Restriction map for
pFN21AA0746
(Circular) (Six-base) MAPSORT of: pFN21AA0746.seq Check: 9896 from: 1 to: 7642
pFN21AA0746 7642 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5452 278
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4985 1937
Size: 3048 4594
Acc65I G'GTAC_C
Cuts at: 4963 4963
Size: 7642
AclI AA'CG_TT
Cuts at: 1563 5769 6142 1563
Size: 4206 373 3063
Fragments arranged by size:
4206 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2819 4642 5705 7089 7357 1632
Size: 1187 1823 1063 1384 268 1917
Fragments arranged by size:
1917 1823 1384 1187 1063 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7445
AflIII A'CryG_T
Cuts at: 1203 4141 6541 7518 1203
Size: 2938 2400 977 1327
Fragments arranged by size:
2938 2400 1327 977
AgeI A'CCGG_T
Cuts at: 6473 6473
Size: 7642
AhdI GACnn_n'nnGTC
Cuts at: 6371 6371
Size: 7642
AleI CACnn'nnGTG
Cuts at: 1166 4290 1166
Size: 3124 4518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7642
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3285 3317 3285
Size: 32 7610
AlwNI CAG_nnn'CTG
Cuts at: 2100 3243 3640 4253 4618 4665 4847 5385 6957
Size: 1143 397 613 365 47 182 538 1572
Cuts at: 6957 2100
Size: 2785
Fragments arranged by size:
2785 1572 1143 613 538 397 365 182
47
ApaLI G'TGCA_C
Cuts at: 5699 6855 5699
Size: 1156 6486
ApoI r'AATT_y
Cuts at: 1493 2585 3869 4276 4951 5177 5329 7438 1493
Size: 1092 1284 407 675 226 152 2109 1697
Fragments arranged by size:
2109 1697 1284 1092 675 407 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6196 160
Size: 872 5164 1606
Fragments arranged by size:
5164 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
AvaI C'yCGr_G
Cuts at: 1870 1943 2347 3306 3600 4967 1870
Size: 73 404 959 294 1367 4545
Fragments arranged by size:
4545 1367 959 404 294 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4312 4345 4312
Size: 33 7609
BamHI G'GATC_C
Cuts at: 3721 4972 5339 3721
Size: 1251 367 6024
Fragments arranged by size:
6024 1251 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3704 4963 6418 618
Size: 325 217 627 1917 1259 1455 1842
Fragments arranged by size:
1917 1842 1455 1259 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4961 1391
Size: 3570 4072
BbeI G_GCGC'C
Cuts at: 3708 3708
Size: 7642
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2571 3188 3360 928
Size: 971 672 617 172 5210
Fragments arranged by size:
5210 971 672 617 172
BbvCI CC'TCA_GC
Cuts at: 2901 2901
Size: 7642
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5833 5867 1903
Size: 34 3896 34 3678
Fragments arranged by size:
3896 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3242 5534 6744 3242
Size: 2292 1210 4140
Fragments arranged by size:
4140 2292 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7642
BfrBI ATG'CAT
Cuts at: 2548 2930 2548
Size: 382 7260
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6253 136
Size: 107 122 71 631 605 4581 1525
Fragments arranged by size:
4581 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7637 7637
Size: 7642
BlpI GC'TnA_GC
Cuts at: 3618 5059 3618
Size: 1441 6201
Bme1580I G_kGCm'C
Cuts at: 1790 5703 6859 1790
Size: 3913 1156 2573
Fragments arranged by size:
3913 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 4854 1318
Size: 3536 4106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2396 3433 5373
Size: 416 53 83 399 24 979 1037 1940
Cuts at: 5373 6331 442
Size: 958 1753
Fragments arranged by size:
1940 1753 1037 979 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2296 2390 2981 3110 3731 4002 4115 4469
Size: 1057 94 591 129 621 271 113 354
Cuts at: 4469 4595 4654 4863 6302 1239
Size: 126 59 209 1439 2579
Fragments arranged by size:
2579 1439 1057 621 591 354 271 209
129 126 113 94 59
Bpu10I CC'TnA_GC
Cuts at: 2901 2901
Size: 7642
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2231 2873 3527 5767 6632 6930 2231
Size: 642 654 2240 865 298 2943
Fragments arranged by size:
2943 2240 865 654 642 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3007 4401 6305 882
Size: 617 1508 1394 1904 2219
Fragments arranged by size:
2219 1904 1508 1394 617
BsaAI yAC'GTr
Cuts at: 493 1204 4937 5433 493
Size: 711 3733 496 2702
Fragments arranged by size:
3733 2702 711 496
BsaBI GATnn'nnATC
Cuts at: 7636 7636
Size: 7642
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2383 3705 5449 5831
Size: 53 83 186 936 850 1322 1744 382
Cuts at: 5831 6466 275
Size: 635 1451
Fragments arranged by size:
1744 1451 1322 936 850 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6075 6473 6747 6894 7305 6075
Size: 398 274 147 411 6412
Fragments arranged by size:
6412 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4627 4657 1178
Size: 30 165 30 3224 30 4163
Fragments arranged by size:
4163 3224 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4310 4664 1186
Size: 3124 354 4164
Fragments arranged by size:
4164 3124 354
BseYI C'CCAG_C
Cuts at: 2735 3068 6845 7341 2735
Size: 333 3777 496 3036
Fragments arranged by size:
3777 3036 496 333
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2807 3046 4481 4673 4703 4802 1163
Size: 573 1071 239 1435 192 30 99 4003
Fragments arranged by size:
4003 1435 1071 573 239 192 99 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5092 5853 6881 1540
Size: 275 186 3091 761 1028 2301
Fragments arranged by size:
3091 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5703 5788 6859 5703
Size: 85 1071 6486
Fragments arranged by size:
6486 1071 85
BsmI GAATG_Cn'
Cuts at: 5153 5246 5153
Size: 93 7549
BsmBI CGTCTCn'nnnn_
Cuts at: 3707 3707
Size: 7642
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4961 5703 5788 6859 1391
Size: 399 3171 742 85 1071 2174
Fragments arranged by size:
3171 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7305 7305
Size: 7642
BspHI T'CATG_A
Cuts at: 5531 7261 5531
Size: 1730 5912
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2751 2994 4695 4997 844
Size: 1907 243 1701 302 3489
Fragments arranged by size:
3489 1907 1701 302 243
BsrBI CCG'CTC
Cuts at: 1394 1823 5529 1394
Size: 429 3706 3507
Fragments arranged by size:
3706 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3083 3453 3613 6137 6311 65
Size: 1183 1835 370 160 2524 174 1396
Fragments arranged by size:
2524 1835 1396 1183 370 174 160
BsrFI r'CCGG_y
Cuts at: 1700 1810 6286 6473 1700
Size: 110 4476 187 2869
Fragments arranged by size:
4476 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7642
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7642
BssSI C'ACGA_G
Cuts at: 5702 6714 5702
Size: 1012 6630
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3487 794
Size: 2693 4949
BstBI TT'CG_AA
Cuts at: 6446 6446
Size: 7642
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7642
BstYI r'GATC_y
Cuts at: 1972 3097 3721 4111 4972 5339 5724 5741 7193
Size: 1125 624 390 861 367 385 17 1452
Cuts at: 7193 7279 7291 7637 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1125 861 624 390 385 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6151
Fragments arranged by size:
6151 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6903
Fragments arranged by size:
6903 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 3485 4845 4857 5158 5952 5972 2061
Size: 1424 1360 12 301 794 20 3731
Fragments arranged by size:
3731 1424 1360 794 301 20 12
ClaI AT'CG_AT
Cuts at: 1554 4374 1554
Size: 2820 4822
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2071 2106 2440 2475 2644 2679 529
Size: 35 1507 35 334 35 169 35 5492
Fragments arranged by size:
5492 1507 334 169 35 35 35 35
DraI TTT'AAA
Cuts at: 4946 4946
Size: 7642
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6649 809
Size: 507 5333 1802
Fragments arranged by size:
5333 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3663 4311 4423
Size: 54 1425 50 275 18 1833 648 112
Cuts at: 4423 5089 5978 7607 8
Size: 666 889 1629 43
Fragments arranged by size:
1833 1629 1425 889 666 648 275 112
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5089 1537
Size: 275 3277 4090
Fragments arranged by size:
4090 3277 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2517 5572 1353
Size: 1164 3055 3423
Fragments arranged by size:
3423 3055 1164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6229 6603 6749 387
Size: 1064 138 4640 374 146 1280
Fragments arranged by size:
4640 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2296 2390 2819 2981 3110 3731 4002
Size: 393 664 94 429 162 129 621 271
Cuts at: 4002 4115 4469 4595 4642 4654 4863 5705 6302
Size: 113 354 126 47 12 209 842 597
Cuts at: 6302 7089 7357 1239
Size: 787 268 1524
Fragments arranged by size:
1524 842 787 664 621 597 429 393
354 271 268 209 162 129 126 113
94 47 12
EcoO109I rG'GnC_Cy
Cuts at: 4056 4056
Size: 7642
EcoRI G'AATT_C
Cuts at: 4951 5329 4951
Size: 378 7264
EcoRV GAT'ATC
Cuts at: 2623 2623
Size: 7642
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2225 2225
Size: 7642
FspI TGC'GCA
Cuts at: 4035 6148 4035
Size: 2113 5529
HaeII r_GCGC'y
Cuts at: 1678 3708 6789 1678
Size: 2030 3081 2531
Fragments arranged by size:
3081 2531 2030
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2025 2057 2607 2639 2827
Size: 32 135 32 453 32 550 32 188
Cuts at: 2827 2859 3350 3382 3706 3738 4478 4510 6283
Size: 32 491 32 324 32 740 32 1773
Cuts at: 6283 6315 6357 6389 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1773 740 550 491 453 324 188
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2479 4333 4986 5232 669
Size: 988 281 541 1854 653 246 3079
Fragments arranged by size:
3079 1854 988 653 541 281 246
HindIII A'AGCT_T
Cuts at: 748 4221 748
Size: 3473 4169
HpaI GTT'AAC
Cuts at: 4333 5232 4333
Size: 899 6743
KasI G'GCGC_C
Cuts at: 3704 3704
Size: 7642
KpnI G_GTAC'C
Cuts at: 4967 4967
Size: 7642
MfeI C'AATT_G
Cuts at: 3684 5241 3684
Size: 1557 6085
MluI A'CGCG_T
Cuts at: 7518 7518
Size: 7642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3322 6756 6940 1305
Size: 2017 3434 184 2007
Fragments arranged by size:
3434 2017 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3665 4313 4425 10
Size: 54 1425 343 1833 648 112 3227
Fragments arranged by size:
3227 1833 1425 648 343 112 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2724 3966 4290 5600 5959 6118 518
Size: 648 1558 1242 324 1310 359 159 2042
Fragments arranged by size:
2042 1558 1310 1242 648 359 324 159
MspA1I CmG'CkG
Cuts at: 1606 2735 3240 3316 4871 4883 5058 5735 6883
Size: 1129 505 76 1555 12 175 677 1148
Cuts at: 6883 7128 1606
Size: 245 2120
Fragments arranged by size:
2120 1555 1148 1129 677 505 245 175
76 12
NarI GG'CG_CC
Cuts at: 3705 3705
Size: 7642
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6151
Fragments arranged by size:
6151 939 552
NdeI CA'TA_TG
Cuts at: 387 3505 387
Size: 3118 4524
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7642
NotI GC'GGCC_GC
Cuts at: 5089 5089
Size: 7642
NsiI A_TGCA'T
Cuts at: 2550 2932 2550
Size: 382 7260
NspI r_CATG'y
Cuts at: 1132 1229 2970 3328 4145 5000 6545 1132
Size: 97 1741 358 817 855 1545 2229
Fragments arranged by size:
2229 1741 1545 855 817 358 97
PasI CC'CwG_GG
Cuts at: 1348 3039 4498 1348
Size: 1691 1459 4492
Fragments arranged by size:
4492 1691 1459
PciI A'CATG_T
Cuts at: 4141 6541 4141
Size: 2400 5242
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2392 4558 1108
Size: 1284 2166 4192
Fragments arranged by size:
4192 2166 1284
PfoI T'CCnGG_A
Cuts at: 2706 3988 4611 2706
Size: 1282 623 5737
Fragments arranged by size:
5737 1282 623
PmeI GTTT'AAAC
Cuts at: 4946 4946
Size: 7642
PmlI CAC'GTG
Cuts at: 4937 4937
Size: 7642
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5711 5743 7224 7256 5711
Size: 32 1481 32 6097
Fragments arranged by size:
6097 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4056 4056
Size: 7642
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7642
PsiI TTA'TAA
Cuts at: 2724 5212 2724
Size: 2488 5154
PstI C_TGCA'G
Cuts at: 830 2056 2163 2938 3321 4888 4994 830
Size: 1226 107 775 383 1567 106 3478
Fragments arranged by size:
3478 1567 1226 775 383 107 106
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7642
PvuII CAG'CTG
Cuts at: 2735 3240 3316 4871 4883 2735
Size: 505 76 1555 12 5494
Fragments arranged by size:
5494 1555 505 76 12
SalI G'TCGA_C
Cuts at: 1936 4984 1936
Size: 3048 4594
SbfI CC_TGCA'GG
Cuts at: 4994 4994
Size: 7642
ScaI AGT'ACT
Cuts at: 2804 3902 5890 7602 2804
Size: 1098 1988 1712 2844
Fragments arranged by size:
2844 1988 1712 1098
SfcI C'TryA_G
Cuts at: 826 1045 2052 2136 2159 2934 3317 3445 4884
Size: 219 1007 84 23 775 383 128 1439
Cuts at: 4884 4990 6125 6806 6997 826
Size: 106 1135 681 191 1471
Fragments arranged by size:
1471 1439 1135 1007 775 681 383 219
191 128 106 84 23
SfoI GGC'GCC
Cuts at: 3706 3706
Size: 7642
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7642
SmaI CCC'GGG
Cuts at: 1872 4969 1872
Size: 3097 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2210 2852 3542 5746 6647 6909
Size: 197 926 267 642 690 2204 901 262
Cuts at: 6909 820
Size: 1553
Fragments arranged by size:
2204 1553 926 901 690 642 267 262
197
SnaBI TAC'GTA
Cuts at: 493 5433 493
Size: 4940 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7642
SphI G_CATG'C
Cuts at: 1132 2970 5000 1132
Size: 1838 2030 3774
Fragments arranged by size:
3774 2030 1838
SspI AAT'ATT
Cuts at: 5 52 4019 5566 5
Size: 47 3967 1547 2081
Fragments arranged by size:
3967 2081 1547 47
StuI AGG'CCT
Cuts at: 1517 4188 4835 1517
Size: 2671 647 4324
Fragments arranged by size:
4324 2671 647
StyI C'CwwG_G
Cuts at: 513 1065 2004 3113 3699 3822 4671 5081 513
Size: 552 939 1109 586 123 849 410 3074
Fragments arranged by size:
3074 1109 939 849 586 552 410 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4316 5669 5686 5839 6024 6469 325
Size: 1255 2736 1353 17 153 185 445 1498
Fragments arranged by size:
2736 1498 1353 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2469 2802 3900
Size: 274 80 33 51 1443 492 333 1098
Cuts at: 3900 5888 7600 96
Size: 1988 1712 138
Fragments arranged by size:
1988 1712 1443 1098 492 333 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4248 4280 4849 4881 7602 7634 4248
Size: 32 569 32 2721 32 4256
Fragments arranged by size:
4256 2721 569 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7642
XbaI T'CTAG_A
Cuts at: 4978 4978
Size: 7642
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3005 3706 4321 3005
Size: 701 615 6326
Fragments arranged by size:
6326 701 615
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7642
XmaI C'CCGG_G
Cuts at: 1870 4967 1870
Size: 3097 4545
XmnI GAAnn'nnTTC
Cuts at: 5771 5771
Size: 7642
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5450 276
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BplI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI NaeI
NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI