Restriction map for
pFN21AA0751
(Circular) (Six-base) MAPSORT of: pFN21AA0751.seq Check: 6825 from: 1 to: 8269
pFN21AA0751 8269 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4741 5110 4741
Size: 369 7900
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6079 278
Size: 53 83 186 936 4543 2468
Fragments arranged by size:
4543 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4958 5612 1937
Size: 3021 654 4594
Fragments arranged by size:
4594 3021 654
Acc65I G'GTAC_C
Cuts at: 5590 5590
Size: 8269
AclI AA'CG_TT
Cuts at: 1563 4844 6396 6769 1563
Size: 3281 1552 373 3063
Fragments arranged by size:
3281 3063 1552 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2520 4109 6332 7716 7984 1632
Size: 888 1589 2223 1384 268 1917
Fragments arranged by size:
2223 1917 1589 1384 888 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8072
AflIII A'CryG_T
Cuts at: 1203 3215 7168 8145 1203
Size: 2012 3953 977 1327
Fragments arranged by size:
3953 2012 1327 977
AgeI A'CCGG_T
Cuts at: 7100 7100
Size: 8269
AhdI GACnn_n'nnGTC
Cuts at: 4529 6998 4529
Size: 2469 5800
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8269
AlwNI CAG_nnn'CTG
Cuts at: 5092 6012 7584 5092
Size: 920 1572 5777
Fragments arranged by size:
5777 1572 920
ApaI G_GGCC'C
Cuts at: 5173 5173
Size: 8269
ApaLI G'TGCA_C
Cuts at: 6326 7482 6326
Size: 1156 7113
ApoI r'AATT_y
Cuts at: 1493 2440 3873 3989 4055 5578 5804 5956 8065
Size: 947 1433 116 66 1523 226 152 2109
Cuts at: 8065 1493
Size: 1697
Fragments arranged by size:
2109 1697 1523 1433 947 226 152 116
66
AseI AT'TA_AT
Cuts at: 160 1032 3800 4703 6823 160
Size: 872 2768 903 2120 1606
Fragments arranged by size:
2768 2120 1606 903 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8269
AvaI C'yCGr_G
Cuts at: 1870 1943 2508 2673 3351 5594 1870
Size: 73 565 165 678 2243 4545
Fragments arranged by size:
4545 2243 678 565 165 73
BamHI G'GATC_C
Cuts at: 2490 5599 5966 2490
Size: 3109 367 4793
Fragments arranged by size:
4793 3109 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5590 7045 618
Size: 325 217 627 3803 1455 1842
Fragments arranged by size:
3803 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4205 5173 5588 1391
Size: 2814 968 415 4072
Fragments arranged by size:
4072 2814 968 415
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2292 3514 4189 928
Size: 971 393 1222 675 5008
Fragments arranged by size:
5008 1222 971 675 393
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3081 3115 6460 6494 1903
Size: 34 50 34 1060 34 3345 34 3678
Fragments arranged by size:
3678 3345 1060 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2209 6161 7371 2209
Size: 3952 1210 3107
Fragments arranged by size:
3952 3107 1210
BclI T'GATC_A
Cuts at: 1548 2293 1548
Size: 745 7524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6880 136
Size: 107 122 71 631 605 5208 1525
Fragments arranged by size:
5208 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2231 2612 3826 4312 4715 8264 2231
Size: 381 1214 486 403 3549 2236
Fragments arranged by size:
3549 2236 1214 486 403 381
BlpI GC'TnA_GC
Cuts at: 5070 5686 5070
Size: 616 7653
Bme1580I G_kGCm'C
Cuts at: 1790 5173 6330 7486 1790
Size: 3383 1157 1156 2573
Fragments arranged by size:
3383 2573 1157 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8269
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4422 4770 6000
Size: 416 53 83 399 24 3005 348 1230
Cuts at: 6000 6958 442
Size: 958 1753
Fragments arranged by size:
3005 1753 1230 958 416 399 348 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3665 3957 4536 4636 4776 5171 6929 1239
Size: 2426 292 579 100 140 395 1758 2579
Fragments arranged by size:
2579 2426 1758 579 395 292 140 100
Bpu10I CC'TnA_GC
Cuts at: 2585 2682 2585
Size: 97 8172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2791 4652 6394 7259 7557 2791
Size: 1861 1742 865 298 3503
Fragments arranged by size:
3503 1861 1742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2896 4386 4752 6932 882
Size: 617 1397 1490 366 2180 2219
Fragments arranged by size:
2219 2180 1490 1397 617 366
BsaAI yAC'GTr
Cuts at: 493 1204 3216 6060 493
Size: 711 2012 2844 2702
Fragments arranged by size:
2844 2702 2012 711
BsaBI GATnn'nnATC
Cuts at: 2236 2617 3294 4606 8263 2236
Size: 381 677 1312 3657 2242
Fragments arranged by size:
3657 2242 1312 677 381
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5083 6076 6458 7093
Size: 53 83 186 936 3550 993 382 635
Cuts at: 7093 275
Size: 1451
Fragments arranged by size:
3550 1451 993 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4490 6702 7100 7374 7521 7932 4490
Size: 2212 398 274 147 411 4827
Fragments arranged by size:
4827 2212 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4611 4641 5485 5515 1178
Size: 30 165 30 3208 30 844 30 3932
Fragments arranged by size:
3932 3208 844 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2755 2990 3254 4374 4428 5493 1186
Size: 1569 235 264 1120 54 1065 3962
Fragments arranged by size:
3962 1569 1120 1065 264 235 54
BseYI C'CCAG_C
Cuts at: 7472 7968 7472
Size: 496 7773
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7696
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5719 6480 7508 1540
Size: 275 186 3718 761 1028 2301
Fragments arranged by size:
3718 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2515 4883 6330 6415 7486 2515
Size: 2368 1447 85 1071 3298
Fragments arranged by size:
3298 2368 1447 1071 85
BsmI GAATG_Cn'
Cuts at: 2335 4008 5780 5873 2335
Size: 1673 1772 93 4731
Fragments arranged by size:
4731 1772 1673 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2515 4205 4883 5173 5588 6330 6415
Size: 399 725 1690 678 290 415 742 85
Cuts at: 6415 7486 1391
Size: 1071 2174
Fragments arranged by size:
2174 1690 1071 742 725 678 415 399
290 85
BspEI T'CCGG_A
Cuts at: 7932 7932
Size: 8269
BspHI T'CATG_A
Cuts at: 4683 6158 7888 4683
Size: 1475 1730 5064
Fragments arranged by size:
5064 1730 1475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4741 5110 5624 844
Size: 3897 369 514 3489
Fragments arranged by size:
3897 3489 514 369
BsrBI CCG'CTC
Cuts at: 1394 1823 4636 4650 6156 1394
Size: 429 2813 14 1506 3507
Fragments arranged by size:
3507 2813 1506 429 14
BsrDI GCAATG_nn'
Cuts at: 65 1248 2018 2229 3372 4145 5112 6764 6938
Size: 1183 770 211 1143 773 967 1652 174
Cuts at: 6938 65
Size: 1396
Fragments arranged by size:
1652 1396 1183 1143 967 773 770 211
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6913 7100 1700
Size: 110 5103 187 2869
Fragments arranged by size:
5103 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3540 96
Size: 3444 4825
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8269
BssSI C'ACGA_G
Cuts at: 4032 6329 7341 4032
Size: 2297 1012 4960
Fragments arranged by size:
4960 2297 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5102 794
Size: 4308 3961
BstBI TT'CG_AA
Cuts at: 5322 7073 5322
Size: 1751 6518
BstEII G'GTnAC_C
Cuts at: 1184 3791 4422 1184
Size: 2607 631 5031
Fragments arranged by size:
5031 2607 631
BstYI r'GATC_y
Cuts at: 1972 2231 2490 2612 2809 3826 4312 4601 4655
Size: 259 259 122 197 1017 486 289 54
Cuts at: 4655 4715 5488 5599 5966 6351 6368 7820 7906
Size: 60 773 111 367 385 17 1452 86
Cuts at: 7906 7918 8264 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1017 773 486 385 367 346
289 259 259 197 122 111 86 60
54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2637 4192 4914 5173 513
Size: 552 1572 1555 722 259 3609
Fragments arranged by size:
3609 1572 1555 722 552 259
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7530
Fragments arranged by size:
7530 683 56
BtsI GCAGTG_nn'
Cuts at: 5204 5785 6579 6599 5204
Size: 581 794 20 6874
Fragments arranged by size:
6874 794 581 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8269
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8234
DraI TTT'AAA
Cuts at: 5573 5573
Size: 8269
DraIII CAC_nnn'GTG
Cuts at: 4430 4430
Size: 8269
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4305 7276 809
Size: 507 2989 2971 1802
Fragments arranged by size:
2989 2971 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3151 4911 5374
Size: 54 1425 50 275 18 1321 1760 463
Cuts at: 5374 5716 6605 8234 8
Size: 342 889 1629 43
Fragments arranged by size:
1760 1629 1425 1321 889 463 342 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5716 1537
Size: 275 3904 4090
Fragments arranged by size:
4090 3904 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2403 3159 3979 4147 5319 5509 6199 1353
Size: 1050 756 820 168 1172 190 690 3423
Fragments arranged by size:
3423 1172 1050 820 756 690 190 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6856 7230 7376 387
Size: 1064 138 5267 374 146 1280
Fragments arranged by size:
5267 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2520 3665 3957 4109 4536 4636 4776
Size: 393 888 1145 292 152 427 100 140
Cuts at: 4776 5171 6332 6929 7716 7984 1239
Size: 395 1161 597 787 268 1524
Fragments arranged by size:
1524 1161 1145 888 787 597 427 395
393 292 268 152 140 100
EcoNI CCTnn'n_nnAGG
Cuts at: 3236 4741 3236
Size: 1505 6764
EcoO109I rG'GnC_Cy
Cuts at: 4476 4476
Size: 8269
EcoRI G'AATT_C
Cuts at: 4055 5578 5956 4055
Size: 1523 378 6368
Fragments arranged by size:
6368 1523 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3599 3879 5535 3599
Size: 280 1656 6333
Fragments arranged by size:
6333 1656 280
FspI TGC'GCA
Cuts at: 6775 6775
Size: 8269
HaeII r_GCGC'y
Cuts at: 1678 7416 1678
Size: 5738 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 2402 2434 2585
Size: 32 135 32 671 32 127 32 151
Cuts at: 2585 2617 2801 2833 4515 4547 4735 4767 5375
Size: 32 184 32 1682 32 188 32 608
Cuts at: 5375 5407 6910 6942 6984 7016 1373
Size: 32 1503 32 42 32 2626
Fragments arranged by size:
2626 1682 1503 671 608 188 184 151
135 127 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5613 5859 669
Size: 988 281 3675 246 3079
Fragments arranged by size:
3675 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8269
HpaI GTT'AAC
Cuts at: 5859 5859
Size: 8269
KpnI G_GTAC'C
Cuts at: 5594 5594
Size: 8269
MfeI C'AATT_G
Cuts at: 5868 5868
Size: 8269
MluI A'CGCG_T
Cuts at: 8145 8145
Size: 8269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2340 3081 4430 4827 5516 7383 7567 1305
Size: 1035 741 1349 397 689 1867 184 2007
Fragments arranged by size:
2007 1867 1349 1035 741 689 397 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3153 10
Size: 54 1425 343 1321 5126
Fragments arranged by size:
5126 1425 1321 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2055 2535 6227 6586 6745 518
Size: 648 889 480 3692 359 159 2042
Fragments arranged by size:
3692 2042 889 648 480 359 159
MspA1I CmG'CkG
Cuts at: 1606 5149 5685 6362 7510 7755 1606
Size: 3543 536 677 1148 245 2120
Fragments arranged by size:
3543 2120 1148 677 536 245
NcoI C'CATG_G
Cuts at: 513 1065 2637 4192 513
Size: 552 1572 1555 4590
Fragments arranged by size:
4590 1572 1555 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8269
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8269
NotI GC'GGCC_GC
Cuts at: 5716 5716
Size: 8269
NspI r_CATG'y
Cuts at: 1132 1229 3638 5627 7172 1132
Size: 97 2409 1989 1545 2229
Fragments arranged by size:
2409 2229 1989 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8269
PciI A'CATG_T
Cuts at: 7168 7168
Size: 8269
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3160 5092 1108
Size: 2052 1932 4285
Fragments arranged by size:
4285 2052 1932
PfoI T'CCnGG_A
Cuts at: 2570 3718 5049 2570
Size: 1148 1331 5790
Fragments arranged by size:
5790 1331 1148
PmeI GTTT'AAAC
Cuts at: 5573 5573
Size: 8269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6338 6370 7851 7883 6338
Size: 32 1481 32 6724
Fragments arranged by size:
6724 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4476 4476
Size: 8269
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8269
PsiI TTA'TAA
Cuts at: 4500 5839 4500
Size: 1339 6930
PspOMI G'GGCC_C
Cuts at: 5169 5169
Size: 8269
PstI C_TGCA'G
Cuts at: 830 5621 830
Size: 4791 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8269
PvuII CAG'CTG
Cuts at: 5149 5149
Size: 8269
SalI G'TCGA_C
Cuts at: 1936 5611 1936
Size: 3675 4594
SanDI GG'GwC_CC
Cuts at: 4476 4476
Size: 8269
SapI GCTCTTCn'nnn_
Cuts at: 5509 5509
Size: 8269
SbfI CC_TGCA'GG
Cuts at: 5621 5621
Size: 8269
ScaI AGT'ACT
Cuts at: 4789 6517 8229 4789
Size: 1728 1712 4829
Fragments arranged by size:
4829 1728 1712
SexAI A'CCwGG_T
Cuts at: 3113 3478 5191 3113
Size: 365 1713 6191
Fragments arranged by size:
6191 1713 365
SfcI C'TryA_G
Cuts at: 826 1045 3462 4959 4981 5617 6752 7433 7624
Size: 219 2417 1497 22 636 1135 681 191
Cuts at: 7624 826
Size: 1471
Fragments arranged by size:
2417 1497 1471 1135 681 636 219 191
22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8269
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8269
SmaI CCC'GGG
Cuts at: 1872 5596 1872
Size: 3724 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2673 2770 3351 4631 6373 7274
Size: 197 926 730 97 581 1280 1742 901
Cuts at: 7274 7536 820
Size: 262 1553
Fragments arranged by size:
1742 1553 1280 926 901 730 581 262
197 97
SnaBI TAC'GTA
Cuts at: 493 6060 493
Size: 5567 2702
SpeI A'CTAG_T
Cuts at: 152 3080 152
Size: 2928 5341
SphI G_CATG'C
Cuts at: 1132 5627 1132
Size: 4495 3774
SspI AAT'ATT
Cuts at: 5 52 2195 6193 5
Size: 47 2143 3998 2081
Fragments arranged by size:
3998 2143 2081 47
StuI AGG'CCT
Cuts at: 1517 3598 4908 1517
Size: 2081 1310 4878
Fragments arranged by size:
4878 2081 1310
StyI C'CwwG_G
Cuts at: 513 1065 2637 3854 4192 5059 5496 5708 513
Size: 552 1572 1217 338 867 437 212 3074
Fragments arranged by size:
3074 1572 1217 867 552 437 338 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4441 5099 6296 6313 6466 6651 7096
Size: 1255 2861 658 1197 17 153 185 445
Cuts at: 7096 325
Size: 1498
Fragments arranged by size:
2861 1498 1255 1197 658 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3141 3540 4787
Size: 274 80 33 51 1443 1164 399 1247
Cuts at: 4787 4895 6515 8227 96
Size: 108 1620 1712 138
Fragments arranged by size:
1712 1620 1443 1247 1164 399 274 138
108 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4416 4448 4651 4683 8229 8261 4416
Size: 32 203 32 3546 32 4424
Fragments arranged by size:
4424 3546 203 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8269
XbaI T'CTAG_A
Cuts at: 3553 5605 3553
Size: 2052 6217
XhoI C'TCGA_G
Cuts at: 1943 2673 3351 1943
Size: 730 678 6861
Fragments arranged by size:
6861 730 678
XmaI C'CCGG_G
Cuts at: 1870 5594 1870
Size: 3724 4545
XmnI GAAnn'nnTTC
Cuts at: 5518 6398 5518
Size: 880 7389
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6077 276
Size: 53 83 186 936 4543 2468
Fragments arranged by size:
4543 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SfiI
SfoI SrfI SwaI XcmI