Restriction map for
pFN21AA0551
(Circular) (Six-base) MAPSORT of: pFN21AA0551.seq Check: 3270 from: 1 to: 8785
pFN21AA0551 8785 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2442 2442
Size: 8785
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4730 4958 6595 278
Size: 53 83 186 936 3194 228 1637 2468
Fragments arranged by size:
3194 2468 1637 936 228 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3047 4378 4947 5437 6128 1937
Size: 1110 1331 569 490 691 4594
Fragments arranged by size:
4594 1331 1110 691 569 490
Acc65I G'GTAC_C
Cuts at: 6106 6106
Size: 8785
AclI AA'CG_TT
Cuts at: 1563 6912 7285 1563
Size: 5349 373 3063
Fragments arranged by size:
5349 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2783 3497 3700 6848 8232 8500 1632
Size: 1151 714 203 3148 1384 268 1917
Fragments arranged by size:
3148 1917 1384 1151 714 268 203
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8588
AflIII A'CryG_T
Cuts at: 1203 2217 2373 7684 8661 1203
Size: 1014 156 5311 977 1327
Fragments arranged by size:
5311 1327 1014 977 156
AgeI A'CCGG_T
Cuts at: 7616 7616
Size: 8785
AhdI GACnn_n'nnGTC
Cuts at: 2653 5297 7514 2653
Size: 2644 2217 3924
Fragments arranged by size:
3924 2644 2217
AleI CACnn'nnGTG
Cuts at: 1166 5858 1166
Size: 4692 4093
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2419 1184
Size: 1235 7550
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3875 3907 3875
Size: 32 8753
AlwNI CAG_nnn'CTG
Cuts at: 3121 3166 3400 4571 6528 8100 3121
Size: 45 234 1171 1957 1572 3806
Fragments arranged by size:
3806 1957 1572 1171 234 45
ApaLI G'TGCA_C
Cuts at: 3836 6842 7998 3836
Size: 3006 1156 4623
Fragments arranged by size:
4623 3006 1156
ApoI r'AATT_y
Cuts at: 1493 2786 4063 5471 5537 6067 6094 6320 6472
Size: 1293 1277 1408 66 530 27 226 152
Cuts at: 6472 8581 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1408 1293 1277 530 226 152
66 27
AseI AT'TA_AT
Cuts at: 160 1032 7339 160
Size: 872 6307 1606
Fragments arranged by size:
6307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8785
AvaI C'yCGr_G
Cuts at: 1870 1943 6110 1870
Size: 73 4167 4545
Fragments arranged by size:
4545 4167 73
BamHI G'GATC_C
Cuts at: 4262 6115 6482 4262
Size: 1853 367 6565
Fragments arranged by size:
6565 1853 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2590 4018 6106 7561 618
Size: 325 217 627 803 1428 2088 1455 1842
Fragments arranged by size:
2088 1842 1455 1428 803 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2728 3018 4152 4166 4609 5521 5968 6104
Size: 1337 290 1134 14 443 912 447 136
Cuts at: 6104 1391
Size: 4072
Fragments arranged by size:
4072 1337 1134 912 447 443 290 136
14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4008 928
Size: 971 2109 5705
Fragments arranged by size:
5705 2109 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6976 7010 1903
Size: 34 5039 34 3678
Fragments arranged by size:
5039 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6677 7887 6677
Size: 1210 7575
BclI T'GATC_A
Cuts at: 1548 2352 5250 1548
Size: 804 2898 5083
Fragments arranged by size:
5083 2898 804
BfrBI ATG'CAT
Cuts at: 5956 5956
Size: 8785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7396 136
Size: 107 122 71 631 605 5724 1525
Fragments arranged by size:
5724 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2044 3381 5563 8780 2044
Size: 1337 2182 3217 2049
Fragments arranged by size:
3217 2182 2049 1337
BlpI GC'TnA_GC
Cuts at: 2532 6202 2532
Size: 3670 5115
Bme1580I G_kGCm'C
Cuts at: 1790 3840 6846 8002 1790
Size: 2050 3006 1156 2573
Fragments arranged by size:
3006 2573 2050 1156
BmgBI CAC'GTC
Cuts at: 1318 5856 1318
Size: 4538 4247
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3823 4368 4853
Size: 416 53 83 399 24 2406 545 485
Cuts at: 4853 6091 6516 7474 442
Size: 1238 425 958 1753
Fragments arranged by size:
2406 1753 1238 958 545 485 425 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4389 1056
Size: 3333 5452
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3022 3037 4833 4894 3022
Size: 15 1796 61 6913
Fragments arranged by size:
6913 1796 61 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3143 3239 4180 4186 4483 4593 4679 4752
Size: 1904 96 941 6 297 110 86 73
Cuts at: 4752 4803 5552 6048 7445 1239
Size: 51 749 496 1397 2579
Fragments arranged by size:
2579 1904 1397 941 749 496 297 110
96 86 73 51 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2894 6910 7775 8073 2894
Size: 4016 865 298 3606
Fragments arranged by size:
4016 3606 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2868 4544 4655 7448 882
Size: 617 1369 1676 111 2793 2219
Fragments arranged by size:
2793 2219 1676 1369 617 111
BsaAI yAC'GTr
Cuts at: 493 1204 6576 493
Size: 711 5372 2702
Fragments arranged by size:
5372 2702 711
BsaBI GATnn'nnATC
Cuts at: 4410 8779 4410
Size: 4369 4416
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4727 4955 6592 6974
Size: 53 83 186 936 3194 228 1637 382
Cuts at: 6974 7609 275
Size: 635 1451
Fragments arranged by size:
3194 1637 1451 936 635 382 228 186
83 53
BsaWI w'CCGG_w
Cuts at: 2241 4137 4446 5256 5916 7218 7616 7890 8037
Size: 1896 309 810 660 1302 398 274 147
Cuts at: 8037 8448 2241
Size: 411 2578
Fragments arranged by size:
2578 1896 1302 810 660 411 398 309
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3875 3905 4481 4511 4591
Size: 30 165 30 2472 30 576 30 80
Cuts at: 4591 4621 1178
Size: 30 5342
Fragments arranged by size:
5342 2472 576 165 80 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 2958 3008 3011 3161 3206 3236 3302
Size: 1213 559 50 3 150 45 30 66
Cuts at: 3302 3320 3323 3328 3374 3377 3936 4483 4486
Size: 18 3 5 46 3 559 547 3
Cuts at: 4486 4520 4544 5712 1186
Size: 34 24 1168 4259
Fragments arranged by size:
4259 1213 1168 559 559 547 150 66
50 46 45 34 30 24 18 5
3 3 3 3
BseYI C'CCAG_C
Cuts at: 3017 4045 4218 4223 4242 5892 7988 8484 3017
Size: 1028 173 5 19 1650 2096 496 3318
Fragments arranged by size:
3318 2096 1650 1028 496 173 19 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 4823 5181 1163
Size: 573 684 2403 358 4767
Fragments arranged by size:
4767 2403 684 573 358
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6235 6996 8024 1540
Size: 275 186 4234 761 1028 2301
Fragments arranged by size:
4234 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2533 2695 2728 3153 3840 4087 4609 5835 5968
Size: 162 33 425 687 247 522 1226 133
Cuts at: 5968 6846 6931 8002 2533
Size: 878 85 1071 3316
Fragments arranged by size:
3316 1226 1071 878 687 522 425 247
162 133 85 33
BsmI GAATG_Cn'
Cuts at: 2901 5500 6296 6389 2901
Size: 2599 796 93 5297
Fragments arranged by size:
5297 2599 796 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4718 4858 4962 4718
Size: 140 104 8541
Fragments arranged by size:
8541 140 104
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2533 2695 2728 3018 3153 3840 4087
Size: 399 743 162 33 290 135 687 247
Cuts at: 4087 4152 4166 4609 5521 5835 5968 6104 6846
Size: 65 14 443 912 314 133 136 742
Cuts at: 6846 6931 8002 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 912 743 742 687 443 399
314 290 247 162 136 135 133 85
65 33 14
BspEI T'CCGG_A
Cuts at: 4137 4446 8448 4137
Size: 309 4002 4474
Fragments arranged by size:
4474 4002 309
BspHI T'CATG_A
Cuts at: 6051 6674 8404 6051
Size: 623 1730 6432
Fragments arranged by size:
6432 1730 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2442 3702 3820 6140 844
Size: 1598 1260 118 2320 3489
Fragments arranged by size:
3489 2320 1598 1260 118
BsrBI CCG'CTC
Cuts at: 1394 1823 6672 1394
Size: 429 4849 3507
Fragments arranged by size:
4849 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4771 7280 7454 65
Size: 1183 3523 2509 174 1396
Fragments arranged by size:
3523 2509 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3276 3636 7429 7616 1700
Size: 110 1466 360 3793 187 2869
Fragments arranged by size:
3793 2869 1466 360 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8785
BssSI C'ACGA_G
Cuts at: 3839 6845 7857 3839
Size: 3006 1012 4767
Fragments arranged by size:
4767 3006 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2690 3400 794
Size: 1896 710 6179
Fragments arranged by size:
6179 1896 710
BstBI TT'CG_AA
Cuts at: 7589 7589
Size: 8785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8785
BstXI CCAn_nnnn'nTGG
Cuts at: 2680 5860 2680
Size: 3180 5605
BstYI r'GATC_y
Cuts at: 1972 2044 2073 3381 3777 3925 4103 4262 4411
Size: 72 29 1308 396 148 178 159 149
Cuts at: 4411 5563 5913 6115 6482 6867 6884 8336 8422
Size: 1152 350 202 367 385 17 1452 86
Cuts at: 8422 8434 8780 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1308 1152 396 385 367 350
346 202 178 159 149 148 86 72
29 17 12
BstZ17I GTA'TAC
Cuts at: 4948 5438 4948
Size: 490 8295
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7294
Fragments arranged by size:
7294 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8046
Fragments arranged by size:
8046 683 56
BtsI GCAGTG_nn'
Cuts at: 4189 6301 7095 7115 4189
Size: 2112 794 20 5859
Fragments arranged by size:
5859 2112 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3943 3978 529
Size: 35 3379 35 5336
Fragments arranged by size:
5336 3379 35 35
DraI TTT'AAA
Cuts at: 6089 6089
Size: 8785
DraIII CAC_nnn'GTG
Cuts at: 3901 3901
Size: 8785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2522 7792 809
Size: 507 1206 5270 1802
Fragments arranged by size:
5270 1802 1206 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3081 3168 4750
Size: 54 1425 50 275 18 1251 87 1582
Cuts at: 4750 4777 5809 6232 7121 8750 8
Size: 27 1032 423 889 1629 43
Fragments arranged by size:
1629 1582 1425 1251 1032 889 423 275
87 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 6232 1537
Size: 275 4420 4090
Fragments arranged by size:
4420 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 2735 2985 4521 4971 6715 1353
Size: 831 551 250 1536 450 1744 3423
Fragments arranged by size:
3423 1744 1536 831 551 450 250
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7372 7746 7892 387
Size: 1064 138 5783 374 146 1280
Fragments arranged by size:
5783 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2726 4607 5966 2726
Size: 1881 1359 5545
Fragments arranged by size:
5545 1881 1359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2783 3143 3239 3497 3700 4180 4186
Size: 393 1151 360 96 258 203 480 6
Cuts at: 4186 4483 4593 4679 4752 4803 5552 6048 6848
Size: 297 110 86 73 51 749 496 800
Cuts at: 6848 7445 8232 8500 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1151 800 787 749 597 496 480
393 360 297 268 258 203 110 96
86 73 51 6
EcoNI CCTnn'n_nnAGG
Cuts at: 4171 4171
Size: 8785
EcoO109I rG'GnC_Cy
Cuts at: 2062 3103 3809 3856 4091 2062
Size: 1041 706 47 235 6756
Fragments arranged by size:
6756 1041 706 235 47
EcoRI G'AATT_C
Cuts at: 4063 6094 6472 4063
Size: 2031 378 6376
Fragments arranged by size:
6376 2031 378
EcoRV GAT'ATC
Cuts at: 3681 5686 3681
Size: 2005 6780
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2665 3434 4050 2665
Size: 769 616 7400
Fragments arranged by size:
7400 769 616
FspI TGC'GCA
Cuts at: 7291 7291
Size: 8785
HaeII r_GCGC'y
Cuts at: 1678 2756 3277 7932 1678
Size: 1078 521 4655 2531
Fragments arranged by size:
4655 2531 1078 521
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2639 2671 2845 2877 3769
Size: 32 135 32 1067 32 174 32 892
Cuts at: 3769 3801 3917 3949 3965 3997 4478 4510 4973
Size: 32 116 32 16 32 481 32 463
Cuts at: 4973 4976 5005 5008 5283 5315 5903 5935 7426
Size: 3 29 3 275 32 588 32 1491
Cuts at: 7426 7458 7500 7532 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1491 1067 892 588 481 463 275
174 135 116 42 32 32 32 32
32 32 32 32 32 32 32 32
29 16 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 4379 6129 6375 669
Size: 988 281 1110 1331 1750 246 3079
Fragments arranged by size:
3079 1750 1331 1110 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8785
HpaI GTT'AAC
Cuts at: 6375 6375
Size: 8785
KpnI G_GTAC'C
Cuts at: 6110 6110
Size: 8785
MfeI C'AATT_G
Cuts at: 2849 3440 6384 2849
Size: 591 2944 5250
Fragments arranged by size:
5250 2944 591
MluI A'CGCG_T
Cuts at: 8661 8661
Size: 8785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2077 3687 7899 8083 1305
Size: 772 1610 4212 184 2007
Fragments arranged by size:
4212 2007 1610 772 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3083 4752 4779 10
Size: 54 1425 343 1251 1669 27 4016
Fragments arranged by size:
4016 1669 1425 1251 343 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2678 5858 5940 6743 7102 7261 518
Size: 648 1512 3180 82 803 359 159 2042
Fragments arranged by size:
3180 2042 1512 803 648 359 159 82
MspA1I CmG'CkG
Cuts at: 1606 3079 3121 3136 3163 3523 4686 4823 6201
Size: 1473 42 15 27 360 1163 137 1378
Cuts at: 6201 6878 8026 8271 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1473 1378 1163 1148 677 360 245
137 42 27 15
NaeI GCC'GGC
Cuts at: 3278 3278
Size: 8785
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7294
Fragments arranged by size:
7294 939 552
NdeI CA'TA_TG
Cuts at: 387 2632 387
Size: 2245 6540
NgoMIV G'CCGG_C
Cuts at: 3276 3276
Size: 8785
NheI G'CTAG_C
Cuts at: 1052 4385 1052
Size: 3333 5452
NotI GC'GGCC_GC
Cuts at: 6232 6232
Size: 8785
NsiI A_TGCA'T
Cuts at: 5958 5958
Size: 8785
NspI r_CATG'y
Cuts at: 1132 1229 2221 2713 6143 7688 1132
Size: 97 992 492 3430 1545 2229
Fragments arranged by size:
3430 2229 1545 992 492 97
PasI CC'CwG_GG
Cuts at: 1348 3805 1348
Size: 2457 6328
PciI A'CATG_T
Cuts at: 2217 7684 2217
Size: 5467 3318
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3901 4873 1108
Size: 2793 972 5020
Fragments arranged by size:
5020 2793 972
PmeI GTTT'AAAC
Cuts at: 6089 6089
Size: 8785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 6854 6886 8367 8399 3875
Size: 32 2947 32 1481 32 4261
Fragments arranged by size:
4261 2947 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2062 3809 4091 2062
Size: 1747 282 6756
Fragments arranged by size:
6756 1747 282
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8785
PsiI TTA'TAA
Cuts at: 2124 5445 6355 2124
Size: 3321 910 4554
Fragments arranged by size:
4554 3321 910
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3399 3431 3399
Size: 32 8753
PstI C_TGCA'G
Cuts at: 830 2071 2408 3078 3474 6137 830
Size: 1241 337 670 396 2663 3478
Fragments arranged by size:
3478 2663 1241 670 396 337
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8785
PvuII CAG'CTG
Cuts at: 3079 3121 3163 4823 3079
Size: 42 42 1660 7041
Fragments arranged by size:
7041 1660 42 42
SacI G_AGCT'C
Cuts at: 2728 4609 5968 2728
Size: 1881 1359 5545
Fragments arranged by size:
5545 1881 1359
SalI G'TCGA_C
Cuts at: 1936 3046 4377 6127 1936
Size: 1110 1331 1750 4594
Fragments arranged by size:
4594 1750 1331 1110
SanDI GG'GwC_CC
Cuts at: 2062 4091 2062
Size: 2029 6756
SbfI CC_TGCA'GG
Cuts at: 2071 6137 2071
Size: 4066 4719
ScaI AGT'ACT
Cuts at: 7033 8745 7033
Size: 1712 7073
SexAI A'CCwGG_T
Cuts at: 4797 4797
Size: 8785
SfcI C'TryA_G
Cuts at: 826 1045 2067 2404 3074 3470 4401 6133 7268
Size: 219 1022 337 670 396 931 1732 1135
Cuts at: 7268 7949 8140 826
Size: 681 191 1471
Fragments arranged by size:
1732 1471 1135 1022 931 681 670 396
337 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8785
SmaI CCC'GGG
Cuts at: 1872 6112 1872
Size: 4240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2909 6889 7790 8052 820
Size: 197 926 966 3980 901 262 1553
Fragments arranged by size:
3980 1553 966 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6576 493
Size: 6083 2702
SpeI A'CTAG_T
Cuts at: 152 2509 152
Size: 2357 6428
SphI G_CATG'C
Cuts at: 1132 6143 1132
Size: 5011 3774
SspI AAT'ATT
Cuts at: 5 52 2231 2248 6709 5
Size: 47 2179 17 4461 2081
Fragments arranged by size:
4461 2179 2081 47 17
StuI AGG'CCT
Cuts at: 1517 3538 1517
Size: 2021 6764
StyI C'CwwG_G
Cuts at: 513 1065 2004 3606 3701 4231 4335 6224 513
Size: 552 939 1602 95 530 104 1889 3074
Fragments arranged by size:
3074 1889 1602 939 552 530 104 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3990 5235 6812 6829 6982 7167 7612
Size: 1255 2410 1245 1577 17 153 185 445
Cuts at: 7612 325
Size: 1498
Fragments arranged by size:
2410 1577 1498 1255 1245 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2970 4626 7031
Size: 274 80 33 51 1443 993 1656 2405
Cuts at: 7031 8743 96
Size: 1712 138
Fragments arranged by size:
2405 1712 1656 1443 993 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4066 4098 4168 4200 4299 4331 8745 8777 4066
Size: 32 70 32 99 32 4414 32 4074
Fragments arranged by size:
4414 4074 99 70 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8785
XbaI T'CTAG_A
Cuts at: 6121 6121
Size: 8785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 4820 5884 2167
Size: 2653 1064 5068
Fragments arranged by size:
5068 2653 1064
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8785
XmaI C'CCGG_G
Cuts at: 1870 6110 1870
Size: 4240 4545
XmnI GAAnn'nnTTC
Cuts at: 2564 4005 4033 4063 5062 5138 6914 2564
Size: 1441 28 30 999 76 1776 4435
Fragments arranged by size:
4435 1776 1441 999 76 30 28
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4728 4956 6593 276
Size: 53 83 186 936 3194 228 1637 2468
Fragments arranged by size:
3194 2468 1637 936 228 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI Bsu36I FseI FspAI KasI NarI NruI PacI
PfoI PmlI PspOMI PspXI RsrII SacII SapI SfiI
SfoI SrfI SwaI