Restriction map for
pFN21AA0721
(Circular) (Six-base) MAPSORT of: pFN21AA0721.seq Check: 830 from: 1 to: 5947
pFN21AA0721 5947 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2205 3290 1937
Size: 268 1085 4594
Fragments arranged by size:
4594 1085 268
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2666 3214 4010 5394 5662 1632
Size: 1034 548 796 1384 268 1917
Fragments arranged by size:
1917 1384 1034 796 548 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 4846 5823 1203
Size: 3643 977 1327
Fragments arranged by size:
3643 1327 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2295 1184
Size: 1111 4836
AlwNI CAG_nnn'CTG
Cuts at: 2315 3690 5262 2315
Size: 1375 1572 3000
Fragments arranged by size:
3000 1572 1375
ApaI G_GGCC'C
Cuts at: 2596 3177 2596
Size: 581 5366
ApaLI G'TGCA_C
Cuts at: 4004 5160 4004
Size: 1156 4791
ApoI r'AATT_y
Cuts at: 1493 2888 3098 3186 3256 3482 3634 5743 1493
Size: 1395 210 88 70 226 152 2109 1697
Fragments arranged by size:
2109 1697 1395 226 210 152 88 70
AseI AT'TA_AT
Cuts at: 160 1032 4501 160
Size: 872 3469 1606
Fragments arranged by size:
3469 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 3272 1870
Size: 73 1329 4545
Fragments arranged by size:
4545 1329 73
BamHI G'GATC_C
Cuts at: 2197 3277 3644 2197
Size: 1080 367 4500
Fragments arranged by size:
4500 1080 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3000 3268 4723 618
Size: 325 217 627 1213 268 1455 1842
Fragments arranged by size:
1842 1455 1213 627 325 268 217
BanII G_rGCy'C
Cuts at: 1391 2112 2370 2596 3177 3266 1391
Size: 721 258 226 581 89 4072
Fragments arranged by size:
4072 721 581 258 226 89
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2829 928
Size: 971 930 4046
Fragments arranged by size:
4046 971 930
BbvCI CC'TCA_GC
Cuts at: 2831 2831
Size: 5947
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2082 2116 3092 3126 4138 4172 1903
Size: 34 145 34 976 34 1012 34 3678
Fragments arranged by size:
3678 1012 976 145 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3839 5049 3839
Size: 1210 4737
BclI T'GATC_A
Cuts at: 1548 2805 2841 1548
Size: 1257 36 4654
Fragments arranged by size:
4654 1257 36
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2324 2483 4558
Size: 107 122 71 631 605 652 159 2075
Cuts at: 4558 136
Size: 1525
Fragments arranged by size:
2075 1525 652 631 605 159 122 107
71
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 3364 3364
Size: 5947
Bme1580I G_kGCm'C
Cuts at: 1790 2596 3177 4008 5164 1790
Size: 806 581 831 1156 2573
Fragments arranged by size:
2573 1156 831 806 581
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2225 3678 4636
Size: 416 53 83 399 24 808 1453 958
Cuts at: 4636 442
Size: 1753
Fragments arranged by size:
1753 1453 958 808 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 2645 4607 1239
Size: 947 459 1962 2579
Fragments arranged by size:
2579 1962 947 459
Bpu10I CC'TnA_GC
Cuts at: 2831 2831
Size: 5947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2708 4072 4937 5235 2708
Size: 1364 865 298 3420
Fragments arranged by size:
3420 1364 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2088 2162 2430 4610 882
Size: 617 589 74 268 2180 2219
Fragments arranged by size:
2219 2180 617 589 268 74
BsaAI yAC'GTr
Cuts at: 493 1204 3738 493
Size: 711 2534 2702
Fragments arranged by size:
2702 2534 711
BsaBI GATnn'nnATC
Cuts at: 5941 5941
Size: 5947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3754 4136 4771 275
Size: 53 83 186 936 2221 382 635 1451
Fragments arranged by size:
2221 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2086 4380 4778 5052 5199 5610 2086
Size: 2294 398 274 147 411 2423
Fragments arranged by size:
2423 2294 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5722
Fragments arranged by size:
5722 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2591 2873 1186
Size: 1405 282 4260
Fragments arranged by size:
4260 1405 282
BseYI C'CCAG_C
Cuts at: 2381 2790 5150 5646 2381
Size: 409 2360 496 2682
Fragments arranged by size:
2682 2360 496 409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2274 2561 1163
Size: 573 538 287 4549
Fragments arranged by size:
4549 573 538 287
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3397 4158 5186 1540
Size: 275 186 1396 761 1028 2301
Fragments arranged by size:
2301 1396 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4008 4093 5164 4008
Size: 85 1071 4791
Fragments arranged by size:
4791 1071 85
BsmI GAATG_Cn'
Cuts at: 3458 3551 3458
Size: 93 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2370 2596 3177 3266 4008 4093
Size: 399 322 258 226 581 89 742 85
Cuts at: 4093 5164 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 581 399 322 258 226
89 85
BspEI T'CCGG_A
Cuts at: 2086 5610 2086
Size: 3524 2423
BspHI T'CATG_A
Cuts at: 3836 5566 3836
Size: 1730 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3302 844
Size: 2458 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 3834 1394
Size: 429 187 1824 3507
Fragments arranged by size:
3507 1824 429 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 4442 4616 65
Size: 1183 3194 174 1396
Fragments arranged by size:
3194 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 4591 4778 1700
Size: 110 238 2543 187 2869
Fragments arranged by size:
2869 2543 238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5947
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 4007 5019 4007
Size: 1012 4935
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2315 794
Size: 1521 4426
BstBI TT'CG_AA
Cuts at: 2779 4751 2779
Size: 1972 3975
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstXI CCAn_nnnn'nTGG
Cuts at: 2348 2763 2997 2348
Size: 415 234 5298
Fragments arranged by size:
5298 415 234
BstYI r'GATC_y
Cuts at: 1972 2083 2197 2957 3224 3277 3644 4029 4046
Size: 111 114 760 267 53 367 385 17
Cuts at: 4046 5498 5584 5596 5942 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 760 385 367 346 267 114
111 86 53 17 12
Bsu36I CC'TnA_GG
Cuts at: 2077 2077
Size: 5947
BtgI C'CryG_G
Cuts at: 513 1065 2371 513
Size: 552 1306 4089
Fragments arranged by size:
4089 1306 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2534 487
Size: 683 56 1308 3900
Fragments arranged by size:
3900 1308 683 56
BtsI GCAGTG_nn'
Cuts at: 2496 3463 4257 4277 2496
Size: 967 794 20 4166
Fragments arranged by size:
4166 967 794 20
ClaI AT'CG_AT
Cuts at: 1554 2634 1554
Size: 1080 4867
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 3251 3251
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2701 2812 2965
Size: 54 1425 50 275 18 871 111 153
Cuts at: 2965 3394 4283 5912 8
Size: 429 889 1629 43
Fragments arranged by size:
1629 1425 889 871 429 275 153 111
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3394 1537
Size: 275 1582 4090
Fragments arranged by size:
4090 1582 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3877 1353
Size: 2524 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4534 4908 5054 387
Size: 1064 138 2945 374 146 1280
Fragments arranged by size:
2945 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2645 2666 3214 4010 4607 5394
Size: 393 554 459 21 548 796 597 787
Cuts at: 5394 5662 1239
Size: 268 1524
Fragments arranged by size:
1524 796 787 597 554 548 459 393
268 21
EcoO109I rG'GnC_Cy
Cuts at: 2592 2593 2673 3173 3174 2592
Size: 1 80 500 1 5365
Fragments arranged by size:
5365 500 80 1 1
EcoRI G'AATT_C
Cuts at: 3098 3186 3256 3634 3098
Size: 88 70 378 5411
Fragments arranged by size:
5411 378 88 70
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 5947
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 3222 5094 1678
Size: 1544 1872 2531
Fragments arranged by size:
2531 1872 1544
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 4588 4620 4662
Size: 32 135 32 488 32 2496 32 42
Cuts at: 4662 4694 1373
Size: 32 2626
Fragments arranged by size:
2626 2496 488 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 3291 3537 669
Size: 988 281 268 1085 246 3079
Fragments arranged by size:
3079 1085 988 281 268 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5947
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 3546 3546
Size: 5947
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5061 5245 1305
Size: 3756 184 2007
Fragments arranged by size:
3756 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2814 10
Size: 54 1425 343 982 3143
Fragments arranged by size:
3143 1425 982 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3905 4264 4423 518
Size: 648 2739 359 159 2042
Fragments arranged by size:
2739 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2794 2997 3363 4040 5188 5433 1606
Size: 1188 203 366 677 1148 245 2120
Fragments arranged by size:
2120 1188 1148 677 366 245 203
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5395
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5947
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 2710 2725 2830 3305 4850 1132
Size: 97 1481 15 105 475 1545 2229
Fragments arranged by size:
2229 1545 1481 475 105 97 15
PasI CC'CwG_GG
Cuts at: 1348 2589 1348
Size: 1241 4706
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5947
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5947
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PspOMI G'GGCC_C
Cuts at: 2592 3173 2592
Size: 581 5366
PstI C_TGCA'G
Cuts at: 830 3299 830
Size: 2469 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
PvuII CAG'CTG
Cuts at: 2794 2794
Size: 5947
RsrII CG'GwC_CG
Cuts at: 2089 2089
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 2204 3289 1936
Size: 268 1085 4594
Fragments arranged by size:
4594 1085 268
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SfcI C'TryA_G
Cuts at: 826 1045 3295 4430 5111 5302 826
Size: 219 2250 1135 681 191 1471
Fragments arranged by size:
2250 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 3274 1872
Size: 1402 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2687 4051 4952 5214 820
Size: 197 926 744 1364 901 262 1553
Fragments arranged by size:
1553 1364 926 901 744 262 197
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 2710 3305 1132
Size: 1578 595 3774
Fragments arranged by size:
3774 1578 595
SspI AAT'ATT
Cuts at: 5 52 3871 5
Size: 47 3819 2081
Fragments arranged by size:
3819 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5947
StyI C'CwwG_G
Cuts at: 513 1065 2104 2451 3200 3386 513
Size: 552 1039 347 749 186 3074
Fragments arranged by size:
3074 1039 749 552 347 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2106 3155 3974 3991 4144 4329 4774
Size: 1255 526 1049 819 17 153 185 445
Cuts at: 4774 325
Size: 1498
Fragments arranged by size:
1498 1255 1049 819 526 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4193 5905 96
Size: 274 80 33 51 1443 2216 1712 138
Fragments arranged by size:
2216 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5907 5939 5907
Size: 32 5915
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 2395 3094 3283 2395
Size: 699 189 5059
Fragments arranged by size:
5059 699 189
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5947
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 3272 1870
Size: 1402 4545
XmnI GAAnn'nnTTC
Cuts at: 4076 4076
Size: 5947
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI