Restriction map for
pFN21AA0521
(Circular) (Six-base) MAPSORT of: pFN21AA0521.seq Check: 4127 from: 1 to: 7750
pFN21AA0521 7750 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3856 3856
Size: 7750
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5040 5560 278
Size: 53 83 186 936 3504 520 2468
Fragments arranged by size:
3504 2468 936 520 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4058 5093 1937
Size: 2121 1035 4594
Fragments arranged by size:
4594 2121 1035
Acc65I G'GTAC_C
Cuts at: 5071 5071
Size: 7750
AclI AA'CG_TT
Cuts at: 1563 5877 6250 1563
Size: 4314 373 3063
Fragments arranged by size:
4314 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2396 2654 2975 2993 3337 3956 4787 5813
Size: 764 258 321 18 344 619 831 1026
Cuts at: 5813 7197 7465 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1026 831 764 619 344 321
268 258 18
AfeI AGC'GCT
Cuts at: 4172 4739 4172
Size: 567 7183
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7553
AflIII A'CryG_T
Cuts at: 1203 4061 6649 7626 1203
Size: 2858 2588 977 1327
Fragments arranged by size:
2858 2588 1327 977
AgeI A'CCGG_T
Cuts at: 6581 6581
Size: 7750
AhdI GACnn_n'nnGTC
Cuts at: 2143 2315 2956 6479 2143
Size: 172 641 3523 3414
Fragments arranged by size:
3523 3414 641 172
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7750
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4035 1184
Size: 2851 4899
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2396 2428 4028 4060 2396
Size: 32 1600 32 6086
Fragments arranged by size:
6086 1600 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3619 3949 4207 4777 4780 4883 5493 7065 3619
Size: 330 258 570 3 103 610 1572 4304
Fragments arranged by size:
4304 1572 610 570 330 258 103 3
ApaI G_GGCC'C
Cuts at: 2395 3351 4499 2395
Size: 956 1148 5646
Fragments arranged by size:
5646 1148 956
ApaLI G'TGCA_C
Cuts at: 2345 5807 6963 2345
Size: 3462 1156 3132
Fragments arranged by size:
3462 3132 1156
ApoI r'AATT_y
Cuts at: 1493 2117 2684 5059 5285 5437 7546 1493
Size: 624 567 2375 226 152 2109 1697
Fragments arranged by size:
2375 2109 1697 624 567 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6304 160
Size: 872 5272 1606
Fragments arranged by size:
5272 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7750
AvaI C'yCGr_G
Cuts at: 1870 1943 3525 3783 4500 4550 4897 5075 1870
Size: 73 1582 258 717 50 347 178 4545
Fragments arranged by size:
4545 1582 717 347 258 178 73 50
BamHI G'GATC_C
Cuts at: 3939 5080 5447 3939
Size: 1141 367 6242
Fragments arranged by size:
6242 1141 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3877 5071 6526 618
Size: 325 217 627 2090 1194 1455 1842
Fragments arranged by size:
2090 1842 1455 1194 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2048 2395 2985 3275 3351 3851 4499 4733
Size: 657 347 590 290 76 500 648 234
Cuts at: 4733 4751 4841 4904 5069 1391
Size: 18 90 63 165 4072
Fragments arranged by size:
4072 657 648 590 500 347 290 234
165 90 76 63 18
BbeI G_GCGC'C
Cuts at: 3881 3881
Size: 7750
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2847 2952 3006 3053 3516 3666 5040
Size: 971 948 105 54 47 463 150 1374
Cuts at: 5040 928
Size: 3638
Fragments arranged by size:
3638 1374 971 948 463 150 105 54
47
BbvCI CC'TCA_GC
Cuts at: 2204 2204
Size: 7750
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5941 5975 1903
Size: 34 4004 34 3678
Fragments arranged by size:
4004 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5642 6852 5642
Size: 1210 6540
BclI T'GATC_A
Cuts at: 1548 2697 3225 1548
Size: 1149 528 6073
Fragments arranged by size:
6073 1149 528
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 3299 6361
Size: 107 122 71 631 605 380 1247 3062
Cuts at: 6361 136
Size: 1525
Fragments arranged by size:
3062 1525 1247 631 605 380 122 107
71
BglII A'GATC_T
Cuts at: 3474 7745 3474
Size: 4271 3479
BlpI GC'TnA_GC
Cuts at: 4622 4750 4835 5167 4622
Size: 128 85 332 7205
Fragments arranged by size:
7205 332 128 85
Bme1580I G_kGCm'C
Cuts at: 1790 2349 2395 3351 4499 4611 4955 5811 6967
Size: 559 46 956 1148 112 344 856 1156
Cuts at: 6967 1790
Size: 2573
Fragments arranged by size:
2573 1156 1148 956 856 559 344 112
46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7750
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2780 5481 6439
Size: 416 53 83 399 24 1363 2701 958
Cuts at: 6439 442
Size: 1753
Fragments arranged by size:
2701 1753 1363 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7750
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4831 4951 4831
Size: 120 7630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2272 2371 2483 2537 3047 3503 3938 4100
Size: 1033 99 112 54 510 456 435 162
Cuts at: 4100 4154 4229 4292 4313 4346 4397 4511 6410
Size: 54 75 63 21 33 51 114 1899
Cuts at: 6410 1239
Size: 2579
Fragments arranged by size:
2579 1899 1033 510 456 435 162 114
112 99 75 63 54 54 51 33
21
Bpu10I CC'TnA_GC
Cuts at: 2204 2204
Size: 7750
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2348 2477 2957 3566 4379 4925 5875 6740 7038
Size: 129 480 609 813 546 950 865 298
Cuts at: 7038 2348
Size: 3060
Fragments arranged by size:
3060 950 865 813 609 546 480 298
129
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2017 3551 3692 6413 882
Size: 617 518 1534 141 2721 2219
Fragments arranged by size:
2721 2219 1534 617 518 141
BsaAI yAC'GTr
Cuts at: 493 1204 2353 5541 493
Size: 711 1149 3188 2702
Fragments arranged by size:
3188 2702 1149 711
BsaBI GATnn'nnATC
Cuts at: 7744 7744
Size: 7750
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2892 3878 5022 5037
Size: 53 83 186 936 1359 986 1144 15
Cuts at: 5037 5557 5939 6574 275
Size: 520 382 635 1451
Fragments arranged by size:
1451 1359 1144 986 936 635 520 382
186 83 53 15
BsaWI w'CCGG_w
Cuts at: 2532 6183 6581 6855 7002 7413 2532
Size: 3651 398 274 147 411 2869
Fragments arranged by size:
3651 2869 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 3257 3287 3441
Size: 30 165 30 993 30 831 30 154
Cuts at: 3441 3471 4028 4058 4907 4937 1178
Size: 30 557 30 849 30 3991
Fragments arranged by size:
3991 993 849 831 557 165 154 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2221 2417 2534 3221 3353 3356 4133 1186
Size: 1035 196 117 687 132 3 777 4803
Fragments arranged by size:
4803 1035 777 687 196 132 117 3
BseYI C'CCAG_C
Cuts at: 2031 3519 3634 3954 4173 4344 4449 4469 4740
Size: 1488 115 320 219 171 105 20 271
Cuts at: 4740 4975 6953 7449 2031
Size: 235 1978 496 2332
Fragments arranged by size:
2332 1978 1488 496 320 271 235 219
171 115 105 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2762 3703 4679 1163
Size: 573 1026 941 976 4234
Fragments arranged by size:
4234 1026 976 941 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4656 5200 5961 6989 1540
Size: 275 186 2655 544 761 1028 2301
Fragments arranged by size:
2655 2301 1028 761 544 275 186
BsiHKAI G_wGCw'C
Cuts at: 2349 3275 3851 4751 4814 5811 5896 6967 2349
Size: 926 576 900 63 997 85 1071 3132
Fragments arranged by size:
3132 1071 997 926 900 576 85 63
BsmI GAATG_Cn'
Cuts at: 2751 5261 5354 2751
Size: 2510 93 5147
Fragments arranged by size:
5147 2510 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2459 4001 2459
Size: 1542 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2349 2395 2985 3275 3351 3851
Size: 399 258 301 46 590 290 76 500
Cuts at: 3851 4499 4611 4733 4751 4814 4841 4904 4955
Size: 648 112 122 18 63 27 63 51
Cuts at: 4955 5069 5811 5896 6967 1391
Size: 114 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 648 590 500 399 301
290 258 122 114 112 85 76 63
63 51 46 27 18
BspEI T'CCGG_A
Cuts at: 7413 7413
Size: 7750
BspHI T'CATG_A
Cuts at: 5639 7369 5639
Size: 1730 6020
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2466 3522 3856 4080 5105 844
Size: 1622 1056 334 224 1025 3489
Fragments arranged by size:
3489 1622 1056 1025 334 224
BsrBI CCG'CTC
Cuts at: 1394 1823 3421 4033 4090 4267 4282 4330 4366
Size: 429 1598 612 57 177 15 48 36
Cuts at: 4366 4830 5637 1394
Size: 464 807 3507
Fragments arranged by size:
3507 1598 807 612 464 429 177 57
48 36 15
BsrDI GCAATG_nn'
Cuts at: 65 1248 6245 6419 65
Size: 1183 4997 174 1396
Fragments arranged by size:
4997 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4587 4993 6394 6581 1700
Size: 110 2777 406 1401 187 2869
Fragments arranged by size:
2869 2777 1401 406 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7750
BssHII G'CGCG_C
Cuts at: 1925 4123 4221 4419 1925
Size: 2198 98 198 5256
Fragments arranged by size:
5256 2198 198 98
BssSI C'ACGA_G
Cuts at: 2231 3268 3397 4160 5810 6822 2231
Size: 1037 129 763 1650 1012 3159
Fragments arranged by size:
3159 1650 1037 1012 763 129
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2353 3039 4777 4780 794
Size: 1559 686 1738 3 3764
Fragments arranged by size:
3764 1738 1559 686 3
BstBI TT'CG_AA
Cuts at: 6554 6554
Size: 7750
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7750
BstXI CCAn_nnnn'nTGG
Cuts at: 3916 4681 3916
Size: 765 6985
BstYI r'GATC_y
Cuts at: 1972 2185 3474 3939 4668 5080 5447 5832 5849
Size: 213 1289 465 729 412 367 385 17
Cuts at: 5849 7301 7387 7399 7745 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1289 729 465 412 385 367
346 213 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2927 2927
Size: 7750
BtgI C'CryG_G
Cuts at: 513 1065 2262 3538 4353 513
Size: 552 1197 1276 815 3910
Fragments arranged by size:
3910 1276 1197 815 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3230 4617 487
Size: 683 56 2004 1387 3620
Fragments arranged by size:
3620 2004 1387 683 56
BtsI GCAGTG_nn'
Cuts at: 2533 4988 5266 6060 6080 2533
Size: 2455 278 794 20 4203
Fragments arranged by size:
4203 2455 794 278 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7750
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7715
DraI TTT'AAA
Cuts at: 2115 5054 2115
Size: 2939 4811
DraIII CAC_nnn'GTG
Cuts at: 2353 2353
Size: 7750
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2897 6757 809
Size: 507 1581 3860 1802
Fragments arranged by size:
3860 1802 1581 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2227 2431 2632
Size: 54 1425 50 275 18 397 204 201
Cuts at: 2632 3198 3489 3588 3649 4440 4593 4653 4995
Size: 566 291 99 61 791 153 60 342
Cuts at: 4995 5197 6086 7715 8
Size: 202 889 1629 43
Fragments arranged by size:
1629 1425 889 791 566 397 342 291
275 204 202 201 153 99 61 60
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4653 5197 1537
Size: 275 2841 544 4090
Fragments arranged by size:
4090 2841 544 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2444 3360 5680 1353
Size: 1091 916 2320 3423
Fragments arranged by size:
3423 2320 1091 916
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3671 6337 6711 6857 387
Size: 1064 138 2082 2666 374 146 1280
Fragments arranged by size:
2666 2082 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3273 3849 4749 3273
Size: 576 900 6274
Fragments arranged by size:
6274 900 576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2272 2371 2396 2483 2537 2654 2975
Size: 393 640 99 25 87 54 117 321
Cuts at: 2975 2993 3047 3337 3503 3938 3956 4100 4154
Size: 18 54 290 166 435 18 144 54
Cuts at: 4154 4229 4292 4313 4346 4397 4511 4787 5813
Size: 75 63 21 33 51 114 276 1026
Cuts at: 5813 6410 7197 7465 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1026 787 640 597 435 393 321
290 276 268 166 144 117 114 99
87 75 63 54 54 54 51 33
25 21 18 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2203 2399 2519 2849 2203
Size: 196 120 330 7104
Fragments arranged by size:
7104 330 196 120
EcoO109I rG'GnC_Cy
Cuts at: 2027 2391 2392 3347 3348 3547 3780 3797 4495
Size: 364 1 955 1 199 233 17 698
Cuts at: 4495 4496 2027
Size: 1 5281
Fragments arranged by size:
5281 955 698 364 233 199 17 1
1 1
EcoRI G'AATT_C
Cuts at: 5059 5437 5059
Size: 378 7372
EcoRV GAT'ATC
Cuts at: 2875 3073 2875
Size: 198 7552
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2336 3023 2336
Size: 687 7063
FspI TGC'GCA
Cuts at: 6256 6256
Size: 7750
HaeII r_GCGC'y
Cuts at: 1678 2503 2926 3064 3234 3645 3881 4174 4189
Size: 825 423 138 170 411 236 293 15
Cuts at: 4189 4300 4306 4375 4549 4575 4629 4741 5013
Size: 111 6 69 174 26 54 112 272
Cuts at: 5013 6897 1678
Size: 1884 2531
Fragments arranged by size:
2531 1884 825 423 411 293 272 236
174 170 138 112 111 69 54 26
15 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2129 2161 2204
Size: 32 135 32 427 32 98 32 43
Cuts at: 2204 2236 2301 2333 2864 2896 2942 2974 4907
Size: 32 65 32 531 32 46 32 1933
Cuts at: 4907 4939 6391 6423 6465 6497 1373
Size: 32 1452 32 42 32 2626
Fragments arranged by size:
2626 1933 1452 531 427 135 98 65
46 43 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4059 5094 5340 669
Size: 988 281 2121 1035 246 3079
Fragments arranged by size:
3079 2121 1035 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7750
HpaI GTT'AAC
Cuts at: 5340 5340
Size: 7750
KasI G'GCGC_C
Cuts at: 3877 3877
Size: 7750
KpnI G_GTAC'C
Cuts at: 5075 5075
Size: 7750
MfeI C'AATT_G
Cuts at: 5349 5349
Size: 7750
MluI A'CGCG_T
Cuts at: 4061 7626 4061
Size: 3565 4185
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3903 6864 7048 1305
Size: 2598 2961 184 2007
Fragments arranged by size:
2961 2598 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2634 3200 4595 10
Size: 54 1425 343 802 566 1395 3165
Fragments arranged by size:
3165 1425 1395 802 566 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5708 6067 6226 518
Size: 648 4542 359 159 2042
Fragments arranged by size:
4542 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2132 3013 3442 3937 3958 4015 4114 4153
Size: 526 881 429 495 21 57 99 39
Cuts at: 4153 4618 4777 4913 5166 5843 6991 7236 1606
Size: 465 159 136 253 677 1148 245 2120
Fragments arranged by size:
2120 1148 881 677 526 495 465 429
253 245 159 136 99 57 39 21
NaeI GCC'GGC
Cuts at: 4589 4995 4589
Size: 406 7344
NarI GG'CG_CC
Cuts at: 3878 3878
Size: 7750
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7198
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7750
NgoMIV G'CCGG_C
Cuts at: 4587 4993 4587
Size: 406 7344
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7750
NotI GC'GGCC_GC
Cuts at: 5197 5197
Size: 7750
NspI r_CATG'y
Cuts at: 1132 1229 3007 3037 4513 5108 6653 1132
Size: 97 1778 30 1476 595 1545 2229
Fragments arranged by size:
2229 1778 1545 1476 595 97 30
PasI CC'CwG_GG
Cuts at: 1348 2057 1348
Size: 709 7041
PciI A'CATG_T
Cuts at: 6649 6649
Size: 7750
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4207 4883 1108
Size: 3099 676 3975
Fragments arranged by size:
3975 3099 676
PfoI T'CCnGG_A
Cuts at: 2249 3405 3915 4026 2249
Size: 1156 510 111 5973
Fragments arranged by size:
5973 1156 510 111
PmeI GTTT'AAAC
Cuts at: 5054 5054
Size: 7750
PmlI CAC'GTG
Cuts at: 2353 2353
Size: 7750
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2396 2428 4028 4060 4814 4846 5819 5851 7332
Size: 32 1600 32 754 32 973 32 1481
Cuts at: 7332 7364 2396
Size: 32 2782
Fragments arranged by size:
2782 1600 1481 973 754 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2027 3547 2027
Size: 1520 6230
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7750
PsiI TTA'TAA
Cuts at: 5320 5320
Size: 7750
PspOMI G'GGCC_C
Cuts at: 2391 3347 4495 2391
Size: 956 1148 5646
Fragments arranged by size:
5646 1148 956
PstI C_TGCA'G
Cuts at: 830 2400 2412 3567 3629 4056 4146 4233 5102
Size: 1570 12 1155 62 427 90 87 869
Cuts at: 5102 830
Size: 3478
Fragments arranged by size:
3478 1570 1155 869 427 90 87 62
12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7750
PvuII CAG'CTG
Cuts at: 2132 3442 3958 4153 4618 4777 4913 2132
Size: 1310 516 195 465 159 136 4969
Fragments arranged by size:
4969 1310 516 465 195 159 136
SacI G_AGCT'C
Cuts at: 3275 3851 4751 3275
Size: 576 900 6274
Fragments arranged by size:
6274 900 576
SalI G'TCGA_C
Cuts at: 1936 4057 5092 1936
Size: 2121 1035 4594
Fragments arranged by size:
4594 2121 1035
SanDI GG'GwC_CC
Cuts at: 2027 3547 2027
Size: 1520 6230
SbfI CC_TGCA'GG
Cuts at: 2400 3567 5102 2400
Size: 1167 1535 5048
Fragments arranged by size:
5048 1535 1167
ScaI AGT'ACT
Cuts at: 5998 7710 5998
Size: 1712 6038
SfcI C'TryA_G
Cuts at: 826 1045 2396 2408 3563 3625 4052 4142 4229
Size: 219 1351 12 1155 62 427 90 87
Cuts at: 4229 5098 6233 6914 7105 826
Size: 869 1135 681 191 1471
Fragments arranged by size:
1471 1351 1155 1135 869 681 427 219
191 90 87 62 12
SfoI GGC'GCC
Cuts at: 3879 3879
Size: 7750
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7750
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7750
SmaI CCC'GGG
Cuts at: 1872 5077 1872
Size: 3205 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2363 2492 2972 3581 4394 4940
Size: 197 926 420 129 480 609 813 546
Cuts at: 4940 5854 6755 7017 820
Size: 914 901 262 1553
Fragments arranged by size:
1553 926 914 901 813 609 546 480
420 262 197 129
SnaBI TAC'GTA
Cuts at: 493 5541 493
Size: 5048 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7750
SphI G_CATG'C
Cuts at: 1132 3037 4513 5108 1132
Size: 1905 1476 595 3774
Fragments arranged by size:
3774 1905 1476 595
SspI AAT'ATT
Cuts at: 5 52 5674 5
Size: 47 5622 2081
Fragments arranged by size:
5622 2081 47
StuI AGG'CCT
Cuts at: 1517 2855 3531 4310 1517
Size: 1338 676 779 4957
Fragments arranged by size:
4957 1338 779 676
StyI C'CwwG_G
Cuts at: 513 1065 2895 4695 5013 5189 513
Size: 552 1830 1800 318 176 3074
Fragments arranged by size:
3074 1830 1800 552 318 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3107 5777 5794 5947 6132 6577 325
Size: 1255 1527 2670 17 153 185 445 1498
Fragments arranged by size:
2670 1527 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2382 5996 7708
Size: 274 80 33 51 1443 405 3614 1712
Cuts at: 7708 96
Size: 138
Fragments arranged by size:
3614 1712 1443 405 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3441 3473 7710 7742 3441
Size: 32 4237 32 3449
Fragments arranged by size:
4237 3449 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3949 4860 1316
Size: 2633 911 4206
Fragments arranged by size:
4206 2633 911
XbaI T'CTAG_A
Cuts at: 5086 5086
Size: 7750
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7750
XmaI C'CCGG_G
Cuts at: 1870 5075 1870
Size: 3205 4545
XmnI GAAnn'nnTTC
Cuts at: 4043 5879 4043
Size: 1836 5914
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5038 5558 276
Size: 53 83 186 936 3504 520 2468
Fragments arranged by size:
3504 2468 936 520 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI FspAI
NruI NsiI PacI PspXI PsrI RsrII SacII SapI
SexAI SfiI SrfI SwaI XcmI