Restriction map for
pFN21AA0523
(Circular) (Six-base) MAPSORT of: pFN21AA0523.seq Check: 1746 from: 1 to: 6430
pFN21AA0523 6430 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4240 278
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2556 2994 3773 1937
Size: 619 438 779 4594
Fragments arranged by size:
4594 779 619 438
Acc65I G'GTAC_C
Cuts at: 3751 3751
Size: 6430
AclI AA'CG_TT
Cuts at: 1563 4557 4930 1563
Size: 2994 373 3063
Fragments arranged by size:
3063 2994 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2434 4493 5877 6145 1632
Size: 802 2059 1384 268 1917
Fragments arranged by size:
2059 1917 1384 802 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 3096 3531 5329 6306 1203
Size: 1893 435 1798 977 1327
Fragments arranged by size:
1893 1798 1327 977 435
AgeI A'CCGG_T
Cuts at: 5261 5261
Size: 6430
AhdI GACnn_n'nnGTC
Cuts at: 5159 5159
Size: 6430
AleI CACnn'nnGTG
Cuts at: 1166 3240 1166
Size: 2074 4356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2117 2234 3512 1184
Size: 933 117 1278 4102
Fragments arranged by size:
4102 1278 933 117
AlwNI CAG_nnn'CTG
Cuts at: 2056 2880 2974 4173 5745 2056
Size: 824 94 1199 1572 2741
Fragments arranged by size:
2741 1572 1199 824 94
ApaI G_GGCC'C
Cuts at: 2141 2399 2141
Size: 258 6172
ApaLI G'TGCA_C
Cuts at: 3467 4487 5643 3467
Size: 1020 1156 4254
Fragments arranged by size:
4254 1156 1020
ApoI r'AATT_y
Cuts at: 1493 3317 3739 3965 4117 6226 1493
Size: 1824 422 226 152 2109 1697
Fragments arranged by size:
2109 1824 1697 422 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 2142 2229 2399 2833 3755 1870
Size: 73 199 87 170 434 922 4545
Fragments arranged by size:
4545 922 434 199 170 87 73
BamHI G'GATC_C
Cuts at: 3760 4127 3760
Size: 367 6063
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2146 2432 3108 3339 3496
Size: 325 217 627 359 286 676 231 157
Cuts at: 3496 3751 5206 618
Size: 255 1455 1842
Fragments arranged by size:
1842 1455 676 627 359 325 286 255
231 217 157
BanII G_rGCy'C
Cuts at: 1391 2141 2218 2276 2399 2663 2723 3624 3749
Size: 750 77 58 123 264 60 901 125
Cuts at: 3749 1391
Size: 4072
Fragments arranged by size:
4072 901 750 264 125 123 77 60
58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5459
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2210 2244 2999 3033 4621 4655 1903
Size: 34 273 34 755 34 1588 34 3678
Fragments arranged by size:
3678 1588 755 273 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4322 5532 4322
Size: 1210 5220
BclI T'GATC_A
Cuts at: 1548 3648 1548
Size: 2100 4330
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2183 5041 136
Size: 107 122 71 631 605 511 2858 1525
Fragments arranged by size:
2858 1525 631 605 511 122 107 71
BglII A'GATC_T
Cuts at: 6425 6425
Size: 6430
BlpI GC'TnA_GC
Cuts at: 2541 3847 2541
Size: 1306 5124
Bme1580I G_kGCm'C
Cuts at: 1790 2141 2149 2399 3106 3342 3471 3501 3726
Size: 351 8 250 707 236 129 30 225
Cuts at: 3726 4491 5647 1790
Size: 765 1156 2573
Fragments arranged by size:
2573 1156 765 707 351 250 236 225
129 30 8
BmgBI CAC'GTC
Cuts at: 1318 3427 1318
Size: 2109 4321
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4161 5119 442
Size: 416 53 83 399 24 2744 958 1753
Fragments arranged by size:
2744 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2012 2707 5090 1239
Size: 773 695 2383 2579
Fragments arranged by size:
2579 2383 773 695
Bpu10I CC'TnA_GC
Cuts at: 3618 3618
Size: 6430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2521 3425 4555 5420 5718 2521
Size: 904 1130 865 298 3233
Fragments arranged by size:
3233 1130 904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5093 882
Size: 617 3594 2219
Fragments arranged by size:
3594 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3099 4221 493
Size: 711 1895 1122 2702
Fragments arranged by size:
2702 1895 1122 711
BsaBI GATnn'nnATC
Cuts at: 6424 6424
Size: 6430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4237 4619 5254 275
Size: 53 83 186 936 2704 382 635 1451
Fragments arranged by size:
2704 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2310 3288 3663 4863 5261 5535 5682 6093 2310
Size: 978 375 1200 398 274 147 411 2647
Fragments arranged by size:
2647 1200 978 411 398 375 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2672 2702 1178
Size: 30 165 30 1269 30 4906
Fragments arranged by size:
4906 1269 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3491 3560 1186
Size: 2305 69 4056
Fragments arranged by size:
4056 2305 69
BseYI C'CCAG_C
Cuts at: 2188 2251 2792 2880 5633 6129 2188
Size: 63 541 88 2753 496 2489
Fragments arranged by size:
2753 2489 541 496 88 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 1163
Size: 573 468 5389
Fragments arranged by size:
5389 573 468
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3601 3880 4641 5669 1540
Size: 275 186 1600 279 761 1028 2301
Fragments arranged by size:
2301 1600 1028 761 279 275 186
BsiHKAI G_wGCw'C
Cuts at: 3471 4491 4576 5647 3471
Size: 1020 85 1071 4254
Fragments arranged by size:
4254 1071 1020 85
BsmI GAATG_Cn'
Cuts at: 2891 3941 4034 2891
Size: 1050 93 5287
Fragments arranged by size:
5287 1050 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2149 2218 2276 2399 2663 2723
Size: 399 351 8 69 58 123 264 60
Cuts at: 2723 3106 3342 3471 3501 3624 3726 3749 4491
Size: 383 236 129 30 123 102 23 742
Cuts at: 4491 4576 5647 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 383 351 264 236
129 123 123 102 85 69 60 58
30 23 8
BspEI T'CCGG_A
Cuts at: 2310 3288 3663 6093 2310
Size: 978 375 2430 2647
Fragments arranged by size:
2647 2430 978 375
BspHI T'CATG_A
Cuts at: 2364 4319 6049 2364
Size: 1955 1730 2745
Fragments arranged by size:
2745 1955 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2928 3785 844
Size: 2084 857 3489
Fragments arranged by size:
3489 2084 857
BsrBI CCG'CTC
Cuts at: 1394 1823 2545 3550 4317 1394
Size: 429 722 1005 767 3507
Fragments arranged by size:
3507 1005 767 722 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4925 5099 65
Size: 1183 3677 174 1396
Fragments arranged by size:
3677 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2090 2561 2598 2682 3600 5074 5261
Size: 110 280 471 37 84 918 1474 187
Cuts at: 5261 1700
Size: 2869
Fragments arranged by size:
2869 1474 918 471 280 187 110 84
37
BsrGI T'GTAC_A
Cuts at: 96 3023 96
Size: 2927 3503
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6430
BssSI C'ACGA_G
Cuts at: 2465 3236 3409 4490 5502 2465
Size: 771 173 1081 1012 3393
Fragments arranged by size:
3393 1081 1012 771 173
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2167 794
Size: 1373 5057
BstBI TT'CG_AA
Cuts at: 5234 5234
Size: 6430
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6430
BstXI CCAn_nnnn'nTGG
Cuts at: 3242 3242
Size: 6430
BstYI r'GATC_y
Cuts at: 1972 3760 4127 4512 4529 5981 6067 6079 6425
Size: 1788 367 385 17 1452 86 12 346
Cuts at: 6425 1972
Size: 1977
Fragments arranged by size:
1977 1788 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2961 2961
Size: 6430
BtgI C'CryG_G
Cuts at: 513 1065 2004 2181 2749 2820 2931 513
Size: 552 939 177 568 71 111 4012
Fragments arranged by size:
4012 939 568 552 177 111 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5691
Fragments arranged by size:
5691 683 56
BtsI GCAGTG_nn'
Cuts at: 2521 3946 4740 4760 2521
Size: 1425 794 20 4191
Fragments arranged by size:
4191 1425 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6395
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2231 5437 809
Size: 507 915 3206 1802
Fragments arranged by size:
3206 1802 915 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2175 2461
Size: 54 1425 50 275 18 177 168 286
Cuts at: 2461 2865 2950 3383 3598 3877 4766 6395 8
Size: 404 85 433 215 279 889 1629 43
Fragments arranged by size:
1629 1425 889 433 404 286 279 275
215 177 168 85 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3598 3877 1537
Size: 275 1786 279 4090
Fragments arranged by size:
4090 1786 279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2622 3365 4360 1353
Size: 1269 743 995 3423
Fragments arranged by size:
3423 1269 995 743
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 2594 2749 3220 5017 5391
Size: 1064 138 441 564 155 471 1797 374
Cuts at: 5391 5537 387
Size: 146 1280
Fragments arranged by size:
1797 1280 1064 564 471 441 374 155
146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2012 2434 2707 4493 5090 5877 6145
Size: 393 380 422 273 1786 597 787 268
Cuts at: 6145 1239
Size: 1524
Fragments arranged by size:
1786 1524 787 597 422 393 380 273
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2804 2804
Size: 6430
EcoO109I rG'GnC_Cy
Cuts at: 2137 2138 2396 2956 3303 2137
Size: 1 258 560 347 5264
Fragments arranged by size:
5264 560 347 258 1
EcoRI G'AATT_C
Cuts at: 3317 3739 4117 3317
Size: 422 378 5630
Fragments arranged by size:
5630 422 378
FspI TGC'GCA
Cuts at: 4936 4936
Size: 6430
HaeII r_GCGC'y
Cuts at: 1678 2189 3607 5577 1678
Size: 511 1418 1970 2531
Fragments arranged by size:
2531 1970 1418 511
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2672 2704 3204 3236 5071
Size: 32 135 32 1100 32 500 32 1835
Cuts at: 5071 5103 5145 5177 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1835 1100 500 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3397 3774 4020 669
Size: 988 281 1459 377 246 3079
Fragments arranged by size:
3079 1459 988 377 281 246
HindIII A'AGCT_T
Cuts at: 748 3076 748
Size: 2328 4102
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KpnI G_GTAC'C
Cuts at: 3755 3755
Size: 6430
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 6306 6306
Size: 6430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2047 2785 5544 5728 1305
Size: 742 738 2759 184 2007
Fragments arranged by size:
2759 2007 742 738 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2463 2867 2952 10
Size: 54 1425 343 177 454 404 85 3488
Fragments arranged by size:
3488 1425 454 404 343 177 85 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3240 4388 4747 4906 518
Size: 648 2074 1148 359 159 2042
Fragments arranged by size:
2074 2042 1148 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2113 2304 2349 2609 2751 3356 3846 4523
Size: 507 191 45 260 142 605 490 677
Cuts at: 4523 5671 5916 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 605 507 490 260 245
191 142 45
NaeI GCC'GGC
Cuts at: 2563 3602 2563
Size: 1039 5391
NcoI C'CATG_G
Cuts at: 513 1065 2004 2931 513
Size: 552 939 927 4012
Fragments arranged by size:
4012 939 927 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6430
NgoMIV G'CCGG_C
Cuts at: 2561 3600 2561
Size: 1039 5391
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 3877 3877
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 2266 2786 3125 3788 5333 1132
Size: 97 1037 520 339 663 1545 2229
Fragments arranged by size:
2229 1545 1037 663 520 339 97
PasI CC'CwG_GG
Cuts at: 1348 2377 2392 3711 1348
Size: 1029 15 1319 4067
Fragments arranged by size:
4067 1319 1029 15
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6430
PfoI T'CCnGG_A
Cuts at: 2723 3429 2723
Size: 706 5724
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PmlI CAC'GTG
Cuts at: 3099 3099
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 6012 6044 4499
Size: 32 1481 32 4885
Fragments arranged by size:
4885 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2956 3303 2956
Size: 347 6083
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 4000 4000
Size: 6430
PspOMI G'GGCC_C
Cuts at: 2137 2395 2137
Size: 258 6172
PspXI vC'TCGA_Gb
Cuts at: 2229 2229
Size: 6430
PstI C_TGCA'G
Cuts at: 830 2112 2163 2271 2715 3782 830
Size: 1282 51 108 444 1067 3478
Fragments arranged by size:
3478 1282 1067 444 108 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
PvuII CAG'CTG
Cuts at: 3356 3356
Size: 6430
RsrII CG'GwC_CG
Cuts at: 2736 2736
Size: 6430
SacII CC_GC'GG
Cuts at: 2752 2752
Size: 6430
SalI G'TCGA_C
Cuts at: 1936 3772 1936
Size: 1836 4594
SapI GCTCTTCn'nnn_
Cuts at: 3365 3365
Size: 6430
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 4678 6390 4678
Size: 1712 4718
SfcI C'TryA_G
Cuts at: 826 1045 2108 2159 2267 2711 3778 4913 5594
Size: 219 1063 51 108 444 1067 1135 681
Cuts at: 5594 5785 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1067 1063 681 444 219 191
108 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6430
SmaI CCC'GGG
Cuts at: 1872 2144 3757 1872
Size: 272 1613 4545
Fragments arranged by size:
4545 1613 272
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2229 2500 3440 4534 5435 5697
Size: 197 926 286 271 940 1094 901 262
Cuts at: 5697 820
Size: 1553
Fragments arranged by size:
1553 1094 940 926 901 286 271 262
197
SnaBI TAC'GTA
Cuts at: 493 4221 493
Size: 3728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 3125 3788 1132
Size: 1993 663 3774
Fragments arranged by size:
3774 1993 663
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6430
StyI C'CwwG_G
Cuts at: 513 1065 2004 2862 2931 3869 513
Size: 552 939 858 69 938 3074
Fragments arranged by size:
3074 939 938 858 552 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2243 4457 4474 4627 4812 5257 325
Size: 1255 663 2214 17 153 185 445 1498
Fragments arranged by size:
2214 1498 1255 663 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3023 4676 6388
Size: 274 80 33 51 1443 1046 1653 1712
Cuts at: 6388 96
Size: 138
Fragments arranged by size:
1712 1653 1443 1046 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2772 2804 6390 6422 2772
Size: 32 3586 32 2780
Fragments arranged by size:
3586 2780 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3392 1316
Size: 2076 4354
XbaI T'CTAG_A
Cuts at: 3766 3766
Size: 6430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2818 3061 2818
Size: 243 6187
XhoI C'TCGA_G
Cuts at: 1943 2229 1943
Size: 286 6144
XmaI C'CCGG_G
Cuts at: 1870 2142 3755 1870
Size: 272 1613 4545
Fragments arranged by size:
4545 1613 272
XmnI GAAnn'nnTTC
Cuts at: 2855 4559 2855
Size: 1704 4726
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4238 276
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PsrI
SacI SanDI SexAI SfiI SfoI SrfI SwaI