Restriction map for
pFN21AA0732
(Circular) (Six-base) MAPSORT of: pFN21AA0732.seq Check: 6669 from: 1 to: 8962
pFN21AA0732 8962 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4973 4973
Size: 8962
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6772 278
Size: 53 83 186 936 5236 2468
Fragments arranged by size:
5236 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4337 4526 5180 6305 1937
Size: 2400 189 654 1125 4594
Fragments arranged by size:
4594 2400 1125 654 189
Acc65I G'GTAC_C
Cuts at: 4215 4266 6283 4215
Size: 51 2017 6894
Fragments arranged by size:
6894 2017 51
AclI AA'CG_TT
Cuts at: 1563 7089 7462 1563
Size: 5526 373 3063
Fragments arranged by size:
5526 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2900 4055 4292 5081 5417 5604 7025 8409
Size: 1268 1155 237 789 336 187 1421 1384
Cuts at: 8409 8677 1632
Size: 268 1917
Fragments arranged by size:
1917 1421 1384 1268 1155 789 336 268
237 187
AfeI AGC'GCT
Cuts at: 2732 2806 3878 2732
Size: 74 1072 7816
Fragments arranged by size:
7816 1072 74
AflII C'TTAA_G
Cuts at: 820 1017 4973 820
Size: 197 3956 4809
Fragments arranged by size:
4809 3956 197
AflIII A'CryG_T
Cuts at: 1203 7861 8838 1203
Size: 6658 977 1327
Fragments arranged by size:
6658 1327 977
AgeI A'CCGG_T
Cuts at: 7793 7793
Size: 8962
AhdI GACnn_n'nnGTC
Cuts at: 7691 7691
Size: 8962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5392 1184
Size: 4208 4754
AlwNI CAG_nnn'CTG
Cuts at: 3551 3745 3781 3931 4078 4751 5582 6705 8277
Size: 194 36 150 147 673 831 1123 1572
Cuts at: 8277 3551
Size: 4236
Fragments arranged by size:
4236 1572 1123 831 673 194 150 147
36
ApaI G_GGCC'C
Cuts at: 3605 6253 3605
Size: 2648 6314
ApaLI G'TGCA_C
Cuts at: 7019 8175 7019
Size: 1156 7806
ApoI r'AATT_y
Cuts at: 1493 2222 4484 6271 6497 6649 8758 1493
Size: 729 2262 1787 226 152 2109 1697
Fragments arranged by size:
2262 2109 1787 1697 729 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7516 160
Size: 872 6484 1606
Fragments arranged by size:
6484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8962
AvaI C'yCGr_G
Cuts at: 1870 1943 3701 5296 5945 6287 1870
Size: 73 1758 1595 649 342 4545
Fragments arranged by size:
4545 1758 1595 649 342 73
AvrII C'CTAG_G
Cuts at: 2369 2369
Size: 8962
BamHI G'GATC_C
Cuts at: 2055 3985 6292 6659 2055
Size: 1930 2307 367 4358
Fragments arranged by size:
4358 2307 1930 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3353 4215 4266 4302 5230
Size: 325 217 627 1566 862 51 36 928
Cuts at: 5230 5473 5827 6283 7738 618
Size: 243 354 456 1455 1842
Fragments arranged by size:
1842 1566 1455 928 862 627 456 354
325 243 217 51 36
BanII G_rGCy'C
Cuts at: 1391 2615 2723 3605 4035 4499 5727 5986 6253
Size: 1224 108 882 430 464 1228 259 267
Cuts at: 6253 6281 1391
Size: 28 4072
Fragments arranged by size:
4072 1228 1224 882 464 430 267 259
108 28
BbeI G_GCGC'C
Cuts at: 4306 4306
Size: 8962
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4113 4587 5034 5565 928
Size: 971 2214 474 447 531 4325
Fragments arranged by size:
4325 2214 971 531 474 447
BbvCI CC'TCA_GC
Cuts at: 2808 2808
Size: 8962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5648 5682 5917 5951 5989 6023 7153
Size: 34 3711 34 235 34 38 34 1130
Cuts at: 7153 7187 1903
Size: 34 3678
Fragments arranged by size:
3711 3678 1130 235 38 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3573 6854 8064 3573
Size: 3281 1210 4471
Fragments arranged by size:
4471 3281 1210
BclI T'GATC_A
Cuts at: 1548 3388 4170 5841 1548
Size: 1840 782 1671 4669
Fragments arranged by size:
4669 1840 1671 782
BfrBI ATG'CAT
Cuts at: 4188 4188
Size: 8962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 3351 5789
Size: 107 122 71 631 605 406 1273 2438
Cuts at: 5789 6145 7573 136
Size: 356 1428 1525
Fragments arranged by size:
2438 1525 1428 1273 631 605 406 356
122 107 71
BglII A'GATC_T
Cuts at: 2146 2436 4566 8957 2146
Size: 290 2130 4391 2151
Fragments arranged by size:
4391 2151 2130 290
BlpI GC'TnA_GC
Cuts at: 2785 3788 4992 5655 6379 2785
Size: 1003 1204 663 724 5368
Fragments arranged by size:
5368 1204 1003 724 663
Bme1580I G_kGCm'C
Cuts at: 1790 3605 4180 4607 5832 6253 7023 8179 1790
Size: 1815 575 427 1225 421 770 1156 2573
Fragments arranged by size:
2573 1815 1225 1156 770 575 427 421
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3553 3590 4653
Size: 416 53 83 399 24 2136 37 1063
Cuts at: 4653 5070 6693 7651 442
Size: 417 1623 958 1753
Fragments arranged by size:
2136 1753 1623 1063 958 417 416 399
83 53 37 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8962
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4912 5368 4912
Size: 456 8506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 3261 3787 3851 4583 5714 5822 7622
Size: 800 1222 526 64 732 1131 108 1800
Cuts at: 7622 1239
Size: 2579
Fragments arranged by size:
2579 1800 1222 1131 800 732 526 108
64
Bpu10I CC'TnA_GC
Cuts at: 2180 2808 3486 4493 6108 2180
Size: 628 678 1007 1615 5034
Fragments arranged by size:
5034 1615 1007 678 628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3169 4748 7087 7952 8250 3169
Size: 1579 2339 865 298 3881
Fragments arranged by size:
3881 2339 1579 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4433 7625 882
Size: 617 2934 3192 2219
Fragments arranged by size:
3192 2934 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5341 6246 6753 493
Size: 711 4137 905 507 2702
Fragments arranged by size:
4137 2702 905 711 507
BsaBI GATnn'nnATC
Cuts at: 8956 8956
Size: 8962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2140 3416 4303 6769
Size: 53 83 186 936 607 1276 887 2466
Cuts at: 6769 7151 7786 275
Size: 382 635 1451
Fragments arranged by size:
2466 1451 1276 936 887 635 607 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3820 6180 7395 7793 8067 8214 8625 3820
Size: 2360 1215 398 274 147 411 4157
Fragments arranged by size:
4157 2360 1215 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3785 3815 1178
Size: 30 165 30 2382 30 6325
Fragments arranged by size:
6325 2382 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 3719 4357 4901 5121 5552 5555 6149
Size: 1324 1209 638 544 220 431 3 594
Cuts at: 6149 1186
Size: 3999
Fragments arranged by size:
3999 1324 1209 638 594 544 431 220
3
BseYI C'CCAG_C
Cuts at: 3420 4179 4278 4642 4863 5891 6049 6232 8165
Size: 759 99 364 221 1028 158 183 1933
Cuts at: 8165 8661 3420
Size: 496 3721
Fragments arranged by size:
3721 1933 1028 759 496 364 221 183
158 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3731 4945 1163
Size: 573 1995 1214 5180
Fragments arranged by size:
5180 1995 1214 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6412 7173 8201 1540
Size: 275 186 4411 761 1028 2301
Fragments arranged by size:
4411 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4035 5727 7023 7108 8179 4035
Size: 1692 1296 85 1071 4818
Fragments arranged by size:
4818 1692 1296 1071 85
BsmI GAATG_Cn'
Cuts at: 3225 3946 5444 6473 6566 3225
Size: 721 1498 1029 93 5621
Fragments arranged by size:
5621 1498 1029 721 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2615 2723 3605 4035 4180 4499 4607
Size: 399 825 108 882 430 145 319 108
Cuts at: 4607 5727 5832 5986 6253 6281 7023 7108 8179
Size: 1120 105 154 267 28 742 85 1071
Cuts at: 8179 1391
Size: 2174
Fragments arranged by size:
2174 1120 1071 882 825 742 430 399
319 267 154 145 108 108 105 85
28
BspEI T'CCGG_A
Cuts at: 3820 8625 3820
Size: 4805 4157
BspHI T'CATG_A
Cuts at: 4422 6851 8581 4422
Size: 2429 1730 4803
Fragments arranged by size:
4803 2429 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2439 3260 3406 3738 3804 4072 4973 6195
Size: 1595 821 146 332 66 268 901 1222
Cuts at: 6195 6317 844
Size: 122 3489
Fragments arranged by size:
3489 1595 1222 901 821 332 268 146
122 66
BsrBI CCG'CTC
Cuts at: 1394 1823 2742 4896 6849 1394
Size: 429 919 2154 1953 3507
Fragments arranged by size:
3507 2154 1953 919 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4284 6007 7457 7631 65
Size: 1183 3036 1723 1450 174 1396
Fragments arranged by size:
3036 1723 1450 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7606 7793 1700
Size: 110 5796 187 2869
Fragments arranged by size:
5796 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5238 96
Size: 5142 3820
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8962
BssSI C'ACGA_G
Cuts at: 7022 8034 7022
Size: 1012 7950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4194 794
Size: 3400 5562
BstBI TT'CG_AA
Cuts at: 7766 7766
Size: 8962
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8962
BstXI CCAn_nnnn'nTGG
Cuts at: 2491 4561 4703 5143 5632 5891 2491
Size: 2070 142 440 489 259 5562
Fragments arranged by size:
5562 2070 489 440 259 142
BstYI r'GATC_y
Cuts at: 1972 2031 2055 2146 2436 3314 3329 3927 3985
Size: 59 24 91 290 878 15 598 58
Cuts at: 3985 4566 5146 5679 6292 6659 7044 7061 8513
Size: 581 580 533 613 367 385 17 1452
Cuts at: 8513 8599 8611 8957 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 878 613 598 581 580 533
385 367 346 290 91 86 59 58
24 17 15 12
BstZ17I GTA'TAC
Cuts at: 4338 4338
Size: 8962
Bsu36I CC'TnA_GG
Cuts at: 5007 5325 5007
Size: 318 8644
BtgI C'CryG_G
Cuts at: 513 1065 2004 2707 3004 3096 3346 4589 4702
Size: 552 939 703 297 92 250 1243 113
Cuts at: 4702 5490 5995 513
Size: 788 505 3480
Fragments arranged by size:
3480 1243 939 788 703 552 505 297
250 113 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4198 6001 487
Size: 683 56 2972 1803 3448
Fragments arranged by size:
3448 2972 1803 683 56
BtsI GCAGTG_nn'
Cuts at: 3160 4338 4752 5220 6034 6478 7272 7292 3160
Size: 1178 414 468 814 444 794 20 4830
Fragments arranged by size:
4830 1178 814 794 468 444 414 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8927
DraI TTT'AAA
Cuts at: 6266 6266
Size: 8962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7969 809
Size: 507 6653 1802
Fragments arranged by size:
6653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3001 4932 5640
Size: 54 1425 50 275 18 1171 1931 708
Cuts at: 5640 5781 5862 6043 6409 7298 8927 8
Size: 141 81 181 366 889 1629 43
Fragments arranged by size:
1931 1629 1425 1171 889 708 366 275
181 141 81 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6409 1537
Size: 275 4597 4090
Fragments arranged by size:
4597 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2969 3057 4431 5274 5424 6892 1353
Size: 1616 88 1374 843 150 1468 3423
Fragments arranged by size:
3423 1616 1468 1374 843 150 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2470 4525 4943 4972 7549
Size: 1064 138 435 446 2055 418 29 2577
Cuts at: 7549 7923 8069 387
Size: 374 146 1280
Fragments arranged by size:
2577 2055 1280 1064 446 435 418 374
146 138 29
EcoICRI GAG'CTC
Cuts at: 4033 5725 4033
Size: 1692 7270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2900 3261 3787 3851 4055 4292
Size: 393 407 861 361 526 64 204 237
Cuts at: 4292 4583 5081 5417 5604 5714 5822 7025 7622
Size: 291 498 336 187 110 108 1203 597
Cuts at: 7622 8409 8677 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1203 861 787 597 526 498 407
393 361 336 291 268 237 204 187
110 108 64
EcoNI CCTnn'n_nnAGG
Cuts at: 2368 2513 3141 5279 5864 2368
Size: 145 628 2138 585 5466
Fragments arranged by size:
5466 2138 628 585 145
EcoO109I rG'GnC_Cy
Cuts at: 2951 3296 3373 3548 3602 3626 3826 4759 5245
Size: 345 77 175 54 24 200 933 486
Cuts at: 5245 5329 2951
Size: 84 6584
Fragments arranged by size:
6584 933 486 345 200 175 84 77
54 24
EcoRI G'AATT_C
Cuts at: 2222 6271 6649 2222
Size: 4049 378 4535
Fragments arranged by size:
4535 4049 378
FspI TGC'GCA
Cuts at: 4376 7468 4376
Size: 3092 5870
HaeII r_GCGC'y
Cuts at: 1678 2734 2808 3880 4306 6203 8109 1678
Size: 1056 74 1072 426 1897 1906 2531
Fragments arranged by size:
2531 1906 1897 1072 1056 426 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4865 4897 5138 5162 5170
Size: 32 135 32 3293 32 241 24 8
Cuts at: 5170 5194 7603 7635 7677 7709 1373
Size: 24 2409 32 42 32 2626
Fragments arranged by size:
3293 2626 2409 241 135 42 32 32
32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 5181 6171 6306 6552 669
Size: 988 281 3243 990 135 246 3079
Fragments arranged by size:
3243 3079 990 988 281 246 135
HindIII A'AGCT_T
Cuts at: 748 4349 748
Size: 3601 5361
HpaI GTT'AAC
Cuts at: 6552 6552
Size: 8962
KasI G'GCGC_C
Cuts at: 4302 4302
Size: 8962
KpnI G_GTAC'C
Cuts at: 4219 4270 6287 4219
Size: 51 2017 6894
Fragments arranged by size:
6894 2017 51
MfeI C'AATT_G
Cuts at: 6561 6561
Size: 8962
MluI A'CGCG_T
Cuts at: 8838 8838
Size: 8962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2022 4522 8076 8260 1305
Size: 717 2500 3554 184 2007
Fragments arranged by size:
3554 2500 2007 717 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4934 5642 5783 5864 6045
Size: 54 1425 343 3102 708 141 81 181
Cuts at: 6045 10
Size: 2927
Fragments arranged by size:
3102 2927 1425 708 343 181 141 81
54
MslI CAynn'nnrTG
Cuts at: 518 1166 4701 4841 5036 5822 6920 7279 7438
Size: 648 3535 140 195 786 1098 359 159
Cuts at: 7438 518
Size: 2042
Fragments arranged by size:
3535 2042 1098 786 648 359 195 159
140
MspA1I CmG'CkG
Cuts at: 1606 2709 2789 4867 4959 4996 5372 5592 6049
Size: 1103 80 2078 92 37 376 220 457
Cuts at: 6049 6378 7055 8203 8448 1606
Size: 329 677 1148 245 2120
Fragments arranged by size:
2120 2078 1148 1103 677 457 376 329
245 220 92 80 37
NarI GG'CG_CC
Cuts at: 4303 4303
Size: 8962
NcoI C'CATG_G
Cuts at: 513 1065 2004 3096 4702 5490 513
Size: 552 939 1092 1606 788 3985
Fragments arranged by size:
3985 1606 1092 939 788 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8962
NotI GC'GGCC_GC
Cuts at: 6409 6409
Size: 8962
NsiI A_TGCA'T
Cuts at: 4190 4190
Size: 8962
NspI r_CATG'y
Cuts at: 1132 1229 4541 4822 5162 6320 7865 1132
Size: 97 3312 281 340 1158 1545 2229
Fragments arranged by size:
3312 2229 1545 1158 340 281 97
PasI CC'CwG_GG
Cuts at: 1348 2210 3623 4600 1348
Size: 862 1413 977 5710
Fragments arranged by size:
5710 1413 977 862
PciI A'CATG_T
Cuts at: 7861 7861
Size: 8962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2621 1108
Size: 1513 7449
PfoI T'CCnGG_A
Cuts at: 4905 5052 5274 5691 4905
Size: 147 222 417 8176
Fragments arranged by size:
8176 417 222 147
PmeI GTTT'AAAC
Cuts at: 6266 6266
Size: 8962
PmlI CAC'GTG
Cuts at: 6246 6246
Size: 8962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3771 3803 7031 7063 8544 8576 3771
Size: 32 3228 32 1481 32 4157
Fragments arranged by size:
4157 3228 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2951 3296 3373 4759 5245 5329 2951
Size: 345 77 1386 486 84 6584
Fragments arranged by size:
6584 1386 486 345 84 77
PshAI GACnn'nnGTC
Cuts at: 1613 5703 1613
Size: 4090 4872
PsiI TTA'TAA
Cuts at: 6532 6532
Size: 8962
PspOMI G'GGCC_C
Cuts at: 3601 6249 3601
Size: 2648 6314
PspXI vC'TCGA_Gb
Cuts at: 5945 5945
Size: 8962
PstI C_TGCA'G
Cuts at: 830 2436 3735 3786 3867 4083 4747 4788 4827
Size: 1606 1299 51 81 216 664 41 39
Cuts at: 4827 5215 6314 830
Size: 388 1099 3478
Fragments arranged by size:
3478 1606 1299 1099 664 388 216 81
51 41 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8962
PvuII CAG'CTG
Cuts at: 2789 4867 4996 5372 6049 2789
Size: 2078 129 376 677 5702
Fragments arranged by size:
5702 2078 677 376 129
SacI G_AGCT'C
Cuts at: 4035 5727 4035
Size: 1692 7270
SacII CC_GC'GG
Cuts at: 2710 2710
Size: 8962
SalI G'TCGA_C
Cuts at: 1936 5179 6304 1936
Size: 3243 1125 4594
Fragments arranged by size:
4594 3243 1125
SanDI GG'GwC_CC
Cuts at: 3373 3373
Size: 8962
SapI GCTCTTCn'nnn_
Cuts at: 5424 5424
Size: 8962
SbfI CC_TGCA'GG
Cuts at: 6314 6314
Size: 8962
ScaI AGT'ACT
Cuts at: 7210 8922 7210
Size: 1712 7250
SexAI A'CCwGG_T
Cuts at: 2514 2514
Size: 8962
SfcI C'TryA_G
Cuts at: 826 1045 2331 2432 3587 3731 3782 3863 4079
Size: 219 1286 101 1155 144 51 81 216
Cuts at: 4079 4743 4784 4823 5211 6310 7445 8126 8317
Size: 664 41 39 388 1099 1135 681 191
Cuts at: 8317 826
Size: 1471
Fragments arranged by size:
1471 1286 1155 1135 1099 681 664 388
219 216 191 144 101 81 51 41
39
SfiI GGCCn_nnn'nGGCC
Cuts at: 2078 2078
Size: 8962
SfoI GGC'GCC
Cuts at: 4304 4304
Size: 8962
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8962
SmaI CCC'GGG
Cuts at: 1872 6289 1872
Size: 4417 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3148 4763 4973 5945 7066 7967
Size: 197 926 1205 1615 210 972 1121 901
Cuts at: 7967 8229 820
Size: 262 1553
Fragments arranged by size:
1615 1553 1205 1121 972 926 901 262
210 197
SnaBI TAC'GTA
Cuts at: 493 6753 493
Size: 6260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8962
SphI G_CATG'C
Cuts at: 1132 4822 6320 1132
Size: 3690 1498 3774
Fragments arranged by size:
3774 3690 1498
SspI AAT'ATT
Cuts at: 5 52 6886 5
Size: 47 6834 2081
Fragments arranged by size:
6834 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8962
StyI C'CwwG_G
Cuts at: 513 1065 2004 2369 2862 3096 4198 4410 4702
Size: 552 939 365 493 234 1102 212 292
Cuts at: 4702 5490 5865 6401 513
Size: 788 375 536 3074
Fragments arranged by size:
3074 1102 939 788 552 536 493 375
365 292 234 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2262 6989 7006 7159 7344 7789 325
Size: 1255 682 4727 17 153 185 445 1498
Fragments arranged by size:
4727 1498 1255 682 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4852 5238 5814
Size: 274 80 33 51 1443 2875 386 576
Cuts at: 5814 7208 8920 96
Size: 1394 1712 138
Fragments arranged by size:
2875 1712 1443 1394 576 386 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2531 2563 3651 3682 3683 3714 5110 5142 5448
Size: 32 1088 31 1 31 1396 32 306
Cuts at: 5448 5480 6175 6207 8922 8954 2531
Size: 32 695 32 2715 32 2539
Fragments arranged by size:
2715 2539 1396 1088 695 306 32 32
32 32 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 1316 3652 4358 1316
Size: 2336 706 5920
Fragments arranged by size:
5920 2336 706
XbaI T'CTAG_A
Cuts at: 6298 6298
Size: 8962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2321 3118 2321
Size: 797 8165
XhoI C'TCGA_G
Cuts at: 1943 5945 1943
Size: 4002 4960
XmaI C'CCGG_G
Cuts at: 1870 6287 1870
Size: 4417 4545
XmnI GAAnn'nnTTC
Cuts at: 2364 3944 4011 7091 2364
Size: 1580 67 3080 4235
Fragments arranged by size:
4235 3080 1580 67
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6770 276
Size: 53 83 186 936 5236 2468
Fragments arranged by size:
5236 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BsiWI BsmBI DraIII EcoRV FalI
FseI FspAI NaeI NgoMIV NruI PacI PsrI RsrII
SrfI SwaI