Restriction map for
pFN21AA0674
(Circular) (Six-base) MAPSORT of: pFN21AA0674.seq Check: 668 from: 1 to: 8362
pFN21AA0674 8362 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2491 4473 4951 2491
Size: 1982 478 5902
Fragments arranged by size:
5902 1982 478
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6172 278
Size: 53 83 186 936 4636 2468
Fragments arranged by size:
4636 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5705 1937
Size: 3768 4594
Acc65I G'GTAC_C
Cuts at: 5683 5683
Size: 8362
AclI AA'CG_TT
Cuts at: 1563 6489 6862 1563
Size: 4926 373 3063
Fragments arranged by size:
4926 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3224 3555 3848 4055 4511 4766 5286 6425
Size: 1592 331 293 207 456 255 520 1139
Cuts at: 6425 7809 8077 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1592 1384 1139 520 456 331 293
268 255 207
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8165
AflIII A'CryG_T
Cuts at: 1203 7261 8238 1203
Size: 6058 977 1327
Fragments arranged by size:
6058 1327 977
AgeI A'CCGG_T
Cuts at: 5399 7193 5399
Size: 1794 6568
AhdI GACnn_n'nnGTC
Cuts at: 7091 7091
Size: 8362
AleI CACnn'nnGTG
Cuts at: 1166 4070 1166
Size: 2904 5458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3665 1184
Size: 2481 5881
AlwNI CAG_nnn'CTG
Cuts at: 2099 4516 5045 6105 7677 2099
Size: 2417 529 1060 1572 2784
Fragments arranged by size:
2784 2417 1572 1060 529
ApaI G_GGCC'C
Cuts at: 5124 5124
Size: 8362
ApaLI G'TGCA_C
Cuts at: 6419 7575 6419
Size: 1156 7206
ApoI r'AATT_y
Cuts at: 1493 2114 2288 3558 4124 4293 5671 5897 6049
Size: 621 174 1270 566 169 1378 226 152
Cuts at: 6049 8158 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1378 1270 621 566 226 174
169 152
AseI AT'TA_AT
Cuts at: 160 1032 6916 160
Size: 872 5884 1606
Fragments arranged by size:
5884 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8362
AvaI C'yCGr_G
Cuts at: 1870 1943 4350 5309 5687 1870
Size: 73 2407 959 378 4545
Fragments arranged by size:
4545 2407 959 378 73
AvrII C'CTAG_G
Cuts at: 5149 5149
Size: 8362
BamHI G'GATC_C
Cuts at: 5692 6059 5692
Size: 367 7995
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2057 3280 3310 4948 5683
Size: 325 217 627 270 1223 30 1638 735
Cuts at: 5683 7138 618
Size: 1455 1842
Fragments arranged by size:
1842 1638 1455 1223 735 627 325 270
217 30
BanII G_rGCy'C
Cuts at: 1391 3306 4095 5124 5148 5681 1391
Size: 1915 789 1029 24 533 4072
Fragments arranged by size:
4072 1915 1029 789 533 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4870 5583 928
Size: 971 2971 713 3707
Fragments arranged by size:
3707 2971 971 713
BbvCI CC'TCA_GC
Cuts at: 3285 4888 5046 5358 3285
Size: 1603 158 312 6289
Fragments arranged by size:
6289 1603 312 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2648 2682 2817 2851 3437 3471 6553
Size: 34 711 34 135 34 586 34 3082
Cuts at: 6553 6587 1903
Size: 34 3678
Fragments arranged by size:
3678 3082 711 586 135 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5170 6254 7464 5170
Size: 1084 1210 6068
Fragments arranged by size:
6068 1210 1084
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8362
BfrBI ATG'CAT
Cuts at: 2249 2249
Size: 8362
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2066 3457 4550
Size: 107 122 71 631 605 394 1391 1093
Cuts at: 4550 6973 136
Size: 2423 1525
Fragments arranged by size:
2423 1525 1391 1093 631 605 394 122
107 71
BglII A'GATC_T
Cuts at: 4045 8357 4045
Size: 4312 4050
BlpI GC'TnA_GC
Cuts at: 4401 5211 5779 4401
Size: 810 568 6984
Fragments arranged by size:
6984 810 568
Bme1580I G_kGCm'C
Cuts at: 1790 4953 5124 6423 7579 1790
Size: 3163 171 1299 1156 2573
Fragments arranged by size:
3163 2573 1299 1156 171
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8362
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3309 4408 6093
Size: 416 53 83 399 24 1892 1099 1685
Cuts at: 6093 7051 442
Size: 958 1753
Fragments arranged by size:
1892 1753 1685 1099 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8362
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5071 5474 5071
Size: 403 7959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2766 3290 4199 4532 4736 7022 1239
Size: 1527 524 909 333 204 2286 2579
Fragments arranged by size:
2579 2286 1527 909 524 333 204
Bpu10I CC'TnA_GC
Cuts at: 3285 4888 5046 5142 5358 3285
Size: 1603 158 96 216 6289
Fragments arranged by size:
6289 1603 216 158 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2808 4148 4242 4893 5357 6487 7352 7650 2808
Size: 1340 94 651 464 1130 865 298 3520
Fragments arranged by size:
3520 1340 1130 865 651 464 298 94
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3964 7025 882
Size: 617 2465 3061 2219
Fragments arranged by size:
3061 2465 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6153 493
Size: 711 4949 2702
Fragments arranged by size:
4949 2702 711
BsaBI GATnn'nnATC
Cuts at: 8356 8356
Size: 8362
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5599 6169 6551 7186
Size: 53 83 186 936 4066 570 382 635
Cuts at: 7186 275
Size: 1451
Fragments arranged by size:
4066 1451 936 635 570 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4146 5399 6795 7193 7467 7614 8025 4146
Size: 1253 1396 398 274 147 411 4483
Fragments arranged by size:
4483 1396 1253 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8137
Fragments arranged by size:
8137 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3947 4196 4292 4295 4980 5195 5327 5579
Size: 2761 249 96 3 685 215 132 252
Cuts at: 5579 5582 1186
Size: 3 3966
Fragments arranged by size:
3966 2761 685 252 249 215 132 96
3 3
BseYI C'CCAG_C
Cuts at: 2644 2724 2740 3441 3469 5465 5478 5656 7565
Size: 80 16 701 28 1996 13 178 1909
Cuts at: 7565 8061 2644
Size: 496 2945
Fragments arranged by size:
2945 1996 1909 701 496 178 80 28
16 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4094 1163
Size: 573 2358 5431
Fragments arranged by size:
5431 2358 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5812 6573 7601 1540
Size: 275 186 3811 761 1028 2301
Fragments arranged by size:
3811 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2216 2550 4095 4464 6423 6508 7579 2216
Size: 334 1545 369 1959 85 1071 2999
Fragments arranged by size:
2999 1959 1545 1071 369 334 85
BsmI GAATG_Cn'
Cuts at: 2742 5873 5966 2742
Size: 3131 93 5138
Fragments arranged by size:
5138 3131 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2940 2940
Size: 8362
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2550 3306 4095 4464 4953 5124
Size: 399 426 334 756 789 369 489 171
Cuts at: 5124 5148 5681 6423 6508 7579 1391
Size: 24 533 742 85 1071 2174
Fragments arranged by size:
2174 1071 789 756 742 533 489 426
399 369 334 171 85 24
BspEI T'CCGG_A
Cuts at: 8025 8025
Size: 8362
BspHI T'CATG_A
Cuts at: 3528 3603 4674 4755 6251 7981 3528
Size: 75 1071 81 1496 1730 3909
Fragments arranged by size:
3909 1730 1496 1071 81 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2491 3386 3946 4473 4951 5423 5717 844
Size: 1647 895 560 527 478 472 294 3489
Fragments arranged by size:
3489 1647 895 560 527 478 472 294
BsrBI CCG'CTC
Cuts at: 1394 1823 2052 3478 4855 6249 1394
Size: 429 229 1426 1377 1394 3507
Fragments arranged by size:
3507 1426 1394 1377 429 229
BsrDI GCAATG_nn'
Cuts at: 65 1248 2508 6857 7031 65
Size: 1183 1260 4349 174 1396
Fragments arranged by size:
4349 1396 1260 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2618 3244 5399 7006 7193 1700
Size: 110 808 626 2155 1607 187 2869
Fragments arranged by size:
2869 2155 1607 808 626 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8362
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8362
BssSI C'ACGA_G
Cuts at: 6422 7434 6422
Size: 1012 7350
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2246 3317 794
Size: 1452 1071 5839
Fragments arranged by size:
5839 1452 1071
BstBI TT'CG_AA
Cuts at: 3562 7166 3562
Size: 3604 4758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8362
BstXI CCAn_nnnn'nTGG
Cuts at: 2227 4625 5203 2227
Size: 2398 578 5386
Fragments arranged by size:
5386 2398 578
BstYI r'GATC_y
Cuts at: 1972 3744 4045 5692 6059 6444 6461 7913 7999
Size: 1772 301 1647 367 385 17 1452 86
Cuts at: 7999 8011 8357 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1772 1647 1452 385 367 346 301
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4992 5332 5562 4992
Size: 340 230 7792
Fragments arranged by size:
7792 340 230
BtgI C'CryG_G
Cuts at: 513 1065 2104 4944 5163 513
Size: 552 1039 2840 219 3712
Fragments arranged by size:
3712 2840 1039 552 219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7623
Fragments arranged by size:
7623 683 56
BtsI GCAGTG_nn'
Cuts at: 2550 2664 3948 4831 5878 6672 6692 2550
Size: 114 1284 883 1047 794 20 4220
Fragments arranged by size:
4220 1284 1047 883 794 114 20
ClaI AT'CG_AT
Cuts at: 1554 2254 1554
Size: 700 7662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8327
DraI TTT'AAA
Cuts at: 5521 5666 5521
Size: 145 8217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4354 7369 809
Size: 507 3038 3015 1802
Fragments arranged by size:
3038 3015 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2101 3945 4856
Size: 54 1425 50 275 18 271 1844 911
Cuts at: 4856 5809 6698 8327 8
Size: 953 889 1629 43
Fragments arranged by size:
1844 1629 1425 953 911 889 275 271
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5809 1537
Size: 275 3997 4090
Fragments arranged by size:
4090 3997 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2536 3027 3243 3612 3842 4785 4809 4845
Size: 1183 491 216 369 230 943 24 36
Cuts at: 4845 5499 6292 1353
Size: 654 793 3423
Fragments arranged by size:
3423 1183 943 793 654 491 369 230
216 36 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4438 6949 7323 7469 387
Size: 1064 138 2849 2511 374 146 1280
Fragments arranged by size:
2849 2511 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4093 4093
Size: 8362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2766 3224 3290 3555 3848 4055 4199
Size: 393 1134 458 66 265 293 207 144
Cuts at: 4199 4511 4532 4736 4766 5286 6425 7022 7809
Size: 312 21 204 30 520 1139 597 787
Cuts at: 7809 8077 1239
Size: 268 1524
Fragments arranged by size:
1524 1139 1134 787 597 520 458 393
312 293 268 265 207 204 144 66
30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2148 4181 2148
Size: 2033 6329
EcoO109I rG'GnC_Cy
Cuts at: 2557 4996 5120 5121 5153 5320 5336 2557
Size: 2439 124 1 32 167 16 5583
Fragments arranged by size:
5583 2439 167 124 32 16 1
EcoRI G'AATT_C
Cuts at: 5671 6049 5671
Size: 378 7984
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3764 4234 3764
Size: 470 7892
FspI TGC'GCA
Cuts at: 3954 4489 6868 3954
Size: 535 2379 5448
Fragments arranged by size:
5448 2379 535
FspAI rTGC'GCAy
Cuts at: 4489 4489
Size: 8362
HaeII r_GCGC'y
Cuts at: 1678 7509 1678
Size: 5831 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2876 2908 2945
Size: 32 135 32 455 32 817 32 37
Cuts at: 2945 2977 3338 3370 4199 4231 4370 4402 4762
Size: 32 361 32 829 32 139 32 360
Cuts at: 4762 4794 5210 5242 7003 7035 7077 7109 1373
Size: 32 416 32 1761 32 42 32 2626
Fragments arranged by size:
2626 1761 829 817 455 416 361 360
139 135 42 37 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2354 5706 5952 669
Size: 988 281 416 3352 246 3079
Fragments arranged by size:
3352 3079 988 416 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8362
HpaI GTT'AAC
Cuts at: 5952 5952
Size: 8362
KpnI G_GTAC'C
Cuts at: 5687 5687
Size: 8362
MfeI C'AATT_G
Cuts at: 5961 5961
Size: 8362
MluI A'CGCG_T
Cuts at: 8238 8238
Size: 8362
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2451 2572 7476 7660 1305
Size: 1146 121 4904 184 2007
Fragments arranged by size:
4904 2007 1146 184 121
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2103 10
Size: 54 1425 343 271 6269
Fragments arranged by size:
6269 1425 343 271 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2109 3692 4070 6320 6679 6838 518
Size: 648 943 1583 378 2250 359 159 2042
Fragments arranged by size:
2250 2042 1583 943 648 378 359 159
MspA1I CmG'CkG
Cuts at: 1606 2096 3151 3359 3445 3944 3973 4021 4373
Size: 490 1055 208 86 499 29 48 352
Cuts at: 4373 4384 4786 5778 6455 7603 7848 1606
Size: 11 402 992 677 1148 245 2120
Fragments arranged by size:
2120 1148 1055 992 677 499 490 402
352 245 208 86 48 29 11
NaeI GCC'GGC
Cuts at: 3246 3246
Size: 8362
NcoI C'CATG_G
Cuts at: 513 1065 2104 4944 5163 513
Size: 552 1039 2840 219 3712
Fragments arranged by size:
3712 2840 1039 552 219
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8362
NgoMIV G'CCGG_C
Cuts at: 3244 3244
Size: 8362
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8362
NotI GC'GGCC_GC
Cuts at: 5809 5809
Size: 8362
NsiI A_TGCA'T
Cuts at: 2251 2251
Size: 8362
NspI r_CATG'y
Cuts at: 1132 1229 3667 3905 5720 7265 1132
Size: 97 2438 238 1815 1545 2229
Fragments arranged by size:
2438 2229 1815 1545 238 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8362
PciI A'CATG_T
Cuts at: 7261 7261
Size: 8362
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8362
PmeI GTTT'AAAC
Cuts at: 5666 5666
Size: 8362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6431 6463 7944 7976 6431
Size: 32 1481 32 6817
Fragments arranged by size:
6817 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2557 5320 2557
Size: 2763 5599
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8362
PsiI TTA'TAA
Cuts at: 5932 5932
Size: 8362
PspOMI G'GGCC_C
Cuts at: 5120 5120
Size: 8362
PspXI vC'TCGA_Gb
Cuts at: 4350 4350
Size: 8362
PstI C_TGCA'G
Cuts at: 830 2302 2929 3450 3972 4014 4378 4470 5714
Size: 1472 627 521 522 42 364 92 1244
Cuts at: 5714 830
Size: 3478
Fragments arranged by size:
3478 1472 1244 627 522 521 364 92
42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8362
PvuII CAG'CTG
Cuts at: 2096 3151 3359 3445 3973 4021 4373 4384 4786
Size: 1055 208 86 528 48 352 11 402
Cuts at: 4786 2096
Size: 5672
Fragments arranged by size:
5672 1055 528 402 352 208 86 48
11
SacI G_AGCT'C
Cuts at: 4095 4095
Size: 8362
SalI G'TCGA_C
Cuts at: 1936 5704 1936
Size: 3768 4594
SanDI GG'GwC_CC
Cuts at: 5320 5320
Size: 8362
SapI GCTCTTCn'nnn_
Cuts at: 3027 4845 3027
Size: 1818 6544
SbfI CC_TGCA'GG
Cuts at: 5714 5714
Size: 8362
ScaI AGT'ACT
Cuts at: 6610 8322 6610
Size: 1712 6650
SexAI A'CCwGG_T
Cuts at: 4680 4680
Size: 8362
SfcI C'TryA_G
Cuts at: 826 1045 2298 2419 2583 2925 3446 3968 4010
Size: 219 1253 121 164 342 521 522 42
Cuts at: 4010 4374 4406 4466 5710 6845 7526 7717 826
Size: 364 32 60 1244 1135 681 191 1471
Fragments arranged by size:
1471 1253 1244 1135 681 522 521 364
342 219 191 164 121 60 42 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8362
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8362
SmaI CCC'GGG
Cuts at: 1872 5689 1872
Size: 3817 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2823 4163 4257 4350 4908 5372
Size: 197 926 880 1340 94 93 558 464
Cuts at: 5372 6466 7367 7629 820
Size: 1094 901 262 1553
Fragments arranged by size:
1553 1340 1094 926 901 880 558 464
262 197 94 93
SnaBI TAC'GTA
Cuts at: 493 6153 493
Size: 5660 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8362
SphI G_CATG'C
Cuts at: 1132 3905 5720 1132
Size: 2773 1815 3774
Fragments arranged by size:
3774 2773 1815
SspI AAT'ATT
Cuts at: 5 52 6286 5
Size: 47 6234 2081
Fragments arranged by size:
6234 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8362
StyI C'CwwG_G
Cuts at: 513 1065 2104 3499 3921 4218 4452 4944 5149
Size: 552 1039 1395 422 297 234 492 205
Cuts at: 5149 5163 5801 513
Size: 14 638 3074
Fragments arranged by size:
3074 1395 1039 638 552 492 422 297
234 205 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3001 6389 6406 6559 6744 7189 325
Size: 1255 1421 3388 17 153 185 445 1498
Fragments arranged by size:
3388 1498 1421 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 4393 6608
Size: 274 80 33 51 1443 147 2269 2215
Cuts at: 6608 8320 96
Size: 1712 138
Fragments arranged by size:
2269 2215 1712 1443 274 147 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5159 5191 8322 8354 5159
Size: 32 3131 32 5167
Fragments arranged by size:
5167 3131 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3220 1316
Size: 1904 6458
XbaI T'CTAG_A
Cuts at: 5698 5698
Size: 8362
XhoI C'TCGA_G
Cuts at: 1943 4350 1943
Size: 2407 5955
XmaI C'CCGG_G
Cuts at: 1870 5687 1870
Size: 3817 4545
XmnI GAAnn'nnTTC
Cuts at: 4867 6491 4867
Size: 1624 6738
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6170 276
Size: 53 83 186 936 4636 2468
Fragments arranged by size:
4636 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BsiWI BstZ17I DraIII
EcoRV FseI KasI NarI NruI PacI PfoI PmlI
PsrI RsrII SacII SfiI SfoI SrfI SwaI XcmI