Restriction map for
pFN21AA0564
(Circular) (Six-base) MAPSORT of: pFN21AA0564.seq Check: 1090 from: 1 to: 10420
pFN21AA0564 10420 bp
With 182 enzymes: SgfI *
September 16, 2010 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2077 4401 2077
Size: 2324 8096
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8230 278
Size: 53 83 186 936 6694 2468
Fragments arranged by size:
6694 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6434 6449 7763 1937
Size: 4497 15 1314 4594
Fragments arranged by size:
4594 4497 1314 15
Acc65I G'GTAC_C
Cuts at: 4754 7741 4754
Size: 2987 7433
AclI AA'CG_TT
Cuts at: 1563 5141 8547 8920 1563
Size: 3578 3406 373 3063
Fragments arranged by size:
3578 3406 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3715 4181 4442 4844 5001 5319 7391 7601
Size: 2083 466 261 402 157 318 2072 210
Cuts at: 7601 8483 9867 10135 1632
Size: 882 1384 268 1917
Fragments arranged by size:
2083 2072 1917 1384 882 466 402 318
268 261 210 157
AfeI AGC'GCT
Cuts at: 3086 3086
Size: 10420
AflII C'TTAA_G
Cuts at: 820 1017 2828 820
Size: 197 1811 8412
Fragments arranged by size:
8412 1811 197
AflIII A'CryG_T
Cuts at: 1203 4574 7416 9319 10296 1203
Size: 3371 2842 1903 977 1327
Fragments arranged by size:
3371 2842 1903 1327 977
AgeI A'CCGG_T
Cuts at: 9251 9251
Size: 10420
AhdI GACnn_n'nnGTC
Cuts at: 4983 5163 9149 4983
Size: 180 3986 6254
Fragments arranged by size:
6254 3986 180
AleI CACnn'nnGTG
Cuts at: 1166 3781 1166
Size: 2615 7805
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2090 1184
Size: 906 9514
AlwNI CAG_nnn'CTG
Cuts at: 3787 6941 8163 9735 3787
Size: 3154 1222 1572 4472
Fragments arranged by size:
4472 3154 1572 1222
ApaI G_GGCC'C
Cuts at: 5608 5608
Size: 10420
ApaLI G'TGCA_C
Cuts at: 4215 6956 8477 9633 4215
Size: 2741 1521 1156 5002
Fragments arranged by size:
5002 2741 1521 1156
ApoI r'AATT_y
Cuts at: 1493 2208 2707 3797 3996 4642 4970 5702 6006
Size: 715 499 1090 199 646 328 732 304
Cuts at: 6006 6078 7365 7729 7955 8107 10216 1493
Size: 72 1287 364 226 152 2109 1697
Fragments arranged by size:
2109 1697 1287 1090 732 715 646 499
364 328 304 226 199 152 72
AseI AT'TA_AT
Cuts at: 160 1032 5337 8974 160
Size: 872 4305 3637 1606
Fragments arranged by size:
4305 3637 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10420
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 2152 4407 4814 7745 1870
Size: 73 84 125 2255 407 2931 4545
Fragments arranged by size:
4545 2931 2255 407 125 84 73
AvrII C'CTAG_G
Cuts at: 4735 4735
Size: 10420
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4425 4458 5240 5273 4425
Size: 33 782 33 9572
Fragments arranged by size:
9572 782 33 33
BamHI G'GATC_C
Cuts at: 3943 7750 8117 3943
Size: 3807 367 6246
Fragments arranged by size:
6246 3807 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2032 3217 4754 5499 5518
Size: 325 217 627 245 1185 1537 745 19
Cuts at: 5518 7741 9196 618
Size: 2223 1455 1842
Fragments arranged by size:
2223 1842 1537 1455 1185 745 627 325
245 217 19
BanII G_rGCy'C
Cuts at: 1391 4815 4854 5608 7739 1391
Size: 3424 39 754 2131 4072
Fragments arranged by size:
4072 3424 2131 754 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3651 4500 4633 7379 928
Size: 971 1752 849 133 2746 3969
Fragments arranged by size:
3969 2746 1752 971 849 133
BbvCI CC'TCA_GC
Cuts at: 4889 4889
Size: 10420
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2387 2421 8611 8645 1903
Size: 34 50 34 366 34 6190 34 3678
Fragments arranged by size:
6190 3678 366 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2203 3392 4029 4426 5070 5268 7651 8312 9522
Size: 1189 637 397 644 198 2383 661 1210
Cuts at: 9522 2203
Size: 3101
Fragments arranged by size:
3101 2383 1210 1189 661 644 637 397
198
BclI T'GATC_A
Cuts at: 1548 2482 6016 7600 1548
Size: 934 3534 1584 4368
Fragments arranged by size:
4368 3534 1584 934
BfrBI ATG'CAT
Cuts at: 7381 7381
Size: 10420
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 6750 9031
Size: 107 122 71 631 605 377 4701 2281
Cuts at: 9031 136
Size: 1525
Fragments arranged by size:
4701 2281 1525 631 605 377 122 107
71
BglII A'GATC_T
Cuts at: 6127 7689 10415 6127
Size: 1562 2726 6132
Fragments arranged by size:
6132 2726 1562
BlpI GC'TnA_GC
Cuts at: 2631 3611 7837 2631
Size: 980 4226 5214
Fragments arranged by size:
5214 4226 980
Bme1580I G_kGCm'C
Cuts at: 1790 2035 3572 4219 5608 6960 8481 9637 1790
Size: 245 1537 647 1389 1352 1521 1156 2573
Fragments arranged by size:
2573 1537 1521 1389 1352 1156 647 245
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10420
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4071 5445 6135
Size: 416 53 83 399 24 2654 1374 690
Cuts at: 6135 8151 9109 442
Size: 2016 958 1753
Fragments arranged by size:
2654 2016 1753 1374 958 690 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4975 7053 7092 7127 7317 9080 1239
Size: 3736 2078 39 35 190 1763 2579
Fragments arranged by size:
3736 2579 2078 1763 190 39 35
Bpu10I CC'TnA_GC
Cuts at: 4848 4889 4848
Size: 41 10379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5795 7229 7510 7545 8545 9410 9708 5795
Size: 1434 281 35 1000 865 298 6507
Fragments arranged by size:
6507 1434 1000 865 298 281 35
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4991 6157 6805 7548 9083 882
Size: 617 3492 1166 648 743 1535 2219
Fragments arranged by size:
3492 2219 1535 1166 743 648 617
BsaAI yAC'GTr
Cuts at: 493 1204 4575 6694 8211 493
Size: 711 3371 2119 1517 2702
Fragments arranged by size:
3371 2702 2119 1517 711
BsaBI GATnn'nnATC
Cuts at: 2999 5081 6270 6418 6620 7500 10414 2999
Size: 2082 1189 148 202 880 2914 3005
Fragments arranged by size:
3005 2914 2082 1189 880 202 148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8227 8609 9244 275
Size: 53 83 186 936 6694 382 635 1451
Fragments arranged by size:
6694 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3186 8853 9251 9525 9672 10083 3186
Size: 5667 398 274 147 411 3523
Fragments arranged by size:
5667 3523 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2841 2871 7214 7244 1178
Size: 30 165 30 1438 30 4343 30 4354
Fragments arranged by size:
4354 4343 1438 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 3683 4906 5960 6330 7277 1186
Size: 829 1668 1223 1054 370 947 4329
Fragments arranged by size:
4329 1668 1223 1054 947 829 370
BseYI C'CCAG_C
Cuts at: 3787 5524 5954 9623 10119 3787
Size: 1737 430 3669 496 4088
Fragments arranged by size:
4088 3669 1737 496 430
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2114 2481 2511 2520 5126 5877 1163
Size: 573 378 367 30 9 2606 751 5706
Fragments arranged by size:
5706 2606 751 573 378 367 30 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2126 7870 8631 9659 1540
Size: 275 186 125 5744 761 1028 2301
Fragments arranged by size:
5744 2301 1028 761 275 186 125
BsiHKAI G_wGCw'C
Cuts at: 4219 4815 6960 8481 8566 9637 4219
Size: 596 2145 1521 85 1071 5002
Fragments arranged by size:
5002 2145 1521 1071 596 85
BsmI GAATG_Cn'
Cuts at: 3517 3549 3568 6869 7931 8024 3517
Size: 32 19 3301 1062 93 5913
Fragments arranged by size:
5913 3301 1062 93 32 19
BsmBI CGTCTCn'nnnn_
Cuts at: 5621 5621
Size: 10420
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 3572 4219 4815 4854 5608 6960
Size: 399 245 1537 647 596 39 754 1352
Cuts at: 6960 7739 8481 8566 9637 1391
Size: 779 742 85 1071 2174
Fragments arranged by size:
2174 1537 1352 1071 779 754 742 647
596 399 245 85 39
BspEI T'CCGG_A
Cuts at: 3186 10083 3186
Size: 6897 3523
BspHI T'CATG_A
Cuts at: 3049 8309 10039 3049
Size: 5260 1730 3430
Fragments arranged by size:
5260 3430 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2077 4401 7775 844
Size: 1233 2324 3374 3489
Fragments arranged by size:
3489 3374 2324 1233
BsrBI CCG'CTC
Cuts at: 1394 1823 8307 1394
Size: 429 6484 3507
Fragments arranged by size:
6484 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2403 3738 4747 5352 8915 9089 65
Size: 1183 1155 1335 1009 605 3563 174 1396
Fragments arranged by size:
3563 1396 1335 1183 1155 1009 605 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2147 6768 7203 9064 9251 1700
Size: 110 337 4621 435 1861 187 2869
Fragments arranged by size:
4621 2869 1861 435 337 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10420
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10420
BssSI C'ACGA_G
Cuts at: 8480 9492 8480
Size: 1012 9408
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2092 2498 4646 794
Size: 1298 406 2148 6568
Fragments arranged by size:
6568 2148 1298 406
BstBI TT'CG_AA
Cuts at: 5002 9224 5002
Size: 4222 6198
BstEII G'GTnAC_C
Cuts at: 1184 3205 1184
Size: 2021 8399
BstYI r'GATC_y
Cuts at: 1972 2302 2454 3704 3943 5282 6127 6891 7689
Size: 330 152 1250 239 1339 845 764 798
Cuts at: 7689 7750 8117 8502 8519 9971 10057 10069 10415
Size: 61 367 385 17 1452 86 12 346
Cuts at: 10415 1972
Size: 1977
Fragments arranged by size:
1977 1452 1339 1250 845 798 764 385
367 346 330 239 152 86 61 17
12
BstZ17I GTA'TAC
Cuts at: 6435 6435
Size: 10420
Bsu36I CC'TnA_GG
Cuts at: 3394 3888 4759 3394
Size: 494 871 9055
Fragments arranged by size:
9055 871 494
BtgI C'CryG_G
Cuts at: 513 1065 2044 7653 513
Size: 552 979 5609 3280
Fragments arranged by size:
5609 3280 979 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9681
Fragments arranged by size:
9681 683 56
BtsI GCAGTG_nn'
Cuts at: 3483 6470 7936 8730 8750 3483
Size: 2987 1466 794 20 5153
Fragments arranged by size:
5153 2987 1466 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10420
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10385
DraI TTT'AAA
Cuts at: 3081 3663 4584 7724 3081
Size: 582 921 3140 5777
Fragments arranged by size:
5777 3140 921 582
DraIII CAC_nnn'GTG
Cuts at: 3559 6143 3559
Size: 2584 7836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6552 9427 809
Size: 507 5236 2875 1802
Fragments arranged by size:
5236 2875 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2123 5599 7201
Size: 54 1425 50 275 18 293 3476 1602
Cuts at: 7201 7867 8756 10385 8
Size: 666 889 1629 43
Fragments arranged by size:
3476 1629 1602 1425 889 666 293 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2123 7867 1537
Size: 275 311 5744 4090
Fragments arranged by size:
5744 4090 311 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2352 2778 3695 4657 6155 6724 6825 8350
Size: 999 426 917 962 1498 569 101 1525
Cuts at: 8350 1353
Size: 3423
Fragments arranged by size:
3423 1525 1498 999 962 917 569 426
101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6732 9007 9381 9527 387
Size: 1064 138 5143 2275 374 146 1280
Fragments arranged by size:
5143 2275 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4813 4813
Size: 10420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3715 4181 4442 4844 4975 5001 5319
Size: 393 2083 466 261 402 131 26 318
Cuts at: 5319 7053 7092 7127 7317 7391 7601 8483 9080
Size: 1734 39 35 190 74 210 882 597
Cuts at: 9080 9867 10135 1239
Size: 787 268 1524
Fragments arranged by size:
2083 1734 1524 882 787 597 466 402
393 318 268 261 210 190 131 74
39 35 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 4734 5538 6925 2084
Size: 2650 804 1387 5579
Fragments arranged by size:
5579 2650 1387 804
EcoO109I rG'GnC_Cy
Cuts at: 2333 3874 5605 6673 6761 2333
Size: 1541 1731 1068 88 5992
Fragments arranged by size:
5992 1731 1541 1068 88
EcoRI G'AATT_C
Cuts at: 3797 6078 7729 8107 3797
Size: 2281 1651 378 6110
Fragments arranged by size:
6110 2281 1651 378
EcoRV GAT'ATC
Cuts at: 5470 6319 7669 5470
Size: 849 1350 8221
Fragments arranged by size:
8221 1350 849
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3264 4076 4496 3264
Size: 812 420 9188
Fragments arranged by size:
9188 812 420
FspI TGC'GCA
Cuts at: 8926 8926
Size: 10420
HaeII r_GCGC'y
Cuts at: 1678 3088 9567 1678
Size: 1410 6479 2531
Fragments arranged by size:
6479 2531 1410
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2439 2471 3298 3330 4929
Size: 32 135 32 867 32 827 32 1599
Cuts at: 4929 4961 4969 5001 5149 5181 6008 6040 6052
Size: 32 8 32 148 32 827 32 12
Cuts at: 6052 6084 6119 6151 6365 6397 6510 6542 6635
Size: 32 35 32 214 32 113 32 93
Cuts at: 6635 6667 9061 9093 9135 9167 1373
Size: 32 2394 32 42 32 2626
Fragments arranged by size:
2626 2394 1599 867 827 827 214 148
135 113 93 42 35 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 12 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3245 4378 4948 5124 6450 7764
Size: 988 281 1307 1133 570 176 1326 1314
Cuts at: 7764 8010 669
Size: 246 3079
Fragments arranged by size:
3079 1326 1314 1307 1133 988 570 281
246 176
HindIII A'AGCT_T
Cuts at: 748 3171 4865 748
Size: 2423 1694 6303
Fragments arranged by size:
6303 2423 1694
HpaI GTT'AAC
Cuts at: 8010 8010
Size: 10420
KpnI G_GTAC'C
Cuts at: 4758 7745 4758
Size: 2987 7433
MfeI C'AATT_G
Cuts at: 2844 5160 6247 8019 2844
Size: 2316 1087 1772 5245
Fragments arranged by size:
5245 2316 1772 1087
MluI A'CGCG_T
Cuts at: 10296 10296
Size: 10420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2655 3971 4586 5000 7267 9534 9718 1305
Size: 1350 1316 615 414 2267 2267 184 2007
Fragments arranged by size:
2267 2267 2007 1350 1316 615 414 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 8598
Fragments arranged by size:
8598 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3215 3781 5370 8378 8737 8896 518
Size: 648 2049 566 1589 3008 359 159 2042
Fragments arranged by size:
3008 2049 2042 1589 648 566 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2963 5110 5524 6047 7634 7836 8513
Size: 516 841 2147 414 523 1587 202 677
Cuts at: 8513 9661 9906 1606
Size: 1148 245 2120
Fragments arranged by size:
2147 2120 1587 1148 841 677 523 516
414 245 202
NaeI GCC'GGC
Cuts at: 2149 7205 2149
Size: 5056 5364
NcoI C'CATG_G
Cuts at: 513 1065 7653 513
Size: 552 6588 3280
Fragments arranged by size:
6588 3280 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10420
NgoMIV G'CCGG_C
Cuts at: 2147 7203 2147
Size: 5056 5364
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10420
NotI GC'GGCC_GC
Cuts at: 7867 7867
Size: 10420
NsiI A_TGCA'T
Cuts at: 7383 7383
Size: 10420
NspI r_CATG'y
Cuts at: 1132 1229 2071 3011 4286 5403 6688 7180 7385
Size: 97 842 940 1275 1117 1285 492 205
Cuts at: 7385 7439 7778 9323 1132
Size: 54 339 1545 2229
Fragments arranged by size:
2229 1545 1285 1275 1117 940 842 492
339 205 97 54
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10420
PciI A'CATG_T
Cuts at: 9319 9319
Size: 10420
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3787 7118 1108
Size: 2679 3331 4410
Fragments arranged by size:
4410 3331 2679
PfoI T'CCnGG_A
Cuts at: 6975 6975
Size: 10420
PmeI GTTT'AAAC
Cuts at: 7724 7724
Size: 10420
PmlI CAC'GTG
Cuts at: 4575 6694 4575
Size: 2119 8301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7253 7285 8489 8521 10002 10034 7253
Size: 32 1204 32 1481 32 7639
Fragments arranged by size:
7639 1481 1204 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2333 3874 6673 2333
Size: 1541 2799 6080
Fragments arranged by size:
6080 2799 1541
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10420
PsiI TTA'TAA
Cuts at: 5243 5688 7990 5243
Size: 445 2302 7673
Fragments arranged by size:
7673 2302 445
PspOMI G'GGCC_C
Cuts at: 5604 5604
Size: 10420
PstI C_TGCA'G
Cuts at: 830 6561 7772 830
Size: 5731 1211 3478
Fragments arranged by size:
5731 3478 1211
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10420
PvuII CAG'CTG
Cuts at: 2963 5110 6047 7634 2963
Size: 2147 937 1587 5749
Fragments arranged by size:
5749 2147 1587 937
SacI G_AGCT'C
Cuts at: 4815 4815
Size: 10420
SalI G'TCGA_C
Cuts at: 1936 6448 7762 1936
Size: 4512 1314 4594
Fragments arranged by size:
4594 4512 1314
SbfI CC_TGCA'GG
Cuts at: 7772 7772
Size: 10420
ScaI AGT'ACT
Cuts at: 2372 7475 8668 10380 2372
Size: 5103 1193 1712 2412
Fragments arranged by size:
5103 2412 1712 1193
SexAI A'CCwGG_T
Cuts at: 6795 6795
Size: 10420
SfcI C'TryA_G
Cuts at: 826 1045 2268 3293 3452 3695 6208 6557 7768
Size: 219 1223 1025 159 243 2513 349 1211
Cuts at: 7768 8903 9584 9775 826
Size: 1135 681 191 1471
Fragments arranged by size:
2513 1471 1223 1211 1135 1025 681 349
243 219 191 159
SfiI GGCCn_nnn'nGGCC
Cuts at: 2049 2049
Size: 10420
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10420
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10420
SmaI CCC'GGG
Cuts at: 1872 7747 1872
Size: 5875 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2828 4814 5810 7208 7489 7560
Size: 197 926 885 1986 996 1398 281 71
Cuts at: 7560 8524 9425 9687 820
Size: 964 901 262 1553
Fragments arranged by size:
1986 1553 1398 996 964 926 901 885
281 262 197 71
SnaBI TAC'GTA
Cuts at: 493 8211 493
Size: 7718 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10420
SphI G_CATG'C
Cuts at: 1132 2071 7385 7778 1132
Size: 939 5314 393 3774
Fragments arranged by size:
5314 3774 939 393
SspI AAT'ATT
Cuts at: 5 52 2182 8344 5
Size: 47 2130 6162 2081
Fragments arranged by size:
6162 2130 2081 47
StuI AGG'CCT
Cuts at: 1517 6744 1517
Size: 5227 5193
StyI C'CwwG_G
Cuts at: 513 1065 2724 2741 3744 4321 4735 4906 7341
Size: 552 1659 17 1003 577 414 171 2435
Cuts at: 7341 7653 7662 7859 513
Size: 312 9 197 3074
Fragments arranged by size:
3074 2435 1659 1003 577 552 414 312
197 171 17 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6154 6737 7629 8447 8464 8617 8802
Size: 1255 4574 583 892 818 17 153 185
Cuts at: 8802 9247 325
Size: 445 1498
Fragments arranged by size:
4574 1498 1255 892 818 583 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2370 4441 5418
Size: 274 80 33 51 1443 393 2071 977
Cuts at: 5418 7473 8666 10378 96
Size: 2055 1193 1712 138
Fragments arranged by size:
2071 2055 1712 1443 1193 977 393 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3351 3383 4827 4859 7214 7246 10380 10412 3351
Size: 32 1444 32 2355 32 3134 32 3359
Fragments arranged by size:
3359 3134 2355 1444 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10420
XbaI T'CTAG_A
Cuts at: 5770 7756 5770
Size: 1986 8434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2671 5107 2671
Size: 2436 7984
XhoI C'TCGA_G
Cuts at: 1943 4814 1943
Size: 2871 7549
XmaI C'CCGG_G
Cuts at: 1870 7745 1870
Size: 5875 4545
XmnI GAAnn'nnTTC
Cuts at: 4032 4416 4611 5654 8549 4032
Size: 384 195 1043 2895 5903
Fragments arranged by size:
5903 2895 1043 384 195
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8228 276
Size: 53 83 186 936 6694 2468
Fragments arranged by size:
6694 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BbeI BplI BsiWI BstXI FseI FspAI
KasI NarI NruI PacI PspXI PsrI RsrII SacII
SanDI SapI SfoI SrfI SwaI