Restriction map for
pFN21AA0575
(Circular) (Six-base) MAPSORT of: pFN21AA0575.seq Check: 1774 from: 1 to: 7546
pFN21AA0575 7546 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5356 278
Size: 53 83 186 936 3820 2468
Fragments arranged by size:
3820 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4227 4518 4889 1937
Size: 2290 291 371 4594
Fragments arranged by size:
4594 2290 371 291
Acc65I G'GTAC_C
Cuts at: 4867 4867
Size: 7546
AclI AA'CG_TT
Cuts at: 1563 5673 6046 1563
Size: 4110 373 3063
Fragments arranged by size:
4110 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2068 3538 4267 4508 5609 6993 7261 1632
Size: 436 1470 729 241 1101 1384 268 1917
Fragments arranged by size:
1917 1470 1384 1101 729 436 268 241
AfeI AGC'GCT
Cuts at: 2418 2418
Size: 7546
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7349
AflIII A'CryG_T
Cuts at: 1203 4014 4407 6445 7422 1203
Size: 2811 393 2038 977 1327
Fragments arranged by size:
2811 2038 1327 977 393
AgeI A'CCGG_T
Cuts at: 4206 6377 4206
Size: 2171 5375
AhdI GACnn_n'nnGTC
Cuts at: 6275 6275
Size: 7546
AleI CACnn'nnGTG
Cuts at: 1166 3110 3402 1166
Size: 1944 292 5310
Fragments arranged by size:
5310 1944 292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2843 3038 3489 1184
Size: 1659 195 451 5241
Fragments arranged by size:
5241 1659 451 195
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4592 4624 4592
Size: 32 7514
AlwNI CAG_nnn'CTG
Cuts at: 2191 4435 5289 6861 2191
Size: 2244 854 1572 2876
Fragments arranged by size:
2876 2244 1572 854
ApaLI G'TGCA_C
Cuts at: 4148 4244 5603 6759 4148
Size: 96 1359 1156 4935
Fragments arranged by size:
4935 1359 1156 96
ApoI r'AATT_y
Cuts at: 1493 4855 5081 5233 7342 1493
Size: 3362 226 152 2109 1697
Fragments arranged by size:
3362 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6100 160
Size: 872 5068 1606
Fragments arranged by size:
5068 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7546
AvaI C'yCGr_G
Cuts at: 1870 1943 2535 2579 2674 3863 4107 4751 4871
Size: 73 592 44 95 1189 244 644 120
Cuts at: 4871 1870
Size: 4545
Fragments arranged by size:
4545 1189 644 592 244 120 95 73
44
BamHI G'GATC_C
Cuts at: 4876 5243 4876
Size: 367 7179
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2698 2947 3056 3698 4867
Size: 325 217 627 911 249 109 642 1169
Cuts at: 4867 6322 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1169 911 642 627 325 249
217 109
BanII G_rGCy'C
Cuts at: 1391 2515 2681 3672 3864 4131 4568 4721 4752
Size: 1124 166 991 192 267 437 153 31
Cuts at: 4752 4865 1391
Size: 113 4072
Fragments arranged by size:
4072 1124 991 437 267 192 166 153
113 31
BbeI G_GCGC'C
Cuts at: 2702 2702
Size: 7546
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2767 3056 3309 3544 4815 928
Size: 971 868 289 253 235 1271 3659
Fragments arranged by size:
3659 1271 971 868 289 253 235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5737 5771 1903
Size: 34 3800 34 3678
Fragments arranged by size:
3800 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5438 6648 5438
Size: 1210 6336
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7546
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6157 136
Size: 107 122 71 631 605 4485 1525
Fragments arranged by size:
4485 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7541 7541
Size: 7546
BlpI GC'TnA_GC
Cuts at: 2279 2439 2894 3026 3182 4963 2279
Size: 160 455 132 156 1781 4862
Fragments arranged by size:
4862 1781 455 160 156 132
Bme1580I G_kGCm'C
Cuts at: 1790 2952 4152 4248 4393 5607 6763 1790
Size: 1162 1200 96 145 1214 1156 2573
Fragments arranged by size:
2573 1214 1200 1162 1156 145 96
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7546
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2728 3783 4372
Size: 416 53 83 399 24 1311 1055 589
Cuts at: 4372 4499 5277 6235 442
Size: 127 778 958 1753
Fragments arranged by size:
1753 1311 1055 958 778 589 416 399
127 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4656 1056
Size: 3600 3946
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4150 4150
Size: 7546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 3161 3224 3585 3596 3828 3878 4460
Size: 839 1083 63 361 11 232 50 582
Cuts at: 4460 4552 4766 6206 1239
Size: 92 214 1440 2579
Fragments arranged by size:
2579 1440 1083 839 582 361 232 214
92 63 50 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5671 6536 6834 5671
Size: 865 298 6383
Fragments arranged by size:
6383 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2029 6209 882
Size: 617 530 4180 2219
Fragments arranged by size:
4180 2219 617 530
BsaAI yAC'GTr
Cuts at: 493 1204 2715 3715 4147 5337 493
Size: 711 1511 1000 432 1190 2702
Fragments arranged by size:
2702 1511 1190 1000 711 432
BsaBI GATnn'nnATC
Cuts at: 3140 7540 3140
Size: 4400 3146
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2588 2699 3346 5353
Size: 53 83 186 936 1055 111 647 2007
Cuts at: 5353 5735 6370 275
Size: 382 635 1451
Fragments arranged by size:
2007 1451 1055 936 647 635 382 186
111 83 53
BsaWI w'CCGG_w
Cuts at: 2408 4206 4713 5979 6377 6651 6798 7209 2408
Size: 1798 507 1266 398 274 147 411 2745
Fragments arranged by size:
2745 1798 1266 507 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3625 3655 1178
Size: 30 165 30 2222 30 5069
Fragments arranged by size:
5069 2222 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2146 2318 2386 2516 2638 2659 2662 2677
Size: 960 172 68 130 122 21 3 15
Cuts at: 2677 3143 3146 3515 3896 1186
Size: 466 3 369 381 4836
Fragments arranged by size:
4836 960 466 381 369 172 130 122
68 21 15 3 3
BseYI C'CCAG_C
Cuts at: 2191 2308 2481 2592 2658 2957 3886 3974 4078
Size: 117 173 111 66 299 929 88 104
Cuts at: 4078 4428 6749 7245 2191
Size: 350 2321 496 2492
Fragments arranged by size:
2492 2321 929 496 350 299 173 117
111 104 88 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3449 3497 4211 1163
Size: 573 1713 48 714 4498
Fragments arranged by size:
4498 1713 714 573 48
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3655 4368 4737 4817 4996 5757
Size: 275 186 1654 713 369 80 179 761
Cuts at: 5757 6785 1540
Size: 1028 2301
Fragments arranged by size:
2301 1654 1028 761 713 369 275 186
179 80
BsiHKAI G_wGCw'C
Cuts at: 2657 2741 3543 3786 3864 4152 4248 4568 4721
Size: 84 802 243 78 288 96 320 153
Cuts at: 4721 4752 5607 5692 6763 2657
Size: 31 855 85 1071 3440
Fragments arranged by size:
3440 1071 855 802 320 288 243 153
96 85 84 78 31
BsmI GAATG_Cn'
Cuts at: 5057 5150 5057
Size: 93 7453
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2515 2657 2681 2741 2952 3543 3672
Size: 399 725 142 24 60 211 591 129
Cuts at: 3672 3786 3864 4131 4152 4248 4393 4568 4721
Size: 114 78 267 21 96 145 175 153
Cuts at: 4721 4752 4865 5607 5692 6763 1391
Size: 31 113 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 725 591 399 267 211
175 153 145 142 129 114 113 96
85 78 60 31 24 21
BspEI T'CCGG_A
Cuts at: 7209 7209
Size: 7546
BspHI T'CATG_A
Cuts at: 5435 7165 5435
Size: 1730 5816
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2148 3072 4245 4901 844
Size: 1304 924 1173 656 3489
Fragments arranged by size:
3489 1304 1173 924 656
BsrBI CCG'CTC
Cuts at: 1394 1823 2367 3868 4819 5433 1394
Size: 429 544 1501 951 614 3507
Fragments arranged by size:
3507 1501 951 614 544 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3696 6041 6215 65
Size: 1183 2448 2345 174 1396
Fragments arranged by size:
2448 2345 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2615 2708 2934 3650 3873 4206 4253
Size: 110 805 93 226 716 223 333 47
Cuts at: 4253 4269 4372 4812 6190 6377 1700
Size: 16 103 440 1378 187 2869
Fragments arranged by size:
2869 1378 805 716 440 333 226 223
187 110 103 93 47 16
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7546
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7546
BssSI C'ACGA_G
Cuts at: 2789 3596 5606 6618 2789
Size: 807 2010 1012 3717
Fragments arranged by size:
3717 2010 1012 807
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4033 794
Size: 3239 4307
BstBI TT'CG_AA
Cuts at: 6350 6350
Size: 7546
BstEII G'GTnAC_C
Cuts at: 1184 2641 1184
Size: 1457 6089
BstXI CCAn_nnnn'nTGG
Cuts at: 2489 2965 2489
Size: 476 7070
BstYI r'GATC_y
Cuts at: 1972 3504 4538 4876 5243 5628 5645 7097 7183
Size: 1532 1034 338 367 385 17 1452 86
Cuts at: 7183 7195 7541 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1532 1452 1034 385 367 346 338
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2609 2609
Size: 7546
BtgI C'CryG_G
Cuts at: 513 1065 2488 2694 4362 513
Size: 552 1423 206 1668 3697
Fragments arranged by size:
3697 1668 1423 552 206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4794 487
Size: 683 56 3568 3239
Fragments arranged by size:
3568 3239 683 56
BtsI GCAGTG_nn'
Cuts at: 2827 2945 3416 4721 5062 5856 5876 2827
Size: 118 471 1305 341 794 20 4497
Fragments arranged by size:
4497 1305 794 471 341 118 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7546
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7511
DraI TTT'AAA
Cuts at: 4584 4850 4584
Size: 266 7280
DraIII CAC_nnn'GTG
Cuts at: 4507 4507
Size: 7546
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6553 809
Size: 507 5237 1802
Fragments arranged by size:
5237 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2559 2617 3194
Size: 54 1425 50 275 18 729 58 577
Cuts at: 3194 3652 4315 4814 4993 5882 7511 8
Size: 458 663 499 179 889 1629 43
Fragments arranged by size:
1629 1425 889 729 663 577 499 458
275 179 58 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3652 4814 4993 1537
Size: 275 1840 1162 179 4090
Fragments arranged by size:
4090 1840 1162 275 179
EarI CTCTTCn'nnn_
Cuts at: 1353 2330 4475 5476 1353
Size: 977 2145 1001 3423
Fragments arranged by size:
3423 2145 1001 977
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2873 4730 6133 6507 6653 387
Size: 1064 138 1284 1857 1403 374 146 1280
Fragments arranged by size:
1857 1403 1284 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3862 4566 4719 4750 3862
Size: 704 153 31 6658
Fragments arranged by size:
6658 704 153 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2078 3161 3224 3538 3585 3596
Size: 393 436 10 1083 63 314 47 11
Cuts at: 3596 3828 3878 4267 4460 4508 4552 4766 5609
Size: 232 50 389 193 48 44 214 843
Cuts at: 5609 6206 6993 7261 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1083 843 787 597 436 393 389
314 268 232 214 193 63 50 48
47 44 11 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2116 2831 3833 2116
Size: 715 1002 5829
Fragments arranged by size:
5829 1002 715
EcoO109I rG'GnC_Cy
Cuts at: 2361 2532 2719 3331 4323 4572 2361
Size: 171 187 612 992 249 5335
Fragments arranged by size:
5335 992 612 249 187 171
EcoRI G'AATT_C
Cuts at: 4855 5233 4855
Size: 378 7168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3241 3241
Size: 7546
FspI TGC'GCA
Cuts at: 6052 6052
Size: 7546
HaeII r_GCGC'y
Cuts at: 1678 2420 2702 4254 4424 6693 1678
Size: 742 282 1552 170 2269 2531
Fragments arranged by size:
2531 2269 1552 742 282 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 3489 3521 3579
Size: 32 135 32 794 32 1091 32 58
Cuts at: 3579 3611 3625 3657 3953 3985 4703 4735 4827
Size: 32 14 32 296 32 718 32 92
Cuts at: 4827 4859 6187 6219 6261 6293 1373
Size: 32 1328 32 42 32 2626
Fragments arranged by size:
2626 1328 1091 794 718 296 135 92
58 42 32 32 32 32 32 32
32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3967 4519 4890 5136 669
Size: 988 281 2029 552 371 246 3079
Fragments arranged by size:
3079 2029 988 552 371 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7546
HpaI GTT'AAC
Cuts at: 5136 5136
Size: 7546
KasI G'GCGC_C
Cuts at: 2698 2698
Size: 7546
KpnI G_GTAC'C
Cuts at: 4871 4871
Size: 7546
MfeI C'AATT_G
Cuts at: 5145 5145
Size: 7546
MluI A'CGCG_T
Cuts at: 7422 7422
Size: 7546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3656 3668 3984 6660 6844 1305
Size: 2351 12 316 2676 184 2007
Fragments arranged by size:
2676 2351 2007 316 184 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3196 10
Size: 54 1425 343 1364 4360
Fragments arranged by size:
4360 1425 1364 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2804 3110 3402 3663 3779 5504 5863
Size: 648 1638 306 292 261 116 1725 359
Cuts at: 5863 6022 518
Size: 159 2042
Fragments arranged by size:
2042 1725 1638 648 359 306 292 261
159 116
MspA1I CmG'CkG
Cuts at: 1606 2662 2705 3030 3448 3511 3574 4140 4364
Size: 1056 43 325 418 63 63 566 224
Cuts at: 4364 4480 4593 4962 5639 6787 7032 1606
Size: 116 113 369 677 1148 245 2120
Fragments arranged by size:
2120 1148 1056 677 566 418 369 325
245 224 116 113 63 63 43
NaeI GCC'GGC
Cuts at: 2710 4255 4374 2710
Size: 1545 119 5882
Fragments arranged by size:
5882 1545 119
NarI GG'CG_CC
Cuts at: 2699 2699
Size: 7546
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6994
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7546
NgoMIV G'CCGG_C
Cuts at: 2708 4253 4372 2708
Size: 1545 119 5882
Fragments arranged by size:
5882 1545 119
NheI G'CTAG_C
Cuts at: 1052 4652 1052
Size: 3600 3946
NotI GC'GGCC_GC
Cuts at: 4993 4993
Size: 7546
NspI r_CATG'y
Cuts at: 1132 1229 2471 3586 4904 6449 1132
Size: 97 1242 1115 1318 1545 2229
Fragments arranged by size:
2229 1545 1318 1242 1115 97
PasI CC'CwG_GG
Cuts at: 1348 2458 1348
Size: 1110 6436
PciI A'CATG_T
Cuts at: 6445 6445
Size: 7546
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3424 3691 3856 1108
Size: 2316 267 165 4798
Fragments arranged by size:
4798 2316 267 165
PfoI T'CCnGG_A
Cuts at: 3086 3317 3086
Size: 231 7315
PmeI GTTT'AAAC
Cuts at: 4850 4850
Size: 7546
PmlI CAC'GTG
Cuts at: 2715 3715 4147 2715
Size: 1000 432 6114
Fragments arranged by size:
6114 1000 432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3654 3686 4751 4783 5615 5647 7128 7160 3654
Size: 32 1065 32 832 32 1481 32 4040
Fragments arranged by size:
4040 1481 1065 832 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2361 2719 3331 4323 2361
Size: 358 612 992 5584
Fragments arranged by size:
5584 992 612 358
PshAI GACnn'nnGTC
Cuts at: 1613 4736 1613
Size: 3123 4423
PsiI TTA'TAA
Cuts at: 3750 5116 3750
Size: 1366 6180
PspXI vC'TCGA_Gb
Cuts at: 3863 4751 3863
Size: 888 6658
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4178 4210 4178
Size: 32 7514
PstI C_TGCA'G
Cuts at: 830 2727 3069 4455 4898 830
Size: 1897 342 1386 443 3478
Fragments arranged by size:
3478 1897 1386 443 342
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7546
PvuII CAG'CTG
Cuts at: 2662 3448 3511 3574 4593 2662
Size: 786 63 63 1019 5615
Fragments arranged by size:
5615 1019 786 63 63
SacI G_AGCT'C
Cuts at: 3864 4568 4721 4752 3864
Size: 704 153 31 6658
Fragments arranged by size:
6658 704 153 31
SacII CC_GC'GG
Cuts at: 4365 4365
Size: 7546
SalI G'TCGA_C
Cuts at: 1936 4517 4888 1936
Size: 2581 371 4594
Fragments arranged by size:
4594 2581 371
SbfI CC_TGCA'GG
Cuts at: 2727 4898 2727
Size: 2171 5375
ScaI AGT'ACT
Cuts at: 5794 7506 5794
Size: 1712 5834
SfcI C'TryA_G
Cuts at: 826 1045 2602 2723 3065 4451 4894 6029 6710
Size: 219 1557 121 342 1386 443 1135 681
Cuts at: 6710 6901 826
Size: 191 1471
Fragments arranged by size:
1557 1471 1386 1135 681 443 342 219
191 121
SfoI GGC'GCC
Cuts at: 2700 2700
Size: 7546
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7546
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7546
SmaI CCC'GGG
Cuts at: 1872 2537 2581 4109 4873 1872
Size: 665 44 1528 764 4545
Fragments arranged by size:
4545 1528 764 665 44
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3863 4751 5650 6551 6813 820
Size: 197 926 1920 888 899 901 262 1553
Fragments arranged by size:
1920 1553 926 901 899 888 262 197
SnaBI TAC'GTA
Cuts at: 493 5337 493
Size: 4844 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7546
SphI G_CATG'C
Cuts at: 1132 2471 3586 4904 1132
Size: 1339 1115 1318 3774
Fragments arranged by size:
3774 1339 1318 1115
SspI AAT'ATT
Cuts at: 5 52 3758 5470 5
Size: 47 3706 1712 2081
Fragments arranged by size:
3706 2081 1712 47
StuI AGG'CCT
Cuts at: 1517 2255 2729 2753 1517
Size: 738 474 24 6310
Fragments arranged by size:
6310 738 474 24
StyI C'CwwG_G
Cuts at: 513 1065 2178 2229 2256 2400 2748 2832 3534
Size: 552 1113 51 27 144 348 84 702
Cuts at: 3534 4341 4985 513
Size: 807 644 3074
Fragments arranged by size:
3074 1113 807 702 644 552 348 144
84 51 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2629 2782 2851 4518 5573 5590 5743
Size: 1255 1049 153 69 1667 1055 17 153
Cuts at: 5743 5928 6373 325
Size: 185 445 1498
Fragments arranged by size:
1667 1498 1255 1055 1049 445 185 153
153 69 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3420 4194 4380
Size: 274 80 33 51 1443 1443 774 186
Cuts at: 4380 5792 7504 96
Size: 1412 1712 138
Fragments arranged by size:
1712 1443 1443 1412 774 274 186 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2015 2047 3040 3072 4322 4354 7506 7538 2015
Size: 32 993 32 1250 32 3152 32 2023
Fragments arranged by size:
3152 2023 1250 993 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7546
XbaI T'CTAG_A
Cuts at: 4882 4882
Size: 7546
XhoI C'TCGA_G
Cuts at: 1943 3863 4751 1943
Size: 1920 888 4738
Fragments arranged by size:
4738 1920 888
XmaI C'CCGG_G
Cuts at: 1870 2535 2579 4107 4871 1870
Size: 665 44 1528 764 4545
Fragments arranged by size:
4545 1528 764 665 44
XmnI GAAnn'nnTTC
Cuts at: 4208 5675 4208
Size: 1467 6079
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5354 276
Size: 53 83 186 936 3820 2468
Fragments arranged by size:
3820 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI
PacI PspOMI RsrII SanDI SapI SexAI SfiI SrfI
SwaI XcmI