Restriction map for
pFN21AA0691
(Circular) (Six-base) MAPSORT of: pFN21AA0691.seq Check: 5667 from: 1 to: 6964
pFN21AA0691 6964 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2407 2407
Size: 6964
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4774 278
Size: 53 83 186 936 3238 2468
Fragments arranged by size:
3238 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4004 4307 1937
Size: 2067 303 4594
Fragments arranged by size:
4594 2067 303
Acc65I G'GTAC_C
Cuts at: 4285 4285
Size: 6964
AclI AA'CG_TT
Cuts at: 1563 5091 5464 1563
Size: 3528 373 3063
Fragments arranged by size:
3528 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2871 3523 5027 6411 6679 1632
Size: 1239 652 1504 1384 268 1917
Fragments arranged by size:
1917 1504 1384 1239 652 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6767
AflIII A'CryG_T
Cuts at: 1203 5863 6840 1203
Size: 4660 977 1327
Fragments arranged by size:
4660 1327 977
AgeI A'CCGG_T
Cuts at: 5795 5795
Size: 6964
AhdI GACnn_n'nnGTC
Cuts at: 5693 5693
Size: 6964
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6964
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6964
AlwNI CAG_nnn'CTG
Cuts at: 2716 4000 4060 4707 6279 2716
Size: 1284 60 647 1572 3401
Fragments arranged by size:
3401 1572 1284 647 60
ApaI G_GGCC'C
Cuts at: 3068 3153 3374 3815 3068
Size: 85 221 441 6217
Fragments arranged by size:
6217 441 221 85
ApaLI G'TGCA_C
Cuts at: 5021 6177 5021
Size: 1156 5808
ApoI r'AATT_y
Cuts at: 1493 3986 4273 4499 4651 6760 1493
Size: 2493 287 226 152 2109 1697
Fragments arranged by size:
2493 2109 1697 287 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5518 160
Size: 872 4486 1606
Fragments arranged by size:
4486 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
AvaI C'yCGr_G
Cuts at: 1870 1943 2696 3097 4289 1870
Size: 73 753 401 1192 4545
Fragments arranged by size:
4545 1192 753 401 73
BamHI G'GATC_C
Cuts at: 4294 4661 4294
Size: 367 6597
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3101 3278 3496 3560 3870
Size: 325 217 627 1314 177 218 64 310
Cuts at: 3870 4169 4285 5740 618
Size: 299 116 1455 1842
Fragments arranged by size:
1842 1455 1314 627 325 310 299 218
217 177 116 64
BanII G_rGCy'C
Cuts at: 1391 3068 3153 3374 3629 3815 4140 4283 1391
Size: 1677 85 221 255 186 325 143 4072
Fragments arranged by size:
4072 1677 325 255 221 186 143 85
BbeI G_GCGC'C
Cuts at: 3282 3282
Size: 6964
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5993
BbvCI CC'TCA_GC
Cuts at: 2177 3745 2177
Size: 1568 5396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5155 5189 1903
Size: 34 3218 34 3678
Fragments arranged by size:
3678 3218 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4856 6066 4856
Size: 1210 5754
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6964
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5575 136
Size: 107 122 71 631 605 3903 1525
Fragments arranged by size:
3903 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2760 6959 2760
Size: 4199 2765
BlpI GC'TnA_GC
Cuts at: 4381 4381
Size: 6964
Bme1580I G_kGCm'C
Cuts at: 1790 3061 3068 3104 3153 3374 3565 3815 3822
Size: 1271 7 36 49 221 191 250 7
Cuts at: 3822 4046 4172 5025 6181 1790
Size: 224 126 853 1156 2573
Fragments arranged by size:
2573 1271 1156 853 250 224 221 191
126 49 36 7 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6964
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 3414 3818
Size: 416 53 83 399 24 1721 276 404
Cuts at: 3818 4695 5653 442
Size: 877 958 1753
Fragments arranged by size:
1753 1721 958 877 416 404 399 276
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6964
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3030 3030
Size: 6964
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2785 2886 4058 4244 4265 5624 1239
Size: 1546 101 1172 186 21 1359 2579
Fragments arranged by size:
2579 1546 1359 1172 186 101 21
Bpu10I CC'TnA_GC
Cuts at: 2177 3463 3615 3719 3745 2177
Size: 1286 152 104 26 5396
Fragments arranged by size:
5396 1286 152 104 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2033 3025 5089 5954 6252 2033
Size: 992 2064 865 298 2745
Fragments arranged by size:
2745 2064 992 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2288 2549 5627 882
Size: 617 789 261 3078 2219
Fragments arranged by size:
3078 2219 789 617 261
BsaAI yAC'GTr
Cuts at: 493 1204 2542 4755 493
Size: 711 1338 2213 2702
Fragments arranged by size:
2702 2213 1338 711
BsaBI GATnn'nnATC
Cuts at: 6958 6958
Size: 6964
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2598 3279 3938 4771
Size: 53 83 186 936 1065 681 659 833
Cuts at: 4771 5153 5788 275
Size: 382 635 1451
Fragments arranged by size:
1451 1065 936 833 681 659 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2496 5397 5795 6069 6216 6627 2496
Size: 2901 398 274 147 411 2833
Fragments arranged by size:
2901 2833 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2435 2465 2567 2597 2657
Size: 30 165 30 1032 30 102 30 60
Cuts at: 2657 2687 3094 3124 3474 3504 1178
Size: 30 407 30 350 30 4638
Fragments arranged by size:
4638 1032 407 350 165 102 60 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2678 4148 1186
Size: 1492 1470 4002
Fragments arranged by size:
4002 1492 1470
BseYI C'CCAG_C
Cuts at: 2735 2819 2824 2953 3086 3152 3162 3176 3401
Size: 84 5 129 133 66 10 14 225
Cuts at: 3401 3443 3485 3518 3533 3750 3793 6167 6663
Size: 42 42 33 15 217 43 2374 496
Cuts at: 6663 2735
Size: 3036
Fragments arranged by size:
3036 2374 496 225 217 133 129 84
66 43 42 42 33 15 14 10
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2477 3677 1163
Size: 573 741 1200 4450
Fragments arranged by size:
4450 1200 741 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4008 4414 5175 6203 1540
Size: 275 186 2007 406 761 1028 2301
Fragments arranged by size:
2301 2007 1028 761 406 275 186
BsiHKAI G_wGCw'C
Cuts at: 3011 3629 5025 5110 6181 3011
Size: 618 1396 85 1071 3794
Fragments arranged by size:
3794 1396 1071 618 85
BsmI GAATG_Cn'
Cuts at: 4475 4568 4475
Size: 93 6871
BsmBI CGTCTCn'nnnn_
Cuts at: 2252 2252
Size: 6964
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3011 3061 3068 3104 3153 3374 3565
Size: 399 1221 50 7 36 49 221 191
Cuts at: 3565 3629 3815 3822 4046 4140 4172 4283 5025
Size: 64 186 7 224 94 32 111 742
Cuts at: 5025 5110 6181 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1221 1071 742 399 224 221 191
186 111 94 85 64 50 49 36
32 7 7
BspEI T'CCGG_A
Cuts at: 6627 6627
Size: 6964
BspHI T'CATG_A
Cuts at: 4853 6583 4853
Size: 1730 5234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2407 4319 844
Size: 1563 1912 3489
Fragments arranged by size:
3489 1912 1563
BsrBI CCG'CTC
Cuts at: 1394 1823 4851 1394
Size: 429 3028 3507
Fragments arranged by size:
3507 3028 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5459 5633 65
Size: 1183 4211 174 1396
Fragments arranged by size:
4211 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3251 3557 5608 5795 1700
Size: 110 1441 306 2051 187 2869
Fragments arranged by size:
2869 2051 1441 306 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6964
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6964
BssSI C'ACGA_G
Cuts at: 4130 5024 6036 4130
Size: 894 1012 5058
Fragments arranged by size:
5058 1012 894
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3560 3877 794
Size: 2766 317 3881
Fragments arranged by size:
3881 2766 317
BstBI TT'CG_AA
Cuts at: 5768 5768
Size: 6964
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6964
BstXI CCAn_nnnn'nTGG
Cuts at: 3493 3493
Size: 6964
BstYI r'GATC_y
Cuts at: 1972 2347 2760 4294 4661 5046 5063 6515 6601
Size: 375 413 1534 367 385 17 1452 86
Cuts at: 6601 6613 6959 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1534 1452 413 385 375 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2838 3639 3852 513
Size: 552 939 834 801 213 3625
Fragments arranged by size:
3625 939 834 801 552 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6225
Fragments arranged by size:
6225 683 56
BtsI GCAGTG_nn'
Cuts at: 3253 3343 3500 4480 5274 5294 3253
Size: 90 157 980 794 20 4923
Fragments arranged by size:
4923 980 794 157 90 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6964
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6929
DraI TTT'AAA
Cuts at: 4268 4268
Size: 6964
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2888 5971 809
Size: 507 1572 3083 1802
Fragments arranged by size:
3083 1802 1572 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2377 4411 5300
Size: 54 1425 50 275 18 547 2034 889
Cuts at: 5300 6929 8
Size: 1629 43
Fragments arranged by size:
2034 1629 1425 889 547 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4411 1537
Size: 275 2599 4090
Fragments arranged by size:
4090 2599 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2364 2858 3852 4022 4894 1353
Size: 1011 494 994 170 872 3423
Fragments arranged by size:
3423 1011 994 872 494 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3213 3234 3659 5551 5925
Size: 1064 138 435 1189 21 425 1892 374
Cuts at: 5925 6071 387
Size: 146 1280
Fragments arranged by size:
1892 1280 1189 1064 435 425 374 146
138 21
EcoICRI GAG'CTC
Cuts at: 3627 3627
Size: 6964
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2785 2871 2886 3523 4058 4244 4265
Size: 393 1153 86 15 637 535 186 21
Cuts at: 4265 5027 5624 6411 6679 1239
Size: 762 597 787 268 1524
Fragments arranged by size:
1524 1153 787 762 637 597 535 393
268 186 86 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2051 2150 3192 3827 2051
Size: 99 1042 635 5188
Fragments arranged by size:
5188 1042 635 99
EcoO109I rG'GnC_Cy
Cuts at: 2323 2650 3031 3065 3371 3482 3610 3676 3812
Size: 327 381 34 306 111 128 66 136
Cuts at: 3812 4072 4255 2323
Size: 260 183 5032
Fragments arranged by size:
5032 381 327 306 260 183 136 128
111 66 34
EcoRI G'AATT_C
Cuts at: 3986 4273 4651 3986
Size: 287 378 6299
Fragments arranged by size:
6299 378 287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4237 4237
Size: 6964
FspI TGC'GCA
Cuts at: 2546 2567 5470 2546
Size: 21 2903 4040
Fragments arranged by size:
4040 2903 21
FspAI rTGC'GCAy
Cuts at: 2546 2546
Size: 6964
HaeII r_GCGC'y
Cuts at: 1678 2717 3282 4000 6111 1678
Size: 1039 565 718 2111 2531
Fragments arranged by size:
2531 2111 1039 718 565
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2435 2467 2567 2599 3474
Size: 32 135 32 863 32 100 32 875
Cuts at: 3474 3506 3699 3731 5605 5637 5679 5711 1373
Size: 32 193 32 1874 32 42 32 2626
Fragments arranged by size:
2626 1874 875 863 193 135 100 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2596 2638 4005 4308 4554 669
Size: 988 281 658 42 1367 303 246 3079
Fragments arranged by size:
3079 1367 988 658 303 281 246 42
HindIII A'AGCT_T
Cuts at: 748 2307 748
Size: 1559 5405
HpaI GTT'AAC
Cuts at: 4554 4554
Size: 6964
KasI G'GCGC_C
Cuts at: 3278 3278
Size: 6964
KpnI G_GTAC'C
Cuts at: 4289 4289
Size: 6964
MfeI C'AATT_G
Cuts at: 4563 4563
Size: 6964
MluI A'CGCG_T
Cuts at: 6840 6840
Size: 6964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2232 3959 6078 6262 1305
Size: 927 1727 2119 184 2007
Fragments arranged by size:
2119 2007 1727 927 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2379 10
Size: 54 1425 343 547 4595
Fragments arranged by size:
4595 1425 547 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 4922 5281 5440 518
Size: 648 1251 2505 359 159 2042
Fragments arranged by size:
2505 2042 1251 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2957 3213 3766 3787 4207 4380 5057 6205
Size: 1351 256 553 21 420 173 677 1148
Cuts at: 6205 6450 1606
Size: 245 2120
Fragments arranged by size:
2120 1351 1148 677 553 420 256 245
173 21
NarI GG'CG_CC
Cuts at: 3279 3279
Size: 6964
NcoI C'CATG_G
Cuts at: 513 1065 2004 3639 3852 513
Size: 552 939 1635 213 3625
Fragments arranged by size:
3625 1635 939 552 213
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6964
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6964
NotI GC'GGCC_GC
Cuts at: 4411 4411
Size: 6964
NspI r_CATG'y
Cuts at: 1132 1229 2416 2551 2572 3880 4322 5867 1132
Size: 97 1187 135 21 1308 442 1545 2229
Fragments arranged by size:
2229 1545 1308 1187 442 135 97 21
PasI CC'CwG_GG
Cuts at: 1348 3061 1348
Size: 1713 5251
PciI A'CATG_T
Cuts at: 5863 5863
Size: 6964
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3127 3367 1108
Size: 2019 240 4705
Fragments arranged by size:
4705 2019 240
PmeI GTTT'AAAC
Cuts at: 4268 4268
Size: 6964
PmlI CAC'GTG
Cuts at: 2542 2542
Size: 6964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2857 2889 5033 5065 6546 6578 2857
Size: 32 2144 32 1481 32 3243
Fragments arranged by size:
3243 2144 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2650 3031 3482 3676 4255 2650
Size: 381 451 194 579 5359
Fragments arranged by size:
5359 579 451 381 194
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6964
PsiI TTA'TAA
Cuts at: 4534 4534
Size: 6964
PspOMI G'GGCC_C
Cuts at: 3064 3149 3370 3811 3064
Size: 85 221 441 6217
Fragments arranged by size:
6217 441 221 85
PspXI vC'TCGA_Gb
Cuts at: 2696 2696
Size: 6964
PstI C_TGCA'G
Cuts at: 830 3765 4316 830
Size: 2935 551 3478
Fragments arranged by size:
3478 2935 551
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6964
PvuII CAG'CTG
Cuts at: 2957 3766 2957
Size: 809 6155
SacI G_AGCT'C
Cuts at: 3629 3629
Size: 6964
SalI G'TCGA_C
Cuts at: 1936 4003 4306 1936
Size: 2067 303 4594
Fragments arranged by size:
4594 2067 303
SanDI GG'GwC_CC
Cuts at: 3031 3482 3031
Size: 451 6513
SbfI CC_TGCA'GG
Cuts at: 4316 4316
Size: 6964
ScaI AGT'ACT
Cuts at: 2630 5212 6924 2630
Size: 2582 1712 2670
Fragments arranged by size:
2670 2582 1712
SfcI C'TryA_G
Cuts at: 826 1045 3761 4312 5447 6128 6319 826
Size: 219 2716 551 1135 681 191 1471
Fragments arranged by size:
2716 1471 1135 681 551 219 191
SfoI GGC'GCC
Cuts at: 3280 3280
Size: 6964
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6964
SmaI CCC'GGG
Cuts at: 1872 4291 1872
Size: 2419 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2048 2696 3004 5068 5969 6231
Size: 197 926 105 648 308 2064 901 262
Cuts at: 6231 820
Size: 1553
Fragments arranged by size:
2064 1553 926 901 648 308 262 197
105
SnaBI TAC'GTA
Cuts at: 493 4755 493
Size: 4262 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6964
SphI G_CATG'C
Cuts at: 1132 2551 2572 4322 1132
Size: 1419 21 1750 3774
Fragments arranged by size:
3774 1750 1419 21
SspI AAT'ATT
Cuts at: 5 52 4888 5
Size: 47 4836 2081
Fragments arranged by size:
4836 2081 47
StuI AGG'CCT
Cuts at: 1517 3365 1517
Size: 1848 5116
StyI C'CwwG_G
Cuts at: 513 1065 2004 2026 3069 3366 3549 3639 3828
Size: 552 939 22 1043 297 183 90 189
Cuts at: 3828 3852 4068 4403 513
Size: 24 216 335 3074
Fragments arranged by size:
3074 1043 939 552 335 297 216 189
183 90 24 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3716 3785 4022 4991 5008 5161 5346
Size: 1255 2136 69 237 969 17 153 185
Cuts at: 5346 5791 325
Size: 445 1498
Fragments arranged by size:
2136 1498 1255 969 445 237 185 153
69 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2628 3725 4107
Size: 274 80 33 51 1443 651 1097 382
Cuts at: 4107 5210 6922 96
Size: 1103 1712 138
Fragments arranged by size:
1712 1443 1103 1097 651 382 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2933 2965 3094 3126 6924 6956 2933
Size: 32 129 32 3798 32 2941
Fragments arranged by size:
3798 2941 129 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6964
XbaI T'CTAG_A
Cuts at: 4300 4300
Size: 6964
XhoI C'TCGA_G
Cuts at: 1943 2696 1943
Size: 753 6211
XmaI C'CCGG_G
Cuts at: 1870 4289 1870
Size: 2419 4545
XmnI GAAnn'nnTTC
Cuts at: 5093 5093
Size: 6964
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4772 276
Size: 53 83 186 936 3238 2468
Fragments arranged by size:
3238 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoRV FseI NaeI NgoMIV NruI NsiI
PacI PfoI PsrI RsrII SacII SapI SexAI SfiI
SrfI SwaI XcmI