Restriction map for
pFN21AA0749
(Circular) (Six-base) MAPSORT of: pFN21AA0749.seq Check: 1769 from: 1 to: 6676
pFN21AA0749 6676 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2469 3253 2469
Size: 784 5892
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2685 4486 278
Size: 53 83 186 936 1149 1801 2468
Fragments arranged by size:
2468 1801 1149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2266 4019 1937
Size: 329 1753 4594
Fragments arranged by size:
4594 1753 329
Acc65I G'GTAC_C
Cuts at: 3997 3997
Size: 6676
AclI AA'CG_TT
Cuts at: 1563 4803 5176 1563
Size: 3240 373 3063
Fragments arranged by size:
3240 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4739 6123 6391 1632
Size: 3107 1384 268 1917
Fragments arranged by size:
3107 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6479
AflIII A'CryG_T
Cuts at: 1203 2051 3292 5575 6552 1203
Size: 848 1241 2283 977 1327
Fragments arranged by size:
2283 1327 1241 977 848
AgeI A'CCGG_T
Cuts at: 5507 5507
Size: 6676
AhdI GACnn_n'nnGTC
Cuts at: 2398 5405 2398
Size: 3007 3669
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6676
AlwNI CAG_nnn'CTG
Cuts at: 2025 3054 3178 3713 4419 5991 2025
Size: 1029 124 535 706 1572 2710
Fragments arranged by size:
2710 1572 1029 706 535 124
ApaI G_GGCC'C
Cuts at: 2145 2248 2418 2442 3660 3673 2145
Size: 103 170 24 1218 13 5148
Fragments arranged by size:
5148 1218 170 103 24 13
ApaLI G'TGCA_C
Cuts at: 4733 5889 4733
Size: 1156 5520
ApoI r'AATT_y
Cuts at: 1493 3985 4211 4363 6472 1493
Size: 2492 226 152 2109 1697
Fragments arranged by size:
2492 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3625 3625
Size: 6676
AseI AT'TA_AT
Cuts at: 160 1032 5230 160
Size: 872 4198 1606
Fragments arranged by size:
4198 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
AvaI C'yCGr_G
Cuts at: 1870 1943 2065 2163 2260 2295 2435 2579 2687
Size: 73 122 98 97 35 140 144 108
Cuts at: 2687 2953 3787 3821 4001 1870
Size: 266 834 34 180 4545
Fragments arranged by size:
4545 834 266 180 144 140 122 108
98 97 73 35 34
BamHI G'GATC_C
Cuts at: 2069 2662 2729 4006 4373 2069
Size: 593 67 1277 367 4372
Fragments arranged by size:
4372 1277 593 367 67
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2347 2593 2867 2937 3068
Size: 325 217 627 560 246 274 70 131
Cuts at: 3068 3791 3997 5452 618
Size: 723 206 1455 1842
Fragments arranged by size:
1842 1455 723 627 560 325 274 246
217 206 131 70
BanII G_rGCy'C
Cuts at: 1391 2032 2145 2164 2248 2258 2283 2319 2418
Size: 641 113 19 84 10 25 36 99
Cuts at: 2418 2442 2769 2954 3061 3495 3583 3660 3673
Size: 24 327 185 107 434 88 77 13
Cuts at: 3673 3744 3995 1391
Size: 71 251 4072
Fragments arranged by size:
4072 641 434 327 251 185 113 107
99 88 84 77 71 36 25 24
19 13 10
BbeI G_GCGC'C
Cuts at: 2351 3795 2351
Size: 1444 5232
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3863 928
Size: 971 1964 3741
Fragments arranged by size:
3741 1964 971
BbvCI CC'TCA_GC
Cuts at: 3435 3735 3435
Size: 300 6376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2761 2795 4867 4901 1903
Size: 34 824 34 2072 34 3678
Fragments arranged by size:
3678 2072 824 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3704 4568 5778 3704
Size: 864 1210 4602
Fragments arranged by size:
4602 1210 864
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6676
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2092 2309 2356
Size: 107 122 71 631 605 420 217 47
Cuts at: 2356 3426 5287 136
Size: 1070 1861 1525
Fragments arranged by size:
1861 1525 1070 631 605 420 217 122
107 71 47
BglII A'GATC_T
Cuts at: 2979 6671 2979
Size: 3692 2984
BlpI GC'TnA_GC
Cuts at: 3743 4093 3743
Size: 350 6326
Bme1580I G_kGCm'C
Cuts at: 1790 2145 2248 2418 2442 2598 2914 3151 3660
Size: 355 103 170 24 156 316 237 509
Cuts at: 3660 3673 4737 5893 1790
Size: 13 1064 1156 2573
Fragments arranged by size:
2573 1156 1064 509 355 316 237 170
156 103 24 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6676
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2035 4407 5365
Size: 416 53 83 399 24 618 2372 958
Cuts at: 5365 442
Size: 1753
Fragments arranged by size:
2372 1753 958 618 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6676
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3102 3102
Size: 6676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2089 2607 3143 3485 5336 1239
Size: 850 518 536 342 1851 2579
Fragments arranged by size:
2579 1851 850 536 518 342
Bpu10I CC'TnA_GC
Cuts at: 3435 3735 3435
Size: 300 6376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2930 4801 5666 5964 2930
Size: 1871 865 298 3642
Fragments arranged by size:
3642 1871 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2207 2705 2723 3110 5339 882
Size: 617 708 498 18 387 2229 2219
Fragments arranged by size:
2229 2219 708 617 498 387 18
BsaAI yAC'GTr
Cuts at: 493 1204 2052 2524 3216 4467 493
Size: 711 848 472 692 1251 2702
Fragments arranged by size:
2702 1251 848 711 692 472
BsaBI GATnn'nnATC
Cuts at: 6670 6670
Size: 6676
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2189 2348 2497 2682
Size: 53 83 186 936 656 159 149 185
Cuts at: 2682 3195 3210 3522 3792 4483 4865 5500 275
Size: 513 15 312 270 691 382 635 1451
Fragments arranged by size:
1451 936 691 656 635 513 382 312
270 186 185 159 149 83 53 15
BsaWI w'CCGG_w
Cuts at: 3071 3112 5109 5507 5781 5928 6339 3071
Size: 41 1997 398 274 147 411 3408
Fragments arranged by size:
3408 1997 411 398 274 147 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2887 2917 1178
Size: 30 165 30 1484 30 4937
Fragments arranged by size:
4937 1484 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3350 3482 3751 3752 3929 1186
Size: 2164 132 269 1 177 3933
Fragments arranged by size:
3933 2164 269 177 132 1
BseYI C'CCAG_C
Cuts at: 2304 2502 2611 3258 3311 3565 3639 5879 6375
Size: 198 109 647 53 254 74 2240 496
Cuts at: 6375 2304
Size: 2605
Fragments arranged by size:
2605 2240 647 496 254 198 109 74
53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3287 1163
Size: 573 1551 4552
Fragments arranged by size:
4552 1551 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2336 2513 3634 4126 4887 5915
Size: 275 186 335 177 1121 492 761 1028
Cuts at: 5915 1540
Size: 2301
Fragments arranged by size:
2301 1121 1028 761 492 335 275 186
177
BsiHKAI G_wGCw'C
Cuts at: 2164 3552 3744 4737 4822 5893 2164
Size: 1388 192 993 85 1071 2947
Fragments arranged by size:
2947 1388 1071 993 192 85
BsmI GAATG_Cn'
Cuts at: 4187 4280 4187
Size: 93 6583
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2145 2164 2248 2258 2283 2319
Size: 399 242 113 19 84 10 25 36
Cuts at: 2319 2418 2442 2598 2769 2914 2954 3061 3151
Size: 99 24 156 171 145 40 107 90
Cuts at: 3151 3495 3552 3583 3660 3673 3744 3995 4737
Size: 344 57 31 77 13 71 251 742
Cuts at: 4737 4822 5893 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 344 251 242 171
156 145 113 107 99 90 85 84
77 71 57 40 36 31 25 24
19 13 10
BspEI T'CCGG_A
Cuts at: 6339 6339
Size: 6676
BspHI T'CATG_A
Cuts at: 4565 6295 4565
Size: 1730 4946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2469 3253 3522 3964 4031 844
Size: 1625 784 269 442 67 3489
Fragments arranged by size:
3489 1625 784 442 269 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2200 2747 2924 4563 1394
Size: 429 377 547 177 1639 3507
Fragments arranged by size:
3507 1639 547 429 377 177
BsrDI GCAATG_nn'
Cuts at: 65 1248 5171 5345 65
Size: 1183 3923 174 1396
Fragments arranged by size:
3923 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2091 2508 3629 5320 5507 1700
Size: 110 281 417 1121 1691 187 2869
Fragments arranged by size:
2869 1691 1121 417 281 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6676
BssHII G'CGCG_C
Cuts at: 1925 3625 1925
Size: 1700 4976
BssSI C'ACGA_G
Cuts at: 4736 5748 4736
Size: 1012 5664
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3274 794
Size: 2480 4196
BstBI TT'CG_AA
Cuts at: 5480 5480
Size: 6676
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6676
BstYI r'GATC_y
Cuts at: 1972 2069 2662 2670 2729 2930 2979 3888 4006
Size: 97 593 8 59 201 49 909 118
Cuts at: 4006 4373 4758 4775 6227 6313 6325 6671 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 909 593 385 367 346 201
118 97 86 59 49 17 12 8
Bsu36I CC'TnA_GG
Cuts at: 3559 3559
Size: 6676
BtgI C'CryG_G
Cuts at: 513 1065 2004 3184 3351 3421 513
Size: 552 939 1180 167 70 3768
Fragments arranged by size:
3768 1180 939 552 167 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5937
Fragments arranged by size:
5937 683 56
BtsI GCAGTG_nn'
Cuts at: 4192 4986 5006 4192
Size: 794 20 5862
Fragments arranged by size:
5862 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6676
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2221 2256 529
Size: 35 1657 35 4949
Fragments arranged by size:
4949 1657 35 35
DraI TTT'AAA
Cuts at: 3980 3980
Size: 6676
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5683 809
Size: 507 4367 1802
Fragments arranged by size:
4367 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2333 2510
Size: 54 1425 50 275 18 318 185 177
Cuts at: 2510 2748 3631 4123 5012 6641 8
Size: 238 883 492 889 1629 43
Fragments arranged by size:
1629 1425 889 883 492 318 275 238
185 177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2333 2510 3631 4123 1537
Size: 275 521 177 1121 492 4090
Fragments arranged by size:
4090 1121 521 492 275 177
EarI CTCTTCn'nnn_
Cuts at: 1353 2648 3929 3962 4606 1353
Size: 1295 1281 33 644 3423
Fragments arranged by size:
3423 1295 1281 644 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3686 3817 5263 5637 5783 387
Size: 1064 138 2097 131 1446 374 146 1280
Fragments arranged by size:
2097 1446 1280 1064 374 146 138 131
EcoICRI GAG'CTC
Cuts at: 2162 3742 2162
Size: 1580 5096
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2089 2607 3143 3485 4739 5336 6123
Size: 393 457 518 536 342 1254 597 787
Cuts at: 6123 6391 1239
Size: 268 1524
Fragments arranged by size:
1524 1254 787 597 536 518 457 393
342 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2150 3821 2150
Size: 1671 5005
EcoO109I rG'GnC_Cy
Cuts at: 2141 2142 2244 2414 2415 2439 2546 2556 2576
Size: 1 102 170 1 24 107 10 20
Cuts at: 2576 3175 3656 3657 3669 3670 3813 3919 3948
Size: 599 481 1 12 1 143 106 29
Cuts at: 3948 2141
Size: 4869
Fragments arranged by size:
4869 599 481 170 143 107 106 102
29 24 20 12 10 1 1 1
1
EcoRI G'AATT_C
Cuts at: 3985 4363 3985
Size: 378 6298
FspI TGC'GCA
Cuts at: 5182 5182
Size: 6676
HaeII r_GCGC'y
Cuts at: 1678 2344 2351 2366 3795 5823 1678
Size: 666 7 15 1429 2028 2531
Fragments arranged by size:
2531 2028 1429 666 15 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2384 2416 2647 2679 2964
Size: 32 135 32 812 32 231 32 285
Cuts at: 2964 2996 5317 5349 5391 5423 1373
Size: 32 2321 32 42 32 2626
Fragments arranged by size:
2626 2321 812 285 231 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2267 4020 4266 669
Size: 988 281 329 1753 246 3079
Fragments arranged by size:
3079 1753 988 329 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6676
HpaI GTT'AAC
Cuts at: 4266 4266
Size: 6676
KasI G'GCGC_C
Cuts at: 2347 3791 2347
Size: 1444 5232
KpnI G_GTAC'C
Cuts at: 4001 4001
Size: 6676
MfeI C'AATT_G
Cuts at: 4275 4275
Size: 6676
MluI A'CGCG_T
Cuts at: 3292 6552 3292
Size: 3260 3416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2728 3110 3269 3582 3808 5790 5974 1305
Size: 1423 382 159 313 226 1982 184 2007
Fragments arranged by size:
2007 1982 1423 382 313 226 184 159
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4854
Fragments arranged by size:
4854 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4634 4993 5152 518
Size: 648 3468 359 159 2042
Fragments arranged by size:
3468 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2308 2502 2615 3423 3622 3863 4092 4769
Size: 702 194 113 808 199 241 229 677
Cuts at: 4769 5917 6162 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 808 702 677 245 241 229
199 194 113
NaeI GCC'GGC
Cuts at: 2093 3631 2093
Size: 1538 5138
NarI GG'CG_CC
Cuts at: 2348 3792 2348
Size: 1444 5232
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5185
Fragments arranged by size:
5185 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6676
NgoMIV G'CCGG_C
Cuts at: 2091 3629 2091
Size: 1538 5138
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6676
NotI GC'GGCC_GC
Cuts at: 4123 4123
Size: 6676
NspI r_CATG'y
Cuts at: 1132 1229 4034 5579 1132
Size: 97 2805 1545 2229
Fragments arranged by size:
2805 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2097 2396 2418 3054 3172 3583 3653 1348
Size: 749 299 22 636 118 411 70 4371
Fragments arranged by size:
4371 749 636 411 299 118 70 22
PciI A'CATG_T
Cuts at: 5575 5575
Size: 6676
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2025 2741 2997 3054 1108
Size: 917 716 256 57 4730
Fragments arranged by size:
4730 917 716 256 57
PfoI T'CCnGG_A
Cuts at: 3061 3061
Size: 6676
PmeI GTTT'AAAC
Cuts at: 3980 3980
Size: 6676
PmlI CAC'GTG
Cuts at: 2052 3216 2052
Size: 1164 5512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4745 4777 6258 6290 4745
Size: 32 1481 32 5131
Fragments arranged by size:
5131 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2556 3813 3919 3948 2556
Size: 1257 106 29 5284
Fragments arranged by size:
5284 1257 106 29
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6676
PsiI TTA'TAA
Cuts at: 4246 4246
Size: 6676
PspOMI G'GGCC_C
Cuts at: 2141 2244 2414 2438 3656 3669 2141
Size: 103 170 24 1218 13 5148
Fragments arranged by size:
5148 1218 170 103 24 13
PspXI vC'TCGA_Gb
Cuts at: 2953 2953
Size: 6676
PstI C_TGCA'G
Cuts at: 830 2118 4028 830
Size: 1288 1910 3478
Fragments arranged by size:
3478 1910 1288
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6676
PvuII CAG'CTG
Cuts at: 2308 2615 2308
Size: 307 6369
SacI G_AGCT'C
Cuts at: 2164 3744 2164
Size: 1580 5096
SacII CC_GC'GG
Cuts at: 3424 3424
Size: 6676
SalI G'TCGA_C
Cuts at: 1936 2265 4018 1936
Size: 329 1753 4594
Fragments arranged by size:
4594 1753 329
SanDI GG'GwC_CC
Cuts at: 3948 3948
Size: 6676
SbfI CC_TGCA'GG
Cuts at: 4028 4028
Size: 6676
ScaI AGT'ACT
Cuts at: 4924 6636 4924
Size: 1712 4964
SfcI C'TryA_G
Cuts at: 826 1045 2114 2853 4024 5159 5840 6031 826
Size: 219 1069 739 1171 1135 681 191 1471
Fragments arranged by size:
1471 1171 1135 1069 739 681 219 191
SfoI GGC'GCC
Cuts at: 2349 3793 2349
Size: 1444 5232
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6676
SmaI CCC'GGG
Cuts at: 1872 2067 2262 2437 2581 3789 4003 1872
Size: 195 195 175 144 1208 214 4545
Fragments arranged by size:
4545 1208 214 195 195 175 144
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2945 2953 4780 5681 5943 820
Size: 197 926 1002 8 1827 901 262 1553
Fragments arranged by size:
1827 1553 1002 926 901 262 197 8
SnaBI TAC'GTA
Cuts at: 493 4467 493
Size: 3974 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6676
SphI G_CATG'C
Cuts at: 1132 4034 1132
Size: 2902 3774
SrfI GCCC'GGGC
Cuts at: 2437 2437
Size: 6676
SspI AAT'ATT
Cuts at: 5 52 4600 5
Size: 47 4548 2081
Fragments arranged by size:
4548 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6676
StyI C'CwwG_G
Cuts at: 513 1065 2004 2208 2559 4115 513
Size: 552 939 204 351 1556 3074
Fragments arranged by size:
3074 1556 939 552 351 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2231 4703 4720 4873 5058 5503 325
Size: 1255 651 2472 17 153 185 445 1498
Fragments arranged by size:
2472 1498 1255 651 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4922 6634 96
Size: 274 80 33 51 1443 2945 1712 138
Fragments arranged by size:
2945 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3343 3375 6636 6668 3343
Size: 32 3261 32 3351
Fragments arranged by size:
3351 3261 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3878 1316
Size: 2562 4114
XbaI T'CTAG_A
Cuts at: 4012 4012
Size: 6676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2403 2499 2403
Size: 96 6580
XhoI C'TCGA_G
Cuts at: 1943 2953 1943
Size: 1010 5666
XmaI C'CCGG_G
Cuts at: 1870 2065 2260 2435 2579 3787 4001 1870
Size: 195 195 175 144 1208 214 4545
Fragments arranged by size:
4545 1208 214 195 195 175 144
XmnI GAAnn'nnTTC
Cuts at: 3835 4805 3835
Size: 970 5706
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2683 4484 276
Size: 53 83 186 936 1149 1801 2468
Fragments arranged by size:
2468 1801 1149 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PsrI RsrII SapI SexAI SfiI SwaI