Restriction map for
pFN21AA0734
(Circular) (Six-base) MAPSORT of: pFN21AA0734.seq Check: 157 from: 1 to: 8266
pFN21AA0734 8266 bp
With 182 enzymes: SgfI *
March 28, 2012 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2140 2146 2506 3231 2140
Size: 6 360 725 7175
Fragments arranged by size:
7175 725 360 6
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3885 4821 6076 278
Size: 53 83 186 936 2349 936 1255 2468
Fragments arranged by size:
2468 2349 1255 936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2114 3087 5238 5609 1937
Size: 177 973 2151 371 4594
Fragments arranged by size:
4594 2151 973 371 177
Acc65I G'GTAC_C
Cuts at: 2459 5587 2459
Size: 3128 5138
AclI AA'CG_TT
Cuts at: 1563 6393 6766 1563
Size: 4830 373 3063
Fragments arranged by size:
4830 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2309 2632 2969 3638 3728 3812 4133 4958
Size: 677 323 337 669 90 84 321 825
Cuts at: 4958 6329 7713 7981 1632
Size: 1371 1384 268 1917
Fragments arranged by size:
1917 1384 1371 825 677 669 337 323
321 268 90 84
AfeI AGC'GCT
Cuts at: 2726 4025 2726
Size: 1299 6967
AflII C'TTAA_G
Cuts at: 820 1017 5137 820
Size: 197 4120 3949
Fragments arranged by size:
4120 3949 197
AflIII A'CryG_T
Cuts at: 1203 4320 7165 8142 1203
Size: 3117 2845 977 1327
Fragments arranged by size:
3117 2845 1327 977
AgeI A'CCGG_T
Cuts at: 5031 7097 5031
Size: 2066 6200
AhdI GACnn_n'nnGTC
Cuts at: 5329 6995 5329
Size: 1666 6600
AleI CACnn'nnGTG
Cuts at: 1166 2792 1166
Size: 1626 6640
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2241 1184
Size: 1057 7209
AlwNI CAG_nnn'CTG
Cuts at: 3285 3550 3784 4220 4297 4546 4801 5083 6009
Size: 265 234 436 77 249 255 282 926
Cuts at: 6009 7581 3285
Size: 1572 3970
Fragments arranged by size:
3970 1572 926 436 282 265 255 249
234 77
ApaI G_GGCC'C
Cuts at: 2039 2471 4503 5171 2039
Size: 432 2032 668 5134
Fragments arranged by size:
5134 2032 668 432
ApaLI G'TGCA_C
Cuts at: 2709 6323 7479 2709
Size: 3614 1156 3496
Fragments arranged by size:
3614 3496 1156
ApoI r'AATT_y
Cuts at: 1493 5575 5801 5953 8062 1493
Size: 4082 226 152 2109 1697
Fragments arranged by size:
4082 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6820 160
Size: 872 5788 1606
Fragments arranged by size:
5788 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8266
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2246 2324 3759 3982 4502 5591
Size: 73 70 233 78 1435 223 520 1089
Cuts at: 5591 1870
Size: 4545
Fragments arranged by size:
4545 1435 1089 520 233 223 78 73
70
AvrII C'CTAG_G
Cuts at: 5368 5368
Size: 8266
BamHI G'GATC_C
Cuts at: 2371 3738 5596 5963 2371
Size: 1367 1858 367 4674
Fragments arranged by size:
4674 1858 1367 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2459 2525 3045 3070
Size: 325 217 627 273 399 66 520 25
Cuts at: 3070 4301 5587 7042 618
Size: 1231 1286 1455 1842
Fragments arranged by size:
1842 1455 1286 1231 627 520 399 325
273 217 66 25
BanII G_rGCy'C
Cuts at: 1391 2039 2395 2471 2706 3273 3300 3340 3959
Size: 648 356 76 235 567 27 40 619
Cuts at: 3959 3990 3999 4182 4440 4503 5171 5461 5585
Size: 31 9 183 258 63 668 290 124
Cuts at: 5585 1391
Size: 4072
Fragments arranged by size:
4072 668 648 619 567 356 290 258
235 183 124 76 63 40 31 27
9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3877 5222 928
Size: 971 1978 1345 3972
Fragments arranged by size:
3972 1978 1345 971
BbvCI CC'TCA_GC
Cuts at: 3924 4124 4235 5477 3924
Size: 200 111 1242 6713
Fragments arranged by size:
6713 1242 200 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2050 2084 6457 6491 1903
Size: 34 113 34 4373 34 3678
Fragments arranged by size:
4373 3678 113 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2914 3172 6158 7368 2914
Size: 258 2986 1210 3812
Fragments arranged by size:
3812 2986 1210 258
BclI T'GATC_A
Cuts at: 1548 3162 1548
Size: 1614 6652
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2234 2952 5373
Size: 107 122 71 631 605 562 718 2421
Cuts at: 5373 6877 136
Size: 1504 1525
Fragments arranged by size:
2421 1525 1504 718 631 605 562 122
107 71
BglII A'GATC_T
Cuts at: 3660 5468 8261 3660
Size: 1808 2793 3665
Fragments arranged by size:
3665 2793 1808
BlpI GC'TnA_GC
Cuts at: 2501 3233 3299 3953 4529 5073 5683 2501
Size: 732 66 654 576 544 610 5084
Fragments arranged by size:
5084 732 654 610 576 544 66
Bme1580I G_kGCm'C
Cuts at: 1790 2039 2063 2165 2471 2713 4304 4503 5171
Size: 249 24 102 306 242 1591 199 668
Cuts at: 5171 6327 7483 1790
Size: 1156 1156 2573
Fragments arranged by size:
2573 1591 1156 1156 668 306 249 242
199 102 24
BmgBI CAC'GTC
Cuts at: 1318 4321 1318
Size: 3003 5263
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3472 5997 6955
Size: 416 53 83 399 24 2055 2525 958
Cuts at: 6955 442
Size: 1753
Fragments arranged by size:
2525 2055 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8266
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3130 3292 3314 3997 3130
Size: 162 22 683 7399
Fragments arranged by size:
7399 683 162 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2110 2495 3100 3125 3262 3464 3626 3929
Size: 871 385 605 25 137 202 162 303
Cuts at: 3929 3992 4004 4180 4196 4766 4778 4877 4925
Size: 63 12 176 16 570 12 99 48
Cuts at: 4925 5042 5561 6926 1239
Size: 117 519 1365 2579
Fragments arranged by size:
2579 1365 871 605 570 519 385 303
202 176 162 137 117 99 63 48
25 16 12 12
Bpu10I CC'TnA_GC
Cuts at: 2144 3924 4124 4235 4290 4745 5045 5477 2144
Size: 1780 200 111 55 455 300 432 4933
Fragments arranged by size:
4933 1780 455 432 300 200 111 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2555 3140 5516 6391 7256 7554 2555
Size: 585 2376 875 865 298 3267
Fragments arranged by size:
3267 2376 875 865 585 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2002 4232 6929 882
Size: 617 503 2230 2697 2219
Fragments arranged by size:
2697 2230 2219 617 503
BsaAI yAC'GTr
Cuts at: 493 1204 2586 6057 493
Size: 711 1382 3471 2702
Fragments arranged by size:
3471 2702 1382 711
BsaBI GATnn'nnATC
Cuts at: 8260 8260
Size: 8266
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2691 3084 3810 3882
Size: 53 83 186 936 1158 393 726 72
Cuts at: 3882 4188 4818 5041 5127 5376 6073 6455 7090
Size: 306 630 223 86 249 697 382 635
Cuts at: 7090 275
Size: 1451
Fragments arranged by size:
1451 1158 936 726 697 635 630 393
382 306 249 223 186 86 83 72
53
BsaWI w'CCGG_w
Cuts at: 5031 6699 7097 7371 7518 7929 5031
Size: 1668 398 274 147 411 5368
Fragments arranged by size:
5368 1668 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2420 2450 4178 4193 4208
Size: 30 165 30 1017 30 1728 15 15
Cuts at: 4208 4223 4751 4781 1178
Size: 15 528 30 4663
Fragments arranged by size:
4663 1728 1017 528 165 30 30 30
30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2107 2420 2441 2504 2552 3281 3306 3512
Size: 921 313 21 63 48 729 25 206
Cuts at: 3512 3941 3962 4183 4769 4958 5494 1186
Size: 429 21 221 586 189 536 3958
Fragments arranged by size:
3958 921 729 586 536 429 313 221
206 189 63 48 25 21 21
BseYI C'CCAG_C
Cuts at: 2375 2668 3433 4297 4305 4454 4801 5164 5348
Size: 293 765 864 8 149 347 363 184
Cuts at: 5348 5420 7469 7965 2375
Size: 72 2049 496 2676
Fragments arranged by size:
2676 2049 864 765 496 363 347 293
184 149 72 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2036 2174 2855 2941 3152 3314 3359
Size: 573 300 138 681 86 211 162 45
Cuts at: 3359 3394 4519 4940 5084 5213 5423 1163
Size: 35 1125 421 144 129 210 4006
Fragments arranged by size:
4006 1125 681 573 421 300 211 210
162 144 138 129 86 45 35
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 4389 5716 6477 7505 1540
Size: 275 186 1090 1298 1327 761 1028 2301
Fragments arranged by size:
2301 1327 1298 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 2544 2713 2832 2861 3258 3300 3999 4440
Size: 399 169 119 29 397 42 699 441
Cuts at: 4440 4515 6327 6412 7483 2145
Size: 75 1812 85 1071 2928
Fragments arranged by size:
2928 1812 1071 699 441 399 397 169
119 85 75 42 29
BsmI GAATG_Cn'
Cuts at: 5777 5870 5777
Size: 93 8173
BsmBI CGTCTCn'nnnn_
Cuts at: 4825 4825
Size: 8266
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2063 2145 2165 2395 2471 2544
Size: 399 249 24 82 20 230 76 73
Cuts at: 2544 2706 2713 2832 2861 3258 3273 3300 3340
Size: 162 7 119 29 397 15 27 40
Cuts at: 3340 3959 3990 3999 4182 4304 4440 4503 4515
Size: 619 31 9 183 122 136 63 12
Cuts at: 4515 5171 5461 5585 6327 6412 7483 1391
Size: 656 290 124 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 656 619 399 397 290
249 230 183 162 136 124 122 119
85 82 76 73 63 40 31 29
27 24 20 15 12 9 7
BspEI T'CCGG_A
Cuts at: 7929 7929
Size: 8266
BspHI T'CATG_A
Cuts at: 2890 4686 6155 7885 2890
Size: 1796 1469 1730 3271
Fragments arranged by size:
3271 1796 1730 1469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2058 2140 2146 2506 2952 3231 3373 3416
Size: 1214 82 6 360 446 279 142 43
Cuts at: 3416 3438 5121 5621 844
Size: 22 1683 500 3489
Fragments arranged by size:
3489 1683 1214 500 446 360 279 142
82 43 22 6
BsrBI CCG'CTC
Cuts at: 1394 1823 4059 4684 6153 1394
Size: 429 2236 625 1469 3507
Fragments arranged by size:
3507 2236 1469 625 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3693 6761 6935 65
Size: 1183 2445 3068 174 1396
Fragments arranged by size:
3068 2445 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4388 5031 5448 6910 7097 1700
Size: 110 2578 643 417 1462 187 2869
Fragments arranged by size:
2869 2578 1462 643 417 187 110
BsrGI T'GTAC_A
Cuts at: 96 2436 4627 96
Size: 2340 2191 3735
Fragments arranged by size:
3735 2340 2191
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8266
BssSI C'ACGA_G
Cuts at: 4439 6326 7338 4439
Size: 1887 1012 5367
Fragments arranged by size:
5367 1887 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2027 3550 4220 794
Size: 1233 1523 670 4840
Fragments arranged by size:
4840 1523 1233 670
BstBI TT'CG_AA
Cuts at: 7070 7070
Size: 8266
BstEII G'GTnAC_C
Cuts at: 1184 2045 2100 2785 1184
Size: 861 55 685 6665
Fragments arranged by size:
6665 861 685 55
BstYI r'GATC_y
Cuts at: 1972 2371 3660 3738 5468 5596 5963 6348 6365
Size: 399 1289 78 1730 128 367 385 17
Cuts at: 6365 7817 7903 7915 8261 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1730 1452 1289 399 385 367 346
128 86 78 17 12
BstZ17I GTA'TAC
Cuts at: 5239 5239
Size: 8266
Bsu36I CC'TnA_GG
Cuts at: 4913 4913
Size: 8266
BtgI C'CryG_G
Cuts at: 513 1065 2229 2463 4158 4182 4494 4674 5088
Size: 552 1164 234 1695 24 312 180 414
Cuts at: 5088 5106 513
Size: 18 3673
Fragments arranged by size:
3673 1695 1164 552 414 312 234 180
24 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7527
Fragments arranged by size:
7527 683 56
BtsI GCAGTG_nn'
Cuts at: 2347 3139 3479 4218 4404 5782 6576 6596 2347
Size: 792 340 739 186 1378 794 20 4017
Fragments arranged by size:
4017 1378 794 792 739 340 186 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2737 2772 529
Size: 35 2173 35 6023
Fragments arranged by size:
6023 2173 35 35
DraI TTT'AAA
Cuts at: 5570 5570
Size: 8266
DraIII CAC_nnn'GTG
Cuts at: 4579 4579
Size: 8266
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7273 809
Size: 507 5957 1802
Fragments arranged by size:
5957 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2628 3183 3366
Size: 54 1425 50 275 18 798 555 183
Cuts at: 3366 4045 4312 4386 4481 5085 5713 6602 8231
Size: 679 267 74 95 604 628 889 1629
Cuts at: 8231 8
Size: 43
Fragments arranged by size:
1629 1425 889 798 679 628 604 555
275 267 183 95 74 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4386 5713 1537
Size: 275 2574 1327 4090
Fragments arranged by size:
4090 2574 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2792 2843 3462 3647 3704 3863 4343 5255
Size: 1439 51 619 185 57 159 480 912
Cuts at: 5255 6196 1353
Size: 941 3423
Fragments arranged by size:
3423 1439 941 912 619 480 185 159
57 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2162 3511 5573 6853 7227 7373
Size: 1064 138 573 1349 2062 1280 374 146
Cuts at: 7373 387
Size: 1280
Fragments arranged by size:
2062 1349 1280 1280 1064 573 374 146
138
EcoICRI GAG'CTC
Cuts at: 3298 3997 4438 3298
Size: 699 441 7126
Fragments arranged by size:
7126 699 441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2309 2495 2632 2969 3100 3125
Size: 393 478 199 186 137 337 131 25
Cuts at: 3125 3262 3464 3626 3638 3728 3812 3929 3992
Size: 137 202 162 12 90 84 117 63
Cuts at: 3992 4004 4133 4180 4196 4766 4778 4877 4925
Size: 12 129 47 16 570 12 99 48
Cuts at: 4925 4958 5042 5561 6329 6926 7713 7981 1239
Size: 33 84 519 768 597 787 268 1524
Fragments arranged by size:
1524 787 768 597 570 519 478 393
337 268 202 199 186 162 137 137
131 129 117 99 90 84 84 63
48 47 33 25 16 12 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 4195 4195
Size: 8266
EcoO109I rG'GnC_Cy
Cuts at: 2191 2235 2329 2431 2468 2494 2578 2635 3011
Size: 44 94 102 37 26 84 57 376
Cuts at: 3011 4471 4499 4543 4762 4861 5016 5205 2191
Size: 1460 28 44 219 99 155 189 5252
Fragments arranged by size:
5252 1460 376 219 189 155 102 99
94 84 57 44 44 37 28 26
EcoRI G'AATT_C
Cuts at: 5575 5953 5575
Size: 378 7888
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2848 2848
Size: 8266
FspI TGC'GCA
Cuts at: 2339 4445 6772 2339
Size: 2106 2327 3833
Fragments arranged by size:
3833 2327 2106
HaeII r_GCGC'y
Cuts at: 1678 2211 2728 3784 4027 4412 4802 7413 1678
Size: 533 517 1056 243 385 390 2611 2531
Fragments arranged by size:
2611 2531 1056 533 517 390 385 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2888 2920 4043 4075 4178
Size: 32 135 32 1316 32 1123 32 103
Cuts at: 4178 4210 5315 5347 6907 6939 6981 7013 1373
Size: 32 1105 32 1560 32 42 32 2626
Fragments arranged by size:
2626 1560 1316 1123 1105 135 103 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2115 3088 5610 5856 669
Size: 988 281 177 973 2522 246 3079
Fragments arranged by size:
3079 2522 988 973 281 246 177
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8266
HpaI GTT'AAC
Cuts at: 5856 5856
Size: 8266
KpnI G_GTAC'C
Cuts at: 2463 5591 2463
Size: 3128 5138
MfeI C'AATT_G
Cuts at: 5865 5865
Size: 8266
MluI A'CGCG_T
Cuts at: 8142 8142
Size: 8266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3231 3657 3670 7380 7564 1305
Size: 1926 426 13 3710 184 2007
Fragments arranged by size:
3710 2007 1926 426 184 13
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2630 4314 4483 10
Size: 54 1425 343 798 1684 169 3793
Fragments arranged by size:
3793 1684 1425 798 343 169 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2792 3030 5312 6224 6583 6742 518
Size: 648 1626 238 2282 912 359 159 2042
Fragments arranged by size:
2282 2042 1626 912 648 359 238 159
MspA1I CmG'CkG
Cuts at: 1606 2033 2869 3093 3285 3364 3547 3593 3907
Size: 427 836 224 192 79 183 46 314
Cuts at: 3907 4533 5080 5108 5682 6359 7507 7752 1606
Size: 626 547 28 574 677 1148 245 2120
Fragments arranged by size:
2120 1148 836 677 626 574 547 427
314 245 224 192 183 79 46 28
NaeI GCC'GGC
Cuts at: 5450 5450
Size: 8266
NcoI C'CATG_G
Cuts at: 513 1065 2463 513
Size: 552 1398 6316
Fragments arranged by size:
6316 1398 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8266
NgoMIV G'CCGG_C
Cuts at: 5448 5448
Size: 8266
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8266
NotI GC'GGCC_GC
Cuts at: 5713 5713
Size: 8266
NspI r_CATG'y
Cuts at: 1132 1229 2278 2668 3454 5624 7169 1132
Size: 97 1049 390 786 2170 1545 2229
Fragments arranged by size:
2229 2170 1545 1049 786 390 97
PasI CC'CwG_GG
Cuts at: 1348 2195 1348
Size: 847 7419
PciI A'CATG_T
Cuts at: 7165 7165
Size: 8266
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3285 3604 1108
Size: 2177 319 5770
Fragments arranged by size:
5770 2177 319
PfoI T'CCnGG_A
Cuts at: 3063 4245 3063
Size: 1182 7084
PmeI GTTT'AAAC
Cuts at: 5570 5570
Size: 8266
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6335 6367 7848 7880 6335
Size: 32 1481 32 6721
Fragments arranged by size:
6721 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2329 2635 3011 5016 5205 2191
Size: 138 306 376 2005 189 5252
Fragments arranged by size:
5252 2005 376 306 189 138
PshAI GACnn'nnGTC
Cuts at: 1613 3753 1613
Size: 2140 6126
PsiI TTA'TAA
Cuts at: 5836 5836
Size: 8266
PspOMI G'GGCC_C
Cuts at: 2035 2467 4499 5167 2035
Size: 432 2032 668 5134
Fragments arranged by size:
5134 2032 668 432
PspXI vC'TCGA_Gb
Cuts at: 2324 2324
Size: 8266
PstI C_TGCA'G
Cuts at: 830 3546 3639 4797 5618 830
Size: 2716 93 1158 821 3478
Fragments arranged by size:
3478 2716 1158 821 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8266
PvuII CAG'CTG
Cuts at: 2869 3285 3364 3547 3907 4533 2869
Size: 416 79 183 360 626 6602
Fragments arranged by size:
6602 626 416 360 183 79
SacI G_AGCT'C
Cuts at: 3300 3999 4440 3300
Size: 699 441 7126
Fragments arranged by size:
7126 699 441
SacII CC_GC'GG
Cuts at: 5109 5109
Size: 8266
SalI G'TCGA_C
Cuts at: 1936 2113 3086 5608 1936
Size: 177 973 2522 4594
Fragments arranged by size:
4594 2522 973 177
SapI GCTCTTCn'nnn_
Cuts at: 2792 3647 2792
Size: 855 7411
SbfI CC_TGCA'GG
Cuts at: 5618 5618
Size: 8266
ScaI AGT'ACT
Cuts at: 6514 8226 6514
Size: 1712 6554
SexAI A'CCwGG_T
Cuts at: 2475 3147 2475
Size: 672 7594
SfcI C'TryA_G
Cuts at: 826 1045 3421 3542 3635 3838 4793 4834 5614
Size: 219 2376 121 93 203 955 41 780
Cuts at: 5614 6749 7430 7621 826
Size: 1135 681 191 1471
Fragments arranged by size:
2376 1471 1135 955 780 681 219 203
191 121 93 41
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8266
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8266
SmaI CCC'GGG
Cuts at: 1872 2015 2248 5593 1872
Size: 143 233 3345 4545
Fragments arranged by size:
4545 3345 233 143
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2324 2534 3155 5137 5531 6370
Size: 197 926 381 210 621 1982 394 839
Cuts at: 6370 7271 7533 820
Size: 901 262 1553
Fragments arranged by size:
1982 1553 926 901 839 621 394 381
262 210 197
SnaBI TAC'GTA
Cuts at: 493 6057 493
Size: 5564 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8266
SphI G_CATG'C
Cuts at: 1132 2668 5624 1132
Size: 1536 2956 3774
Fragments arranged by size:
3774 2956 1536
SspI AAT'ATT
Cuts at: 5 52 6190 5
Size: 47 6138 2081
Fragments arranged by size:
6138 2081 47
StuI AGG'CCT
Cuts at: 1517 4463 1517
Size: 2946 5320
StyI C'CwwG_G
Cuts at: 513 1065 2463 2574 2631 5368 5705 513
Size: 552 1398 111 57 2737 337 3074
Fragments arranged by size:
3074 2737 1398 552 337 111 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2088 3925 4246 4319 4793 6293 6310
Size: 1255 508 1837 321 73 474 1500 17
Cuts at: 6310 6463 6648 7093 325
Size: 153 185 445 1498
Fragments arranged by size:
1837 1500 1498 1255 508 474 445 321
185 153 73 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2436 4627 6512
Size: 274 80 33 51 1443 459 2191 1885
Cuts at: 6512 8224 96
Size: 1712 138
Fragments arranged by size:
2191 1885 1712 1443 459 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4193 4225 4751 4783 4949 4981 8226 8258 4193
Size: 32 526 32 166 32 3245 32 4201
Fragments arranged by size:
4201 3245 526 166 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3812 1316
Size: 2496 5770
XbaI T'CTAG_A
Cuts at: 5602 5602
Size: 8266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2519 2623 3967 2519
Size: 104 1344 6818
Fragments arranged by size:
6818 1344 104
XhoI C'TCGA_G
Cuts at: 1943 2324 1943
Size: 381 7885
XmaI C'CCGG_G
Cuts at: 1870 2013 2246 5591 1870
Size: 143 233 3345 4545
Fragments arranged by size:
4545 3345 233 143
XmnI GAAnn'nnTTC
Cuts at: 6395 6395
Size: 8266
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3883 4819 6074 276
Size: 53 83 186 936 2349 936 1255 2468
Fragments arranged by size:
2468 2349 1255 936 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BfrBI BsiWI BstXI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PsrI RsrII SanDI SfiI SfoI SrfI SwaI