Restriction map for
pFN21AA0660
(Circular) (Six-base) MAPSORT of: pFN21AA0660.seq Check: 8444 from: 1 to: 6151
pFN21AA0660 6151 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3494 1937
Size: 1557 4594
Acc65I G'GTAC_C
Cuts at: 3472 3472
Size: 6151
AclI AA'CG_TT
Cuts at: 1563 3072 4278 4651 1563
Size: 1509 1206 373 3063
Fragments arranged by size:
3063 1509 1206 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2197 2373 2643 2730 4214 5598 5866 1632
Size: 565 176 270 87 1484 1384 268 1917
Fragments arranged by size:
1917 1484 1384 565 270 268 176 87
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5954
AflIII A'CryG_T
Cuts at: 1203 5050 6027 1203
Size: 3847 977 1327
Fragments arranged by size:
3847 1327 977
AgeI A'CCGG_T
Cuts at: 4982 4982
Size: 6151
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2529 1184
Size: 1345 4806
AlwNI CAG_nnn'CTG
Cuts at: 2609 2702 3894 5466 2609
Size: 93 1192 1572 3294
Fragments arranged by size:
3294 1572 1192 93
ApaLI G'TGCA_C
Cuts at: 4208 5364 4208
Size: 1156 4995
ApoI r'AATT_y
Cuts at: 1493 2113 2232 2372 2796 3050 3121 3460 3686
Size: 620 119 140 424 254 71 339 226
Cuts at: 3686 3838 5947 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 620 424 339 254 226 152
140 119 71
AseI AT'TA_AT
Cuts at: 160 1032 4705 160
Size: 872 3673 1606
Fragments arranged by size:
3673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 3476 1870
Size: 73 1533 4545
Fragments arranged by size:
4545 1533 73
BamHI G'GATC_C
Cuts at: 3481 3848 3481
Size: 367 5784
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3472 4927 618
Size: 325 217 627 1685 1455 1842
Fragments arranged by size:
1842 1685 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3470 1391
Size: 2079 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4342 4376 1903
Size: 34 2405 34 3678
Fragments arranged by size:
3678 2405 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4043 5253 4043
Size: 1210 4941
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6151
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4762 136
Size: 107 122 71 631 605 3090 1525
Fragments arranged by size:
3090 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6146 6146
Size: 6151
BlpI GC'TnA_GC
Cuts at: 3568 3568
Size: 6151
Bme1580I G_kGCm'C
Cuts at: 1790 4212 5368 1790
Size: 2422 1156 2573
Fragments arranged by size:
2573 2422 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6151
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2016 3882 4840
Size: 416 53 83 399 24 599 1866 958
Cuts at: 4840 442
Size: 1753
Fragments arranged by size:
1866 1753 958 599 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4811 1239
Size: 3572 2579
Bpu10I CC'TnA_GC
Cuts at: 2598 2598
Size: 6151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2281 4276 5141 5439 2281
Size: 1995 865 298 2993
Fragments arranged by size:
2993 1995 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4814 882
Size: 617 3315 2219
Fragments arranged by size:
3315 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3317 3942 493
Size: 711 2113 625 2702
Fragments arranged by size:
2702 2113 711 625
BsaBI GATnn'nnATC
Cuts at: 2994 6145 2994
Size: 3151 3000
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3958 4340 4975 275
Size: 53 83 186 936 2425 382 635 1451
Fragments arranged by size:
2425 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2083 4584 4982 5256 5403 5814 2083
Size: 2501 398 274 147 411 2420
Fragments arranged by size:
2501 2420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2262 2292 2671 2674 2701
Size: 30 165 30 859 30 379 3 27
Cuts at: 2701 2704 1178
Size: 3 4625
Fragments arranged by size:
4625 859 379 165 30 30 30 27
3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2576 2660 2676 2679 2684 2952 3357 1186
Size: 1390 84 16 3 5 268 405 3980
Fragments arranged by size:
3980 1390 405 268 84 16 5 3
BseYI C'CCAG_C
Cuts at: 5354 5850 5354
Size: 496 5655
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5578
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3601 4362 5390 1540
Size: 275 186 1600 761 1028 2301
Fragments arranged by size:
2301 1600 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4212 4297 5368 4212
Size: 85 1071 4995
Fragments arranged by size:
4995 1071 85
BsmI GAATG_Cn'
Cuts at: 3662 3755 3662
Size: 93 6058
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3470 4212 4297 5368 1391
Size: 399 1680 742 85 1071 2174
Fragments arranged by size:
2174 1680 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2083 5814 2083
Size: 3731 2420
BspHI T'CATG_A
Cuts at: 2584 3057 4040 5770 2584
Size: 473 983 1730 2965
Fragments arranged by size:
2965 1730 983 473
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2772 3506 844
Size: 1928 734 3489
Fragments arranged by size:
3489 1928 734
BsrBI CCG'CTC
Cuts at: 1394 1823 4038 1394
Size: 429 2215 3507
Fragments arranged by size:
3507 2215 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3298 4646 4820 65
Size: 1183 2050 1348 174 1396
Fragments arranged by size:
2050 1396 1348 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4795 4982 1700
Size: 110 2985 187 2869
Fragments arranged by size:
2985 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6151
BssSI C'ACGA_G
Cuts at: 2871 4211 5223 2871
Size: 1340 1012 3799
Fragments arranged by size:
3799 1340 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6151
BstBI TT'CG_AA
Cuts at: 4955 4955
Size: 6151
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6151
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6151
BstYI r'GATC_y
Cuts at: 1972 2357 3481 3848 4233 4250 5702 5788 5800
Size: 385 1124 367 385 17 1452 86 12
Cuts at: 5800 6146 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1124 385 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4660
Fragments arranged by size:
4660 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5412
Fragments arranged by size:
5412 683 56
BtsI GCAGTG_nn'
Cuts at: 3667 4461 4481 3667
Size: 794 20 5337
Fragments arranged by size:
5337 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6116
DraI TTT'AAA
Cuts at: 3210 3455 3210
Size: 245 5906
DraIII CAC_nnn'GTG
Cuts at: 2545 2873 2545
Size: 328 5823
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5158 809
Size: 507 3842 1802
Fragments arranged by size:
3842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2173 3598 4487
Size: 54 1425 50 275 18 343 1425 889
Cuts at: 4487 6116 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1425 889 343 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3598 1537
Size: 275 1786 4090
Fragments arranged by size:
4090 1786 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2460 2619 3213 3352 3400 4081 1353
Size: 1107 159 594 139 48 681 3423
Fragments arranged by size:
3423 1107 681 594 159 139 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4738 5112 5258 387
Size: 1064 138 3149 374 146 1280
Fragments arranged by size:
3149 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2197 2373 2643 2730 4214 4811 5598
Size: 393 565 176 270 87 1484 597 787
Cuts at: 5598 5866 1239
Size: 268 1524
Fragments arranged by size:
1524 1484 787 597 565 393 270 268
176 87
EcoNI CCTnn'n_nnAGG
Cuts at: 2916 2916
Size: 6151
EcoO109I rG'GnC_Cy
Cuts at: 3302 3362 3302
Size: 60 6091
EcoRI G'AATT_C
Cuts at: 2113 2796 3050 3460 3838 2113
Size: 683 254 410 378 4426
Fragments arranged by size:
4426 683 410 378 254
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3070 3070
Size: 6151
FspI TGC'GCA
Cuts at: 4657 4657
Size: 6151
HaeII r_GCGC'y
Cuts at: 1678 5298 1678
Size: 3620 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2262 2294 2432 2464 2591
Size: 32 135 32 690 32 138 32 127
Cuts at: 2591 2623 2660 2692 3331 3363 4792 4824 4866
Size: 32 37 32 639 32 1429 32 42
Cuts at: 4866 4898 1373
Size: 32 2626
Fragments arranged by size:
2626 1429 690 639 138 135 127 42
37 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3495 3741 669
Size: 988 281 1557 246 3079
Fragments arranged by size:
3079 1557 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3113 748
Size: 2365 3786
HpaI GTT'AAC
Cuts at: 3741 3741
Size: 6151
KpnI G_GTAC'C
Cuts at: 3476 3476
Size: 6151
MfeI C'AATT_G
Cuts at: 3750 3750
Size: 6151
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5265 5449 1305
Size: 3960 184 2007
Fragments arranged by size:
3960 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2175 10
Size: 54 1425 343 343 3986
Fragments arranged by size:
3986 1425 343 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2244 4109 4468 4627 518
Size: 648 843 235 1865 359 159 2042
Fragments arranged by size:
2042 1865 843 648 359 235 159
MspA1I CmG'CkG
Cuts at: 1606 2029 3567 4244 5392 5637 1606
Size: 423 1538 677 1148 245 2120
Fragments arranged by size:
2120 1538 1148 677 423 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4660
Fragments arranged by size:
4660 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6151
NotI GC'GGCC_GC
Cuts at: 3598 3598
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 3509 5054 1132
Size: 97 2280 1545 2229
Fragments arranged by size:
2280 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6151
PciI A'CATG_T
Cuts at: 5050 5050
Size: 6151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2287 3125 3377 1108
Size: 1179 838 252 3882
Fragments arranged by size:
3882 1179 838 252
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PmlI CAC'GTG
Cuts at: 3317 3317
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4220 4252 5733 5765 4220
Size: 32 1481 32 4606
Fragments arranged by size:
4606 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3302 3362 3302
Size: 60 6091
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6151
PsiI TTA'TAA
Cuts at: 3721 3721
Size: 6151
PstI C_TGCA'G
Cuts at: 830 3503 830
Size: 2673 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
SalI G'TCGA_C
Cuts at: 1936 3493 1936
Size: 1557 4594
SapI GCTCTTCn'nnn_
Cuts at: 2619 2619
Size: 6151
SbfI CC_TGCA'GG
Cuts at: 3503 3503
Size: 6151
ScaI AGT'ACT
Cuts at: 4399 6111 4399
Size: 1712 4439
SexAI A'CCwGG_T
Cuts at: 2902 2902
Size: 6151
SfcI C'TryA_G
Cuts at: 826 1045 3499 4634 5315 5506 826
Size: 219 2454 1135 681 191 1471
Fragments arranged by size:
2454 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6151
SmaI CCC'GGG
Cuts at: 1872 3478 1872
Size: 1606 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2260 4255 5156 5418 820
Size: 197 926 317 1995 901 262 1553
Fragments arranged by size:
1995 1553 926 901 317 262 197
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6151
SphI G_CATG'C
Cuts at: 1132 3509 1132
Size: 2377 3774
SspI AAT'ATT
Cuts at: 5 52 2060 2090 4075 5
Size: 47 2008 30 1985 2081
Fragments arranged by size:
2081 2008 1985 47 30
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6151
StyI C'CwwG_G
Cuts at: 513 1065 2004 3096 3371 3590 513
Size: 552 939 1092 275 219 3074
Fragments arranged by size:
3074 1092 939 552 275 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3317 4178 4195 4348 4533 4978 325
Size: 1255 1737 861 17 153 185 445 1498
Fragments arranged by size:
1737 1498 1255 861 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4397 6109 96
Size: 274 80 33 51 1443 2420 1712 138
Fragments arranged by size:
2420 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3060 3092 6111 6143 3060
Size: 32 3019 32 3068
Fragments arranged by size:
3068 3019 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3444 1316
Size: 2128 4023
XbaI T'CTAG_A
Cuts at: 3487 3487
Size: 6151
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6151
XmaI C'CCGG_G
Cuts at: 1870 3476 1870
Size: 1606 4545
XmnI GAAnn'nnTTC
Cuts at: 3156 4280 3156
Size: 1124 5027
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI