Restriction map for
pFN21AA0599
(Circular) (Six-base) MAPSORT of: pFN21AA0599.seq Check: 990 from: 1 to: 8725
pFN21AA0599 8725 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2718 3372 2718
Size: 654 8071
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5517 6535 278
Size: 53 83 186 936 3981 1018 2468
Fragments arranged by size:
3981 2468 1018 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3018 5145 5269 6068 1937
Size: 1081 2127 124 799 4594
Fragments arranged by size:
4594 2127 1081 799 124
Acc65I G'GTAC_C
Cuts at: 3261 6046 3261
Size: 2785 5940
AclI AA'CG_TT
Cuts at: 1563 6852 7225 1563
Size: 5289 373 3063
Fragments arranged by size:
5289 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2414 2699 3305 3501 3948 4552 4785 5485
Size: 782 285 606 196 447 604 233 700
Cuts at: 5485 6788 8172 8440 1632
Size: 1303 1384 268 1917
Fragments arranged by size:
1917 1384 1303 782 700 606 604 447
285 268 233 196
AflII C'TTAA_G
Cuts at: 820 1017 4082 820
Size: 197 3065 5463
Fragments arranged by size:
5463 3065 197
AflIII A'CryG_T
Cuts at: 1203 7624 8601 1203
Size: 6421 977 1327
Fragments arranged by size:
6421 1327 977
AgeI A'CCGG_T
Cuts at: 4461 5875 7556 4461
Size: 1414 1681 5630
Fragments arranged by size:
5630 1681 1414
AhdI GACnn_n'nnGTC
Cuts at: 4240 7454 4240
Size: 3214 5511
AleI CACnn'nnGTG
Cuts at: 1166 3323 5215 1166
Size: 2157 1892 4676
Fragments arranged by size:
4676 2157 1892
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4304 4350 1184
Size: 3120 46 5559
Fragments arranged by size:
5559 3120 46
AlwNI CAG_nnn'CTG
Cuts at: 2205 2261 2338 2641 3479 4784 6468 8040 2205
Size: 56 77 303 838 1305 1684 1572 2890
Fragments arranged by size:
2890 1684 1572 1305 838 303 77 56
ApaI G_GGCC'C
Cuts at: 2226 2889 3945 4723 2226
Size: 663 1056 778 6228
Fragments arranged by size:
6228 1056 778 663
ApaLI G'TGCA_C
Cuts at: 6782 7938 6782
Size: 1156 7569
ApoI r'AATT_y
Cuts at: 1493 6034 6260 6412 8521 1493
Size: 4541 226 152 2109 1697
Fragments arranged by size:
4541 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7279 160
Size: 872 6247 1606
Fragments arranged by size:
6247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8725
AvaI C'yCGr_G
Cuts at: 1870 1943 2384 3390 3459 3759 4500 5010 5962
Size: 73 441 1006 69 300 741 510 952
Cuts at: 5962 6050 1870
Size: 88 4545
Fragments arranged by size:
4545 1006 952 741 510 441 300 88
73 69
BamHI G'GATC_C
Cuts at: 6055 6422 6055
Size: 367 8358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3261 3336 4121 5611 6046
Size: 325 217 627 1474 75 785 1490 435
Cuts at: 6046 7501 618
Size: 1455 1842
Fragments arranged by size:
1842 1490 1474 1455 785 627 435 325
217 75
BanII G_rGCy'C
Cuts at: 1391 2087 2121 2226 2669 2889 3627 3945 4262
Size: 696 34 105 443 220 738 318 317
Cuts at: 4262 4431 4703 4723 4734 4850 5255 5480 5526
Size: 169 272 20 11 116 405 225 46
Cuts at: 5526 6044 1391
Size: 518 4072
Fragments arranged by size:
4072 738 696 518 443 405 318 317
272 225 220 169 116 105 46 34
20 11
BbeI G_GCGC'C
Cuts at: 3340 3340
Size: 8725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4458 4827 928
Size: 971 2559 369 4826
Fragments arranged by size:
4826 2559 971 369
BbvCI CC'TCA_GC
Cuts at: 4349 5483 4349
Size: 1134 7591
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3841 3875 6916 6950 1903
Size: 34 50 34 1820 34 3041 34 3678
Fragments arranged by size:
3678 3041 1820 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6617 7827 6617
Size: 1210 7515
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8725
BfrBI ATG'CAT
Cuts at: 2595 2595
Size: 8725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4302 5006 5691
Size: 107 122 71 631 605 2630 704 685
Cuts at: 5691 5973 7336 136
Size: 282 1363 1525
Fragments arranged by size:
2630 1525 1363 704 685 631 605 282
122 107 71
BglII A'GATC_T
Cuts at: 4815 8720 4815
Size: 3905 4820
BlpI GC'TnA_GC
Cuts at: 2276 4292 5039 6142 2276
Size: 2016 747 1103 4859
Fragments arranged by size:
4859 2016 1103 747
Bme1580I G_kGCm'C
Cuts at: 1790 2226 2889 2927 3945 4723 5265 6786 7942
Size: 436 663 38 1018 778 542 1521 1156
Cuts at: 7942 1790
Size: 2573
Fragments arranged by size:
2573 1521 1156 1018 778 663 542 436
38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3813 3939 4442
Size: 416 53 83 399 24 2396 126 503
Cuts at: 4442 6456 7414 442
Size: 2014 958 1753
Fragments arranged by size:
2396 2014 1753 958 503 416 399 126
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8725
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4609 5545 4609
Size: 936 7789
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2182 2702 2834 2939 3596 3784 3963 4010
Size: 943 520 132 105 657 188 179 47
Cuts at: 4010 4319 4615 4659 4934 5304 5494 5801 5864
Size: 309 296 44 275 370 190 307 63
Cuts at: 5864 7385 1239
Size: 1521 2579
Fragments arranged by size:
2579 1521 943 657 520 370 309 307
296 275 190 188 179 132 105 63
47 44
Bpu10I CC'TnA_GC
Cuts at: 3368 4091 4349 5483 5669 3368
Size: 723 258 1134 186 6424
Fragments arranged by size:
6424 1134 723 258 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2684 6850 7715 8013 2684
Size: 4166 865 298 3396
Fragments arranged by size:
4166 3396 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2460 3574 3834 4497 5489 5744 7388
Size: 617 961 1114 260 663 992 255 1644
Cuts at: 7388 882
Size: 2219
Fragments arranged by size:
2219 1644 1114 992 961 663 617 260
255
BsaAI yAC'GTr
Cuts at: 493 1204 2329 5104 5119 6516 493
Size: 711 1125 2775 15 1397 2702
Fragments arranged by size:
2775 2702 1397 1125 711 15
BsaBI GATnn'nnATC
Cuts at: 3053 8719 3053
Size: 5666 3059
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3337 3959 5514 6532
Size: 53 83 186 936 1804 622 1555 1018
Cuts at: 6532 6914 7549 275
Size: 382 635 1451
Fragments arranged by size:
1804 1555 1451 1018 936 635 622 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4461 4838 5172 5875 7158 7556 7830 7977 8388
Size: 377 334 703 1283 398 274 147 411
Cuts at: 8388 4461
Size: 4798
Fragments arranged by size:
4798 1283 703 411 398 377 334 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 2555 2585 2846
Size: 30 165 30 777 30 345 30 261
Cuts at: 2846 2876 3961 3991 4613 4643 1178
Size: 30 1085 30 622 30 5260
Fragments arranged by size:
5260 1085 777 622 345 261 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2065 2077 2129 2450 2839 2858 3031 3158
Size: 879 12 52 321 389 19 173 127
Cuts at: 3158 3512 3527 3530 3542 3545 3548 3551 3554
Size: 354 15 3 12 3 3 3 3
Cuts at: 3554 3557 3560 3653 3656 3659 3662 3665 3674
Size: 3 3 93 3 3 3 3 9
Cuts at: 3674 3968 4115 4931 4958 4963 5371 5861 1186
Size: 294 147 816 27 5 408 490 4050
Fragments arranged by size:
4050 879 816 490 408 389 354 321
294 173 147 127 93 52 27 19
15 12 12 9 5 3 3 3
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2120 3614 3905 4927 5387 5825 5887 7928 8424
Size: 1494 291 1022 460 438 62 2041 496
Cuts at: 8424 2120
Size: 2421
Fragments arranged by size:
2421 2041 1494 1022 496 460 438 291
62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2247 5060 5111 1163
Size: 573 511 2813 51 4777
Fragments arranged by size:
4777 2813 573 511 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2051 4709 5575 6175 6936 7964
Size: 275 186 50 2658 866 600 761 1028
Cuts at: 7964 1540
Size: 2301
Fragments arranged by size:
2658 2301 1028 866 761 600 275 186
50
BsiHKAI G_wGCw'C
Cuts at: 2838 3041 4073 4293 4734 5364 5526 5675 6786
Size: 203 1032 220 441 630 162 149 1111
Cuts at: 6786 6871 7942 2838
Size: 85 1071 3621
Fragments arranged by size:
3621 1111 1071 1032 630 441 220 203
162 149 85
BsmI GAATG_Cn'
Cuts at: 2597 6236 6329 2597
Size: 3639 93 4993
Fragments arranged by size:
4993 3639 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3950 3950
Size: 8725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2121 2226 2669 2838 2889 2927
Size: 399 297 34 105 443 169 51 38
Cuts at: 2927 3041 3627 3945 4073 4262 4293 4431 4703
Size: 114 586 318 128 189 31 138 272
Cuts at: 4703 4723 4734 4850 5255 5265 5364 5480 5526
Size: 20 11 116 405 10 99 116 46
Cuts at: 5526 5675 6044 6786 6871 7942 1391
Size: 149 369 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 586 443 405 399 369
318 297 272 189 169 149 138 128
116 116 114 105 99 85 51 46
38 34 31 20 11 10
BspEI T'CCGG_A
Cuts at: 4838 5172 8388 4838
Size: 334 3216 5175
Fragments arranged by size:
5175 3216 334
BspHI T'CATG_A
Cuts at: 6614 8344 6614
Size: 1730 6995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2346 2682 2718 3372 3673 4899 5884 6080
Size: 1502 336 36 654 301 1226 985 196
Cuts at: 6080 844
Size: 3489
Fragments arranged by size:
3489 1502 1226 985 654 336 301 196
36
BsrBI CCG'CTC
Cuts at: 1394 1823 2047 3280 6612 1394
Size: 429 224 1233 3332 3507
Fragments arranged by size:
3507 3332 1233 429 224
BsrDI GCAATG_nn'
Cuts at: 65 1248 2956 4179 7220 7394 65
Size: 1183 1708 1223 3041 174 1396
Fragments arranged by size:
3041 1708 1396 1223 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2050 2169 2631 3339 3825 4461 4506
Size: 110 240 119 462 708 486 636 45
Cuts at: 4506 5317 5460 5574 5790 5875 7369 7556 1700
Size: 811 143 114 216 85 1494 187 2869
Fragments arranged by size:
2869 1494 811 708 636 486 462 240
216 187 143 119 114 110 85 45
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8725
BssSI C'ACGA_G
Cuts at: 6785 7797 6785
Size: 1012 7713
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2261 2641 2656 2944 3419 794
Size: 1467 380 15 288 475 6100
Fragments arranged by size:
6100 1467 475 380 288 15
BstBI TT'CG_AA
Cuts at: 7529 7529
Size: 8725
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8725
BstXI CCAn_nnnn'nTGG
Cuts at: 2033 2575 3139 4612 4880 5217 5714 2033
Size: 542 564 1473 268 337 497 5044
Fragments arranged by size:
5044 1473 564 542 497 337 268
BstYI r'GATC_y
Cuts at: 1972 4815 6055 6422 6807 6824 8276 8362 8374
Size: 2843 1240 367 385 17 1452 86 12
Cuts at: 8374 8720 1972
Size: 346 1977
Fragments arranged by size:
2843 1977 1452 1240 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3900 3900
Size: 8725
BtgI C'CryG_G
Cuts at: 513 1065 2109 2493 2574 4262 5581 513
Size: 552 1044 384 81 1688 1319 3657
Fragments arranged by size:
3657 1688 1319 1044 552 384 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2924 5562 487
Size: 683 56 1698 2638 3650
Fragments arranged by size:
3650 2638 1698 683 56
BtsI GCAGTG_nn'
Cuts at: 2110 2981 3424 3721 4339 4953 5072 5210 5222
Size: 871 443 297 618 614 119 138 12
Cuts at: 5222 5763 5832 5860 6241 7035 7055 2110
Size: 541 69 28 381 794 20 3780
Fragments arranged by size:
3780 871 794 618 614 541 443 381
297 138 119 69 28 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4228 4263 529
Size: 35 3664 35 4991
Fragments arranged by size:
4991 3664 35 35
DraI TTT'AAA
Cuts at: 6029 6029
Size: 8725
DraIII CAC_nnn'GTG
Cuts at: 5611 5611
Size: 8725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7732 809
Size: 507 6416 1802
Fragments arranged by size:
6416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2048 2502 2577
Size: 54 1425 50 275 18 218 454 75
Cuts at: 2577 3711 3823 4392 4652 4706 4950 5199 5572
Size: 1134 112 569 260 54 244 249 373
Cuts at: 5572 5857 6172 7061 8690 8
Size: 285 315 889 1629 43
Fragments arranged by size:
1629 1425 1134 889 569 454 373 315
285 275 260 249 244 218 112 75
54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2048 4706 5572 6172 1537
Size: 275 236 2658 866 600 4090
Fragments arranged by size:
4090 2658 866 600 275 236
EarI CTCTTCn'nnn_
Cuts at: 1353 2742 2816 3498 3504 3546 4910 5243 5271
Size: 1389 74 682 6 42 1364 333 28
Cuts at: 5271 5931 6655 1353
Size: 660 724 3423
Fragments arranged by size:
3423 1389 1364 724 682 660 333 74
42 28 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3692 4228 4781 5458 5709 7312
Size: 1064 138 2103 536 553 677 251 1603
Cuts at: 7312 7686 7832 387
Size: 374 146 1280
Fragments arranged by size:
2103 1603 1280 1064 677 553 536 374
251 146 138
EcoICRI GAG'CTC
Cuts at: 4732 5524 4732
Size: 792 7933
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2182 2414 2699 2702 2834 2939 3305
Size: 393 550 232 285 3 132 105 366
Cuts at: 3305 3501 3596 3784 3948 3963 4010 4319 4552
Size: 196 95 188 164 15 47 309 233
Cuts at: 4552 4615 4659 4785 4934 5304 5485 5494 5801
Size: 63 44 126 149 370 181 9 307
Cuts at: 5801 5864 6788 7385 8172 8440 1239
Size: 63 924 597 787 268 1524
Fragments arranged by size:
1524 924 787 597 550 393 370 366
309 307 285 268 233 232 196 188
181 164 149 132 126 105 95 63
63 47 44 15 9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2027 3881 2027
Size: 1854 6871
EcoO109I rG'GnC_Cy
Cuts at: 2222 2223 2258 2335 2885 2958 3463 3580 3941
Size: 1 35 77 550 73 505 117 361
Cuts at: 3941 4015 4078 4087 4315 4720 4762 5816 5950
Size: 74 63 9 228 405 42 1054 134
Cuts at: 5950 2222
Size: 4997
Fragments arranged by size:
4997 1054 550 505 405 361 228 134
117 77 74 73 63 42 35 9
1
EcoRI G'AATT_C
Cuts at: 6034 6412 6034
Size: 378 8347
EcoRV GAT'ATC
Cuts at: 2539 2539
Size: 8725
FseI GG_CCGG'CC
Cuts at: 5464 5578 5464
Size: 114 8611
FspI TGC'GCA
Cuts at: 2342 2948 7231 2342
Size: 606 4283 3836
Fragments arranged by size:
4283 3836 606
HaeII r_GCGC'y
Cuts at: 1678 2415 3340 3768 7872 1678
Size: 737 925 428 4104 2531
Fragments arranged by size:
4104 2531 925 737 428
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2846 2878 3299 3331 3959
Size: 32 135 32 1274 32 421 32 628
Cuts at: 3959 3991 4226 4258 7366 7398 7440 7472 1373
Size: 32 235 32 3108 32 42 32 2626
Fragments arranged by size:
3108 2626 1274 628 421 235 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5596 6069 6315 669
Size: 988 281 3658 473 246 3079
Fragments arranged by size:
3658 3079 988 473 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8725
HpaI GTT'AAC
Cuts at: 6315 6315
Size: 8725
KasI G'GCGC_C
Cuts at: 3336 3336
Size: 8725
KpnI G_GTAC'C
Cuts at: 3265 6050 3265
Size: 2785 5940
MfeI C'AATT_G
Cuts at: 6324 6324
Size: 8725
MluI A'CGCG_T
Cuts at: 8601 8601
Size: 8725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3857 7839 8023 1305
Size: 2552 3982 184 2007
Fragments arranged by size:
3982 2552 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2504 4394 5201 5859 10
Size: 54 1425 343 672 1890 807 658 2876
Fragments arranged by size:
2876 1890 1425 807 672 658 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2031 2739 3323 4332 4382 4557
Size: 648 843 22 708 584 1009 50 175
Cuts at: 4557 5215 6683 7042 7201 518
Size: 658 1468 359 159 2042
Fragments arranged by size:
2042 1468 1009 843 708 658 648 584
359 175 159 50 22
MspA1I CmG'CkG
Cuts at: 1606 2205 2252 2478 2508 3267 4064 4264 4353
Size: 599 47 226 30 759 797 200 89
Cuts at: 4353 5152 5347 5505 5583 5658 6141 6818 7966
Size: 799 195 158 78 75 483 677 1148
Cuts at: 7966 8211 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 799 797 759 677 599 483
245 226 200 195 158 89 78 75
47 30
NaeI GCC'GGC
Cuts at: 2171 3341 5462 5576 2171
Size: 1170 2121 114 5320
Fragments arranged by size:
5320 2121 1170 114
NarI GG'CG_CC
Cuts at: 3337 3337
Size: 8725
NcoI C'CATG_G
Cuts at: 513 1065 2109 513
Size: 552 1044 7129
Fragments arranged by size:
7129 1044 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8725
NgoMIV G'CCGG_C
Cuts at: 2169 3339 5460 5574 2169
Size: 1170 2121 114 5320
Fragments arranged by size:
5320 2121 1170 114
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8725
NotI GC'GGCC_GC
Cuts at: 6172 6172
Size: 8725
NsiI A_TGCA'T
Cuts at: 2597 2597
Size: 8725
NspI r_CATG'y
Cuts at: 1132 1229 2326 2599 3413 6083 7628 1132
Size: 97 1097 273 814 2670 1545 2229
Fragments arranged by size:
2670 2229 1545 1097 814 273 97
PasI CC'CwG_GG
Cuts at: 1348 4850 5053 5714 1348
Size: 3502 203 661 4359
Fragments arranged by size:
4359 3502 661 203
PciI A'CATG_T
Cuts at: 7624 7624
Size: 8725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2205 5107 5297 1108
Size: 1097 2902 190 4536
Fragments arranged by size:
4536 2902 1097 190
PfoI T'CCnGG_A
Cuts at: 2718 2850 2916 3432 3800 3987 4911 2718
Size: 132 66 516 368 187 924 6532
Fragments arranged by size:
6532 924 516 368 187 132 66
PmeI GTTT'AAAC
Cuts at: 6029 6029
Size: 8725
PmlI CAC'GTG
Cuts at: 5119 5119
Size: 8725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6794 6826 8307 8339 6794
Size: 32 1481 32 7180
Fragments arranged by size:
7180 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2335 2958 3580 4078 4762 5816 5950 2335
Size: 623 622 498 684 1054 134 5110
Fragments arranged by size:
5110 1054 684 623 622 498 134
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8725
PsiI TTA'TAA
Cuts at: 6295 6295
Size: 8725
PspOMI G'GGCC_C
Cuts at: 2222 2885 3941 4719 2222
Size: 663 1056 778 6228
Fragments arranged by size:
6228 1056 778 663
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5129 5161 5129
Size: 32 8693
PstI C_TGCA'G
Cuts at: 830 3272 3471 5217 5770 5839 5920 6077 830
Size: 2442 199 1746 553 69 81 157 3478
Fragments arranged by size:
3478 2442 1746 553 199 157 81 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8725
PvuII CAG'CTG
Cuts at: 2205 2478 2508 4064 4353 5152 5347 5505 2205
Size: 273 30 1556 289 799 195 158 5425
Fragments arranged by size:
5425 1556 799 289 273 195 158 30
RsrII CG'GwC_CG
Cuts at: 2842 2842
Size: 8725
SacI G_AGCT'C
Cuts at: 4734 5526 4734
Size: 792 7933
SacII CC_GC'GG
Cuts at: 4265 5584 4265
Size: 1319 7406
SalI G'TCGA_C
Cuts at: 1936 6067 1936
Size: 4131 4594
SanDI GG'GwC_CC
Cuts at: 4762 5816 5950 4762
Size: 1054 134 7537
Fragments arranged by size:
7537 1054 134
SapI GCTCTTCn'nnn_
Cuts at: 3546 5243 3546
Size: 1697 7028
SbfI CC_TGCA'GG
Cuts at: 6077 6077
Size: 8725
ScaI AGT'ACT
Cuts at: 3841 6973 8685 3841
Size: 3132 1712 3881
Fragments arranged by size:
3881 3132 1712
SfcI C'TryA_G
Cuts at: 826 1045 2645 3268 3467 5213 5270 5766 5835
Size: 219 1600 623 199 1746 57 496 69
Cuts at: 5835 5916 6073 7208 7889 8080 826
Size: 81 157 1135 681 191 1471
Fragments arranged by size:
1746 1600 1471 1135 681 623 496 219
199 191 157 81 69 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 5006 5006
Size: 8725
SfoI GGC'GCC
Cuts at: 3338 3338
Size: 8725
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8725
SgrAI Cr'CCGG_yG
Cuts at: 1700 5875 1700
Size: 4175 4550
SmaI CCC'GGG
Cuts at: 1872 2386 3761 5012 6052 1872
Size: 514 1375 1251 1040 4545
Fragments arranged by size:
4545 1375 1251 1040 514
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2699 4082 6829 7730 7992 820
Size: 197 926 756 1383 2747 901 262 1553
Fragments arranged by size:
2747 1553 1383 926 901 756 262 197
SnaBI TAC'GTA
Cuts at: 493 2329 6516 493
Size: 1836 4187 2702
Fragments arranged by size:
4187 2702 1836
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8725
SphI G_CATG'C
Cuts at: 1132 2599 3413 6083 1132
Size: 1467 814 2670 3774
Fragments arranged by size:
3774 2670 1467 814
SspI AAT'ATT
Cuts at: 5 52 6649 5
Size: 47 6597 2081
Fragments arranged by size:
6597 2081 47
StuI AGG'CCT
Cuts at: 1517 3560 6008 1517
Size: 2043 2448 4234
Fragments arranged by size:
4234 2448 2043
StyI C'CwwG_G
Cuts at: 513 1065 2109 2661 3219 6164 513
Size: 552 1044 552 558 2945 3074
Fragments arranged by size:
3074 2945 1044 558 552 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3107 6752 6769 6922 7107 7552 325
Size: 1255 1527 3645 17 153 185 445 1498
Fragments arranged by size:
3645 1527 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3117 3839 5163
Size: 274 80 33 51 1443 1140 722 1324
Cuts at: 5163 6971 8683 96
Size: 1808 1712 138
Fragments arranged by size:
1808 1712 1443 1324 1140 722 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3961 3993 4893 4925 8685 8717 3961
Size: 32 900 32 3760 32 3969
Fragments arranged by size:
3969 3760 900 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8725
XbaI T'CTAG_A
Cuts at: 6061 6061
Size: 8725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2512 2794 3235 3706 5214 5839 2512
Size: 282 441 471 1508 625 5398
Fragments arranged by size:
5398 1508 625 471 441 282
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8725
XmaI C'CCGG_G
Cuts at: 1870 2384 3759 5010 6050 1870
Size: 514 1375 1251 1040 4545
Fragments arranged by size:
4545 1375 1251 1040 514
XmnI GAAnn'nnTTC
Cuts at: 6854 6854
Size: 8725
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5515 6533 276
Size: 53 83 186 936 3981 1018 2468
Fragments arranged by size:
3981 2468 1018 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BstZ17I FalI
FspAI NruI PacI PspXI SexAI SrfI SwaI