Restriction map for
pFN21AA0737
(Circular) (Six-base) MAPSORT of: pFN21AA0737.seq Check: 3912 from: 1 to: 6568
pFN21AA0737 6568 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2506 2506
Size: 6568
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4378 278
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3911 1937
Size: 1974 4594
Acc65I G'GTAC_C
Cuts at: 3889 3889
Size: 6568
AclI AA'CG_TT
Cuts at: 1563 4695 5068 1563
Size: 3132 373 3063
Fragments arranged by size:
3132 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2532 2876 4631 6015 6283 1632
Size: 900 344 1755 1384 268 1917
Fragments arranged by size:
1917 1755 1384 900 344 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6371
AflIII A'CryG_T
Cuts at: 1203 5467 6444 1203
Size: 4264 977 1327
Fragments arranged by size:
4264 1327 977
AgeI A'CCGG_T
Cuts at: 5399 5399
Size: 6568
AhdI GACnn_n'nnGTC
Cuts at: 5297 5297
Size: 6568
AleI CACnn'nnGTG
Cuts at: 1166 3185 3854 1166
Size: 2019 669 3880
Fragments arranged by size:
3880 2019 669
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2507 1184
Size: 1323 5245
AlwNI CAG_nnn'CTG
Cuts at: 3290 3293 4311 5883 3290
Size: 3 1018 1572 3975
Fragments arranged by size:
3975 1572 1018 3
ApaLI G'TGCA_C
Cuts at: 4625 5781 4625
Size: 1156 5412
ApoI r'AATT_y
Cuts at: 1493 2111 3841 3877 4103 4255 6364 1493
Size: 618 1730 36 226 152 2109 1697
Fragments arranged by size:
2109 1730 1697 618 226 152 36
AseI AT'TA_AT
Cuts at: 160 1032 5122 160
Size: 872 4090 1606
Fragments arranged by size:
4090 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
AvaI C'yCGr_G
Cuts at: 1870 1943 3195 3893 1870
Size: 73 1252 698 4545
Fragments arranged by size:
4545 1252 698 73
AvrII C'CTAG_G
Cuts at: 2473 2473
Size: 6568
BamHI G'GATC_C
Cuts at: 2920 3898 4265 2920
Size: 978 367 5223
Fragments arranged by size:
5223 978 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2483 3595 3889 5344 618
Size: 325 217 627 696 1112 294 1455 1842
Fragments arranged by size:
1842 1455 1112 696 627 325 294 217
BanII G_rGCy'C
Cuts at: 1391 2301 3006 3204 3887 1391
Size: 910 705 198 683 4072
Fragments arranged by size:
4072 910 705 683 198
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2606 928
Size: 971 707 4890
Fragments arranged by size:
4890 971 707
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4759 4793 1903
Size: 34 2822 34 3678
Fragments arranged by size:
3678 2822 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4460 5670 4460
Size: 1210 5358
BclI T'GATC_A
Cuts at: 1548 2437 3624 1548
Size: 889 1187 4492
Fragments arranged by size:
4492 1187 889
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5179 136
Size: 107 122 71 631 605 3507 1525
Fragments arranged by size:
3507 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6563 6563
Size: 6568
BlpI GC'TnA_GC
Cuts at: 3985 3985
Size: 6568
Bme1580I G_kGCm'C
Cuts at: 1790 4629 5785 1790
Size: 2839 1156 2573
Fragments arranged by size:
2839 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6568
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2224 2497 3234
Size: 416 53 83 399 24 807 273 737
Cuts at: 3234 3772 4299 5257 442
Size: 538 527 958 1753
Fragments arranged by size:
1753 958 807 737 538 527 416 399
273 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6568
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3510 3705 3510
Size: 195 6373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2967 3096 3395 5228 1239
Size: 1728 129 299 1833 2579
Fragments arranged by size:
2579 1833 1728 299 129
Bpu10I CC'TnA_GC
Cuts at: 3552 3552
Size: 6568
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3135 4693 5558 5856 3135
Size: 1558 865 298 3847
Fragments arranged by size:
3847 1558 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3693 5231 882
Size: 617 2194 1538 2219
Fragments arranged by size:
2219 2194 1538 617
BsaAI yAC'GTr
Cuts at: 493 1204 4359 493
Size: 711 3155 2702
Fragments arranged by size:
3155 2702 711
BsaBI GATnn'nnATC
Cuts at: 2142 6562 2142
Size: 4420 2148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4375 4757 5392 275
Size: 53 83 186 936 2842 382 635 1451
Fragments arranged by size:
2842 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2577 5001 5399 5673 5820 6231 2577
Size: 2424 398 274 147 411 2914
Fragments arranged by size:
2914 2424 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2932 2962 3147 3177 1178
Size: 30 165 30 1529 30 185 30 4569
Fragments arranged by size:
4569 1529 185 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2816 3393 3459 3462 1186
Size: 1630 577 66 3 4292
Fragments arranged by size:
4292 1630 577 66 3
BseYI C'CCAG_C
Cuts at: 2457 2461 2591 2974 3080 3175 3708 5771 6267
Size: 4 130 383 106 95 533 2063 496
Cuts at: 6267 2457
Size: 2758
Fragments arranged by size:
2758 2063 533 496 383 130 106 95
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5995
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4018 4779 5807 1540
Size: 275 186 2017 761 1028 2301
Fragments arranged by size:
2301 2017 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4629 4714 5785 4629
Size: 85 1071 5412
Fragments arranged by size:
5412 1071 85
BsmI GAATG_Cn'
Cuts at: 4079 4172 4079
Size: 93 6475
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2301 3006 3204 3887 4629 4714 5785
Size: 399 511 705 198 683 742 85 1071
Cuts at: 5785 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 705 683 511 399 198
85
BspEI T'CCGG_A
Cuts at: 2577 6231 2577
Size: 3654 2914
BspHI T'CATG_A
Cuts at: 4457 6187 4457
Size: 1730 4838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2506 3923 844
Size: 1662 1417 3489
Fragments arranged by size:
3489 1662 1417
BsrBI CCG'CTC
Cuts at: 1394 1823 3556 4455 1394
Size: 429 1733 899 3507
Fragments arranged by size:
3507 1733 899 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3790 5063 5237 65
Size: 1183 2542 1273 174 1396
Fragments arranged by size:
2542 1396 1273 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5212 5399 1700
Size: 110 3402 187 2869
Fragments arranged by size:
3402 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6568
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6568
BssSI C'ACGA_G
Cuts at: 2555 4628 5640 2555
Size: 2073 1012 3483
Fragments arranged by size:
3483 2073 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2867 3290 3293 794
Size: 2073 423 3 4069
Fragments arranged by size:
4069 2073 423 3
BstBI TT'CG_AA
Cuts at: 3445 5372 3445
Size: 1927 4641
BstEII G'GTnAC_C
Cuts at: 1184 3187 1184
Size: 2003 4565
BstXI CCAn_nnnn'nTGG
Cuts at: 2326 2326
Size: 6568
BstYI r'GATC_y
Cuts at: 1972 2659 2920 3898 4265 4650 4667 6119 6205
Size: 687 261 978 367 385 17 1452 86
Cuts at: 6205 6217 6563 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 978 687 385 367 346 261
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3576 3642 3576
Size: 66 6502
BtgI C'CryG_G
Cuts at: 513 1065 2004 2272 2319 513
Size: 552 939 268 47 4762
Fragments arranged by size:
4762 939 552 268 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5829
Fragments arranged by size:
5829 683 56
BtsI GCAGTG_nn'
Cuts at: 3802 4084 4878 4898 3802
Size: 282 794 20 5472
Fragments arranged by size:
5472 794 282 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2754 2789 3834 3869 529
Size: 35 2190 35 1045 35 3228
Fragments arranged by size:
3228 2190 1045 35 35 35
DraI TTT'AAA
Cuts at: 3872 3872
Size: 6568
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5575 809
Size: 507 4259 1802
Fragments arranged by size:
4259 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2173 4015 4904
Size: 54 1425 50 275 18 343 1842 889
Cuts at: 4904 6533 8
Size: 1629 43
Fragments arranged by size:
1842 1629 1425 889 343 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4015 1537
Size: 275 2203 4090
Fragments arranged by size:
4090 2203 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2209 2639 3139 4498 1353
Size: 856 430 500 1359 3423
Fragments arranged by size:
3423 1359 856 500 430
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5155 5529 5675 387
Size: 1064 138 3566 374 146 1280
Fragments arranged by size:
3566 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2532 2876 2967 3096 3395 4631 5228
Size: 393 900 344 91 129 299 1236 597
Cuts at: 5228 6015 6283 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1236 900 787 597 393 344 299
268 129 91
EcoO109I rG'GnC_Cy
Cuts at: 2048 2635 2048
Size: 587 5981
EcoRI G'AATT_C
Cuts at: 3877 4255 3877
Size: 378 6190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2537 2537
Size: 6568
FspI TGC'GCA
Cuts at: 5074 5074
Size: 6568
HaeII r_GCGC'y
Cuts at: 1678 5715 1678
Size: 4037 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2384 2416 2963
Size: 32 135 32 614 32 166 32 547
Cuts at: 2963 2995 3247 3279 3635 3667 5209 5241 5283
Size: 32 252 32 356 32 1542 32 42
Cuts at: 5283 5315 1373
Size: 32 2626
Fragments arranged by size:
2626 1542 614 547 356 252 166 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2427 2971 3034 3245 3912 4158
Size: 988 281 489 544 63 211 667 246
Cuts at: 4158 669
Size: 3079
Fragments arranged by size:
3079 988 667 544 489 281 246 211
63
HindIII A'AGCT_T
Cuts at: 748 2095 748
Size: 1347 5221
HpaI GTT'AAC
Cuts at: 3034 4158 3034
Size: 1124 5444
KpnI G_GTAC'C
Cuts at: 3893 3893
Size: 6568
MfeI C'AATT_G
Cuts at: 2373 4167 2373
Size: 1794 4774
MluI A'CGCG_T
Cuts at: 6444 6444
Size: 6568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3229 5682 5866 1305
Size: 1924 2453 184 2007
Fragments arranged by size:
2453 2007 1924 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2175 10
Size: 54 1425 343 343 4403
Fragments arranged by size:
4403 1425 343 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2181 2258 2559 3185 3854 4526 4885
Size: 648 1015 77 301 626 669 672 359
Cuts at: 4885 5044 518
Size: 159 2042
Fragments arranged by size:
2042 1015 672 669 648 626 359 301
159 77
MspA1I CmG'CkG
Cuts at: 1606 2461 3290 3984 4661 5809 6054 1606
Size: 855 829 694 677 1148 245 2120
Fragments arranged by size:
2120 1148 855 829 694 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2272 2319 513
Size: 552 939 268 47 4762
Fragments arranged by size:
4762 939 552 268 47
NdeI CA'TA_TG
Cuts at: 387 2692 387
Size: 2305 4263
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6568
NotI GC'GGCC_GC
Cuts at: 4015 4015
Size: 6568
NspI r_CATG'y
Cuts at: 1132 1229 2257 3926 5471 1132
Size: 97 1028 1669 1545 2229
Fragments arranged by size:
2229 1669 1545 1028 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6568
PciI A'CATG_T
Cuts at: 5467 5467
Size: 6568
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2248 3589 1108
Size: 1140 1341 4087
Fragments arranged by size:
4087 1341 1140
PfoI T'CCnGG_A
Cuts at: 2014 2014
Size: 6568
PmeI GTTT'AAAC
Cuts at: 3872 3872
Size: 6568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4637 4669 6150 6182 4637
Size: 32 1481 32 5023
Fragments arranged by size:
5023 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6568
PsiI TTA'TAA
Cuts at: 4138 4138
Size: 6568
PstI C_TGCA'G
Cuts at: 830 3396 3480 3782 3809 3920 830
Size: 2566 84 302 27 111 3478
Fragments arranged by size:
3478 2566 302 111 84 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6568
PvuII CAG'CTG
Cuts at: 2461 3290 2461
Size: 829 5739
SalI G'TCGA_C
Cuts at: 1936 3910 1936
Size: 1974 4594
SbfI CC_TGCA'GG
Cuts at: 3920 3920
Size: 6568
ScaI AGT'ACT
Cuts at: 4816 6528 4816
Size: 1712 4856
SfcI C'TryA_G
Cuts at: 826 1045 2337 3332 3392 3476 3778 3805 3916
Size: 219 1292 995 60 84 302 27 111
Cuts at: 3916 5051 5732 5923 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1292 1135 995 681 302 219 191
111 84 60 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6568
SmaI CCC'GGG
Cuts at: 1872 3895 1872
Size: 2023 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3150 4672 5573 5835 820
Size: 197 926 1207 1522 901 262 1553
Fragments arranged by size:
1553 1522 1207 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4359 493
Size: 3866 2702
SpeI A'CTAG_T
Cuts at: 152 2543 152
Size: 2391 4177
SphI G_CATG'C
Cuts at: 1132 3926 1132
Size: 2794 3774
SspI AAT'ATT
Cuts at: 5 52 4492 5
Size: 47 4440 2081
Fragments arranged by size:
4440 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6568
StyI C'CwwG_G
Cuts at: 513 1065 2004 2133 2272 2319 2473 4007 513
Size: 552 939 129 139 47 154 1534 3074
Fragments arranged by size:
3074 1534 939 552 154 139 129 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2090 3255 4595 4612 4765 4950 5395
Size: 1255 510 1165 1340 17 153 185 445
Cuts at: 5395 325
Size: 1498
Fragments arranged by size:
1498 1340 1255 1165 510 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3221 4814 6526
Size: 274 80 33 51 1443 1244 1593 1712
Cuts at: 6526 96
Size: 138
Fragments arranged by size:
1712 1593 1443 1244 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6528 6560 6528
Size: 32 6536
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6568
XbaI T'CTAG_A
Cuts at: 3836 3904 3836
Size: 68 6500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3071 3071
Size: 6568
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6568
XmaI C'CCGG_G
Cuts at: 1870 3893 1870
Size: 2023 4545
XmnI GAAnn'nnTTC
Cuts at: 4697 4697
Size: 6568
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4376 276
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI