058
MfeI C'AATT_G
Cuts at: 3957 3957
Size: 6358
MluI A'CGCG_T
Cuts at: 6234 6234
Size: 6358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5472 5656 1305
Size: 4167 184 2007
Fragments arranged by size:
4167 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3501 10
Size: 54 1425 343 1669 2867
Fragments arranged by size:
2867 1669 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2064 2087 2373 2515 4316 4675 4834
Size: 648 898 23 286 142 1801 359 159
Cuts at: 4834 518
Size: 2042
Fragments arranged by size:
2042 1801 898 648 359 286 159 142
23
MspA1I CmG'CkG
Cuts at: 1606 3539 3774 4451 5599 5844 1606
Size: 1933 235 677 1148 245 2120
Fragments arranged by size:
2120 1933 1148 677 245 235
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5806
NdeI CA'TA_TG
Cuts at: 387 2620 387
Size: 2233 4125
NheI G'CTAG_C
Cuts at: 1052 2707 1052
Size: 1655 4703
NotI GC'GGCC_GC
Cuts at: 3805 3805
Size: 6358
NsiI A_TGCA'T
Cuts at: 2370 2370
Size: 6358
NspI r_CATG'y
Cuts at: 1132 1229 2092 3716 5261 1132
Size: 97 863 1624 1545 2229
Fragments arranged by size:
2229 1624 1545 863 97
PacI TTA_AT'TAA
Cuts at: 2484 2484
Size: 6358
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6358
PciI A'CATG_T
Cuts at: 5257 5257
Size: 6358
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6358
PmeI GTTT'AAAC
Cuts at: 3662 3662
Size: 6358
PmlI CAC'GTG
Cuts at: 3626 3626
Size: 6358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4427 4459 5940 5972 4427
Size: 32 1481 32 4813
Fragments arranged by size:
4813 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6358
PsiI TTA'TAA
Cuts at: 2232 3928 2232
Size: 1696 4662
PspOMI G'GGCC_C
Cuts at: 3217 3217
Size: 6358
PstI C_TGCA'G
Cuts at: 830 3710 830
Size: 2880 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6358
SacI G_AGCT'C
Cuts at: 2859 2859
Size: 6358
SalI G'TCGA_C
Cuts at: 1936 3700 1936
Size: 1764 4594
SbfI CC_TGCA'GG
Cuts at: 3710 3710
Size: 6358
ScaI AGT'ACT
Cuts at: 3466 4606 6318 3466
Size: 1140 1712 3506
Fragments arranged by size:
3506 1712 1140
SexAI A'CCwGG_T
Cuts at: 2446 2446
Size: 6358
SfcI C'TryA_G
Cuts at: 826 1045 3706 4841 5522 5713 826
Size: 219 2661 1135 681 191 1471
Fragments arranged by size:
2661 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3216 3216
Size: 6358
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6358
SmaI CCC'GGG
Cuts at: 1872 3685 1872
Size: 1813 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3292 3554 4462 5363 5625 820
Size: 197 926 1349 262 908 901 262 1553
Fragments arranged by size:
1553 1349 926 908 901 262 262 197
SnaBI TAC'GTA
Cuts at: 493 4149 493
Size: 3656 2702
SpeI A'CTAG_T
Cuts at: 152 2863 152
Size: 2711 3647
SphI G_CATG'C
Cuts at: 1132 3716 1132
Size: 2584 3774
SspI AAT'ATT
Cuts at: 5 52 4282 5
Size: 47 4230 2081
Fragments arranged by size:
4230 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6358
StyI C'CwwG_G
Cuts at: 513 1065 2454 3417 3583 3797 513
Size: 552 1389 963 166 214 3074
Fragments arranged by size:
3074 1389 963 552 214 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4385 4402 4555 4740 5185 325
Size: 1255 2805 17 153 185 445 1498
Fragments arranged by size:
2805 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3464 4604 6316
Size: 274 80 33 51 1443 1487 1140 1712
Cuts at: 6316 96
Size: 138
Fragments arranged by size:
1712 1487 1443 1140 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2434 2466 6318 6350 2434
Size: 32 3852 32 2442
Fragments arranged by size:
3852 2442 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6358
XbaI T'CTAG_A
Cuts at: 3694 3694
Size: 6358
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6358
XmaI C'CCGG_G
Cuts at: 1870 3683 1870
Size: 1813 4545
XmnI GAAnn'nnTTC
Cuts at: 4487 4487
Size: 6358
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4166 276
Size: 53 83 186 936 2632 2468
Fragments arranged by size:
2632 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PfoI PpuMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SfoI SrfI SwaI XcmI