Restriction map for
pFN21AA0522
(Circular) (Six-base) MAPSORT of: pFN21AA0522.seq Check: 2940 from: 1 to: 9169
pFN21AA0522 9169 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2487 2487
Size: 9169
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6979 278
Size: 53 83 186 936 5443 2468
Fragments arranged by size:
5443 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4915 6512 1937
Size: 2978 1597 4594
Fragments arranged by size:
4594 2978 1597
Acc65I G'GTAC_C
Cuts at: 6490 6490
Size: 9169
AclI AA'CG_TT
Cuts at: 1563 7296 7669 1563
Size: 5733 373 3063
Fragments arranged by size:
5733 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3526 3920 3978 4567 6157 7232 8616 8884
Size: 1894 394 58 589 1590 1075 1384 268
Cuts at: 8884 1632
Size: 1917
Fragments arranged by size:
1917 1894 1590 1384 1075 589 394 268
58
AfeI AGC'GCT
Cuts at: 2948 2948
Size: 9169
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8972
AflIII A'CryG_T
Cuts at: 1203 4455 4686 8068 9045 1203
Size: 3252 231 3382 977 1327
Fragments arranged by size:
3382 3252 1327 977 231
AgeI A'CCGG_T
Cuts at: 2438 4357 8000 2438
Size: 1919 3643 3607
Fragments arranged by size:
3643 3607 1919
AhdI GACnn_n'nnGTC
Cuts at: 4846 7898 4846
Size: 3052 6117
AleI CACnn'nnGTG
Cuts at: 1166 4358 1166
Size: 3192 5977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5450 1184
Size: 4266 4903
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2306 2338 3276 3308 2306
Size: 32 938 32 8167
Fragments arranged by size:
8167 938 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2110 2113 2278 3956 4327 5156 5803 6912 8484
Size: 3 165 1678 371 829 647 1109 1572
Cuts at: 8484 2110
Size: 2795
Fragments arranged by size:
2795 1678 1572 1109 829 647 371 165
3
ApaI G_GGCC'C
Cuts at: 2025 2855 3462 3928 3987 4020 5655 5834 5997
Size: 830 607 466 59 33 1635 179 163
Cuts at: 5997 6158 6221 6402 2025
Size: 161 63 181 4792
Fragments arranged by size:
4792 1635 830 607 466 181 179 163
161 63 59 33
ApaLI G'TGCA_C
Cuts at: 7226 8382 7226
Size: 1156 8013
ApoI r'AATT_y
Cuts at: 1493 2725 4418 5028 5113 6478 6704 6856 8965
Size: 1232 1693 610 85 1365 226 152 2109
Cuts at: 8965 1493
Size: 1697
Fragments arranged by size:
2109 1697 1693 1365 1232 610 226 152
85
AseI AT'TA_AT
Cuts at: 160 1032 7723 160
Size: 872 6691 1606
Fragments arranged by size:
6691 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9169
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 2035 2251 2337 2542 3632 3690
Size: 73 83 9 216 86 205 1090 58
Cuts at: 3690 3731 4155 4779 6494 1870
Size: 41 424 624 1715 4545
Fragments arranged by size:
4545 1715 1090 624 424 216 205 86
83 73 58 41 9
AvrII C'CTAG_G
Cuts at: 4423 4423
Size: 9169
BamHI G'GATC_C
Cuts at: 2039 6224 6499 6866 2039
Size: 4185 275 367 4342
Fragments arranged by size:
4342 4185 367 275
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2366 2651 3251 3263 3643
Size: 325 217 627 579 285 600 12 380
Cuts at: 3643 4945 6028 6490 7945 618
Size: 1302 1083 462 1455 1842
Fragments arranged by size:
1842 1455 1302 1083 627 600 579 462
380 325 285 217 12
BanII G_rGCy'C
Cuts at: 1391 2025 2051 2642 2855 2884 3035 3294 3462
Size: 634 26 591 213 29 151 259 168
Cuts at: 3462 3928 3987 4020 4109 5409 5624 5655 5834
Size: 466 59 33 89 1300 215 31 179
Cuts at: 5834 5918 5997 6158 6221 6245 6402 6488 1391
Size: 84 79 161 63 24 157 86 4072
Fragments arranged by size:
4072 1300 634 591 466 259 215 213
179 168 161 157 151 89 86 84
79 63 59 33 31 29 26 24
BbeI G_GCGC'C
Cuts at: 2370 2655 3647 2370
Size: 285 992 7892
Fragments arranged by size:
7892 992 285
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3702 4776 4987 928
Size: 971 1803 1074 211 5110
Fragments arranged by size:
5110 1803 1074 971 211
BbvCI CC'TCA_GC
Cuts at: 4397 5938 4397
Size: 1541 7628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4905 4939 5225 5259 6448 6482 7360
Size: 34 2968 34 286 34 1189 34 878
Cuts at: 7360 7394 1903
Size: 34 3678
Fragments arranged by size:
3678 2968 1189 878 286 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4328 7061 8271 4328
Size: 2733 1210 5226
Fragments arranged by size:
5226 2733 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2534 5760
Size: 107 122 71 631 605 337 525 3226
Cuts at: 5760 5782 6021 7780 136
Size: 22 239 1759 1525
Fragments arranged by size:
3226 1759 1525 631 605 525 337 239
122 107 71 22
BglII A'GATC_T
Cuts at: 5046 9164 5046
Size: 4118 5051
BlpI GC'TnA_GC
Cuts at: 2618 2816 2883 3121 3529 6586 2618
Size: 198 67 238 408 3057 5201
Fragments arranged by size:
5201 3057 408 238 198 67
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2855 2893 3268 3462 3928 3987 4020
Size: 235 830 38 375 194 466 59 33
Cuts at: 4020 5655 5834 5997 6031 6158 6221 6402 7230
Size: 1635 179 163 34 127 63 181 828
Cuts at: 7230 8386 1790
Size: 1156 2573
Fragments arranged by size:
2573 1635 1156 830 828 466 375 235
194 181 179 163 127 63 59 38
34 33
BmgBI CAC'GTC
Cuts at: 1318 2344 2464 4456 1318
Size: 1026 120 1992 6031
Fragments arranged by size:
6031 1992 1026 120
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2284 2464 4014
Size: 416 53 83 399 24 867 180 1550
Cuts at: 4014 4023 5806 5978 6262 6417 6900 7858 442
Size: 9 1783 172 284 155 483 958 1753
Fragments arranged by size:
1783 1753 1550 958 867 483 416 399
284 180 172 155 83 53 24 9
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9169
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3544 3544
Size: 9169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2165 2411 2626 2687 2818 2882 2883
Size: 857 69 246 215 61 131 64 1
Cuts at: 2883 3035 3375 3512 4093 4403 5186 5309 5352
Size: 152 340 137 581 310 783 123 43
Cuts at: 5352 5399 5780 6066 6123 7829 1239
Size: 47 381 286 57 1706 2579
Fragments arranged by size:
2579 1706 857 783 581 381 340 310
286 246 215 152 137 131 123 69
64 61 57 47 43 1
Bpu10I CC'TnA_GC
Cuts at: 2771 3041 3591 4397 5938 6159 2771
Size: 270 550 806 1541 221 5781
Fragments arranged by size:
5781 1541 806 550 270 221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3404 4121 7294 8159 8457 3404
Size: 717 3173 865 298 4116
Fragments arranged by size:
4116 3173 865 717 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2279 4118 4776 5433 7832 882
Size: 617 780 1839 658 657 2399 2219
Fragments arranged by size:
2399 2219 1839 780 658 657 617
BsaAI yAC'GTr
Cuts at: 493 1204 6960 493
Size: 711 5756 2702
Fragments arranged by size:
5756 2702 711
BsaBI GATnn'nnATC
Cuts at: 9163 9163
Size: 9169
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 2412 2652 3644
Size: 53 83 186 936 834 45 240 992
Cuts at: 3644 6976 7358 7993 275
Size: 3332 382 635 1451
Fragments arranged by size:
3332 1451 992 936 834 635 382 240
186 83 53 45
BsaWI w'CCGG_w
Cuts at: 2438 4357 4515 4629 7602 8000 8274 8421 8832
Size: 1919 158 114 2973 398 274 147 411
Cuts at: 8832 2438
Size: 2775
Fragments arranged by size:
2973 2775 1919 411 398 274 158 147
114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2306 2336 2375
Size: 30 165 30 597 30 276 30 39
Cuts at: 2375 2405 2629 2659 3008 3038 3276 3306 3380
Size: 30 224 30 349 30 238 30 74
Cuts at: 3380 3410 3464 3494 4124 4154 4475 4505 5835
Size: 30 54 30 630 30 321 30 1330
Cuts at: 5835 5865 1178
Size: 30 4482
Fragments arranged by size:
4482 1330 630 597 349 321 276 238
224 165 74 54 39 30 30 30
30 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2207 2945 2948 2951 3203 3485 4138
Size: 967 54 738 3 3 252 282 653
Cuts at: 4138 4480 4664 5588 5596 5686 1186
Size: 342 184 924 8 90 4669
Fragments arranged by size:
4669 967 924 738 653 342 282 252
184 90 54 8 3 3
BseYI C'CCAG_C
Cuts at: 2121 2494 3486 3602 3949 3964 3970 4031 4227
Size: 373 992 116 347 15 6 61 196
Cuts at: 4227 4609 4697 5195 5859 5886 5907 5911 6129
Size: 382 88 498 664 27 21 4 218
Cuts at: 6129 6212 8372 8868 2121
Size: 83 2160 496 2422
Fragments arranged by size:
2422 2160 992 664 498 496 382 373
347 218 196 116 88 83 61 27
21 15 6 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2189 2204 2465 4877 5273 6071 6371
Size: 573 453 15 261 2412 396 798 300
Cuts at: 6371 1163
Size: 3961
Fragments arranged by size:
3961 2412 798 573 453 396 300 261
15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6619 7380 8408 1540
Size: 275 186 4618 761 1028 2301
Fragments arranged by size:
4618 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2642 2777 4109 7230 7315 8386 2642
Size: 135 1332 3121 85 1071 3425
Fragments arranged by size:
3425 3121 1332 1071 135 85
BsmI GAATG_Cn'
Cuts at: 6680 6773 6680
Size: 93 9076
BsmBI CGTCTCn'nnnn_
Cuts at: 4446 5602 4446
Size: 1156 8013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2051 2642 2777 2855 2884 2893
Size: 399 235 26 591 135 78 29 9
Cuts at: 2893 3035 3268 3294 3462 3928 3987 4020 4109
Size: 142 233 26 168 466 59 33 89
Cuts at: 4109 5409 5624 5655 5834 5918 5997 6031 6158
Size: 1300 215 31 179 84 79 34 127
Cuts at: 6158 6221 6245 6402 6488 7230 7315 8386 1391
Size: 63 24 157 86 742 85 1071 2174
Fragments arranged by size:
2174 1300 1071 742 591 466 399 235
233 215 179 168 157 142 135 127
89 86 85 84 79 78 63 59
34 33 31 29 26 26 24 9
BspEI T'CCGG_A
Cuts at: 4515 4629 8832 4515
Size: 114 4203 4852
Fragments arranged by size:
4852 4203 114
BspHI T'CATG_A
Cuts at: 7058 8788 7058
Size: 1730 7439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2079 2193 2286 2487 2997 5238 6524 844
Size: 1235 114 93 201 510 2241 1286 3489
Fragments arranged by size:
3489 2241 1286 1235 510 201 114 93
BsrBI CCG'CTC
Cuts at: 1394 1823 2407 2425 2608 3109 3831 4336 4387
Size: 429 584 18 183 501 722 505 51
Cuts at: 4387 7056 1394
Size: 2669 3507
Fragments arranged by size:
3507 2669 722 584 505 501 429 183
51 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 5241 7664 7838 65
Size: 1183 3993 2423 174 1396
Fragments arranged by size:
3993 2423 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2438 2533 2654 2811 3276 3627 4184
Size: 110 628 95 121 157 465 351 557
Cuts at: 4184 4357 4401 4431 5208 6015 7813 8000 1700
Size: 173 44 30 777 807 1798 187 2869
Fragments arranged by size:
2869 1798 807 777 628 557 465 351
187 173 157 121 110 95 44 30
BsrGI T'GTAC_A
Cuts at: 96 4689 6114 96
Size: 4593 1425 3151
Fragments arranged by size:
4593 3151 1425
BssHII G'CGCG_C
Cuts at: 1925 2238 1925
Size: 313 8856
BssSI C'ACGA_G
Cuts at: 2486 4616 5084 7229 8241 2486
Size: 2130 468 2145 1012 3414
Fragments arranged by size:
3414 2145 2130 1012 468
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2110 794
Size: 1316 7853
BstBI TT'CG_AA
Cuts at: 7973 7973
Size: 9169
BstEII G'GTnAC_C
Cuts at: 1184 5020 1184
Size: 3836 5333
BstXI CCAn_nnnn'nTGG
Cuts at: 4027 4501 5744 5978 4027
Size: 474 1243 234 7218
Fragments arranged by size:
7218 1243 474 234
BstYI r'GATC_y
Cuts at: 1972 2039 4498 4951 5046 6224 6499 6866 7251
Size: 67 2459 453 95 1178 275 367 385
Cuts at: 7251 7268 8720 8806 8818 9164 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2459 1977 1452 1178 453 385 367 346
275 95 86 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 2918 2918
Size: 9169
BtgI C'CryG_G
Cuts at: 513 1065 2004 2061 2209 2233 2591 2600 2623
Size: 552 939 57 148 24 358 9 23
Cuts at: 2623 3255 3813 3889 4494 5755 5977 6376 6462
Size: 632 558 76 605 1261 222 399 86
Cuts at: 6462 513
Size: 3220
Fragments arranged by size:
3220 1261 939 632 605 558 552 399
358 222 148 86 76 57 24 23
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5415 5945 487
Size: 683 56 4189 530 3711
Fragments arranged by size:
4189 3711 683 530 56
BtsI GCAGTG_nn'
Cuts at: 2770 6104 6685 7479 7499 2770
Size: 3334 581 794 20 4440
Fragments arranged by size:
4440 3334 794 581 20
ClaI AT'CG_AT
Cuts at: 1554 3324 1554
Size: 1770 7399
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9134
DraI TTT'AAA
Cuts at: 6473 6473
Size: 9169
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8176 809
Size: 507 6860 1802
Fragments arranged by size:
6860 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2594 2691 5746
Size: 54 1425 50 275 18 764 97 3055
Cuts at: 5746 6017 6073 6230 6616 7505 9134 8
Size: 271 56 157 386 889 1629 43
Fragments arranged by size:
3055 1629 1425 889 764 386 275 271
157 97 56 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6616 1537
Size: 275 4804 4090
Fragments arranged by size:
4804 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4156 4298 5006 5111 5704 5877 7099 1353
Size: 2803 142 708 105 593 173 1222 3423
Fragments arranged by size:
3423 2803 1222 708 593 173 142 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2673 3068 5541 7756 8130 8276
Size: 1064 138 1084 395 2473 2215 374 146
Cuts at: 8276 387
Size: 1280
Fragments arranged by size:
2473 2215 1280 1084 1064 395 374 146
138
EcoICRI GAG'CTC
Cuts at: 2640 4107 2640
Size: 1467 7702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2165 2411 2626 2687 2818 2882
Size: 393 464 69 246 215 61 131 64
Cuts at: 2882 2883 3035 3375 3512 3526 3920 3978 4093
Size: 1 152 340 137 14 394 58 115
Cuts at: 4093 4403 4567 5186 5309 5352 5399 5780 6066
Size: 310 164 619 123 43 47 381 286
Cuts at: 6066 6123 6157 7232 7829 8616 8884 1239
Size: 57 34 1075 597 787 268 1524
Fragments arranged by size:
1524 1075 787 619 597 464 394 393
381 340 310 286 268 246 215 164
152 137 131 123 115 69 64 61
58 57 47 43 34 14 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2189 2189
Size: 9169
EcoO109I rG'GnC_Cy
Cuts at: 2021 2022 2227 2851 2852 2956 3458 3728 3894
Size: 1 205 624 1 104 502 270 166
Cuts at: 3894 3924 3925 3983 3984 5651 5652 5800 5831
Size: 30 1 58 1 1667 1 148 31
Cuts at: 5831 5993 5994 6154 6155 6217 6218 6399 2021
Size: 162 1 160 1 62 1 181 4791
Fragments arranged by size:
4791 1667 624 502 270 205 181 166
162 160 148 104 62 58 31 30
1 1 1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 2725 4418 5113 6478 6856 2725
Size: 1693 695 1365 378 5038
Fragments arranged by size:
5038 1693 1365 695 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5065 5671 5065
Size: 606 8563
FseI GG_CCGG'CC
Cuts at: 3631 6019 3631
Size: 2388 6781
FspI TGC'GCA
Cuts at: 7675 7675
Size: 9169
HaeII r_GCGC'y
Cuts at: 1678 2370 2655 2950 3647 8316 1678
Size: 692 285 295 697 4669 2531
Fragments arranged by size:
4669 2531 697 692 295 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2038 2070 2629
Size: 32 135 32 428 32 6 32 559
Cuts at: 2629 2661 3380 3412 3464 3496 3827 3859 4475
Size: 32 719 32 52 32 331 32 616
Cuts at: 4475 4507 4832 4864 5533 5565 5771 5803 7810
Size: 32 325 32 669 32 206 32 2007
Cuts at: 7810 7842 7884 7916 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2007 719 669 616 559 428 331
325 206 135 52 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4759 4916 6513 6759 669
Size: 988 281 2821 157 1597 246 3079
Fragments arranged by size:
3079 2821 1597 988 281 246 157
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9169
HpaI GTT'AAC
Cuts at: 6759 6759
Size: 9169
KasI G'GCGC_C
Cuts at: 2366 2651 3643 2366
Size: 285 992 7892
Fragments arranged by size:
7892 992 285
KpnI G_GTAC'C
Cuts at: 6494 6494
Size: 9169
MfeI C'AATT_G
Cuts at: 6768 6768
Size: 9169
MluI A'CGCG_T
Cuts at: 9045 9045
Size: 9169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3186 4019 4440 4861 5250 5262 5625 8283
Size: 1881 833 421 421 389 12 363 2658
Cuts at: 8283 8467 1305
Size: 184 2007
Fragments arranged by size:
2658 2007 1881 833 421 421 389 363
184 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5748 10
Size: 54 1425 343 3916 3431
Fragments arranged by size:
3916 3431 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3170 4358 5742 5976 7127 7486 7645
Size: 648 2004 1188 1384 234 1151 359 159
Cuts at: 7645 518
Size: 2042
Fragments arranged by size:
2042 2004 1384 1188 1151 648 359 234
159
MspA1I CmG'CkG
Cuts at: 1606 2110 2125 2155 2203 2211 2593 2602 2617
Size: 504 15 30 48 8 382 9 15
Cuts at: 2617 2780 2824 2887 3490 3859 4210 4591 4987
Size: 163 44 63 603 369 351 381 396
Cuts at: 4987 5911 6385 6585 7262 8410 8655 1606
Size: 924 474 200 677 1148 245 2120
Fragments arranged by size:
2120 1148 924 677 603 504 474 396
382 381 369 351 245 200 163 63
48 44 30 15 15 9 8
NaeI GCC'GGC
Cuts at: 2535 2656 3629 4403 6017 2535
Size: 121 973 774 1614 5687
Fragments arranged by size:
5687 1614 973 774 121
NarI GG'CG_CC
Cuts at: 2367 2652 3644 2367
Size: 285 992 7892
Fragments arranged by size:
7892 992 285
NcoI C'CATG_G
Cuts at: 513 1065 2004 3255 3889 4494 5977 6376 513
Size: 552 939 1251 634 605 1483 399 3306
Fragments arranged by size:
3306 1483 1251 939 634 605 552 399
NdeI CA'TA_TG
Cuts at: 387 3298 387
Size: 2911 6258
NgoMIV G'CCGG_C
Cuts at: 2533 2654 3627 4401 6015 2533
Size: 121 973 774 1614 5687
Fragments arranged by size:
5687 1614 973 774 121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9169
NotI GC'GGCC_GC
Cuts at: 6616 6616
Size: 9169
NruI TCG'CGA
Cuts at: 2508 2508
Size: 9169
NspI r_CATG'y
Cuts at: 1132 1229 3142 3169 4690 5101 5810 6527 8072
Size: 97 1913 27 1521 411 709 717 1545
Cuts at: 8072 1132
Size: 2229
Fragments arranged by size:
2229 1913 1545 1521 717 709 411 97
27
PasI CC'CwG_GG
Cuts at: 1348 2500 2877 3622 5990 1348
Size: 1152 377 745 2368 4527
Fragments arranged by size:
4527 2368 1152 745 377
PciI A'CATG_T
Cuts at: 4686 8068 4686
Size: 3382 5787
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4561 1108
Size: 3453 5716
PfoI T'CCnGG_A
Cuts at: 2427 2633 4380 5202 2427
Size: 206 1747 822 6394
Fragments arranged by size:
6394 1747 822 206
PmeI GTTT'AAAC
Cuts at: 6473 6473
Size: 9169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2276 2306 2308 2338 3276 3308 7238 7270 8751
Size: 30 2 30 938 32 3930 32 1481
Cuts at: 8751 8783 2276
Size: 32 2662
Fragments arranged by size:
3930 2662 1481 938 32 32 32 30
30 2
PpuMI rG'GwC_Cy
Cuts at: 2227 3728 3894 2227
Size: 1501 166 7502
Fragments arranged by size:
7502 1501 166
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9169
PsiI TTA'TAA
Cuts at: 6739 6739
Size: 9169
PspOMI G'GGCC_C
Cuts at: 2021 2851 3458 3924 3983 4016 5651 5830 5993
Size: 830 607 466 59 33 1635 179 163
Cuts at: 5993 6154 6217 6398 2021
Size: 161 63 181 4792
Fragments arranged by size:
4792 1635 830 607 466 181 179 163
161 63 59 33
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 9137
PstI C_TGCA'G
Cuts at: 830 2994 3884 4139 5152 6521 830
Size: 2164 890 255 1013 1369 3478
Fragments arranged by size:
3478 2164 1369 1013 890 255
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9169
PvuII CAG'CTG
Cuts at: 2110 2155 2203 2617 2824 3490 3859 5911 2110
Size: 45 48 414 207 666 369 2052 5368
Fragments arranged by size:
5368 2052 666 414 369 207 48 45
SacI G_AGCT'C
Cuts at: 2642 4109 2642
Size: 1467 7702
SacII CC_GC'GG
Cuts at: 2212 2594 2603 2212
Size: 382 9 8778
Fragments arranged by size:
8778 382 9
SalI G'TCGA_C
Cuts at: 1936 4914 6511 1936
Size: 2978 1597 4594
Fragments arranged by size:
4594 2978 1597
SanDI GG'GwC_CC
Cuts at: 2227 3728 3894 2227
Size: 1501 166 7502
Fragments arranged by size:
7502 1501 166
SapI GCTCTTCn'nnn_
Cuts at: 5111 5111
Size: 9169
SbfI CC_TGCA'GG
Cuts at: 2994 6521 2994
Size: 3527 5642
ScaI AGT'ACT
Cuts at: 7417 9129 7417
Size: 1712 7457
SexAI A'CCwGG_T
Cuts at: 5155 5155
Size: 9169
SfcI C'TryA_G
Cuts at: 826 1045 2990 3880 4135 5148 6517 7652 8333
Size: 219 1945 890 255 1013 1369 1135 681
Cuts at: 8333 8524 826
Size: 191 1471
Fragments arranged by size:
1945 1471 1369 1135 1013 890 681 255
219 191
SfoI GGC'GCC
Cuts at: 2368 2653 3645 2368
Size: 285 992 7892
Fragments arranged by size:
7892 992 285
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9169
SgrAI Cr'CCGG_yG
Cuts at: 1700 2654 4357 1700
Size: 954 1703 6512
Fragments arranged by size:
6512 1703 954
SmaI CCC'GGG
Cuts at: 1872 2028 2037 2253 2339 2544 3692 3733 6496
Size: 156 9 216 86 205 1148 41 2763
Cuts at: 6496 1872
Size: 4545
Fragments arranged by size:
4545 2763 1148 216 205 156 86 41
9
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3383 4100 7273 8174 8436 820
Size: 197 926 1440 717 3173 901 262 1553
Fragments arranged by size:
3173 1553 1440 926 901 717 262 197
SnaBI TAC'GTA
Cuts at: 493 6960 493
Size: 6467 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9169
SphI G_CATG'C
Cuts at: 1132 5810 6527 1132
Size: 4678 717 3774
Fragments arranged by size:
4678 3774 717
SrfI GCCC'GGGC
Cuts at: 2253 2253
Size: 9169
SspI AAT'ATT
Cuts at: 5 52 7093 5
Size: 47 7041 2081
Fragments arranged by size:
7041 2081 47
StuI AGG'CCT
Cuts at: 1517 4395 5687 1517
Size: 2878 1292 4999
Fragments arranged by size:
4999 2878 1292
StyI C'CwwG_G
Cuts at: 513 1065 2004 2952 3255 3889 4423 4494 5340
Size: 552 939 948 303 634 534 71 846
Cuts at: 5340 5821 5977 6009 6376 6435 6608 513
Size: 481 156 32 367 59 173 3074
Fragments arranged by size:
3074 948 939 846 634 552 534 481
367 303 173 156 71 59 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2183 6046 6198 6307 6379 7196 7213
Size: 1255 603 3863 152 109 72 817 17
Cuts at: 7213 7366 7551 7996 325
Size: 153 185 445 1498
Fragments arranged by size:
3863 1498 1255 817 603 445 185 153
152 109 72 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4689 5577 6114
Size: 274 80 33 51 1443 2712 888 537
Cuts at: 6114 7415 9127 96
Size: 1301 1712 138
Fragments arranged by size:
2712 1712 1443 1301 888 537 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5835 5867 9129 9161 5835
Size: 32 3262 32 5843
Fragments arranged by size:
5843 3262 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9169
XbaI T'CTAG_A
Cuts at: 6505 6505
Size: 9169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2621 2842 5741 5756 2621
Size: 221 2899 15 6034
Fragments arranged by size:
6034 2899 221 15
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9169
XmaI C'CCGG_G
Cuts at: 1870 2026 2035 2251 2337 2542 3690 3731 6494
Size: 156 9 216 86 205 1148 41 2763
Cuts at: 6494 1870
Size: 4545
Fragments arranged by size:
4545 2763 1148 216 205 156 86 41
9
XmnI GAAnn'nnTTC
Cuts at: 4442 7298 4442
Size: 2856 6313
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6977 276
Size: 53 83 186 936 5443 2468
Fragments arranged by size:
5443 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FspAI
NsiI PacI PmlI PspXI RsrII SfiI SwaI