Restriction map for
pFN21AA0528
(Circular) (Six-base) MAPSORT of: pFN21AA0528.seq Check: 4799 from: 1 to: 7705
pFN21AA0528 7705 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5515 278
Size: 53 83 186 936 3979 2468
Fragments arranged by size:
3979 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2388 2908 5048 1937
Size: 451 520 2140 4594
Fragments arranged by size:
4594 2140 520 451
Acc65I G'GTAC_C
Cuts at: 5026 5026
Size: 7705
AclI AA'CG_TT
Cuts at: 1563 5832 6205 1563
Size: 4269 373 3063
Fragments arranged by size:
4269 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 3936 4092 4167 4365 4788 5007 5768
Size: 407 1897 156 75 198 423 219 761
Cuts at: 5768 7152 7420 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1897 1384 761 423 407 268 219
198 156 75
AflII C'TTAA_G
Cuts at: 820 1017 2135 820
Size: 197 1118 6390
Fragments arranged by size:
6390 1118 197
AflIII A'CryG_T
Cuts at: 1203 3091 3383 4692 6604 7581 1203
Size: 1888 292 1309 1912 977 1327
Fragments arranged by size:
1912 1888 1327 1309 977 292
AgeI A'CCGG_T
Cuts at: 6536 6536
Size: 7705
AhdI GACnn_n'nnGTC
Cuts at: 6434 6434
Size: 7705
AleI CACnn'nnGTG
Cuts at: 1166 3752 1166
Size: 2586 5119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7705
AlwNI CAG_nnn'CTG
Cuts at: 3305 5448 7020 3305
Size: 2143 1572 3990
Fragments arranged by size:
3990 2143 1572
ApaI G_GGCC'C
Cuts at: 2994 2994
Size: 7705
ApaLI G'TGCA_C
Cuts at: 3111 5762 6918 3111
Size: 2651 1156 3898
Fragments arranged by size:
3898 2651 1156
ApoI r'AATT_y
Cuts at: 1493 2099 2174 2438 2547 3044 3373 3699 4261
Size: 606 75 264 109 497 329 326 562
Cuts at: 4261 4324 4576 4609 5014 5240 5392 7501 1493
Size: 63 252 33 405 226 152 2109 1697
Fragments arranged by size:
2109 1697 606 562 497 405 329 326
264 252 226 152 109 75 63 33
AseI AT'TA_AT
Cuts at: 160 1032 4355 6259 160
Size: 872 3323 1904 1606
Fragments arranged by size:
3323 1904 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7705
AvaI C'yCGr_G
Cuts at: 1870 1943 3165 5030 1870
Size: 73 1222 1865 4545
Fragments arranged by size:
4545 1865 1222 73
AvrII C'CTAG_G
Cuts at: 3947 3947
Size: 7705
BamHI G'GATC_C
Cuts at: 5035 5402 5035
Size: 367 7338
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2040 3335 5026 6481 618
Size: 325 217 627 253 1295 1691 1455 1842
Fragments arranged by size:
1842 1691 1455 1295 627 325 253 217
BanII G_rGCy'C
Cuts at: 1391 2994 4792 5024 1391
Size: 1603 1798 232 4072
Fragments arranged by size:
4072 1798 1603 232
BbeI G_GCGC'C
Cuts at: 2044 2044
Size: 7705
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2199 2526 3809 4840 928
Size: 971 300 327 1283 1031 3793
Fragments arranged by size:
3793 1283 1031 971 327 300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5896 5930 1903
Size: 34 50 34 3875 34 3678
Fragments arranged by size:
3875 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2366 3149 5597 6807 2366
Size: 783 2448 1210 3264
Fragments arranged by size:
3264 2448 1210 783
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7705
BfrBI ATG'CAT
Cuts at: 3173 3173
Size: 7705
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6316 136
Size: 107 122 71 631 605 4644 1525
Fragments arranged by size:
4644 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3971 7700 3971
Size: 3729 3976
BlpI GC'TnA_GC
Cuts at: 3736 5122 3736
Size: 1386 6319
Bme1580I G_kGCm'C
Cuts at: 1790 2994 3115 5766 6922 1790
Size: 1204 121 2651 1156 2573
Fragments arranged by size:
2651 2573 1204 1156 121
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7705
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5436 6394 442
Size: 416 53 83 399 24 4019 958 1753
Fragments arranged by size:
4019 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7705
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2708 3888 4677 6365 1239
Size: 1469 1180 789 1688 2579
Fragments arranged by size:
2579 1688 1469 1180 789
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3943 4253 5830 6695 6993 3943
Size: 310 1577 865 298 4655
Fragments arranged by size:
4655 1577 865 310 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6368 882
Size: 617 4869 2219
Fragments arranged by size:
4869 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5496 493
Size: 711 4292 2702
Fragments arranged by size:
4292 2702 711
BsaBI GATnn'nnATC
Cuts at: 2283 2406 2826 3976 7699 2283
Size: 123 420 1150 3723 2289
Fragments arranged by size:
3723 2289 1150 420 123
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2041 5512 5894 6529
Size: 53 83 186 936 508 3471 382 635
Cuts at: 6529 275
Size: 1451
Fragments arranged by size:
3471 1451 936 635 508 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4581 6138 6536 6810 6957 7368 4581
Size: 1557 398 274 147 411 4918
Fragments arranged by size:
4918 1557 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2681 2711 1178
Size: 30 165 30 1278 30 6172
Fragments arranged by size:
6172 1278 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4092 4749 1186
Size: 2906 657 4142
Fragments arranged by size:
4142 2906 657
BseYI C'CCAG_C
Cuts at: 2755 3797 6908 7404 2755
Size: 1042 3111 496 3056
Fragments arranged by size:
3111 3056 1042 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7132
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5155 5916 6944 1540
Size: 275 186 3154 761 1028 2301
Fragments arranged by size:
3154 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3115 4792 5766 5851 6922 3115
Size: 1677 974 85 1071 3898
Fragments arranged by size:
3898 1677 1071 974 85
BsmI GAATG_Cn'
Cuts at: 2762 3685 4819 5216 5309 2762
Size: 923 1134 397 93 5158
Fragments arranged by size:
5158 1134 923 397 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2994 3115 4792 5024 5766 5851 6922
Size: 399 1204 121 1677 232 742 85 1071
Cuts at: 6922 1391
Size: 2174
Fragments arranged by size:
2174 1677 1204 1071 742 399 232 121
85
BspEI T'CCGG_A
Cuts at: 4581 7368 4581
Size: 2787 4918
BspHI T'CATG_A
Cuts at: 5594 7324 5594
Size: 1730 5975
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5060 844
Size: 4216 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5592 1394
Size: 429 3769 3507
Fragments arranged by size:
3769 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4439 4785 6200 6374 65
Size: 1183 3191 346 1415 174 1396
Fragments arranged by size:
3191 1415 1396 1183 346 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6349 6536 1700
Size: 110 4539 187 2869
Fragments arranged by size:
4539 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2271 3088 96
Size: 2175 817 4713
Fragments arranged by size:
4713 2175 817
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7705
BssSI C'ACGA_G
Cuts at: 5765 6777 5765
Size: 1012 6693
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2255 794
Size: 1461 6244
BstBI TT'CG_AA
Cuts at: 6509 6509
Size: 7705
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7705
BstYI r'GATC_y
Cuts at: 1972 2821 3971 5035 5402 5787 5804 7256 7342
Size: 849 1150 1064 367 385 17 1452 86
Cuts at: 7342 7354 7700 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1150 1064 849 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2359 3339 4065 513
Size: 552 1294 980 726 4153
Fragments arranged by size:
4153 1294 980 726 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6966
Fragments arranged by size:
6966 683 56
BtsI GCAGTG_nn'
Cuts at: 4847 4914 5221 6015 6035 4847
Size: 67 307 794 20 6517
Fragments arranged by size:
6517 794 307 67 20
ClaI AT'CG_AT
Cuts at: 1554 2410 1554
Size: 856 6849
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4545 4580 529
Size: 35 3981 35 3654
Fragments arranged by size:
3981 3654 35 35
DraI TTT'AAA
Cuts at: 2119 2173 2406 2998 4285 5009 2119
Size: 54 233 592 1287 724 4815
Fragments arranged by size:
4815 1287 724 592 233 54
DraIII CAC_nnn'GTG
Cuts at: 3389 4604 3389
Size: 1215 6490
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6712 809
Size: 507 5396 1802
Fragments arranged by size:
5396 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 5152 6041
Size: 54 1425 50 275 18 204 3118 889
Cuts at: 6041 7670 8
Size: 1629 43
Fragments arranged by size:
3118 1629 1425 889 275 204 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5152 1537
Size: 275 3340 4090
Fragments arranged by size:
4090 3340 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2399 2923 3264 3912 4725 5635 1353
Size: 1046 524 341 648 813 910 3423
Fragments arranged by size:
3423 1046 910 813 648 524 341
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6292 6666 6812 387
Size: 1064 138 4703 374 146 1280
Fragments arranged by size:
4703 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4790 4790
Size: 7705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2708 3888 3936 4092 4167 4365
Size: 393 407 669 1180 48 156 75 198
Cuts at: 4365 4677 4788 5007 5768 6365 7152 7420 1239
Size: 312 111 219 761 597 787 268 1524
Fragments arranged by size:
1524 1180 787 761 669 597 407 393
312 268 219 198 156 111 75 48
EcoNI CCTnn'n_nnAGG
Cuts at: 4107 4107
Size: 7705
EcoO109I rG'GnC_Cy
Cuts at: 2849 2990 2991 2849
Size: 141 1 7563
Fragments arranged by size:
7563 141 1
EcoRI G'AATT_C
Cuts at: 2547 3699 5014 5392 2547
Size: 1152 1315 378 4860
Fragments arranged by size:
4860 1315 1152 378
EcoRV GAT'ATC
Cuts at: 3912 3912
Size: 7705
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2644 3557 2644
Size: 913 6792
FspI TGC'GCA
Cuts at: 6211 6211
Size: 7705
HaeII r_GCGC'y
Cuts at: 1678 2044 6852 1678
Size: 366 4808 2531
Fragments arranged by size:
4808 2531 366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2571 2603 2681 2713 3921
Size: 32 135 32 999 32 78 32 1208
Cuts at: 3921 3953 4284 4316 4624 4656 4702 4734 6346
Size: 32 331 32 308 32 46 32 1612
Cuts at: 6346 6378 6420 6452 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1612 1208 999 331 308 135 78
46 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2909 5049 5295 669
Size: 988 281 971 2140 246 3079
Fragments arranged by size:
3079 2140 988 971 281 246
HindIII A'AGCT_T
Cuts at: 748 3125 748
Size: 2377 5328
HpaI GTT'AAC
Cuts at: 5295 5295
Size: 7705
KasI G'GCGC_C
Cuts at: 2040 2040
Size: 7705
KpnI G_GTAC'C
Cuts at: 5030 5030
Size: 7705
MfeI C'AATT_G
Cuts at: 3867 5304 3867
Size: 1437 6268
MluI A'CGCG_T
Cuts at: 7581 7581
Size: 7705
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3012 6819 7003 1305
Size: 1707 3807 184 2007
Fragments arranged by size:
3807 2007 1707 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5883
Fragments arranged by size:
5883 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3752 5663 6022 6181 518
Size: 648 2586 1911 359 159 2042
Fragments arranged by size:
2586 2042 1911 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3302 5121 5798 6946 7191 1606
Size: 1696 1819 677 1148 245 2120
Fragments arranged by size:
2120 1819 1696 1148 677 245
NarI GG'CG_CC
Cuts at: 2041 2041
Size: 7705
NcoI C'CATG_G
Cuts at: 513 1065 4065 513
Size: 552 3000 4153
Fragments arranged by size:
4153 3000 552
NdeI CA'TA_TG
Cuts at: 387 3409 3823 3843 387
Size: 3022 414 20 4249
Fragments arranged by size:
4249 3022 414 20
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7705
NotI GC'GGCC_GC
Cuts at: 5152 5152
Size: 7705
NsiI A_TGCA'T
Cuts at: 3175 3175
Size: 7705
NspI r_CATG'y
Cuts at: 1132 1229 2778 3212 4696 5063 6608 1132
Size: 97 1549 434 1484 367 1545 2229
Fragments arranged by size:
2229 1549 1545 1484 434 367 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7705
PciI A'CATG_T
Cuts at: 4692 6604 4692
Size: 1912 5793
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3779 1108
Size: 2671 5034
PfoI T'CCnGG_A
Cuts at: 3076 4654 3076
Size: 1578 6127
PmeI GTTT'AAAC
Cuts at: 5009 5009
Size: 7705
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5774 5806 7287 7319 5774
Size: 32 1481 32 6160
Fragments arranged by size:
6160 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2849 2849
Size: 7705
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7705
PsiI TTA'TAA
Cuts at: 4889 5275 4889
Size: 386 7319
PspOMI G'GGCC_C
Cuts at: 2990 2990
Size: 7705
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2716 2748 2716
Size: 32 7673
PstI C_TGCA'G
Cuts at: 830 2616 3330 3464 5057 830
Size: 1786 714 134 1593 3478
Fragments arranged by size:
3478 1786 1593 714 134
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7705
SacI G_AGCT'C
Cuts at: 4792 4792
Size: 7705
SalI G'TCGA_C
Cuts at: 1936 2907 5047 1936
Size: 971 2140 4594
Fragments arranged by size:
4594 2140 971
SbfI CC_TGCA'GG
Cuts at: 5057 5057
Size: 7705
ScaI AGT'ACT
Cuts at: 2637 5953 7665 2637
Size: 3316 1712 2677
Fragments arranged by size:
3316 2677 1712
SfcI C'TryA_G
Cuts at: 826 1045 2470 2612 2902 3234 3244 3326 3460
Size: 219 1425 142 290 332 10 82 134
Cuts at: 3460 3498 3597 5053 6188 6869 7060 826
Size: 38 99 1456 1135 681 191 1471
Fragments arranged by size:
1471 1456 1425 1135 681 332 290 219
191 142 134 99 82 38 10
SfoI GGC'GCC
Cuts at: 2042 2042
Size: 7705
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7705
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7705
SmaI CCC'GGG
Cuts at: 1872 5032 1872
Size: 3160 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2135 3165 3958 4268 5809 6710
Size: 197 926 192 1030 793 310 1541 901
Cuts at: 6710 6972 820
Size: 262 1553
Fragments arranged by size:
1553 1541 1030 926 901 793 310 262
197 192
SnaBI TAC'GTA
Cuts at: 493 5496 493
Size: 5003 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7705
SphI G_CATG'C
Cuts at: 1132 5063 1132
Size: 3931 3774
SspI AAT'ATT
Cuts at: 5 52 5629 5
Size: 47 5577 2081
Fragments arranged by size:
5577 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7705
StyI C'CwwG_G
Cuts at: 513 1065 2558 3947 4065 5144 513
Size: 552 1493 1389 118 1079 3074
Fragments arranged by size:
3074 1493 1389 1079 552 118
SwaI ATTT'AAAT
Cuts at: 2406 2406
Size: 7705
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2962 3704 3949 4556 5732 5749 5902
Size: 1255 1382 742 245 607 1176 17 153
Cuts at: 5902 6087 6532 325
Size: 185 445 1498
Fragments arranged by size:
1498 1382 1255 1176 742 607 445 245
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 2635 2732
Size: 274 80 33 51 1443 294 364 97
Cuts at: 2732 3088 3307 4278 4982 5951 7663 96
Size: 356 219 971 704 969 1712 138
Fragments arranged by size:
1712 1443 971 969 704 364 356 294
274 219 138 97 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7665 7697 7665
Size: 32 7673
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7705
XbaI T'CTAG_A
Cuts at: 5041 5041
Size: 7705
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2532 2956 2532
Size: 424 7281
XhoI C'TCGA_G
Cuts at: 1943 3165 1943
Size: 1222 6483
XmaI C'CCGG_G
Cuts at: 1870 5030 1870
Size: 3160 4545
XmnI GAAnn'nnTTC
Cuts at: 4356 5834 4356
Size: 1478 6227
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5513 276
Size: 53 83 186 936 3979 2468
Fragments arranged by size:
3979 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I FseI FspAI NaeI
NgoMIV NruI PacI PmlI PspXI PvuII RsrII SacII
SanDI SapI SexAI SfiI SrfI