Restriction map for
pFN21AA0584
(Circular) (Six-base) MAPSORT of: pFN21AA0584.seq Check: 4523 from: 1 to: 6583
pFN21AA0584 6583 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4393 278
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3926 1937
Size: 1989 4594
Acc65I G'GTAC_C
Cuts at: 3705 3904 3705
Size: 199 6384
AclI AA'CG_TT
Cuts at: 1563 4710 5083 1563
Size: 3147 373 3063
Fragments arranged by size:
3147 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2912 3182 4646 6030 6298 1632
Size: 1280 270 1464 1384 268 1917
Fragments arranged by size:
1917 1464 1384 1280 270 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6386
AflIII A'CryG_T
Cuts at: 1203 5482 6459 1203
Size: 4279 977 1327
Fragments arranged by size:
4279 1327 977
AgeI A'CCGG_T
Cuts at: 5414 5414
Size: 6583
AhdI GACnn_n'nnGTC
Cuts at: 5312 5312
Size: 6583
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6583
AlwNI CAG_nnn'CTG
Cuts at: 2276 2908 3532 4326 5898 2276
Size: 632 624 794 1572 2961
Fragments arranged by size:
2961 1572 794 632 624
ApaLI G'TGCA_C
Cuts at: 4640 5796 4640
Size: 1156 5427
ApoI r'AATT_y
Cuts at: 1493 3892 4118 4270 6379 1493
Size: 2399 226 152 2109 1697
Fragments arranged by size:
2399 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5137 160
Size: 872 4105 1606
Fragments arranged by size:
4105 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
AvaI C'yCGr_G
Cuts at: 1870 1943 2589 3908 1870
Size: 73 646 1319 4545
Fragments arranged by size:
4545 1319 646 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3697 3730 3697
Size: 33 6550
BamHI G'GATC_C
Cuts at: 3913 4280 3913
Size: 367 6216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3705 3904 5359 618
Size: 325 217 627 1918 199 1455 1842
Fragments arranged by size:
1918 1842 1455 627 325 217 199
BanII G_rGCy'C
Cuts at: 1391 2162 3902 1391
Size: 771 1740 4072
Fragments arranged by size:
4072 1740 771
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2922 3302 3318 928
Size: 971 1023 380 16 4193
Fragments arranged by size:
4193 1023 971 380 16
BbvCI CC'TCA_GC
Cuts at: 2056 2056
Size: 6583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4774 4808 1903
Size: 34 2837 34 3678
Fragments arranged by size:
3678 2837 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4475 5685 4475
Size: 1210 5373
BclI T'GATC_A
Cuts at: 1548 2278 1548
Size: 730 5853
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2267 5194 136
Size: 107 122 71 631 605 595 2927 1525
Fragments arranged by size:
2927 1525 631 605 595 122 107 71
BglII A'GATC_T
Cuts at: 6578 6578
Size: 6583
BlpI GC'TnA_GC
Cuts at: 3381 4000 3381
Size: 619 5964
Bme1580I G_kGCm'C
Cuts at: 1790 3453 4644 5800 1790
Size: 1663 1191 1156 2573
Fragments arranged by size:
2573 1663 1191 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6583
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2977 3400 3772
Size: 416 53 83 399 24 1560 423 372
Cuts at: 3772 4314 5272 442
Size: 542 958 1753
Fragments arranged by size:
1753 1560 958 542 423 416 399 372
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6583
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 6583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2603 2806 3196 5243 1239
Size: 1010 354 203 390 2047 2579
Fragments arranged by size:
2579 2047 1010 390 354 203
Bpu10I CC'TnA_GC
Cuts at: 2056 2056
Size: 6583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4708 5573 5871 4708
Size: 865 298 5420
Fragments arranged by size:
5420 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3716 5246 882
Size: 617 2217 1530 2219
Fragments arranged by size:
2219 2217 1530 617
BsaAI yAC'GTr
Cuts at: 493 1204 4374 493
Size: 711 3170 2702
Fragments arranged by size:
3170 2702 711
BsaBI GATnn'nnATC
Cuts at: 2261 6577 2261
Size: 4316 2267
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4390 4772 5407 275
Size: 53 83 186 936 2857 382 635 1451
Fragments arranged by size:
2857 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3075 5016 5414 5688 5835 6246 3075
Size: 1941 398 274 147 411 3412
Fragments arranged by size:
3412 1941 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6358
Fragments arranged by size:
6358 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2044 2138 1186
Size: 853 5 94 5631
Fragments arranged by size:
5631 853 94 5
BseYI C'CCAG_C
Cuts at: 5786 6282 5786
Size: 496 6087
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2960 1163
Size: 573 1224 4786
Fragments arranged by size:
4786 1224 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4033 4794 5822 1540
Size: 275 186 2032 761 1028 2301
Fragments arranged by size:
2301 2032 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 2868 3526 4644 4729 5800 2172
Size: 696 658 1118 85 1071 2955
Fragments arranged by size:
2955 1118 1071 696 658 85
BsmI GAATG_Cn'
Cuts at: 4094 4187 4094
Size: 93 6490
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2172 2868 3453 3526 3902 4644
Size: 399 372 10 696 585 73 376 742
Cuts at: 4644 4729 5800 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 696 585 399 376 372
85 73 10
BspEI T'CCGG_A
Cuts at: 6246 6246
Size: 6583
BspHI T'CATG_A
Cuts at: 3039 4472 6202 3039
Size: 1433 1730 3420
Fragments arranged by size:
3420 1730 1433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2886 3938 844
Size: 2042 1052 3489
Fragments arranged by size:
3489 2042 1052
BsrBI CCG'CTC
Cuts at: 1394 1823 2101 2233 4470 1394
Size: 429 278 132 2237 3507
Fragments arranged by size:
3507 2237 429 278 132
BsrDI GCAATG_nn'
Cuts at: 65 1248 2958 3820 5078 5252 65
Size: 1183 1710 862 1258 174 1396
Fragments arranged by size:
1710 1396 1258 1183 862 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2128 5227 5414 1700
Size: 110 318 3099 187 2869
Fragments arranged by size:
3099 2869 318 187 110
BsrGI T'GTAC_A
Cuts at: 96 2329 3591 96
Size: 2233 1262 3088
Fragments arranged by size:
3088 2233 1262
BssHII G'CGCG_C
Cuts at: 1925 2012 2084 1925
Size: 87 72 6424
Fragments arranged by size:
6424 87 72
BssSI C'ACGA_G
Cuts at: 2171 4643 5655 2171
Size: 2472 1012 3099
Fragments arranged by size:
3099 2472 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2206 794
Size: 1412 5171
BstBI TT'CG_AA
Cuts at: 2461 5387 2461
Size: 2926 3657
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6583
BstXI CCAn_nnnn'nTGG
Cuts at: 2409 2409
Size: 6583
BstYI r'GATC_y
Cuts at: 1972 3199 3913 4280 4665 4682 6134 6220 6232
Size: 1227 714 367 385 17 1452 86 12
Cuts at: 6232 6578 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1227 714 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2628 2732 2628
Size: 104 6479
BtgI C'CryG_G
Cuts at: 513 1065 2004 2163 2703 513
Size: 552 939 159 540 4393
Fragments arranged by size:
4393 939 552 540 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5844
Fragments arranged by size:
5844 683 56
BtsI GCAGTG_nn'
Cuts at: 2906 3453 4099 4893 4913 2906
Size: 547 646 794 20 4576
Fragments arranged by size:
4576 794 646 547 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6548
DraI TTT'AAA
Cuts at: 3887 3887
Size: 6583
DraIII CAC_nnn'GTG
Cuts at: 2287 2350 2287
Size: 63 6520
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2807 5590 809
Size: 507 1491 2783 1802
Fragments arranged by size:
2783 1802 1491 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2411 3753
Size: 54 1425 50 275 18 429 152 1342
Cuts at: 3753 4030 4919 6548 8
Size: 277 889 1629 43
Fragments arranged by size:
1629 1425 1342 889 429 277 275 152
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4030 1537
Size: 275 2218 4090
Fragments arranged by size:
4090 2218 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2672 3410 4513 1353
Size: 1319 738 1103 3423
Fragments arranged by size:
3423 1319 1103 738
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5170 5544 5690 387
Size: 1064 138 3581 374 146 1280
Fragments arranged by size:
3581 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2603 2806 2912 3182 3196 4646
Size: 393 617 354 203 106 270 14 1450
Cuts at: 4646 5243 6030 6298 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1450 787 617 597 393 354 270
268 203 106 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2735 2735
Size: 6583
EcoO109I rG'GnC_Cy
Cuts at: 2593 2646 3640 3832 2593
Size: 53 994 192 5344
Fragments arranged by size:
5344 994 192 53
EcoRI G'AATT_C
Cuts at: 3892 4270 3892
Size: 378 6205
FspI TGC'GCA
Cuts at: 3086 5089 3086
Size: 2003 4580
HaeII r_GCGC'y
Cuts at: 1678 5730 1678
Size: 4052 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2326 2358 2524 2556 2947
Size: 32 135 32 754 32 166 32 391
Cuts at: 2947 2979 5224 5256 5298 5330 1373
Size: 32 2245 32 42 32 2626
Fragments arranged by size:
2626 2245 754 391 166 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2509 3927 4173 669
Size: 988 281 571 1418 246 3079
Fragments arranged by size:
3079 1418 988 571 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6583
HpaI GTT'AAC
Cuts at: 4173 4173
Size: 6583
KpnI G_GTAC'C
Cuts at: 3709 3908 3709
Size: 199 6384
MfeI C'AATT_G
Cuts at: 4182 4182
Size: 6583
MluI A'CGCG_T
Cuts at: 6459 6459
Size: 6583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3517 5697 5881 1305
Size: 2212 2180 184 2007
Fragments arranged by size:
2212 2180 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 2413 3755 10
Size: 54 1425 343 429 152 1342 2838
Fragments arranged by size:
2838 1425 1342 429 343 152 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2843 2936 4541 4900 5059 518
Size: 648 1677 93 1605 359 159 2042
Fragments arranged by size:
2042 1677 1605 648 359 159 93
MspA1I CmG'CkG
Cuts at: 1606 3160 3385 3999 4676 5824 6069 1606
Size: 1554 225 614 677 1148 245 2120
Fragments arranged by size:
2120 1554 1148 677 614 245 225
NcoI C'CATG_G
Cuts at: 513 1065 2004 2703 513
Size: 552 939 699 4393
Fragments arranged by size:
4393 939 699 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6583
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6583
NotI GC'GGCC_GC
Cuts at: 4030 4030
Size: 6583
NspI r_CATG'y
Cuts at: 1132 1229 3779 3941 5486 1132
Size: 97 2550 162 1545 2229
Fragments arranged by size:
2550 2229 1545 162 97
PasI CC'CwG_GG
Cuts at: 1348 3496 1348
Size: 2148 4435
PciI A'CATG_T
Cuts at: 5482 5482
Size: 6583
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3735 3762 1108
Size: 2627 27 3929
Fragments arranged by size:
3929 2627 27
PfoI T'CCnGG_A
Cuts at: 2139 3788 2139
Size: 1649 4934
PmeI GTTT'AAAC
Cuts at: 3887 3887
Size: 6583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3654 3686 4652 4684 6165 6197 3654
Size: 32 966 32 1481 32 4040
Fragments arranged by size:
4040 1481 966 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2646 3640 2646
Size: 994 5589
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6583
PsiI TTA'TAA
Cuts at: 4153 4153
Size: 6583
PstI C_TGCA'G
Cuts at: 830 2157 2913 3661 3935 830
Size: 1327 756 748 274 3478
Fragments arranged by size:
3478 1327 756 748 274
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6583
PvuII CAG'CTG
Cuts at: 3160 3385 3160
Size: 225 6358
SalI G'TCGA_C
Cuts at: 1936 3925 1936
Size: 1989 4594
SbfI CC_TGCA'GG
Cuts at: 3935 3935
Size: 6583
ScaI AGT'ACT
Cuts at: 4831 6543 4831
Size: 1712 4871
SexAI A'CCwGG_T
Cuts at: 3465 3465
Size: 6583
SfcI C'TryA_G
Cuts at: 826 1045 2153 2909 3657 3808 3931 5066 5747
Size: 219 1108 756 748 151 123 1135 681
Cuts at: 5747 5938 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1108 756 748 681 219 191
151 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6583
SmaI CCC'GGG
Cuts at: 1872 3910 1872
Size: 2038 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4687 5588 5850 820
Size: 197 926 2744 901 262 1553
Fragments arranged by size:
2744 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4374 493
Size: 3881 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6583
SphI G_CATG'C
Cuts at: 1132 3941 1132
Size: 2809 3774
SspI AAT'ATT
Cuts at: 5 52 2307 3175 4507 5
Size: 47 2255 868 1332 2081
Fragments arranged by size:
2255 2081 1332 868 47
StuI AGG'CCT
Cuts at: 1517 2362 2744 3217 1517
Size: 845 382 473 4883
Fragments arranged by size:
4883 845 473 382
StyI C'CwwG_G
Cuts at: 513 1065 2004 2703 4022 513
Size: 552 939 699 1319 3074
Fragments arranged by size:
3074 1319 939 699 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4610 4627 4780 4965 5410 325
Size: 1255 3030 17 153 185 445 1498
Fragments arranged by size:
3030 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2329 3591 3615
Size: 274 80 33 51 1443 352 1262 24
Cuts at: 3615 4829 6541 96
Size: 1214 1712 138
Fragments arranged by size:
1712 1443 1262 1214 352 274 138 80
51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6543 6575 6543
Size: 32 6551
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6583
XbaI T'CTAG_A
Cuts at: 3919 3919
Size: 6583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2170 2254 2170
Size: 84 6499
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6583
XmaI C'CCGG_G
Cuts at: 1870 3908 1870
Size: 2038 4545
XmnI GAAnn'nnTTC
Cuts at: 3650 4712 3650
Size: 1062 5521
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4391 276
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI