(Circular) (Six-base) MAPSORT of: pFN21AA0599.seq Check: 990 from: 1 to: 8725 pFN21AA0599 8725 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2718 3372 2718 Size: 654 8071 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5517 6535 278 Size: 53 83 186 936 3981 1018 2468 Fragments arranged by size: 3981 2468 1018 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3018 5145 5269 6068 1937 Size: 1081 2127 124 799 4594 Fragments arranged by size: 4594 2127 1081 799 124 Acc65I G'GTAC_C Cuts at: 3261 6046 3261 Size: 2785 5940 AclI AA'CG_TT Cuts at: 1563 6852 7225 1563 Size: 5289 373 3063 Fragments arranged by size: 5289 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2414 2699 3305 3501 3948 4552 4785 5485 Size: 782 285 606 196 447 604 233 700 Cuts at: 5485 6788 8172 8440 1632 Size: 1303 1384 268 1917 Fragments arranged by size: 1917 1384 1303 782 700 606 604 447 285 268 233 196 AflII C'TTAA_G Cuts at: 820 1017 4082 820 Size: 197 3065 5463 Fragments arranged by size: 5463 3065 197 AflIII A'CryG_T Cuts at: 1203 7624 8601 1203 Size: 6421 977 1327 Fragments arranged by size: 6421 1327 977 AgeI A'CCGG_T Cuts at: 4461 5875 7556 4461 Size: 1414 1681 5630 Fragments arranged by size: 5630 1681 1414 AhdI GACnn_n'nnGTC Cuts at: 4240 7454 4240 Size: 3214 5511 AleI CACnn'nnGTG Cuts at: 1166 3323 5215 1166 Size: 2157 1892 4676 Fragments arranged by size: 4676 2157 1892 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4304 4350 1184 Size: 3120 46 5559 Fragments arranged by size: 5559 3120 46 AlwNI CAG_nnn'CTG Cuts at: 2205 2261 2338 2641 3479 4784 6468 8040 2205 Size: 56 77 303 838 1305 1684 1572 2890 Fragments arranged by size: 2890 1684 1572 1305 838 303 77 56 ApaI G_GGCC'C Cuts at: 2226 2889 3945 4723 2226 Size: 663 1056 778 6228 Fragments arranged by size: 6228 1056 778 663 ApaLI G'TGCA_C Cuts at: 6782 7938 6782 Size: 1156 7569 ApoI r'AATT_y Cuts at: 1493 6034 6260 6412 8521 1493 Size: 4541 226 152 2109 1697 Fragments arranged by size: 4541 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7279 160 Size: 872 6247 1606 Fragments arranged by size: 6247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8725 AvaI C'yCGr_G Cuts at: 1870 1943 2384 3390 3459 3759 4500 5010 5962 Size: 73 441 1006 69 300 741 510 952 Cuts at: 5962 6050 1870 Size: 88 4545 Fragments arranged by size: 4545 1006 952 741 510 441 300 88 73 69 BamHI G'GATC_C Cuts at: 6055 6422 6055 Size: 367 8358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3261 3336 4121 5611 6046 Size: 325 217 627 1474 75 785 1490 435 Cuts at: 6046 7501 618 Size: 1455 1842 Fragments arranged by size: 1842 1490 1474 1455 785 627 435 325 217 75 BanII G_rGCy'C Cuts at: 1391 2087 2121 2226 2669 2889 3627 3945 4262 Size: 696 34 105 443 220 738 318 317 Cuts at: 4262 4431 4703 4723 4734 4850 5255 5480 5526 Size: 169 272 20 11 116 405 225 46 Cuts at: 5526 6044 1391 Size: 518 4072 Fragments arranged by size: 4072 738 696 518 443 405 318 317 272 225 220 169 116 105 46 34 20 11 BbeI G_GCGC'C Cuts at: 3340 3340 Size: 8725 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4458 4827 928 Size: 971 2559 369 4826 Fragments arranged by size: 4826 2559 971 369 BbvCI CC'TCA_GC Cuts at: 4349 5483 4349 Size: 1134 7591 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3841 3875 6916 6950 1903 Size: 34 50 34 1820 34 3041 34 3678 Fragments arranged by size: 3678 3041 1820 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6617 7827 6617 Size: 1210 7515 BclI T'GATC_A Cuts at: 1548 1548 Size: 8725 BfrBI ATG'CAT Cuts at: 2595 2595 Size: 8725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4302 5006 5691 Size: 107 122 71 631 605 2630 704 685 Cuts at: 5691 5973 7336 136 Size: 282 1363 1525 Fragments arranged by size: 2630 1525 1363 704 685 631 605 282 122 107 71 BglII A'GATC_T Cuts at: 4815 8720 4815 Size: 3905 4820 BlpI GC'TnA_GC Cuts at: 2276 4292 5039 6142 2276 Size: 2016 747 1103 4859 Fragments arranged by size: 4859 2016 1103 747 Bme1580I G_kGCm'C Cuts at: 1790 2226 2889 2927 3945 4723 5265 6786 7942 Size: 436 663 38 1018 778 542 1521 1156 Cuts at: 7942 1790 Size: 2573 Fragments arranged by size: 2573 1521 1156 1018 778 663 542 436 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8725 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3813 3939 4442 Size: 416 53 83 399 24 2396 126 503 Cuts at: 4442 6456 7414 442 Size: 2014 958 1753 Fragments arranged by size: 2396 2014 1753 958 503 416 399 126 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8725 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4609 5545 4609 Size: 936 7789 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2182 2702 2834 2939 3596 3784 3963 4010 Size: 943 520 132 105 657 188 179 47 Cuts at: 4010 4319 4615 4659 4934 5304 5494 5801 5864 Size: 309 296 44 275 370 190 307 63 Cuts at: 5864 7385 1239 Size: 1521 2579 Fragments arranged by size: 2579 1521 943 657 520 370 309 307 296 275 190 188 179 132 105 63 47 44 Bpu10I CC'TnA_GC Cuts at: 3368 4091 4349 5483 5669 3368 Size: 723 258 1134 186 6424 Fragments arranged by size: 6424 1134 723 258 186 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2684 6850 7715 8013 2684 Size: 4166 865 298 3396 Fragments arranged by size: 4166 3396 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2460 3574 3834 4497 5489 5744 7388 Size: 617 961 1114 260 663 992 255 1644 Cuts at: 7388 882 Size: 2219 Fragments arranged by size: 2219 1644 1114 992 961 663 617 260 255 BsaAI yAC'GTr Cuts at: 493 1204 2329 5104 5119 6516 493 Size: 711 1125 2775 15 1397 2702 Fragments arranged by size: 2775 2702 1397 1125 711 15 BsaBI GATnn'nnATC Cuts at: 3053 8719 3053 Size: 5666 3059 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3337 3959 5514 6532 Size: 53 83 186 936 1804 622 1555 1018 Cuts at: 6532 6914 7549 275 Size: 382 635 1451 Fragments arranged by size: 1804 1555 1451 1018 936 635 622 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4461 4838 5172 5875 7158 7556 7830 7977 8388 Size: 377 334 703 1283 398 274 147 411 Cuts at: 8388 4461 Size: 4798 Fragments arranged by size: 4798 1283 703 411 398 377 334 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 2555 2585 2846 Size: 30 165 30 777 30 345 30 261 Cuts at: 2846 2876 3961 3991 4613 4643 1178 Size: 30 1085 30 622 30 5260 Fragments arranged by size: 5260 1085 777 622 345 261 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2065 2077 2129 2450 2839 2858 3031 3158 Size: 879 12 52 321 389 19 173 127 Cuts at: 3158 3512 3527 3530 3542 3545 3548 3551 3554 Size: 354 15 3 12 3 3 3 3 Cuts at: 3554 3557 3560 3653 3656 3659 3662 3665 3674 Size: 3 3 93 3 3 3 3 9 Cuts at: 3674 3968 4115 4931 4958 4963 5371 5861 1186 Size: 294 147 816 27 5 408 490 4050 Fragments arranged by size: 4050 879 816 490 408 389 354 321 294 173 147 127 93 52 27 19 15 12 12 9 5 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2120 3614 3905 4927 5387 5825 5887 7928 8424 Size: 1494 291 1022 460 438 62 2041 496 Cuts at: 8424 2120 Size: 2421 Fragments arranged by size: 2421 2041 1494 1022 496 460 438 291 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2247 5060 5111 1163 Size: 573 511 2813 51 4777 Fragments arranged by size: 4777 2813 573 511 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2051 4709 5575 6175 6936 7964 Size: 275 186 50 2658 866 600 761 1028 Cuts at: 7964 1540 Size: 2301 Fragments arranged by size: 2658 2301 1028 866 761 600 275 186 50 BsiHKAI G_wGCw'C Cuts at: 2838 3041 4073 4293 4734 5364 5526 5675 6786 Size: 203 1032 220 441 630 162 149 1111 Cuts at: 6786 6871 7942 2838 Size: 85 1071 3621 Fragments arranged by size: 3621 1111 1071 1032 630 441 220 203 162 149 85 BsmI GAATG_Cn' Cuts at: 2597 6236 6329 2597 Size: 3639 93 4993 Fragments arranged by size: 4993 3639 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3950 3950 Size: 8725 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2121 2226 2669 2838 2889 2927 Size: 399 297 34 105 443 169 51 38 Cuts at: 2927 3041 3627 3945 4073 4262 4293 4431 4703 Size: 114 586 318 128 189 31 138 272 Cuts at: 4703 4723 4734 4850 5255 5265 5364 5480 5526 Size: 20 11 116 405 10 99 116 46 Cuts at: 5526 5675 6044 6786 6871 7942 1391 Size: 149 369 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 586 443 405 399 369 318 297 272 189 169 149 138 128 116 116 114 105 99 85 51 46 38 34 31 20 11 10 BspEI T'CCGG_A Cuts at: 4838 5172 8388 4838 Size: 334 3216 5175 Fragments arranged by size: 5175 3216 334 BspHI T'CATG_A Cuts at: 6614 8344 6614 Size: 1730 6995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2346 2682 2718 3372 3673 4899 5884 6080 Size: 1502 336 36 654 301 1226 985 196 Cuts at: 6080 844 Size: 3489 Fragments arranged by size: 3489 1502 1226 985 654 336 301 196 36 BsrBI CCG'CTC Cuts at: 1394 1823 2047 3280 6612 1394 Size: 429 224 1233 3332 3507 Fragments arranged by size: 3507 3332 1233 429 224 BsrDI GCAATG_nn' Cuts at: 65 1248 2956 4179 7220 7394 65 Size: 1183 1708 1223 3041 174 1396 Fragments arranged by size: 3041 1708 1396 1223 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2050 2169 2631 3339 3825 4461 4506 Size: 110 240 119 462 708 486 636 45 Cuts at: 4506 5317 5460 5574 5790 5875 7369 7556 1700 Size: 811 143 114 216 85 1494 187 2869 Fragments arranged by size: 2869 1494 811 708 636 486 462 240 216 187 143 119 114 110 85 45 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8725 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8725 BssSI C'ACGA_G Cuts at: 6785 7797 6785 Size: 1012 7713 BstAPI GCAn_nnn'nTGC Cuts at: 794 2261 2641 2656 2944 3419 794 Size: 1467 380 15 288 475 6100 Fragments arranged by size: 6100 1467 475 380 288 15 BstBI TT'CG_AA Cuts at: 7529 7529 Size: 8725 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8725 BstXI CCAn_nnnn'nTGG Cuts at: 2033 2575 3139 4612 4880 5217 5714 2033 Size: 542 564 1473 268 337 497 5044 Fragments arranged by size: 5044 1473 564 542 497 337 268 BstYI r'GATC_y Cuts at: 1972 4815 6055 6422 6807 6824 8276 8362 8374 Size: 2843 1240 367 385 17 1452 86 12 Cuts at: 8374 8720 1972 Size: 346 1977 Fragments arranged by size: 2843 1977 1452 1240 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3900 3900 Size: 8725 BtgI C'CryG_G Cuts at: 513 1065 2109 2493 2574 4262 5581 513 Size: 552 1044 384 81 1688 1319 3657 Fragments arranged by size: 3657 1688 1319 1044 552 384 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2924 5562 487 Size: 683 56 1698 2638 3650 Fragments arranged by size: 3650 2638 1698 683 56 BtsI GCAGTG_nn' Cuts at: 2110 2981 3424 3721 4339 4953 5072 5210 5222 Size: 871 443 297 618 614 119 138 12 Cuts at: 5222 5763 5832 5860 6241 7035 7055 2110 Size: 541 69 28 381 794 20 3780 Fragments arranged by size: 3780 871 794 618 614 541 443 381 297 138 119 69 28 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4228 4263 529 Size: 35 3664 35 4991 Fragments arranged by size: 4991 3664 35 35 DraI TTT'AAA Cuts at: 6029 6029 Size: 8725 DraIII CAC_nnn'GTG Cuts at: 5611 5611 Size: 8725 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7732 809 Size: 507 6416 1802 Fragments arranged by size: 6416 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2048 2502 2577 Size: 54 1425 50 275 18 218 454 75 Cuts at: 2577 3711 3823 4392 4652 4706 4950 5199 5572 Size: 1134 112 569 260 54 244 249 373 Cuts at: 5572 5857 6172 7061 8690 8 Size: 285 315 889 1629 43 Fragments arranged by size: 1629 1425 1134 889 569 454 373 315 285 275 260 249 244 218 112 75 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2048 4706 5572 6172 1537 Size: 275 236 2658 866 600 4090 Fragments arranged by size: 4090 2658 866 600 275 236 EarI CTCTTCn'nnn_ Cuts at: 1353 2742 2816 3498 3504 3546 4910 5243 5271 Size: 1389 74 682 6 42 1364 333 28 Cuts at: 5271 5931 6655 1353 Size: 660 724 3423 Fragments arranged by size: 3423 1389 1364 724 682 660 333 74 42 28 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3692 4228 4781 5458 5709 7312 Size: 1064 138 2103 536 553 677 251 1603 Cuts at: 7312 7686 7832 387 Size: 374 146 1280 Fragments arranged by size: 2103 1603 1280 1064 677 553 536 374 251 146 138 EcoICRI GAG'CTC Cuts at: 4732 5524 4732 Size: 792 7933 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2182 2414 2699 2702 2834 2939 3305 Size: 393 550 232 285 3 132 105 366 Cuts at: 3305 3501 3596 3784 3948 3963 4010 4319 4552 Size: 196 95 188 164 15 47 309 233 Cuts at: 4552 4615 4659 4785 4934 5304 5485 5494 5801 Size: 63 44 126 149 370 181 9 307 Cuts at: 5801 5864 6788 7385 8172 8440 1239 Size: 63 924 597 787 268 1524 Fragments arranged by size: 1524 924 787 597 550 393 370 366 309 307 285 268 233 232 196 188 181 164 149 132 126 105 95 63 63 47 44 15 9 3 EcoNI CCTnn'n_nnAGG Cuts at: 2027 3881 2027 Size: 1854 6871 EcoO109I rG'GnC_Cy Cuts at: 2222 2223 2258 2335 2885 2958 3463 3580 3941 Size: 1 35 77 550 73 505 117 361 Cuts at: 3941 4015 4078 4087 4315 4720 4762 5816 5950 Size: 74 63 9 228 405 42 1054 134 Cuts at: 5950 2222 Size: 4997 Fragments arranged by size: 4997 1054 550 505 405 361 228 134 117 77 74 73 63 42 35 9 1 EcoRI G'AATT_C Cuts at: 6034 6412 6034 Size: 378 8347 EcoRV GAT'ATC Cuts at: 2539 2539 Size: 8725 FseI GG_CCGG'CC Cuts at: 5464 5578 5464 Size: 114 8611 FspI TGC'GCA Cuts at: 2342 2948 7231 2342 Size: 606 4283 3836 Fragments arranged by size: 4283 3836 606 HaeII r_GCGC'y Cuts at: 1678 2415 3340 3768 7872 1678 Size: 737 925 428 4104 2531 Fragments arranged by size: 4104 2531 925 737 428 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2846 2878 3299 3331 3959 Size: 32 135 32 1274 32 421 32 628 Cuts at: 3959 3991 4226 4258 7366 7398 7440 7472 1373 Size: 32 235 32 3108 32 42 32 2626 Fragments arranged by size: 3108 2626 1274 628 421 235 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5596 6069 6315 669 Size: 988 281 3658 473 246 3079 Fragments arranged by size: 3658 3079 988 473 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8725 HpaI GTT'AAC Cuts at: 6315 6315 Size: 8725 KasI G'GCGC_C Cuts at: 3336 3336 Size: 8725 KpnI G_GTAC'C Cuts at: 3265 6050 3265 Size: 2785 5940 MfeI C'AATT_G Cuts at: 6324 6324 Size: 8725 MluI A'CGCG_T Cuts at: 8601 8601 Size: 8725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3857 7839 8023 1305 Size: 2552 3982 184 2007 Fragments arranged by size: 3982 2552 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2504 4394 5201 5859 10 Size: 54 1425 343 672 1890 807 658 2876 Fragments arranged by size: 2876 1890 1425 807 672 658 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2031 2739 3323 4332 4382 4557 Size: 648 843 22 708 584 1009 50 175 Cuts at: 4557 5215 6683 7042 7201 518 Size: 658 1468 359 159 2042 Fragments arranged by size: 2042 1468 1009 843 708 658 648 584 359 175 159 50 22 MspA1I CmG'CkG Cuts at: 1606 2205 2252 2478 2508 3267 4064 4264 4353 Size: 599 47 226 30 759 797 200 89 Cuts at: 4353 5152 5347 5505 5583 5658 6141 6818 7966 Size: 799 195 158 78 75 483 677 1148 Cuts at: 7966 8211 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 799 797 759 677 599 483 245 226 200 195 158 89 78 75 47 30 NaeI GCC'GGC Cuts at: 2171 3341 5462 5576 2171 Size: 1170 2121 114 5320 Fragments arranged by size: 5320 2121 1170 114 NarI GG'CG_CC Cuts at: 3337 3337 Size: 8725 NcoI C'CATG_G Cuts at: 513 1065 2109 513 Size: 552 1044 7129 Fragments arranged by size: 7129 1044 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8725 NgoMIV G'CCGG_C Cuts at: 2169 3339 5460 5574 2169 Size: 1170 2121 114 5320 Fragments arranged by size: 5320 2121 1170 114 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8725 NotI GC'GGCC_GC Cuts at: 6172 6172 Size: 8725 NsiI A_TGCA'T Cuts at: 2597 2597 Size: 8725 NspI r_CATG'y Cuts at: 1132 1229 2326 2599 3413 6083 7628 1132 Size: 97 1097 273 814 2670 1545 2229 Fragments arranged by size: 2670 2229 1545 1097 814 273 97 PasI CC'CwG_GG Cuts at: 1348 4850 5053 5714 1348 Size: 3502 203 661 4359 Fragments arranged by size: 4359 3502 661 203 PciI A'CATG_T Cuts at: 7624 7624 Size: 8725 PflMI CCAn_nnn'nTGG Cuts at: 1108 2205 5107 5297 1108 Size: 1097 2902 190 4536 Fragments arranged by size: 4536 2902 1097 190 PfoI T'CCnGG_A Cuts at: 2718 2850 2916 3432 3800 3987 4911 2718 Size: 132 66 516 368 187 924 6532 Fragments arranged by size: 6532 924 516 368 187 132 66 PmeI GTTT'AAAC Cuts at: 6029 6029 Size: 8725 PmlI CAC'GTG Cuts at: 5119 5119 Size: 8725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6794 6826 8307 8339 6794 Size: 32 1481 32 7180 Fragments arranged by size: 7180 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2335 2958 3580 4078 4762 5816 5950 2335 Size: 623 622 498 684 1054 134 5110 Fragments arranged by size: 5110 1054 684 623 622 498 134 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8725 PsiI TTA'TAA Cuts at: 6295 6295 Size: 8725 PspOMI G'GGCC_C Cuts at: 2222 2885 3941 4719 2222 Size: 663 1056 778 6228 Fragments arranged by size: 6228 1056 778 663 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5129 5161 5129 Size: 32 8693 PstI C_TGCA'G Cuts at: 830 3272 3471 5217 5770 5839 5920 6077 830 Size: 2442 199 1746 553 69 81 157 3478 Fragments arranged by size: 3478 2442 1746 553 199 157 81 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8725 PvuII CAG'CTG Cuts at: 2205 2478 2508 4064 4353 5152 5347 5505 2205 Size: 273 30 1556 289 799 195 158 5425 Fragments arranged by size: 5425 1556 799 289 273 195 158 30 RsrII CG'GwC_CG Cuts at: 2842 2842 Size: 8725 SacI G_AGCT'C Cuts at: 4734 5526 4734 Size: 792 7933 SacII CC_GC'GG Cuts at: 4265 5584 4265 Size: 1319 7406 SalI G'TCGA_C Cuts at: 1936 6067 1936 Size: 4131 4594 SanDI GG'GwC_CC Cuts at: 4762 5816 5950 4762 Size: 1054 134 7537 Fragments arranged by size: 7537 1054 134 SapI GCTCTTCn'nnn_ Cuts at: 3546 5243 3546 Size: 1697 7028 SbfI CC_TGCA'GG Cuts at: 6077 6077 Size: 8725 ScaI AGT'ACT Cuts at: 3841 6973 8685 3841 Size: 3132 1712 3881 Fragments arranged by size: 3881 3132 1712 SfcI C'TryA_G Cuts at: 826 1045 2645 3268 3467 5213 5270 5766 5835 Size: 219 1600 623 199 1746 57 496 69 Cuts at: 5835 5916 6073 7208 7889 8080 826 Size: 81 157 1135 681 191 1471 Fragments arranged by size: 1746 1600 1471 1135 681 623 496 219 199 191 157 81 69 57 SfiI GGCCn_nnn'nGGCC Cuts at: 5006 5006 Size: 8725 SfoI GGC'GCC Cuts at: 3338 3338 Size: 8725 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8725 SgrAI Cr'CCGG_yG Cuts at: 1700 5875 1700 Size: 4175 4550 SmaI CCC'GGG Cuts at: 1872 2386 3761 5012 6052 1872 Size: 514 1375 1251 1040 4545 Fragments arranged by size: 4545 1375 1251 1040 514 SmlI C'TyrA_G Cuts at: 820 1017 1943 2699 4082 6829 7730 7992 820 Size: 197 926 756 1383 2747 901 262 1553 Fragments arranged by size: 2747 1553 1383 926 901 756 262 197 SnaBI TAC'GTA Cuts at: 493 2329 6516 493 Size: 1836 4187 2702 Fragments arranged by size: 4187 2702 1836 SpeI A'CTAG_T Cuts at: 152 152 Size: 8725 SphI G_CATG'C Cuts at: 1132 2599 3413 6083 1132 Size: 1467 814 2670 3774 Fragments arranged by size: 3774 2670 1467 814 SspI AAT'ATT Cuts at: 5 52 6649 5 Size: 47 6597 2081 Fragments arranged by size: 6597 2081 47 StuI AGG'CCT Cuts at: 1517 3560 6008 1517 Size: 2043 2448 4234 Fragments arranged by size: 4234 2448 2043 StyI C'CwwG_G Cuts at: 513 1065 2109 2661 3219 6164 513 Size: 552 1044 552 558 2945 3074 Fragments arranged by size: 3074 2945 1044 558 552 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3107 6752 6769 6922 7107 7552 325 Size: 1255 1527 3645 17 153 185 445 1498 Fragments arranged by size: 3645 1527 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3117 3839 5163 Size: 274 80 33 51 1443 1140 722 1324 Cuts at: 5163 6971 8683 96 Size: 1808 1712 138 Fragments arranged by size: 1808 1712 1443 1324 1140 722 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3961 3993 4893 4925 8685 8717 3961 Size: 32 900 32 3760 32 3969 Fragments arranged by size: 3969 3760 900 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8725 XbaI T'CTAG_A Cuts at: 6061 6061 Size: 8725 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2512 2794 3235 3706 5214 5839 2512 Size: 282 441 471 1508 625 5398 Fragments arranged by size: 5398 1508 625 471 441 282 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8725 XmaI C'CCGG_G Cuts at: 1870 2384 3759 5010 6050 1870 Size: 514 1375 1251 1040 4545 Fragments arranged by size: 4545 1375 1251 1040 514 XmnI GAAnn'nnTTC Cuts at: 6854 6854 Size: 8725 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5515 6533 276 Size: 53 83 186 936 3981 1018 2468 Fragments arranged by size: 3981 2468 1018 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsiWI BstZ17I FalI FspAI NruI PacI PspXI SexAI SrfI SwaI