Restriction map for
pFN21AA0556
(Circular) (Six-base) MAPSORT of: pFN21AA0556.seq Check: 7069 from: 1 to: 9559
pFN21AA0556 9559 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5745 5745
Size: 9559
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6369 7369 278
Size: 53 83 186 936 4833 1000 2468
Fragments arranged by size:
4833 2468 1000 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4952 6902 1937
Size: 3015 1950 4594
Fragments arranged by size:
4594 3015 1950
Acc65I G'GTAC_C
Cuts at: 6880 6880
Size: 9559
AclI AA'CG_TT
Cuts at: 1563 7686 8059 1563
Size: 6123 373 3063
Fragments arranged by size:
6123 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 2367 5624 7622 9006 9274 1632
Size: 482 253 3257 1998 1384 268 1917
Fragments arranged by size:
3257 1998 1917 1384 482 268 253
AfeI AGC'GCT
Cuts at: 4931 4931
Size: 9559
AflII C'TTAA_G
Cuts at: 820 1017 2356 820
Size: 197 1339 8023
Fragments arranged by size:
8023 1339 197
AflIII A'CryG_T
Cuts at: 1203 6423 6632 8458 9435 1203
Size: 5220 209 1826 977 1327
Fragments arranged by size:
5220 1826 1327 977 209
AgeI A'CCGG_T
Cuts at: 5017 8390 5017
Size: 3373 6186
AhdI GACnn_n'nnGTC
Cuts at: 4078 4636 4948 4973 8288 4078
Size: 558 312 25 3315 5349
Fragments arranged by size:
5349 3315 558 312 25
AleI CACnn'nnGTG
Cuts at: 1166 2780 1166
Size: 1614 7945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9559
AlwNI CAG_nnn'CTG
Cuts at: 2020 2438 2548 4889 5725 6118 7302 8874 2020
Size: 418 110 2341 836 393 1184 1572 2705
Fragments arranged by size:
2705 2341 1572 1184 836 418 393 110
ApaLI G'TGCA_C
Cuts at: 7616 8772 7616
Size: 1156 8403
ApoI r'AATT_y
Cuts at: 1493 2792 2830 4051 5226 5647 6868 7094 7246
Size: 1299 38 1221 1175 421 1221 226 152
Cuts at: 7246 9355 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1299 1221 1221 1175 421 226
152 38
AseI AT'TA_AT
Cuts at: 160 1032 8113 160
Size: 872 7081 1606
Fragments arranged by size:
7081 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9559
AvaI C'yCGr_G
Cuts at: 1870 1943 4247 4337 4762 5402 5780 5960 6884
Size: 73 2304 90 425 640 378 180 924
Cuts at: 6884 1870
Size: 4545
Fragments arranged by size:
4545 2304 924 640 425 378 180 90
73
BamHI G'GATC_C
Cuts at: 5556 6588 6889 7256 5556
Size: 1032 301 367 7859
Fragments arranged by size:
7859 1032 367 301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2228 2406 3761 3929 3947
Size: 325 217 627 441 178 1355 168 18
Cuts at: 3947 5825 6181 6880 8335 618
Size: 1878 356 699 1455 1842
Fragments arranged by size:
1878 1842 1455 1355 699 627 441 356
325 217 178 168 18
BanII G_rGCy'C
Cuts at: 1391 4194 4574 4709 4761 4785 5353 5574 5688
Size: 2803 380 135 52 24 568 221 114
Cuts at: 5688 5778 6112 6642 6878 1391
Size: 90 334 530 236 4072
Fragments arranged by size:
4072 2803 568 530 380 334 236 221
135 114 90 52 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2637 4632 4635 928
Size: 971 738 1995 3 5852
Fragments arranged by size:
5852 1995 971 738 3
BbvCI CC'TCA_GC
Cuts at: 2859 3080 2859
Size: 221 9338
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6837 6871 7750 7784 1903
Size: 34 4900 34 879 34 3678
Fragments arranged by size:
4900 3678 879 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5244 7451 8661 5244
Size: 2207 1210 6142
Fragments arranged by size:
6142 2207 1210
BclI T'GATC_A
Cuts at: 1548 5220 6501 1548
Size: 3672 1281 4606
Fragments arranged by size:
4606 3672 1281
BfrBI ATG'CAT
Cuts at: 5140 5140
Size: 9559
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6261 8170 136
Size: 107 122 71 631 605 4589 1909 1525
Fragments arranged by size:
4589 1909 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5745 9554 5745
Size: 3809 5750
BlpI GC'TnA_GC
Cuts at: 6111 6976 6111
Size: 865 8694
Bme1580I G_kGCm'C
Cuts at: 1790 4175 6651 7620 8776 1790
Size: 2385 2476 969 1156 2573
Fragments arranged by size:
2573 2476 2385 1156 969
BmgBI CAC'GTC
Cuts at: 1318 5995 1318
Size: 4677 4882
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2376 3472 4078
Size: 416 53 83 399 24 959 1096 606
Cuts at: 4078 4134 5293 7290 8248 442
Size: 56 1159 1997 958 1753
Fragments arranged by size:
1997 1753 1159 1096 959 958 606 416
399 83 56 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9559
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 4210 2635
Size: 1575 7984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2266 2495 2748 2926 3179 3302 3641
Size: 971 56 229 253 178 253 123 339
Cuts at: 3641 4419 4896 6176 6287 6368 8219 1239
Size: 778 477 1280 111 81 1851 2579
Fragments arranged by size:
2579 1851 1280 971 778 477 339 253
253 229 178 123 111 81 56
Bpu10I CC'TnA_GC
Cuts at: 2076 2649 2859 2883 3080 2076
Size: 573 210 24 197 8555
Fragments arranged by size:
8555 573 210 197 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3200 4558 6063 7684 8549 8847 3200
Size: 1358 1505 1621 865 298 3912
Fragments arranged by size:
3912 1621 1505 1358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2959 3167 3491 3977 4397 4471 8222
Size: 617 1460 208 324 486 420 74 3751
Cuts at: 8222 882
Size: 2219
Fragments arranged by size:
3751 2219 1460 617 486 420 324 208
74
BsaAI yAC'GTr
Cuts at: 493 1204 3439 7350 493
Size: 711 2235 3911 2702
Fragments arranged by size:
3911 2702 2235 711
BsaBI GATnn'nnATC
Cuts at: 3747 9553 3747
Size: 5806 3753
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4649 6366 7366 7748
Size: 53 83 186 936 3116 1717 1000 382
Cuts at: 7748 8383 275
Size: 635 1451
Fragments arranged by size:
3116 1717 1451 1000 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2142 3555 5017 6042 7992 8390 8664 8811 9222
Size: 1413 1462 1025 1950 398 274 147 411
Cuts at: 9222 2142
Size: 2479
Fragments arranged by size:
2479 1950 1462 1413 1025 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2921 2951 3440 3470 1178
Size: 30 165 30 1518 30 489 30 7267
Fragments arranged by size:
7267 1518 489 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3068 3176 3252 3613 3908 4202 4579 5497
Size: 1882 108 76 361 295 294 377 918
Cuts at: 5497 6300 1186
Size: 803 4445
Fragments arranged by size:
4445 1882 918 803 377 361 295 294
108 76
BseYI C'CCAG_C
Cuts at: 2800 3580 3691 3838 4889 5489 6123 6231 6802
Size: 780 111 147 1051 600 634 108 571
Cuts at: 6802 8762 9258 2800
Size: 1960 496 3101
Fragments arranged by size:
3101 1960 1051 780 634 600 571 496
147 111 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2441 5042 5723 1163
Size: 573 705 2601 681 4999
Fragments arranged by size:
4999 2601 705 681 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3225 4726 5499 7009 7770 8798
Size: 275 186 1224 1501 773 1510 761 1028
Cuts at: 8798 1540
Size: 2301
Fragments arranged by size:
2301 1510 1501 1224 1028 773 761 275
186
BsiHKAI G_wGCw'C
Cuts at: 2196 2942 3036 4147 4686 4709 4761 4785 5361
Size: 746 94 1111 539 23 52 24 576
Cuts at: 5361 5574 5778 7620 7705 8776 2196
Size: 213 204 1842 85 1071 2979
Fragments arranged by size:
2979 1842 1111 1071 746 576 539 213
204 94 85 52 24 23
BsmI GAATG_Cn'
Cuts at: 7070 7163 7070
Size: 93 9466
BsmBI CGTCTCn'nnnn_
Cuts at: 6001 6022 6833 6838 6001
Size: 21 811 5 8722
Fragments arranged by size:
8722 811 21 5
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2942 3036 4147 4175 4194 4574
Size: 399 406 746 94 1111 28 19 380
Cuts at: 4574 4686 4709 4761 4785 5353 5361 5574 5688
Size: 112 23 52 24 568 8 213 114
Cuts at: 5688 5778 6112 6642 6651 6878 7620 7705 8776
Size: 90 334 530 9 227 742 85 1071
Cuts at: 8776 1391
Size: 2174
Fragments arranged by size:
2174 1111 1071 746 742 568 530 406
399 380 334 227 213 114 112 94
90 85 52 28 24 23 19 9
8
BspEI T'CCGG_A
Cuts at: 3555 6042 9222 3555
Size: 2487 3180 3892
Fragments arranged by size:
3892 3180 2487
BspHI T'CATG_A
Cuts at: 7448 9178 7448
Size: 1730 7829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3644 5745 6579 6830 6914 844
Size: 2800 2101 834 251 84 3489
Fragments arranged by size:
3489 2800 2101 834 251 84
BsrBI CCG'CTC
Cuts at: 1394 1823 4728 5518 7446 1394
Size: 429 2905 790 1928 3507
Fragments arranged by size:
3507 2905 1928 790 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5484 8054 8228 65
Size: 1183 4236 2570 174 1396
Fragments arranged by size:
4236 2570 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2955 3220 4283 4803 5017 6127 6260
Size: 110 1145 265 1063 520 214 1110 133
Cuts at: 6260 6417 8203 8390 1700
Size: 157 1786 187 2869
Fragments arranged by size:
2869 1786 1145 1110 1063 520 265 214
187 157 133 110
BsrGI T'GTAC_A
Cuts at: 96 6579 96
Size: 6483 3076
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9559
BssSI C'ACGA_G
Cuts at: 2052 2780 3472 4694 7619 8631 2052
Size: 728 692 1222 2925 1012 2980
Fragments arranged by size:
2980 2925 1222 1012 728 692
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9559
BstBI TT'CG_AA
Cuts at: 3676 8363 3676
Size: 4687 4872
BstEII G'GTnAC_C
Cuts at: 1184 5095 1184
Size: 3911 5648
BstXI CCAn_nnnn'nTGG
Cuts at: 3049 3157 4126 6139 3049
Size: 108 969 2013 6469
Fragments arranged by size:
6469 2013 969 108
BstYI r'GATC_y
Cuts at: 1972 3450 5171 5556 5745 6588 6889 7256 7641
Size: 1478 1721 385 189 843 301 367 385
Cuts at: 7641 7658 9110 9196 9208 9554 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1721 1478 1452 843 385 385 367
346 301 189 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2255 2444 3143 3198 3261 3444
Size: 552 939 251 189 699 55 63 183
Cuts at: 3444 3629 5626 5838 6597 513
Size: 185 1997 212 759 3475
Fragments arranged by size:
3475 1997 939 759 699 552 251 212
189 185 183 63 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2622 5908 487
Size: 683 56 1396 3286 4138
Fragments arranged by size:
4138 3286 1396 683 56
BtsI GCAGTG_nn'
Cuts at: 3110 4678 5652 7075 7869 7889 3110
Size: 1568 974 1423 794 20 4780
Fragments arranged by size:
4780 1568 1423 974 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9559
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9524
DraI TTT'AAA
Cuts at: 4903 6863 4903
Size: 1960 7599
DraIII CAC_nnn'GTG
Cuts at: 3547 5301 6613 3547
Size: 1754 1312 6493
Fragments arranged by size:
6493 1754 1312
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5141 5891 8566 809
Size: 507 3825 750 2675 1802
Fragments arranged by size:
3825 2675 1802 750 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3222 3708 5496
Size: 54 1425 50 275 18 1392 486 1788
Cuts at: 5496 6129 6415 6594 7006 7895 9524 8
Size: 633 286 179 412 889 1629 43
Fragments arranged by size:
1788 1629 1425 1392 889 633 486 412
286 275 179 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3222 5496 7006 1537
Size: 275 1410 2274 1510 4090
Fragments arranged by size:
4090 2274 1510 1410 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2764 3165 3872 5466 6095 6671 7489 1353
Size: 1411 401 707 1594 629 576 818 3423
Fragments arranged by size:
3423 1594 1411 818 707 629 576 401
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5891 6677 8146 8520 8666 387
Size: 1064 138 4302 786 1469 374 146 1280
Fragments arranged by size:
4302 1469 1280 1064 786 374 146 138
EcoICRI GAG'CTC
Cuts at: 4707 4759 4783 5572 5776 4707
Size: 52 24 789 204 8490
Fragments arranged by size:
8490 789 204 52 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2210 2266 2367 2495 2748 2926
Size: 393 482 96 56 101 128 253 178
Cuts at: 2926 3179 3302 3641 4419 4896 5624 6176 6287
Size: 253 123 339 778 477 728 552 111
Cuts at: 6287 6368 7622 8219 9006 9274 1239
Size: 81 1254 597 787 268 1524
Fragments arranged by size:
1524 1254 787 778 728 597 552 482
477 393 339 268 253 253 178 128
123 111 101 96 81 56
EcoO109I rG'GnC_Cy
Cuts at: 2170 2659 3835 4394 5124 2170
Size: 489 1176 559 730 6605
Fragments arranged by size:
6605 1176 730 559 489
EcoRI G'AATT_C
Cuts at: 4051 6868 7246 4051
Size: 2817 378 6364
Fragments arranged by size:
6364 2817 378
EcoRV GAT'ATC
Cuts at: 3553 5056 3553
Size: 1503 8056
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3194 3194
Size: 9559
FspI TGC'GCA
Cuts at: 8065 8065
Size: 9559
HaeII r_GCGC'y
Cuts at: 1678 2398 3077 4552 4933 5726 6856 8706 1678
Size: 720 679 1475 381 793 1130 1850 2531
Fragments arranged by size:
2531 1850 1475 1130 793 720 679 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2231 2263 2624 2656 3196
Size: 32 135 32 659 32 361 32 540
Cuts at: 3196 3228 3365 3397 3440 3472 4064 4096 4622
Size: 32 137 32 43 32 592 32 526
Cuts at: 4622 4654 4856 4888 4934 4959 4966 4991 5358
Size: 32 202 32 46 25 7 25 367
Cuts at: 5358 5390 5561 5593 6279 6311 6463 6495 8200
Size: 32 171 32 686 32 152 32 1705
Cuts at: 8200 8232 8274 8306 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1705 686 659 592 540 526 367
361 202 171 152 137 135 46 43
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2224 3595 3787 3862 4465 6799
Size: 988 281 286 1371 192 75 603 2334
Cuts at: 6799 6903 7149 669
Size: 104 246 3079
Fragments arranged by size:
3079 2334 1371 988 603 286 281 246
192 104 75
HindIII A'AGCT_T
Cuts at: 748 3302 3524 3776 4013 748
Size: 2554 222 252 237 6294
Fragments arranged by size:
6294 2554 252 237 222
HpaI GTT'AAC
Cuts at: 7149 7149
Size: 9559
KpnI G_GTAC'C
Cuts at: 6884 6884
Size: 9559
MfeI C'AATT_G
Cuts at: 2180 7158 2180
Size: 4978 4581
MluI A'CGCG_T
Cuts at: 9435 9435
Size: 9559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3483 4228 4773 6285 8673 8857 1305
Size: 2178 745 545 1512 2388 184 2007
Fragments arranged by size:
2388 2178 2007 1512 745 545 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3710 6596 10
Size: 54 1425 343 1878 2886 2973
Fragments arranged by size:
2973 2886 1878 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2316 2780 3047 3437 4124 4509 5282
Size: 648 1150 464 267 390 687 385 773
Cuts at: 5282 5837 7517 7876 8035 518
Size: 555 1680 359 159 2042
Fragments arranged by size:
2042 1680 1150 773 687 648 555 464
390 385 359 267 159
MspA1I CmG'CkG
Cuts at: 1606 2257 3661 3691 3740 3842 3954 3992 5644
Size: 651 1404 30 49 102 112 38 1652
Cuts at: 5644 6115 6187 6231 6975 7652 8800 9045 1606
Size: 471 72 44 744 677 1148 245 2120
Fragments arranged by size:
2120 1652 1404 1148 744 677 651 471
245 112 102 72 49 44 38 30
NaeI GCC'GGC
Cuts at: 2957 3222 4805 6129 6419 2957
Size: 265 1583 1324 290 6097
Fragments arranged by size:
6097 1583 1324 290 265
NcoI C'CATG_G
Cuts at: 513 1065 2004 3143 3261 3444 5838 6597 513
Size: 552 939 1139 118 183 2394 759 3475
Fragments arranged by size:
3475 2394 1139 939 759 552 183 118
NdeI CA'TA_TG
Cuts at: 387 5853 387
Size: 5466 4093
NgoMIV G'CCGG_C
Cuts at: 2955 3220 4803 6127 6417 2955
Size: 265 1583 1324 290 6097
Fragments arranged by size:
6097 1583 1324 290 265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9559
NotI GC'GGCC_GC
Cuts at: 7006 7006
Size: 9559
NsiI A_TGCA'T
Cuts at: 5142 5142
Size: 9559
NspI r_CATG'y
Cuts at: 1132 1229 4138 5140 5144 6427 6636 6917 8462
Size: 97 2909 1002 4 1283 209 281 1545
Cuts at: 8462 1132
Size: 2229
Fragments arranged by size:
2909 2229 1545 1283 1002 281 209 97
4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9559
PciI A'CATG_T
Cuts at: 6423 6632 8458 6423
Size: 209 1826 7524
Fragments arranged by size:
7524 1826 209
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3262 3547 3940 4210 5075 6613 1108
Size: 2154 285 393 270 865 1538 4054
Fragments arranged by size:
4054 2154 1538 865 393 285 270
PfoI T'CCnGG_A
Cuts at: 4396 4643 4773 5559 5798 4396
Size: 247 130 786 239 8157
Fragments arranged by size:
8157 786 247 239 130
PmeI GTTT'AAAC
Cuts at: 6863 6863
Size: 9559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 7628 7660 9141 9173 3896
Size: 32 3700 32 1481 32 4282
Fragments arranged by size:
4282 3700 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2170 2659 4394 5124 2170
Size: 489 1735 730 6605
Fragments arranged by size:
6605 1735 730 489
PshAI GACnn'nnGTC
Cuts at: 1613 2971 1613
Size: 1358 8201
PsiI TTA'TAA
Cuts at: 7129 7129
Size: 9559
PstI C_TGCA'G
Cuts at: 830 3069 3117 4791 5466 6911 830
Size: 2239 48 1674 675 1445 3478
Fragments arranged by size:
3478 2239 1674 1445 675 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9559
PvuII CAG'CTG
Cuts at: 3661 3691 3740 3842 3992 5644 6115 6231 3661
Size: 30 49 102 150 1652 471 116 6989
Fragments arranged by size:
6989 1652 471 150 116 102 49 30
SacI G_AGCT'C
Cuts at: 4709 4761 4785 5574 5778 4709
Size: 52 24 789 204 8490
Fragments arranged by size:
8490 789 204 52 24
SacII CC_GC'GG
Cuts at: 2258 2258
Size: 9559
SalI G'TCGA_C
Cuts at: 1936 6901 1936
Size: 4965 4594
SapI GCTCTTCn'nnn_
Cuts at: 2764 3872 2764
Size: 1108 8451
SbfI CC_TGCA'GG
Cuts at: 5466 6911 5466
Size: 1445 8114
ScaI AGT'ACT
Cuts at: 5786 7807 9519 5786
Size: 2021 1712 5826
Fragments arranged by size:
5826 2021 1712
SexAI A'CCwGG_T
Cuts at: 4634 6609 4634
Size: 1975 7584
SfcI C'TryA_G
Cuts at: 826 1045 3065 3113 4787 5462 6907 8042 8723
Size: 219 2020 48 1674 675 1445 1135 681
Cuts at: 8723 8914 826
Size: 191 1471
Fragments arranged by size:
2020 1674 1471 1445 1135 681 675 219
191 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 6261 6261
Size: 9559
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9559
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9559
SmaI CCC'GGG
Cuts at: 1872 4249 4764 6886 1872
Size: 2377 515 2122 4545
Fragments arranged by size:
4545 2377 2122 515
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2356 3179 4573 5402 6078 7663
Size: 197 926 413 823 1394 829 676 1585
Cuts at: 7663 8564 8826 820
Size: 901 262 1553
Fragments arranged by size:
1585 1553 1394 926 901 829 823 676
413 262 197
SnaBI TAC'GTA
Cuts at: 493 7350 493
Size: 6857 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9559
SphI G_CATG'C
Cuts at: 1132 6917 1132
Size: 5785 3774
SspI AAT'ATT
Cuts at: 5 52 2124 4097 4998 5062 6316 7483 5
Size: 47 2072 1973 901 64 1254 1167 2081
Fragments arranged by size:
2081 2072 1973 1254 1167 901 64 47
StuI AGG'CCT
Cuts at: 1517 4425 5330 5931 1517
Size: 2908 905 601 5145
Fragments arranged by size:
5145 2908 905 601
StyI C'CwwG_G
Cuts at: 513 1065 2004 3143 3261 3369 3444 4470 5007
Size: 552 939 1139 118 108 75 1026 537
Cuts at: 5007 5325 5838 6597 6998 513
Size: 318 513 759 401 3074
Fragments arranged by size:
3074 1139 1026 939 759 552 537 513
401 318 118 108 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3469 3663 3947 4118 4421 5312 5543
Size: 1255 1889 194 284 171 303 891 231
Cuts at: 5543 7586 7603 7756 7941 8386 325
Size: 2043 17 153 185 445 1498
Fragments arranged by size:
2043 1889 1498 1255 891 445 303 284
231 194 185 171 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5784 6579 7805
Size: 274 80 33 51 1443 3807 795 1226
Cuts at: 7805 9517 96
Size: 1712 138
Fragments arranged by size:
3807 1712 1443 1226 795 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2921 2953 5996 6028 9519 9551 2921
Size: 32 3043 32 3491 32 2929
Fragments arranged by size:
3491 3043 2929 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4379 4651 1316
Size: 3063 272 6224
Fragments arranged by size:
6224 3063 272
XbaI T'CTAG_A
Cuts at: 6895 6895
Size: 9559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3141 3941 4323 5836 3141
Size: 800 382 1513 6864
Fragments arranged by size:
6864 1513 800 382
XhoI C'TCGA_G
Cuts at: 1943 5402 1943
Size: 3459 6100
XmaI C'CCGG_G
Cuts at: 1870 4247 4762 6884 1870
Size: 2377 515 2122 4545
Fragments arranged by size:
4545 2377 2122 515
XmnI GAAnn'nnTTC
Cuts at: 4051 7688 4051
Size: 3637 5922
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6367 7367 276
Size: 53 83 186 936 4833 1000 2468
Fragments arranged by size:
4833 2468 1000 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I
Bsu36I EcoNI FseI FspAI KasI NarI NruI PacI
PmlI PspOMI PspXI PsrI RsrII SanDI SfoI SrfI
SwaI