Restriction map for
pFN21AA0683
(Circular) (Six-base) MAPSORT of: pFN21AA0683.seq Check: 8938 from: 1 to: 7216
pFN21AA0683 7216 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2995 2995
Size: 7216
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5026 278
Size: 53 83 186 936 3490 2468
Fragments arranged by size:
3490 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3630 4559 1937
Size: 1693 929 4594
Fragments arranged by size:
4594 1693 929
Acc65I G'GTAC_C
Cuts at: 2707 4537 2707
Size: 1830 5386
AclI AA'CG_TT
Cuts at: 1563 5343 5716 1563
Size: 3780 373 3063
Fragments arranged by size:
3780 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 3032 3542 4100 5279 6663 6931 1632
Size: 497 903 510 558 1179 1384 268 1917
Fragments arranged by size:
1917 1384 1179 903 558 510 497 268
AfeI AGC'GCT
Cuts at: 2142 2724 3593 4121 2142
Size: 582 869 528 5237
Fragments arranged by size:
5237 869 582 528
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7019
AflIII A'CryG_T
Cuts at: 1203 3501 6115 7092 1203
Size: 2298 2614 977 1327
Fragments arranged by size:
2614 2298 1327 977
AgeI A'CCGG_T
Cuts at: 6047 6047
Size: 7216
AhdI GACnn_n'nnGTC
Cuts at: 2036 5945 2036
Size: 3909 3307
AleI CACnn'nnGTG
Cuts at: 1166 4229 1166
Size: 3063 4153
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2531 2759 4271 1184
Size: 1347 228 1512 4129
Fragments arranged by size:
4129 1512 1347 228
AlwNI CAG_nnn'CTG
Cuts at: 2956 3010 3871 4312 4959 6531 2956
Size: 54 861 441 647 1572 3641
Fragments arranged by size:
3641 1572 861 647 441 54
ApaI G_GGCC'C
Cuts at: 2288 2342 3616 4058 4204 4276 4465 2288
Size: 54 1274 442 146 72 189 5039
Fragments arranged by size:
5039 1274 442 189 146 72 54
ApaLI G'TGCA_C
Cuts at: 5273 6429 5273
Size: 1156 6060
ApoI r'AATT_y
Cuts at: 1493 3278 4262 4525 4751 4903 7012 1493
Size: 1785 984 263 226 152 2109 1697
Fragments arranged by size:
2109 1785 1697 984 263 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5770 160
Size: 872 4738 1606
Fragments arranged by size:
4738 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7216
AvaI C'yCGr_G
Cuts at: 1870 1943 2146 3116 3260 3308 3651 3890 4045
Size: 73 203 970 144 48 343 239 155
Cuts at: 4045 4541 1870
Size: 496 4545
Fragments arranged by size:
4545 970 496 343 239 203 155 144
73 48
BamHI G'GATC_C
Cuts at: 3252 4546 4913 3252
Size: 1294 367 5555
Fragments arranged by size:
5555 1294 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2707 2763 2988 3350 3885
Size: 325 217 627 920 56 225 362 535
Cuts at: 3885 4537 5992 618
Size: 652 1455 1842
Fragments arranged by size:
1842 1455 920 652 627 535 362 325
225 217 56
BanII G_rGCy'C
Cuts at: 1391 2219 2288 2342 3396 3458 3616 3641 3742
Size: 828 69 54 1054 62 158 25 101
Cuts at: 3742 4058 4204 4276 4465 4535 1391
Size: 316 146 72 189 70 4072
Fragments arranged by size:
4072 1054 828 316 189 158 146 101
72 70 69 62 54 25
BbeI G_GCGC'C
Cuts at: 2767 2992 3354 2767
Size: 225 362 6629
Fragments arranged by size:
6629 362 225
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2058 3707 4167 928
Size: 971 159 1649 460 3977
Fragments arranged by size:
3977 1649 971 460 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2315 2349 2926 2960 3355 3389 5407
Size: 34 378 34 577 34 395 34 2018
Cuts at: 5407 5441 1903
Size: 34 3678
Fragments arranged by size:
3678 2018 577 395 378 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5108 6318 5108
Size: 1210 6006
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7216
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2568 2798 2961
Size: 107 122 71 631 605 896 230 163
Cuts at: 2961 3359 3611 3746 3776 4062 5827 136
Size: 398 252 135 30 286 1765 1525
Fragments arranged by size:
1765 1525 896 631 605 398 286 252
230 163 135 122 107 71 30
BglII A'GATC_T
Cuts at: 7211 7211
Size: 7216
BlpI GC'TnA_GC
Cuts at: 2213 4340 4633 2213
Size: 2127 293 4796
Fragments arranged by size:
4796 2127 293
Bme1580I G_kGCm'C
Cuts at: 1790 2288 2342 3250 3368 3616 4058 4204 4276
Size: 498 54 908 118 248 442 146 72
Cuts at: 4276 4465 5277 6433 1790
Size: 189 812 1156 2573
Fragments arranged by size:
2573 1156 908 812 498 442 248 189
146 118 72 54
BmgBI CAC'GTC
Cuts at: 1318 3030 3082 3369 3576 4414 1318
Size: 1712 52 287 207 838 4120
Fragments arranged by size:
4120 1712 838 287 207 52
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2840 2884 4947
Size: 416 53 83 399 24 1423 44 2063
Cuts at: 4947 5905 442
Size: 958 1753
Fragments arranged by size:
2063 1753 1423 958 416 399 83 53
44 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7216
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 7216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 2121 2249 2273 2300 3713 4385 5876
Size: 881 1 128 24 27 1413 672 1491
Cuts at: 5876 1239
Size: 2579
Fragments arranged by size:
2579 1491 1413 881 672 128 27 24
1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3638 4469 5341 6206 6504 3638
Size: 831 872 865 298 4350
Fragments arranged by size:
4350 872 865 831 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2312 3398 5879 882
Size: 617 813 1086 2481 2219
Fragments arranged by size:
2481 2219 1086 813 617
BsaAI yAC'GTr
Cuts at: 493 1204 4300 5007 493
Size: 711 3096 707 2702
Fragments arranged by size:
3096 2702 711 707
BsaBI GATnn'nnATC
Cuts at: 7210 7210
Size: 7216
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2764 2939 2989 3214
Size: 53 83 186 936 1231 175 50 225
Cuts at: 3214 3351 3432 3977 5023 5405 6040 275
Size: 137 81 545 1046 382 635 1451
Fragments arranged by size:
1451 1231 1046 936 635 545 382 225
186 175 137 83 81 53 50
BsaWI w'CCGG_w
Cuts at: 5649 6047 6321 6468 6879 5649
Size: 398 274 147 411 5986
Fragments arranged by size:
5986 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2246 2276 2408 2438 1178
Size: 30 165 30 843 30 132 30 5956
Fragments arranged by size:
5956 843 165 132 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2270 2588 3710 4016 4318 4487 1186
Size: 988 96 318 1122 306 302 169 3915
Fragments arranged by size:
3915 1122 988 318 306 302 169 96
BseYI C'CCAG_C
Cuts at: 2218 2342 2473 2857 2959 3333 3594 4023 4070
Size: 124 131 384 102 374 261 429 47
Cuts at: 4070 6419 6915 2218
Size: 2349 496 2519
Fragments arranged by size:
2519 2349 496 429 384 374 261 131
124 102 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2077 2729 1163
Size: 573 341 652 5650
Fragments arranged by size:
5650 652 573 341
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4323 4666 5427 6455 1540
Size: 275 186 2322 343 761 1028 2301
Fragments arranged by size:
2322 2301 1028 761 343 275 186
BsiHKAI G_wGCw'C
Cuts at: 5277 5362 6433 5277
Size: 85 1071 6060
Fragments arranged by size:
6060 1071 85
BsmI GAATG_Cn'
Cuts at: 4727 4820 4727
Size: 93 7123
BsmBI CGTCTCn'nnnn_
Cuts at: 2459 3020 3958 4330 4515 2459
Size: 561 938 372 185 5160
Fragments arranged by size:
5160 938 561 372 185
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2219 2288 2342 3250 3368 3396 3458
Size: 399 429 69 54 908 118 28 62
Cuts at: 3458 3616 3641 3742 4058 4204 4276 4465 4535
Size: 158 25 101 316 146 72 189 70
Cuts at: 4535 5277 5362 6433 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 908 742 429 399 316 189
158 146 118 101 85 72 70 69
62 54 28 25
BspEI T'CCGG_A
Cuts at: 6879 6879
Size: 7216
BspHI T'CATG_A
Cuts at: 5105 6835 5105
Size: 1730 5486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2751 2995 4571 844
Size: 1907 244 1576 3489
Fragments arranged by size:
3489 1907 1576 244
BsrBI CCG'CTC
Cuts at: 1394 1823 2995 4006 5103 1394
Size: 429 1172 1011 1097 3507
Fragments arranged by size:
3507 1172 1097 1011 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5711 5885 65
Size: 1183 4463 174 1396
Fragments arranged by size:
4463 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2397 4091 5860 6047 1700
Size: 110 587 1694 1769 187 2869
Fragments arranged by size:
2869 1769 1694 587 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7216
BssHII G'CGCG_C
Cuts at: 1925 4355 1925
Size: 2430 4786
BssSI C'ACGA_G
Cuts at: 5276 6288 5276
Size: 1012 6204
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2956 794
Size: 2162 5054
BstBI TT'CG_AA
Cuts at: 6020 6020
Size: 7216
BstEII G'GTnAC_C
Cuts at: 1184 4138 1184
Size: 2954 4262
BstXI CCAn_nnnn'nTGG
Cuts at: 3166 3166
Size: 7216
BstYI r'GATC_y
Cuts at: 1972 2691 3252 4546 4913 5298 5315 6767 6853
Size: 719 561 1294 367 385 17 1452 86
Cuts at: 6853 6865 7211 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1294 719 561 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2838 4195 2838
Size: 1357 5859
BtgI C'CryG_G
Cuts at: 513 1065 2004 2242 2674 2793 2973 3384 3972
Size: 552 939 238 432 119 180 411 588
Cuts at: 3972 4086 4227 4242 513
Size: 114 141 15 3487
Fragments arranged by size:
3487 939 588 552 432 411 238 180
141 119 114 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3492 487
Size: 683 56 2266 4211
Fragments arranged by size:
4211 2266 683 56
BtsI GCAGTG_nn'
Cuts at: 2412 2429 3049 3973 4732 5526 5546 2412
Size: 17 620 924 759 794 20 4082
Fragments arranged by size:
4082 924 794 759 620 20 17
ClaI AT'CG_AT
Cuts at: 1554 4290 1554
Size: 2736 4480
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7181
DraI TTT'AAA
Cuts at: 4520 4520
Size: 7216
DraIII CAC_nnn'GTG
Cuts at: 3933 3933
Size: 7216
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6223 809
Size: 507 4907 1802
Fragments arranged by size:
4907 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 2245 2376
Size: 54 1425 50 275 18 250 165 131
Cuts at: 2376 2532 2970 3156 3672 3747 3777 4089 4093
Size: 156 438 186 516 75 30 312 4
Cuts at: 4093 4239 4320 4663 5552 7181 8
Size: 146 81 343 889 1629 43
Fragments arranged by size:
1629 1425 889 516 438 343 312 275
250 186 165 156 146 131 81 75
54 50 43 30 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 4320 4663 1537
Size: 275 2508 343 4090
Fragments arranged by size:
4090 2508 343 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3285 3518 5146 1353
Size: 1932 233 1628 3423
Fragments arranged by size:
3423 1932 1628 233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2306 2549 3041 4424 5803 6177
Size: 1064 138 717 243 492 1383 1379 374
Cuts at: 6177 6323 387
Size: 146 1280
Fragments arranged by size:
1383 1379 1280 1064 717 492 374 243
146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2121 2129 2249 2273 2300 3032
Size: 393 488 1 8 120 24 27 732
Cuts at: 3032 3542 3713 4100 4385 5279 5876 6663 6931
Size: 510 171 387 285 894 597 787 268
Cuts at: 6931 1239
Size: 1524
Fragments arranged by size:
1524 894 787 732 597 510 488 393
387 285 268 171 120 27 24 8
1
EcoNI CCTnn'n_nnAGG
Cuts at: 2390 2519 3002 3941 4364 2390
Size: 129 483 939 423 5242
Fragments arranged by size:
5242 939 483 423 129
EcoO109I rG'GnC_Cy
Cuts at: 2284 2339 2653 2834 2842 3266 3613 3622 4054
Size: 55 314 181 8 424 347 9 432
Cuts at: 4054 4200 4201 4252 4273 4369 4461 4462 2284
Size: 146 1 51 21 96 92 1 5038
Fragments arranged by size:
5038 432 424 347 314 181 146 96
92 55 51 21 9 8 1 1
EcoRI G'AATT_C
Cuts at: 4262 4525 4903 4262
Size: 263 378 6575
Fragments arranged by size:
6575 378 263
FseI GG_CCGG'CC
Cuts at: 4095 4095
Size: 7216
FspI TGC'GCA
Cuts at: 5722 5722
Size: 7216
HaeII r_GCGC'y
Cuts at: 1678 2144 2726 2767 2992 3079 3354 3595 4123
Size: 466 582 41 225 87 275 241 528
Cuts at: 4123 6363 1678
Size: 2240 2531
Fragments arranged by size:
2531 2240 582 528 466 275 241 225
87 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2022 2054 2119 2151 3338
Size: 32 135 32 450 32 65 32 1187
Cuts at: 3338 3370 5857 5889 5931 5963 1373
Size: 32 2487 32 42 32 2626
Fragments arranged by size:
2626 2487 1187 450 135 65 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4222 4560 4806 669
Size: 988 281 2284 338 246 3079
Fragments arranged by size:
3079 2284 988 338 281 246
HindIII A'AGCT_T
Cuts at: 748 2909 748
Size: 2161 5055
HpaI GTT'AAC
Cuts at: 4222 4806 4222
Size: 584 6632
KasI G'GCGC_C
Cuts at: 2763 2988 3350 2763
Size: 225 362 6629
Fragments arranged by size:
6629 362 225
KpnI G_GTAC'C
Cuts at: 2711 4541 2711
Size: 1830 5386
MfeI C'AATT_G
Cuts at: 4815 4815
Size: 7216
MluI A'CGCG_T
Cuts at: 7092 7092
Size: 7216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3003 6330 6514 1305
Size: 1698 3327 184 2007
Fragments arranged by size:
3327 2007 1698 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 2972 3158 3674 4241
Size: 54 1425 343 546 594 186 516 567
Cuts at: 4241 10
Size: 2985
Fragments arranged by size:
2985 1425 594 567 546 516 343 186
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3227 4229 4290 5174 5533 5692 518
Size: 648 2061 1002 61 884 359 159 2042
Fragments arranged by size:
2061 2042 1002 884 648 359 159 61
MspA1I CmG'CkG
Cuts at: 1606 2986 3448 4027 4168 4632 5309 6457 6702
Size: 1380 462 579 141 464 677 1148 245
Cuts at: 6702 1606
Size: 2120
Fragments arranged by size:
2120 1380 1148 677 579 464 462 245
141
NaeI GCC'GGC
Cuts at: 2399 4093 2399
Size: 1694 5522
NarI GG'CG_CC
Cuts at: 2764 2989 3351 2764
Size: 225 362 6629
Fragments arranged by size:
6629 362 225
NcoI C'CATG_G
Cuts at: 513 1065 2004 2242 2793 2973 4242 513
Size: 552 939 238 551 180 1269 3487
Fragments arranged by size:
3487 1269 939 552 551 238 180
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7216
NgoMIV G'CCGG_C
Cuts at: 2397 4091 2397
Size: 1694 5522
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7216
NotI GC'GGCC_GC
Cuts at: 4663 4663
Size: 7216
NspI r_CATG'y
Cuts at: 1132 1229 3505 4574 6119 1132
Size: 97 2276 1069 1545 2229
Fragments arranged by size:
2276 2229 1545 1069 97
PasI CC'CwG_GG
Cuts at: 1348 2731 1348
Size: 1383 5833
PciI A'CATG_T
Cuts at: 3501 6115 3501
Size: 2614 4602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7216
PfoI T'CCnGG_A
Cuts at: 2040 2277 2460 3549 3840 2040
Size: 237 183 1089 291 5416
Fragments arranged by size:
5416 1089 291 237 183
PmeI GTTT'AAAC
Cuts at: 4520 4520
Size: 7216
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3291 3323 5285 5317 6798 6830 3291
Size: 32 1962 32 1481 32 3677
Fragments arranged by size:
3677 1962 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2653 2842 4369 2653
Size: 189 1527 5500
Fragments arranged by size:
5500 1527 189
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7216
PsiI TTA'TAA
Cuts at: 4786 4786
Size: 7216
PspOMI G'GGCC_C
Cuts at: 2284 2338 3612 4054 4200 4272 4461 2284
Size: 54 1274 442 146 72 189 5039
Fragments arranged by size:
5039 1274 442 189 146 72 54
PspXI vC'TCGA_Gb
Cuts at: 3308 3308
Size: 7216
PstI C_TGCA'G
Cuts at: 830 2520 2595 2952 3006 3403 4445 4479 4568
Size: 1690 75 357 54 397 1042 34 89
Cuts at: 4568 830
Size: 3478
Fragments arranged by size:
3478 1690 1042 397 357 89 75 54
34
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7216
PvuII CAG'CTG
Cuts at: 3448 4168 3448
Size: 720 6496
RsrII CG'GwC_CG
Cuts at: 2785 2785
Size: 7216
SalI G'TCGA_C
Cuts at: 1936 4558 1936
Size: 2622 4594
SapI GCTCTTCn'nnn_
Cuts at: 3518 3518
Size: 7216
SbfI CC_TGCA'GG
Cuts at: 4568 4568
Size: 7216
ScaI AGT'ACT
Cuts at: 5464 7176 5464
Size: 1712 5504
SexAI A'CCwGG_T
Cuts at: 2865 2865
Size: 7216
SfcI C'TryA_G
Cuts at: 826 1045 2516 2591 2948 3002 3399 3631 3814
Size: 219 1471 75 357 54 397 232 183
Cuts at: 3814 4441 4475 4564 5699 6380 6571 826
Size: 627 34 89 1135 681 191 1471
Fragments arranged by size:
1471 1471 1135 681 627 397 357 232
219 191 183 89 75 54 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 3776 3776
Size: 7216
SfoI GGC'GCC
Cuts at: 2765 2990 3352 2765
Size: 225 362 6629
Fragments arranged by size:
6629 362 225
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7216
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7216
SmaI CCC'GGG
Cuts at: 1872 4543 1872
Size: 2671 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3308 3617 4484 5320 6221 6483
Size: 197 926 1365 309 867 836 901 262
Cuts at: 6483 820
Size: 1553
Fragments arranged by size:
1553 1365 926 901 867 836 309 262
197
SnaBI TAC'GTA
Cuts at: 493 5007 493
Size: 4514 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7216
SphI G_CATG'C
Cuts at: 1132 4574 1132
Size: 3442 3774
SspI AAT'ATT
Cuts at: 5 52 5140 5
Size: 47 5088 2081
Fragments arranged by size:
5088 2081 47
StuI AGG'CCT
Cuts at: 1517 3880 4132 1517
Size: 2363 252 4601
Fragments arranged by size:
4601 2363 252
StyI C'CwwG_G
Cuts at: 513 1065 2004 2242 2614 2656 2793 2973 3321
Size: 552 939 238 372 42 137 180 348
Cuts at: 3321 3675 4035 4242 4655 513
Size: 354 360 207 413 3074
Fragments arranged by size:
3074 939 552 413 372 360 354 348
238 207 180 137 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2744 3873 5243 5260 5413 5598 6043
Size: 1255 1164 1129 1370 17 153 185 445
Cuts at: 6043 325
Size: 1498
Fragments arranged by size:
1498 1370 1255 1164 1129 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5462 7174 96
Size: 274 80 33 51 1443 3485 1712 138
Fragments arranged by size:
3485 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2246 2278 2408 2440 2653 2685 4253 4285 7176
Size: 32 130 32 213 32 1568 32 2891
Cuts at: 7176 7208 2246
Size: 32 2254
Fragments arranged by size:
2891 2254 1568 213 130 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3574 1316
Size: 2258 4958
XbaI T'CTAG_A
Cuts at: 4552 4552
Size: 7216
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2275 4234 2275
Size: 1959 5257
XhoI C'TCGA_G
Cuts at: 1943 3308 1943
Size: 1365 5851
XmaI C'CCGG_G
Cuts at: 1870 4541 1870
Size: 2671 4545
XmnI GAAnn'nnTTC
Cuts at: 2049 2555 5345 2049
Size: 506 2790 3920
Fragments arranged by size:
3920 2790 506
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5024 276
Size: 53 83 186 936 3490 2468
Fragments arranged by size:
3490 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I EcoICRI EcoRV FalI FspAI NruI NsiI PacI
PmlI PsrI SacI SacII SanDI SrfI SwaI