Restriction map for
pFN21AA0581
(Circular) (Six-base) MAPSORT of: pFN21AA0581.seq Check: 1323 from: 1 to: 8353
pFN21AA0581 8353 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4684 5422 4684
Size: 738 7615
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6163 278
Size: 53 83 186 936 4627 2468
Fragments arranged by size:
4627 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4377 5696 1937
Size: 2440 1319 4594
Fragments arranged by size:
4594 2440 1319
Acc65I G'GTAC_C
Cuts at: 5674 5674
Size: 8353
AclI AA'CG_TT
Cuts at: 1563 6480 6853 1563
Size: 4917 373 3063
Fragments arranged by size:
4917 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4023 6416 7800 8068 1632
Size: 2391 2393 1384 268 1917
Fragments arranged by size:
2393 2391 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2480 2756 820
Size: 197 1463 276 6417
Fragments arranged by size:
6417 1463 276 197
AflIII A'CryG_T
Cuts at: 1203 2439 2955 3809 3811 7252 8229 1203
Size: 1236 516 854 2 3441 977 1327
Fragments arranged by size:
3441 1327 1236 977 854 516 2
AgeI A'CCGG_T
Cuts at: 7184 7184
Size: 8353
AhdI GACnn_n'nnGTC
Cuts at: 7082 7082
Size: 8353
AleI CACnn'nnGTG
Cuts at: 1166 2318 1166
Size: 1152 7201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4503 1184
Size: 3319 5034
AlwNI CAG_nnn'CTG
Cuts at: 4354 4664 5050 5410 5573 6096 7668 4354
Size: 310 386 360 163 523 1572 5039
Fragments arranged by size:
5039 1572 523 386 360 310 163
ApaLI G'TGCA_C
Cuts at: 2027 6410 7566 2027
Size: 4383 1156 2814
Fragments arranged by size:
4383 2814 1156
ApoI r'AATT_y
Cuts at: 1493 2084 2696 3387 3672 3758 4268 4866 5462
Size: 591 612 691 285 86 510 598 596
Cuts at: 5462 5492 5636 5662 5888 6040 8149 1493
Size: 30 144 26 226 152 2109 1697
Fragments arranged by size:
2109 1697 691 612 598 596 591 510
285 226 152 144 86 30 26
AseI AT'TA_AT
Cuts at: 160 1032 2985 6907 160
Size: 872 1953 3922 1606
Fragments arranged by size:
3922 1953 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8353
AvaI C'yCGr_G
Cuts at: 1870 1943 5480 5678 1870
Size: 73 3537 198 4545
Fragments arranged by size:
4545 3537 198 73
BamHI G'GATC_C
Cuts at: 5683 6050 5683
Size: 367 7986
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2292 4672 5674 7129
Size: 325 217 627 291 214 2380 1002 1455
Cuts at: 7129 618
Size: 1842
Fragments arranged by size:
2380 1842 1455 1002 627 325 291 217
214
BanII G_rGCy'C
Cuts at: 1391 2017 3324 3501 5672 1391
Size: 626 1307 177 2171 4072
Fragments arranged by size:
4072 2171 1307 626 177
BbeI G_GCGC'C
Cuts at: 2296 2296
Size: 8353
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2576 2958 3699 5294 5535 928
Size: 971 677 382 741 1595 241 3746
Fragments arranged by size:
3746 1595 971 741 677 382 241
BbvCI CC'TCA_GC
Cuts at: 3837 3837
Size: 8353
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2068 2102 4286 4320 6544 6578 1903
Size: 34 131 34 2184 34 2224 34 3678
Fragments arranged by size:
3678 2224 2184 131 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4117 6245 7455 4117
Size: 2128 1210 5015
Fragments arranged by size:
5015 2128 1210
BclI T'GATC_A
Cuts at: 1548 2310 2731 4954 1548
Size: 762 421 2223 4947
Fragments arranged by size:
4947 2223 762 421
BfrBI ATG'CAT
Cuts at: 2453 2453
Size: 8353
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3275 6964 136
Size: 107 122 71 631 605 1603 3689 1525
Fragments arranged by size:
3689 1603 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4690 8348 4690
Size: 3658 4695
BlpI GC'TnA_GC
Cuts at: 3785 4476 5770 3785
Size: 691 1294 6368
Fragments arranged by size:
6368 1294 691
Bme1580I G_kGCm'C
Cuts at: 1790 2031 2081 6414 7570 1790
Size: 241 50 4333 1156 2573
Fragments arranged by size:
4333 2573 1156 241 50
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8353
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6084 7042 442
Size: 416 53 83 399 24 4667 958 1753
Fragments arranged by size:
4667 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8353
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2666 4593 2666
Size: 1927 6426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2318 2481 2613 3369 3469 3735 4557 4563
Size: 1079 163 132 756 100 266 822 6
Cuts at: 4563 4641 5462 5584 7013 1239
Size: 78 821 122 1429 2579
Fragments arranged by size:
2579 1429 1079 822 821 756 266 163
132 122 100 78 6
Bpu10I CC'TnA_GC
Cuts at: 3837 3837
Size: 8353
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 2054 2598 4903 6478 7343 7641 2027
Size: 27 544 2305 1575 865 298 2739
Fragments arranged by size:
2739 2305 1575 865 544 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7016 882
Size: 617 5517 2219
Fragments arranged by size:
5517 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2264 3812 4093 6144 493
Size: 711 1060 1548 281 2051 2702
Fragments arranged by size:
2702 2051 1548 1060 711 281
BsaBI GATnn'nnATC
Cuts at: 8347 8347
Size: 8353
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2293 6160 6542 7177
Size: 53 83 186 936 760 3867 382 635
Cuts at: 7177 275
Size: 1451
Fragments arranged by size:
3867 1451 936 760 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5301 6786 7184 7458 7605 8016 5301
Size: 1485 398 274 147 411 5638
Fragments arranged by size:
5638 1485 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3464 3494 3733 3763 1178
Size: 30 165 30 2061 30 239 30 5768
Fragments arranged by size:
5768 2061 239 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3025 3472 3985 5297 5621 5631 5637 1186
Size: 1839 447 513 1312 324 10 6 3902
Fragments arranged by size:
3902 1839 1312 513 447 324 10 6
BseYI C'CCAG_C
Cuts at: 4943 5611 7556 8052 4943
Size: 668 1945 496 5244
Fragments arranged by size:
5244 1945 668 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5345 5519 1163
Size: 573 3609 174 3997
Fragments arranged by size:
3997 3609 573 174
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5803 6564 7592 1540
Size: 275 186 3802 761 1028 2301
Fragments arranged by size:
3802 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2031 3063 6414 6499 7570 2031
Size: 1032 3351 85 1071 2814
Fragments arranged by size:
3351 2814 1071 1032 85
BsmI GAATG_Cn'
Cuts at: 3856 5864 5957 3856
Size: 2008 93 6252
Fragments arranged by size:
6252 2008 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2031 2081 3063 3324 3501 5672
Size: 399 227 14 50 982 261 177 2171
Cuts at: 5672 6414 6499 7570 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 2171 1071 982 742 399 261 227
177 85 50 14
BspEI T'CCGG_A
Cuts at: 8016 8016
Size: 8353
BspHI T'CATG_A
Cuts at: 3966 6242 7972 3966
Size: 2276 1730 4347
Fragments arranged by size:
4347 2276 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2433 3845 4684 5422 5708 844
Size: 1589 1412 839 738 286 3489
Fragments arranged by size:
3489 1589 1412 839 738 286
BsrBI CCG'CTC
Cuts at: 1394 1823 6240 1394
Size: 429 4417 3507
Fragments arranged by size:
4417 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6848 7022 65
Size: 1183 5600 174 1396
Fragments arranged by size:
5600 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6997 7184 1700
Size: 110 5187 187 2869
Fragments arranged by size:
5187 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2637 96
Size: 2541 5812
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8353
BssSI C'ACGA_G
Cuts at: 2336 2360 6413 7425 2336
Size: 24 4053 1012 3264
Fragments arranged by size:
4053 3264 1012 24
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2035 5050 794
Size: 1241 3015 4097
Fragments arranged by size:
4097 3015 1241
BstBI TT'CG_AA
Cuts at: 7157 7157
Size: 8353
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8353
BstXI CCAn_nnnn'nTGG
Cuts at: 3026 3026
Size: 8353
BstYI r'GATC_y
Cuts at: 1972 2197 2767 4690 5406 5683 6050 6435 6452
Size: 225 570 1923 716 277 367 385 17
Cuts at: 6452 7904 7990 8002 8348 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1923 1452 716 570 385 367 346
277 225 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2145 2145
Size: 8353
BtgI C'CryG_G
Cuts at: 513 1065 2004 3136 513
Size: 552 939 1132 5730
Fragments arranged by size:
5730 1132 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7614
Fragments arranged by size:
7614 683 56
BtsI GCAGTG_nn'
Cuts at: 5869 6663 6683 5869
Size: 794 20 7539
Fragments arranged by size:
7539 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8353
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8318
DraI TTT'AAA
Cuts at: 5657 5657
Size: 8353
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7360 809
Size: 507 6044 1802
Fragments arranged by size:
6044 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2382 3016
Size: 54 1425 50 275 18 177 375 634
Cuts at: 3016 3609 4276 4307 5800 6689 8318 8
Size: 593 667 31 1493 889 1629 43
Fragments arranged by size:
1629 1493 1425 889 667 634 593 375
275 177 54 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 5800 1537
Size: 275 3988 4090
Fragments arranged by size:
4090 3988 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3111 3363 3848 3932 5139 5274 6283 1353
Size: 1758 252 485 84 1207 135 1009 3423
Fragments arranged by size:
3423 1758 1207 1009 485 252 135 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3293 4900 6940 7314 7460 387
Size: 1064 138 1704 1607 2040 374 146 1280
Fragments arranged by size:
2040 1704 1607 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2318 2481 2613 3369 3469 3735 4023
Size: 393 686 163 132 756 100 266 288
Cuts at: 4023 4557 4563 4641 5462 5584 6416 7013 7800
Size: 534 6 78 821 122 832 597 787
Cuts at: 7800 8068 1239
Size: 268 1524
Fragments arranged by size:
1524 832 821 787 756 686 597 534
393 288 268 266 163 132 122 100
78 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2072 2072
Size: 8353
EcoO109I rG'GnC_Cy
Cuts at: 2251 2251
Size: 8353
EcoRI G'AATT_C
Cuts at: 5492 5662 6040 5492
Size: 170 378 7805
Fragments arranged by size:
7805 378 170
FspI TGC'GCA
Cuts at: 6859 6859
Size: 8353
HaeII r_GCGC'y
Cuts at: 1678 2296 7500 1678
Size: 618 5204 2531
Fragments arranged by size:
5204 2531 618
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2128 2160 3488
Size: 32 135 32 518 32 6 32 1328
Cuts at: 3488 3520 5463 5495 5607 5639 6994 7026 7068
Size: 32 1943 32 112 32 1355 32 42
Cuts at: 7068 7100 1373
Size: 32 2626
Fragments arranged by size:
2626 1943 1355 1328 518 135 112 42
32 32 32 32 32 32 32 32
32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 5130 5697 5943 669
Size: 988 281 3192 567 246 3079
Fragments arranged by size:
3192 3079 988 567 281 246
HindIII A'AGCT_T
Cuts at: 748 4428 748
Size: 3680 4673
HpaI GTT'AAC
Cuts at: 5943 5943
Size: 8353
KasI G'GCGC_C
Cuts at: 2292 2292
Size: 8353
KpnI G_GTAC'C
Cuts at: 5678 5678
Size: 8353
MfeI C'AATT_G
Cuts at: 3186 4481 5952 3186
Size: 1295 1471 5587
Fragments arranged by size:
5587 1471 1295
MluI A'CGCG_T
Cuts at: 8229 8229
Size: 8353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 3489 3849 7467 7651 1305
Size: 780 1404 360 3618 184 2007
Fragments arranged by size:
3618 2007 1404 780 360 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 3018 3611 4278 10
Size: 54 1425 343 552 634 593 667 4085
Fragments arranged by size:
4085 1425 667 634 593 552 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2318 4385 6311 6670 6829 518
Size: 648 1152 2067 1926 359 159 2042
Fragments arranged by size:
2067 2042 1926 1152 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3014 5050 5769 6446 7594 7839 1606
Size: 1408 2036 719 677 1148 245 2120
Fragments arranged by size:
2120 2036 1408 1148 719 677 245
NarI GG'CG_CC
Cuts at: 2293 2293
Size: 8353
NcoI C'CATG_G
Cuts at: 513 1065 2004 3136 513
Size: 552 939 1132 5730
Fragments arranged by size:
5730 1132 939 552
NdeI CA'TA_TG
Cuts at: 387 4411 387
Size: 4024 4329
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8353
NotI GC'GGCC_GC
Cuts at: 5800 5800
Size: 8353
NsiI A_TGCA'T
Cuts at: 2455 2455
Size: 8353
NspI r_CATG'y
Cuts at: 1132 1229 2443 2959 3493 5711 7256 1132
Size: 97 1214 516 534 2218 1545 2229
Fragments arranged by size:
2229 2218 1545 1214 534 516 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8353
PciI A'CATG_T
Cuts at: 2439 2955 7252 2439
Size: 516 4297 3540
Fragments arranged by size:
4297 3540 516
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2391 3889 5320 1108
Size: 1283 1498 1431 4141
Fragments arranged by size:
4141 1498 1431 1283
PfoI T'CCnGG_A
Cuts at: 4534 5313 5409 4534
Size: 779 96 7478
Fragments arranged by size:
7478 779 96
PmeI GTTT'AAAC
Cuts at: 5657 5657
Size: 8353
PmlI CAC'GTG
Cuts at: 3812 3812
Size: 8353
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6422 6454 7935 7967 6422
Size: 32 1481 32 6808
Fragments arranged by size:
6808 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2251 2251
Size: 8353
PshAI GACnn'nnGTC
Cuts at: 1613 5135 5268 1613
Size: 3522 133 4698
Fragments arranged by size:
4698 3522 133
PsiI TTA'TAA
Cuts at: 5923 5923
Size: 8353
PstI C_TGCA'G
Cuts at: 830 3115 5433 5705 830
Size: 2285 2318 272 3478
Fragments arranged by size:
3478 2318 2285 272
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8353
PvuII CAG'CTG
Cuts at: 3014 5050 3014
Size: 2036 6317
SalI G'TCGA_C
Cuts at: 1936 5695 1936
Size: 3759 4594
SapI GCTCTTCn'nnn_
Cuts at: 3848 3848
Size: 8353
SbfI CC_TGCA'GG
Cuts at: 5705 5705
Size: 8353
ScaI AGT'ACT
Cuts at: 3284 6601 8313 3284
Size: 3317 1712 3324
Fragments arranged by size:
3324 3317 1712
SexAI A'CCwGG_T
Cuts at: 4800 4800
Size: 8353
SfcI C'TryA_G
Cuts at: 826 1045 3111 3472 3946 5429 5701 6836 7517
Size: 219 2066 361 474 1483 272 1135 681
Cuts at: 7517 7708 826
Size: 191 1471
Fragments arranged by size:
2066 1483 1471 1135 681 474 361 272
219 191
SfoI GGC'GCC
Cuts at: 2294 2294
Size: 8353
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8353
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8353
SmaI CCC'GGG
Cuts at: 1872 5680 1872
Size: 3808 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2042 2069 2480 2613 2756 4882
Size: 197 926 99 27 411 133 143 2126
Cuts at: 4882 5480 6457 7358 7620 820
Size: 598 977 901 262 1553
Fragments arranged by size:
2126 1553 977 926 901 598 411 262
197 143 133 99 27
SnaBI TAC'GTA
Cuts at: 493 6144 493
Size: 5651 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8353
SphI G_CATG'C
Cuts at: 1132 5711 1132
Size: 4579 3774
SspI AAT'ATT
Cuts at: 5 52 6277 5
Size: 47 6225 2081
Fragments arranged by size:
6225 2081 47
StuI AGG'CCT
Cuts at: 1517 3976 1517
Size: 2459 5894
StyI C'CwwG_G
Cuts at: 513 1065 2004 2247 2385 3136 4159 5792 513
Size: 552 939 243 138 751 1023 1633 3074
Fragments arranged by size:
3074 1633 1023 939 751 552 243 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3149 4633 6380 6397 6550 6735 7180
Size: 1255 1569 1484 1747 17 153 185 445
Cuts at: 7180 325
Size: 1498
Fragments arranged by size:
1747 1569 1498 1484 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2637 3282 6599
Size: 274 80 33 51 1443 660 645 3317
Cuts at: 6599 8311 96
Size: 1712 138
Fragments arranged by size:
3317 1712 1443 660 645 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3733 3765 3798 3830 3864 3896 4155 4187 8313
Size: 32 33 32 34 32 259 32 4126
Cuts at: 8313 8345 3733
Size: 32 3741
Fragments arranged by size:
4126 3741 259 34 33 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8353
XbaI T'CTAG_A
Cuts at: 5689 5689
Size: 8353
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3751 5431 5608 3751
Size: 1680 177 6496
Fragments arranged by size:
6496 1680 177
XhoI C'TCGA_G
Cuts at: 1943 5480 1943
Size: 3537 4816
XmaI C'CCGG_G
Cuts at: 1870 5678 1870
Size: 3808 4545
XmnI GAAnn'nnTTC
Cuts at: 6482 6482
Size: 8353
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6161 276
Size: 53 83 186 936 4627 2468
Fragments arranged by size:
4627 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SfiI SrfI SwaI