Restriction map for
pFN21AA0609
(Circular) (Six-base) MAPSORT of: pFN21AA0609.seq Check: 5387 from: 1 to: 6817
pFN21AA0609 6817 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3270 4627 278
Size: 53 83 186 936 1734 1357 2468
Fragments arranged by size:
2468 1734 1357 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4160 1937
Size: 2223 4594
Acc65I G'GTAC_C
Cuts at: 2832 3726 4138 2832
Size: 894 412 5511
Fragments arranged by size:
5511 894 412
AclI AA'CG_TT
Cuts at: 1563 4944 5317 1563
Size: 3381 373 3063
Fragments arranged by size:
3381 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2643 4880 6264 6532 1632
Size: 1011 2237 1384 268 1917
Fragments arranged by size:
2237 1917 1384 1011 268
AfeI AGC'GCT
Cuts at: 3197 3197
Size: 6817
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6620
AflIII A'CryG_T
Cuts at: 1203 3120 3522 4059 5716 6693 1203
Size: 1917 402 537 1657 977 1327
Fragments arranged by size:
1917 1657 1327 977 537 402
AgeI A'CCGG_T
Cuts at: 5648 5648
Size: 6817
AhdI GACnn_n'nnGTC
Cuts at: 5546 5546
Size: 6817
AleI CACnn'nnGTG
Cuts at: 1166 2897 1166
Size: 1731 5086
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6817
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3449 3481 3914 3946 3449
Size: 32 433 32 6320
Fragments arranged by size:
6320 433 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2548 3310 4560 6132 2548
Size: 762 1250 1572 3233
Fragments arranged by size:
3233 1572 1250 762
ApaI G_GGCC'C
Cuts at: 3342 3342
Size: 6817
ApaLI G'TGCA_C
Cuts at: 4874 6030 4874
Size: 1156 5661
ApoI r'AATT_y
Cuts at: 1493 2033 4126 4352 4504 6613 1493
Size: 540 2093 226 152 2109 1697
Fragments arranged by size:
2109 2093 1697 540 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5371 160
Size: 872 4339 1606
Fragments arranged by size:
4339 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6817
AvaI C'yCGr_G
Cuts at: 1870 1943 4142 1870
Size: 73 2199 4545
Fragments arranged by size:
4545 2199 73
BamHI G'GATC_C
Cuts at: 2634 4147 4514 2634
Size: 1513 367 4937
Fragments arranged by size:
4937 1513 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2575 2832 3726 4138 5593
Size: 325 217 627 788 257 894 412 1455
Cuts at: 5593 618
Size: 1842
Fragments arranged by size:
1842 1455 894 788 627 412 325 257
217
BanII G_rGCy'C
Cuts at: 1391 2275 2616 2976 3342 3407 3564 4136 1391
Size: 884 341 360 366 65 157 572 4072
Fragments arranged by size:
4072 884 572 366 360 341 157 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2048 928
Size: 971 149 5697
Fragments arranged by size:
5697 971 149
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5008 5042 1903
Size: 34 50 34 2987 34 3678
Fragments arranged by size:
3678 2987 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2435 4709 5919 2435
Size: 2274 1210 3333
Fragments arranged by size:
3333 2274 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6817
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4074 5428 136
Size: 107 122 71 631 605 2402 1354 1525
Fragments arranged by size:
2402 1525 1354 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6812 6812
Size: 6817
BlpI GC'TnA_GC
Cuts at: 2252 3282 4234 2252
Size: 1030 952 4835
Fragments arranged by size:
4835 1030 952
Bme1580I G_kGCm'C
Cuts at: 1790 2327 2580 3342 4878 6034 1790
Size: 537 253 762 1536 1156 2573
Fragments arranged by size:
2573 1536 1156 762 537 253
BmgBI CAC'GTC
Cuts at: 1318 3121 3733 3842 1318
Size: 1803 612 109 4293
Fragments arranged by size:
4293 1803 612 109
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3340 4548 5506
Size: 416 53 83 399 24 1923 1208 958
Cuts at: 5506 442
Size: 1753
Fragments arranged by size:
1923 1753 1208 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6817
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2158 2159 2219 2349 2729 3122 3287 3332
Size: 919 1 60 130 380 393 165 45
Cuts at: 3332 5477 1239
Size: 2145 2579
Fragments arranged by size:
2579 2145 919 393 380 165 130 60
45 1
Bpu10I CC'TnA_GC
Cuts at: 2998 2998
Size: 6817
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2600 4022 4942 5807 6105 2600
Size: 1422 920 865 298 3312
Fragments arranged by size:
3312 1422 920 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5480 882
Size: 617 3981 2219
Fragments arranged by size:
3981 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4608 493
Size: 711 3404 2702
Fragments arranged by size:
3404 2702 711
BsaBI GATnn'nnATC
Cuts at: 6811 6811
Size: 6817
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3267 3369 4624 5006
Size: 53 83 186 936 1734 102 1255 382
Cuts at: 5006 5641 275
Size: 635 1451
Fragments arranged by size:
1734 1451 1255 936 635 382 186 102
83 53
BsaWI w'CCGG_w
Cuts at: 3861 5250 5648 5922 6069 6480 3861
Size: 1389 398 274 147 411 4198
Fragments arranged by size:
4198 1389 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2156 2186 2933 2963 3083
Size: 30 165 30 753 30 747 30 120
Cuts at: 3083 3113 3449 3479 3674 3704 3914 3944 1178
Size: 30 336 30 195 30 210 30 4051
Fragments arranged by size:
4051 753 747 336 210 195 165 120
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 2240 2333 1186
Size: 1051 3 93 5670
Fragments arranged by size:
5670 1051 93 3
BseYI C'CCAG_C
Cuts at: 2008 2068 3378 3977 6020 6516 2008
Size: 60 1310 599 2043 496 2309
Fragments arranged by size:
2309 2043 1310 599 496 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2492 3098 3215 3938 1163
Size: 573 756 606 117 723 4042
Fragments arranged by size:
4042 756 723 606 573 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2397 3862 4267 5028 6056 1540
Size: 275 186 396 1465 405 761 1028 2301
Fragments arranged by size:
2301 1465 1028 761 405 396 275 186
BsiHKAI G_wGCw'C
Cuts at: 2616 2976 4878 4963 6034 2616
Size: 360 1902 85 1071 3399
Fragments arranged by size:
3399 1902 1071 360 85
BsmI GAATG_Cn'
Cuts at: 4328 4421 4328
Size: 93 6724
BsmBI CGTCTCn'nnnn_
Cuts at: 2020 3111 3832 2020
Size: 1091 721 5005
Fragments arranged by size:
5005 1091 721
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2275 2327 2580 2616 2976 3342 3407
Size: 399 485 52 253 36 360 366 65
Cuts at: 3407 3564 4136 4878 4963 6034 1391
Size: 157 572 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 572 485 399 366 360
253 157 85 65 52 36
BspEI T'CCGG_A
Cuts at: 6480 6480
Size: 6817
BspHI T'CATG_A
Cuts at: 4706 6436 4706
Size: 1730 5087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3489 4172 844
Size: 2645 683 3489
Fragments arranged by size:
3489 2645 683
BsrBI CCG'CTC
Cuts at: 1394 1823 3099 4704 1394
Size: 429 1276 1605 3507
Fragments arranged by size:
3507 1605 1276 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2532 2754 3627 5312 5486 65
Size: 1183 1284 222 873 1685 174 1396
Fragments arranged by size:
1685 1396 1284 1183 873 222 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2392 3654 5461 5648 1700
Size: 110 582 1262 1807 187 2869
Fragments arranged by size:
2869 1807 1262 582 187 110
BsrGI T'GTAC_A
Cuts at: 96 3450 96
Size: 3354 3463
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6817
BssSI C'ACGA_G
Cuts at: 4877 5889 4877
Size: 1012 5805
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6817
BstBI TT'CG_AA
Cuts at: 2106 5621 2106
Size: 3515 3302
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6817
BstXI CCAn_nnnn'nTGG
Cuts at: 3349 3349
Size: 6817
BstYI r'GATC_y
Cuts at: 1972 2544 2634 4147 4514 4899 4916 6368 6454
Size: 572 90 1513 367 385 17 1452 86
Cuts at: 6454 6466 6812 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1513 1452 572 385 367 346 90
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3575 3575
Size: 6817
BtgI C'CryG_G
Cuts at: 513 1065 2313 2864 3291 513
Size: 552 1248 551 427 4039
Fragments arranged by size:
4039 1248 552 551 427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2267 487
Size: 683 56 1041 5037
Fragments arranged by size:
5037 1041 683 56
BtsI GCAGTG_nn'
Cuts at: 3113 3644 4333 5127 5147 3113
Size: 531 689 794 20 4783
Fragments arranged by size:
4783 794 689 531 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6817
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2737 2772 529
Size: 35 2173 35 4574
Fragments arranged by size:
4574 2173 35 35
DraI TTT'AAA
Cuts at: 4121 4121
Size: 6817
DraIII CAC_nnn'GTG
Cuts at: 3433 3448 3433
Size: 15 6802
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5824 809
Size: 507 4508 1802
Fragments arranged by size:
4508 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2550 2867 3495
Size: 54 1425 50 275 18 720 317 628
Cuts at: 3495 4264 5153 6782 8
Size: 769 889 1629 43
Fragments arranged by size:
1629 1425 889 769 720 628 317 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4264 1537
Size: 275 2452 4090
Fragments arranged by size:
4090 2452 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2564 2700 2875 3719 4038 4747 1353
Size: 1211 136 175 844 319 709 3423
Fragments arranged by size:
3423 1211 844 709 319 175 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3790 5404 5778 5924 387
Size: 1064 138 2201 1614 374 146 1280
Fragments arranged by size:
2201 1614 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2614 2974 2614
Size: 360 6457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2158 2159 2219 2349 2643 2729 3122
Size: 393 526 1 60 130 294 86 393
Cuts at: 3122 3287 3332 4880 5477 6264 6532 1239
Size: 165 45 1548 597 787 268 1524
Fragments arranged by size:
1548 1524 787 597 526 393 393 294
268 165 130 86 60 45 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2261 2261
Size: 6817
EcoO109I rG'GnC_Cy
Cuts at: 3338 3708 3338
Size: 370 6447
EcoRI G'AATT_C
Cuts at: 4126 4504 4126
Size: 378 6439
EcoRV GAT'ATC
Cuts at: 2737 2737
Size: 6817
FspI TGC'GCA
Cuts at: 2207 3488 5323 2207
Size: 1281 1835 3701
Fragments arranged by size:
3701 1835 1281
FspAI rTGC'GCAy
Cuts at: 2207 2207
Size: 6817
HaeII r_GCGC'y
Cuts at: 1678 3199 5964 1678
Size: 1521 2765 2531
Fragments arranged by size:
2765 2531 1521
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2603 2635 2650 2677 2682
Size: 32 135 32 1031 32 15 27 5
Cuts at: 2682 2706 2709 2738 3674 3706 5458 5490 5532
Size: 24 3 29 936 32 1752 32 42
Cuts at: 5532 5564 1373
Size: 32 2626
Fragments arranged by size:
2626 1752 1031 936 135 42 32 32
32 32 32 32 29 27 24 15
5 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4161 4407 669
Size: 988 281 2223 246 3079
Fragments arranged by size:
3079 2223 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2675 748
Size: 1927 4890
HpaI GTT'AAC
Cuts at: 4407 4407
Size: 6817
KpnI G_GTAC'C
Cuts at: 2836 3730 4142 2836
Size: 894 412 5511
Fragments arranged by size:
5511 894 412
MfeI C'AATT_G
Cuts at: 4416 4416
Size: 6817
MluI A'CGCG_T
Cuts at: 6693 6693
Size: 6817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4029 5931 6115 1305
Size: 2724 1902 184 2007
Fragments arranged by size:
2724 2007 1902 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2552 2869 3497 10
Size: 54 1425 343 720 317 628 3330
Fragments arranged by size:
3330 1425 720 628 343 317 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2897 2981 4775 5134 5293 518
Size: 648 1731 84 1794 359 159 2042
Fragments arranged by size:
2042 1794 1731 648 359 159 84
MspA1I CmG'CkG
Cuts at: 1606 2137 2182 2582 3082 3148 3286 4233 4910
Size: 531 45 400 500 66 138 947 677
Cuts at: 4910 6058 6303 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 947 677 531 500 400 245
138 66 45
NcoI C'CATG_G
Cuts at: 513 1065 2313 2864 3291 513
Size: 552 1248 551 427 4039
Fragments arranged by size:
4039 1248 552 551 427
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6817
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6817
NotI GC'GGCC_GC
Cuts at: 4264 4264
Size: 6817
NspI r_CATG'y
Cuts at: 1132 1229 2212 2986 3526 3967 4063 4175 5720
Size: 97 983 774 540 441 96 112 1545
Cuts at: 5720 1132
Size: 2229
Fragments arranged by size:
2229 1545 983 774 540 441 112 97
96
PasI CC'CwG_GG
Cuts at: 1348 2263 1348
Size: 915 5902
PciI A'CATG_T
Cuts at: 3522 4059 5716 3522
Size: 537 1657 4623
Fragments arranged by size:
4623 1657 537
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2079 2876 3310 3433 1108
Size: 971 797 434 123 4492
Fragments arranged by size:
4492 971 797 434 123
PfoI T'CCnGG_A
Cuts at: 2630 2630
Size: 6817
PmeI GTTT'AAAC
Cuts at: 4121 4121
Size: 6817
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 3914 3946 4886 4918 6399 6431 3449
Size: 32 433 32 940 32 1481 32 3835
Fragments arranged by size:
3835 1481 940 433 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3708 3708
Size: 6817
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6817
PsiI TTA'TAA
Cuts at: 4387 4387
Size: 6817
PspOMI G'GGCC_C
Cuts at: 3338 3338
Size: 6817
PstI C_TGCA'G
Cuts at: 830 2021 3253 4169 830
Size: 1191 1232 916 3478
Fragments arranged by size:
3478 1232 1191 916
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6817
PvuII CAG'CTG
Cuts at: 3082 3148 3286 3082
Size: 66 138 6613
Fragments arranged by size:
6613 138 66
RsrII CG'GwC_CG
Cuts at: 3777 3777
Size: 6817
SacI G_AGCT'C
Cuts at: 2616 2976 2616
Size: 360 6457
SalI G'TCGA_C
Cuts at: 1936 4159 1936
Size: 2223 4594
SanDI GG'GwC_CC
Cuts at: 3708 3708
Size: 6817
SapI GCTCTTCn'nnn_
Cuts at: 2564 2564
Size: 6817
SbfI CC_TGCA'GG
Cuts at: 4169 4169
Size: 6817
ScaI AGT'ACT
Cuts at: 5065 6777 5065
Size: 1712 5105
SfcI C'TryA_G
Cuts at: 826 1045 2017 3112 3249 4165 5300 5981 6172
Size: 219 972 1095 137 916 1135 681 191
Cuts at: 6172 826
Size: 1471
Fragments arranged by size:
1471 1135 1095 972 916 681 219 191
137
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6817
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6817
SmaI CCC'GGG
Cuts at: 1872 4144 1872
Size: 2272 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2615 4037 4921 5822 6084 820
Size: 197 926 672 1422 884 901 262 1553
Fragments arranged by size:
1553 1422 926 901 884 672 262 197
SnaBI TAC'GTA
Cuts at: 493 4608 493
Size: 4115 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6817
SphI G_CATG'C
Cuts at: 1132 2212 2986 4175 1132
Size: 1080 774 1189 3774
Fragments arranged by size:
3774 1189 1080 774
SspI AAT'ATT
Cuts at: 5 52 4741 5
Size: 47 4689 2081
Fragments arranged by size:
4689 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6817
StyI C'CwwG_G
Cuts at: 513 1065 2313 2836 2864 2875 3291 4256 513
Size: 552 1248 523 28 11 416 965 3074
Fragments arranged by size:
3074 1248 965 552 523 416 28 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3230 3769 4844 4861 5014 5199 5644
Size: 1255 1650 539 1075 17 153 185 445
Cuts at: 5644 325
Size: 1498
Fragments arranged by size:
1650 1498 1255 1075 539 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3222 3450 5063
Size: 274 80 33 51 1443 1245 228 1613
Cuts at: 5063 6775 96
Size: 1712 138
Fragments arranged by size:
1712 1613 1443 1245 274 228 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2106 2138 2933 2965 3083 3115 3158 3190 6777
Size: 32 795 32 118 32 43 32 3587
Cuts at: 6777 6809 2106
Size: 32 2114
Fragments arranged by size:
3587 2114 795 118 43 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6817
XbaI T'CTAG_A
Cuts at: 4153 4153
Size: 6817
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2076 2076
Size: 6817
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6817
XmaI C'CCGG_G
Cuts at: 1870 4142 1870
Size: 2272 4545
XmnI GAAnn'nnTTC
Cuts at: 4047 4946 4047
Size: 899 5918
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3268 4625 276
Size: 53 83 186 936 1734 1357 2468
Fragments arranged by size:
2468 1734 1357 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BstZ17I FalI FseI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI SacII SexAI
SfiI SfoI SrfI SwaI