Restriction map for
pFN21AA0625
(Circular) (Six-base) MAPSORT of: pFN21AA0625.seq Check: 8507 from: 1 to: 12736
pFN21AA0625 12736 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3896 10546 278
Size: 53 83 186 936 2360 6650 2468
Fragments arranged by size:
6650 2468 2360 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2274 3072 7942 9117 10079 1937
Size: 337 798 4870 1175 962 4594
Fragments arranged by size:
4870 4594 1175 962 798 337
Acc65I G'GTAC_C
Cuts at: 10057 10057
Size: 12736
AclI AA'CG_TT
Cuts at: 1563 10863 11236 1563
Size: 9300 373 3063
Fragments arranged by size:
9300 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3167 3339 4128 4704 5286 5409 5535 6032
Size: 1535 172 789 576 582 123 126 497
Cuts at: 6032 6369 6534 6732 6840 8512 9596 9650 10799
Size: 337 165 198 108 1672 1084 54 1149
Cuts at: 10799 12183 12451 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1672 1535 1384 1149 1084 789 582
576 497 337 268 198 172 165 126
123 108 54
AfeI AGC'GCT
Cuts at: 2063 2063
Size: 12736
AflII C'TTAA_G
Cuts at: 820 1017 3266 7795 820
Size: 197 2249 4529 5761
Fragments arranged by size:
5761 4529 2249 197
AflIII A'CryG_T
Cuts at: 1203 2012 2386 2685 3147 4235 4381 9167 9854
Size: 809 374 299 462 1088 146 4786 687
Cuts at: 9854 11635 12612 1203
Size: 1781 977 1327
Fragments arranged by size:
4786 1781 1327 1088 977 809 687 462
374 299 146
AgeI A'CCGG_T
Cuts at: 11567 11567
Size: 12736
AhdI GACnn_n'nnGTC
Cuts at: 11465 11465
Size: 12736
AleI CACnn'nnGTG
Cuts at: 1166 3818 1166
Size: 2652 10084
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 8397 9792 1184
Size: 7213 1395 4128
Fragments arranged by size:
7213 4128 1395
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9074 9106 9074
Size: 32 12704
AlwNI CAG_nnn'CTG
Cuts at: 3350 5060 6004 6176 6302 9754 10479 12051 3350
Size: 1710 944 172 126 3452 725 1572 4035
Fragments arranged by size:
4035 3452 1710 1572 944 725 172 126
ApaI G_GGCC'C
Cuts at: 7167 7167
Size: 12736
ApaLI G'TGCA_C
Cuts at: 8022 10793 11949 8022
Size: 2771 1156 8809
Fragments arranged by size:
8809 2771 1156
ApoI r'AATT_y
Cuts at: 1493 2090 3371 3709 3791 4444 4667 5456 5822
Size: 597 1281 338 82 653 223 789 366
Cuts at: 5822 6151 6964 7315 7340 7532 8072 8128 8258
Size: 329 813 351 25 192 540 56 130
Cuts at: 8258 10045 10271 10423 12532 1493
Size: 1787 226 152 2109 1697
Fragments arranged by size:
2109 1787 1697 1281 813 789 653 597
540 366 351 338 329 226 223 192
152 130 82 56 25
AseI AT'TA_AT
Cuts at: 160 1032 5987 8160 11290 160
Size: 872 4955 2173 3130 1606
Fragments arranged by size:
4955 3130 2173 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12736
AvaI C'yCGr_G
Cuts at: 1870 1943 9685 9842 10061 1870
Size: 73 7742 157 219 4545
Fragments arranged by size:
7742 4545 219 157 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4479 4512 4479
Size: 33 12703
BamHI G'GATC_C
Cuts at: 2854 2932 9673 10066 10433 2854
Size: 78 6741 393 367 5157
Fragments arranged by size:
6741 5157 393 367 78
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 9338 10057 11512 618
Size: 325 217 627 7551 719 1455 1842
Fragments arranged by size:
7551 1842 1455 719 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2783 7167 8302 9692 9891 10055 1391
Size: 1392 4384 1135 1390 199 164 4072
Fragments arranged by size:
4384 4072 1392 1390 1135 199 164
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2115 3896 5664 6100 6396 9065 928
Size: 971 216 1781 1768 436 296 2669 4599
Fragments arranged by size:
4599 2669 1781 1768 971 436 296 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3049 3083 8286 8320 10927 10961 1903
Size: 34 1112 34 5203 34 2607 34 3678
Fragments arranged by size:
5203 3678 2607 1112 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6888 10628 11838 6888
Size: 3740 1210 7786
Fragments arranged by size:
7786 3740 1210
BclI T'GATC_A
Cuts at: 1548 3975 5938 8739 1548
Size: 2427 1963 2801 5545
Fragments arranged by size:
5545 2801 2427 1963
BfrBI ATG'CAT
Cuts at: 3910 6252 8245 8785 9131 3910
Size: 2342 1993 540 346 7515
Fragments arranged by size:
7515 2342 1993 540 346
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 11347 136
Size: 107 122 71 631 605 9675 1525
Fragments arranged by size:
9675 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4155 4747 5578 7498 12731 4155
Size: 592 831 1920 5233 4160
Fragments arranged by size:
5233 4160 1920 831 592
BlpI GC'TnA_GC
Cuts at: 10153 10153
Size: 12736
Bme1580I G_kGCm'C
Cuts at: 1790 7167 8026 10797 11953 1790
Size: 5377 859 2771 1156 2573
Fragments arranged by size:
5377 2771 2573 1156 859
BmgBI CAC'GTC
Cuts at: 1318 6422 7545 1318
Size: 5104 1123 6509
Fragments arranged by size:
6509 5104 1123
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2319 7375 8150
Size: 416 53 83 399 24 902 5056 775
Cuts at: 8150 10467 11425 442
Size: 2317 958 1753
Fragments arranged by size:
5056 2317 1753 958 902 775 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12736
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6478 6478
Size: 12736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6279 6448 7047 9723 9813 10016 11396 1239
Size: 5040 169 599 2676 90 203 1380 2579
Fragments arranged by size:
5040 2676 2579 1380 599 203 169 90
Bpu10I CC'TnA_GC
Cuts at: 5838 8628 9755 5838
Size: 2790 1127 8819
Fragments arranged by size:
8819 2790 1127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2768 4896 5114 5147 7177 7578 8513 9383 10861
Size: 2128 218 33 2030 401 935 870 1478
Cuts at: 10861 11726 12024 2768
Size: 865 298 3480
Fragments arranged by size:
3480 2128 2030 1478 935 870 865 401
298 218 33
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4878 6297 6775 9944 11399 882
Size: 617 3379 1419 478 3169 1455 2219
Fragments arranged by size:
3379 3169 2219 1455 1419 617 478
BsaAI yAC'GTr
Cuts at: 493 1204 5636 5936 9168 9855 10527 493
Size: 711 4432 300 3232 687 672 2702
Fragments arranged by size:
4432 3232 2702 711 687 672 300
BsaBI GATnn'nnATC
Cuts at: 2460 8869 12730 2460
Size: 6409 3861 2466
Fragments arranged by size:
6409 3861 2466
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3893 8410 10543 10925
Size: 53 83 186 936 2360 4517 2133 382
Cuts at: 10925 11560 275
Size: 635 1451
Fragments arranged by size:
4517 2360 2133 1451 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2084 3028 11169 11567 11841 11988 12399 2084
Size: 944 8141 398 274 147 411 2421
Fragments arranged by size:
8141 2421 944 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5608 5638 7485 7515 7967
Size: 30 165 30 4205 30 1847 30 452
Cuts at: 7967 7997 8566 8596 9415 9445 1178
Size: 30 569 30 819 30 4469
Fragments arranged by size:
4469 4205 1847 819 569 452 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4793 5985 6750 7922 9899 1186
Size: 3607 1192 765 1172 1977 4023
Fragments arranged by size:
4023 3607 1977 1192 1172 765
BseYI C'CCAG_C
Cuts at: 3450 4058 5904 9476 9693 11939 12435 3450
Size: 608 1846 3572 217 2246 496 3751
Fragments arranged by size:
3751 3572 2246 1846 608 496 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3632 8446 1163
Size: 573 1896 4814 5453
Fragments arranged by size:
5453 4814 1896 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 10186 10947 11975 1540
Size: 275 186 8185 761 1028 2301
Fragments arranged by size:
8185 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 8026 8302 8474 9891 10797 10882 11953 2012
Size: 6014 276 172 1417 906 85 1071 2795
Fragments arranged by size:
6014 2795 1417 1071 906 276 172 85
BsmI GAATG_Cn'
Cuts at: 3574 7969 9131 10247 10340 3574
Size: 4395 1162 1116 93 5970
Fragments arranged by size:
5970 4395 1162 1116 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2213 6876 2213
Size: 4663 8073
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2783 7167 8026 8302 8474 9692
Size: 399 222 771 4384 859 276 172 1218
Cuts at: 9692 9891 10055 10797 10882 11953 1391
Size: 199 164 742 85 1071 2174
Fragments arranged by size:
4384 2174 1218 1071 859 771 742 399
276 222 199 172 164 85
BspEI T'CCGG_A
Cuts at: 12399 12399
Size: 12736
BspHI T'CATG_A
Cuts at: 8052 9601 10625 12355 8052
Size: 1549 1024 1730 8433
Fragments arranged by size:
8433 1730 1549 1024
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 10091 844
Size: 9247 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 10623 1394
Size: 429 8800 3507
Fragments arranged by size:
8800 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3461 3558 3569 6979 11231 11405 65
Size: 1183 2213 97 11 3410 4252 174 1396
Fragments arranged by size:
4252 3410 2213 1396 1183 174 97 11
BsrFI r'CCGG_y
Cuts at: 1700 1810 11380 11567 1700
Size: 110 9570 187 2869
Fragments arranged by size:
9570 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3595 5372 5690 6197 7748 96
Size: 3499 1777 318 507 1551 5084
Fragments arranged by size:
5084 3499 1777 1551 507 318
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12736
BssSI C'ACGA_G
Cuts at: 8015 9243 10796 11808 8015
Size: 1228 1553 1012 8943
Fragments arranged by size:
8943 1553 1228 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3580 794
Size: 2786 9950
BstBI TT'CG_AA
Cuts at: 9022 11540 9022
Size: 2518 10218
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 12736
BstXI CCAn_nnnn'nTGG
Cuts at: 2500 9701 2500
Size: 7201 5535
BstYI r'GATC_y
Cuts at: 1972 2469 2854 2906 2932 2985 3262 3823 4155
Size: 497 385 52 26 53 277 561 332
Cuts at: 4155 4747 5518 5578 7498 8623 9673 10066 10433
Size: 592 771 60 1920 1125 1050 393 367
Cuts at: 10433 10818 10835 12287 12373 12385 12731 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1920 1452 1125 1050 771 592 561
497 393 385 385 367 346 332 277
86 60 53 52 26 17 12
BstZ17I GTA'TAC
Cuts at: 3073 3073
Size: 12736
BtgI C'CryG_G
Cuts at: 513 1065 2760 5032 5435 8506 513
Size: 552 1695 2272 403 3071 4743
Fragments arranged by size:
4743 3071 2272 1695 552 403
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4941 8255 8579 487
Size: 683 56 3715 3314 324 4644
Fragments arranged by size:
4644 3715 3314 683 324 56
BtsI GCAGTG_nn'
Cuts at: 3495 6475 9387 10252 11046 11066 3495
Size: 2980 2912 865 794 20 5165
Fragments arranged by size:
5165 2980 2912 865 794 20
ClaI AT'CG_AT
Cuts at: 1554 2810 1554
Size: 1256 11480
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 12701
DraI TTT'AAA
Cuts at: 2596 4305 4744 5599 7617 10040 2596
Size: 1709 439 855 2018 2423 5292
Fragments arranged by size:
5292 2423 2018 1709 855 439
DraIII CAC_nnn'GTG
Cuts at: 6648 6648
Size: 12736
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 11743 809
Size: 507 10427 1802
Fragments arranged by size:
10427 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2864 7608 8879
Size: 54 1425 50 275 18 1034 4744 1271
Cuts at: 8879 10183 11072 12701 8
Size: 1304 889 1629 43
Fragments arranged by size:
4744 1629 1425 1304 1271 1034 889 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 10183 1537
Size: 275 8371 4090
Fragments arranged by size:
8371 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2349 4165 4400 7458 8712 8807 9266 9626
Size: 996 1816 235 3058 1254 95 459 360
Cuts at: 9626 9876 10666 1353
Size: 250 790 3423
Fragments arranged by size:
3423 3058 1816 1254 996 790 459 360
250 235 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 11323 11697 11843 387
Size: 1064 138 9734 374 146 1280
Fragments arranged by size:
9734 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 8300 9889 8300
Size: 1589 11147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3167 3339 4128 4704 5286 5409 5535
Size: 393 1535 172 789 576 582 123 126
Cuts at: 5535 6032 6279 6369 6448 6534 6732 6840 7047
Size: 497 247 90 79 86 198 108 207
Cuts at: 7047 8512 9596 9650 9723 9813 10016 10799 11396
Size: 1465 1084 54 73 90 203 783 597
Cuts at: 11396 12183 12451 1239
Size: 787 268 1524
Fragments arranged by size:
1535 1524 1465 1084 789 787 783 597
582 576 497 393 268 247 207 203
198 172 126 123 108 90 90 86
79 73 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2654 5873 7902 2654
Size: 3219 2029 7488
Fragments arranged by size:
7488 3219 2029
EcoO109I rG'GnC_Cy
Cuts at: 4831 5879 7164 9282 9580 9616 9751 4831
Size: 1048 1285 2118 298 36 135 7816
Fragments arranged by size:
7816 2118 1285 1048 298 135 36
EcoRI G'AATT_C
Cuts at: 6151 6964 8072 10045 10423 6151
Size: 813 1108 1973 378 8464
Fragments arranged by size:
8464 1973 1108 813 378
EcoRV GAT'ATC
Cuts at: 3558 3558
Size: 12736
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2600 3709 6683 2600
Size: 1109 2974 8653
Fragments arranged by size:
8653 2974 1109
FspI TGC'GCA
Cuts at: 11242 11242
Size: 12736
HaeII r_GCGC'y
Cuts at: 1678 2065 11883 1678
Size: 387 9818 2531
Fragments arranged by size:
9818 2531 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2881 2898 2913 2930 3509
Size: 32 135 32 1309 17 15 17 579
Cuts at: 3509 3541 3610 3642 4715 4747 5122 5154 5194
Size: 32 69 32 1073 32 375 32 40
Cuts at: 5194 5226 5369 5401 5543 5575 5608 5640 7967
Size: 32 143 32 142 32 33 32 2327
Cuts at: 7967 7999 8566 8598 8924 8956 9216 9248 11377
Size: 32 567 32 326 32 260 32 2129
Cuts at: 11377 11409 11451 11483 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2327 2129 1309 1073 579 567 375
326 260 143 142 135 69 42 40
33 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2392 2924 3732 4245 10080 10326
Size: 988 281 454 532 808 513 5835 246
Cuts at: 10326 669
Size: 3079
Fragments arranged by size:
5835 3079 988 808 532 513 454 281
246
HindIII A'AGCT_T
Cuts at: 748 2330 3485 3921 5678 8987 10028 748
Size: 1582 1155 436 1757 3309 1041 3456
Fragments arranged by size:
3456 3309 1757 1582 1155 1041 436
HpaI GTT'AAC
Cuts at: 2392 10326 2392
Size: 7934 4802
KpnI G_GTAC'C
Cuts at: 10061 10061
Size: 12736
MfeI C'AATT_G
Cuts at: 2434 4651 9198 10335 2434
Size: 2217 4547 1137 4835
Fragments arranged by size:
4835 4547 2217 1137
MluI A'CGCG_T
Cuts at: 12612 12612
Size: 12736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2097 2399 3013 4999 5935 6437 6606 7029
Size: 792 302 614 1986 936 502 169 423
Cuts at: 7029 7679 8666 8890 11850 12034 1305
Size: 650 987 224 2960 184 2007
Fragments arranged by size:
2960 2007 1986 987 936 792 650 614
502 423 302 224 184 169
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2866 8881 10
Size: 54 1425 343 1034 6015 3865
Fragments arranged by size:
6015 3865 1425 1034 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2385 2493 2688 3229 3805 3818 4677
Size: 648 1219 108 195 541 576 13 859
Cuts at: 4677 5745 7221 7288 8654 10694 11053 11212 518
Size: 1068 1476 67 1366 2040 359 159 2042
Fragments arranged by size:
2042 2040 1476 1366 1219 1068 859 648
576 541 359 195 159 108 67 13
MspA1I CmG'CkG
Cuts at: 1606 3616 4963 5855 5908 8039 8275 8532 9313
Size: 2010 1347 892 53 2131 236 257 781
Cuts at: 9313 9776 9833 10152 10829 11977 12222 1606
Size: 463 57 319 677 1148 245 2120
Fragments arranged by size:
2131 2120 2010 1347 1148 892 781 677
463 319 257 245 236 57 53
NcoI C'CATG_G
Cuts at: 513 1065 2760 513
Size: 552 1695 10489
Fragments arranged by size:
10489 1695 552
NdeI CA'TA_TG
Cuts at: 387 5893 7840 8795 387
Size: 5506 1947 955 4328
Fragments arranged by size:
5506 4328 1947 955
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12736
NotI GC'GGCC_GC
Cuts at: 10183 10183
Size: 12736
NsiI A_TGCA'T
Cuts at: 3912 6254 8247 8787 9133 3912
Size: 2342 1993 540 346 7515
Fragments arranged by size:
7515 2342 1993 540 346
NspI r_CATG'y
Cuts at: 1132 1229 2016 2689 2734 3151 3208 3840 4385
Size: 97 787 673 45 417 57 632 545
Cuts at: 4385 5153 6256 10094 11639 1132
Size: 768 1103 3838 1545 2229
Fragments arranged by size:
3838 2229 1545 1103 787 768 673 632
545 417 97 57 45
PasI CC'CwG_GG
Cuts at: 1348 9463 1348
Size: 8115 4621
PciI A'CATG_T
Cuts at: 2012 2685 3147 4381 11635 2012
Size: 673 462 1234 7254 3113
Fragments arranged by size:
7254 3113 1234 673 462
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 12736
PfoI T'CCnGG_A
Cuts at: 9700 9700
Size: 12736
PmeI GTTT'AAAC
Cuts at: 10040 10040
Size: 12736
PmlI CAC'GTG
Cuts at: 9855 9855
Size: 12736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10805 10837 12318 12350 10805
Size: 32 1481 32 11191
Fragments arranged by size:
11191 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5879 9282 9580 9616 9751 5879
Size: 3403 298 36 135 8864
Fragments arranged by size:
8864 3403 298 135 36
PshAI GACnn'nnGTC
Cuts at: 1613 4842 1613
Size: 3229 9507
PsiI TTA'TAA
Cuts at: 3861 6192 6555 8823 10306 3861
Size: 2331 363 2268 1483 6291
Fragments arranged by size:
6291 2331 2268 1483 363
PspOMI G'GGCC_C
Cuts at: 7163 7163
Size: 12736
PspXI vC'TCGA_Gb
Cuts at: 9685 9685
Size: 12736
PstI C_TGCA'G
Cuts at: 830 8708 9414 10088 830
Size: 7878 706 674 3478
Fragments arranged by size:
7878 3478 706 674
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12736
PvuII CAG'CTG
Cuts at: 3616 4963 5855 8039 8275 8532 9313 9833 3616
Size: 1347 892 2184 236 257 781 520 6519
Fragments arranged by size:
6519 2184 1347 892 781 520 257 236
SacI G_AGCT'C
Cuts at: 8302 9891 8302
Size: 1589 11147
SalI G'TCGA_C
Cuts at: 1936 10078 1936
Size: 8142 4594
SbfI CC_TGCA'GG
Cuts at: 10088 10088
Size: 12736
ScaI AGT'ACT
Cuts at: 4411 6814 10984 12696 4411
Size: 2403 4170 1712 4451
Fragments arranged by size:
4451 4170 2403 1712
SfcI C'TryA_G
Cuts at: 826 1045 3993 5343 5805 6336 8704 8759 9367
Size: 219 2948 1350 462 531 2368 55 608
Cuts at: 9367 9410 9780 10084 11219 11900 12091 826
Size: 43 370 304 1135 681 191 1471
Fragments arranged by size:
2948 2368 1471 1350 1135 681 608 531
462 370 304 219 191 55 43
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12736
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12736
SmaI CCC'GGG
Cuts at: 1872 10063 1872
Size: 8191 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2747 3266 4911 5093 5126 7192
Size: 197 926 804 519 1645 182 33 2066
Cuts at: 7192 7593 7795 8492 9398 9685 10840 11741 12003
Size: 401 202 697 906 287 1155 901 262
Cuts at: 12003 820
Size: 1553
Fragments arranged by size:
2066 1645 1553 1155 926 906 901 804
697 519 401 287 262 202 197 182
33
SnaBI TAC'GTA
Cuts at: 493 10527 493
Size: 10034 2702
SpeI A'CTAG_T
Cuts at: 152 3734 6035 152
Size: 3582 2301 6853
Fragments arranged by size:
6853 3582 2301
SphI G_CATG'C
Cuts at: 1132 6256 10094 1132
Size: 5124 3838 3774
Fragments arranged by size:
5124 3838 3774
SspI AAT'ATT
Cuts at: 5 52 7046 9663 10660 5
Size: 47 6994 2617 997 2081
Fragments arranged by size:
6994 2617 2081 997 47
StuI AGG'CCT
Cuts at: 1517 5530 1517
Size: 4013 8723
StyI C'CwwG_G
Cuts at: 513 1065 2760 2902 9190 9549 10175 513
Size: 552 1695 142 6288 359 626 3074
Fragments arranged by size:
6288 3074 1695 626 552 359 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3266 3448 6389 10763 10780 10933 11118
Size: 1255 1686 182 2941 4374 17 153 185
Cuts at: 11118 11563 325
Size: 445 1498
Fragments arranged by size:
4374 2941 1686 1498 1255 445 185 182
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3178 3312 3595
Size: 274 80 33 51 1443 1201 134 283
Cuts at: 3595 4127 4181 4409 5372 5684 5690 6113 6197
Size: 532 54 228 963 312 6 423 84
Cuts at: 6197 6654 6812 7748 8775 9252 10982 12694 96
Size: 457 158 936 1027 477 1730 1712 138
Fragments arranged by size:
1730 1712 1443 1201 1027 963 936 532
477 457 423 312 283 274 228 158
138 134 84 80 54 51 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9415 9447 12696 12728 9415
Size: 32 3249 32 9423
Fragments arranged by size:
9423 3249 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3362 8142 1316
Size: 2046 4780 5910
Fragments arranged by size:
5910 4780 2046
XbaI T'CTAG_A
Cuts at: 3896 8263 10072 3896
Size: 4367 1809 6560
Fragments arranged by size:
6560 4367 1809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4055 5015 7687 9197 4055
Size: 960 2672 1510 7594
Fragments arranged by size:
7594 2672 1510 960
XhoI C'TCGA_G
Cuts at: 1943 9685 1943
Size: 7742 4994
XmaI C'CCGG_G
Cuts at: 1870 10061 1870
Size: 8191 4545
XmnI GAAnn'nnTTC
Cuts at: 2502 3375 5617 6097 6570 8859 10865 2502
Size: 873 2242 480 473 2289 2006 4373
Fragments arranged by size:
4373 2289 2242 2006 873 480 473
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3894 10544 276
Size: 53 83 186 936 2360 6650 2468
Fragments arranged by size:
6650 2468 2360 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BbeI BbvCI BsiWI Bsu36I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI