Restriction map for
pFN21AA0656
(Circular) (Six-base) MAPSORT of: pFN21AA0656.seq Check: 5534 from: 1 to: 7426
pFN21AA0656 7426 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3127 4663 3127
Size: 1536 5890
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5236 278
Size: 53 83 186 936 3700 2468
Fragments arranged by size:
3700 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2366 4769 1937
Size: 429 2403 4594
Fragments arranged by size:
4594 2403 429
Acc65I G'GTAC_C
Cuts at: 4225 4319 4747 4225
Size: 94 428 6904
Fragments arranged by size:
6904 428 94
AclI AA'CG_TT
Cuts at: 1563 5553 5926 1563
Size: 3990 373 3063
Fragments arranged by size:
3990 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3213 3330 3474 3741 3894 4328 5489 6873
Size: 1581 117 144 267 153 434 1161 1384
Cuts at: 6873 7141 1632
Size: 268 1917
Fragments arranged by size:
1917 1581 1384 1161 434 268 267 153
144 117
AfeI AGC'GCT
Cuts at: 3952 3952
Size: 7426
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7229
AflIII A'CryG_T
Cuts at: 1203 6325 7302 1203
Size: 5122 977 1327
Fragments arranged by size:
5122 1327 977
AgeI A'CCGG_T
Cuts at: 2983 6257 2983
Size: 3274 4152
AhdI GACnn_n'nnGTC
Cuts at: 6155 6155
Size: 7426
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7426
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7426
AlwNI CAG_nnn'CTG
Cuts at: 2063 2217 3272 3470 3599 3749 5169 6741 2063
Size: 154 1055 198 129 150 1420 1572 2748
Fragments arranged by size:
2748 1572 1420 1055 198 154 150 129
ApaI G_GGCC'C
Cuts at: 2114 4495 2114
Size: 2381 5045
ApaLI G'TGCA_C
Cuts at: 4437 5483 6639 4437
Size: 1046 1156 5224
Fragments arranged by size:
5224 1156 1046
ApoI r'AATT_y
Cuts at: 1493 2543 2709 2750 4311 4735 4961 5113 7222
Size: 1050 166 41 1561 424 226 152 2109
Cuts at: 7222 1493
Size: 1697
Fragments arranged by size:
2109 1697 1561 1050 424 226 166 152
41
AseI AT'TA_AT
Cuts at: 160 1032 5980 160
Size: 872 4948 1606
Fragments arranged by size:
4948 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7426
AvaI C'yCGr_G
Cuts at: 1870 1943 4751 1870
Size: 73 2808 4545
Fragments arranged by size:
4545 2808 73
BamHI G'GATC_C
Cuts at: 2345 4756 5123 2345
Size: 2411 367 4648
Fragments arranged by size:
4648 2411 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4039 4192 4225 4319 4747
Size: 325 217 627 2252 153 33 94 428
Cuts at: 4747 6202 618
Size: 1455 1842
Fragments arranged by size:
2252 1842 1455 627 428 325 217 153
94 33
BanII G_rGCy'C
Cuts at: 1391 2114 4495 4745 1391
Size: 723 2381 250 4072
Fragments arranged by size:
4072 2381 723 250
BbeI G_GCGC'C
Cuts at: 4043 4043
Size: 7426
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2910 928
Size: 971 1011 5444
Fragments arranged by size:
5444 1011 971
BbvCI CC'TCA_GC
Cuts at: 3265 4563 3265
Size: 1298 6128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5617 5651 1903
Size: 34 3680 34 3678
Fragments arranged by size:
3680 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5318 6528 5318
Size: 1210 6216
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7426
BfrBI ATG'CAT
Cuts at: 3422 3857 3938 3422
Size: 435 81 6910
Fragments arranged by size:
6910 435 81
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6037 136
Size: 107 122 71 631 605 4365 1525
Fragments arranged by size:
4365 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2611 4339 7421 2611
Size: 1728 3082 2616
Fragments arranged by size:
3082 2616 1728
BlpI GC'TnA_GC
Cuts at: 4843 4843
Size: 7426
Bme1580I G_kGCm'C
Cuts at: 1790 2114 4441 4495 5487 6643 1790
Size: 324 2327 54 992 1156 2573
Fragments arranged by size:
2573 2327 1156 992 324 54
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7426
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3014 4227 5157
Size: 416 53 83 399 24 1597 1213 930
Cuts at: 5157 6115 442
Size: 958 1753
Fragments arranged by size:
1753 1597 1213 958 930 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3543 1056
Size: 2487 4939
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2907 3044 3099 3144 4038 4047 4377 4389
Size: 1668 137 55 45 894 9 330 12
Cuts at: 4389 4533 4665 6086 1239
Size: 144 132 1421 2579
Fragments arranged by size:
2579 1668 1421 894 330 144 137 132
55 45 12 9
Bpu10I CC'TnA_GC
Cuts at: 3265 3477 3738 4563 3265
Size: 212 261 825 6128
Fragments arranged by size:
6128 825 261 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2741 5551 6416 6714 2741
Size: 2810 865 298 3453
Fragments arranged by size:
3453 2810 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2150 3138 3503 6089 882
Size: 617 651 988 365 2586 2219
Fragments arranged by size:
2586 2219 988 651 617 365
BsaAI yAC'GTr
Cuts at: 493 1204 5217 493
Size: 711 4013 2702
Fragments arranged by size:
4013 2702 711
BsaBI GATnn'nnATC
Cuts at: 2553 7420 2553
Size: 4867 2559
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4040 5233 5615 6250
Size: 53 83 186 936 2507 1193 382 635
Cuts at: 6250 275
Size: 1451
Fragments arranged by size:
2507 1451 1193 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2983 3553 5859 6257 6531 6678 7089 2983
Size: 570 2306 398 274 147 411 3320
Fragments arranged by size:
3320 2306 570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2467 2497 2797 2827 3535
Size: 30 165 30 1064 30 300 30 708
Cuts at: 3535 3565 3658 3661 3688 3691 1178
Size: 30 93 3 27 3 4913
Fragments arranged by size:
4913 1064 708 300 165 93 30 30
30 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3141 3666 3778 1186
Size: 1955 525 112 4834
Fragments arranged by size:
4834 1955 525 112
BseYI C'CCAG_C
Cuts at: 2217 2816 4199 4538 6629 7125 2217
Size: 599 1383 339 2091 496 2518
Fragments arranged by size:
2518 2091 1383 599 496 339
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3156 3456 3483 4194 1163
Size: 573 1420 300 27 711 4395
Fragments arranged by size:
4395 1420 711 573 300 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4876 5637 6665 1540
Size: 275 186 2875 761 1028 2301
Fragments arranged by size:
2875 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2899 4441 5487 5572 6643 2899
Size: 1542 1046 85 1071 3682
Fragments arranged by size:
3682 1542 1071 1046 85
BsmI GAATG_Cn'
Cuts at: 4357 4533 4937 5030 4357
Size: 176 404 93 6753
Fragments arranged by size:
6753 404 176 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2825 2946 2825
Size: 121 7305
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2899 4441 4495 4745 5487 5572
Size: 399 324 785 1542 54 250 742 85
Cuts at: 5572 6643 1391
Size: 1071 2174
Fragments arranged by size:
2174 1542 1071 785 742 399 324 250
85 54
BspEI T'CCGG_A
Cuts at: 7089 7089
Size: 7426
BspHI T'CATG_A
Cuts at: 2098 5315 7045 2098
Size: 3217 1730 2479
Fragments arranged by size:
3217 2479 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3127 3976 4086 4663 4781 844
Size: 2283 849 110 577 118 3489
Fragments arranged by size:
3489 2283 849 577 118 110
BsrBI CCG'CTC
Cuts at: 1394 1823 2042 2078 2200 5313 1394
Size: 429 219 36 122 3113 3507
Fragments arranged by size:
3507 3113 429 219 122 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 3501 4248 5921 6095 65
Size: 1183 2253 747 1673 174 1396
Fragments arranged by size:
2253 1673 1396 1183 747 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2983 4570 6070 6257 1700
Size: 110 1173 1587 1500 187 2869
Fragments arranged by size:
2869 1587 1500 1173 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7426
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7426
BssSI C'ACGA_G
Cuts at: 2739 5486 6498 2739
Size: 2747 1012 3667
Fragments arranged by size:
3667 2747 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3419 3470 3599 3884 794
Size: 2625 51 129 285 4336
Fragments arranged by size:
4336 2625 285 129 51
BstBI TT'CG_AA
Cuts at: 6230 6230
Size: 7426
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7426
BstXI CCAn_nnnn'nTGG
Cuts at: 4413 4413
Size: 7426
BstYI r'GATC_y
Cuts at: 1972 2345 2611 2888 3208 3316 4339 4702 4756
Size: 373 266 277 320 108 1023 363 54
Cuts at: 4756 5123 5508 5525 6977 7063 7075 7421 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1023 385 373 367 363 346
320 277 266 108 86 54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2350 4188 513
Size: 552 1285 1838 3751
Fragments arranged by size:
3751 1838 1285 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6687
Fragments arranged by size:
6687 683 56
BtsI GCAGTG_nn'
Cuts at: 2534 3132 3303 3669 3831 3882 4942 5736 5756
Size: 598 171 366 162 51 1060 794 20
Cuts at: 5756 2534
Size: 4204
Fragments arranged by size:
4204 1060 794 598 366 171 162 51
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7426
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7391
DraI TTT'AAA
Cuts at: 4730 4730
Size: 7426
DraIII CAC_nnn'GTG
Cuts at: 4436 4436
Size: 7426
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2743 6433 809
Size: 507 1427 3690 1802
Fragments arranged by size:
3690 1802 1427 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 4873 5762
Size: 54 1425 50 275 18 351 2692 889
Cuts at: 5762 7391 8
Size: 1629 43
Fragments arranged by size:
2692 1629 1425 889 351 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4873 1537
Size: 275 3061 4090
Fragments arranged by size:
4090 3061 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5356 1353
Size: 4003 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6013 6387 6533 387
Size: 1064 138 4424 374 146 1280
Fragments arranged by size:
4424 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2907 3044 3099 3144 3213 3330 3474
Size: 393 1275 137 55 45 69 117 144
Cuts at: 3474 3741 3894 4038 4047 4328 4377 4389 4533
Size: 267 153 144 9 281 49 12 144
Cuts at: 4533 4665 5489 6086 6873 7141 1239
Size: 132 824 597 787 268 1524
Fragments arranged by size:
1524 1275 824 787 597 393 281 268
267 153 144 144 144 137 132 117
69 55 49 45 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2374 2807 3123 4446 4515 2374
Size: 433 316 1323 69 5285
Fragments arranged by size:
5285 1323 433 316 69
EcoO109I rG'GnC_Cy
Cuts at: 2111 3355 3804 4491 4492 4599 4690 2111
Size: 1244 449 687 1 107 91 4847
Fragments arranged by size:
4847 1244 687 449 107 91 1
EcoRI G'AATT_C
Cuts at: 4735 5113 4735
Size: 378 7048
EcoRV GAT'ATC
Cuts at: 3691 3691
Size: 7426
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2934 3969 4458 4738 2934
Size: 1035 489 280 5622
Fragments arranged by size:
5622 1035 489 280
FspI TGC'GCA
Cuts at: 5932 5932
Size: 7426
HaeII r_GCGC'y
Cuts at: 1678 2383 3737 3954 4043 6573 1678
Size: 705 1354 217 89 2530 2531
Fragments arranged by size:
2531 2530 1354 705 217 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2236 2268 2467 2499 3059
Size: 32 135 32 664 32 199 32 560
Cuts at: 3059 3091 3193 3225 3770 3802 3830 3862 4051
Size: 32 102 32 545 32 28 32 189
Cuts at: 4051 4083 6067 6099 6141 6173 1373
Size: 32 1984 32 42 32 2626
Fragments arranged by size:
2626 1984 664 560 545 199 189 135
102 42 32 32 32 32 32 32
32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 4371 4770 5016 669
Size: 988 281 2433 399 246 3079
Fragments arranged by size:
3079 2433 988 399 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7426
HpaI GTT'AAC
Cuts at: 5016 5016
Size: 7426
KasI G'GCGC_C
Cuts at: 4039 4039
Size: 7426
KpnI G_GTAC'C
Cuts at: 4229 4323 4751 4229
Size: 94 428 6904
Fragments arranged by size:
6904 428 94
MfeI C'AATT_G
Cuts at: 5025 5025
Size: 7426
MluI A'CGCG_T
Cuts at: 7302 7302
Size: 7426
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4483 4487 6540 6724 1305
Size: 3178 4 2053 184 2007
Fragments arranged by size:
3178 2053 2007 184 4
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5604
Fragments arranged by size:
5604 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2271 2355 2559 4553 5384 5743 5902
Size: 648 1105 84 204 1994 831 359 159
Cuts at: 5902 518
Size: 2042
Fragments arranged by size:
2042 1994 1105 831 648 359 204 159
84
MspA1I CmG'CkG
Cuts at: 1606 3596 3629 3656 4199 4619 4842 5519 6667
Size: 1990 33 27 543 420 223 677 1148
Cuts at: 6667 6912 1606
Size: 245 2120
Fragments arranged by size:
2120 1990 1148 677 543 420 245 223
33 27
NarI GG'CG_CC
Cuts at: 4040 4040
Size: 7426
NcoI C'CATG_G
Cuts at: 513 1065 2350 4188 513
Size: 552 1285 1838 3751
Fragments arranged by size:
3751 1838 1285 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7426
NheI G'CTAG_C
Cuts at: 1052 3539 1052
Size: 2487 4939
NotI GC'GGCC_GC
Cuts at: 4873 4873
Size: 7426
NsiI A_TGCA'T
Cuts at: 3424 3859 3940 3424
Size: 435 81 6910
Fragments arranged by size:
6910 435 81
NspI r_CATG'y
Cuts at: 1132 1229 3426 4552 4784 6329 1132
Size: 97 2197 1126 232 1545 2229
Fragments arranged by size:
2229 2197 1545 1126 232 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7426
PciI A'CATG_T
Cuts at: 6325 6325
Size: 7426
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3122 3138 4502 1108
Size: 2014 16 1364 4032
Fragments arranged by size:
4032 2014 1364 16
PfoI T'CCnGG_A
Cuts at: 3051 3051
Size: 7426
PmeI GTTT'AAAC
Cuts at: 4730 4730
Size: 7426
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5495 5527 7008 7040 5495
Size: 32 1481 32 5881
Fragments arranged by size:
5881 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4690 4690
Size: 7426
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7426
PsiI TTA'TAA
Cuts at: 4996 4996
Size: 7426
PspOMI G'GGCC_C
Cuts at: 2110 4491 2110
Size: 2381 5045
PstI C_TGCA'G
Cuts at: 830 3364 3733 4778 830
Size: 2534 369 1045 3478
Fragments arranged by size:
3478 2534 1045 369
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7426
PvuII CAG'CTG
Cuts at: 3596 4199 3596
Size: 603 6823
SalI G'TCGA_C
Cuts at: 1936 4768 1936
Size: 2832 4594
SbfI CC_TGCA'GG
Cuts at: 4778 4778
Size: 7426
ScaI AGT'ACT
Cuts at: 5674 7386 5674
Size: 1712 5714
SexAI A'CCwGG_T
Cuts at: 4412 4412
Size: 7426
SfcI C'TryA_G
Cuts at: 826 1045 2067 2383 2516 3360 3402 3522 3534
Size: 219 1022 316 133 844 42 120 12
Cuts at: 3534 3729 4082 4774 5909 6590 6781 826
Size: 195 353 692 1135 681 191 1471
Fragments arranged by size:
1471 1135 1022 844 692 681 353 316
219 195 191 133 120 42 12
SfoI GGC'GCC
Cuts at: 4041 4041
Size: 7426
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7426
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7426
SmaI CCC'GGG
Cuts at: 1872 4753 1872
Size: 2881 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2756 5530 6431 6693 820
Size: 197 926 813 2774 901 262 1553
Fragments arranged by size:
2774 1553 926 901 813 262 197
SnaBI TAC'GTA
Cuts at: 493 5217 493
Size: 4724 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7426
SphI G_CATG'C
Cuts at: 1132 4552 4784 1132
Size: 3420 232 3774
Fragments arranged by size:
3774 3420 232
SspI AAT'ATT
Cuts at: 5 52 2170 2291 5350 5
Size: 47 2118 121 3059 2081
Fragments arranged by size:
3059 2118 2081 121 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7426
StyI C'CwwG_G
Cuts at: 513 1065 2350 4188 4261 4865 513
Size: 552 1285 1838 73 604 3074
Fragments arranged by size:
3074 1838 1285 604 552 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2209 4246 5453 5470 5623 5808 6253
Size: 1255 629 2037 1207 17 153 185 445
Cuts at: 6253 325
Size: 1498
Fragments arranged by size:
2037 1498 1255 1207 629 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2046 3773 5672
Size: 274 80 33 51 1443 69 1727 1899
Cuts at: 5672 7384 96
Size: 1712 138
Fragments arranged by size:
1899 1727 1712 1443 274 138 80 69
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2797 2829 3535 3567 3658 3661 3690 3693 7386
Size: 32 706 32 91 3 29 3 3693
Cuts at: 7386 7418 2797
Size: 32 2805
Fragments arranged by size:
3693 2805 706 91 32 32 32 29
3 3
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7426
XbaI T'CTAG_A
Cuts at: 2300 2704 3685 4762 2300
Size: 404 981 1077 4964
Fragments arranged by size:
4964 1077 981 404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2824 4240 2824
Size: 1416 6010
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7426
XmaI C'CCGG_G
Cuts at: 1870 4751 1870
Size: 2881 4545
XmnI GAAnn'nnTTC
Cuts at: 5555 5555
Size: 7426
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5234 276
Size: 53 83 186 936 3700 2468
Fragments arranged by size:
3700 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BplI BsiWI BstZ17I Bsu36I
EcoICRI FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SrfI SwaI