Restriction map for
pFN21AA0561
(Circular) (Six-base) MAPSORT of: pFN21AA0561.seq Check: 6143 from: 1 to: 8632
pFN21AA0561 8632 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2898 3610 5647 2898
Size: 712 2037 5883
Fragments arranged by size:
5883 2037 712
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4932 5061 6442 278
Size: 53 83 186 936 3396 129 1381 2468
Fragments arranged by size:
3396 2468 1381 936 186 129 83 53
AccI GT'mk_AC
Cuts at: 1937 3147 4125 4911 4932 5975 1937
Size: 1210 978 786 21 1043 4594
Fragments arranged by size:
4594 1210 1043 978 786 21
Acc65I G'GTAC_C
Cuts at: 5953 5953
Size: 8632
AclI AA'CG_TT
Cuts at: 1563 4024 6759 7132 1563
Size: 2461 2735 373 3063
Fragments arranged by size:
3063 2735 2461 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3392 4168 4873 5096 6695 8079 8347 1632
Size: 1760 776 705 223 1599 1384 268 1917
Fragments arranged by size:
1917 1760 1599 1384 776 705 268 223
AfeI AGC'GCT
Cuts at: 3179 3179
Size: 8632
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8435
AflIII A'CryG_T
Cuts at: 1203 2526 4013 7531 8508 1203
Size: 1323 1487 3518 977 1327
Fragments arranged by size:
3518 1487 1327 1323 977
AgeI A'CCGG_T
Cuts at: 4110 7463 4110
Size: 3353 5279
AhdI GACnn_n'nnGTC
Cuts at: 7361 7361
Size: 8632
AleI CACnn'nnGTG
Cuts at: 1166 4472 5864 1166
Size: 3306 1392 3934
Fragments arranged by size:
3934 3306 1392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3198 1184
Size: 2014 6618
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4778 4810 4778
Size: 32 8600
AlwNI CAG_nnn'CTG
Cuts at: 3041 4409 4625 4697 5384 5459 6375 7947 3041
Size: 1368 216 72 687 75 916 1572 3726
Fragments arranged by size:
3726 1572 1368 916 687 216 75 72
ApaI G_GGCC'C
Cuts at: 3386 4610 5141 5919 3386
Size: 1224 531 778 6099
Fragments arranged by size:
6099 1224 778 531
ApaLI G'TGCA_C
Cuts at: 3464 4940 5048 6689 7845 3464
Size: 1476 108 1641 1156 4251
Fragments arranged by size:
4251 1641 1476 1156 108
ApoI r'AATT_y
Cuts at: 1493 2194 2218 2879 5941 6167 6319 8428 1493
Size: 701 24 661 3062 226 152 2109 1697
Fragments arranged by size:
3062 2109 1697 701 661 226 152 24
AseI AT'TA_AT
Cuts at: 160 1032 7186 160
Size: 872 6154 1606
Fragments arranged by size:
6154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8632
AvaI C'yCGr_G
Cuts at: 1870 1943 2331 2476 2670 3600 3650 3758 4310
Size: 73 388 145 194 930 50 108 552
Cuts at: 4310 4489 5428 5621 5912 5957 1870
Size: 179 939 193 291 45 4545
Fragments arranged by size:
4545 939 930 552 388 291 194 193
179 145 108 73 50 45
BamHI G'GATC_C
Cuts at: 4280 5962 6329 4280
Size: 1682 367 6583
Fragments arranged by size:
6583 1682 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2028 2606 3001 3159 3315
Size: 325 217 627 241 578 395 158 156
Cuts at: 3315 3893 4550 5625 5953 7408 618
Size: 578 657 1075 328 1455 1842
Fragments arranged by size:
1842 1455 1075 657 627 578 578 395
328 325 241 217 158 156
BanII G_rGCy'C
Cuts at: 1391 2040 3386 3869 4043 4610 4760 5141 5435
Size: 649 1346 483 174 567 150 381 294
Cuts at: 5435 5748 5919 5951 1391
Size: 313 171 32 4072
Fragments arranged by size:
4072 1346 649 567 483 381 313 294
174 171 150 32
BbeI G_GCGC'C
Cuts at: 2032 2610 3319 4554 2032
Size: 578 709 1235 6110
Fragments arranged by size:
6110 1235 709 578
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4197 4423 928
Size: 971 2298 226 5137
Fragments arranged by size:
5137 2298 971 226
BbvCI CC'TCA_GC
Cuts at: 2904 4259 2904
Size: 1355 7277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2005 2039 4493 4527 4528 4562 6823
Size: 34 68 34 2454 34 1 34 2261
Cuts at: 6823 6857 1903
Size: 34 3678
Fragments arranged by size:
3678 2454 2261 68 34 34 34 34
34 1
BciVI GTATCCnnnnn_n'
Cuts at: 6524 7734 6524
Size: 1210 7422
BclI T'GATC_A
Cuts at: 1548 2823 1548
Size: 1275 7357
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2237 2615 2845
Size: 107 122 71 631 605 565 378 230
Cuts at: 2845 3140 4038 4413 4605 4985 5325 7243 136
Size: 295 898 375 192 380 340 1918 1525
Fragments arranged by size:
1918 1525 898 631 605 565 380 378
375 340 295 230 192 122 107 71
BglII A'GATC_T
Cuts at: 5238 8627 5238
Size: 3389 5243
BlpI GC'TnA_GC
Cuts at: 2051 6049 2051
Size: 3998 4634
Bme1580I G_kGCm'C
Cuts at: 1790 3386 3468 3887 3975 4610 4686 4944 5052
Size: 1596 82 419 88 635 76 258 108
Cuts at: 5052 5141 5628 5690 5919 6693 7849 1790
Size: 89 487 62 229 774 1156 2573
Fragments arranged by size:
2573 1596 1156 774 635 487 419 258
229 108 89 88 82 76 62
BmgBI CAC'GTC
Cuts at: 1318 2072 4948 1318
Size: 754 2876 5002
Fragments arranged by size:
5002 2876 754
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3946 4084 4466
Size: 416 53 83 399 24 2529 138 382
Cuts at: 4466 5859 6363 7321 442
Size: 1393 504 958 1753
Fragments arranged by size:
2529 1753 1393 958 504 416 399 382
138 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8632
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5140 5140
Size: 8632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2024 2762 2873 2882 2915 3011 3458 4630
Size: 785 738 111 9 33 96 447 1172
Cuts at: 4630 4975 5135 5419 7292 1239
Size: 345 160 284 1873 2579
Fragments arranged by size:
2579 1873 1172 785 738 447 345 284
160 111 96 33 9
Bpu10I CC'TnA_GC
Cuts at: 2904 4259 2904
Size: 1355 7277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6757 7622 7920 6757
Size: 865 298 7469
Fragments arranged by size:
7469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3101 3302 4049 4608 4812 5198 7295
Size: 617 1602 201 747 559 204 386 2097
Cuts at: 7295 882
Size: 2219
Fragments arranged by size:
2219 2097 1602 747 617 559 386 204
201
BsaAI yAC'GTr
Cuts at: 493 1204 2527 3343 6423 493
Size: 711 1323 816 3080 2702
Fragments arranged by size:
3080 2702 1323 816 711
BsaBI GATnn'nnATC
Cuts at: 2828 8626 2828
Size: 5798 2834
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2029 2366 2607 2631
Size: 53 83 186 936 496 337 241 24
Cuts at: 2631 3316 3597 3632 3705 3831 4551 4929 5058
Size: 685 281 35 73 126 720 378 129
Cuts at: 5058 6439 6821 7456 275
Size: 1381 382 635 1451
Fragments arranged by size:
1451 1381 936 720 685 635 496 382
378 337 281 241 186 129 126 83
73 53 35 24
BsaWI w'CCGG_w
Cuts at: 2811 4110 5285 5921 7065 7463 7737 7884 8295
Size: 1299 1175 636 1144 398 274 147 411
Cuts at: 8295 2811
Size: 3148
Fragments arranged by size:
3148 1299 1175 1144 636 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2433 2463 4171 4190 4201
Size: 30 165 30 1030 30 1708 19 11
Cuts at: 4201 4220 5330 5360 1178
Size: 19 1110 30 4450
Fragments arranged by size:
4450 1708 1110 1030 165 30 30 30
30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2798 3008 4087 4214 4633 5203 5272 5855
Size: 1612 210 1079 127 419 570 69 583
Cuts at: 5855 1186
Size: 3963
Fragments arranged by size:
3963 1612 1079 583 570 419 210 127
69
BseYI C'CCAG_C
Cuts at: 2300 2968 3034 3975 4305 4322 4618 5000 5038
Size: 668 66 941 330 17 296 382 38
Cuts at: 5038 5351 5377 5392 5417 5452 5477 5683 7835
Size: 313 26 15 25 35 25 206 2152
Cuts at: 7835 8331 2300
Size: 496 2601
Fragments arranged by size:
2601 2152 941 668 496 382 330 313
296 206 66 38 35 26 25 25
17 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2363 5246 5471 5750 5831 1163
Size: 573 627 2883 225 279 81 3964
Fragments arranged by size:
3964 2883 627 573 279 225 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2437 4588 6082 6843 7871 1540
Size: 275 186 436 2151 1494 761 1028 2301
Fragments arranged by size:
2301 2151 1494 1028 761 436 275 186
BsiHKAI G_wGCw'C
Cuts at: 3468 4944 5052 5435 5748 6693 6778 7849 3468
Size: 1476 108 383 313 945 85 1071 4251
Fragments arranged by size:
4251 1476 1071 945 383 313 108 85
BsmI GAATG_Cn'
Cuts at: 6143 6236 6143
Size: 93 8539
BsmBI CGTCTCn'nnnn_
Cuts at: 2271 2345 3416 5922 2271
Size: 74 1071 2506 4981
Fragments arranged by size:
4981 2506 1071 74
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 3386 3468 3869 3887 3975 4043
Size: 399 250 1346 82 401 18 88 68
Cuts at: 4043 4610 4686 4760 4944 5052 5141 5435 5628
Size: 567 76 74 184 108 89 294 193
Cuts at: 5628 5690 5748 5919 5951 6693 6778 7849 1391
Size: 62 58 171 32 742 85 1071 2174
Fragments arranged by size:
2174 1346 1071 742 567 401 399 294
250 193 184 171 108 89 88 85
82 76 74 68 62 58 32 18
BspEI T'CCGG_A
Cuts at: 2811 5285 8295 2811
Size: 2474 3010 3148
Fragments arranged by size:
3148 3010 2474
BspHI T'CATG_A
Cuts at: 2514 3552 4752 6521 8251 2514
Size: 1038 1200 1769 1730 2895
Fragments arranged by size:
2895 1769 1730 1200 1038
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2898 3226 3453 3610 5647 5987 844
Size: 2054 328 227 157 2037 340 3489
Fragments arranged by size:
3489 2054 2037 340 328 227 157
BsrBI CCG'CTC
Cuts at: 1394 1823 2460 2746 4315 5082 5604 5698 6519
Size: 429 637 286 1569 767 522 94 821
Cuts at: 6519 1394
Size: 3507
Fragments arranged by size:
3507 1569 821 767 637 522 429 286
94
BsrDI GCAATG_nn'
Cuts at: 65 1248 7127 7301 65
Size: 1183 5879 174 1396
Fragments arranged by size:
5879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2436 4110 4236 4583 7276 7463 1700
Size: 110 626 1674 126 347 2693 187 2869
Fragments arranged by size:
2869 2693 1674 626 347 187 126 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8632
BssSI C'ACGA_G
Cuts at: 5727 5747 6692 7704 5727
Size: 20 945 1012 6655
Fragments arranged by size:
6655 1012 945 20
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3297 794
Size: 2503 6129
BstBI TT'CG_AA
Cuts at: 7436 7436
Size: 8632
BstEII G'GTnAC_C
Cuts at: 1184 2439 1184
Size: 1255 7377
BstXI CCAn_nnnn'nTGG
Cuts at: 3674 3690 4351 4871 5113 5134 3674
Size: 16 661 520 242 21 7172
Fragments arranged by size:
7172 661 520 242 21 16
BstYI r'GATC_y
Cuts at: 1972 3228 4083 4280 5238 5962 6329 6714 6731
Size: 1256 855 197 958 724 367 385 17
Cuts at: 6731 8183 8269 8281 8627 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1256 958 855 724 385 367
346 197 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2874 2874
Size: 8632
BtgI C'CryG_G
Cuts at: 513 1065 2004 2032 3393 3699 5651 5880 513
Size: 552 939 28 1361 306 1952 229 3265
Fragments arranged by size:
3265 1952 1361 939 552 306 229 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3801 487
Size: 683 56 2575 5318
Fragments arranged by size:
5318 2575 683 56
BtsI GCAGTG_nn'
Cuts at: 4663 4675 5403 6148 6942 6962 4663
Size: 12 728 745 794 20 6333
Fragments arranged by size:
6333 794 745 728 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8597
DraI TTT'AAA
Cuts at: 5936 5936
Size: 8632
DraIII CAC_nnn'GTG
Cuts at: 3904 3904
Size: 8632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7639 809
Size: 507 6323 1802
Fragments arranged by size:
6323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2434 2682 2837
Size: 54 1425 50 275 18 604 248 155
Cuts at: 2837 2976 3070 3508 3561 3800 4146 4231 4464
Size: 139 94 438 53 239 346 85 233
Cuts at: 4464 4585 5157 5464 5530 6079 6968 8597 8
Size: 121 572 307 66 549 889 1629 43
Fragments arranged by size:
1629 1425 889 604 572 549 438 346
307 275 248 239 233 155 139 121
94 85 66 54 53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2434 4585 6079 1537
Size: 275 622 2151 1494 4090
Fragments arranged by size:
4090 2151 1494 622 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3773 4810 5072 5920 6562 1353
Size: 2420 1037 262 848 642 3423
Fragments arranged by size:
3423 2420 1037 848 642 262
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3653 5233 7219 7593 7739 387
Size: 1064 138 2064 1580 1986 374 146 1280
Fragments arranged by size:
2064 1986 1580 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5433 5746 5433
Size: 313 8319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2024 2762 2873 2882 2915 3011 3392
Size: 393 392 738 111 9 33 96 381
Cuts at: 3392 3458 4168 4630 4873 4975 5096 5135 5419
Size: 66 710 462 243 102 121 39 284
Cuts at: 5419 6695 7292 8079 8347 1239
Size: 1276 597 787 268 1524
Fragments arranged by size:
1524 1276 787 738 710 597 462 393
392 381 284 268 243 121 111 102
96 66 39 33 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2894 2978 3575 2894
Size: 84 597 7951
Fragments arranged by size:
7951 597 84
EcoO109I rG'GnC_Cy
Cuts at: 2493 2983 3817 3838 4263 4319 4406 4415 4606
Size: 490 834 21 425 56 87 9 191
Cuts at: 4606 5005 5138 2493
Size: 399 133 5987
Fragments arranged by size:
5987 834 490 425 399 191 133 87
56 21 9
EcoRI G'AATT_C
Cuts at: 5941 6319 5941
Size: 378 8254
EcoRV GAT'ATC
Cuts at: 5094 5094
Size: 8632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4390 4903 4390
Size: 513 8119
FspI TGC'GCA
Cuts at: 7138 7138
Size: 8632
HaeII r_GCGC'y
Cuts at: 1678 2032 2610 2621 3181 3319 3437 4554 5782
Size: 354 578 11 560 138 118 1117 1228
Cuts at: 5782 7779 1678
Size: 1997 2531
Fragments arranged by size:
2531 1997 1228 1117 578 560 354 138
118 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2244 2276 3594 3626 4055
Size: 32 135 32 672 32 1318 32 429
Cuts at: 4055 4087 5179 5197 5211 5229 7273 7305 7347
Size: 32 1092 18 14 18 2044 32 42
Cuts at: 7347 7379 1373
Size: 32 2626
Fragments arranged by size:
2626 2044 1318 1092 672 429 135 42
32 32 32 32 32 32 32 18
18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 5976 6222 669
Size: 988 281 4038 246 3079
Fragments arranged by size:
4038 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5504 748
Size: 4756 3876
HpaI GTT'AAC
Cuts at: 6222 6222
Size: 8632
KasI G'GCGC_C
Cuts at: 2028 2606 3315 4550 2028
Size: 578 709 1235 6110
Fragments arranged by size:
6110 1235 709 578
KpnI G_GTAC'C
Cuts at: 5957 5957
Size: 8632
MfeI C'AATT_G
Cuts at: 6231 6231
Size: 8632
MluI A'CGCG_T
Cuts at: 8508 8508
Size: 8632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2476 3976 7746 7930 1305
Size: 1171 1500 3770 184 2007
Fragments arranged by size:
3770 2007 1500 1171 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2684 2978 3510 3563 3802
Size: 54 1425 343 852 294 532 53 239
Cuts at: 3802 5159 5466 10
Size: 1357 307 3176
Fragments arranged by size:
3176 1425 1357 852 532 343 307 294
239 54 53
MslI CAynn'nnrTG
Cuts at: 518 1166 3453 3557 4472 5132 5864 6590 6949
Size: 648 2287 104 915 660 732 726 359
Cuts at: 6949 7108 518
Size: 159 2042
Fragments arranged by size:
2287 2042 915 732 726 660 648 359
159 104
MspA1I CmG'CkG
Cuts at: 1606 2034 2085 2968 3388 3871 4463 4511 4650
Size: 428 51 883 420 483 592 48 139
Cuts at: 4650 4740 5456 5569 5653 5675 6048 6725 7873
Size: 90 716 113 84 22 373 677 1148
Cuts at: 7873 8118 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 883 716 677 592 483 428
420 373 245 139 113 90 84 51
48 22
NaeI GCC'GGC
Cuts at: 4238 4585 4238
Size: 347 8285
NarI GG'CG_CC
Cuts at: 2029 2607 3316 4551 2029
Size: 578 709 1235 6110
Fragments arranged by size:
6110 1235 709 578
NcoI C'CATG_G
Cuts at: 513 1065 2004 3699 513
Size: 552 939 1695 5446
Fragments arranged by size:
5446 1695 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8632
NgoMIV G'CCGG_C
Cuts at: 4236 4583 4236
Size: 347 8285
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8632
NotI GC'GGCC_GC
Cuts at: 6079 6079
Size: 8632
NspI r_CATG'y
Cuts at: 1132 1229 3295 5990 7535 1132
Size: 97 2066 2695 1545 2229
Fragments arranged by size:
2695 2229 2066 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8632
PciI A'CATG_T
Cuts at: 7531 7531
Size: 8632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2893 1108
Size: 1785 6847
PfoI T'CCnGG_A
Cuts at: 4265 4265
Size: 8632
PmeI GTTT'AAAC
Cuts at: 5936 5936
Size: 8632
PmlI CAC'GTG
Cuts at: 2527 2527
Size: 8632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6701 6733 8214 8246 6701
Size: 32 1481 32 7087
Fragments arranged by size:
7087 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2493 2983 3838 4263 4319 5005 2493
Size: 490 855 425 56 686 6120
Fragments arranged by size:
6120 855 686 490 425 56
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8632
PsiI TTA'TAA
Cuts at: 6202 6202
Size: 8632
PspOMI G'GGCC_C
Cuts at: 3382 4606 5137 5915 3382
Size: 1224 531 778 6099
Fragments arranged by size:
6099 1224 778 531
PspXI vC'TCGA_Gb
Cuts at: 4489 4489
Size: 8632
PstI C_TGCA'G
Cuts at: 830 2580 2757 4414 5984 830
Size: 1750 177 1657 1570 3478
Fragments arranged by size:
3478 1750 1657 1570 177
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8632
PvuII CAG'CTG
Cuts at: 2968 4511 5456 5675 2968
Size: 1543 945 219 5925
Fragments arranged by size:
5925 1543 945 219
SacI G_AGCT'C
Cuts at: 5435 5748 5435
Size: 313 8319
SacII CC_GC'GG
Cuts at: 2035 5654 2035
Size: 3619 5013
SalI G'TCGA_C
Cuts at: 1936 5974 1936
Size: 4038 4594
SanDI GG'GwC_CC
Cuts at: 4319 4319
Size: 8632
SapI GCTCTTCn'nnn_
Cuts at: 5072 5072
Size: 8632
SbfI CC_TGCA'GG
Cuts at: 4414 5984 4414
Size: 1570 7062
ScaI AGT'ACT
Cuts at: 6880 8592 6880
Size: 1712 6920
SexAI A'CCwGG_T
Cuts at: 3150 3848 3150
Size: 698 7934
SfcI C'TryA_G
Cuts at: 826 1045 2576 2753 4126 4410 5980 7115 7796
Size: 219 1531 177 1373 284 1570 1135 681
Cuts at: 7796 7987 826
Size: 191 1471
Fragments arranged by size:
1570 1531 1471 1373 1135 681 284 219
191 177
SfiI GGCCn_nnn'nGGCC
Cuts at: 4413 4413
Size: 8632
SfoI GGC'GCC
Cuts at: 2030 2608 3317 4552 2030
Size: 578 709 1235 6110
Fragments arranged by size:
6110 1235 709 578
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8632
SmaI CCC'GGG
Cuts at: 1872 2333 2478 5623 5959 1872
Size: 461 145 3145 336 4545
Fragments arranged by size:
4545 3145 461 336 145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4489 6736 7637 7899 820
Size: 197 926 2546 2247 901 262 1553
Fragments arranged by size:
2546 2247 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6423 493
Size: 5930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8632
SphI G_CATG'C
Cuts at: 1132 5990 1132
Size: 4858 3774
SrfI GCCC'GGGC
Cuts at: 2478 5623 2478
Size: 3145 5487
SspI AAT'ATT
Cuts at: 5 52 6556 5
Size: 47 6504 2081
Fragments arranged by size:
6504 2081 47
StuI AGG'CCT
Cuts at: 1517 4573 1517
Size: 3056 5576
StyI C'CwwG_G
Cuts at: 513 1065 2004 2178 2547 2926 2979 3699 4568
Size: 552 939 174 369 379 53 720 869
Cuts at: 4568 4600 5160 6071 513
Size: 32 560 911 3074
Fragments arranged by size:
3074 939 911 869 720 560 552 379
369 174 53 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2143 2314 3761 3911 4907 5030 5351
Size: 1255 563 171 1447 150 996 123 321
Cuts at: 5351 5431 6659 6676 6829 7014 7459 325
Size: 80 1228 17 153 185 445 1498
Fragments arranged by size:
1498 1447 1255 1228 996 563 445 321
185 171 153 150 123 80 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2952 3411 3639
Size: 274 80 33 51 1443 975 459 228
Cuts at: 3639 6878 8590 96
Size: 3239 1712 138
Fragments arranged by size:
3239 1712 1443 975 459 274 228 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2372 2404 2433 2465 4171 4190 4203 4222 5330
Size: 32 29 32 1706 19 13 19 1108
Cuts at: 5330 5362 8592 8624 2372
Size: 32 3230 32 2380
Fragments arranged by size:
3230 2380 1706 1108 32 32 32 32
29 19 19 13
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8632
XbaI T'CTAG_A
Cuts at: 5968 5968
Size: 8632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2560 2986 3690 5167 2560
Size: 426 704 1477 6025
Fragments arranged by size:
6025 1477 704 426
XhoI C'TCGA_G
Cuts at: 1943 4489 1943
Size: 2546 6086
XmaI C'CCGG_G
Cuts at: 1870 2331 2476 5621 5957 1870
Size: 461 145 3145 336 4545
Fragments arranged by size:
4545 3145 461 336 145
XmnI GAAnn'nnTTC
Cuts at: 6761 6761
Size: 8632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4930 5059 6440 276
Size: 53 83 186 936 3396 129 1381 2468
Fragments arranged by size:
3396 2468 1381 936 186 129 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI FspAI
NruI NsiI PacI PsrI RsrII SwaI