Restriction map for
pFN21AA0563
(Circular) (Six-base) MAPSORT of: pFN21AA0563.seq Check: 6779 from: 1 to: 9607
pFN21AA0563 9607 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7417 278
Size: 53 83 186 936 5881 2468
Fragments arranged by size:
5881 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5697 6007 6950 1937
Size: 3760 310 943 4594
Fragments arranged by size:
4594 3760 943 310
Acc65I G'GTAC_C
Cuts at: 6928 6928
Size: 9607
AclI AA'CG_TT
Cuts at: 1563 7734 8107 1563
Size: 6171 373 3063
Fragments arranged by size:
6171 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2474 2916 4095 4389 5199 6123 7670 9054
Size: 842 442 1179 294 810 924 1547 1384
Cuts at: 9054 9322 1632
Size: 268 1917
Fragments arranged by size:
1917 1547 1384 1179 924 842 810 442
294 268
AflII C'TTAA_G
Cuts at: 820 1017 5018 6088 820
Size: 197 4001 1070 4339
Fragments arranged by size:
4339 4001 1070 197
AflIII A'CryG_T
Cuts at: 1203 8506 9483 1203
Size: 7303 977 1327
Fragments arranged by size:
7303 1327 977
AgeI A'CCGG_T
Cuts at: 8438 8438
Size: 9607
AhdI GACnn_n'nnGTC
Cuts at: 2704 3153 8336 2704
Size: 449 5183 3975
Fragments arranged by size:
5183 3975 449
AleI CACnn'nnGTG
Cuts at: 1166 2032 1166
Size: 866 8741
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3513 1184
Size: 2329 7278
AlwNI CAG_nnn'CTG
Cuts at: 2122 4292 4351 5494 5741 6445 7350 8922 2122
Size: 2170 59 1143 247 704 905 1572 2807
Fragments arranged by size:
2807 2170 1572 1143 905 704 247 59
ApaI G_GGCC'C
Cuts at: 2066 2271 2631 3380 5516 6529 2066
Size: 205 360 749 2136 1013 5144
Fragments arranged by size:
5144 2136 1013 749 360 205
ApaLI G'TGCA_C
Cuts at: 7664 8820 7664
Size: 1156 8451
ApoI r'AATT_y
Cuts at: 1493 2317 4615 6058 6426 6916 7142 7294 9403
Size: 824 2298 1443 368 490 226 152 2109
Cuts at: 9403 1493
Size: 1697
Fragments arranged by size:
2298 2109 1697 1443 824 490 368 226
152
AscI GG'CGCG_CC
Cuts at: 5766 5766
Size: 9607
AseI AT'TA_AT
Cuts at: 160 1032 3462 6660 8161 160
Size: 872 2430 3198 1501 1606
Fragments arranged by size:
3198 2430 1606 1501 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9607
AvaI C'yCGr_G
Cuts at: 1870 1943 3981 6371 6932 1870
Size: 73 2038 2390 561 4545
Fragments arranged by size:
4545 2390 2038 561 73
BamHI G'GATC_C
Cuts at: 6937 7304 6937
Size: 367 9240
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4598 5579 6928 8383 618
Size: 325 217 627 2811 981 1349 1455 1842
Fragments arranged by size:
2811 1842 1455 1349 981 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2066 2181 2271 2631 2670 3380 4503 4584
Size: 675 115 90 360 39 710 1123 81
Cuts at: 4584 5280 5516 6378 6529 6926 1391
Size: 696 236 862 151 397 4072
Fragments arranged by size:
4072 1123 862 710 696 675 397 360
236 151 115 90 81 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2178 2703 3102 3528 4322 5888 6650
Size: 971 279 525 399 426 794 1566 762
Cuts at: 6650 928
Size: 3885
Fragments arranged by size:
3885 1566 971 794 762 525 426 399
279
BbvCI CC'TCA_GC
Cuts at: 3522 3522
Size: 9607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7798 7832 1903
Size: 34 5861 34 3678
Fragments arranged by size:
5861 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2586 6622 7499 8709 2586
Size: 4036 877 1210 3484
Fragments arranged by size:
4036 3484 1210 877
BclI T'GATC_A
Cuts at: 1548 2257 6415 1548
Size: 709 4158 4740
Fragments arranged by size:
4740 4158 709
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2626 8218 136
Size: 107 122 71 631 605 954 5592 1525
Fragments arranged by size:
5592 1525 954 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6813 9602 6813
Size: 2789 6818
BlpI GC'TnA_GC
Cuts at: 2115 2469 3504 3798 4128 7024 2115
Size: 354 1035 294 330 2896 4698
Fragments arranged by size:
4698 2896 1035 354 330 294
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2066 2271 2631 3380 5516 5584 6359
Size: 234 42 205 360 749 2136 68 775
Cuts at: 6359 6529 7668 8824 1790
Size: 170 1139 1156 2573
Fragments arranged by size:
2573 2136 1156 1139 775 749 360 234
205 170 68 42
BmgBI CAC'GTC
Cuts at: 1318 3253 1318
Size: 1935 7672
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3222 7338 8296
Size: 416 53 83 399 24 1805 4116 958
Cuts at: 8296 442
Size: 1753
Fragments arranged by size:
4116 1805 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2841 3493 4252 5296 2841
Size: 652 759 1044 7152
Fragments arranged by size:
7152 1044 759 652
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2144 2373 2387 2577 2607 2702 2784
Size: 890 15 229 14 190 30 95 82
Cuts at: 2784 2841 3027 3147 3177 3231 3555 3588 3651
Size: 57 186 120 30 54 324 33 63
Cuts at: 3651 3843 4065 4193 4331 4733 5764 6544 8267
Size: 192 222 128 138 402 1031 780 1723
Cuts at: 8267 1239
Size: 2579
Fragments arranged by size:
2579 1723 1031 890 780 402 324 229
222 192 190 186 138 128 120 95
82 63 57 54 33 30 30 15
14
Bpu10I CC'TnA_GC
Cuts at: 2175 3522 4404 2175
Size: 1347 882 7378
Fragments arranged by size:
7378 1347 882
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2774 3609 4131 6090 6725 6844 7732 8597 8895
Size: 835 522 1959 635 119 888 865 298
Cuts at: 8895 2774
Size: 3486
Fragments arranged by size:
3486 1959 888 865 835 635 522 298
119
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2681 2969 3140 3704 4226 6087 8270
Size: 617 1182 288 171 564 522 1861 2183
Cuts at: 8270 882
Size: 2219
Fragments arranged by size:
2219 2183 1861 1182 617 564 522 288
171
BsaAI yAC'GTr
Cuts at: 493 1204 7398 493
Size: 711 6194 2702
Fragments arranged by size:
6194 2702 711
BsaBI GATnn'nnATC
Cuts at: 4950 9601 4950
Size: 4651 4956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7414 7796 8431 275
Size: 53 83 186 936 5881 382 635 1451
Fragments arranged by size:
5881 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3451 6498 8040 8438 8712 8859 9270 3451
Size: 3047 1542 398 274 147 411 3788
Fragments arranged by size:
3788 3047 1542 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3518 3548 3649 3679 3709
Size: 30 165 30 2115 30 101 30 30
Cuts at: 3709 3739 3841 3871 4006 4036 4057 4060 4087
Size: 30 102 30 135 30 21 3 27
Cuts at: 4087 4090 4309 4339 4460 4490 1178
Size: 3 219 30 121 30 6295
Fragments arranged by size:
6295 2115 219 165 135 121 102 101
30 30 30 30 30 30 30 30
30 30 27 21 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2185 3011 3539 4065 4068 4244 4317 5288
Size: 999 826 528 526 3 176 73 971
Cuts at: 5288 6899 6902 1186
Size: 1611 3 3891
Fragments arranged by size:
3891 1611 999 971 826 528 526 176
73 3 3
BseYI C'CCAG_C
Cuts at: 2236 2731 2788 2967 3138 3486 3559 3815 6287
Size: 495 57 179 171 348 73 256 2472
Cuts at: 6287 6438 8810 9306 2236
Size: 151 2372 496 2537
Fragments arranged by size:
2537 2472 2372 496 495 348 256 179
171 151 73 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3275 3303 4439 4440 1163
Size: 573 1539 28 1136 1 6330
Fragments arranged by size:
6330 1539 1136 573 28 1
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7057 7818 8846 1540
Size: 275 186 5056 761 1028 2301
Fragments arranged by size:
5056 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4503 6671 7668 7753 8824 4503
Size: 2168 997 85 1071 5286
Fragments arranged by size:
5286 2168 1071 997 85
BsmI GAATG_Cn'
Cuts at: 3610 3967 4132 4896 6296 7118 7211 3610
Size: 357 165 764 1400 822 93 6006
Fragments arranged by size:
6006 1400 822 764 357 165 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6562 6562
Size: 9607
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2066 2181 2271 2631 2670 3380
Size: 399 234 42 115 90 360 39 710
Cuts at: 3380 4503 4584 5280 5516 5584 6359 6378 6529
Size: 1123 81 696 236 68 775 19 151
Cuts at: 6529 6671 6926 7668 7753 8824 1391
Size: 142 255 742 85 1071 2174
Fragments arranged by size:
2174 1123 1071 775 742 710 696 399
360 255 236 234 151 142 115 90
85 81 68 42 39 19
BspEI T'CCGG_A
Cuts at: 3451 6498 9270 3451
Size: 3047 2772 3788
Fragments arranged by size:
3788 3047 2772
BspHI T'CATG_A
Cuts at: 2836 3172 5031 5214 7496 9226 2836
Size: 336 1859 183 2282 1730 3217
Fragments arranged by size:
3217 2282 1859 1730 336 183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2914 4408 4517 6962 844
Size: 2070 1494 109 2445 3489
Fragments arranged by size:
3489 2445 2070 1494 109
BsrBI CCG'CTC
Cuts at: 1394 1823 2015 2143 7494 1394
Size: 429 192 128 5351 3507
Fragments arranged by size:
5351 3507 429 192 128
BsrDI GCAATG_nn'
Cuts at: 65 1248 4849 5137 6395 6483 8102 8276 65
Size: 1183 3601 288 1258 88 1619 174 1396
Fragments arranged by size:
3601 1619 1396 1258 1183 288 174 88
BsrFI r'CCGG_y
Cuts at: 1700 1810 5484 8251 8438 1700
Size: 110 3674 2767 187 2869
Fragments arranged by size:
3674 2869 2767 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9607
BssHII G'CGCG_C
Cuts at: 1925 5766 1925
Size: 3841 5766
BssSI C'ACGA_G
Cuts at: 7667 8679 7667
Size: 1012 8595
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4891 6188 794
Size: 4097 1297 4213
Fragments arranged by size:
4213 4097 1297
BstBI TT'CG_AA
Cuts at: 8411 8411
Size: 9607
BstEII G'GTnAC_C
Cuts at: 1184 2920 3197 1184
Size: 1736 277 7594
Fragments arranged by size:
7594 1736 277
BstYI r'GATC_y
Cuts at: 1972 2461 3646 4945 6813 6937 7304 7689 7706
Size: 489 1185 1299 1868 124 367 385 17
Cuts at: 7706 9158 9244 9256 9602 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1868 1452 1299 1185 489 385 367
346 124 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3927 4092 6273 3927
Size: 165 2181 7261
Fragments arranged by size:
7261 2181 165
BtgI C'CryG_G
Cuts at: 513 1065 2066 2207 2246 4224 513
Size: 552 1001 141 39 1978 5896
Fragments arranged by size:
5896 1978 1001 552 141 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8868
Fragments arranged by size:
8868 683 56
BtsI GCAGTG_nn'
Cuts at: 5296 5601 7123 7917 7937 5296
Size: 305 1522 794 20 6966
Fragments arranged by size:
6966 1522 794 305 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9607
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2075 2110 529
Size: 35 1511 35 8026
Fragments arranged by size:
8026 1511 35 35
DraI TTT'AAA
Cuts at: 2403 4960 5098 6911 2403
Size: 2557 138 1813 5099
Fragments arranged by size:
5099 2557 1813 138
DraIII CAC_nnn'GTG
Cuts at: 3230 3230
Size: 9607
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8614 809
Size: 507 7298 1802
Fragments arranged by size:
7298 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 7054 7943 9572
Size: 54 1425 50 275 18 5224 889 1629
Cuts at: 9572 8
Size: 43
Fragments arranged by size:
5224 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 7054 1537
Size: 275 5242 4090
Fragments arranged by size:
5242 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2716 3893 6041 7537 1353
Size: 1363 1177 2148 1496 3423
Fragments arranged by size:
3423 2148 1496 1363 1177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4550 8194 8568 8714 387
Size: 1064 138 2961 3644 374 146 1280
Fragments arranged by size:
3644 2961 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4501 4501
Size: 9607
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2144 2373 2387 2474 2577 2607
Size: 393 497 15 229 14 87 103 30
Cuts at: 2607 2702 2784 2841 2916 3027 3147 3177 3231
Size: 95 82 57 75 111 120 30 54
Cuts at: 3231 3555 3588 3651 3843 4065 4095 4193 4331
Size: 324 33 63 192 222 30 98 138
Cuts at: 4331 4389 4733 5199 5764 6123 6544 7670 8267
Size: 58 344 466 565 359 421 1126 597
Cuts at: 8267 9054 9322 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1126 787 597 565 497 466 421
393 359 344 324 268 229 222 192
138 120 111 103 98 95 87 82
75 63 58 57 54 33 30 30
30 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 4080 5801 6069 4080
Size: 1721 268 7618
Fragments arranged by size:
7618 1721 268
EcoO109I rG'GnC_Cy
Cuts at: 2062 2137 2267 2268 2627 2628 2785 3028 3376
Size: 75 130 1 359 1 157 243 348
Cuts at: 3376 3556 5669 6525 2062
Size: 180 2113 856 5144
Fragments arranged by size:
5144 2113 856 359 348 243 180 157
130 75 1 1
EcoRI G'AATT_C
Cuts at: 6916 7294 6916
Size: 378 9229
FspI TGC'GCA
Cuts at: 8113 8113
Size: 9607
HaeII r_GCGC'y
Cuts at: 1678 8754 1678
Size: 7076 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2168 2200 2690
Size: 32 135 32 427 32 137 32 490
Cuts at: 2690 2722 2939 2971 3110 3139 3142 3171 3518
Size: 32 217 32 139 29 3 29 347
Cuts at: 3518 3550 3703 3735 3837 3869 4057 4089 4225
Size: 32 153 32 102 32 188 32 136
Cuts at: 4225 4251 4257 4283 4309 4339 4341 4371 4511
Size: 26 6 26 26 30 2 30 140
Cuts at: 4511 4543 5393 5425 6000 6032 6805 6837 8248
Size: 32 850 32 575 32 773 32 1411
Cuts at: 8248 8280 8322 8354 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1411 850 773 575 490 427 347
217 188 153 140 139 137 136 135
102 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 30 30 29 29 26 26
26 6 3 2
HincII GTy'rAC
Cuts at: 669 1657 1938 5395 6951 7197 669
Size: 988 281 3457 1556 246 3079
Fragments arranged by size:
3457 3079 1556 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2871 6382 6581 748
Size: 2123 3511 199 3774
Fragments arranged by size:
3774 3511 2123 199
HpaI GTT'AAC
Cuts at: 5395 7197 5395
Size: 1802 7805
KpnI G_GTAC'C
Cuts at: 6932 6932
Size: 9607
MfeI C'AATT_G
Cuts at: 3033 7206 3033
Size: 4173 5434
MluI A'CGCG_T
Cuts at: 9483 9483
Size: 9607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2456 2623 3158 3506 3722 3800 3859 4244
Size: 1151 167 535 348 216 78 59 385
Cuts at: 4244 4652 8721 8905 1305
Size: 408 4069 184 2007
Fragments arranged by size:
4069 2007 1151 535 408 385 348 216
184 167 78 59
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7785
Fragments arranged by size:
7785 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2032 5030 5219 6224 7565 7924 8083
Size: 648 866 2998 189 1005 1341 359 159
Cuts at: 8083 518
Size: 2042
Fragments arranged by size:
2998 2042 1341 1005 866 648 359 189
159
MspA1I CmG'CkG
Cuts at: 1606 2050 2149 2351 3503 3521 6287 6445 7023
Size: 444 99 202 1152 18 2766 158 578
Cuts at: 7023 7700 8848 9093 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2766 2120 1152 1148 677 578 444 245
202 158 99 18
NcoI C'CATG_G
Cuts at: 513 1065 2066 513
Size: 552 1001 8054
Fragments arranged by size:
8054 1001 552
NdeI CA'TA_TG
Cuts at: 387 4493 5073 387
Size: 4106 580 4921
Fragments arranged by size:
4921 4106 580
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9607
NotI GC'GGCC_GC
Cuts at: 7054 7054
Size: 9607
NspI r_CATG'y
Cuts at: 1132 1229 5154 6229 6965 8510 1132
Size: 97 3925 1075 736 1545 2229
Fragments arranged by size:
3925 2229 1545 1075 736 97
PasI CC'CwG_GG
Cuts at: 1348 2164 1348
Size: 816 8791
PciI A'CATG_T
Cuts at: 8506 8506
Size: 9607
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6731 1108
Size: 5623 3984
PfoI T'CCnGG_A
Cuts at: 2331 4081 6560 6724 2331
Size: 1750 2479 164 5214
Fragments arranged by size:
5214 2479 1750 164
PmeI GTTT'AAAC
Cuts at: 6911 6911
Size: 9607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3975 4007 7676 7708 9189 9221 3975
Size: 32 3669 32 1481 32 4361
Fragments arranged by size:
4361 3669 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2137
Size: 9607
PshAI GACnn'nnGTC
Cuts at: 1613 4119 4370 6099 1613
Size: 2506 251 1729 5121
Fragments arranged by size:
5121 2506 1729 251
PsiI TTA'TAA
Cuts at: 4829 7177 4829
Size: 2348 7259
PspOMI G'GGCC_C
Cuts at: 2062 2267 2627 3376 5512 6525 2062
Size: 205 360 749 2136 1013 5144
Fragments arranged by size:
5144 2136 1013 749 360 205
PspXI vC'TCGA_Gb
Cuts at: 3981 3981
Size: 9607
PstI C_TGCA'G
Cuts at: 830 2911 3502 6043 6450 6959 830
Size: 2081 591 2541 407 509 3478
Fragments arranged by size:
3478 2541 2081 591 509 407
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9607
PvuII CAG'CTG
Cuts at: 2149 2351 3503 6445 2149
Size: 202 1152 2942 5311
Fragments arranged by size:
5311 2942 1152 202
SacI G_AGCT'C
Cuts at: 4503 4503
Size: 9607
SalI G'TCGA_C
Cuts at: 1936 6949 1936
Size: 5013 4594
SapI GCTCTTCn'nnn_
Cuts at: 6041 6041
Size: 9607
SbfI CC_TGCA'GG
Cuts at: 6959 6959
Size: 9607
ScaI AGT'ACT
Cuts at: 7855 9567 7855
Size: 1712 7895
SexAI A'CCwGG_T
Cuts at: 3781 3951 3781
Size: 170 9437
SfcI C'TryA_G
Cuts at: 826 1045 2907 3498 3906 4053 4068 4200 4284
Size: 219 1862 591 408 147 15 132 84
Cuts at: 4284 4302 4320 5190 5472 6039 6446 6955 8090
Size: 18 18 870 282 567 407 509 1135
Cuts at: 8090 8771 8962 826
Size: 681 191 1471
Fragments arranged by size:
1862 1471 1135 870 681 591 567 509
408 407 282 219 191 147 132 84
18 18 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9607
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9607
SmaI CCC'GGG
Cuts at: 1872 6934 1872
Size: 5062 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2753 3624 3981 4146 5018 6088
Size: 197 926 810 871 357 165 872 1070
Cuts at: 6088 6105 6704 6859 7711 8612 8874 820
Size: 17 599 155 852 901 262 1553
Fragments arranged by size:
1553 1070 926 901 872 871 852 810
599 357 262 197 165 155 17
SnaBI TAC'GTA
Cuts at: 493 7398 493
Size: 6905 2702
SpeI A'CTAG_T
Cuts at: 152 2101 152
Size: 1949 7658
SphI G_CATG'C
Cuts at: 1132 6965 1132
Size: 5833 3774
SspI AAT'ATT
Cuts at: 5 52 2555 7531 5
Size: 47 2503 4976 2081
Fragments arranged by size:
4976 2503 2081 47
StuI AGG'CCT
Cuts at: 1517 4475 4737 4880 1517
Size: 2958 262 143 6244
Fragments arranged by size:
6244 2958 262 143
StyI C'CwwG_G
Cuts at: 513 1065 2066 4621 5516 5636 5760 5961 7046
Size: 552 1001 2555 895 120 124 201 1085
Cuts at: 7046 513
Size: 3074
Fragments arranged by size:
3074 2555 1085 1001 895 552 201 124
120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3241 3829 4451 4604 4691 5732 5974
Size: 1255 1661 588 622 153 87 1041 242
Cuts at: 5974 7634 7651 7804 7989 8434 325
Size: 1660 17 153 185 445 1498
Fragments arranged by size:
1661 1660 1498 1255 1041 622 588 445
242 185 153 153 87 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2561 5357 7853
Size: 274 80 33 51 1443 584 2796 2496
Cuts at: 7853 9565 96
Size: 1712 138
Fragments arranged by size:
2796 2496 1712 1443 584 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2498 2530 4460 4492 5158 5190 5825 5857 9567
Size: 32 1930 32 666 32 635 32 3710
Cuts at: 9567 9599 2498
Size: 32 2506
Fragments arranged by size:
3710 2506 1930 666 635 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9607
XbaI T'CTAG_A
Cuts at: 2662 4981 6943 2662
Size: 2319 1962 5326
Fragments arranged by size:
5326 2319 1962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2244 2472 2244
Size: 228 9379
XhoI C'TCGA_G
Cuts at: 1943 3981 1943
Size: 2038 7569
XmaI C'CCGG_G
Cuts at: 1870 6932 1870
Size: 5062 4545
XmnI GAAnn'nnTTC
Cuts at: 5753 7736 5753
Size: 1983 7624
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7415 276
Size: 53 83 186 936 5881 2468
Fragments arranged by size:
5881 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbeI BfrBI BsiWI
BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI