Restriction map for
pFN21AA0606
(Circular) (Six-base) MAPSORT of: pFN21AA0606.seq Check: 8918 from: 1 to: 8320
pFN21AA0606 8320 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5223 5223
Size: 8320
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6130 278
Size: 53 83 186 936 4594 2468
Fragments arranged by size:
4594 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5663 1937
Size: 3726 4594
Acc65I G'GTAC_C
Cuts at: 5641 5641
Size: 8320
AclI AA'CG_TT
Cuts at: 1563 6447 6820 1563
Size: 4884 373 3063
Fragments arranged by size:
4884 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3596 4026 4179 4436 5204 6383 7767 8035
Size: 1964 430 153 257 768 1179 1384 268
Cuts at: 8035 1632
Size: 1917
Fragments arranged by size:
1964 1917 1384 1179 768 430 268 257
153
AfeI AGC'GCT
Cuts at: 3096 4717 5012 3096
Size: 1621 295 6404
Fragments arranged by size:
6404 1621 295
AflII C'TTAA_G
Cuts at: 820 1017 2341 820
Size: 197 1324 6799
Fragments arranged by size:
6799 1324 197
AflIII A'CryG_T
Cuts at: 1203 2628 2899 7219 8196 1203
Size: 1425 271 4320 977 1327
Fragments arranged by size:
4320 1425 1327 977 271
AgeI A'CCGG_T
Cuts at: 7151 7151
Size: 8320
AhdI GACnn_n'nnGTC
Cuts at: 2150 4606 7049 2150
Size: 2456 2443 3421
Fragments arranged by size:
3421 2456 2443
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8320
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2660 3737 1184
Size: 1476 1077 5767
Fragments arranged by size:
5767 1476 1077
AlwNI CAG_nnn'CTG
Cuts at: 4755 5215 6063 7635 4755
Size: 460 848 1572 5440
Fragments arranged by size:
5440 1572 848 460
ApaLI G'TGCA_C
Cuts at: 4135 6377 7533 4135
Size: 2242 1156 4922
Fragments arranged by size:
4922 2242 1156
ApoI r'AATT_y
Cuts at: 1493 2780 5629 5855 6007 8116 1493
Size: 1287 2849 226 152 2109 1697
Fragments arranged by size:
2849 2109 1697 1287 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6874 160
Size: 872 5842 1606
Fragments arranged by size:
5842 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8320
AvaI C'yCGr_G
Cuts at: 1870 1943 5645 1870
Size: 73 3702 4545
Fragments arranged by size:
4545 3702 73
AvrII C'CTAG_G
Cuts at: 4579 4579
Size: 8320
BamHI G'GATC_C
Cuts at: 4294 4304 5443 5650 6017 4294
Size: 10 1139 207 367 6597
Fragments arranged by size:
6597 1139 367 207 10
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4117 4256 4522 5224 5325
Size: 325 217 627 2330 139 266 702 101
Cuts at: 5325 5641 7096 618
Size: 316 1455 1842
Fragments arranged by size:
2330 1842 1455 702 627 325 316 266
217 139 101
BanII G_rGCy'C
Cuts at: 1391 3399 3979 4770 4911 4962 4975 5124 5639
Size: 2008 580 791 141 51 13 149 515
Cuts at: 5639 1391
Size: 4072
Fragments arranged by size:
4072 2008 791 580 515 149 141 51
13
BbeI G_GCGC'C
Cuts at: 5329 5329
Size: 8320
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5128 928
Size: 971 3229 4120
Fragments arranged by size:
4120 3229 971
BbvCI CC'TCA_GC
Cuts at: 5550 5550
Size: 8320
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4029 4063 6511 6545 1903
Size: 34 2092 34 2448 34 3678
Fragments arranged by size:
3678 2448 2092 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6212 7422 6212
Size: 1210 7110
BclI T'GATC_A
Cuts at: 1548 3898 3946 4833 5452 1548
Size: 2350 48 887 619 4416
Fragments arranged by size:
4416 2350 887 619 48
BfrBI ATG'CAT
Cuts at: 2215 2215
Size: 8320
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3340 4781 6931
Size: 107 122 71 631 605 1668 1441 2150
Cuts at: 6931 136
Size: 1525
Fragments arranged by size:
2150 1668 1525 1441 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4385 8315 4385
Size: 3930 4390
BlpI GC'TnA_GC
Cuts at: 4769 5436 5737 4769
Size: 667 301 7352
Fragments arranged by size:
7352 667 301
Bme1580I G_kGCm'C
Cuts at: 1790 3558 4122 4139 4647 6381 7537 1790
Size: 1768 564 17 508 1734 1156 2573
Fragments arranged by size:
2573 1768 1734 1156 564 508 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8320
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3134 4509 4791
Size: 416 53 83 399 24 1717 1375 282
Cuts at: 4791 4995 6051 7009 442
Size: 204 1056 958 1753
Fragments arranged by size:
1753 1717 1375 1056 958 416 399 282
204 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3459 3665 4115 4858 5433 5540 6980 1239
Size: 2220 206 450 743 575 107 1440 2579
Fragments arranged by size:
2579 2220 1440 743 575 450 206 107
Bpu10I CC'TnA_GC
Cuts at: 3803 4976 5550 3803
Size: 1173 574 6573
Fragments arranged by size:
6573 1173 574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3413 6445 7310 7608 3413
Size: 3032 865 298 4125
Fragments arranged by size:
4125 3032 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2849 4095 6983 882
Size: 617 1350 1246 2888 2219
Fragments arranged by size:
2888 2219 1350 1246 617
BsaAI yAC'GTr
Cuts at: 493 1204 4537 6111 493
Size: 711 3333 1574 2702
Fragments arranged by size:
3333 2702 1574 711
BsaBI GATnn'nnATC
Cuts at: 8314 8314
Size: 8320
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2146 5133 5326 6127
Size: 53 83 186 936 613 2987 193 801
Cuts at: 6127 6509 7144 275
Size: 382 635 1451
Fragments arranged by size:
2987 1451 936 801 635 613 382 193
186 83 53
BsaWI w'CCGG_w
Cuts at: 2204 3294 3971 4104 6753 7151 7425 7572 7983
Size: 1090 677 133 2649 398 274 147 411
Cuts at: 7983 2204
Size: 2541
Fragments arranged by size:
2649 2541 1090 677 411 398 274 147
133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3982 4012 1178
Size: 30 165 30 2579 30 5486
Fragments arranged by size:
5486 2579 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2275 4631 5132 5498 1186
Size: 1089 2356 501 366 4008
Fragments arranged by size:
4008 2356 1089 501 366
BseYI C'CCAG_C
Cuts at: 2376 3979 4238 4858 7523 8019 2376
Size: 1603 259 620 2665 496 2677
Fragments arranged by size:
2677 2665 1603 620 496 259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4559 4748 1163
Size: 573 2823 189 4735
Fragments arranged by size:
4735 2823 573 189
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5770 6531 7559 1540
Size: 275 186 3769 761 1028 2301
Fragments arranged by size:
3769 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3399 4139 4551 4770 4911 6381 6466 7537 3399
Size: 740 412 219 141 1470 85 1071 4182
Fragments arranged by size:
4182 1470 1071 740 412 219 141 85
BsmI GAATG_Cn'
Cuts at: 5831 5924 5831
Size: 93 8227
BsmBI CGTCTCn'nnnn_
Cuts at: 3957 3957
Size: 8320
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3399 3558 3979 4122 4139 4551 4647
Size: 399 1609 159 421 143 17 412 96
Cuts at: 4647 4770 4911 4962 4975 5124 5639 6381 6466
Size: 123 141 51 13 149 515 742 85
Cuts at: 6466 7537 1391
Size: 1071 2174
Fragments arranged by size:
2174 1609 1071 742 515 421 412 399
159 149 143 141 123 96 85 51
17 13
BspEI T'CCGG_A
Cuts at: 3294 3971 7983 3294
Size: 677 4012 3631
Fragments arranged by size:
4012 3631 677
BspHI T'CATG_A
Cuts at: 3705 4395 5499 6209 7939 3705
Size: 690 1104 710 1730 4086
Fragments arranged by size:
4086 1730 1104 710 690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2177 3852 5223 5675 844
Size: 1333 1675 1371 452 3489
Fragments arranged by size:
3489 1675 1371 1333 452
BsrBI CCG'CTC
Cuts at: 1394 1823 6207 1394
Size: 429 4384 3507
Fragments arranged by size:
4384 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2644 6815 6989 65
Size: 1183 1396 4171 174 1396
Fragments arranged by size:
4171 1396 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4775 6964 7151 1700
Size: 110 2965 2189 187 2869
Fragments arranged by size:
2965 2869 2189 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8320
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8320
BssSI C'ACGA_G
Cuts at: 2401 6380 7392 2401
Size: 3979 1012 3329
Fragments arranged by size:
3979 3329 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2221 4546 5215 794
Size: 1427 2325 669 3899
Fragments arranged by size:
3899 2325 1427 669
BstBI TT'CG_AA
Cuts at: 5404 7124 5404
Size: 1720 6600
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8320
BstXI CCAn_nnnn'nTGG
Cuts at: 3987 3987
Size: 8320
BstYI r'GATC_y
Cuts at: 1972 2905 3585 4294 4304 4385 5443 5650 6017
Size: 933 680 709 10 81 1058 207 367
Cuts at: 6017 6402 6419 7871 7957 7969 8315 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1058 933 709 680 385 367
346 207 86 81 17 12 10
Bsu36I CC'TnA_GG
Cuts at: 2483 2861 2483
Size: 378 7942
BtgI C'CryG_G
Cuts at: 513 1065 2004 4623 5570 513
Size: 552 939 2619 947 3263
Fragments arranged by size:
3263 2619 947 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7581
Fragments arranged by size:
7581 683 56
BtsI GCAGTG_nn'
Cuts at: 4909 5176 5836 6630 6650 4909
Size: 267 660 794 20 6579
Fragments arranged by size:
6579 794 660 267 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8285
DraI TTT'AAA
Cuts at: 2469 5624 2469
Size: 3155 5165
DraIII CAC_nnn'GTG
Cuts at: 3730 5374 3730
Size: 1644 6676
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7327 809
Size: 507 6011 1802
Fragments arranged by size:
6011 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3241 5767 6656
Size: 54 1425 50 275 18 1411 2526 889
Cuts at: 6656 8285 8
Size: 1629 43
Fragments arranged by size:
2526 1629 1425 1411 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5767 1537
Size: 275 3955 4090
Fragments arranged by size:
4090 3955 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2441 2543 3112 3486 4155 4404 4412 5406
Size: 1088 102 569 374 669 249 8 994
Cuts at: 5406 5475 6250 1353
Size: 69 775 3423
Fragments arranged by size:
3423 1088 994 775 669 569 374 249
102 69 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6907 7281 7427 387
Size: 1064 138 5318 374 146 1280
Fragments arranged by size:
5318 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3397 4768 4909 3397
Size: 1371 141 6808
Fragments arranged by size:
6808 1371 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3459 3596 3665 4026 4115 4179 4436
Size: 393 1827 137 69 361 89 64 257
Cuts at: 4436 4858 5204 5433 5540 6383 6980 7767 8035
Size: 422 346 229 107 843 597 787 268
Cuts at: 8035 1239
Size: 1524
Fragments arranged by size:
1827 1524 843 787 597 422 393 361
346 268 257 229 137 107 89 69
64
EcoNI CCTnn'n_nnAGG
Cuts at: 3957 5219 3957
Size: 1262 7058
EcoO109I rG'GnC_Cy
Cuts at: 2166 2166
Size: 8320
EcoRI G'AATT_C
Cuts at: 5629 6007 5629
Size: 378 7942
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2322 5317 2322
Size: 2995 5325
FspI TGC'GCA
Cuts at: 4637 6826 4637
Size: 2189 6131
HaeII r_GCGC'y
Cuts at: 1678 3098 4719 4776 5014 5056 5070 5085 5265
Size: 1420 1621 57 238 42 14 15 180
Cuts at: 5265 5329 7467 1678
Size: 64 2138 2531
Fragments arranged by size:
2531 2138 1621 1420 238 180 64 57
42 15 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2136 2168 4377 4409 4592
Size: 32 135 32 564 32 2209 32 183
Cuts at: 4592 4624 4825 4832 4857 4864 4925 4957 6961
Size: 32 201 7 25 7 61 32 2004
Cuts at: 6961 6993 7035 7067 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2209 2004 564 201 183 135 61
42 32 32 32 32 32 32 32
32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3564 4944 5664 5910 669
Size: 988 281 1626 1380 720 246 3079
Fragments arranged by size:
3079 1626 1380 988 720 281 246
HindIII A'AGCT_T
Cuts at: 748 2993 4008 4247 748
Size: 2245 1015 239 4821
Fragments arranged by size:
4821 2245 1015 239
HpaI GTT'AAC
Cuts at: 3564 5910 3564
Size: 2346 5974
KasI G'GCGC_C
Cuts at: 5325 5325
Size: 8320
KpnI G_GTAC'C
Cuts at: 5645 5645
Size: 8320
MfeI C'AATT_G
Cuts at: 5919 5919
Size: 8320
MluI A'CGCG_T
Cuts at: 8196 8196
Size: 8320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2633 2647 2679 2715 3768 5064 5115 7434
Size: 1328 14 32 36 1053 1296 51 2319
Cuts at: 7434 7618 1305
Size: 184 2007
Fragments arranged by size:
2319 2007 1328 1296 1053 184 51 36
32 14
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3243 10
Size: 54 1425 343 1411 5087
Fragments arranged by size:
5087 1425 1411 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4214 4515 6278 6637 6796 518
Size: 648 3048 301 1763 359 159 2042
Fragments arranged by size:
3048 2042 1763 648 359 301 159
MspA1I CmG'CkG
Cuts at: 1606 2507 3933 3983 4262 4372 4881 5566 5572
Size: 901 1426 50 279 110 509 685 6
Cuts at: 5572 5584 5593 5736 6413 7561 7806 1606
Size: 12 9 143 677 1148 245 2120
Fragments arranged by size:
2120 1426 1148 901 685 677 509 279
245 143 110 50 12 9 6
NarI GG'CG_CC
Cuts at: 5326 5326
Size: 8320
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6829
Fragments arranged by size:
6829 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8320
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8320
NotI GC'GGCC_GC
Cuts at: 5767 5767
Size: 8320
NsiI A_TGCA'T
Cuts at: 2217 2217
Size: 8320
NspI r_CATG'y
Cuts at: 1132 1229 2219 2903 2966 3724 5020 5678 7223
Size: 97 990 684 63 758 1296 658 1545
Cuts at: 7223 1132
Size: 2229
Fragments arranged by size:
2229 1545 1296 990 758 684 658 97
63
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8320
PciI A'CATG_T
Cuts at: 2899 7219 2899
Size: 4320 4000
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2231 1108
Size: 1123 7197
PfoI T'CCnGG_A
Cuts at: 4297 4297
Size: 8320
PmeI GTTT'AAAC
Cuts at: 5624 5624
Size: 8320
PmlI CAC'GTG
Cuts at: 4537 4537
Size: 8320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6389 6421 7902 7934 6389
Size: 32 1481 32 6775
Fragments arranged by size:
6775 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2166 2166
Size: 8320
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8320
PsiI TTA'TAA
Cuts at: 5890 5890
Size: 8320
PstI C_TGCA'G
Cuts at: 830 2603 3861 5183 5672 830
Size: 1773 1258 1322 489 3478
Fragments arranged by size:
3478 1773 1322 1258 489
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8320
PvuII CAG'CTG
Cuts at: 2507 3983 5566 5584 5593 2507
Size: 1476 1583 18 9 5234
Fragments arranged by size:
5234 1583 1476 18 9
SacI G_AGCT'C
Cuts at: 3399 4770 4911 3399
Size: 1371 141 6808
Fragments arranged by size:
6808 1371 141
SacII CC_GC'GG
Cuts at: 5573 5573
Size: 8320
SalI G'TCGA_C
Cuts at: 1936 5662 1936
Size: 3726 4594
SapI GCTCTTCn'nnn_
Cuts at: 4155 4404 4155
Size: 249 8071
SbfI CC_TGCA'GG
Cuts at: 5672 5672
Size: 8320
ScaI AGT'ACT
Cuts at: 6568 8280 6568
Size: 1712 6608
SfcI C'TryA_G
Cuts at: 826 1045 2440 2599 2867 2957 3857 3957 5179
Size: 219 1395 159 268 90 900 100 1222
Cuts at: 5179 5388 5668 6803 7484 7675 826
Size: 209 280 1135 681 191 1471
Fragments arranged by size:
1471 1395 1222 1135 900 681 280 268
219 209 191 159 100 90
SfoI GGC'GCC
Cuts at: 5327 5327
Size: 8320
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8320
SgrAI Cr'CCGG_yG
Cuts at: 1700 4775 1700
Size: 3075 5245
SmaI CCC'GGG
Cuts at: 1872 5647 1872
Size: 3775 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2341 3392 6424 7325 7587 820
Size: 197 926 398 1051 3032 901 262 1553
Fragments arranged by size:
3032 1553 1051 926 901 398 262 197
SnaBI TAC'GTA
Cuts at: 493 6111 493
Size: 5618 2702
SpeI A'CTAG_T
Cuts at: 152 5617 152
Size: 5465 2855
SphI G_CATG'C
Cuts at: 1132 3724 5020 5678 1132
Size: 2592 1296 658 3774
Fragments arranged by size:
3774 2592 1296 658
SspI AAT'ATT
Cuts at: 5 52 6244 5
Size: 47 6192 2081
Fragments arranged by size:
6192 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8320
StyI C'CwwG_G
Cuts at: 513 1065 2004 2364 4579 5190 5759 513
Size: 552 939 360 2215 611 569 3074
Fragments arranged by size:
3074 2215 939 611 569 552 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4810 6347 6364 6517 6702 7147 325
Size: 1255 3230 1537 17 153 185 445 1498
Fragments arranged by size:
3230 1537 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6566 8278 96
Size: 274 80 33 51 1443 4589 1712 138
Fragments arranged by size:
4589 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2387 2419 2835 2867 4526 4558 8280 8312 2387
Size: 32 416 32 1659 32 3722 32 2395
Fragments arranged by size:
3722 2395 1659 416 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4477 1316
Size: 3161 5159
XbaI T'CTAG_A
Cuts at: 3004 5656 3004
Size: 2652 5668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2133 4489 4882 2133
Size: 2356 393 5571
Fragments arranged by size:
5571 2356 393
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8320
XmaI C'CCGG_G
Cuts at: 1870 5645 1870
Size: 3775 4545
XmnI GAAnn'nnTTC
Cuts at: 2076 3120 3468 4058 6449 2076
Size: 1044 348 590 2391 3947
Fragments arranged by size:
3947 2391 1044 590 348
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6128 276
Size: 53 83 186 936 4594 2468
Fragments arranged by size:
4594 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BplI BsiWI BstZ17I EcoRV
FseI FspAI NaeI NgoMIV NruI PacI PspOMI PspXI
PsrI RsrII SanDI SexAI SfiI SrfI SwaI