Restriction map for
pFN21AA0611
(Circular) (Six-base) MAPSORT of: pFN21AA0611.seq Check: 6492 from: 1 to: 7438
pFN21AA0611 7438 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3966 5248 278
Size: 53 83 186 936 2430 1282 2468
Fragments arranged by size:
2468 2430 1282 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2373 3078 4781 1937
Size: 436 705 1703 4594
Fragments arranged by size:
4594 1703 705 436
Acc65I G'GTAC_C
Cuts at: 2580 4759 2580
Size: 2179 5259
AclI AA'CG_TT
Cuts at: 1563 5565 5938 1563
Size: 4002 373 3063
Fragments arranged by size:
4002 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2543 3896 5501 6885 7153 1632
Size: 911 1353 1605 1384 268 1917
Fragments arranged by size:
1917 1605 1384 1353 911 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7241
AflIII A'CryG_T
Cuts at: 1203 6337 7314 1203
Size: 5134 977 1327
Fragments arranged by size:
5134 1327 977
AgeI A'CCGG_T
Cuts at: 6269 6269
Size: 7438
AhdI GACnn_n'nnGTC
Cuts at: 6167 6167
Size: 7438
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2978 3275 3786 1184
Size: 1794 297 511 4836
Fragments arranged by size:
4836 1794 511 297
AlwNI CAG_nnn'CTG
Cuts at: 3196 5181 6753 3196
Size: 1985 1572 3881
Fragments arranged by size:
3881 1985 1572
ApaI G_GGCC'C
Cuts at: 2921 2921
Size: 7438
ApaLI G'TGCA_C
Cuts at: 2259 2963 3470 3652 4448 5495 6651 2259
Size: 704 507 182 796 1047 1156 3046
Fragments arranged by size:
3046 1156 1047 796 704 507 182
ApoI r'AATT_y
Cuts at: 1493 4747 4973 5125 7234 1493
Size: 3254 226 152 2109 1697
Fragments arranged by size:
3254 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5992 160
Size: 872 4960 1606
Fragments arranged by size:
4960 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2702 3372 3737 3784 4763 1870
Size: 73 153 606 670 365 47 979 4545
Fragments arranged by size:
4545 979 670 606 365 153 73 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2388 2421 2388
Size: 33 7405
BamHI G'GATC_C
Cuts at: 4768 5135 4768
Size: 367 7071
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 2522 2580 4759 6214
Size: 325 217 627 304 431 58 2179 1455
Cuts at: 6214 618
Size: 1842
Fragments arranged by size:
2179 1842 1455 627 431 325 304 217
58
BanII G_rGCy'C
Cuts at: 1391 2921 4323 4757 1391
Size: 1530 1402 434 4072
Fragments arranged by size:
4072 1530 1402 434
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3069 3153 4705 928
Size: 971 1170 84 1552 3661
Fragments arranged by size:
3661 1552 1170 971 84
BbvCI CC'TCA_GC
Cuts at: 3042 3173 4121 3042
Size: 131 948 6359
Fragments arranged by size:
6359 948 131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5629 5663 1903
Size: 34 3692 34 3678
Fragments arranged by size:
3692 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2127 4323 5330 6540 2127
Size: 2196 1007 1210 3025
Fragments arranged by size:
3025 2196 1210 1007
BclI T'GATC_A
Cuts at: 1548 3034 1548
Size: 1486 5952
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2978 3543 6049
Size: 107 122 71 631 605 1306 565 2506
Cuts at: 6049 136
Size: 1525
Fragments arranged by size:
2506 1525 1306 631 605 565 122 107
71
BglII A'GATC_T
Cuts at: 3216 7433 3216
Size: 4217 3221
BlpI GC'TnA_GC
Cuts at: 4553 4855 4553
Size: 302 7136
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2263 2527 2921 2967 3474 3656 3775
Size: 304 169 264 394 46 507 182 119
Cuts at: 3775 3854 3880 4452 5499 6655 1790
Size: 79 26 572 1047 1156 2573
Fragments arranged by size:
2573 1156 1047 572 507 394 304 264
182 169 119 79 46 26
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7438
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2284 5169 6127
Size: 416 53 83 399 24 867 2885 958
Cuts at: 6127 442
Size: 1753
Fragments arranged by size:
2885 1753 958 867 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7438
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2136 2136
Size: 7438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2704 3297 3533 3605 3659 3818 6098 1239
Size: 1465 593 236 72 54 159 2280 2579
Fragments arranged by size:
2579 2280 1465 593 236 159 72 54
Bpu10I CC'TnA_GC
Cuts at: 2733 3042 3173 4121 4317 2733
Size: 309 131 948 196 5854
Fragments arranged by size:
5854 948 309 196 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3794 4322 4670 5563 6428 6726 3794
Size: 528 348 893 865 298 4506
Fragments arranged by size:
4506 893 865 528 348 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2100 2542 3147 3581 6101 882
Size: 617 601 442 605 434 2520 2219
Fragments arranged by size:
2520 2219 617 605 601 442 434
BsaAI yAC'GTr
Cuts at: 493 1204 2344 3651 5229 493
Size: 711 1140 1307 1578 2702
Fragments arranged by size:
2702 1578 1307 1140 711
BsaBI GATnn'nnATC
Cuts at: 4388 7432 4388
Size: 3044 4394
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3963 5245 5627 6262
Size: 53 83 186 936 2430 1282 382 635
Cuts at: 6262 275
Size: 1451
Fragments arranged by size:
2430 1451 1282 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2436 5871 6269 6543 6690 7101 2436
Size: 3435 398 274 147 411 2773
Fragments arranged by size:
3435 2773 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3050 3080 3294 3324 3341
Size: 30 165 30 1647 30 214 30 17
Cuts at: 3341 3371 3464 3494 4135 4165 4181 4211 4559
Size: 30 93 30 641 30 16 30 348
Cuts at: 4559 4589 1178
Size: 30 4027
Fragments arranged by size:
4027 1647 641 348 214 165 93 30
30 30 30 30 30 30 30 30
17 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2342 2594 3434 1186
Size: 1156 252 840 5190
Fragments arranged by size:
5190 1156 840 252
BseYI C'CCAG_C
Cuts at: 2086 2673 2740 2886 4715 6641 7137 2086
Size: 587 67 146 1829 1926 496 2387
Fragments arranged by size:
2387 1926 1829 587 496 146 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3350 3960 1163
Size: 573 1614 610 4641
Fragments arranged by size:
4641 1614 610 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3478 3858 4349 4888 5649 6677
Size: 275 186 1477 380 491 539 761 1028
Cuts at: 6677 1540
Size: 2301
Fragments arranged by size:
2301 1477 1028 761 539 491 380 275
186
BsiHKAI G_wGCw'C
Cuts at: 2263 2967 3393 3474 3656 4323 4406 4452 5499
Size: 704 426 81 182 667 83 46 1047
Cuts at: 5499 5584 6655 2263
Size: 85 1071 3046
Fragments arranged by size:
3046 1071 1047 704 667 426 182 85
83 81 46
BsmI GAATG_Cn'
Cuts at: 3604 3670 4949 5042 3604
Size: 66 1279 93 6000
Fragments arranged by size:
6000 1279 93 66
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2263 2527 2921 2967 3393 3474
Size: 399 304 169 264 394 46 426 81
Cuts at: 3474 3656 3775 3854 3880 4323 4406 4452 4757
Size: 182 119 79 26 443 83 46 305
Cuts at: 4757 5499 5584 6655 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 443 426 399 394 305
304 264 182 169 119 85 83 81
79 46 46 26
BspEI T'CCGG_A
Cuts at: 2436 7101 2436
Size: 4665 2773
BspHI T'CATG_A
Cuts at: 2445 4533 5327 7057 2445
Size: 2088 794 1730 2826
Fragments arranged by size:
2826 2088 1730 794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2592 4701 4793 844
Size: 1748 2109 92 3489
Fragments arranged by size:
3489 2109 1748 92
BsrBI CCG'CTC
Cuts at: 1394 1823 3480 5325 1394
Size: 429 1657 1845 3507
Fragments arranged by size:
3507 1845 1657 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3964 5933 6107 65
Size: 1183 2716 1969 174 1396
Fragments arranged by size:
2716 1969 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2379 2905 4071 6082 6269 1700
Size: 110 569 526 1166 2011 187 2869
Fragments arranged by size:
2869 2011 1166 569 526 187 110
BsrGI T'GTAC_A
Cuts at: 96 2667 96
Size: 2571 4867
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7438
BssSI C'ACGA_G
Cuts at: 2391 3392 4592 5498 6510 2391
Size: 1001 1200 906 1012 3319
Fragments arranged by size:
3319 1200 1012 1001 906
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7438
BstBI TT'CG_AA
Cuts at: 2683 6242 2683
Size: 3559 3879
BstEII G'GTnAC_C
Cuts at: 1184 3676 3712 1184
Size: 2492 36 4910
Fragments arranged by size:
4910 2492 36
BstYI r'GATC_y
Cuts at: 1972 2331 2511 3192 3216 4330 4768 5135 5520
Size: 359 180 681 24 1114 438 367 385
Cuts at: 5520 5537 6989 7075 7087 7433 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1114 681 438 385 367 359
346 180 86 24 17 12
BstZ17I GTA'TAC
Cuts at: 3079 3079
Size: 7438
Bsu36I CC'TnA_GG
Cuts at: 3593 3593
Size: 7438
BtgI C'CryG_G
Cuts at: 513 1065 2004 2709 3495 3721 513
Size: 552 939 705 786 226 4230
Fragments arranged by size:
4230 939 786 705 552 226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2968 4555 4600 487
Size: 683 56 1742 1587 45 3325
Fragments arranged by size:
3325 1742 1587 683 56 45
BtsI GCAGTG_nn'
Cuts at: 4954 5748 5768 4954
Size: 794 20 6624
Fragments arranged by size:
6624 794 20
ClaI AT'CG_AT
Cuts at: 1554 4602 1554
Size: 3048 4390
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7403
DraI TTT'AAA
Cuts at: 4742 4742
Size: 7438
DraIII CAC_nnn'GTG
Cuts at: 3001 4456 3001
Size: 1455 5983
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2357 4286 6445 809
Size: 507 1041 1929 2159 1802
Fragments arranged by size:
2159 1929 1802 1041 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2298 3492 3855
Size: 54 1425 50 275 18 468 1194 363
Cuts at: 3855 4069 4090 4346 4458 4885 5774 7403 8
Size: 214 21 256 112 427 889 1629 43
Fragments arranged by size:
1629 1425 1194 889 468 427 363 275
256 214 112 54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3855 4346 4885 1537
Size: 275 2043 491 539 4090
Fragments arranged by size:
4090 2043 539 491 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 3348 5368 1353
Size: 743 1252 2020 3423
Fragments arranged by size:
3423 2020 1252 743
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2959 4559 6025 6399 6545 387
Size: 1064 138 1370 1600 1466 374 146 1280
Fragments arranged by size:
1600 1466 1370 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4321 4321
Size: 7438
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2543 2704 3297 3533 3605 3659 3818
Size: 393 911 161 593 236 72 54 159
Cuts at: 3818 3896 5501 6098 6885 7153 1239
Size: 78 1605 597 787 268 1524
Fragments arranged by size:
1605 1524 911 787 597 593 393 268
236 161 159 78 72 54
EcoNI CCTnn'n_nnAGG
Cuts at: 3754 3911 3754
Size: 157 7281
EcoO109I rG'GnC_Cy
Cuts at: 2016 2070 2899 2917 3177 2016
Size: 54 829 18 260 6277
Fragments arranged by size:
6277 829 260 54 18
EcoRI G'AATT_C
Cuts at: 4747 5125 4747
Size: 378 7060
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2533 4384 2533
Size: 1851 5587
FspI TGC'GCA
Cuts at: 5944 5944
Size: 7438
HaeII r_GCGC'y
Cuts at: 1678 4424 4508 6585 1678
Size: 2746 84 2077 2531
Fragments arranged by size:
2746 2531 2077 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2764 2796 4209 4241 4277
Size: 32 135 32 1192 32 1413 32 36
Cuts at: 4277 4309 6079 6111 6153 6185 1373
Size: 32 1770 32 42 32 2626
Fragments arranged by size:
2626 1770 1413 1192 135 42 36 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2362 4782 5028 669
Size: 988 281 424 2420 246 3079
Fragments arranged by size:
3079 2420 988 424 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7438
HpaI GTT'AAC
Cuts at: 5028 5028
Size: 7438
KpnI G_GTAC'C
Cuts at: 2584 4763 2584
Size: 2179 5259
MfeI C'AATT_G
Cuts at: 5037 5037
Size: 7438
MluI A'CGCG_T
Cuts at: 7314 7314
Size: 7438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2640 3045 3327 4419 6552 6736 1305
Size: 1335 405 282 1092 2133 184 2007
Fragments arranged by size:
2133 2007 1335 1092 405 282 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3494 4460 10
Size: 54 1425 343 1662 966 2988
Fragments arranged by size:
2988 1662 1425 966 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3257 3768 4409 4538 4604 4633 5396
Size: 648 2091 511 641 129 66 29 763
Cuts at: 5396 5755 5914 518
Size: 359 159 2042
Fragments arranged by size:
2091 2042 763 648 641 511 359 159
129 66 29
MspA1I CmG'CkG
Cuts at: 1606 2673 2740 3172 3559 3723 4665 4854 5531
Size: 1067 67 432 387 164 942 189 677
Cuts at: 5531 6679 6924 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1067 942 677 432 387 245
189 164 67
NaeI GCC'GGC
Cuts at: 2381 2381
Size: 7438
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5947
Fragments arranged by size:
5947 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7438
NgoMIV G'CCGG_C
Cuts at: 2379 2379
Size: 7438
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7438
NotI GC'GGCC_GC
Cuts at: 4885 4885
Size: 7438
NspI r_CATG'y
Cuts at: 1132 1229 4796 6341 1132
Size: 97 3567 1545 2229
Fragments arranged by size:
3567 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2284 1348
Size: 936 6502
PciI A'CATG_T
Cuts at: 6337 6337
Size: 7438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7438
PfoI T'CCnGG_A
Cuts at: 4275 4275
Size: 7438
PmeI GTTT'AAAC
Cuts at: 4742 4742
Size: 7438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 4427 4459 5507 5539 7020 7052 3812
Size: 32 583 32 1048 32 1481 32 4198
Fragments arranged by size:
4198 1481 1048 583 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2899 3177 2899
Size: 278 7160
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7438
PsiI TTA'TAA
Cuts at: 5008 5008
Size: 7438
PspOMI G'GGCC_C
Cuts at: 2917 2917
Size: 7438
PspXI vC'TCGA_Gb
Cuts at: 3737 3737
Size: 7438
PstI C_TGCA'G
Cuts at: 830 2589 3564 4039 4790 830
Size: 1759 975 475 751 3478
Fragments arranged by size:
3478 1759 975 751 475
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7438
PvuII CAG'CTG
Cuts at: 2673 2740 3172 3559 4665 2673
Size: 67 432 387 1106 5446
Fragments arranged by size:
5446 1106 432 387 67
SacI G_AGCT'C
Cuts at: 4323 4323
Size: 7438
SacII CC_GC'GG
Cuts at: 3724 3724
Size: 7438
SalI G'TCGA_C
Cuts at: 1936 4780 1936
Size: 2844 4594
SbfI CC_TGCA'GG
Cuts at: 4790 4790
Size: 7438
ScaI AGT'ACT
Cuts at: 3815 5686 7398 3815
Size: 1871 1712 3855
Fragments arranged by size:
3855 1871 1712
SfcI C'TryA_G
Cuts at: 826 1045 2374 2585 3212 3560 4035 4786 5921
Size: 219 1329 211 627 348 475 751 1135
Cuts at: 5921 6602 6793 826
Size: 681 191 1471
Fragments arranged by size:
1471 1329 1135 751 681 627 475 348
219 211 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7438
SmaI CCC'GGG
Cuts at: 1872 2704 4765 1872
Size: 832 2061 4545
Fragments arranged by size:
4545 2061 832
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3737 3809 4337 4685 5542 6443
Size: 197 926 1794 72 528 348 857 901
Cuts at: 6443 6705 820
Size: 262 1553
Fragments arranged by size:
1794 1553 926 901 857 528 348 262
197 72
SnaBI TAC'GTA
Cuts at: 493 5229 493
Size: 4736 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7438
SphI G_CATG'C
Cuts at: 1132 4796 1132
Size: 3664 3774
SspI AAT'ATT
Cuts at: 5 52 5362 5
Size: 47 5310 2081
Fragments arranged by size:
5310 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7438
StyI C'CwwG_G
Cuts at: 513 1065 2004 4877 513
Size: 552 939 2873 3074
Fragments arranged by size:
3074 2873 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2396 2665 2900 3899 5465 5482 5635
Size: 1255 816 269 235 999 1566 17 153
Cuts at: 5635 5820 6265 325
Size: 185 445 1498
Fragments arranged by size:
1566 1498 1255 999 816 445 269 235
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 2667 2857
Size: 274 80 33 51 1443 168 522 190
Cuts at: 2857 3333 3813 4608 5684 7396 96
Size: 476 480 795 1076 1712 138
Fragments arranged by size:
1712 1443 1076 795 522 480 476 274
190 168 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2149 2181 3112 3144 3294 3326 3341 3373 3464
Size: 32 931 32 150 32 15 32 91
Cuts at: 3464 3496 3518 3550 4240 4272 7398 7430 2149
Size: 32 22 32 690 32 3126 32 2157
Fragments arranged by size:
3126 2157 931 690 150 91 32 32
32 32 32 32 32 32 22 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7438
XbaI T'CTAG_A
Cuts at: 4774 4774
Size: 7438
XhoI C'TCGA_G
Cuts at: 1943 3737 1943
Size: 1794 5644
XmaI C'CCGG_G
Cuts at: 1870 2702 4763 1870
Size: 832 2061 4545
Fragments arranged by size:
4545 2061 832
XmnI GAAnn'nnTTC
Cuts at: 3749 5567 3749
Size: 1818 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3964 5246 276
Size: 53 83 186 936 2430 1282 2468
Fragments arranged by size:
2468 2430 1282 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstXI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PsrI RsrII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI