Restriction map for
pFN21AA0633
(Circular) (Six-base) MAPSORT of: pFN21AA0633.seq Check: 2817 from: 1 to: 8488
pFN21AA0633 8488 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3142 5501 3142
Size: 2359 6129
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2199 3825 6298 278
Size: 53 83 186 936 663 1626 2473 2468
Fragments arranged by size:
2473 2468 1626 936 663 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4889 5831 1937
Size: 2952 942 4594
Fragments arranged by size:
4594 2952 942
Acc65I G'GTAC_C
Cuts at: 5809 5809
Size: 8488
AclI AA'CG_TT
Cuts at: 1563 6615 6988 1563
Size: 5052 373 3063
Fragments arranged by size:
5052 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2270 2316 2442 4296 6551 7935 8203 1632
Size: 638 46 126 1854 2255 1384 268 1917
Fragments arranged by size:
2255 1917 1854 1384 638 268 126 46
AflII C'TTAA_G
Cuts at: 820 1017 4106 820
Size: 197 3089 5202
Fragments arranged by size:
5202 3089 197
AflIII A'CryG_T
Cuts at: 1203 2670 7387 8364 1203
Size: 1467 4717 977 1327
Fragments arranged by size:
4717 1467 1327 977
AgeI A'CCGG_T
Cuts at: 4012 7319 4012
Size: 3307 5181
AhdI GACnn_n'nnGTC
Cuts at: 7217 7217
Size: 8488
AleI CACnn'nnGTG
Cuts at: 1166 4388 1166
Size: 3222 5266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4479 1184
Size: 3295 5193
AlwNI CAG_nnn'CTG
Cuts at: 3129 3157 5033 5756 6231 7803 3129
Size: 28 1876 723 475 1572 3814
Fragments arranged by size:
3814 1876 1572 723 475 28
ApaI G_GGCC'C
Cuts at: 2137 2137
Size: 8488
ApaLI G'TGCA_C
Cuts at: 2102 6545 7701 2102
Size: 4443 1156 2889
Fragments arranged by size:
4443 2889 1156
ApoI r'AATT_y
Cuts at: 1493 2322 2744 4003 5285 5797 6023 6175 8284
Size: 829 422 1259 1282 512 226 152 2109
Cuts at: 8284 1493
Size: 1697
Fragments arranged by size:
2109 1697 1282 1259 829 512 422 226
152
AseI AT'TA_AT
Cuts at: 160 1032 3455 7042 160
Size: 872 2423 3587 1606
Fragments arranged by size:
3587 2423 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8488
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 2651 5813 1870
Size: 73 195 513 3162 4545
Fragments arranged by size:
4545 3162 513 195 73
AvrII C'CTAG_G
Cuts at: 4489 4853 4489
Size: 364 8124
BamHI G'GATC_C
Cuts at: 5818 6185 5818
Size: 367 8121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2452 3742 3886 4054 4068
Size: 325 217 627 665 1290 144 168 14
Cuts at: 4068 4540 4702 4787 5489 5690 5773 5809 7264
Size: 472 162 85 702 201 83 36 1455
Cuts at: 7264 618
Size: 1842
Fragments arranged by size:
1842 1455 1290 702 665 627 472 325
217 201 168 162 144 85 83 36
14
BanII G_rGCy'C
Cuts at: 1391 2137 2522 2652 2667 2911 3148 3371 4364
Size: 746 385 130 15 244 237 223 993
Cuts at: 4364 4639 4849 5040 5535 5573 5807 1391
Size: 275 210 191 495 38 234 4072
Fragments arranged by size:
4072 993 746 495 385 275 244 237
234 223 210 191 130 38 15
BbeI G_GCGC'C
Cuts at: 3746 3890 4706 3746
Size: 144 816 7528
Fragments arranged by size:
7528 816 144
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2322 3321 3453 3504 3522 4254 4938
Size: 971 423 999 132 51 18 732 684
Cuts at: 4938 5594 5598 928
Size: 656 4 3818
Fragments arranged by size:
3818 999 971 732 684 656 423 132
51 18 4
BbvCI CC'TCA_GC
Cuts at: 2177 5696 2177
Size: 3519 4969
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3596 3630 5438 5472 6679 6713 1903
Size: 34 1659 34 1808 34 1207 34 3678
Fragments arranged by size:
3678 1808 1659 1207 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6380 7590 6380
Size: 1210 7278
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 2218 7099
Size: 107 122 71 631 605 355 191 4881
Cuts at: 7099 136
Size: 1525
Fragments arranged by size:
4881 1525 631 605 355 191 122 107
71
BglII A'GATC_T
Cuts at: 8483 8483
Size: 8488
BlpI GC'TnA_GC
Cuts at: 3756 5026 5905 3756
Size: 1270 879 6339
Fragments arranged by size:
6339 1270 879
Bme1580I G_kGCm'C
Cuts at: 1790 2106 2137 4395 4792 5484 5693 5778 6549
Size: 316 31 2258 397 692 209 85 771
Cuts at: 6549 7705 1790
Size: 1156 2573
Fragments arranged by size:
2573 2258 1156 771 692 397 316 209
85 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2543 3617 4786
Size: 416 53 83 399 24 1126 1074 1169
Cuts at: 4786 5208 6219 7177 442
Size: 422 1011 958 1753
Fragments arranged by size:
1753 1169 1126 1074 1011 958 422 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8488
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5041 5041
Size: 8488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2243 2519 2645 2904 3483 4336 5145 5519
Size: 1004 276 126 259 579 853 809 374
Cuts at: 5519 5659 7148 1239
Size: 140 1489 2579
Fragments arranged by size:
2579 1489 1004 853 809 579 374 276
259 140 126
Bpu10I CC'TnA_GC
Cuts at: 2177 5565 5696 2177
Size: 3388 131 4969
Fragments arranged by size:
4969 3388 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3969 4559 6613 7478 7776 3969
Size: 590 2054 865 298 4681
Fragments arranged by size:
4681 2054 865 590 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3167 5299 7151 882
Size: 617 1668 2132 1852 2219
Fragments arranged by size:
2219 2132 1852 1668 617
BsaAI yAC'GTr
Cuts at: 493 1204 2587 4534 6279 493
Size: 711 1383 1947 1745 2702
Fragments arranged by size:
2702 1947 1745 1383 711
BsaBI GATnn'nnATC
Cuts at: 3870 8482 3870
Size: 4612 3876
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2196 3743 3822 3887
Size: 53 83 186 936 663 1547 79 65
Cuts at: 3887 4703 5733 6295 6677 7312 275
Size: 816 1030 562 382 635 1451
Fragments arranged by size:
1547 1451 1030 936 816 663 635 562
382 186 83 79 65 53
BsaWI w'CCGG_w
Cuts at: 4012 4187 6921 7319 7593 7740 8151 4012
Size: 175 2734 398 274 147 411 4349
Fragments arranged by size:
4349 2734 411 398 274 175 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3164 3194 3524 3554 4907
Size: 30 165 30 1761 30 330 30 1353
Cuts at: 4907 4937 1178
Size: 30 4729
Fragments arranged by size:
4729 1761 1353 330 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2245 2585 2633 2904 2907 3092 3185 4800
Size: 1059 340 48 271 3 185 93 1615
Cuts at: 4800 5543 1186
Size: 743 4131
Fragments arranged by size:
4131 1615 1059 743 340 271 185 93
48 3
BseYI C'CCAG_C
Cuts at: 2839 2857 3695 4210 4452 5261 7691 8187 2839
Size: 18 838 515 242 809 2430 496 3140
Fragments arranged by size:
3140 2430 838 809 515 496 242 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3788 4701 5315 5442 5643 1163
Size: 573 2052 913 614 127 201 4008
Fragments arranged by size:
4008 2052 913 614 573 201 127
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2043 3216 5938 6699 7727 1540
Size: 275 186 42 1173 2722 761 1028 2301
Fragments arranged by size:
2722 2301 1173 1028 761 275 186 42
BsiHKAI G_wGCw'C
Cuts at: 2068 2106 2241 2586 2652 2667 3095 3762 4364
Size: 38 135 345 66 15 428 667 602
Cuts at: 4364 5535 6549 6634 7705 2068
Size: 1171 1014 85 1071 2851
Fragments arranged by size:
2851 1171 1071 1014 667 602 428 345
135 85 66 38 15
BsmI GAATG_Cn'
Cuts at: 5999 6092 5999
Size: 93 8395
BsmBI CGTCTCn'nnnn_
Cuts at: 3259 3259
Size: 8488
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2106 2137 2241 2522 2586 2652
Size: 399 278 38 31 104 281 64 66
Cuts at: 2652 2667 2911 3095 3148 3371 3762 4364 4395
Size: 15 244 184 53 223 391 602 31
Cuts at: 4395 4639 4792 4849 5040 5484 5535 5573 5693
Size: 244 153 57 191 444 51 38 120
Cuts at: 5693 5778 5807 6549 6634 7705 1391
Size: 85 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 602 444 399 391 281
278 244 244 223 191 184 153 120
104 85 85 66 64 57 53 51
38 38 31 31 29 15
BspEI T'CCGG_A
Cuts at: 8151 8151
Size: 8488
BspHI T'CATG_A
Cuts at: 6377 8107 6377
Size: 1730 6758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2496 3142 3805 4912 5501 5843 844
Size: 1652 646 663 1107 589 342 3489
Fragments arranged by size:
3489 1652 1107 663 646 589 342
BsrBI CCG'CTC
Cuts at: 1394 1823 5742 6375 1394
Size: 429 3919 633 3507
Fragments arranged by size:
3919 3507 633 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2607 2722 4617 6983 7157 65
Size: 1183 1359 115 1895 2366 174 1396
Fragments arranged by size:
2366 1895 1396 1359 1183 174 115
BsrFI r'CCGG_y
Cuts at: 1700 1810 4012 4238 7132 7319 1700
Size: 110 2202 226 2894 187 2869
Fragments arranged by size:
2894 2869 2202 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8488
BssHII G'CGCG_C
Cuts at: 1925 2017 5128 1925
Size: 92 3111 5285
Fragments arranged by size:
5285 3111 92
BssSI C'ACGA_G
Cuts at: 2061 5783 6548 7560 2061
Size: 3722 765 1012 2989
Fragments arranged by size:
3722 2989 1012 765
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3129 5519 5561 794
Size: 2335 2390 42 3721
Fragments arranged by size:
3721 2390 2335 42
BstBI TT'CG_AA
Cuts at: 3911 7292 3911
Size: 3381 5107
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8488
BstXI CCAn_nnnn'nTGG
Cuts at: 2191 4065 4525 2191
Size: 1874 460 6154
Fragments arranged by size:
6154 1874 460
BstYI r'GATC_y
Cuts at: 1972 5818 6185 6570 6587 8039 8125 8137 8483
Size: 3846 367 385 17 1452 86 12 346
Cuts at: 8483 1972
Size: 1977
Fragments arranged by size:
3846 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2448 2523 2597 5501 2448
Size: 75 74 2904 5435
Fragments arranged by size:
5435 2904 75 74
BtgI C'CryG_G
Cuts at: 513 1065 2004 2190 2202 3559 4518 4822 5140
Size: 552 939 186 12 1357 959 304 318
Cuts at: 5140 5476 513
Size: 336 3525
Fragments arranged by size:
3525 1357 959 939 552 336 318 304
186 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2270 4171 487
Size: 683 56 1044 1901 4804
Fragments arranged by size:
4804 1901 1044 683 56
BtsI GCAGTG_nn'
Cuts at: 3127 3328 3574 4845 5459 6004 6798 6818 3127
Size: 201 246 1271 614 545 794 20 4797
Fragments arranged by size:
4797 1271 794 614 545 246 201 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8453
DraI TTT'AAA
Cuts at: 5792 5792
Size: 8488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7495 809
Size: 507 6179 1802
Fragments arranged by size:
6179 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 5255 5935
Size: 54 1425 50 275 18 198 3227 680
Cuts at: 5935 6824 8453 8
Size: 889 1629 43
Fragments arranged by size:
3227 1629 1425 889 680 275 198 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5935 1537
Size: 275 4123 4090
Fragments arranged by size:
4123 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 2418 2680 3083 3359 6418 1353
Size: 869 196 262 403 276 3059 3423
Fragments arranged by size:
3423 3059 869 403 276 262 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3182 7075 7449 7595 387
Size: 1064 138 1593 3893 374 146 1280
Fragments arranged by size:
3893 1593 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2650 2665 4362 5533 2650
Size: 15 1697 1171 5605
Fragments arranged by size:
5605 1697 1171 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2270 2316 2442 2519 2645 2904
Size: 393 611 27 46 126 77 126 259
Cuts at: 2904 3483 4296 4336 5145 5519 5659 6551 7148
Size: 579 813 40 809 374 140 892 597
Cuts at: 7148 7935 8203 1239
Size: 787 268 1524
Fragments arranged by size:
1524 892 813 809 787 611 597 579
393 374 268 259 140 126 126 77
46 40 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2919 4493 5101 5163 5504 2919
Size: 1574 608 62 341 5903
Fragments arranged by size:
5903 1574 608 341 62
EcoO109I rG'GnC_Cy
Cuts at: 2133 2134 2379 3331 3356 4373 2133
Size: 1 245 952 25 1017 6248
Fragments arranged by size:
6248 1017 952 245 25 1
EcoRI G'AATT_C
Cuts at: 4003 5797 6175 4003
Size: 1794 378 6316
Fragments arranged by size:
6316 1794 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3388 5794 3388
Size: 2406 6082
FspI TGC'GCA
Cuts at: 6994 6994
Size: 8488
HaeII r_GCGC'y
Cuts at: 1678 2177 2905 3011 3746 3890 4706 7635 1678
Size: 499 728 106 735 144 816 2929 2531
Fragments arranged by size:
2929 2531 816 735 728 499 144 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2044 2076 2936 2968 3164
Size: 32 135 32 472 32 860 32 196
Cuts at: 3164 3196 3239 3271 3524 3556 4393 4425 4733
Size: 32 43 32 253 32 837 32 308
Cuts at: 4733 4765 7129 7161 7203 7235 1373
Size: 32 2364 32 42 32 2626
Fragments arranged by size:
2626 2364 860 837 472 308 253 196
135 43 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5832 6078 669
Size: 988 281 3894 246 3079
Fragments arranged by size:
3894 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8488
HpaI GTT'AAC
Cuts at: 6078 6078
Size: 8488
KasI G'GCGC_C
Cuts at: 3742 3886 4702 3742
Size: 144 816 7528
Fragments arranged by size:
7528 816 144
KpnI G_GTAC'C
Cuts at: 5813 5813
Size: 8488
MfeI C'AATT_G
Cuts at: 6087 6087
Size: 8488
MluI A'CGCG_T
Cuts at: 2670 8364 2670
Size: 5694 2794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2868 3183 3594 3805 7602 7786 1305
Size: 1563 315 411 211 3797 184 2007
Fragments arranged by size:
3797 2007 1563 411 315 211 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5257 10
Size: 54 1425 343 3425 3241
Fragments arranged by size:
3425 3241 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4388 4523 4653 4674 4774 6446 6805
Size: 648 3222 135 130 21 100 1672 359
Cuts at: 6805 6964 518
Size: 159 2042
Fragments arranged by size:
3222 2042 1672 648 359 159 135 130
100 21
MspA1I CmG'CkG
Cuts at: 1606 3192 5478 5487 5904 6581 7729 7974 1606
Size: 1586 2286 9 417 677 1148 245 2120
Fragments arranged by size:
2286 2120 1586 1148 677 417 245 9
NarI GG'CG_CC
Cuts at: 3743 3887 4703 3743
Size: 144 816 7528
Fragments arranged by size:
7528 816 144
NcoI C'CATG_G
Cuts at: 513 1065 2004 2190 3559 4518 513
Size: 552 939 186 1369 959 4483
Fragments arranged by size:
4483 1369 959 939 552 186
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8488
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8488
NotI GC'GGCC_GC
Cuts at: 5935 5935
Size: 8488
NspI r_CATG'y
Cuts at: 1132 1229 2102 5846 7391 1132
Size: 97 873 3744 1545 2229
Fragments arranged by size:
3744 2229 1545 873 97
PasI CC'CwG_GG
Cuts at: 1348 3112 3891 4088 1348
Size: 1764 779 197 5748
Fragments arranged by size:
5748 1764 779 197
PciI A'CATG_T
Cuts at: 7387 7387
Size: 8488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2078 4606 1108
Size: 970 2528 4990
Fragments arranged by size:
4990 2528 970
PfoI T'CCnGG_A
Cuts at: 2077 5161 2077
Size: 3084 5404
PmeI GTTT'AAAC
Cuts at: 5792 5792
Size: 8488
PmlI CAC'GTG
Cuts at: 2587 4534 2587
Size: 1947 6541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6557 6589 8070 8102 6557
Size: 32 1481 32 6943
Fragments arranged by size:
6943 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2379 3331 3356 4373 2379
Size: 952 25 1017 6494
Fragments arranged by size:
6494 1017 952 25
PshAI GACnn'nnGTC
Cuts at: 1613 3354 5598 1613
Size: 1741 2244 4503
Fragments arranged by size:
4503 2244 1741
PsiI TTA'TAA
Cuts at: 6058 6058
Size: 8488
PspOMI G'GGCC_C
Cuts at: 2133 2133
Size: 8488
PstI C_TGCA'G
Cuts at: 830 3134 3323 5102 5498 5840 830
Size: 2304 189 1779 396 342 3478
Fragments arranged by size:
3478 2304 1779 396 342 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8488
PvuII CAG'CTG
Cuts at: 3192 3192
Size: 8488
SacI G_AGCT'C
Cuts at: 2652 2667 4364 5535 2652
Size: 15 1697 1171 5605
Fragments arranged by size:
5605 1697 1171 15
SacII CC_GC'GG
Cuts at: 5479 5479
Size: 8488
SalI G'TCGA_C
Cuts at: 1936 5830 1936
Size: 3894 4594
SanDI GG'GwC_CC
Cuts at: 2379 3356 4373 2379
Size: 977 1017 6494
Fragments arranged by size:
6494 1017 977
SapI GCTCTTCn'nnn_
Cuts at: 3083 3359 3083
Size: 276 8212
SbfI CC_TGCA'GG
Cuts at: 5840 5840
Size: 8488
ScaI AGT'ACT
Cuts at: 6736 8448 6736
Size: 1712 6776
SfcI C'TryA_G
Cuts at: 826 1045 3130 3319 4099 5098 5226 5494 5836
Size: 219 2085 189 780 999 128 268 342
Cuts at: 5836 6971 7652 7843 826
Size: 1135 681 191 1471
Fragments arranged by size:
2085 1471 1135 999 780 681 342 268
219 191 189 128
SfoI GGC'GCC
Cuts at: 3744 3888 4704 3744
Size: 144 816 7528
Fragments arranged by size:
7528 816 144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8488
SmaI CCC'GGG
Cuts at: 1872 5815 1872
Size: 3943 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3984 4106 4574 6592 7493 7755
Size: 197 926 2041 122 468 2018 901 262
Cuts at: 7755 820
Size: 1553
Fragments arranged by size:
2041 2018 1553 926 901 468 262 197
122
SnaBI TAC'GTA
Cuts at: 493 6279 493
Size: 5786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8488
SphI G_CATG'C
Cuts at: 1132 5846 1132
Size: 4714 3774
SspI AAT'ATT
Cuts at: 5 52 2542 6412 5
Size: 47 2490 3870 2081
Fragments arranged by size:
3870 2490 2081 47
StuI AGG'CCT
Cuts at: 1517 5096 1517
Size: 3579 4909
StyI C'CwwG_G
Cuts at: 513 1065 2004 2190 3559 4050 4489 4518 4853
Size: 552 939 186 1369 491 439 29 335
Cuts at: 4853 5596 5663 5927 513
Size: 743 67 264 3074
Fragments arranged by size:
3074 1369 939 743 552 491 439 335
264 186 67 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2316 3230 5275 6515 6532 6685 6870
Size: 1255 736 914 2045 1240 17 153 185
Cuts at: 6870 7315 325
Size: 445 1498
Fragments arranged by size:
2045 1498 1255 1240 914 736 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6734 8446 96
Size: 274 80 33 51 1443 4757 1712 138
Fragments arranged by size:
4757 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3797 3825 3829 3857 4771 4803 4907 4939 8448
Size: 28 4 28 914 32 104 32 3509
Cuts at: 8448 8480 3797
Size: 32 3805
Fragments arranged by size:
3805 3509 914 104 32 32 32 28
28 4
Tth111I GACn'n_nGTC
Cuts at: 1316 3178 3251 1316
Size: 1862 73 6553
Fragments arranged by size:
6553 1862 73
XbaI T'CTAG_A
Cuts at: 5824 5824
Size: 8488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3335 4522 4603 3335
Size: 1187 81 7220
Fragments arranged by size:
7220 1187 81
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8488
XmaI C'CCGG_G
Cuts at: 1870 5813 1870
Size: 3943 4545
XmnI GAAnn'nnTTC
Cuts at: 2415 5285 6617 2415
Size: 2870 1332 4286
Fragments arranged by size:
4286 2870 1332
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2197 3823 6296 276
Size: 53 83 186 936 663 1626 2473 2468
Fragments arranged by size:
2473 2468 1626 936 663 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I DraIII
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PspXI PsrI RsrII SexAI SfiI SrfI SwaI