Restriction map for
pFN21AA0740
(Circular) (Six-base) MAPSORT of: pFN21AA0740.seq Check: 8104 from: 1 to: 6793
pFN21AA0740 6793 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2652 2652
Size: 6793
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4603 278
Size: 53 83 186 936 3067 2468
Fragments arranged by size:
3067 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2985 4136 1937
Size: 1048 1151 4594
Fragments arranged by size:
4594 1151 1048
Acc65I G'GTAC_C
Cuts at: 3969 4114 3969
Size: 145 6648
AclI AA'CG_TT
Cuts at: 1563 2037 3452 4920 5293 1563
Size: 474 1415 1468 373 3063
Fragments arranged by size:
3063 1468 1415 474 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2675 2804 3201 3995 4856 6240 6508 1632
Size: 1043 129 397 794 861 1384 268 1917
Fragments arranged by size:
1917 1384 1043 861 794 397 268 129
AfeI AGC'GCT
Cuts at: 2625 2625
Size: 6793
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6596
AflIII A'CryG_T
Cuts at: 1203 5692 6669 1203
Size: 4489 977 1327
Fragments arranged by size:
4489 1327 977
AgeI A'CCGG_T
Cuts at: 2725 5624 2725
Size: 2899 3894
AhdI GACnn_n'nnGTC
Cuts at: 5522 5522
Size: 6793
AleI CACnn'nnGTG
Cuts at: 1166 2150 1166
Size: 984 5809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2660 1184
Size: 1476 5317
AlwNI CAG_nnn'CTG
Cuts at: 2140 3101 4536 6108 2140
Size: 961 1435 1572 2825
Fragments arranged by size:
2825 1572 1435 961
ApaI G_GGCC'C
Cuts at: 3014 3192 3014
Size: 178 6615
ApaLI G'TGCA_C
Cuts at: 4850 6006 4850
Size: 1156 5637
ApoI r'AATT_y
Cuts at: 1493 2736 2850 2876 4072 4102 4328 4480 6589
Size: 1243 114 26 1196 30 226 152 2109
Cuts at: 6589 1493
Size: 1697
Fragments arranged by size:
2109 1697 1243 1196 226 152 114 30
26
AseI AT'TA_AT
Cuts at: 160 1032 3680 5347 160
Size: 872 2648 1667 1606
Fragments arranged by size:
2648 1667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6793
AvaI C'yCGr_G
Cuts at: 1870 1943 2211 3323 4118 1870
Size: 73 268 1112 795 4545
Fragments arranged by size:
4545 1112 795 268 73
AvrII C'CTAG_G
Cuts at: 3017 3017
Size: 6793
BamHI G'GATC_C
Cuts at: 4123 4490 4123
Size: 367 6426
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2421 2472 2640 2695 3948
Size: 325 217 627 634 51 168 55 1253
Cuts at: 3948 3969 4114 5569 618
Size: 21 145 1455 1842
Fragments arranged by size:
1842 1455 1253 634 627 325 217 168
145 55 51 21
BanII G_rGCy'C
Cuts at: 1391 3014 3192 4112 1391
Size: 1623 178 920 4072
Fragments arranged by size:
4072 1623 920 178
BbeI G_GCGC'C
Cuts at: 2476 2476
Size: 6793
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2090 3039 928
Size: 971 191 949 4682
Fragments arranged by size:
4682 971 949 191
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4044 4078 4984 5018 1903
Size: 34 2107 34 906 34 3678
Fragments arranged by size:
3678 2107 906 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2248 2382 4685 5895 2248
Size: 134 2303 1210 3146
Fragments arranged by size:
3146 2303 1210 134
BclI T'GATC_A
Cuts at: 1548 2266 1548
Size: 718 6075
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5404 136
Size: 107 122 71 631 605 3732 1525
Fragments arranged by size:
3732 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6788 6788
Size: 6793
BlpI GC'TnA_GC
Cuts at: 4210 4210
Size: 6793
Bme1580I G_kGCm'C
Cuts at: 1790 3014 3192 3499 4854 6010 1790
Size: 1224 178 307 1355 1156 2573
Fragments arranged by size:
2573 1355 1224 1156 307 178
BmgBI CAC'GTC
Cuts at: 1318 3463 1318
Size: 2145 4648
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4524 5482 442
Size: 416 53 83 399 24 3107 958 1753
Fragments arranged by size:
3107 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6793
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2256 3812 2256
Size: 1556 5237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3233 4065 5453 1239
Size: 1994 832 1388 2579
Fragments arranged by size:
2579 1994 1388 832
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4033 4918 5783 6081 4033
Size: 885 865 298 4745
Fragments arranged by size:
4745 885 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5456 882
Size: 617 3957 2219
Fragments arranged by size:
3957 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2152 3409 4584 493
Size: 711 948 1257 1175 2702
Fragments arranged by size:
2702 1257 1175 948 711
BsaBI GATnn'nnATC
Cuts at: 6787 6787
Size: 6793
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2473 4600 4982 5617
Size: 53 83 186 936 940 2127 382 635
Cuts at: 5617 275
Size: 1451
Fragments arranged by size:
2127 1451 940 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2725 3119 5226 5624 5898 6045 6456 2725
Size: 394 2107 398 274 147 411 3062
Fragments arranged by size:
3062 2107 411 398 394 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6568
Fragments arranged by size:
6568 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3020 1186
Size: 1834 4959
BseYI C'CCAG_C
Cuts at: 5996 6492 5996
Size: 496 6297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3114 3850 1163
Size: 573 1378 736 4106
Fragments arranged by size:
4106 1378 736 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4243 5004 6032 1540
Size: 275 186 2242 761 1028 2301
Fragments arranged by size:
2301 2242 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3798 4854 4939 6010 3798
Size: 1056 85 1071 4581
Fragments arranged by size:
4581 1071 1056 85
BsmI GAATG_Cn'
Cuts at: 4304 4397 4304
Size: 93 6700
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3014 3192 3499 3798 4112 4854 4939
Size: 399 1224 178 307 299 314 742 85
Cuts at: 4939 6010 1391
Size: 1071 2174
Fragments arranged by size:
2174 1224 1071 742 399 314 307 299
178 85
BspEI T'CCGG_A
Cuts at: 6456 6456
Size: 6793
BspHI T'CATG_A
Cuts at: 3360 3378 4682 6412 3360
Size: 18 1304 1730 3741
Fragments arranged by size:
3741 1730 1304 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2289 2652 4148 844
Size: 1445 363 1496 3489
Fragments arranged by size:
3489 1496 1445 363
BsrBI CCG'CTC
Cuts at: 1394 1823 3946 4680 1394
Size: 429 2123 734 3507
Fragments arranged by size:
3507 2123 734 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5288 5462 65
Size: 1183 4040 174 1396
Fragments arranged by size:
4040 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2725 5437 5624 1700
Size: 110 915 2712 187 2869
Fragments arranged by size:
2869 2712 915 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6793
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6793
BssSI C'ACGA_G
Cuts at: 3766 4853 5865 3766
Size: 1087 1012 4694
Fragments arranged by size:
4694 1087 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6793
BstBI TT'CG_AA
Cuts at: 5597 5597
Size: 6793
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6793
BstXI CCAn_nnnn'nTGG
Cuts at: 2163 3199 2163
Size: 1036 5757
BstYI r'GATC_y
Cuts at: 1972 3661 4123 4490 4875 4892 6344 6430 6442
Size: 1689 462 367 385 17 1452 86 12
Cuts at: 6442 6788 1972
Size: 346 1977
Fragments arranged by size:
1977 1689 1452 462 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3027 3027
Size: 6793
BtgI C'CryG_G
Cuts at: 513 1065 2004 2076 2754 513
Size: 552 939 72 678 4552
Fragments arranged by size:
4552 939 678 552 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4028 487
Size: 683 56 2802 3252
Fragments arranged by size:
3252 2802 683 56
BtsI GCAGTG_nn'
Cuts at: 4092 4309 5103 5123 4092
Size: 217 794 20 5762
Fragments arranged by size:
5762 794 217 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6793
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6758
DraI TTT'AAA
Cuts at: 3472 4097 3472
Size: 625 6168
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3218 5800 809
Size: 507 1902 2582 1802
Fragments arranged by size:
2582 1902 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 3840 4089
Size: 54 1425 50 275 18 312 1698 249
Cuts at: 4089 4240 5129 6758 8
Size: 151 889 1629 43
Fragments arranged by size:
1698 1629 1425 889 312 275 249 151
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4240 1537
Size: 275 2428 4090
Fragments arranged by size:
4090 2428 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2872 4723 1353
Size: 1519 1851 3423
Fragments arranged by size:
3423 1851 1519
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2986 5380 5754 5900 387
Size: 1064 138 1397 2394 374 146 1280
Fragments arranged by size:
2394 1397 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2675 2804 3201 3233 3995 4065 4856
Size: 393 1043 129 397 32 762 70 791
Cuts at: 4856 5453 6240 6508 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1043 791 787 762 597 397 393
268 129 70 32
EcoNI CCTnn'n_nnAGG
Cuts at: 3065 3065
Size: 6793
EcoO109I rG'GnC_Cy
Cuts at: 3010 3188 3230 3319 3010
Size: 178 42 89 6484
Fragments arranged by size:
6484 178 89 42
EcoRI G'AATT_C
Cuts at: 4072 4102 4480 4072
Size: 30 378 6385
Fragments arranged by size:
6385 378 30
EcoRV GAT'ATC
Cuts at: 3606 3606
Size: 6793
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2107 3391 4081 2107
Size: 1284 690 4819
Fragments arranged by size:
4819 1284 690
FspI TGC'GCA
Cuts at: 5299 5299
Size: 6793
HaeII r_GCGC'y
Cuts at: 1678 2476 2627 5940 1678
Size: 798 151 3313 2531
Fragments arranged by size:
3313 2531 798 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2320 2352 5434 5466 5508
Size: 32 135 32 748 32 3082 32 42
Cuts at: 5508 5540 1373
Size: 32 2626
Fragments arranged by size:
3082 2626 748 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2986 3166 3753 4137 4383 669
Size: 988 281 1048 180 587 384 246 3079
Fragments arranged by size:
3079 1048 988 587 384 281 246 180
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6793
HpaI GTT'AAC
Cuts at: 4383 4383
Size: 6793
KasI G'GCGC_C
Cuts at: 2472 2472
Size: 6793
KpnI G_GTAC'C
Cuts at: 3973 4118 3973
Size: 145 6648
MfeI C'AATT_G
Cuts at: 4392 4392
Size: 6793
MluI A'CGCG_T
Cuts at: 6669 6669
Size: 6793
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5907 6091 1305
Size: 4602 184 2007
Fragments arranged by size:
4602 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 10
Size: 54 1425 343 312 4659
Fragments arranged by size:
4659 1425 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 3143 3197 4751 5110 5269 518
Size: 648 984 993 54 1554 359 159 2042
Fragments arranged by size:
2042 1554 993 984 648 359 159 54
MspA1I CmG'CkG
Cuts at: 1606 2137 3508 3673 3954 4209 4886 6034 6279
Size: 531 1371 165 281 255 677 1148 245
Cuts at: 6279 1606
Size: 2120
Fragments arranged by size:
2120 1371 1148 677 531 281 255 245
165
NarI GG'CG_CC
Cuts at: 2473 2473
Size: 6793
NcoI C'CATG_G
Cuts at: 513 1065 2004 2754 513
Size: 552 939 750 4552
Fragments arranged by size:
4552 939 750 552
NdeI CA'TA_TG
Cuts at: 387 2293 387
Size: 1906 4887
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6793
NotI GC'GGCC_GC
Cuts at: 4240 4240
Size: 6793
NspI r_CATG'y
Cuts at: 1132 1229 2368 3148 3740 4151 5696 1132
Size: 97 1139 780 592 411 1545 2229
Fragments arranged by size:
2229 1545 1139 780 592 411 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6793
PciI A'CATG_T
Cuts at: 5692 5692
Size: 6793
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3838 1108
Size: 2730 4063
PmeI GTTT'AAAC
Cuts at: 4097 4097
Size: 6793
PmlI CAC'GTG
Cuts at: 2152 2152
Size: 6793
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4862 4894 6375 6407 4862
Size: 32 1481 32 5248
Fragments arranged by size:
5248 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3319 3319
Size: 6793
PshAI GACnn'nnGTC
Cuts at: 1613 3200 1613
Size: 1587 5206
PsiI TTA'TAA
Cuts at: 4363 4363
Size: 6793
PspOMI G'GGCC_C
Cuts at: 3010 3188 3010
Size: 178 6615
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2535 2567 2535
Size: 32 6761
PstI C_TGCA'G
Cuts at: 830 3925 4145 830
Size: 3095 220 3478
Fragments arranged by size:
3478 3095 220
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6793
PvuII CAG'CTG
Cuts at: 2137 2137
Size: 6793
SalI G'TCGA_C
Cuts at: 1936 2984 4135 1936
Size: 1048 1151 4594
Fragments arranged by size:
4594 1151 1048
SbfI CC_TGCA'GG
Cuts at: 4145 4145
Size: 6793
ScaI AGT'ACT
Cuts at: 5041 6753 5041
Size: 1712 5081
SfcI C'TryA_G
Cuts at: 826 1045 3517 3921 4141 5276 5957 6148 826
Size: 219 2472 404 220 1135 681 191 1471
Fragments arranged by size:
2472 1471 1135 681 404 220 219 191
SfoI GGC'GCC
Cuts at: 2474 2474
Size: 6793
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6793
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6793
SmaI CCC'GGG
Cuts at: 1872 4120 1872
Size: 2248 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3323 4048 4897 5798 6060 820
Size: 197 926 1380 725 849 901 262 1553
Fragments arranged by size:
1553 1380 926 901 849 725 262 197
SnaBI TAC'GTA
Cuts at: 493 4584 493
Size: 4091 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6793
SphI G_CATG'C
Cuts at: 1132 3148 3740 4151 1132
Size: 2016 592 411 3774
Fragments arranged by size:
3774 2016 592 411
SspI AAT'ATT
Cuts at: 5 52 4717 5
Size: 47 4665 2081
Fragments arranged by size:
4665 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6793
StyI C'CwwG_G
Cuts at: 513 1065 2004 2754 3017 3687 4232 513
Size: 552 939 750 263 670 545 3074
Fragments arranged by size:
3074 939 750 670 552 545 263
SwaI ATTT'AAAT
Cuts at: 3472 3472
Size: 6793
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2054 2162 2409 4820 4837 4990 5175
Size: 1255 474 108 247 2411 17 153 185
Cuts at: 5175 5620 325
Size: 445 1498
Fragments arranged by size:
2411 1498 1255 474 445 247 185 153
108 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5039 6751 96
Size: 274 80 33 51 1443 3062 1712 138
Fragments arranged by size:
3062 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2394 2426 2965 2997 6753 6785 2394
Size: 32 539 32 3756 32 2402
Fragments arranged by size:
3756 2402 539 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6793
XbaI T'CTAG_A
Cuts at: 4129 4129
Size: 6793
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3772 3772
Size: 6793
XhoI C'TCGA_G
Cuts at: 1943 3323 1943
Size: 1380 5413
XmaI C'CCGG_G
Cuts at: 1870 4118 1870
Size: 2248 4545
XmnI GAAnn'nnTTC
Cuts at: 4922 4922
Size: 6793
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4601 276
Size: 53 83 186 936 3067 2468
Fragments arranged by size:
3067 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI
BstZ17I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PspXI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI