Restriction map for
pFN21AA0605
(Circular) (Six-base) MAPSORT of: pFN21AA0605.seq Check: 9619 from: 1 to: 7558
pFN21AA0605 7558 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3749 3923 4439 3749
Size: 174 516 6868
Fragments arranged by size:
6868 516 174
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4638 5368 278
Size: 53 83 186 936 3102 730 2468
Fragments arranged by size:
3102 2468 936 730 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4901 1937
Size: 2964 4594
Acc65I G'GTAC_C
Cuts at: 2292 4879 2292
Size: 2587 4971
AclI AA'CG_TT
Cuts at: 1563 3603 5685 6058 1563
Size: 2040 2082 373 3063
Fragments arranged by size:
3063 2082 2040 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5621 7005 7273 1632
Size: 3989 1384 268 1917
Fragments arranged by size:
3989 1917 1384 268
AfeI AGC'GCT
Cuts at: 3052 3052
Size: 7558
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7361
AflIII A'CryG_T
Cuts at: 1203 2373 3696 4287 4547 6457 7434 1203
Size: 1170 1323 591 260 1910 977 1327
Fragments arranged by size:
1910 1327 1323 1170 977 591 260
AgeI A'CCGG_T
Cuts at: 3702 6389 3702
Size: 2687 4871
AhdI GACnn_n'nnGTC
Cuts at: 2077 6287 2077
Size: 4210 3348
AleI CACnn'nnGTG
Cuts at: 1166 2648 4368 1166
Size: 1482 1720 4356
Fragments arranged by size:
4356 1720 1482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3632 1184
Size: 2448 5110
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3889 3921 3889
Size: 32 7526
AlwNI CAG_nnn'CTG
Cuts at: 2271 2932 3067 3391 3404 3813 4587 5301 6873
Size: 661 135 324 13 409 774 714 1572
Cuts at: 6873 2271
Size: 2956
Fragments arranged by size:
2956 1572 774 714 661 409 324 135
13
ApaI G_GGCC'C
Cuts at: 2258 2919 3194 3258 4065 4208 2258
Size: 661 275 64 807 143 5608
Fragments arranged by size:
5608 807 661 275 143 64
ApaLI G'TGCA_C
Cuts at: 4093 4597 5615 6771 4093
Size: 504 1018 1156 4880
Fragments arranged by size:
4880 1156 1018 504
ApoI r'AATT_y
Cuts at: 1493 4867 5093 5245 7354 1493
Size: 3374 226 152 2109 1697
Fragments arranged by size:
3374 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6112 160
Size: 872 5080 1606
Fragments arranged by size:
5080 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7558
AvaI C'yCGr_G
Cuts at: 1870 1943 2251 2258 2721 3169 3833 4025 4508
Size: 73 308 7 463 448 664 192 483
Cuts at: 4508 4535 4883 1870
Size: 27 348 4545
Fragments arranged by size:
4545 664 483 463 448 348 308 192
73 27 7
BamHI G'GATC_C
Cuts at: 4888 5255 4888
Size: 367 7191
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2129 2292 2717 3458 4766
Size: 325 217 627 342 163 425 741 1308
Cuts at: 4766 4879 6334 618
Size: 113 1455 1842
Fragments arranged by size:
1842 1455 1308 741 627 425 342 325
217 163 113
BanII G_rGCy'C
Cuts at: 1391 2258 2565 2919 3194 3258 3473 3729 4065
Size: 867 307 354 275 64 215 256 336
Cuts at: 4065 4208 4521 4737 4877 1391
Size: 143 313 216 140 4072
Fragments arranged by size:
4072 867 354 336 313 307 275 256
216 215 143 140 64
BbeI G_GCGC'C
Cuts at: 3462 3462
Size: 7558
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6587
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2523 2557 3551 3585 4079 4113 4534
Size: 34 586 34 994 34 494 34 421
Cuts at: 4534 4568 5749 5783 1903
Size: 34 1181 34 3678
Fragments arranged by size:
3678 1181 994 586 494 421 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5450 6660 5450
Size: 1210 6348
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7558
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3171 6169 136
Size: 107 122 71 631 605 1499 2998 1525
Fragments arranged by size:
2998 1525 1499 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7553 7553
Size: 7558
BlpI GC'TnA_GC
Cuts at: 3554 4975 3554
Size: 1421 6137
Bme1580I G_kGCm'C
Cuts at: 1790 2132 2258 2282 2722 2919 3194 3258 4065
Size: 342 126 24 440 197 275 64 807
Cuts at: 4065 4097 4208 4601 4780 4809 5619 6775 1790
Size: 32 111 393 179 29 810 1156 2573
Fragments arranged by size:
2573 1156 810 807 440 393 342 275
197 179 126 111 64 32 29 24
BmgBI CAC'GTC
Cuts at: 1318 2283 2761 1318
Size: 965 478 6115
Fragments arranged by size:
6115 965 478
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4060 4249 4324
Size: 416 53 83 399 24 2643 189 75
Cuts at: 4324 4420 5289 6247 442
Size: 96 869 958 1753
Fragments arranged by size:
2643 1753 958 869 416 399 189 96
83 75 53 24
BmtI G_CTAG'C
Cuts at: 1056 2768 1056
Size: 1712 5846
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3379 3379
Size: 7558
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2419 3569 3916 6218 1239
Size: 902 278 1150 347 2302 2579
Fragments arranged by size:
2579 2302 1150 902 347 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5683 6548 6846 5683
Size: 865 298 6395
Fragments arranged by size:
6395 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2879 3203 3273 4337 6221 882
Size: 617 1380 324 70 1064 1884 2219
Fragments arranged by size:
2219 1884 1380 1064 617 324 70
BsaAI yAC'GTr
Cuts at: 493 1204 2374 2650 4288 4548 5349 493
Size: 711 1170 276 1638 260 801 2702
Fragments arranged by size:
2702 1638 1170 801 711 276 260
BsaBI GATnn'nnATC
Cuts at: 7552 7552
Size: 7558
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 3459 3792 3819
Size: 53 83 186 936 603 1323 333 27
Cuts at: 3819 4635 5365 5747 6382 275
Size: 816 730 382 635 1451
Fragments arranged by size:
1451 1323 936 816 730 635 603 382
333 186 83 53 27
BsaWI w'CCGG_w
Cuts at: 2888 3702 5991 6389 6663 6810 7221 2888
Size: 814 2289 398 274 147 411 3225
Fragments arranged by size:
3225 2289 814 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3092 3122 3283 3313 3332
Size: 30 165 30 1689 30 161 30 19
Cuts at: 3332 3362 3566 3596 3889 3919 4532 4562 1178
Size: 30 204 30 293 30 613 30 4174
Fragments arranged by size:
4174 1689 613 293 204 165 161 30
30 30 30 30 30 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2251 2347 2360 3118 3291 3518 3571 4553
Size: 1065 96 13 758 173 227 53 982
Cuts at: 4553 1186
Size: 4191
Fragments arranged by size:
4191 1065 982 758 227 173 96 53
13
BseYI C'CCAG_C
Cuts at: 2031 2066 3021 3139 3187 3194 3391 3857 6761
Size: 35 955 118 48 7 197 466 2904
Cuts at: 6761 7257 2031
Size: 496 2332
Fragments arranged by size:
2904 2332 955 496 466 197 118 48
35 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2257 2330 2438 2626 3727 4183 4270
Size: 573 521 73 108 188 1101 456 87
Cuts at: 4270 1163
Size: 4451
Fragments arranged by size:
4451 1101 573 521 456 188 108 87
73
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3630 5008 5769 6797 1540
Size: 275 186 1629 1378 761 1028 2301
Fragments arranged by size:
2301 1629 1378 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3473 4097 4464 4547 4601 5619 5704 6775 3473
Size: 624 367 83 54 1018 85 1071 4256
Fragments arranged by size:
4256 1071 1018 624 367 85 83 54
BsiWI C'GTAC_G
Cuts at: 3765 3765
Size: 7558
BsmI GAATG_Cn'
Cuts at: 2680 5069 5162 2680
Size: 2389 93 5076
Fragments arranged by size:
5076 2389 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2362 3878 4237 4626 2362
Size: 1516 359 389 5294
Fragments arranged by size:
5294 1516 389 359
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2132 2258 2282 2565 2722 2919 3194
Size: 399 342 126 24 283 157 197 275
Cuts at: 3194 3258 3473 3729 4065 4097 4208 4464 4521
Size: 64 215 256 336 32 111 256 57
Cuts at: 4521 4547 4601 4737 4780 4809 4877 5619 5704
Size: 26 54 136 43 29 68 742 85
Cuts at: 5704 6775 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 342 336 283 275
256 256 215 197 157 136 126 111
85 68 64 57 54 43 32 29
26 24
BspEI T'CCGG_A
Cuts at: 7221 7221
Size: 7558
BspHI T'CATG_A
Cuts at: 5447 7177 5447
Size: 1730 5828
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2279 2460 2538 3537 3749 3923 4049 4439
Size: 1435 181 78 999 212 174 126 390
Cuts at: 4439 4616 4913 844
Size: 177 297 3489
Fragments arranged by size:
3489 1435 999 390 297 212 181 177
174 126 78
BsrBI CCG'CTC
Cuts at: 1394 1823 2218 3136 4417 4836 5445 1394
Size: 429 395 918 1281 419 609 3507
Fragments arranged by size:
3507 1281 918 609 429 419 395
BsrDI GCAATG_nn'
Cuts at: 65 1248 2027 6053 6227 65
Size: 1183 779 4026 174 1396
Fragments arranged by size:
4026 1396 1183 779 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2862 3153 3233 3702 4218 6202 6389
Size: 110 1052 291 80 469 516 1984 187
Cuts at: 6389 1700
Size: 2869
Fragments arranged by size:
2869 1984 1052 516 469 291 187 110
80
BsrGI T'GTAC_A
Cuts at: 96 2376 3987 96
Size: 2280 1611 3667
Fragments arranged by size:
3667 2280 1611
BssHII G'CGCG_C
Cuts at: 1925 3668 1925
Size: 1743 5815
BssSI C'ACGA_G
Cuts at: 3008 3722 3845 5618 6630 3008
Size: 714 123 1773 1012 3936
Fragments arranged by size:
3936 1773 1012 714 123
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2227 2514 4041 4827 794
Size: 1433 287 1527 786 3525
Fragments arranged by size:
3525 1527 1433 786 287
BstBI TT'CG_AA
Cuts at: 6362 6362
Size: 7558
BstEII G'GTnAC_C
Cuts at: 1184 2698 4123 1184
Size: 1514 1425 4619
Fragments arranged by size:
4619 1514 1425
BstXI CCAn_nnnn'nTGG
Cuts at: 2119 4354 4370 2119
Size: 2235 16 5307
Fragments arranged by size:
5307 2235 16
BstYI r'GATC_y
Cuts at: 1972 4888 5255 5640 5657 7109 7195 7207 7553
Size: 2916 367 385 17 1452 86 12 346
Cuts at: 7553 1972
Size: 1977
Fragments arranged by size:
2916 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2091 2139 2466 2871 3690 4666
Size: 552 939 87 48 327 405 819 976
Cuts at: 4666 4728 513
Size: 62 3343
Fragments arranged by size:
3343 976 939 819 552 405 327 87
62 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2824 487
Size: 683 56 1598 5221
Fragments arranged by size:
5221 1598 683 56
BtsI GCAGTG_nn'
Cuts at: 2221 2357 3739 4095 4123 4229 4258 4507 5074
Size: 136 1382 356 28 106 29 249 567
Cuts at: 5074 5868 5888 2221
Size: 794 20 3891
Fragments arranged by size:
3891 1382 794 567 356 249 136 106
29 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7558
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7523
DraI TTT'AAA
Cuts at: 4862 4862
Size: 7558
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3287 6565 809
Size: 507 1971 3278 1802
Fragments arranged by size:
3278 1971 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2473 3118 3163
Size: 54 1425 50 275 18 643 645 45
Cuts at: 3163 3477 3627 4356 4476 4782 5005 5894 7523
Size: 314 150 729 120 306 223 889 1629
Cuts at: 7523 8
Size: 43
Fragments arranged by size:
1629 1425 889 729 645 643 314 306
275 223 150 120 54 50 45 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3627 5005 1537
Size: 275 1815 1378 4090
Fragments arranged by size:
4090 1815 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3321 5488 1353
Size: 1968 2167 3423
Fragments arranged by size:
3423 2167 1968
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2310 2401 6145 6519 6665 387
Size: 1064 138 721 91 3744 374 146 1280
Fragments arranged by size:
3744 1280 1064 721 374 146 138 91
EcoICRI GAG'CTC
Cuts at: 3471 3471
Size: 7558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2419 3569 3916 5621 6218 7005
Size: 393 509 278 1150 347 1705 597 787
Cuts at: 7005 7273 1239
Size: 268 1524
Fragments arranged by size:
1705 1524 1150 787 597 509 393 347
278 268
EcoNI CCTnn'n_nnAGG
Cuts at: 4001 4001
Size: 7558
EcoO109I rG'GnC_Cy
Cuts at: 2255 2268 2915 2929 3064 3191 3243 3254 3255
Size: 13 647 14 135 127 52 11 1
Cuts at: 3255 3540 3589 4062 4560 4845 2255
Size: 285 49 473 498 285 4968
Fragments arranged by size:
4968 647 498 473 285 285 135 127
52 49 14 13 11 1
EcoRI G'AATT_C
Cuts at: 4867 5245 4867
Size: 378 7180
FspI TGC'GCA
Cuts at: 6064 6064
Size: 7558
HaeII r_GCGC'y
Cuts at: 1678 3054 3462 6705 1678
Size: 1376 408 3243 2531
Fragments arranged by size:
3243 2531 1376 408
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2063 2095 2495 2527 4336
Size: 32 135 32 491 32 400 32 1809
Cuts at: 4336 4368 6199 6231 6273 6305 1373
Size: 32 1831 32 42 32 2626
Fragments arranged by size:
2626 1831 1809 491 400 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2082 3334 4902 5148 669
Size: 988 281 144 1252 1568 246 3079
Fragments arranged by size:
3079 1568 1252 988 281 246 144
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7558
HpaI GTT'AAC
Cuts at: 5148 5148
Size: 7558
KasI G'GCGC_C
Cuts at: 3458 3458
Size: 7558
KpnI G_GTAC'C
Cuts at: 2296 4883 2296
Size: 2587 4971
MfeI C'AATT_G
Cuts at: 5157 5157
Size: 7558
MluI A'CGCG_T
Cuts at: 7434 7434
Size: 7558
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4059 4260 6672 6856 1305
Size: 2754 201 2412 184 2007
Fragments arranged by size:
2754 2412 2007 201 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4358 4478 4784 10
Size: 54 1425 343 2526 120 306 2784
Fragments arranged by size:
2784 2526 1425 343 306 120 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2648 4368 4790 5516 5875 6034 518
Size: 648 1482 1720 422 726 359 159 2042
Fragments arranged by size:
2042 1720 1482 726 648 422 359 159
MspA1I CmG'CkG
Cuts at: 1606 2479 2640 2756 3388 3692 3894 4140 4749
Size: 873 161 116 632 304 202 246 609
Cuts at: 4749 4974 5651 6799 7044 1606
Size: 225 677 1148 245 2120
Fragments arranged by size:
2120 1148 873 677 632 609 304 246
245 225 202 161 116
NaeI GCC'GGC
Cuts at: 3235 3235
Size: 7558
NarI GG'CG_CC
Cuts at: 3459 3459
Size: 7558
NcoI C'CATG_G
Cuts at: 513 1065 2004 2871 513
Size: 552 939 867 5200
Fragments arranged by size:
5200 939 867 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7558
NgoMIV G'CCGG_C
Cuts at: 3233 3233
Size: 7558
NheI G'CTAG_C
Cuts at: 1052 2764 1052
Size: 1712 5846
NotI GC'GGCC_GC
Cuts at: 5005 5005
Size: 7558
NspI r_CATG'y
Cuts at: 1132 1229 3700 4916 6461 1132
Size: 97 2471 1216 1545 2229
Fragments arranged by size:
2471 2229 1545 1216 97
PasI CC'CwG_GG
Cuts at: 1348 3261 1348
Size: 1913 5645
PciI A'CATG_T
Cuts at: 3696 6457 3696
Size: 2761 4797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2602 3067 3391 4399 4587 1108
Size: 1494 465 324 1008 188 4079
Fragments arranged by size:
4079 1494 1008 465 324 188
PmeI GTTT'AAAC
Cuts at: 4862 4862
Size: 7558
PmlI CAC'GTG
Cuts at: 2374 2650 4288 4548 2374
Size: 276 1638 260 5384
Fragments arranged by size:
5384 1638 276 260
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3889 3921 5627 5659 7140 7172 3889
Size: 32 1706 32 1481 32 4275
Fragments arranged by size:
4275 1706 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2268 3540 2268
Size: 1272 6286
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7558
PsiI TTA'TAA
Cuts at: 5128 5128
Size: 7558
PspOMI G'GGCC_C
Cuts at: 2254 2915 3190 3254 4061 4204 2254
Size: 661 275 64 807 143 5608
Fragments arranged by size:
5608 807 661 275 143 64
PstI C_TGCA'G
Cuts at: 830 2232 2306 3000 3734 4719 4844 4910 830
Size: 1402 74 694 734 985 125 66 3478
Fragments arranged by size:
3478 1402 985 734 694 125 74 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7558
PvuII CAG'CTG
Cuts at: 2640 3388 3894 4749 2640
Size: 748 506 855 5449
Fragments arranged by size:
5449 855 748 506
SacI G_AGCT'C
Cuts at: 3473 3473
Size: 7558
SacII CC_GC'GG
Cuts at: 3693 3693
Size: 7558
SalI G'TCGA_C
Cuts at: 1936 4900 1936
Size: 2964 4594
SbfI CC_TGCA'GG
Cuts at: 4844 4910 4844
Size: 66 7492
ScaI AGT'ACT
Cuts at: 5806 7518 5806
Size: 1712 5846
SfcI C'TryA_G
Cuts at: 826 1045 2228 2302 2996 3730 4182 4715 4816
Size: 219 1183 74 694 734 452 533 101
Cuts at: 4816 4840 4906 6041 6722 6913 826
Size: 24 66 1135 681 191 1471
Fragments arranged by size:
1471 1183 1135 734 694 681 533 452
219 191 101 74 66 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 3171 3171
Size: 7558
SfoI GGC'GCC
Cuts at: 3460 3460
Size: 7558
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7558
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7558
SmaI CCC'GGG
Cuts at: 1872 2253 2260 3171 4885 1872
Size: 381 7 911 1714 4545
Fragments arranged by size:
4545 1714 911 381 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5662 6563 6825 820
Size: 197 926 3719 901 262 1553
Fragments arranged by size:
3719 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5349 493
Size: 4856 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7558
SphI G_CATG'C
Cuts at: 1132 4916 1132
Size: 3784 3774
SspI AAT'ATT
Cuts at: 5 52 5482 5
Size: 47 5430 2081
Fragments arranged by size:
5430 2081 47
StuI AGG'CCT
Cuts at: 1517 3125 4180 4706 1517
Size: 1608 1055 526 4369
Fragments arranged by size:
4369 1608 1055 526
StyI C'CwwG_G
Cuts at: 513 1065 2004 2683 2871 3464 3663 4997 513
Size: 552 939 679 188 593 199 1334 3074
Fragments arranged by size:
3074 1334 939 679 593 552 199 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2609 2660 3353 4670 5585 5602 5755
Size: 1255 1029 51 693 1317 915 17 153
Cuts at: 5755 5940 6385 325
Size: 185 445 1498
Fragments arranged by size:
1498 1317 1255 1029 915 693 445 185
153 51 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2376 2898 2985
Size: 274 80 33 51 1443 399 522 87
Cuts at: 2985 3987 4290 5804 7516 96
Size: 1002 303 1514 1712 138
Fragments arranged by size:
1712 1514 1443 1002 522 399 303 274
138 87 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3092 3124 3283 3315 3332 3364 4532 4564 7518
Size: 32 159 32 17 32 1168 32 2954
Cuts at: 7518 7550 3092
Size: 32 3100
Fragments arranged by size:
3100 2954 1168 159 32 32 32 32
32 17
Tth111I GACn'n_nGTC
Cuts at: 1316 2423 4661 1316
Size: 1107 2238 4213
Fragments arranged by size:
4213 2238 1107
XbaI T'CTAG_A
Cuts at: 4894 4894
Size: 7558
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2599 2711 4777 2599
Size: 112 2066 5380
Fragments arranged by size:
5380 2066 112
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7558
XmaI C'CCGG_G
Cuts at: 1870 2251 2258 3169 4883 1870
Size: 381 7 911 1714 4545
Fragments arranged by size:
4545 1714 911 381 7
XmnI GAAnn'nnTTC
Cuts at: 3416 5687 3416
Size: 2271 5287
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4636 5366 276
Size: 53 83 186 936 3102 730 2468
Fragments arranged by size:
3102 2468 936 730 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI Bpu10I BstZ17I Bsu36I
DraIII EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PspXI PsrI RsrII SanDI SapI SexAI SrfI
SwaI