Restriction map for
pFN21AA0672
(Circular) (Six-base) MAPSORT of: pFN21AA0672.seq Check: 4028 from: 1 to: 7159
pFN21AA0672 7159 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4969 278
Size: 53 83 186 936 3433 2468
Fragments arranged by size:
3433 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4316 4367 4502 1937
Size: 2379 51 135 4594
Fragments arranged by size:
4594 2379 135 51
Acc65I G'GTAC_C
Cuts at: 4480 4480
Size: 7159
AclI AA'CG_TT
Cuts at: 1563 5286 5659 1563
Size: 3723 373 3063
Fragments arranged by size:
3723 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2078 2475 2921 3068 5222 6606 6874 1632
Size: 446 397 446 147 2154 1384 268 1917
Fragments arranged by size:
2154 1917 1384 446 446 397 268 147
AfeI AGC'GCT
Cuts at: 2198 2198
Size: 7159
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6962
AflIII A'CryG_T
Cuts at: 1203 3388 6058 7035 1203
Size: 2185 2670 977 1327
Fragments arranged by size:
2670 2185 1327 977
AgeI A'CCGG_T
Cuts at: 5990 5990
Size: 7159
AhdI GACnn_n'nnGTC
Cuts at: 5888 5888
Size: 7159
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7159
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7159
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3300 3332 3338 3370 3300
Size: 32 6 32 7089
Fragments arranged by size:
7089 32 32 6
AlwNI CAG_nnn'CTG
Cuts at: 2189 2236 2329 3697 3800 4415 4902 6474 2189
Size: 47 93 1368 103 615 487 1572 2874
Fragments arranged by size:
2874 1572 1368 615 487 103 93 47
ApaI G_GGCC'C
Cuts at: 3756 3756
Size: 7159
ApaLI G'TGCA_C
Cuts at: 5216 6372 5216
Size: 1156 6003
ApoI r'AATT_y
Cuts at: 1493 3152 4468 4694 4846 6955 1493
Size: 1659 1316 226 152 2109 1697
Fragments arranged by size:
2109 1697 1659 1316 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5713 160
Size: 872 4681 1606
Fragments arranged by size:
4681 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7159
AvaI C'yCGr_G
Cuts at: 1870 1943 3458 3755 4108 4361 4484 1870
Size: 73 1515 297 353 253 123 4545
Fragments arranged by size:
4545 1515 353 297 253 123 73
BamHI G'GATC_C
Cuts at: 3045 4489 4856 3045
Size: 1444 367 5348
Fragments arranged by size:
5348 1444 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2522 3450 3884 3958 4480
Size: 325 217 627 735 928 434 74 522
Cuts at: 4480 5935 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 928 735 627 522 434 325
217 74
BanII G_rGCy'C
Cuts at: 1391 2373 3756 3865 3877 4362 4478 1391
Size: 982 1383 109 12 485 116 4072
Fragments arranged by size:
4072 1383 982 485 116 109 12
BbeI G_GCGC'C
Cuts at: 3454 3888 3454
Size: 434 6725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2091 2481 928
Size: 971 192 390 5606
Fragments arranged by size:
5606 971 390 192
BbvCI CC'TCA_GC
Cuts at: 3479 4227 3479
Size: 748 6411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2003 2037 3644 3678 4438 4472 5350
Size: 34 66 34 1607 34 760 34 878
Cuts at: 5350 5384 1903
Size: 34 3678
Fragments arranged by size:
3678 1607 878 760 66 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5051 6261 5051
Size: 1210 5949
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7159
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3659 5770 136
Size: 107 122 71 631 605 1987 2111 1525
Fragments arranged by size:
2111 1987 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4321 7154 4321
Size: 2833 4326
BlpI GC'TnA_GC
Cuts at: 3876 4576 3876
Size: 700 6459
Bme1580I G_kGCm'C
Cuts at: 1790 3756 3836 5220 6376 1790
Size: 1966 80 1384 1156 2573
Fragments arranged by size:
2573 1966 1384 1156 80
BmgBI CAC'GTC
Cuts at: 1318 2362 1318
Size: 1044 6115
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3195 3583 4025
Size: 416 53 83 399 24 1778 388 442
Cuts at: 4025 4890 5848 442
Size: 865 958 1753
Fragments arranged by size:
1778 1753 958 865 442 416 399 388
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7159
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4104 4230 4104
Size: 126 7033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2795 3527 3728 3777 3808 3891 4409
Size: 893 663 732 201 49 31 83 518
Cuts at: 4409 5819 1239
Size: 1410 2579
Fragments arranged by size:
2579 1410 893 732 663 518 201 83
49 31
Bpu10I CC'TnA_GC
Cuts at: 2322 3479 4194 4227 2322
Size: 1157 715 33 5254
Fragments arranged by size:
5254 1157 715 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2864 5284 6149 6447 2864
Size: 2420 865 298 3576
Fragments arranged by size:
3576 2420 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4201 5822 882
Size: 617 2702 1621 2219
Fragments arranged by size:
2702 2219 1621 617
BsaAI yAC'GTr
Cuts at: 493 1204 3391 4950 493
Size: 711 2187 1559 2702
Fragments arranged by size:
2702 2187 1559 711
BsaBI GATnn'nnATC
Cuts at: 7153 7153
Size: 7159
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3451 3885 4966 5348
Size: 53 83 186 936 1918 434 1081 382
Cuts at: 5348 5983 275
Size: 635 1451
Fragments arranged by size:
1918 1451 1081 936 635 434 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3936 5592 5990 6264 6411 6822 3936
Size: 1656 398 274 147 411 4273
Fragments arranged by size:
4273 1656 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2183 2213 3216 3246 3338
Size: 30 165 30 780 30 1003 30 92
Cuts at: 3338 3368 4424 4454 1178
Size: 30 1056 30 3883
Fragments arranged by size:
3883 1056 1003 780 165 92 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2792 2876 3583 4125 4215 4448 4451 1186
Size: 1606 84 707 542 90 233 3 3894
Fragments arranged by size:
3894 1606 707 542 233 90 84 3
BseYI C'CCAG_C
Cuts at: 3893 3899 4055 4123 6362 6858 3893
Size: 6 156 68 2239 496 4194
Fragments arranged by size:
4194 2239 496 156 68 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2963 3786 3864 1163
Size: 573 1227 823 78 4458
Fragments arranged by size:
4458 1227 823 573 78
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2800 3600 3604 4609 5370 6398
Size: 275 186 799 800 4 1005 761 1028
Cuts at: 6398 1540
Size: 2301
Fragments arranged by size:
2301 1028 1005 800 799 761 275 186
4
BsiHKAI G_wGCw'C
Cuts at: 2784 3877 4362 4395 4451 5220 5305 6376 2784
Size: 1093 485 33 56 769 85 1071 3567
Fragments arranged by size:
3567 1093 1071 769 485 85 56 33
BsmI GAATG_Cn'
Cuts at: 3103 4670 4763 3103
Size: 1567 93 5499
Fragments arranged by size:
5499 1567 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2297 2352 2297
Size: 55 7104
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2373 2784 3756 3836 3865 3877 4362
Size: 399 583 411 972 80 29 12 485
Cuts at: 4362 4395 4451 4478 5220 5305 6376 1391
Size: 33 56 27 742 85 1071 2174
Fragments arranged by size:
2174 1071 972 742 583 485 411 399
85 80 56 33 29 27 12
BspEI T'CCGG_A
Cuts at: 3936 6822 3936
Size: 2886 4273
BspHI T'CATG_A
Cuts at: 5048 6778 5048
Size: 1730 5429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4514 844
Size: 3670 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5046 1394
Size: 429 3223 3507
Fragments arranged by size:
3507 3223 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5654 5828 65
Size: 1183 4406 174 1396
Fragments arranged by size:
4406 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3447 3471 3599 5803 5990 1700
Size: 110 1637 24 128 2204 187 2869
Fragments arranged by size:
2869 2204 1637 187 128 110 24
BsrGI T'GTAC_A
Cuts at: 96 2607 96
Size: 2511 4648
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7159
BssSI C'ACGA_G
Cuts at: 5219 6231 5219
Size: 1012 6147
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7159
BstBI TT'CG_AA
Cuts at: 5963 5963
Size: 7159
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7159
BstXI CCAn_nnnn'nTGG
Cuts at: 2572 3208 4016 2572
Size: 636 808 5715
Fragments arranged by size:
5715 808 636
BstYI r'GATC_y
Cuts at: 1972 3045 4321 4489 4856 5241 5258 6710 6796
Size: 1073 1276 168 367 385 17 1452 86
Cuts at: 6796 6808 7154 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1276 1073 385 367 346 168
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2540 3530 4089 2540
Size: 990 559 5610
Fragments arranged by size:
5610 990 559
BtgI C'CryG_G
Cuts at: 513 1065 3489 3708 4269 513
Size: 552 2424 219 561 3403
Fragments arranged by size:
3403 2424 561 552 219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6420
Fragments arranged by size:
6420 683 56
BtsI GCAGTG_nn'
Cuts at: 4675 5469 5489 4675
Size: 794 20 6345
Fragments arranged by size:
6345 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7159
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3558 3593 529
Size: 35 2994 35 4095
Fragments arranged by size:
4095 2994 35 35
DraI TTT'AAA
Cuts at: 4463 4463
Size: 7159
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2485 3280 6166 809
Size: 507 1169 795 2886 1802
Fragments arranged by size:
2886 1802 1169 795 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2619 2797
Size: 54 1425 50 275 18 207 582 178
Cuts at: 2797 3239 3597 3985 4018 4606 5495 7124 8
Size: 442 358 388 33 588 889 1629 43
Fragments arranged by size:
1629 1425 889 588 582 442 388 358
275 207 178 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2797 3597 4606 1537
Size: 275 985 800 1009 4090
Fragments arranged by size:
4090 1009 985 800 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2550 2876 3580 5089 1353
Size: 1197 326 704 1509 3423
Fragments arranged by size:
3423 1509 1197 704 326
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2402 4364 5746 6120 6266 387
Size: 1064 138 813 1962 1382 374 146 1280
Fragments arranged by size:
1962 1382 1280 1064 813 374 146 138
EcoICRI GAG'CTC
Cuts at: 3875 4360 3875
Size: 485 6674
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2132 2475 2795 2921 3068 3527
Size: 393 446 54 343 320 126 147 459
Cuts at: 3527 3728 3777 3808 3891 4409 5222 5819 6606
Size: 201 49 31 83 518 813 597 787
Cuts at: 6606 6874 1239
Size: 268 1524
Fragments arranged by size:
1524 813 787 597 518 459 446 393
343 320 268 201 147 126 83 54
49 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2093 2093
Size: 7159
EcoO109I rG'GnC_Cy
Cuts at: 3224 3976 4276 3224
Size: 752 300 6107
Fragments arranged by size:
6107 752 300
EcoRI G'AATT_C
Cuts at: 4468 4846 4468
Size: 378 6781
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2108 2776 3997 2108
Size: 668 1221 5270
Fragments arranged by size:
5270 1221 668
FspI TGC'GCA
Cuts at: 5665 5665
Size: 7159
HaeII r_GCGC'y
Cuts at: 1678 2200 3454 3888 6306 1678
Size: 522 1254 434 2418 2531
Fragments arranged by size:
2531 2418 1254 522 434
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 2579 2611 3216
Size: 32 135 32 611 32 364 32 605
Cuts at: 3216 3248 4280 4312 4328 4349 4360 4381 4424
Size: 32 1032 32 16 21 11 21 43
Cuts at: 4424 4456 5800 5832 5874 5906 1373
Size: 32 1344 32 42 32 2626
Fragments arranged by size:
2626 1344 1032 611 605 364 135 43
42 32 32 32 32 32 32 32
32 32 21 21 16 11
HincII GTy'rAC
Cuts at: 669 1657 1938 4368 4503 4749 669
Size: 988 281 2430 135 246 3079
Fragments arranged by size:
3079 2430 988 281 246 135
HindIII A'AGCT_T
Cuts at: 748 3080 748
Size: 2332 4827
HpaI GTT'AAC
Cuts at: 4749 4749
Size: 7159
KasI G'GCGC_C
Cuts at: 3450 3884 3450
Size: 434 6725
KpnI G_GTAC'C
Cuts at: 4484 4484
Size: 7159
MfeI C'AATT_G
Cuts at: 2970 3213 4758 2970
Size: 243 1545 5371
Fragments arranged by size:
5371 1545 243
MluI A'CGCG_T
Cuts at: 7035 7035
Size: 7159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3584 6273 6457 1305
Size: 2279 2689 184 2007
Fragments arranged by size:
2689 2279 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 2621 3241 3987 4020
Size: 54 1425 343 207 582 620 746 33
Cuts at: 4020 10
Size: 3149
Fragments arranged by size:
3149 1425 746 620 582 343 207 54
33
MslI CAynn'nnrTG
Cuts at: 518 1166 3551 5117 5476 5635 518
Size: 648 2385 1566 359 159 2042
Fragments arranged by size:
2385 2042 1566 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2600 2773 2796 3682 3710 4016 4024
Size: 429 565 173 23 886 28 306 8
Cuts at: 4024 4127 4575 5252 6400 6645 1606
Size: 103 448 677 1148 245 2120
Fragments arranged by size:
2120 1148 886 677 565 448 429 306
245 173 103 28 23 8
NaeI GCC'GGC
Cuts at: 3473 3473
Size: 7159
NarI GG'CG_CC
Cuts at: 3451 3885 3451
Size: 434 6725
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6607
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7159
NgoMIV G'CCGG_C
Cuts at: 3471 3471
Size: 7159
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7159
NotI GC'GGCC_GC
Cuts at: 4606 4606
Size: 7159
NspI r_CATG'y
Cuts at: 1132 1229 2029 2163 3326 4297 4315 4399 4517
Size: 97 800 134 1163 971 18 84 118
Cuts at: 4517 6062 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1163 971 800 134 118 97
84 18
PasI CC'CwG_GG
Cuts at: 1348 3341 4301 1348
Size: 1993 960 4206
Fragments arranged by size:
4206 1993 960
PciI A'CATG_T
Cuts at: 6058 6058
Size: 7159
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2050 3547 1108
Size: 942 1497 4720
Fragments arranged by size:
4720 1497 942
PfoI T'CCnGG_A
Cuts at: 3063 3063
Size: 7159
PmeI GTTT'AAAC
Cuts at: 4463 4463
Size: 7159
PmlI CAC'GTG
Cuts at: 3391 3391
Size: 7159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 5228 5260 6741 6773 3338
Size: 32 1858 32 1481 32 3724
Fragments arranged by size:
3724 1858 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3224 3976 3224
Size: 752 6407
PshAI GACnn'nnGTC
Cuts at: 1613 3921 1613
Size: 2308 4851
PsiI TTA'TAA
Cuts at: 4729 4729
Size: 7159
PspOMI G'GGCC_C
Cuts at: 3752 3752
Size: 7159
PstI C_TGCA'G
Cuts at: 830 2169 3916 4132 4511 830
Size: 1339 1747 216 379 3478
Fragments arranged by size:
3478 1747 1339 379 216
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7159
PvuII CAG'CTG
Cuts at: 2035 2600 4024 4127 2035
Size: 565 1424 103 5067
Fragments arranged by size:
5067 1424 565 103
SacI G_AGCT'C
Cuts at: 3877 4362 3877
Size: 485 6674
SacII CC_GC'GG
Cuts at: 3711 3711
Size: 7159
SalI G'TCGA_C
Cuts at: 1936 4366 4501 1936
Size: 2430 135 4594
Fragments arranged by size:
4594 2430 135
SbfI CC_TGCA'GG
Cuts at: 4511 4511
Size: 7159
ScaI AGT'ACT
Cuts at: 2951 5407 7119 2951
Size: 2456 1712 2991
Fragments arranged by size:
2991 2456 1712
SexAI A'CCwGG_T
Cuts at: 2465 2465
Size: 7159
SfcI C'TryA_G
Cuts at: 826 1045 2165 3409 3912 4128 4317 4507 5642
Size: 219 1120 1244 503 216 189 190 1135
Cuts at: 5642 6323 6514 826
Size: 681 191 1471
Fragments arranged by size:
1471 1244 1135 1120 681 503 219 216
191 190 189
SfoI GGC'GCC
Cuts at: 3452 3886 3452
Size: 434 6725
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7159
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7159
SmaI CCC'GGG
Cuts at: 1872 4486 1872
Size: 2614 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2843 5263 6164 6426 820
Size: 197 926 900 2420 901 262 1553
Fragments arranged by size:
2420 1553 926 901 900 262 197
SnaBI TAC'GTA
Cuts at: 493 4950 493
Size: 4457 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7159
SphI G_CATG'C
Cuts at: 1132 2029 3326 4517 1132
Size: 897 1297 1191 3774
Fragments arranged by size:
3774 1297 1191 897
SspI AAT'ATT
Cuts at: 5 52 2407 5083 5
Size: 47 2355 2676 2081
Fragments arranged by size:
2676 2355 2081 47
StuI AGG'CCT
Cuts at: 1517 2741 1517
Size: 1224 5935
StyI C'CwwG_G
Cuts at: 513 1065 2526 3117 3972 4101 4598 513
Size: 552 1461 591 855 129 497 3074
Fragments arranged by size:
3074 1461 855 591 552 497 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3539 3565 3985 5186 5203 5356 5541
Size: 1255 1959 26 420 1201 17 153 185
Cuts at: 5541 5986 325
Size: 445 1498
Fragments arranged by size:
1959 1498 1255 1201 445 420 185 153
26 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2607 2949 3385
Size: 274 80 33 51 1443 630 342 436
Cuts at: 3385 5405 7117 96
Size: 2020 1712 138
Fragments arranged by size:
2020 1712 1443 630 436 342 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2672 2704 3088 3120 3960 3992 7119 7151 2672
Size: 32 384 32 840 32 3127 32 2680
Fragments arranged by size:
3127 2680 840 384 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7159
XbaI T'CTAG_A
Cuts at: 4495 4495
Size: 7159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2047 2440 2047
Size: 393 6766
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7159
XmaI C'CCGG_G
Cuts at: 1870 4484 1870
Size: 2614 4545
XmnI GAAnn'nnTTC
Cuts at: 2015 2088 5288 2015
Size: 73 3200 3886
Fragments arranged by size:
3886 3200 73
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4967 276
Size: 53 83 186 936 3433 2468
Fragments arranged by size:
3433 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI
RsrII SanDI SapI SfiI SrfI SwaI