Restriction map for
pFN21AA0676
(Circular) (Six-base) MAPSORT of: pFN21AA0676.seq Check: 4413 from: 1 to: 8455
pFN21AA0676 8455 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4096 4096
Size: 8455
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6265 278
Size: 53 83 186 936 4729 2468
Fragments arranged by size:
4729 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2214 4068 5580 5798 1937
Size: 277 1854 1512 218 4594
Fragments arranged by size:
4594 1854 1512 277 218
Acc65I G'GTAC_C
Cuts at: 5776 5776
Size: 8455
AclI AA'CG_TT
Cuts at: 1563 6582 6955 1563
Size: 5019 373 3063
Fragments arranged by size:
5019 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2450 2855 3392 3542 5285 6518 7902 8170
Size: 818 405 537 150 1743 1233 1384 268
Cuts at: 8170 1632
Size: 1917
Fragments arranged by size:
1917 1743 1384 1233 818 537 405 268
150
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8258
AflIII A'CryG_T
Cuts at: 1203 2612 5112 5457 7354 8331 1203
Size: 1409 2500 345 1897 977 1327
Fragments arranged by size:
2500 1897 1409 1327 977 345
AgeI A'CCGG_T
Cuts at: 7286 7286
Size: 8455
AhdI GACnn_n'nnGTC
Cuts at: 5458 7184 5458
Size: 1726 6729
AleI CACnn'nnGTG
Cuts at: 1166 3896 4245 1166
Size: 2730 349 5376
Fragments arranged by size:
5376 2730 349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4263 1184
Size: 3079 5376
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5696 5728 5696
Size: 32 8423
AlwNI CAG_nnn'CTG
Cuts at: 2296 2496 2761 3367 3982 4147 4375 5143 5238
Size: 200 265 606 615 165 228 768 95
Cuts at: 5238 5431 5614 6198 7770 2296
Size: 193 183 584 1572 2981
Fragments arranged by size:
2981 1572 768 615 606 584 265 228
200 193 183 165 95
ApaLI G'TGCA_C
Cuts at: 6512 7668 6512
Size: 1156 7299
ApoI r'AATT_y
Cuts at: 1493 4322 5764 5990 6142 8251 1493
Size: 2829 1442 226 152 2109 1697
Fragments arranged by size:
2829 2109 1697 1442 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7009 160
Size: 872 5977 1606
Fragments arranged by size:
5977 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8455
AvaI C'yCGr_G
Cuts at: 1870 1943 2405 2645 2853 3599 3689 5780 1870
Size: 73 462 240 208 746 90 2091 4545
Fragments arranged by size:
4545 2091 746 462 240 208 90 73
AvrII C'CTAG_G
Cuts at: 2798 2798
Size: 8455
BamHI G'GATC_C
Cuts at: 5785 6152 5785
Size: 367 8088
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2135 3346 5573 5776 7231
Size: 325 217 627 348 1211 2227 203 1455
Cuts at: 7231 618
Size: 1842
Fragments arranged by size:
2227 1842 1455 1211 627 348 325 217
203
BanII G_rGCy'C
Cuts at: 1391 2018 2682 3910 3939 4302 4805 5417 5495
Size: 627 664 1228 29 363 503 612 78
Cuts at: 5495 5774 1391
Size: 279 4072
Fragments arranged by size:
4072 1228 664 627 612 503 363 279
78 29
BbeI G_GCGC'C
Cuts at: 3350 3350
Size: 8455
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2117 3252 4350 5130 5514 928
Size: 971 218 1135 1098 780 384 3869
Fragments arranged by size:
3869 1135 1098 971 780 384 218
BbvCI CC'TCA_GC
Cuts at: 3314 4031 3314
Size: 717 7738
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3680 3714 4991 5025 6646 6680 1903
Size: 34 1743 34 1277 34 1621 34 3678
Fragments arranged by size:
3678 1743 1621 1277 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 6347 7557 3539
Size: 2808 1210 4437
Fragments arranged by size:
4437 2808 1210
BclI T'GATC_A
Cuts at: 1548 4698 1548
Size: 3150 5305
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2171 2744 2809
Size: 107 122 71 631 605 499 573 65
Cuts at: 2809 3419 4829 5165 7066 136
Size: 610 1410 336 1901 1525
Fragments arranged by size:
1901 1525 1410 631 610 605 573 499
336 122 107 71 65
BglII A'GATC_T
Cuts at: 4885 8450 4885
Size: 3565 4890
BlpI GC'TnA_GC
Cuts at: 2012 2424 3612 4256 5872 2012
Size: 412 1188 644 1616 4595
Fragments arranged by size:
4595 1616 1188 644 412
Bme1580I G_kGCm'C
Cuts at: 1790 2138 2167 2514 3514 6516 7672 1790
Size: 348 29 347 1000 3002 1156 2573
Fragments arranged by size:
3002 2573 1156 1000 348 347 29
BmgBI CAC'GTC
Cuts at: 1318 2146 1318
Size: 828 7627
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2272 4522 6186
Size: 416 53 83 399 24 855 2250 1664
Cuts at: 6186 7144 442
Size: 958 1753
Fragments arranged by size:
2250 1753 1664 958 855 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4506 1056
Size: 3450 5005
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4242 4873 4242
Size: 631 7824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2143 2273 2639 2801 3061 3170 3979 4484
Size: 904 130 366 162 260 109 809 505
Cuts at: 4484 4586 4847 5763 7115 1239
Size: 102 261 916 1352 2579
Fragments arranged by size:
2579 1352 916 904 809 505 366 261
260 162 130 109 102
Bpu10I CC'TnA_GC
Cuts at: 3314 4031 3314
Size: 717 7738
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4748 4899 5663 6580 7445 7743 4748
Size: 151 764 917 865 298 5460
Fragments arranged by size:
5460 917 865 764 298 151
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2705 2835 3663 5313 7118 882
Size: 617 1206 130 828 1650 1805 2219
Fragments arranged by size:
2219 1805 1650 1206 828 617 130
BsaAI yAC'GTr
Cuts at: 493 1204 6246 493
Size: 711 5042 2702
Fragments arranged by size:
5042 2702 711
BsaBI GATnn'nnATC
Cuts at: 2975 4890 8449 2975
Size: 1915 3559 2981
Fragments arranged by size:
3559 2981 1915
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2089 2850 2939 3347
Size: 53 83 186 936 556 761 89 408
Cuts at: 3347 4128 6262 6644 7279 275
Size: 781 2134 382 635 1451
Fragments arranged by size:
2134 1451 936 781 761 635 556 408
382 186 89 83 53
BsaWI w'CCGG_w
Cuts at: 4575 4990 6888 7286 7560 7707 8118 4575
Size: 415 1898 398 274 147 411 4912
Fragments arranged by size:
4912 1898 415 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3897 3927 4972 5002 5696
Size: 30 165 30 2494 30 1045 30 694
Cuts at: 5696 5726 1178
Size: 30 3907
Fragments arranged by size:
3907 2494 1045 694 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 3026 3261 3455 3551 3671 3845 3848
Size: 850 990 235 194 96 120 174 3
Cuts at: 3848 4218 4426 4849 4863 4973 4980 4985 5264
Size: 370 208 423 14 110 7 5 279
Cuts at: 5264 1186
Size: 4377
Fragments arranged by size:
4377 990 850 423 370 279 235 208
194 174 120 110 96 14 7 5
3
BseYI C'CCAG_C
Cuts at: 2569 3089 3265 3301 3715 4147 4183 4550 4792
Size: 520 176 36 414 432 36 367 242
Cuts at: 4792 5308 5424 7658 8154 2569
Size: 516 116 2234 496 2870
Fragments arranged by size:
2870 2234 520 516 496 432 414 367
242 176 116 36 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 2615 2911 2939 3185 3659 4540
Size: 573 438 441 296 28 246 474 881
Cuts at: 4540 1163
Size: 5078
Fragments arranged by size:
5078 881 573 474 441 438 296 246
28
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4540 5905 6666 7694 1540
Size: 275 186 2539 1365 761 1028 2301
Fragments arranged by size:
2539 2301 1365 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2682 3696 3813 3939 4302 4773 6516 6601 7672
Size: 1014 117 126 363 471 1743 85 1071
Cuts at: 7672 2682
Size: 3465
Fragments arranged by size:
3465 1743 1071 1014 471 363 126 117
85
BsmI GAATG_Cn'
Cuts at: 5966 6059 5966
Size: 93 8362
BsmBI CGTCTCn'nnnn_
Cuts at: 2388 5039 2388
Size: 2651 5804
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2138 2167 2514 2682 3514 3696
Size: 399 228 120 29 347 168 832 182
Cuts at: 3696 3813 3910 3939 4302 4773 4805 5417 5495
Size: 117 97 29 363 471 32 612 78
Cuts at: 5495 5774 6516 6601 7672 1391
Size: 279 742 85 1071 2174
Fragments arranged by size:
2174 1071 832 742 612 471 399 363
347 279 228 182 168 120 117 97
85 78 32 29 29
BspEI T'CCGG_A
Cuts at: 4990 8118 4990
Size: 3128 5327
BspHI T'CATG_A
Cuts at: 6344 8074 6344
Size: 1730 6725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2388 3681 4096 5810 844
Size: 1544 1293 415 1714 3489
Fragments arranged by size:
3489 1714 1544 1293 415
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 2905 3681 4731 6342 1394
Size: 429 236 846 776 1050 1611 3507
Fragments arranged by size:
3507 1611 1050 846 776 429 236
BsrDI GCAATG_nn'
Cuts at: 65 1248 3795 5507 6950 7124 65
Size: 1183 2547 1712 1443 174 1396
Fragments arranged by size:
2547 1712 1443 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4535 7099 7286 1700
Size: 110 2725 2564 187 2869
Fragments arranged by size:
2869 2725 2564 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8455
BssHII G'CGCG_C
Cuts at: 1925 5561 1925
Size: 3636 4819
BssSI C'ACGA_G
Cuts at: 2129 2588 3940 6515 7527 2129
Size: 459 1352 2575 1012 3057
Fragments arranged by size:
3057 2575 1352 1012 459
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8455
BstBI TT'CG_AA
Cuts at: 3930 5011 7259 3930
Size: 1081 2248 5126
Fragments arranged by size:
5126 2248 1081
BstEII G'GTnAC_C
Cuts at: 1184 3049 1184
Size: 1865 6590
BstXI CCAn_nnnn'nTGG
Cuts at: 3241 3901 4498 3241
Size: 660 597 7198
Fragments arranged by size:
7198 660 597
BstYI r'GATC_y
Cuts at: 1972 3171 4885 4891 5056 5610 5785 6152 6537
Size: 1199 1714 6 165 554 175 367 385
Cuts at: 6537 6554 8006 8092 8104 8450 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1714 1452 1199 554 385 367 346
175 165 86 17 12 6
Bsu36I CC'TnA_GG
Cuts at: 2457 3041 3273 4334 2457
Size: 584 232 1061 6578
Fragments arranged by size:
6578 1061 584 232
BtgI C'CryG_G
Cuts at: 513 1065 2098 3396 4056 4741 5130 5163 513
Size: 552 1033 1298 660 685 389 33 3805
Fragments arranged by size:
3805 1298 1033 685 660 552 389 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2344 4281 5140 487
Size: 683 56 1118 1937 859 3802
Fragments arranged by size:
3802 1937 1118 859 683 56
BtsI GCAGTG_nn'
Cuts at: 2603 3832 5971 6765 6785 2603
Size: 1229 2139 794 20 4273
Fragments arranged by size:
4273 2139 1229 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8455
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8420
DraI TTT'AAA
Cuts at: 5759 5759
Size: 8455
DraIII CAC_nnn'GTG
Cuts at: 2934 2934
Size: 8455
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2210 7462 809
Size: 507 894 5252 1802
Fragments arranged by size:
5252 1802 894 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2736 2745
Size: 54 1425 50 275 18 204 702 9
Cuts at: 2745 2810 2914 2965 3648 4116 4404 4533 4537
Size: 65 104 51 683 468 288 129 4
Cuts at: 4537 5166 5902 6791 8420 8
Size: 629 736 889 1629 43
Fragments arranged by size:
1629 1425 889 736 702 683 629 468
288 275 204 129 104 65 54 51
50 43 18 9 4
EagI C'GGCC_G
Cuts at: 1537 1812 4537 5902 1537
Size: 275 2725 1365 4090
Fragments arranged by size:
4090 2725 1365 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2361 2645 2687 3383 3569 3927 4031 4452
Size: 1008 284 42 696 186 358 104 421
Cuts at: 4452 4929 5654 6385 1353
Size: 477 725 731 3423
Fragments arranged by size:
3423 1008 731 725 696 477 421 358
284 186 104 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5355 7042 7416 7562 387
Size: 1064 138 3766 1687 374 146 1280
Fragments arranged by size:
3766 1687 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2680 3937 4300 2680
Size: 1257 363 6835
Fragments arranged by size:
6835 1257 363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2143 2273 2450 2639 2801 2855 3061
Size: 393 511 130 177 189 162 54 206
Cuts at: 3061 3170 3392 3542 3979 4484 4586 4847 5285
Size: 109 222 150 437 505 102 261 438
Cuts at: 5285 5763 6518 7115 7902 8170 1239
Size: 478 755 597 787 268 1524
Fragments arranged by size:
1524 787 755 597 511 505 478 438
437 393 268 261 222 206 189 177
162 150 130 109 102 54
EcoNI CCTnn'n_nnAGG
Cuts at: 3044 3044
Size: 8455
EcoO109I rG'GnC_Cy
Cuts at: 2401 2791 3364 3403 3573 3979 4468 4546 4747
Size: 390 573 39 170 406 489 78 201
Cuts at: 4747 5140 2401
Size: 393 5716
Fragments arranged by size:
5716 573 489 406 393 390 201 170
78 39
EcoRI G'AATT_C
Cuts at: 5764 6142 5764
Size: 378 8077
EcoRV GAT'ATC
Cuts at: 2323 2323
Size: 8455
FseI GG_CCGG'CC
Cuts at: 4539 4539
Size: 8455
FspI TGC'GCA
Cuts at: 3339 6961 3339
Size: 3622 4833
HaeII r_GCGC'y
Cuts at: 1678 3350 4056 7602 1678
Size: 1672 706 3546 2531
Fragments arranged by size:
3546 2531 1672 706
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3577 3609 4844 4876 4972
Size: 32 135 32 2005 32 1235 32 96
Cuts at: 4972 5004 5041 5073 5444 5476 5667 5699 7096
Size: 32 37 32 371 32 191 32 1397
Cuts at: 7096 7128 7170 7202 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2005 1397 1235 371 191 135 96
42 37 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2542 4069 4210 5581 5799 6045
Size: 988 281 604 1527 141 1371 218 246
Cuts at: 6045 669
Size: 3079
Fragments arranged by size:
3079 1527 1371 988 604 281 246 218
141
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8455
HpaI GTT'AAC
Cuts at: 6045 6045
Size: 8455
KasI G'GCGC_C
Cuts at: 3346 3346
Size: 8455
KpnI G_GTAC'C
Cuts at: 5780 5780
Size: 8455
MfeI C'AATT_G
Cuts at: 6054 6054
Size: 8455
MluI A'CGCG_T
Cuts at: 2612 8331 2612
Size: 5719 2736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3006 3264 7569 7753 1305
Size: 1701 258 4305 184 2007
Fragments arranged by size:
4305 2007 1701 258 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2036 2738 2747 2967 3650
Size: 54 1425 343 204 702 9 220 683
Cuts at: 3650 5168 10
Size: 1518 3297
Fragments arranged by size:
3297 1518 1425 702 683 343 220 204
54 9
MslI CAynn'nnrTG
Cuts at: 518 1166 2657 3896 4094 4178 4245 5462 6413
Size: 648 1491 1239 198 84 67 1217 951
Cuts at: 6413 6772 6931 518
Size: 359 159 2042
Fragments arranged by size:
2042 1491 1239 1217 951 648 359 198
159 84 67
MspA1I CmG'CkG
Cuts at: 1606 2758 4144 5312 5400 5428 5871 6548 7696
Size: 1152 1386 1168 88 28 443 677 1148
Cuts at: 7696 7941 1606
Size: 245 2120
Fragments arranged by size:
2120 1386 1168 1152 1148 677 443 245
88 28
NaeI GCC'GGC
Cuts at: 4537 4537
Size: 8455
NarI GG'CG_CC
Cuts at: 3347 3347
Size: 8455
NcoI C'CATG_G
Cuts at: 513 1065 3396 5130 513
Size: 552 2331 1734 3838
Fragments arranged by size:
3838 2331 1734 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8455
NgoMIV G'CCGG_C
Cuts at: 4535 4535
Size: 8455
NheI G'CTAG_C
Cuts at: 1052 4502 1052
Size: 3450 5005
NotI GC'GGCC_GC
Cuts at: 5902 5902
Size: 8455
NspI r_CATG'y
Cuts at: 1132 1229 3490 3874 4666 5116 5461 5813 7358
Size: 97 2261 384 792 450 345 352 1545
Cuts at: 7358 1132
Size: 2229
Fragments arranged by size:
2261 2229 1545 792 450 384 352 345
97
PasI CC'CwG_GG
Cuts at: 1348 2389 2895 3361 4623 4905 5232 1348
Size: 1041 506 466 1262 282 327 4571
Fragments arranged by size:
4571 1262 1041 506 466 327 282
PciI A'CATG_T
Cuts at: 5112 5457 7354 5112
Size: 345 1897 6213
Fragments arranged by size:
6213 1897 345
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4393 5383 1108
Size: 3285 990 4180
Fragments arranged by size:
4180 3285 990
PfoI T'CCnGG_A
Cuts at: 2778 4122 4596 4674 2778
Size: 1344 474 78 6559
Fragments arranged by size:
6559 1344 474 78
PmeI GTTT'AAAC
Cuts at: 5759 5759
Size: 8455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5696 5728 6524 6556 8037 8069 5696
Size: 32 796 32 1481 32 6082
Fragments arranged by size:
6082 1481 796 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2401 3403 3979 4468 4546 4747 5140 2401
Size: 1002 576 489 78 201 393 5716
Fragments arranged by size:
5716 1002 576 489 393 201 78
PshAI GACnn'nnGTC
Cuts at: 1613 3056 1613
Size: 1443 7012
PsiI TTA'TAA
Cuts at: 6025 6025
Size: 8455
PstI C_TGCA'G
Cuts at: 830 2085 2385 2492 4107 4157 4282 5317 5552
Size: 1255 300 107 1615 50 125 1035 235
Cuts at: 5552 5807 830
Size: 255 3478
Fragments arranged by size:
3478 1615 1255 1035 300 255 235 125
107 50
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8455
PvuII CAG'CTG
Cuts at: 2758 4144 5312 5428 2758
Size: 1386 1168 116 5785
Fragments arranged by size:
5785 1386 1168 116
SacI G_AGCT'C
Cuts at: 2682 3939 4302 2682
Size: 1257 363 6835
Fragments arranged by size:
6835 1257 363
SalI G'TCGA_C
Cuts at: 1936 4067 5579 5797 1936
Size: 2131 1512 218 4594
Fragments arranged by size:
4594 2131 1512 218
SanDI GG'GwC_CC
Cuts at: 4546 4546
Size: 8455
SapI GCTCTTCn'nnn_
Cuts at: 2645 2687 3383 3927 4452 4929 2645
Size: 42 696 544 525 477 6171
Fragments arranged by size:
6171 696 544 525 477 42
SbfI CC_TGCA'GG
Cuts at: 4107 5807 4107
Size: 1700 6755
ScaI AGT'ACT
Cuts at: 4511 6703 8415 4511
Size: 2192 1712 4551
Fragments arranged by size:
4551 2192 1712
SfcI C'TryA_G
Cuts at: 826 1045 2081 2381 2488 4103 4153 4278 5313
Size: 219 1036 300 107 1615 50 125 1035
Cuts at: 5313 5548 5803 6938 7619 7810 826
Size: 235 255 1135 681 191 1471
Fragments arranged by size:
1615 1471 1135 1036 1035 681 300 255
235 219 191 125 107 50
SfiI GGCCn_nnn'nGGCC
Cuts at: 2744 2809 2744
Size: 65 8390
SfoI GGC'GCC
Cuts at: 3348 3348
Size: 8455
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8455
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8455
SmaI CCC'GGG
Cuts at: 1872 2407 3601 5782 1872
Size: 535 1194 2181 4545
Fragments arranged by size:
4545 2181 1194 535
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4763 4914 5678 6559 7460 7722
Size: 197 926 2820 151 764 881 901 262
Cuts at: 7722 820
Size: 1553
Fragments arranged by size:
2820 1553 926 901 881 764 262 197
151
SnaBI TAC'GTA
Cuts at: 493 6246 493
Size: 5753 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8455
SphI G_CATG'C
Cuts at: 1132 3874 5813 1132
Size: 2742 1939 3774
Fragments arranged by size:
3774 2742 1939
SspI AAT'ATT
Cuts at: 5 52 6379 5
Size: 47 6327 2081
Fragments arranged by size:
6327 2081 47
StuI AGG'CCT
Cuts at: 1517 3839 5546 1517
Size: 2322 1707 4426
Fragments arranged by size:
4426 2322 1707
StyI C'CwwG_G
Cuts at: 513 1065 2798 3396 3406 3414 3423 3919 4392
Size: 552 1733 598 10 8 9 496 473
Cuts at: 4392 5022 5130 5894 513
Size: 630 108 764 3074
Fragments arranged by size:
3074 1733 764 630 598 552 496 473
108 10 9 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2038 2590 6482 6499 6652 6837 7282
Size: 1255 458 552 3892 17 153 185 445
Cuts at: 7282 325
Size: 1498
Fragments arranged by size:
3892 1498 1255 552 458 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3639 4509 6701
Size: 274 80 33 51 1443 1662 870 2192
Cuts at: 6701 8413 96
Size: 1712 138
Fragments arranged by size:
2192 1712 1662 1443 870 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3220 3252 3897 3929 8415 8447 3220
Size: 32 645 32 4486 32 3228
Fragments arranged by size:
4486 3228 645 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2296 5632 1316
Size: 980 3336 4139
Fragments arranged by size:
4139 3336 980
XbaI T'CTAG_A
Cuts at: 5791 5791
Size: 8455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 4180 4414 4459 5161 5738 2167
Size: 2013 234 45 702 577 4884
Fragments arranged by size:
4884 2013 702 577 234 45
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8455
XmaI C'CCGG_G
Cuts at: 1870 2405 3599 5780 1870
Size: 535 1194 2181 4545
Fragments arranged by size:
4545 2181 1194 535
XmnI GAAnn'nnTTC
Cuts at: 3377 5204 6584 3377
Size: 1827 1380 5248
Fragments arranged by size:
5248 1827 1380
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6263 276
Size: 53 83 186 936 4729 2468
Fragments arranged by size:
4729 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BfrBI BsiWI BstZ17I FalI
FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SexAI SrfI SwaI