Restriction map for
pFN21AA0622
(Circular) (Six-base) MAPSORT of: pFN21AA0622.seq Check: 7924 from: 1 to: 9094
pFN21AA0622 9094 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2026 2026
Size: 9094
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6904 278
Size: 53 83 186 936 5368 2468
Fragments arranged by size:
5368 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6168 6437 1937
Size: 4231 269 4594
Fragments arranged by size:
4594 4231 269
Acc65I G'GTAC_C
Cuts at: 6415 6415
Size: 9094
AclI AA'CG_TT
Cuts at: 1563 2811 7221 7594 1563
Size: 1248 4410 373 3063
Fragments arranged by size:
4410 3063 1248 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2850 3246 3538 3644 4128 4565 5325 7157
Size: 1218 396 292 106 484 437 760 1832
Cuts at: 7157 8541 8809 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1832 1384 1218 760 484 437 396
292 268 106
AfeI AGC'GCT
Cuts at: 4409 4409
Size: 9094
AflII C'TTAA_G
Cuts at: 820 1017 4727 820
Size: 197 3710 5187
Fragments arranged by size:
5187 3710 197
AflIII A'CryG_T
Cuts at: 1203 2593 2605 7993 8970 1203
Size: 1390 12 5388 977 1327
Fragments arranged by size:
5388 1390 1327 977 12
AgeI A'CCGG_T
Cuts at: 7925 7925
Size: 9094
AhdI GACnn_n'nnGTC
Cuts at: 2224 3145 7823 2224
Size: 921 4678 3495
Fragments arranged by size:
4678 3495 921
AleI CACnn'nnGTG
Cuts at: 1166 5159 1166
Size: 3993 5101
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2297 2525 6071 1184
Size: 1113 228 3546 4207
Fragments arranged by size:
4207 3546 1113 228
AlwNI CAG_nnn'CTG
Cuts at: 6837 8409 6837
Size: 1572 7522
ApaI G_GGCC'C
Cuts at: 6343 6343
Size: 9094
ApaLI G'TGCA_C
Cuts at: 7151 8307 7151
Size: 1156 7938
ApoI r'AATT_y
Cuts at: 1493 2422 2583 2669 2722 3401 5066 5143 5895
Size: 929 161 86 53 679 1665 77 752
Cuts at: 5895 6403 6629 6781 8890 1493
Size: 508 226 152 2109 1697
Fragments arranged by size:
2109 1697 1665 929 752 679 508 226
161 152 86 77 53
AseI AT'TA_AT
Cuts at: 160 1032 7648 160
Size: 872 6616 1606
Fragments arranged by size:
6616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9094
AvaI C'yCGr_G
Cuts at: 1870 1943 2433 5548 6419 1870
Size: 73 490 3115 871 4545
Fragments arranged by size:
4545 3115 871 490 73
AvrII C'CTAG_G
Cuts at: 3332 3332
Size: 9094
BamHI G'GATC_C
Cuts at: 2855 3921 4924 6424 6791 2855
Size: 1066 1003 1500 367 5158
Fragments arranged by size:
5158 1500 1066 1003 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5084 5111 6415 7870 618
Size: 325 217 627 3297 27 1304 1455 1842
Fragments arranged by size:
3297 1842 1455 1304 627 325 217 27
BanII G_rGCy'C
Cuts at: 1391 4115 5168 5752 6343 6413 1391
Size: 2724 1053 584 591 70 4072
Fragments arranged by size:
4072 2724 1053 591 584 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2856 4134 5787 5957 6276 928
Size: 971 957 1278 1653 170 319 3746
Fragments arranged by size:
3746 1653 1278 971 957 319 170
BbvCI CC'TCA_GC
Cuts at: 6000 6000
Size: 9094
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7285 7319 1903
Size: 34 5348 34 3678
Fragments arranged by size:
5348 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6986 8196 6986
Size: 1210 7884
BclI T'GATC_A
Cuts at: 1548 4813 1548
Size: 3265 5829
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7705 136
Size: 107 122 71 631 605 6033 1525
Fragments arranged by size:
6033 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2617 3050 4028 4291 4593 5374 9089 2617
Size: 433 978 263 302 781 3715 2622
Fragments arranged by size:
3715 2622 978 781 433 302 263
BlpI GC'TnA_GC
Cuts at: 6511 6511
Size: 9094
Bme1580I G_kGCm'C
Cuts at: 1790 3863 5670 6343 7155 8311 1790
Size: 2073 1807 673 812 1156 2573
Fragments arranged by size:
2573 2073 1807 1156 812 673
BmgBI CAC'GTC
Cuts at: 1318 5011 5665 1318
Size: 3693 654 4747
Fragments arranged by size:
4747 3693 654
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2374 3544 3789
Size: 416 53 83 399 24 957 1170 245
Cuts at: 3789 4116 6825 7783 442
Size: 327 2709 958 1753
Fragments arranged by size:
2709 1753 1170 958 957 416 399 327
245 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2499 3773 3879 4405 5777 5855 7754 1239
Size: 1260 1274 106 526 1372 78 1899 2579
Fragments arranged by size:
2579 1899 1372 1274 1260 526 106 78
Bpu10I CC'TnA_GC
Cuts at: 3135 6000 3135
Size: 2865 6229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 2984 3666 4375 4856 5123 5750 7219 8084
Size: 840 682 709 481 267 627 1469 865
Cuts at: 8084 8382 2144
Size: 298 2856
Fragments arranged by size:
2856 1469 865 840 709 682 627 481
298 267
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2952 3153 5831 7757 882
Size: 617 1453 201 2678 1926 2219
Fragments arranged by size:
2678 2219 1926 1453 617 201
BsaAI yAC'GTr
Cuts at: 493 1204 2386 5327 6885 493
Size: 711 1182 2941 1558 2702
Fragments arranged by size:
2941 2702 1558 1182 711
BsaBI GATnn'nnATC
Cuts at: 4027 9088 4027
Size: 5061 4033
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3866 5192 6901 7283
Size: 53 83 186 936 2333 1326 1709 382
Cuts at: 7283 7918 275
Size: 635 1451
Fragments arranged by size:
2333 1709 1451 1326 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 7527 7925 8199 8346 8757 7527
Size: 398 274 147 411 7864
Fragments arranged by size:
7864 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2592 2622 4846 4876 5518
Size: 30 165 30 1189 30 2224 30 642
Cuts at: 5518 5548 5957 5987 5996 6026 6350 6380 1178
Size: 30 409 30 9 30 324 30 3892
Fragments arranged by size:
3892 2224 1189 642 409 324 165 30
30 30 30 30 30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3838 4105 4348 4351 5816 6017 6358 6361
Size: 2652 267 243 3 1465 201 341 3
Cuts at: 6361 1186
Size: 3919
Fragments arranged by size:
3919 2652 1465 341 267 243 201 3
3
BseYI C'CCAG_C
Cuts at: 2209 4017 5529 8297 8793 2209
Size: 1808 1512 2768 496 2510
Fragments arranged by size:
2768 2510 1808 1512 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5111 1163
Size: 573 3375 5146
Fragments arranged by size:
5146 3375 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6081 6544 7305 8333 1540
Size: 275 186 4080 463 761 1028 2301
Fragments arranged by size:
4080 2301 1028 761 463 275 186
BsiHKAI G_wGCw'C
Cuts at: 2485 3573 4283 5808 5872 6060 7155 7240 8311
Size: 1088 710 1525 64 188 1095 85 1071
Cuts at: 8311 2485
Size: 3268
Fragments arranged by size:
3268 1525 1095 1088 1071 710 188 85
64
BsmI GAATG_Cn'
Cuts at: 2403 4267 4434 6605 6698 2403
Size: 1864 167 2171 93 4799
Fragments arranged by size:
4799 2171 1864 167 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5655 5655
Size: 9094
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2485 3573 3863 4115 4283 5168 5670
Size: 399 695 1088 290 252 168 885 502
Cuts at: 5670 5752 5808 5872 6060 6343 6413 7155 7240
Size: 82 56 64 188 283 70 742 85
Cuts at: 7240 8311 1391
Size: 1071 2174
Fragments arranged by size:
2174 1088 1071 885 742 695 502 399
290 283 252 188 168 85 82 70
64 56
BspEI T'CCGG_A
Cuts at: 8757 8757
Size: 9094
BspHI T'CATG_A
Cuts at: 6983 8713 6983
Size: 1730 7364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2026 2059 3150 6449 844
Size: 1182 33 1091 3299 3489
Fragments arranged by size:
3489 3299 1182 1091 33
BsrBI CCG'CTC
Cuts at: 1394 1823 3666 5996 6981 1394
Size: 429 1843 2330 985 3507
Fragments arranged by size:
3507 2330 1843 985 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6149 7589 7763 65
Size: 1183 4901 1440 174 1396
Fragments arranged by size:
4901 1440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2832 4056 7738 7925 1700
Size: 110 1022 1224 3682 187 2869
Fragments arranged by size:
3682 2869 1224 1022 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9094
BssHII G'CGCG_C
Cuts at: 1925 5539 1925
Size: 3614 5480
BssSI C'ACGA_G
Cuts at: 4119 7154 8166 4119
Size: 3035 1012 5047
Fragments arranged by size:
5047 3035 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2041 3830 794
Size: 1247 1789 6058
Fragments arranged by size:
6058 1789 1247
BstBI TT'CG_AA
Cuts at: 5362 7898 5362
Size: 2536 6558
BstEII G'GTnAC_C
Cuts at: 1184 2236 1184
Size: 1052 8042
BstXI CCAn_nnnn'nTGG
Cuts at: 2690 3277 4921 2690
Size: 587 1644 6863
Fragments arranged by size:
6863 1644 587
BstYI r'GATC_y
Cuts at: 1972 2536 2617 2855 3050 3921 4028 4291 4593
Size: 564 81 238 195 871 107 263 302
Cuts at: 4593 4754 4924 5374 6424 6791 7176 7193 8645
Size: 161 170 450 1050 367 385 17 1452
Cuts at: 8645 8731 8743 9089 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1050 871 564 450 385 367
346 302 263 238 195 170 161 107
86 81 17 12
Bsu36I CC'TnA_GG
Cuts at: 3913 3913
Size: 9094
BtgI C'CryG_G
Cuts at: 513 1065 2004 3217 5251 5555 513
Size: 552 939 1213 2034 304 4052
Fragments arranged by size:
4052 2034 1213 939 552 304
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5445 487
Size: 683 56 4219 4136
Fragments arranged by size:
4219 4136 683 56
BtsI GCAGTG_nn'
Cuts at: 6050 6610 7404 7424 6050
Size: 560 794 20 7720
Fragments arranged by size:
7720 794 560 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9059
DraI TTT'AAA
Cuts at: 3124 5923 6398 3124
Size: 2799 475 5820
Fragments arranged by size:
5820 2799 475
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8101 809
Size: 507 6785 1802
Fragments arranged by size:
6785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2074 3279 4231
Size: 54 1425 50 275 18 244 1205 952
Cuts at: 4231 4911 5185 6021 6078 6541 7430 9059 8
Size: 680 274 836 57 463 889 1629 43
Fragments arranged by size:
1629 1425 1205 952 889 836 680 463
275 274 244 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6078 6541 1537
Size: 275 4266 463 4090
Fragments arranged by size:
4266 4090 463 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2895 4541 7024 1353
Size: 1542 1646 2483 3423
Fragments arranged by size:
3423 2483 1646 1542
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2218 7681 8055 8201 387
Size: 1064 138 629 5463 374 146 1280
Fragments arranged by size:
5463 1280 1064 629 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2499 2850 3246 3538 3644 3773 3879
Size: 393 867 351 396 292 106 129 106
Cuts at: 3879 4128 4405 4565 5325 5777 5855 7157 7754
Size: 249 277 160 760 452 78 1302 597
Cuts at: 7754 8541 8809 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1302 867 787 760 597 452 396
393 351 292 277 268 249 160 129
106 106 78
EcoNI CCTnn'n_nnAGG
Cuts at: 2033 5624 2033
Size: 3591 5503
EcoO109I rG'GnC_Cy
Cuts at: 3139 3783 6339 3139
Size: 644 2556 5894
Fragments arranged by size:
5894 2556 644
EcoRI G'AATT_C
Cuts at: 5143 6403 6781 5143
Size: 1260 378 7456
Fragments arranged by size:
7456 1260 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4538 4718 5804 4538
Size: 180 1086 7828
Fragments arranged by size:
7828 1086 180
FspI TGC'GCA
Cuts at: 7600 7600
Size: 9094
HaeII r_GCGC'y
Cuts at: 1678 3394 4411 5442 8241 1678
Size: 1716 1017 1031 2799 2531
Fragments arranged by size:
2799 2531 1716 1031 1017
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2210 2242 2347
Size: 32 135 32 509 32 97 32 105
Cuts at: 2347 2379 2427 2459 2602 2634 3131 3163 3653
Size: 32 48 32 143 32 497 32 490
Cuts at: 3653 3679 3685 3711 4585 4617 5408 5440 5996
Size: 26 6 26 874 32 791 32 556
Cuts at: 5996 6028 7735 7767 7809 7841 1373
Size: 32 1707 32 42 32 2626
Fragments arranged by size:
2626 1707 874 791 556 509 497 490
143 135 105 97 48 42 32 32
32 32 32 32 32 32 32 32
32 32 32 26 26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3774 6169 6438 6684 669
Size: 988 281 1836 2395 269 246 3079
Fragments arranged by size:
3079 2395 1836 988 281 269 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9094
HpaI GTT'AAC
Cuts at: 6684 6684
Size: 9094
KpnI G_GTAC'C
Cuts at: 6419 6419
Size: 9094
MfeI C'AATT_G
Cuts at: 6693 6693
Size: 9094
MluI A'CGCG_T
Cuts at: 8970 8970
Size: 9094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2794 3899 4952 5739 8208 8392 1305
Size: 1489 1105 1053 787 2469 184 2007
Fragments arranged by size:
2469 2007 1489 1105 1053 787 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2076 3281 4233 4913 6023
Size: 54 1425 343 244 1205 952 680 1110
Cuts at: 6023 10
Size: 3081
Fragments arranged by size:
3081 1425 1205 1110 952 680 343 244
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2518 4604 4683 4832 5159 6053 6110
Size: 648 1352 2086 79 149 327 894 57
Cuts at: 6110 7052 7411 7570 518
Size: 942 359 159 2042
Fragments arranged by size:
2086 2042 1352 942 894 648 359 327
159 149 79 57
MspA1I CmG'CkG
Cuts at: 1606 2870 3703 5557 6510 7187 8335 8580 1606
Size: 1264 833 1854 953 677 1148 245 2120
Fragments arranged by size:
2120 1854 1264 1148 953 833 677 245
NaeI GCC'GGC
Cuts at: 2834 2834
Size: 9094
NcoI C'CATG_G
Cuts at: 513 1065 2004 3217 5251 513
Size: 552 939 1213 2034 4356
Fragments arranged by size:
4356 2034 1213 939 552
NdeI CA'TA_TG
Cuts at: 387 4414 387
Size: 4027 5067
NgoMIV G'CCGG_C
Cuts at: 2832 2832
Size: 9094
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9094
NotI GC'GGCC_GC
Cuts at: 6541 6541
Size: 9094
NspI r_CATG'y
Cuts at: 1132 1229 2597 5275 6452 7997 1132
Size: 97 1368 2678 1177 1545 2229
Fragments arranged by size:
2678 2229 1545 1368 1177 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9094
PciI A'CATG_T
Cuts at: 2593 7993 2593
Size: 5400 3694
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3586 1108
Size: 2478 6616
PfoI T'CCnGG_A
Cuts at: 4421 5103 4421
Size: 682 8412
PmeI GTTT'AAAC
Cuts at: 6398 6398
Size: 9094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5285 5317 5618 5650 7163 7195 8676 8708 5285
Size: 32 301 32 1513 32 1481 32 5671
Fragments arranged by size:
5671 1513 1481 301 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3139 3783 3139
Size: 644 8450
PshAI GACnn'nnGTC
Cuts at: 1613 6122 1613
Size: 4509 4585
PsiI TTA'TAA
Cuts at: 4670 6664 4670
Size: 1994 7100
PspOMI G'GGCC_C
Cuts at: 6339 6339
Size: 9094
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4458 4490 4458
Size: 32 9062
PstI C_TGCA'G
Cuts at: 830 2250 3615 3883 4536 6159 6192 6446 830
Size: 1420 1365 268 653 1623 33 254 3478
Fragments arranged by size:
3478 1623 1420 1365 653 268 254 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9094
PvuII CAG'CTG
Cuts at: 2870 2870
Size: 9094
SacII CC_GC'GG
Cuts at: 5558 5558
Size: 9094
SalI G'TCGA_C
Cuts at: 1936 6167 6436 1936
Size: 4231 269 4594
Fragments arranged by size:
4594 4231 269
SanDI GG'GwC_CC
Cuts at: 3783 3783
Size: 9094
SapI GCTCTTCn'nnn_
Cuts at: 4541 4541
Size: 9094
SbfI CC_TGCA'GG
Cuts at: 6446 6446
Size: 9094
ScaI AGT'ACT
Cuts at: 7342 9054 7342
Size: 1712 7382
SexAI A'CCwGG_T
Cuts at: 4249 5685 4249
Size: 1436 7658
SfcI C'TryA_G
Cuts at: 826 1045 2246 2934 3611 3750 3879 4532 5314
Size: 219 1201 688 677 139 129 653 782
Cuts at: 5314 6155 6188 6442 7577 8258 8449 826
Size: 841 33 254 1135 681 191 1471
Fragments arranged by size:
1471 1201 1135 841 782 688 681 677
653 254 219 191 139 129 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9094
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9094
SmaI CCC'GGG
Cuts at: 1872 5550 6421 1872
Size: 3678 871 4545
Fragments arranged by size:
4545 3678 871
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2123 2433 2963 3681 4390 4727
Size: 197 926 180 310 530 718 709 337
Cuts at: 4727 4871 5138 5765 7198 8099 8361 820
Size: 144 267 627 1433 901 262 1553
Fragments arranged by size:
1553 1433 926 901 718 709 627 530
337 310 267 262 197 180 144
SnaBI TAC'GTA
Cuts at: 493 2386 6885 493
Size: 1893 4499 2702
Fragments arranged by size:
4499 2702 1893
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9094
SphI G_CATG'C
Cuts at: 1132 5275 6452 1132
Size: 4143 1177 3774
Fragments arranged by size:
4143 3774 1177
SspI AAT'ATT
Cuts at: 5 52 2994 7018 5
Size: 47 2942 4024 2081
Fragments arranged by size:
4024 2942 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9094
StyI C'CwwG_G
Cuts at: 513 1065 2004 2784 2851 3217 3332 3585 5251
Size: 552 939 780 67 366 115 253 1666
Cuts at: 5251 5788 5842 6533 513
Size: 537 54 691 3074
Fragments arranged by size:
3074 1666 939 780 691 552 537 366
253 115 67 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2167 5251 5543 7121 7138 7291 7476
Size: 1255 587 3084 292 1578 17 153 185
Cuts at: 7476 7921 325
Size: 445 1498
Fragments arranged by size:
3084 1578 1498 1255 587 445 292 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2938 4286 7340
Size: 274 80 33 51 1443 961 1348 3054
Cuts at: 7340 9052 96
Size: 1712 138
Fragments arranged by size:
3054 1712 1443 1348 961 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2592 2624 5518 5550 5957 5989 9054 9086 2592
Size: 32 2894 32 407 32 3065 32 2600
Fragments arranged by size:
3065 2894 2600 407 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3781 4138 1316
Size: 2465 357 6272
Fragments arranged by size:
6272 2465 357
XbaI T'CTAG_A
Cuts at: 3740 4783 4970 6430 3740
Size: 1043 187 1460 6404
Fragments arranged by size:
6404 1460 1043 187
XhoI C'TCGA_G
Cuts at: 1943 2433 1943
Size: 490 8604
XmaI C'CCGG_G
Cuts at: 1870 5548 6419 1870
Size: 3678 871 4545
Fragments arranged by size:
4545 3678 871
XmnI GAAnn'nnTTC
Cuts at: 4521 7223 4521
Size: 2702 6392
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6902 276
Size: 53 83 186 936 5368 2468
Fragments arranged by size:
5368 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BfrBI BplI BsiWI BstZ17I
DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI RsrII SacI SfiI SfoI
SrfI SwaI XcmI