Restriction map for
pFN21AA0756
(Circular) (Six-base) MAPSORT of: pFN21AA0756.seq Check: 2090 from: 1 to: 8212
pFN21AA0756 8212 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2380 5017 2380
Size: 2637 5575
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6022 278
Size: 53 83 186 936 4486 2468
Fragments arranged by size:
4486 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4371 4539 5555 1937
Size: 2434 168 1016 4594
Fragments arranged by size:
4594 2434 1016 168
Acc65I G'GTAC_C
Cuts at: 5533 5533
Size: 8212
AclI AA'CG_TT
Cuts at: 1563 6339 6712 1563
Size: 4776 373 3063
Fragments arranged by size:
4776 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3731 4503 4508 4628 5265 6275 7659 7927
Size: 2099 772 5 120 637 1010 1384 268
Cuts at: 7927 1632
Size: 1917
Fragments arranged by size:
2099 1917 1384 1010 772 637 268 120
5
AfeI AGC'GCT
Cuts at: 4148 4148
Size: 8212
AflII C'TTAA_G
Cuts at: 820 1017 2716 820
Size: 197 1699 6316
Fragments arranged by size:
6316 1699 197
AflIII A'CryG_T
Cuts at: 1203 2548 2733 3807 3809 7111 8088 1203
Size: 1345 185 1074 2 3302 977 1327
Fragments arranged by size:
3302 1345 1327 1074 977 185 2
AgeI A'CCGG_T
Cuts at: 7043 7043
Size: 8212
AhdI GACnn_n'nnGTC
Cuts at: 4990 6941 4990
Size: 1951 6261
AleI CACnn'nnGTG
Cuts at: 1166 3650 1166
Size: 2484 5728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3668 1184
Size: 2484 5728
AlwNI CAG_nnn'CTG
Cuts at: 5955 7527 5955
Size: 1572 6640
ApaI G_GGCC'C
Cuts at: 4424 4424
Size: 8212
ApaLI G'TGCA_C
Cuts at: 6269 7425 6269
Size: 1156 7056
ApoI r'AATT_y
Cuts at: 1493 2082 2354 5521 5747 5899 8008 1493
Size: 589 272 3167 226 152 2109 1697
Fragments arranged by size:
3167 2109 1697 589 272 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6766 160
Size: 872 5734 1606
Fragments arranged by size:
5734 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8212
AvaI C'yCGr_G
Cuts at: 1870 1943 2250 2745 3620 3885 3941 4172 4308
Size: 73 307 495 875 265 56 231 136
Cuts at: 4308 5537 1870
Size: 1229 4545
Fragments arranged by size:
4545 1229 875 495 307 265 231 136
73 56
AvrII C'CTAG_G
Cuts at: 4796 4796
Size: 8212
BamHI G'GATC_C
Cuts at: 2379 3936 5542 5909 2379
Size: 1557 1606 367 4682
Fragments arranged by size:
4682 1606 1557 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2789 3175 3644 5330 5533
Size: 325 217 627 1002 386 469 1686 203
Cuts at: 5533 6988 618
Size: 1455 1842
Fragments arranged by size:
1842 1686 1455 1002 627 469 386 325
217 203
BanII G_rGCy'C
Cuts at: 1391 2532 4343 4424 5036 5531 1391
Size: 1141 1811 81 612 495 4072
Fragments arranged by size:
4072 1811 1141 612 495 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2970 3762 3999 5253 5409 928
Size: 971 1071 792 237 1254 156 3731
Fragments arranged by size:
3731 1254 1071 971 792 237 156
BbvCI CC'TCA_GC
Cuts at: 5012 5012
Size: 8212
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6403 6437 1903
Size: 34 4466 34 3678
Fragments arranged by size:
4466 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2790 4877 6104 7314 2790
Size: 2087 1227 1210 3688
Fragments arranged by size:
3688 2087 1227 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8212
BfrBI ATG'CAT
Cuts at: 2841 2841
Size: 8212
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3880 4256 4668
Size: 107 122 71 631 605 2208 376 412
Cuts at: 4668 6823 136
Size: 2155 1525
Fragments arranged by size:
2208 2155 1525 631 605 412 376 122
107 71
BglII A'GATC_T
Cuts at: 8207 8207
Size: 8212
BlpI GC'TnA_GC
Cuts at: 5629 5629
Size: 8212
Bme1580I G_kGCm'C
Cuts at: 1790 2746 2792 3647 4380 4424 4795 6273 7429
Size: 956 46 855 733 44 371 1478 1156
Cuts at: 7429 1790
Size: 2573
Fragments arranged by size:
2573 1478 1156 956 855 733 371 46
44
BmgBI CAC'GTC
Cuts at: 1318 4192 5481 1318
Size: 2874 1289 4049
Fragments arranged by size:
4049 2874 1289
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4361 4782 5204
Size: 416 53 83 399 24 2944 421 422
Cuts at: 5204 5943 6901 442
Size: 739 958 1753
Fragments arranged by size:
2944 1753 958 739 422 421 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8212
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2983 4323 5284 2983
Size: 1340 961 5911
Fragments arranged by size:
5911 1340 961
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 3605 3680 3914 4609 4730 4847 6872
Size: 1211 1155 75 234 695 121 117 2025
Cuts at: 6872 1239
Size: 2579
Fragments arranged by size:
2579 2025 1211 1155 695 234 121 117
75
Bpu10I CC'TnA_GC
Cuts at: 4425 4857 5012 4425
Size: 432 155 7625
Fragments arranged by size:
7625 432 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2663 6337 7202 7500 2663
Size: 3674 865 298 3375
Fragments arranged by size:
3674 3375 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2565 3411 4748 6875 882
Size: 617 1066 846 1337 2127 2219
Fragments arranged by size:
2219 2127 1337 1066 846 617
BsaAI yAC'GTr
Cuts at: 493 1204 3810 4233 4351 4375 6003 493
Size: 711 2606 423 118 24 1628 2702
Fragments arranged by size:
2702 2606 1628 711 423 118 24
BsaBI GATnn'nnATC
Cuts at: 8206 8206
Size: 8212
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4238 6019 6401 7036
Size: 53 83 186 936 2705 1781 382 635
Cuts at: 7036 275
Size: 1451
Fragments arranged by size:
2705 1781 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3375 3612 4451 6645 7043 7317 7464 7875 3375
Size: 237 839 2194 398 274 147 411 3712
Fragments arranged by size:
3712 2194 839 411 398 274 237 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3668 3698 4217 4247 1178
Size: 30 165 30 2265 30 519 30 5143
Fragments arranged by size:
5143 2265 519 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2048 2278 2281 2394 2763 3931 5069
Size: 853 9 230 3 113 369 1168 1138
Cuts at: 5069 5203 1186
Size: 134 4195
Fragments arranged by size:
4195 1168 1138 853 369 230 134 113
9 3
BseYI C'CCAG_C
Cuts at: 2198 4130 7415 7911 2198
Size: 1932 3285 496 2499
Fragments arranged by size:
3285 2499 1932 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3674 1163
Size: 573 1938 5701
Fragments arranged by size:
5701 1938 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2312 5662 6423 7451 1540
Size: 275 186 311 3350 761 1028 2301
Fragments arranged by size:
3350 2301 1028 761 311 275 186
BsiHKAI G_wGCw'C
Cuts at: 4168 6273 6358 7429 4168
Size: 2105 85 1071 4951
Fragments arranged by size:
4951 2105 1071 85
BsmI GAATG_Cn'
Cuts at: 2843 4252 5723 5816 2843
Size: 1409 1471 93 5239
Fragments arranged by size:
5239 1471 1409 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3706 3706
Size: 8212
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2532 2746 2792 3647 4168 4343 4380
Size: 399 742 214 46 855 521 175 37
Cuts at: 4380 4424 4795 5036 5531 6273 6358 7429 1391
Size: 44 371 241 495 742 85 1071 2174
Fragments arranged by size:
2174 1071 855 742 742 521 495 399
371 241 214 175 85 46 44 37
BspEI T'CCGG_A
Cuts at: 4451 7875 4451
Size: 3424 4788
BspHI T'CATG_A
Cuts at: 4500 4875 6101 7831 4500
Size: 375 1226 1730 4881
Fragments arranged by size:
4881 1730 1226 375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2380 3294 4281 5017 5567 844
Size: 1536 914 987 736 550 3489
Fragments arranged by size:
3489 1536 987 914 736 550
BsrBI CCG'CTC
Cuts at: 1394 1823 3727 3790 6099 1394
Size: 429 1904 63 2309 3507
Fragments arranged by size:
3507 2309 1904 429 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 4098 6707 6881 65
Size: 1183 2850 2609 174 1396
Fragments arranged by size:
2850 2609 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3879 6856 7043 1700
Size: 110 2069 2977 187 2869
Fragments arranged by size:
2977 2869 2069 187 110
BsrGI T'GTAC_A
Cuts at: 96 2736 96
Size: 2640 5572
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8212
BssSI C'ACGA_G
Cuts at: 6272 7284 6272
Size: 1012 7200
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2605 794
Size: 1811 6401
BstBI TT'CG_AA
Cuts at: 7016 7016
Size: 8212
BstEII G'GTnAC_C
Cuts at: 1184 4655 1184
Size: 3471 4741
BstXI CCAn_nnnn'nTGG
Cuts at: 3001 3556 3001
Size: 555 7657
BstYI r'GATC_y
Cuts at: 1972 2092 2379 3606 3936 4386 5542 5909 6294
Size: 120 287 1227 330 450 1156 367 385
Cuts at: 6294 6311 7763 7849 7861 8207 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1227 1156 450 385 367 346
330 287 120 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2523 3648 4666 513
Size: 552 939 519 1125 1018 4059
Fragments arranged by size:
4059 1125 1018 939 552 519
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2221 2827 2854 3439 5089 5128
Size: 683 56 995 606 27 585 1650 39
Cuts at: 5128 487
Size: 3571
Fragments arranged by size:
3571 1650 995 683 606 585 56 39
27
BtsI GCAGTG_nn'
Cuts at: 2305 3419 4756 5728 6522 6542 2305
Size: 1114 1337 972 794 20 3975
Fragments arranged by size:
3975 1337 1114 972 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2869 2904 529
Size: 35 2305 35 5837
Fragments arranged by size:
5837 2305 35 35
DraI TTT'AAA
Cuts at: 5516 5516
Size: 8212
DraIII CAC_nnn'GTG
Cuts at: 2143 4335 2143
Size: 2192 6020
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2549 3108 3977 7219 809
Size: 507 1233 559 869 3242 1802
Fragments arranged by size:
3242 1802 1233 869 559 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2309 3201
Size: 54 1425 50 275 18 177 302 892
Cuts at: 3201 3291 3633 3843 3872 3909 5419 5508 5659
Size: 90 342 210 29 37 1510 89 151
Cuts at: 5659 6548 8177 8
Size: 889 1629 43
Fragments arranged by size:
1629 1510 1425 892 889 342 302 275
210 177 151 90 89 54 50 43
37 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 2309 5659 1537
Size: 275 497 3350 4090
Fragments arranged by size:
4090 3350 497 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4625 4691 5253 6142 1353
Size: 3272 66 562 889 3423
Fragments arranged by size:
3423 3272 889 562 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2085 2372 3569 4312 6799 7173
Size: 1064 138 496 287 1197 743 2487 374
Cuts at: 7173 7319 387
Size: 146 1280
Fragments arranged by size:
2487 1280 1197 1064 743 496 374 287
146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 3605 3680 3731 3914 4503 4508
Size: 393 818 1155 75 51 183 589 5
Cuts at: 4508 4609 4628 4730 4847 5265 6275 6872 7659
Size: 101 19 102 117 418 1010 597 787
Cuts at: 7659 7927 1239
Size: 268 1524
Fragments arranged by size:
1524 1155 1010 818 787 597 589 418
393 268 183 117 102 101 75 51
19 5
EcoNI CCTnn'n_nnAGG
Cuts at: 3845 3845
Size: 8212
EcoO109I rG'GnC_Cy
Cuts at: 2245 2277 2683 2854 2893 2935 3889 4420 4421
Size: 32 406 171 39 42 954 531 1
Cuts at: 4421 2245
Size: 6036
Fragments arranged by size:
6036 954 531 406 171 42 39 32
1
EcoRI G'AATT_C
Cuts at: 5521 5899 5521
Size: 378 7834
FspI TGC'GCA
Cuts at: 6718 6718
Size: 8212
HaeII r_GCGC'y
Cuts at: 1678 2454 4150 5019 7359 1678
Size: 776 1696 869 2340 2531
Fragments arranged by size:
2531 2340 1696 869 776
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2885 2917 3198 3230 4418
Size: 32 135 32 1313 32 281 32 1188
Cuts at: 4418 4450 4875 4907 4976 5008 5465 5497 6853
Size: 32 425 32 69 32 457 32 1356
Cuts at: 6853 6885 6927 6959 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1356 1313 1188 457 425 281 135
69 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4048 4081 5556 5802 669
Size: 988 281 2110 33 1475 246 3079
Fragments arranged by size:
3079 2110 1475 988 281 246 33
HindIII A'AGCT_T
Cuts at: 748 2770 5079 748
Size: 2022 2309 3881
Fragments arranged by size:
3881 2309 2022
HpaI GTT'AAC
Cuts at: 4048 5802 4048
Size: 1754 6458
KpnI G_GTAC'C
Cuts at: 5537 5537
Size: 8212
MfeI C'AATT_G
Cuts at: 5811 5811
Size: 8212
MluI A'CGCG_T
Cuts at: 8088 8088
Size: 8212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2610 3018 3294 4027 4569 7326 7510 1305
Size: 1305 408 276 733 542 2757 184 2007
Fragments arranged by size:
2757 2007 1305 733 542 408 276 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 3293 3635 3845 10
Size: 54 1425 343 177 1284 342 210 4377
Fragments arranged by size:
4377 1425 1284 343 342 210 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3359 3424 3477 3650 4866 6170 6529
Size: 648 2193 65 53 173 1216 1304 359
Cuts at: 6529 6688 518
Size: 159 2042
Fragments arranged by size:
2193 2042 1304 1216 648 359 173 159
65 53
MspA1I CmG'CkG
Cuts at: 1606 3658 5628 6305 7453 7698 1606
Size: 2052 1970 677 1148 245 2120
Fragments arranged by size:
2120 2052 1970 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2523 513
Size: 552 939 519 6202
Fragments arranged by size:
6202 939 552 519
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8212
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8212
NotI GC'GGCC_GC
Cuts at: 5659 5659
Size: 8212
NsiI A_TGCA'T
Cuts at: 2843 2843
Size: 8212
NspI r_CATG'y
Cuts at: 1132 1229 2608 2737 5570 7115 1132
Size: 97 1379 129 2833 1545 2229
Fragments arranged by size:
2833 2229 1545 1379 129 97
PasI CC'CwG_GG
Cuts at: 1348 2028 2785 4467 5113 1348
Size: 680 757 1682 646 4447
Fragments arranged by size:
4447 1682 757 680 646
PciI A'CATG_T
Cuts at: 2733 7111 2733
Size: 4378 3834
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3148 3841 1108
Size: 2040 693 5479
Fragments arranged by size:
5479 2040 693
PfoI T'CCnGG_A
Cuts at: 3222 3222
Size: 8212
PmeI GTTT'AAAC
Cuts at: 5516 5516
Size: 8212
PmlI CAC'GTG
Cuts at: 3810 4233 3810
Size: 423 7789
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5378 5410 6281 6313 7794 7826 5378
Size: 32 871 32 1481 32 5764
Fragments arranged by size:
5764 1481 871 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2245 2277 2683 2854 2893 3889 2245
Size: 32 406 171 39 996 6568
Fragments arranged by size:
6568 996 406 171 39 32
PshAI GACnn'nnGTC
Cuts at: 1613 4367 1613
Size: 2754 5458
PsiI TTA'TAA
Cuts at: 2703 5782 2703
Size: 3079 5133
PspOMI G'GGCC_C
Cuts at: 4420 4420
Size: 8212
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4523 4555 4523
Size: 32 8180
PstI C_TGCA'G
Cuts at: 830 2391 2601 5307 5564 830
Size: 1561 210 2706 257 3478
Fragments arranged by size:
3478 2706 1561 257 210
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8212
PvuII CAG'CTG
Cuts at: 3658 3658
Size: 8212
SalI G'TCGA_C
Cuts at: 1936 5554 1936
Size: 3618 4594
SanDI GG'GwC_CC
Cuts at: 2277 2854 2277
Size: 577 7635
SbfI CC_TGCA'GG
Cuts at: 2391 5307 5564 2391
Size: 2916 257 5039
Fragments arranged by size:
5039 2916 257
ScaI AGT'ACT
Cuts at: 6460 8172 6460
Size: 1712 6500
SexAI A'CCwGG_T
Cuts at: 3627 3627
Size: 8212
SfcI C'TryA_G
Cuts at: 826 1045 2387 2597 2617 4693 5278 5303 5560
Size: 219 1342 210 20 2076 585 25 257
Cuts at: 5560 6695 7376 7567 826
Size: 1135 681 191 1471
Fragments arranged by size:
2076 1471 1342 1135 681 585 257 219
210 191 25 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 3880 3880
Size: 8212
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8212
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8212
SmaI CCC'GGG
Cuts at: 1872 2252 3887 3943 5539 1872
Size: 380 1635 56 1596 4545
Fragments arranged by size:
4545 1635 1596 380 56
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2678 2716 4308 6316 7217 7479
Size: 197 926 735 38 1592 2008 901 262
Cuts at: 7479 820
Size: 1553
Fragments arranged by size:
2008 1592 1553 926 901 735 262 197
38
SnaBI TAC'GTA
Cuts at: 493 6003 493
Size: 5510 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8212
SphI G_CATG'C
Cuts at: 1132 5570 1132
Size: 4438 3774
SspI AAT'ATT
Cuts at: 5 52 6136 5
Size: 47 6084 2081
Fragments arranged by size:
6084 2081 47
StuI AGG'CCT
Cuts at: 1517 3851 4316 1517
Size: 2334 465 5413
Fragments arranged by size:
5413 2334 465
StyI C'CwwG_G
Cuts at: 513 1065 2004 2241 2415 2523 3846 4758 4796
Size: 552 939 237 174 108 1323 912 38
Cuts at: 4796 5236 5253 5651 513
Size: 440 17 398 3074
Fragments arranged by size:
3074 1323 939 912 552 440 398 237
174 108 38 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2552 2627 2660 3245 3917 4609 5126
Size: 1255 972 75 33 585 672 692 517
Cuts at: 5126 6239 6256 6409 6594 7039 325
Size: 1113 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1113 972 692 672 585 517
445 185 153 75 33 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2736 5424 6458
Size: 274 80 33 51 1443 759 2688 1034
Cuts at: 6458 8170 96
Size: 1712 138
Fragments arranged by size:
2688 1712 1443 1034 759 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2321 2353 3668 3700 4217 4249 8172 8204 2321
Size: 32 1315 32 517 32 3923 32 2329
Fragments arranged by size:
3923 2329 1315 517 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2432 3974 4550 1316
Size: 1116 1542 576 4978
Fragments arranged by size:
4978 1542 1116 576
XbaI T'CTAG_A
Cuts at: 5548 5548
Size: 8212
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2025 2871 3553 3655 3778 4004 5026 5149 2025
Size: 846 682 102 123 226 1022 123 5088
Fragments arranged by size:
5088 1022 846 682 226 123 123 102
XhoI C'TCGA_G
Cuts at: 1943 4308 1943
Size: 2365 5847
XmaI C'CCGG_G
Cuts at: 1870 2250 3885 3941 5537 1870
Size: 380 1635 56 1596 4545
Fragments arranged by size:
4545 1635 1596 380 56
XmnI GAAnn'nnTTC
Cuts at: 2669 6341 2669
Size: 3672 4540
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6020 276
Size: 53 83 186 936 4486 2468
Fragments arranged by size:
4486 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BsiWI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PspXI RsrII SacI SacII SapI SfoI
SrfI SwaI