Restriction map for
pFN21AA0541
(Circular) (Six-base) MAPSORT of: pFN21AA0541.seq Check: 6024 from: 1 to: 9175
pFN21AA0541 9175 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6985 278
Size: 53 83 186 936 5449 2468
Fragments arranged by size:
5449 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6518 1937
Size: 4581 4594
Acc65I G'GTAC_C
Cuts at: 6496 6496
Size: 9175
AclI AA'CG_TT
Cuts at: 1563 7302 7675 1563
Size: 5739 373 3063
Fragments arranged by size:
5739 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2582 4137 5274 7238 8622 8890 1632
Size: 950 1555 1137 1964 1384 268 1917
Fragments arranged by size:
1964 1917 1555 1384 1137 950 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8978
AflIII A'CryG_T
Cuts at: 1203 3407 4069 6279 8074 9051 1203
Size: 2204 662 2210 1795 977 1327
Fragments arranged by size:
2210 2204 1795 1327 977 662
AgeI A'CCGG_T
Cuts at: 8006 8006
Size: 9175
AhdI GACnn_n'nnGTC
Cuts at: 7904 7904
Size: 9175
AleI CACnn'nnGTG
Cuts at: 1166 6200 1166
Size: 5034 4141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6320 1184
Size: 5136 4039
AlwNI CAG_nnn'CTG
Cuts at: 4619 5092 5252 5750 6918 8490 4619
Size: 473 160 498 1168 1572 5304
Fragments arranged by size:
5304 1572 1168 498 473 160
ApaLI G'TGCA_C
Cuts at: 7232 8388 7232
Size: 1156 8019
ApoI r'AATT_y
Cuts at: 1493 2312 2920 3997 4159 5012 5340 5411 5469
Size: 819 608 1077 162 853 328 71 58
Cuts at: 5469 5919 5937 6484 6710 6862 8971 1493
Size: 450 18 547 226 152 2109 1697
Fragments arranged by size:
2109 1697 1077 853 819 608 547 450
328 226 162 152 71 58 18
AseI AT'TA_AT
Cuts at: 160 1032 2166 7729 160
Size: 872 1134 5563 1606
Fragments arranged by size:
5563 1606 1134 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9175
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 6500 1870
Size: 73 229 4328 4545
Fragments arranged by size:
4545 4328 229 73
BamHI G'GATC_C
Cuts at: 5501 6505 6872 5501
Size: 1004 367 7804
Fragments arranged by size:
7804 1004 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6316 6349 6496 7951 618
Size: 325 217 627 4529 33 147 1455 1842
Fragments arranged by size:
4529 1842 1455 627 325 217 147 33
BanII G_rGCy'C
Cuts at: 1391 4199 5158 5744 6121 6494 1391
Size: 2808 959 586 377 373 4072
Fragments arranged by size:
4072 2808 959 586 377 373
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 6032 928
Size: 971 4133 4071
Fragments arranged by size:
4133 4071 971
BbvCI CC'TCA_GC
Cuts at: 6321 6321
Size: 9175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5672 5706 5736 5770 7366 7400 1903
Size: 34 3735 34 30 34 1596 34 3678
Fragments arranged by size:
3735 3678 1596 34 34 34 34 30
BciVI GTATCCnnnnn_n'
Cuts at: 2596 3766 7067 8277 2596
Size: 1170 3301 1210 3494
Fragments arranged by size:
3494 3301 1210 1170
BclI T'GATC_A
Cuts at: 1548 3445 4738 4888 1548
Size: 1897 1293 150 5835
Fragments arranged by size:
5835 1897 1293 150
BfrBI ATG'CAT
Cuts at: 3660 3660
Size: 9175
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5087 7786 136
Size: 107 122 71 631 605 3415 2699 1525
Fragments arranged by size:
3415 2699 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9170 9170
Size: 9175
BlpI GC'TnA_GC
Cuts at: 6592 6592
Size: 9175
Bme1580I G_kGCm'C
Cuts at: 1790 6354 7236 8392 1790
Size: 4564 882 1156 2573
Fragments arranged by size:
4564 2573 1156 882
BmgBI CAC'GTC
Cuts at: 1318 5185 5624 6282 1318
Size: 3867 439 658 4211
Fragments arranged by size:
4211 3867 658 439
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5009 6906 7864
Size: 416 53 83 399 24 3592 1897 958
Cuts at: 7864 442
Size: 1753
Fragments arranged by size:
3592 1897 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3486 3528 5051 5217 7835 1239
Size: 2247 42 1523 166 2618 2579
Fragments arranged by size:
2618 2579 2247 1523 166 42
Bpu10I CC'TnA_GC
Cuts at: 6321 6321
Size: 9175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6377 7300 8165 8463 6377
Size: 923 865 298 7089
Fragments arranged by size:
7089 923 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3436 7838 882
Size: 617 1937 4402 2219
Fragments arranged by size:
4402 2219 1937 617
BsaAI yAC'GTr
Cuts at: 493 1204 6966 493
Size: 711 5762 2702
Fragments arranged by size:
5762 2702 711
BsaBI GATnn'nnATC
Cuts at: 4887 9169 4887
Size: 4282 4893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6982 7364 7999 275
Size: 53 83 186 936 5449 382 635 1451
Fragments arranged by size:
5449 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6150 7608 8006 8280 8427 8838 6150
Size: 1458 398 274 147 411 6487
Fragments arranged by size:
6487 1458 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4659 4689 5218 5248 1178
Size: 30 165 30 3256 30 529 30 5105
Fragments arranged by size:
5105 3256 529 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2636 4307 4316 4539 1186
Size: 1450 1671 9 223 5822
Fragments arranged by size:
5822 1671 1450 223 9
BseYI C'CCAG_C
Cuts at: 5743 6055 6289 8378 8874 5743
Size: 312 234 2089 496 6044
Fragments arranged by size:
6044 2089 496 312 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4480 4842 6380 1163
Size: 573 2744 362 1538 3958
Fragments arranged by size:
3958 2744 1538 573 362
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2106 6625 7386 8414 1540
Size: 275 186 105 4519 761 1028 2301
Fragments arranged by size:
4519 2301 1028 761 275 186 105
BsiHKAI G_wGCw'C
Cuts at: 2179 6121 6462 7236 7321 8392 2179
Size: 3942 341 774 85 1071 2962
Fragments arranged by size:
3942 2962 1071 774 341 85
BsmI GAATG_Cn'
Cuts at: 4485 6686 6779 4485
Size: 2201 93 6881
Fragments arranged by size:
6881 2201 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3898 3898
Size: 9175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2179 4199 5158 5744 6121 6354 6462
Size: 399 389 2020 959 586 377 233 108
Cuts at: 6462 6494 7236 7321 8392 1391
Size: 32 742 85 1071 2174
Fragments arranged by size:
2174 2020 1071 959 742 586 399 389
377 233 108 85 32
BspEI T'CCGG_A
Cuts at: 6150 8838 6150
Size: 2688 6487
BspHI T'CATG_A
Cuts at: 7064 8794 7064
Size: 1730 7445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2869 3313 6057 6530 844
Size: 2025 444 2744 473 3489
Fragments arranged by size:
3489 2744 2025 473 444
BsrBI CCG'CTC
Cuts at: 1394 1823 4874 5763 6094 7062 1394
Size: 429 3051 889 331 968 3507
Fragments arranged by size:
3507 3051 968 889 429 331
BsrDI GCAATG_nn'
Cuts at: 65 1248 2062 3126 5787 7670 7844 65
Size: 1183 814 1064 2661 1883 174 1396
Fragments arranged by size:
2661 1883 1396 1183 1064 814 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7819 8006 1700
Size: 110 6009 187 2869
Fragments arranged by size:
6009 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2935 3243 96
Size: 2839 308 6028
Fragments arranged by size:
6028 2839 308
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9175
BssSI C'ACGA_G
Cuts at: 5904 5978 7235 8247 5904
Size: 74 1257 1012 6832
Fragments arranged by size:
6832 1257 1012 74
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5884 794
Size: 5090 4085
BstBI TT'CG_AA
Cuts at: 7979 7979
Size: 9175
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9175
BstYI r'GATC_y
Cuts at: 1972 2143 5501 5971 6017 6505 6872 7257 7274
Size: 171 3358 470 46 488 367 385 17
Cuts at: 7274 8726 8812 8824 9170 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
3358 1977 1452 488 470 385 367 346
171 86 46 17 12
Bsu36I CC'TnA_GG
Cuts at: 5245 5475 5245
Size: 230 8945
BtgI C'CryG_G
Cuts at: 513 1065 2004 2398 3385 5229 6124 6175 513
Size: 552 939 394 987 1844 895 51 3513
Fragments arranged by size:
3513 1844 987 939 895 552 394 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8436
Fragments arranged by size:
8436 683 56
BtsI GCAGTG_nn'
Cuts at: 2902 3595 4398 4492 6195 6207 6284 6691 7485
Size: 693 803 94 1703 12 77 407 794
Cuts at: 7485 7505 2902
Size: 20 4572
Fragments arranged by size:
4572 1703 803 794 693 407 94 77
20 12
ClaI AT'CG_AT
Cuts at: 1554 5059 1554
Size: 3505 5670
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5947 5982 529
Size: 35 5383 35 3722
Fragments arranged by size:
5383 3722 35 35
DraI TTT'AAA
Cuts at: 6479 6479
Size: 9175
DraIII CAC_nnn'GTG
Cuts at: 3386 3386
Size: 9175
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2133 8182 809
Size: 507 817 6049 1802
Fragments arranged by size:
6049 1802 817 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3318 3365 5517
Size: 54 1425 50 275 18 1488 47 2152
Cuts at: 5517 6622 7511 9140 8
Size: 1105 889 1629 43
Fragments arranged by size:
2152 1629 1488 1425 1105 889 275 54
50 47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6622 1537
Size: 275 4810 4090
Fragments arranged by size:
4810 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3352 4235 4284 5268 7105 1353
Size: 1999 883 49 984 1837 3423
Fragments arranged by size:
3423 1999 1837 984 883 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5117 7762 8136 8282 387
Size: 1064 138 3528 2645 374 146 1280
Fragments arranged by size:
3528 2645 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 6119 6119
Size: 9175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2582 3486 3528 4137 5051 5217 5274
Size: 393 950 904 42 609 914 166 57
Cuts at: 5274 7238 7835 8622 8890 1239
Size: 1964 597 787 268 1524
Fragments arranged by size:
1964 1524 950 914 904 787 609 597
393 268 166 57 42
EcoNI CCTnn'n_nnAGG
Cuts at: 4793 4793
Size: 9175
EcoO109I rG'GnC_Cy
Cuts at: 2759 4754 5089 5249 2759
Size: 1995 335 160 6685
Fragments arranged by size:
6685 1995 335 160
EcoRI G'AATT_C
Cuts at: 6484 6862 6484
Size: 378 8797
EcoRV GAT'ATC
Cuts at: 2472 3462 6227 2472
Size: 990 2765 5420
Fragments arranged by size:
5420 2765 990
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2405 2682 2405
Size: 277 8898
FspI TGC'GCA
Cuts at: 7681 7681
Size: 9175
HaeII r_GCGC'y
Cuts at: 1678 2057 8322 1678
Size: 379 6265 2531
Fragments arranged by size:
6265 2531 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2612 2644 2786 2818 3895
Size: 32 135 32 1040 32 142 32 1077
Cuts at: 3895 3927 5437 5469 7816 7848 7890 7922 1373
Size: 32 1510 32 2347 32 42 32 2626
Fragments arranged by size:
2626 2347 1510 1077 1040 142 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3883 5290 5490 6519 6765 669
Size: 988 281 1945 1407 200 1029 246 3079
Fragments arranged by size:
3079 1945 1407 1029 988 281 246 200
HindIII A'AGCT_T
Cuts at: 748 2220 748
Size: 1472 7703
HpaI GTT'AAC
Cuts at: 6765 6765
Size: 9175
KpnI G_GTAC'C
Cuts at: 6500 6500
Size: 9175
MfeI C'AATT_G
Cuts at: 6774 6774
Size: 9175
MluI A'CGCG_T
Cuts at: 9051 9051
Size: 9175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4258 4838 4934 5529 6094 6444 8289 8473
Size: 2953 580 96 595 565 350 1845 184
Cuts at: 8473 1305
Size: 2007
Fragments arranged by size:
2953 2007 1845 595 580 565 350 184
96
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3367 5519 10
Size: 54 1425 343 1535 2152 3666
Fragments arranged by size:
3666 2152 1535 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3540 4070 5654 5723 5999 6200 6302
Size: 648 2374 530 1584 69 276 201 102
Cuts at: 6302 6437 7133 7492 7651 518
Size: 135 696 359 159 2042
Fragments arranged by size:
2374 2042 1584 696 648 530 359 276
201 159 135 102 69
MspA1I CmG'CkG
Cuts at: 1606 2075 3089 3193 3331 5663 5750 6325 6591
Size: 469 1014 104 138 2332 87 575 266
Cuts at: 6591 7268 8416 8661 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2332 2120 1148 1014 677 575 469 266
245 138 104 87
NcoI C'CATG_G
Cuts at: 513 1065 2004 6124 6175 513
Size: 552 939 4120 51 3513
Fragments arranged by size:
4120 3513 939 552 51
NdeI CA'TA_TG
Cuts at: 387 4533 4743 387
Size: 4146 210 4819
Fragments arranged by size:
4819 4146 210
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9175
NotI GC'GGCC_GC
Cuts at: 6622 6622
Size: 9175
NsiI A_TGCA'T
Cuts at: 3662 3662
Size: 9175
NspI r_CATG'y
Cuts at: 1132 1229 2599 3352 3411 4073 5777 6307 6533
Size: 97 1370 753 59 662 1704 530 226
Cuts at: 6533 8078 1132
Size: 1545 2229
Fragments arranged by size:
2229 1704 1545 1370 753 662 530 226
97 59
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9175
PciI A'CATG_T
Cuts at: 3407 4069 8074 3407
Size: 662 4005 4508
Fragments arranged by size:
4508 4005 662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2715 3257 1108
Size: 1607 542 7026
Fragments arranged by size:
7026 1607 542
PfoI T'CCnGG_A
Cuts at: 5265 5265
Size: 9175
PmeI GTTT'AAAC
Cuts at: 6479 6479
Size: 9175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3336 3368 4209 4241 7244 7276 8757 8789 3336
Size: 32 841 32 3003 32 1481 32 3722
Fragments arranged by size:
3722 3003 1481 841 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2759 4754 2759
Size: 1995 7180
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9175
PsiI TTA'TAA
Cuts at: 4557 6745 4557
Size: 2188 6987
PspXI vC'TCGA_Gb
Cuts at: 2172 2172
Size: 9175
PstI C_TGCA'G
Cuts at: 830 6068 6202 6527 830
Size: 5238 134 325 3478
Fragments arranged by size:
5238 3478 325 134
PvuI CG_AT'CG
Cuts at: 2001 2106 2001
Size: 105 9070
PvuII CAG'CTG
Cuts at: 3089 3193 3331 5750 6325 3089
Size: 104 138 2419 575 5939
Fragments arranged by size:
5939 2419 575 138 104
SacI G_AGCT'C
Cuts at: 6121 6121
Size: 9175
SalI G'TCGA_C
Cuts at: 1936 6517 1936
Size: 4581 4594
SapI GCTCTTCn'nnn_
Cuts at: 5268 5268
Size: 9175
SbfI CC_TGCA'GG
Cuts at: 6527 6527
Size: 9175
ScaI AGT'ACT
Cuts at: 4449 7423 9135 4449
Size: 2974 1712 4489
Fragments arranged by size:
4489 2974 1712
SexAI A'CCwGG_T
Cuts at: 3726 6345 3726
Size: 2619 6556
SfcI C'TryA_G
Cuts at: 826 1045 3577 3609 5555 6064 6198 6523 7658
Size: 219 2532 32 1946 509 134 325 1135
Cuts at: 7658 8339 8530 826
Size: 681 191 1471
Fragments arranged by size:
2532 1946 1471 1135 681 509 325 219
191 134 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9175
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9175
SmaI CCC'GGG
Cuts at: 1872 6502 1872
Size: 4630 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2172 6392 7279 8180 8442 820
Size: 197 926 229 4220 887 901 262 1553
Fragments arranged by size:
4220 1553 926 901 887 262 229 197
SnaBI TAC'GTA
Cuts at: 493 6966 493
Size: 6473 2702
SpeI A'CTAG_T
Cuts at: 152 5492 152
Size: 5340 3835
SphI G_CATG'C
Cuts at: 1132 5777 6307 6533 1132
Size: 4645 530 226 3774
Fragments arranged by size:
4645 3774 530 226
SspI AAT'ATT
Cuts at: 5 52 2616 2714 3020 3067 4010 4828 7099
Size: 47 2564 98 306 47 943 818 2271
Cuts at: 7099 5
Size: 2081
Fragments arranged by size:
2564 2271 2081 943 818 306 98 47
47
StuI AGG'CCT
Cuts at: 1517 3712 5020 1517
Size: 2195 1308 5672
Fragments arranged by size:
5672 2195 1308
StyI C'CwwG_G
Cuts at: 513 1065 2004 4474 6124 6175 6614 513
Size: 552 939 2470 1650 51 439 3074
Fragments arranged by size:
3074 2470 1650 939 552 439 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4335 4866 7202 7219 7372 7557 8002
Size: 1255 2755 531 2336 17 153 185 445
Cuts at: 8002 325
Size: 1498
Fragments arranged by size:
2755 2336 1498 1255 531 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2935 3243 3325
Size: 274 80 33 51 1443 958 308 82
Cuts at: 3325 3589 4447 5172 6003 7421 9133 96
Size: 264 858 725 831 1418 1712 138
Fragments arranged by size:
1712 1443 1418 958 858 831 725 308
274 264 138 82 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4659 4691 5218 5250 9135 9167 4659
Size: 32 527 32 3885 32 4667
Fragments arranged by size:
4667 3885 527 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2798 3909 5183 1316
Size: 1482 1111 1274 5308
Fragments arranged by size:
5308 1482 1274 1111
XbaI T'CTAG_A
Cuts at: 6511 6511
Size: 9175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2363 5512 5722 5965 2363
Size: 3149 210 243 5573
Fragments arranged by size:
5573 3149 243 210
XhoI C'TCGA_G
Cuts at: 1943 2172 1943
Size: 229 8946
XmaI C'CCGG_G
Cuts at: 1870 6500 1870
Size: 4630 4545
XmnI GAAnn'nnTTC
Cuts at: 3843 7304 3843
Size: 3461 5714
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6983 276
Size: 53 83 186 936 5449 2468
Fragments arranged by size:
5449 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BstXI BstZ17I FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspOMI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI