Restriction map for
pFN21AA0596
(Circular) (Six-base) MAPSORT of: pFN21AA0596.seq Check: 1262 from: 1 to: 9247
pFN21AA0596 9247 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7057 278
Size: 53 83 186 936 5521 2468
Fragments arranged by size:
5521 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3195 4869 6590 1937
Size: 1258 1674 1721 4594
Fragments arranged by size:
4594 1721 1674 1258
Acc65I G'GTAC_C
Cuts at: 6568 6568
Size: 9247
AclI AA'CG_TT
Cuts at: 1563 7374 7747 1563
Size: 5811 373 3063
Fragments arranged by size:
5811 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3512 3569 4413 5070 6375 7310 8694 8962
Size: 1880 57 844 657 1305 935 1384 268
Cuts at: 8962 1632
Size: 1917
Fragments arranged by size:
1917 1880 1384 1305 935 844 657 268
57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9050
AflIII A'CryG_T
Cuts at: 1203 2487 8146 9123 1203
Size: 1284 5659 977 1327
Fragments arranged by size:
5659 1327 1284 977
AgeI A'CCGG_T
Cuts at: 8078 8078
Size: 9247
AhdI GACnn_n'nnGTC
Cuts at: 7976 7976
Size: 9247
AleI CACnn'nnGTG
Cuts at: 1166 2711 1166
Size: 1545 7702
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2361 1184
Size: 1177 8070
AlwNI CAG_nnn'CTG
Cuts at: 2153 3079 3382 3535 3691 4234 4538 4998 5022
Size: 926 303 153 156 543 304 460 24
Cuts at: 5022 5498 5538 6108 6121 6406 6484 6990 8562
Size: 476 40 570 13 285 78 506 1572
Cuts at: 8562 2153
Size: 2838
Fragments arranged by size:
2838 1572 926 570 543 506 476 460
304 303 285 156 153 78 40 24
13
ApaI G_GGCC'C
Cuts at: 5159 5257 5968 6099 5159
Size: 98 711 131 8307
Fragments arranged by size:
8307 711 131 98
ApaLI G'TGCA_C
Cuts at: 3518 5600 7304 8460 3518
Size: 2082 1704 1156 4305
Fragments arranged by size:
4305 2082 1704 1156
ApoI r'AATT_y
Cuts at: 1493 2606 4133 6556 6782 6934 9043 1493
Size: 1113 1527 2423 226 152 2109 1697
Fragments arranged by size:
2423 2109 1697 1527 1113 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7801 160
Size: 872 6769 1606
Fragments arranged by size:
6769 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9247
AvaI C'yCGr_G
Cuts at: 1870 1943 3200 4932 5670 6572 1870
Size: 73 1257 1732 738 902 4545
Fragments arranged by size:
4545 1732 1257 902 738 73
AvrII C'CTAG_G
Cuts at: 6004 6068 6004
Size: 64 9183
BamHI G'GATC_C
Cuts at: 3433 6577 6944 3433
Size: 3144 367 5736
Fragments arranged by size:
5736 3144 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2903 4194 4314 5389 5426
Size: 325 217 627 1116 1291 120 1075 37
Cuts at: 5426 5611 6053 6568 8023 618
Size: 185 442 515 1455 1842
Fragments arranged by size:
1842 1455 1291 1116 1075 627 515 442
325 217 185 120 37
BanII G_rGCy'C
Cuts at: 1391 3296 3882 4205 4973 5073 5159 5257 5545
Size: 1905 586 323 768 100 86 98 288
Cuts at: 5545 5702 5887 5968 6099 6282 6566 1391
Size: 157 185 81 131 183 284 4072
Fragments arranged by size:
4072 1905 768 586 323 288 284 185
183 157 131 100 98 86 81
BbeI G_GCGC'C
Cuts at: 4198 4198
Size: 9247
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3857 5058 6185 928
Size: 971 1958 1201 1127 3990
Fragments arranged by size:
3990 1958 1201 1127 971
BbvCI CC'TCA_GC
Cuts at: 2108 2819 3332 5115 6343 2108
Size: 711 513 1783 1228 5012
Fragments arranged by size:
5012 1783 1228 711 513
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4431 4465 5355 5389 7438 7472 1903
Size: 34 2494 34 890 34 2049 34 3678
Fragments arranged by size:
3678 2494 2049 890 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3045 4894 7139 8349 3045
Size: 1849 2245 1210 3943
Fragments arranged by size:
3943 2245 1849 1210
BclI T'GATC_A
Cuts at: 1548 3756 1548
Size: 2208 7039
BfrBI ATG'CAT
Cuts at: 3422 3422
Size: 9247
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4756 7858 136
Size: 107 122 71 631 605 3084 3102 1525
Fragments arranged by size:
3102 3084 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2054 4834 9242 2054
Size: 2780 4408 2059
Fragments arranged by size:
4408 2780 2059
BlpI GC'TnA_GC
Cuts at: 6664 6664
Size: 9247
Bme1580I G_kGCm'C
Cuts at: 1790 2340 2658 3509 3522 4507 5159 5257 5604
Size: 550 318 851 13 985 652 98 347
Cuts at: 5604 5968 6056 6099 6115 7308 8464 1790
Size: 364 88 43 16 1193 1156 2573
Fragments arranged by size:
2573 1193 1156 985 851 652 550 364
347 318 98 88 43 16 13
BmgBI CAC'GTC
Cuts at: 1318 2365 2760 1318
Size: 1047 395 7805
Fragments arranged by size:
7805 1047 395
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3231 4498 5252
Size: 416 53 83 399 24 1814 1267 754
Cuts at: 5252 5587 6978 7936 442
Size: 335 1391 958 1753
Fragments arranged by size:
1814 1753 1391 1267 958 754 416 399
335 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2988 3623 1056
Size: 1932 635 6680
Fragments arranged by size:
6680 1932 635
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2123 6514 2123
Size: 4391 4856
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2175 2346 3036 3202 3423 3605 3828 4911
Size: 936 171 690 166 221 182 223 1083
Cuts at: 4911 5175 5232 5460 5567 6272 6509 7907 1239
Size: 264 57 228 107 705 237 1398 2579
Fragments arranged by size:
2579 1398 1083 936 705 690 264 237
228 223 221 182 171 166 107 57
Bpu10I CC'TnA_GC
Cuts at: 2108 2671 2819 2942 3332 4199 5115 5685 6221
Size: 563 148 123 390 867 916 570 536
Cuts at: 6221 6343 2108
Size: 122 5012
Fragments arranged by size:
5012 916 867 570 563 536 390 148
123 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4739 5341 5811 7372 8237 8535 4739
Size: 602 470 1561 865 298 5451
Fragments arranged by size:
5451 1561 865 602 470 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5371 7910 882
Size: 617 3872 2539 2219
Fragments arranged by size:
3872 2539 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3841 7038 493
Size: 711 2637 3197 2702
Fragments arranged by size:
3197 2702 2637 711
BsaBI GATnn'nnATC
Cuts at: 2059 4214 9241 2059
Size: 2155 5027 2065
Fragments arranged by size:
5027 2155 2065
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4195 7054 7436 8071
Size: 53 83 186 936 2662 2859 382 635
Cuts at: 8071 275
Size: 1451
Fragments arranged by size:
2859 2662 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7680 8078 8352 8499 8910 7680
Size: 398 274 147 411 8017
Fragments arranged by size:
8017 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 2390 2420 3692
Size: 30 165 30 693 30 264 30 1272
Cuts at: 3692 3722 3801 3831 1178
Size: 30 79 30 6594
Fragments arranged by size:
6594 1272 693 264 165 79 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3247 3420 4066 5083 5156 5344 6362 1186
Size: 2061 173 646 1017 73 188 1018 4071
Fragments arranged by size:
4071 2061 1018 1017 646 188 173 73
BseYI C'CCAG_C
Cuts at: 2137 2203 2662 2680 3881 4457 4462 4531 4572
Size: 66 459 18 1201 576 5 69 41
Cuts at: 4572 5477 5484 5538 5710 5876 6098 6114 6366
Size: 905 7 54 172 166 222 16 252
Cuts at: 6366 6419 8450 8946 2137
Size: 53 2031 496 2438
Fragments arranged by size:
2438 2031 1201 905 576 496 459 252
222 172 166 69 66 54 53 41
18 16 7 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2918 3839 4025 4830 4940 5282 1163
Size: 573 1182 921 186 805 110 342 5128
Fragments arranged by size:
5128 1182 921 805 573 342 186 110
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6697 7458 8486 1540
Size: 275 186 4696 761 1028 2301
Fragments arranged by size:
4696 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2376 2403 3296 3522 4205 4973 5043 5066 5446
Size: 27 893 226 683 768 70 23 380
Cuts at: 5446 5604 6282 7308 7393 8464 2376
Size: 158 678 1026 85 1071 3159
Fragments arranged by size:
3159 1071 1026 893 768 683 678 380
226 158 85 70 27 23
BsmI GAATG_Cn'
Cuts at: 2650 3031 5939 6203 6758 6851 2650
Size: 381 2908 264 555 93 5046
Fragments arranged by size:
5046 2908 555 381 264 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5525 5733 5525
Size: 208 9039
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2340 2376 2403 2658 3296 3509 3522
Size: 399 550 36 27 255 638 213 13
Cuts at: 3522 3882 4205 4507 4973 5043 5066 5073 5159
Size: 360 323 302 466 70 23 7 86
Cuts at: 5159 5257 5446 5545 5604 5702 5887 5968 6056
Size: 98 189 99 59 98 185 81 88
Cuts at: 6056 6099 6115 6282 6566 7308 7393 8464 1391
Size: 43 16 167 284 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 638 550 466 399 360
323 302 284 255 213 189 185 167
99 98 98 88 86 85 81 70
59 43 36 27 23 16 13 7
BspEI T'CCGG_A
Cuts at: 8910 8910
Size: 9247
BspHI T'CATG_A
Cuts at: 7136 8866 7136
Size: 1730 7517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2940 3260 4852 5254 6602 844
Size: 2096 320 1592 402 1348 3489
Fragments arranged by size:
3489 2096 1592 1348 402 320
BsrBI CCG'CTC
Cuts at: 1394 1823 2670 3453 7134 1394
Size: 429 847 783 3681 3507
Fragments arranged by size:
3681 3507 847 783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2979 3045 4029 5428 7742 7916 65
Size: 1183 1731 66 984 1399 2314 174 1396
Fragments arranged by size:
2314 1731 1399 1396 1183 984 174 66
BsrFI r'CCGG_y
Cuts at: 1700 1810 3639 4311 4345 7891 8078 1700
Size: 110 1829 672 34 3546 187 2869
Fragments arranged by size:
3546 2869 1829 672 187 110 34
BsrGI T'GTAC_A
Cuts at: 96 3090 96
Size: 2994 6253
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9247
BssSI C'ACGA_G
Cuts at: 7307 8319 7307
Size: 1012 8235
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5022 794
Size: 4228 5019
BstBI TT'CG_AA
Cuts at: 8051 8051
Size: 9247
BstEII G'GTnAC_C
Cuts at: 1184 2119 1184
Size: 935 8312
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3139 5554 5767 6193 2011
Size: 1128 2415 213 426 5065
Fragments arranged by size:
5065 2415 1128 426 213
BstYI r'GATC_y
Cuts at: 1972 2054 3433 4023 4604 4615 4834 6577 6944
Size: 82 1379 590 581 11 219 1743 367
Cuts at: 6944 7329 7346 8798 8884 8896 9242 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1743 1452 1379 590 581 385 367
346 219 86 82 17 12 11
Bsu36I CC'TnA_GG
Cuts at: 2764 4018 4078 4761 2764
Size: 1254 60 683 7250
Fragments arranged by size:
7250 1254 683 60
BtgI C'CryG_G
Cuts at: 513 1065 2004 4515 4896 5766 6192 6283 6519
Size: 552 939 2511 381 870 426 91 236
Cuts at: 6519 513
Size: 3241
Fragments arranged by size:
3241 2511 939 870 552 426 381 236
91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8508
Fragments arranged by size:
8508 683 56
BtsI GCAGTG_nn'
Cuts at: 2283 2556 2583 2854 3343 3714 3792 3949 4409
Size: 273 27 271 489 371 78 157 460
Cuts at: 4409 5660 5912 6263 6309 6432 6763 7557 7577
Size: 1251 252 351 46 123 331 794 20
Cuts at: 7577 2283
Size: 3953
Fragments arranged by size:
3953 1251 794 489 460 371 351 331
273 271 252 157 123 78 46 27
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2829 2864 4084 4119 529
Size: 35 2265 35 1220 35 5657
Fragments arranged by size:
5657 2265 1220 35 35 35
DraI TTT'AAA
Cuts at: 3967 6551 3967
Size: 2584 6663
DraIII CAC_nnn'GTG
Cuts at: 3841 3844 3980 6322 3841
Size: 3 136 2342 6766
Fragments arranged by size:
6766 2342 136 3
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2480 2551 8254 809
Size: 507 1164 71 5703 1802
Fragments arranged by size:
5703 1802 1164 507 71
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2385 4092 4105
Size: 54 1425 50 275 18 555 1707 13
Cuts at: 4105 4236 4674 5232 5556 5769 6694 7583 9212
Size: 131 438 558 324 213 925 889 1629
Cuts at: 9212 8
Size: 43
Fragments arranged by size:
1707 1629 1425 925 889 558 555 438
324 275 213 131 54 50 43 18
13
EagI C'GGCC_G
Cuts at: 1537 1812 6694 1537
Size: 275 4882 4090
Fragments arranged by size:
4882 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3014 4558 4942 5100 5124 5133 5302 6143
Size: 1661 1544 384 158 24 9 169 841
Cuts at: 6143 6394 7177 1353
Size: 251 783 3423
Fragments arranged by size:
3423 1661 1544 841 783 384 251 169
158 24 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2753 3263 7834 8208 8354 387
Size: 1064 138 1164 510 4571 374 146 1280
Fragments arranged by size:
4571 1280 1164 1064 510 374 146 138
EcoICRI GAG'CTC
Cuts at: 3294 4203 4971 6280 3294
Size: 909 768 1309 6261
Fragments arranged by size:
6261 1309 909 768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2175 2346 3036 3202 3423 3512 3569
Size: 393 543 171 690 166 221 89 57
Cuts at: 3569 3605 3828 4413 4911 5070 5175 5232 5460
Size: 36 223 585 498 159 105 57 228
Cuts at: 5460 5567 6272 6375 6509 7310 7907 8694 8962
Size: 107 705 103 134 801 597 787 268
Cuts at: 8962 1239
Size: 1524
Fragments arranged by size:
1524 801 787 705 690 597 585 543
498 393 268 228 223 221 171 166
159 134 107 105 103 89 57 57
36
EcoNI CCTnn'n_nnAGG
Cuts at: 5652 6224 5652
Size: 572 8675
EcoO109I rG'GnC_Cy
Cuts at: 2104 3127 3379 4321 4553 4654 4714 5156 5253
Size: 1023 252 942 232 101 60 442 97
Cuts at: 5253 5254 5266 5644 5965 6146 6481 2104
Size: 1 12 378 321 181 335 4870
Fragments arranged by size:
4870 1023 942 442 378 335 321 252
232 181 101 97 60 12 1
EcoRI G'AATT_C
Cuts at: 6556 6934 6556
Size: 378 8869
EcoRV GAT'ATC
Cuts at: 5778 5778
Size: 9247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2140 4418 6007 2140
Size: 2278 1589 5380
Fragments arranged by size:
5380 2278 1589
FspI TGC'GCA
Cuts at: 2075 3798 7753 2075
Size: 1723 3955 3569
Fragments arranged by size:
3955 3569 1723
HaeII r_GCGC'y
Cuts at: 1678 2442 4198 4573 8394 1678
Size: 764 1756 375 3821 2531
Fragments arranged by size:
3821 2531 1756 764 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2029 2061 2096 2128 2168
Size: 32 135 32 457 32 35 32 40
Cuts at: 2168 2200 3046 3078 3686 3718 4061 4093 4216
Size: 32 846 32 608 32 343 32 123
Cuts at: 4216 4248 4335 4367 5075 5107 5505 5537 5668
Size: 32 87 32 708 32 398 32 131
Cuts at: 5668 5700 6371 6403 7888 7920 7962 7994 1373
Size: 32 671 32 1485 32 42 32 2626
Fragments arranged by size:
2626 1485 846 708 671 608 457 398
343 135 131 123 87 42 40 35
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2022 2485 6591 6837 669
Size: 988 281 84 463 4106 246 3079
Fragments arranged by size:
4106 3079 988 463 281 246 84
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9247
HpaI GTT'AAC
Cuts at: 6837 6837
Size: 9247
KasI G'GCGC_C
Cuts at: 4194 4194
Size: 9247
KpnI G_GTAC'C
Cuts at: 6572 6572
Size: 9247
MfeI C'AATT_G
Cuts at: 6846 6846
Size: 9247
MluI A'CGCG_T
Cuts at: 9123 9123
Size: 9247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2547 3411 6338 8361 8545 1305
Size: 1242 864 2927 2023 184 2007
Fragments arranged by size:
2927 2023 2007 1242 864 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4094 4107 4676 5234 5558
Size: 54 1425 343 2262 13 569 558 324
Cuts at: 5558 5771 10
Size: 213 3486
Fragments arranged by size:
3486 2262 1425 569 558 343 324 213
54 13
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2406 2513 2711 2864 3359 3872
Size: 648 843 397 107 198 153 495 513
Cuts at: 3872 4001 4127 4163 4187 4220 7205 7564 7723
Size: 129 126 36 24 33 2985 359 159
Cuts at: 7723 518
Size: 2042
Fragments arranged by size:
2985 2042 843 648 513 495 397 359
198 159 153 129 126 107 36 33
24
MspA1I CmG'CkG
Cuts at: 1606 3762 3885 4041 4179 4258 4627 5022 5114
Size: 2156 123 156 138 79 369 395 92
Cuts at: 5114 5538 5714 6242 6268 6285 6663 7340 8488
Size: 424 176 528 26 17 378 677 1148
Cuts at: 8488 8733 1606
Size: 245 2120
Fragments arranged by size:
2156 2120 1148 677 528 424 395 378
369 245 176 156 138 123 92 79
26 17
NarI GG'CG_CC
Cuts at: 4195 4195
Size: 9247
NcoI C'CATG_G
Cuts at: 513 1065 2004 4896 5766 6192 513
Size: 552 939 2892 870 426 3568
Fragments arranged by size:
3568 2892 939 870 552 426
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9247
NheI G'CTAG_C
Cuts at: 1052 2984 3619 1052
Size: 1932 635 6680
Fragments arranged by size:
6680 1932 635
NotI GC'GGCC_GC
Cuts at: 6694 6694
Size: 9247
NsiI A_TGCA'T
Cuts at: 3424 3424
Size: 9247
NspI r_CATG'y
Cuts at: 1132 1229 2344 6605 8150 1132
Size: 97 1115 4261 1545 2229
Fragments arranged by size:
4261 2229 1545 1115 97
PasI CC'CwG_GG
Cuts at: 1348 6452 1348
Size: 5104 4143
PciI A'CATG_T
Cuts at: 8146 8146
Size: 9247
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2968 3655 4837 5717 6322 1108
Size: 1860 687 1182 880 605 4033
Fragments arranged by size:
4033 1860 1182 880 687 605
PfoI T'CCnGG_A
Cuts at: 2270 4339 4524 5895 2270
Size: 2069 185 1371 5622
Fragments arranged by size:
5622 2069 1371 185
PmeI GTTT'AAAC
Cuts at: 6551 6551
Size: 9247
PmlI CAC'GTG
Cuts at: 3841 3841
Size: 9247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6528 6560 7316 7348 8829 8861 6528
Size: 32 756 32 1481 32 6914
Fragments arranged by size:
6914 1481 756 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 3127 4553 4654 5266 2104
Size: 1023 1426 101 612 6085
Fragments arranged by size:
6085 1426 1023 612 101
PshAI GACnn'nnGTC
Cuts at: 1613 3606 1613
Size: 1993 7254
PsiI TTA'TAA
Cuts at: 6817 6817
Size: 9247
PspOMI G'GGCC_C
Cuts at: 5155 5253 5964 6095 5155
Size: 98 711 131 8307
Fragments arranged by size:
8307 711 131 98
PstI C_TGCA'G
Cuts at: 830 2397 2590 4543 4653 5643 6599 830
Size: 1567 193 1953 110 990 956 3478
Fragments arranged by size:
3478 1953 1567 990 956 193 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9247
PvuII CAG'CTG
Cuts at: 3762 3885 4041 4179 4627 5022 5114 5538 5714
Size: 123 156 138 448 395 92 424 176
Cuts at: 5714 6268 3762
Size: 554 6741
Fragments arranged by size:
6741 554 448 424 395 176 156 138
123 92
SacI G_AGCT'C
Cuts at: 3296 4205 4973 6282 3296
Size: 909 768 1309 6261
Fragments arranged by size:
6261 1309 909 768
SacII CC_GC'GG
Cuts at: 6286 6286
Size: 9247
SalI G'TCGA_C
Cuts at: 1936 6589 1936
Size: 4653 4594
SanDI GG'GwC_CC
Cuts at: 3127 3127
Size: 9247
SapI GCTCTTCn'nnn_
Cuts at: 5100 5100
Size: 9247
SbfI CC_TGCA'GG
Cuts at: 4653 6599 4653
Size: 1946 7301
ScaI AGT'ACT
Cuts at: 2315 7495 9207 2315
Size: 5180 1712 2355
Fragments arranged by size:
5180 2355 1712
SexAI A'CCwGG_T
Cuts at: 5889 5889
Size: 9247
SfcI C'TryA_G
Cuts at: 826 1045 2393 2586 3680 4539 4649 5168 5639
Size: 219 1348 193 1094 859 110 519 471
Cuts at: 5639 6595 7730 8411 8602 826
Size: 956 1135 681 191 1471
Fragments arranged by size:
1471 1348 1135 1094 956 859 681 519
471 219 193 191 110
SfoI GGC'GCC
Cuts at: 4196 4196
Size: 9247
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9247
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9247
SmaI CCC'GGG
Cuts at: 1872 4934 6574 1872
Size: 3062 1640 4545
Fragments arranged by size:
4545 3062 1640
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4718 5356 5826 7351 8252 8514
Size: 197 926 2775 638 470 1525 901 262
Cuts at: 8514 820
Size: 1553
Fragments arranged by size:
2775 1553 1525 926 901 638 470 262
197
SnaBI TAC'GTA
Cuts at: 493 7038 493
Size: 6545 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9247
SphI G_CATG'C
Cuts at: 1132 6605 1132
Size: 5473 3774
SspI AAT'ATT
Cuts at: 5 52 3922 7171 5
Size: 47 3870 3249 2081
Fragments arranged by size:
3870 3249 2081 47
StuI AGG'CCT
Cuts at: 1517 3224 1517
Size: 1707 7540
StyI C'CwwG_G
Cuts at: 513 1065 2004 2115 2124 4896 5766 6004 6068
Size: 552 939 111 9 2772 870 238 64
Cuts at: 6068 6142 6192 6686 513
Size: 74 50 494 3074
Fragments arranged by size:
3074 2772 939 870 552 494 238 111
74 64 50 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2800 3389 3930 4441 4864 7274 7291
Size: 1255 1220 589 541 511 423 2410 17
Cuts at: 7291 7444 7629 8074 325
Size: 153 185 445 1498
Fragments arranged by size:
2410 1498 1255 1220 589 541 511 445
423 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2313 2445 3090
Size: 274 80 33 51 1443 336 132 645
Cuts at: 3090 3150 3672 3891 5317 6020 7493 9205 96
Size: 60 522 219 1426 703 1473 1712 138
Fragments arranged by size:
1712 1473 1443 1426 703 645 522 336
274 219 138 132 80 60 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2133 2165 3692 3724 3801 3833 4401 4433 9207
Size: 32 1527 32 77 32 568 32 4774
Cuts at: 9207 9239 2133
Size: 32 2141
Fragments arranged by size:
4774 2141 1527 568 77 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9247
XbaI T'CTAG_A
Cuts at: 6583 6583
Size: 9247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2122 4298 4441 5764 6089 6323 2122
Size: 2176 143 1323 325 234 5046
Fragments arranged by size:
5046 2176 1323 325 234 143
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9247
XmaI C'CCGG_G
Cuts at: 1870 4932 6572 1870
Size: 3062 1640 4545
Fragments arranged by size:
4545 3062 1640
XmnI GAAnn'nnTTC
Cuts at: 4401 7376 4401
Size: 2975 6272
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7055 276
Size: 53 83 186 936 5521 2468
Fragments arranged by size:
5521 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BsiWI BstZ17I FseI
FspAI NaeI NgoMIV NruI PacI PspXI PsrI RsrII
SfiI SrfI SwaI