Restriction map for
pFN21AA0577
(Circular) (Six-base) MAPSORT of: pFN21AA0577.seq Check: 7355 from: 1 to: 7828
pFN21AA0577 7828 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5638 278
Size: 53 83 186 936 4102 2468
Fragments arranged by size:
4102 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4217 5171 1937
Size: 2280 954 4594
Fragments arranged by size:
4594 2280 954
Acc65I G'GTAC_C
Cuts at: 2099 2212 5149 2099
Size: 113 2937 4778
Fragments arranged by size:
4778 2937 113
AclI AA'CG_TT
Cuts at: 1563 5955 6328 1563
Size: 4392 373 3063
Fragments arranged by size:
4392 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2504 3041 3642 5891 7275 7543 1632
Size: 872 537 601 2249 1384 268 1917
Fragments arranged by size:
2249 1917 1384 872 601 537 268
AfeI AGC'GCT
Cuts at: 2030 2111 2030
Size: 81 7747
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7631
AflIII A'CryG_T
Cuts at: 1203 4774 6727 7704 1203
Size: 3571 1953 977 1327
Fragments arranged by size:
3571 1953 1327 977
AgeI A'CCGG_T
Cuts at: 6659 6659
Size: 7828
AhdI GACnn_n'nnGTC
Cuts at: 6557 6557
Size: 7828
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7828
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2128 4219 4410 1184
Size: 944 2091 191 4602
Fragments arranged by size:
4602 2091 944 191
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4067 4099 4067
Size: 32 7796
AlwNI CAG_nnn'CTG
Cuts at: 2092 2527 3770 4262 4535 4797 5005 5571 7143
Size: 435 1243 492 273 262 208 566 1572
Cuts at: 7143 2092
Size: 2777
Fragments arranged by size:
2777 1572 1243 566 492 435 273 262
208
ApaI G_GGCC'C
Cuts at: 2272 3034 2272
Size: 762 7066
ApaLI G'TGCA_C
Cuts at: 5885 7041 5885
Size: 1156 6672
ApoI r'AATT_y
Cuts at: 1493 5137 5363 5515 7624 1493
Size: 3644 226 152 2109 1697
Fragments arranged by size:
3644 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6382 160
Size: 872 5350 1606
Fragments arranged by size:
5350 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7828
AvaI C'yCGr_G
Cuts at: 1870 1943 2181 2244 2265 2844 2916 2931 3033
Size: 73 238 63 21 579 72 15 102
Cuts at: 3033 3393 3402 4737 5153 1870
Size: 360 9 1335 416 4545
Fragments arranged by size:
4545 1335 579 416 360 238 102 73
72 63 21 15 9
BamHI G'GATC_C
Cuts at: 4075 4317 5158 5525 4075
Size: 242 841 367 6378
Fragments arranged by size:
6378 841 367 242
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2099 2212 5149 6604 618
Size: 325 217 627 312 113 2937 1455 1842
Fragments arranged by size:
2937 1842 1455 627 325 312 217 113
BanII G_rGCy'C
Cuts at: 1391 2272 2814 2968 3034 3057 3228 3642 3914
Size: 881 542 154 66 23 171 414 272
Cuts at: 3914 3930 4408 4428 4738 4767 5147 1391
Size: 16 478 20 310 29 380 4072
Fragments arranged by size:
4072 881 542 478 414 380 310 272
171 154 66 29 23 20 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2313 2661 3293 4900 928
Size: 971 414 348 632 1607 3856
Fragments arranged by size:
3856 1607 971 632 414 348
BbvCI CC'TCA_GC
Cuts at: 4156 4156
Size: 7828
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6019 6053 1903
Size: 34 4082 34 3678
Fragments arranged by size:
4082 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5720 6930 5720
Size: 1210 6618
BclI T'GATC_A
Cuts at: 1548 3609 1548
Size: 2061 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3398 3909 4951
Size: 107 122 71 631 605 1726 511 1042
Cuts at: 4951 6439 136
Size: 1488 1525
Fragments arranged by size:
1726 1525 1488 1042 631 605 511 122
107 71
BglII A'GATC_T
Cuts at: 7823 7823
Size: 7828
BlpI GC'TnA_GC
Cuts at: 2066 2550 2712 2789 5245 2066
Size: 484 162 77 2456 4649
Fragments arranged by size:
4649 2456 484 162 77
Bme1580I G_kGCm'C
Cuts at: 1790 2272 3034 4006 5889 7045 1790
Size: 482 762 972 1883 1156 2573
Fragments arranged by size:
2573 1883 1156 972 762 482
BmgBI CAC'GTC
Cuts at: 1318 2080 2782 4434 1318
Size: 762 702 1652 4712
Fragments arranged by size:
4712 1652 762 702
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2080 4684 5559
Size: 416 53 83 399 24 663 2604 875
Cuts at: 5559 6517 442
Size: 958 1753
Fragments arranged by size:
2604 1753 958 875 663 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7828
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 3242 3295 3538 2068
Size: 1174 53 243 6358
Fragments arranged by size:
6358 1174 243 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2297 2342 2549 2762 2900 3095 3101
Size: 806 252 45 207 213 138 195 6
Cuts at: 3101 3747 3872 5027 5051 6488 1239
Size: 646 125 1155 24 1437 2579
Fragments arranged by size:
2579 1437 1155 806 646 252 213 207
195 138 125 45 24 6
Bpu10I CC'TnA_GC
Cuts at: 2676 3280 3636 4156 4312 2676
Size: 604 356 520 156 6192
Fragments arranged by size:
6192 604 520 356 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2624 2753 3626 4265 4289 5953 6818 7116 2624
Size: 129 873 639 24 1664 865 298 3336
Fragments arranged by size:
3336 1664 873 865 639 298 129 24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3080 4793 6491 882
Size: 617 1581 1713 1698 2219
Fragments arranged by size:
2219 1713 1698 1581 617
BsaAI yAC'GTr
Cuts at: 493 1204 5619 493
Size: 711 4415 2702
Fragments arranged by size:
4415 2702 711
BsaBI GATnn'nnATC
Cuts at: 7822 7822
Size: 7828
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 3702 5635 6017
Size: 53 83 186 936 479 1690 1933 382
Cuts at: 6017 6652 275
Size: 635 1451
Fragments arranged by size:
1933 1690 1451 936 635 479 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2658 6261 6659 6933 7080 7491 2658
Size: 3603 398 274 147 411 2995
Fragments arranged by size:
3603 2995 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3044 3074 3745 3775 4376
Size: 30 165 30 1641 30 671 30 601
Cuts at: 4376 4406 1178
Size: 30 4600
Fragments arranged by size:
4600 1641 671 601 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2342 2426 2546 2777 2966 2990 3008
Size: 955 201 84 120 231 189 24 18
Cuts at: 3008 3092 3095 3098 3143 3236 3332 3875 4532
Size: 84 3 3 45 93 96 543 657
Cuts at: 4532 4915 1186
Size: 383 4099
Fragments arranged by size:
4099 955 657 543 383 231 201 189
120 96 93 84 84 45 24 18
3 3
BseYI C'CCAG_C
Cuts at: 2031 2861 2988 3162 3819 7031 7527 2031
Size: 830 127 174 657 3212 496 2332
Fragments arranged by size:
3212 2332 830 657 496 174 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 2098 2156 3337 3445 1163
Size: 573 297 65 58 1181 108 5546
Fragments arranged by size:
5546 1181 573 297 108 65 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5278 6039 7067 1540
Size: 275 186 3277 761 1028 2301
Fragments arranged by size:
3277 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2814 3228 3642 3930 4428 5889 5974 7045 2814
Size: 414 414 288 498 1461 85 1071 3597
Fragments arranged by size:
3597 1461 1071 498 414 414 288 85
BsmI GAATG_Cn'
Cuts at: 5339 5432 5339
Size: 93 7735
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2272 2814 2968 3034 3057 3228 3642
Size: 399 482 542 154 66 23 171 414
Cuts at: 3642 3914 3930 4006 4408 4428 4738 4767 5147
Size: 272 16 76 402 20 310 29 380
Cuts at: 5147 5889 5974 7045 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 542 482 414 402 399
380 310 272 171 154 85 76 66
29 23 20 16
BspEI T'CCGG_A
Cuts at: 7491 7491
Size: 7828
BspHI T'CATG_A
Cuts at: 4992 5717 7447 4992
Size: 725 1730 5373
Fragments arranged by size:
5373 1730 725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3720 4172 5183 844
Size: 2876 452 1011 3489
Fragments arranged by size:
3489 2876 1011 452
BsrBI CCG'CTC
Cuts at: 1394 1823 2074 2554 2803 5715 1394
Size: 429 251 480 249 2912 3507
Fragments arranged by size:
3507 2912 480 429 251 249
BsrDI GCAATG_nn'
Cuts at: 65 1248 4029 4294 6323 6497 65
Size: 1183 2781 265 2029 174 1396
Fragments arranged by size:
2781 2029 1396 1183 265 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2176 2793 4459 6472 6659 1700
Size: 110 204 162 617 1666 2013 187 2869
Fragments arranged by size:
2869 2013 1666 617 204 187 162 110
BsrGI T'GTAC_A
Cuts at: 96 4711 96
Size: 4615 3213
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7828
BssSI C'ACGA_G
Cuts at: 2798 4238 5112 5888 6900 2798
Size: 1440 874 776 1012 3726
Fragments arranged by size:
3726 1440 1012 874 776
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7828
BstBI TT'CG_AA
Cuts at: 3715 6632 3715
Size: 2917 4911
BstEII G'GTnAC_C
Cuts at: 1184 4631 1184
Size: 3447 4381
BstXI CCAn_nnnn'nTGG
Cuts at: 3385 3827 3385
Size: 442 7386
BstYI r'GATC_y
Cuts at: 1972 2164 2836 2941 3297 3807 4075 4266 4317
Size: 192 672 105 356 510 268 191 51
Cuts at: 4317 4521 5071 5158 5525 5910 5927 7379 7465
Size: 204 550 87 367 385 17 1452 86
Cuts at: 7465 7477 7823 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 672 550 510 385 367 356
346 268 204 192 191 105 87 86
51 17 12
BstZ17I GTA'TAC
Cuts at: 4218 4218
Size: 7828
Bsu36I CC'TnA_GG
Cuts at: 2387 2387
Size: 7828
BtgI C'CryG_G
Cuts at: 513 1065 2004 2667 513
Size: 552 939 663 5674
Fragments arranged by size:
5674 939 663 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7089
Fragments arranged by size:
7089 683 56
BtsI GCAGTG_nn'
Cuts at: 2232 2329 3672 4367 4833 5344 6138 6158 2232
Size: 97 1343 695 466 511 794 20 3902
Fragments arranged by size:
3902 1343 794 695 511 466 97 20
ClaI AT'CG_AT
Cuts at: 1554 4724 1554
Size: 3170 4658
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3676 3711 529
Size: 35 3112 35 4646
Fragments arranged by size:
4646 3112 35 35
DraI TTT'AAA
Cuts at: 5132 5132
Size: 7828
DraIII CAC_nnn'GTG
Cuts at: 2056 4665 2056
Size: 2609 5219
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4586 6835 809
Size: 507 3270 2249 1802
Fragments arranged by size:
3270 2249 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2646 3387 5275
Size: 54 1425 50 275 18 816 741 1888
Cuts at: 5275 6164 7793 8
Size: 889 1629 43
Fragments arranged by size:
1888 1629 1425 889 816 741 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5275 1537
Size: 275 3463 4090
Fragments arranged by size:
4090 3463 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2310 2403 2412 2616 4091 4690 5112 5758
Size: 957 93 9 204 1475 599 422 646
Cuts at: 5758 1353
Size: 3423
Fragments arranged by size:
3423 1475 957 646 599 422 204 93
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2762 3179 4199 6415 6789 6935
Size: 1064 138 1173 417 1020 2216 374 146
Cuts at: 6935 387
Size: 1280
Fragments arranged by size:
2216 1280 1173 1064 1020 417 374 146
138
EcoICRI GAG'CTC
Cuts at: 2812 3226 3640 3928 4426 2812
Size: 414 414 288 498 6214
Fragments arranged by size:
6214 498 414 414 288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2297 2342 2504 2549 2762 2900
Size: 393 413 252 45 162 45 213 138
Cuts at: 2900 3041 3095 3101 3642 3747 3872 5027 5051
Size: 141 54 6 541 105 125 1155 24
Cuts at: 5051 5891 6488 7275 7543 1239
Size: 840 597 787 268 1524
Fragments arranged by size:
1524 1155 840 787 597 541 413 393
268 252 213 162 141 138 125 105
54 45 45 24 6
EcoO109I rG'GnC_Cy
Cuts at: 2269 2524 2737 3030 3579 3646 2269
Size: 255 213 293 549 67 6451
Fragments arranged by size:
6451 549 293 255 213 67
EcoRI G'AATT_C
Cuts at: 5137 5515 5137
Size: 378 7450
EcoRV GAT'ATC
Cuts at: 3835 3835
Size: 7828
FseI GG_CCGG'CC
Cuts at: 2180 2180
Size: 7828
FspI TGC'GCA
Cuts at: 6334 6334
Size: 7828
HaeII r_GCGC'y
Cuts at: 1678 2032 2113 2143 2638 2989 6975 1678
Size: 354 81 30 495 351 3986 2531
Fragments arranged by size:
3986 2531 495 354 351 81 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2942 2974 3029 3061 3119
Size: 32 135 32 1370 32 55 32 58
Cuts at: 3119 3151 3449 3481 3624 3656 6469 6501 6543
Size: 32 298 32 143 32 2813 32 42
Cuts at: 6543 6575 1373
Size: 32 2626
Fragments arranged by size:
2813 2626 1370 298 143 135 58 55
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4552 4612 4866 5172 5418 669
Size: 988 281 2614 60 254 306 246 3079
Fragments arranged by size:
3079 2614 988 306 281 254 246 60
HindIII A'AGCT_T
Cuts at: 748 2729 3419 3783 748
Size: 1981 690 364 4793
Fragments arranged by size:
4793 1981 690 364
HpaI GTT'AAC
Cuts at: 5418 5418
Size: 7828
KpnI G_GTAC'C
Cuts at: 2103 2216 5153 2103
Size: 113 2937 4778
Fragments arranged by size:
4778 2937 113
MfeI C'AATT_G
Cuts at: 5427 5427
Size: 7828
MluI A'CGCG_T
Cuts at: 7704 7704
Size: 7828
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2463 3655 6942 7126 1305
Size: 1158 1192 3287 184 2007
Fragments arranged by size:
3287 2007 1192 1158 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6006
Fragments arranged by size:
6006 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3383 4067 4118 5786 6145 6304 518
Size: 648 2217 684 51 1668 359 159 2042
Fragments arranged by size:
2217 2042 1668 684 648 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 2566 2861 2983 4390 4509 4756 5244 5921
Size: 960 295 122 1407 119 247 488 677
Cuts at: 5921 7069 7314 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1407 1148 960 677 488 295 247
245 122 119
NaeI GCC'GGC
Cuts at: 2016 2178 2795 2016
Size: 162 617 7049
Fragments arranged by size:
7049 617 162
NcoI C'CATG_G
Cuts at: 513 1065 2004 2667 513
Size: 552 939 663 5674
Fragments arranged by size:
5674 939 663 552
NdeI CA'TA_TG
Cuts at: 387 4360 387
Size: 3973 3855
NgoMIV G'CCGG_C
Cuts at: 2014 2176 2793 2014
Size: 162 617 7049
Fragments arranged by size:
7049 617 162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7828
NotI GC'GGCC_GC
Cuts at: 5275 5275
Size: 7828
NspI r_CATG'y
Cuts at: 1132 1229 2905 3970 5186 6731 1132
Size: 97 1676 1065 1216 1545 2229
Fragments arranged by size:
2229 1676 1545 1216 1065 97
PasI CC'CwG_GG
Cuts at: 1348 2969 3827 1348
Size: 1621 858 5349
Fragments arranged by size:
5349 1621 858
PciI A'CATG_T
Cuts at: 6727 6727
Size: 7828
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7828
PfoI T'CCnGG_A
Cuts at: 2592 3510 3888 3921 4344 2592
Size: 918 378 33 423 6076
Fragments arranged by size:
6076 918 423 378 33
PmeI GTTT'AAAC
Cuts at: 5132 5132
Size: 7828
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5897 5929 7410 7442 5897
Size: 32 1481 32 6283
Fragments arranged by size:
6283 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2524 2737 3579 3646 2524
Size: 213 842 67 6706
Fragments arranged by size:
6706 842 213 67
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7828
PsiI TTA'TAA
Cuts at: 5052 5398 5052
Size: 346 7482
PspOMI G'GGCC_C
Cuts at: 2268 3030 2268
Size: 762 7066
PstI C_TGCA'G
Cuts at: 830 4148 4198 5180 830
Size: 3318 50 982 3478
Fragments arranged by size:
3478 3318 982 50
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7828
PvuII CAG'CTG
Cuts at: 2861 4390 4509 4756 2861
Size: 1529 119 247 5933
Fragments arranged by size:
5933 1529 247 119
SacI G_AGCT'C
Cuts at: 2814 3228 3642 3930 4428 2814
Size: 414 414 288 498 6214
Fragments arranged by size:
6214 498 414 414 288
SalI G'TCGA_C
Cuts at: 1936 5170 1936
Size: 3234 4594
SapI GCTCTTCn'nnn_
Cuts at: 4091 5112 4091
Size: 1021 6807
SbfI CC_TGCA'GG
Cuts at: 5180 5180
Size: 7828
ScaI AGT'ACT
Cuts at: 6076 7788 6076
Size: 1712 6116
SexAI A'CCwGG_T
Cuts at: 4638 4638
Size: 7828
SfcI C'TryA_G
Cuts at: 826 1045 3439 4144 4194 5176 6311 6992 7183
Size: 219 2394 705 50 982 1135 681 191
Cuts at: 7183 826
Size: 1471
Fragments arranged by size:
2394 1471 1135 982 705 681 219 191
50
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7828
SgrAI Cr'CCGG_yG
Cuts at: 1700 2014 1700
Size: 314 7514
SmaI CCC'GGG
Cuts at: 1872 2183 2267 2846 3404 4739 5155 1872
Size: 311 84 579 558 1335 416 4545
Fragments arranged by size:
4545 1335 579 558 416 311 84
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2639 2732 3641 4244 4304 5932
Size: 197 926 696 93 909 603 60 1628
Cuts at: 5932 6833 7095 820
Size: 901 262 1553
Fragments arranged by size:
1628 1553 926 909 901 696 603 262
197 93 60
SnaBI TAC'GTA
Cuts at: 493 5619 493
Size: 5126 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7828
SphI G_CATG'C
Cuts at: 1132 5186 1132
Size: 4054 3774
SrfI GCCC'GGGC
Cuts at: 2267 2267
Size: 7828
SspI AAT'ATT
Cuts at: 5 52 5752 5
Size: 47 5700 2081
Fragments arranged by size:
5700 2081 47
StuI AGG'CCT
Cuts at: 1517 4154 1517
Size: 2637 5191
StyI C'CwwG_G
Cuts at: 513 1065 2004 2160 2667 2907 3756 4417 5058
Size: 552 939 156 507 240 849 661 641
Cuts at: 5058 5267 513
Size: 209 3074
Fragments arranged by size:
3074 939 849 661 641 552 507 240
209 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3160 3308 3371 3401 5855 5872 6025
Size: 1255 1580 148 63 30 2454 17 153
Cuts at: 6025 6210 6655 325
Size: 185 445 1498
Fragments arranged by size:
2454 1580 1498 1255 445 185 153 148
63 30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4503 4711 6074
Size: 274 80 33 51 1443 2526 208 1363
Cuts at: 6074 7786 96
Size: 1712 138
Fragments arranged by size:
2526 1712 1443 1363 274 208 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2383 2415 2477 2494 2509 2526 3745 3777 7788
Size: 32 62 17 15 17 1219 32 4011
Cuts at: 7788 7820 2383
Size: 32 2391
Fragments arranged by size:
4011 2391 1219 62 32 32 32 17
17 15
Tth111I GACn'n_nGTC
Cuts at: 1316 4583 1316
Size: 3267 4561
XbaI T'CTAG_A
Cuts at: 5164 5164
Size: 7828
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3064 3064
Size: 7828
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7828
XmaI C'CCGG_G
Cuts at: 1870 2181 2265 2844 3402 4737 5153 1870
Size: 311 84 579 558 1335 416 4545
Fragments arranged by size:
4545 1335 579 558 416 311 84
XmnI GAAnn'nnTTC
Cuts at: 2517 4205 5957 2517
Size: 1688 1752 4388
Fragments arranged by size:
4388 1752 1688
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5636 276
Size: 53 83 186 936 4102 2468
Fragments arranged by size:
4102 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
EcoNI FalI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SwaI