Restriction map for
pFN21AA0512
(Circular) (Six-base) MAPSORT of: pFN21AA0512.seq Check: 8617 from: 1 to: 6601
pFN21AA0512 6601 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3525 3525
Size: 6601
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4411 278
Size: 53 83 186 936 2875 2468
Fragments arranged by size:
2875 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2076 2916 3944 1937
Size: 139 840 1028 4594
Fragments arranged by size:
4594 1028 840 139
Acc65I G'GTAC_C
Cuts at: 2836 3922 2836
Size: 1086 5515
AclI AA'CG_TT
Cuts at: 1563 4728 5101 1563
Size: 3165 373 3063
Fragments arranged by size:
3165 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2256 2643 3173 3383 4664 6048 6316 1632
Size: 624 387 530 210 1281 1384 268 1917
Fragments arranged by size:
1917 1384 1281 624 530 387 268 210
AfeI AGC'GCT
Cuts at: 2317 2317
Size: 6601
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6404
AflIII A'CryG_T
Cuts at: 1203 5500 6477 1203
Size: 4297 977 1327
Fragments arranged by size:
4297 1327 977
AgeI A'CCGG_T
Cuts at: 5432 5432
Size: 6601
AhdI GACnn_n'nnGTC
Cuts at: 2200 2589 3049 5330 2200
Size: 389 460 2281 3471
Fragments arranged by size:
3471 2281 460 389
AleI CACnn'nnGTG
Cuts at: 1166 2579 1166
Size: 1413 5188
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6601
AlwNI CAG_nnn'CTG
Cuts at: 2828 2963 2966 2975 3065 4344 5916 2828
Size: 135 3 9 90 1279 1572 3513
Fragments arranged by size:
3513 1572 1279 135 90 9 3
ApaI G_GGCC'C
Cuts at: 2254 2254
Size: 6601
ApaLI G'TGCA_C
Cuts at: 4658 5814 4658
Size: 1156 5445
ApoI r'AATT_y
Cuts at: 1493 3476 3604 3773 3896 3910 4136 4288 6397
Size: 1983 128 169 123 14 226 152 2109
Cuts at: 6397 1493
Size: 1697
Fragments arranged by size:
2109 1983 1697 226 169 152 128 123
14
AseI AT'TA_AT
Cuts at: 160 1032 3708 5155 160
Size: 872 2676 1447 1606
Fragments arranged by size:
2676 1606 1447 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
AvaI C'yCGr_G
Cuts at: 1870 1943 2432 2630 3071 3926 1870
Size: 73 489 198 441 855 4545
Fragments arranged by size:
4545 855 489 441 198 73
BamHI G'GATC_C
Cuts at: 3931 4298 3931
Size: 367 6234
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2533 2551 2836 3922
Size: 325 217 627 273 473 18 285 1086
Cuts at: 3922 5377 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1086 627 473 325 285 273
217 18
BanII G_rGCy'C
Cuts at: 1391 2254 2485 2794 3072 3920 1391
Size: 863 231 309 278 848 4072
Fragments arranged by size:
4072 863 848 309 278 231
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3106 928
Size: 971 1207 4423
Fragments arranged by size:
4423 1207 971
BbvCI CC'TCA_GC
Cuts at: 2289 2319 2658 2289
Size: 30 339 6232
Fragments arranged by size:
6232 339 30
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4792 4826 1903
Size: 34 2855 34 3678
Fragments arranged by size:
3678 2855 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4493 5703 4493
Size: 1210 5391
BclI T'GATC_A
Cuts at: 1548 3312 1548
Size: 1764 4837
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2354 2453 3353
Size: 107 122 71 631 605 682 99 900
Cuts at: 3353 5212 136
Size: 1859 1525
Fragments arranged by size:
1859 1525 900 682 631 605 122 107
99 71
BglII A'GATC_T
Cuts at: 6596 6596
Size: 6601
BlpI GC'TnA_GC
Cuts at: 4018 4018
Size: 6601
Bme1580I G_kGCm'C
Cuts at: 1790 2254 2673 4662 5818 1790
Size: 464 419 1989 1156 2573
Fragments arranged by size:
2573 1989 1156 464 419
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6601
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2219 2694 2712
Size: 416 53 83 399 24 802 475 18
Cuts at: 2712 2808 2933 4332 5290 442
Size: 96 125 1399 958 1753
Fragments arranged by size:
1753 1399 958 802 475 416 399 125
96 83 53 24 18
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2172 2850 2964 3834 5261 1239
Size: 933 678 114 870 1427 2579
Fragments arranged by size:
2579 1427 933 870 678 114
Bpu10I CC'TnA_GC
Cuts at: 2289 2319 2658 3841 2289
Size: 30 339 1183 5049
Fragments arranged by size:
5049 1183 339 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4726 5591 5889 4726
Size: 865 298 5438
Fragments arranged by size:
5438 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2091 3068 5264 882
Size: 617 592 977 2196 2219
Fragments arranged by size:
2219 2196 977 617 592
BsaAI yAC'GTr
Cuts at: 493 1204 3688 4392 493
Size: 711 2484 704 2702
Fragments arranged by size:
2702 2484 711 704
BsaBI GATnn'nnATC
Cuts at: 6595 6595
Size: 6601
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4408 4790 5425 275
Size: 53 83 186 936 2875 382 635 1451
Fragments arranged by size:
2875 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5034 5432 5706 5853 6264 5034
Size: 398 274 147 411 5371
Fragments arranged by size:
5371 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2355 2385 3020 3050 3807
Size: 30 165 30 952 30 635 30 757
Cuts at: 3807 3837 1178
Size: 30 3942
Fragments arranged by size:
3942 952 757 635 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6601
BseYI C'CCAG_C
Cuts at: 2144 2168 2302 3078 5804 6300 2144
Size: 24 134 776 2726 496 2445
Fragments arranged by size:
2726 2445 776 496 134 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6028
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4051 4812 5840 1540
Size: 275 186 2050 761 1028 2301
Fragments arranged by size:
2301 2050 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4662 4747 5818 4662
Size: 85 1071 5445
Fragments arranged by size:
5445 1071 85
BsmI GAATG_Cn'
Cuts at: 4112 4205 4112
Size: 93 6508
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2254 2485 2673 2794 3072 3920 4662
Size: 399 464 231 188 121 278 848 742
Cuts at: 4662 4747 5818 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 848 742 464 399 278 231
188 121 85
BspEI T'CCGG_A
Cuts at: 6264 6264
Size: 6601
BspHI T'CATG_A
Cuts at: 4490 6220 4490
Size: 1730 4871
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3525 3956 844
Size: 2681 431 3489
Fragments arranged by size:
3489 2681 431
BsrBI CCG'CTC
Cuts at: 1394 1823 2834 4488 1394
Size: 429 1011 1654 3507
Fragments arranged by size:
3507 1654 1011 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2412 2478 3531 5096 5270 65
Size: 1183 1164 66 1053 1565 174 1396
Fragments arranged by size:
1565 1396 1183 1164 1053 174 66
BsrFI r'CCGG_y
Cuts at: 1700 1810 3393 5245 5432 1700
Size: 110 1583 1852 187 2869
Fragments arranged by size:
2869 1852 1583 187 110
BsrGI T'GTAC_A
Cuts at: 96 3405 96
Size: 3309 3292
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6601
BssSI C'ACGA_G
Cuts at: 4661 5673 4661
Size: 1012 5589
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2963 2966 2975 794
Size: 2169 3 9 4420
Fragments arranged by size:
4420 2169 9 3
BstBI TT'CG_AA
Cuts at: 3602 5405 3602
Size: 1803 4798
BstEII G'GTnAC_C
Cuts at: 1184 2581 1184
Size: 1397 5204
BstYI r'GATC_y
Cuts at: 1972 3931 4298 4683 4700 6152 6238 6250 6596
Size: 1959 367 385 17 1452 86 12 346
Cuts at: 6596 1972
Size: 1977
Fragments arranged by size:
1977 1959 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2379 2379
Size: 6601
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6049
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2625 487
Size: 683 56 1399 4463
Fragments arranged by size:
4463 1399 683 56
BtsI GCAGTG_nn'
Cuts at: 2301 4117 4911 4931 2301
Size: 1816 794 20 3971
Fragments arranged by size:
3971 1816 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6601
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6566
DraI TTT'AAA
Cuts at: 3905 3905
Size: 6601
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5608 809
Size: 507 4292 1802
Fragments arranged by size:
4292 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 3354 4048
Size: 54 1425 50 275 18 288 1236 694
Cuts at: 4048 4937 6566 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1236 889 694 288 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4048 1537
Size: 275 2236 4090
Fragments arranged by size:
4090 2236 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2105 4531 1353
Size: 752 2426 3423
Fragments arranged by size:
3423 2426 752
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3000 5188 5562 5708 387
Size: 1064 138 1411 2188 374 146 1280
Fragments arranged by size:
2188 1411 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2172 2256 2643 2850 2964 3173 3383
Size: 393 540 84 387 207 114 209 210
Cuts at: 3383 3834 4664 5261 6048 6316 1239
Size: 451 830 597 787 268 1524
Fragments arranged by size:
1524 830 787 597 540 451 393 387
268 210 209 207 114 84
EcoNI CCTnn'n_nnAGG
Cuts at: 3002 3002
Size: 6601
EcoO109I rG'GnC_Cy
Cuts at: 2250 2446 2591 2868 3007 3175 2250
Size: 196 145 277 139 168 5676
Fragments arranged by size:
5676 277 196 168 145 139
EcoRI G'AATT_C
Cuts at: 3773 3910 4288 3773
Size: 137 378 6086
Fragments arranged by size:
6086 378 137
EcoRV GAT'ATC
Cuts at: 3430 3430
Size: 6601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3142 3806 3142
Size: 664 5937
FspI TGC'GCA
Cuts at: 5107 5107
Size: 6601
HaeII r_GCGC'y
Cuts at: 1678 2319 3124 5748 1678
Size: 641 805 2624 2531
Fragments arranged by size:
2624 2531 805 641
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2175 2186 2207 2218 2249
Size: 32 135 32 603 11 21 11 31
Cuts at: 2249 2281 2575 2607 3035 3047 3067 3079 5242
Size: 32 294 32 428 12 20 12 2163
Cuts at: 5242 5274 5316 5348 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2163 603 428 294 135 42 32
32 32 32 32 32 31 21 20
12 12 11 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3945 4191 669
Size: 988 281 2007 246 3079
Fragments arranged by size:
3079 2007 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6601
HpaI GTT'AAC
Cuts at: 4191 4191
Size: 6601
KpnI G_GTAC'C
Cuts at: 2840 3926 2840
Size: 1086 5515
MfeI C'AATT_G
Cuts at: 4200 4200
Size: 6601
MluI A'CGCG_T
Cuts at: 6477 6477
Size: 6601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2263 3238 3448 5715 5899 1305
Size: 958 975 210 2267 184 2007
Fragments arranged by size:
2267 2007 975 958 210 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2120 3356 10
Size: 54 1425 343 288 1236 3255
Fragments arranged by size:
3255 1425 1236 343 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2579 4559 4918 5077 518
Size: 648 1413 1980 359 159 2042
Fragments arranged by size:
2042 1980 1413 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2306 2531 2657 2747 2768 2963 4017 4694
Size: 700 225 126 90 21 195 1054 677
Cuts at: 4694 5842 6087 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1054 700 677 245 225 195
126 90 21
NaeI GCC'GGC
Cuts at: 3395 3395
Size: 6601
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6049
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6601
NgoMIV G'CCGG_C
Cuts at: 3393 3393
Size: 6601
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6601
NotI GC'GGCC_GC
Cuts at: 4048 4048
Size: 6601
NspI r_CATG'y
Cuts at: 1132 1229 3959 5504 1132
Size: 97 2730 1545 2229
Fragments arranged by size:
2730 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2786 1348
Size: 1438 5163
PciI A'CATG_T
Cuts at: 5500 5500
Size: 6601
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6601
PfoI T'CCnGG_A
Cuts at: 2719 2755 2870 2941 2719
Size: 36 115 71 6379
Fragments arranged by size:
6379 115 71 36
PmeI GTTT'AAAC
Cuts at: 3905 3905
Size: 6601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4670 4702 6183 6215 4670
Size: 32 1481 32 5056
Fragments arranged by size:
5056 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2591 2868 3007 3175 2591
Size: 277 139 168 6017
Fragments arranged by size:
6017 277 168 139
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6601
PsiI TTA'TAA
Cuts at: 3881 4171 3881
Size: 290 6311
PspOMI G'GGCC_C
Cuts at: 2250 2250
Size: 6601
PstI C_TGCA'G
Cuts at: 830 2461 2695 2713 2959 2971 3953 830
Size: 1631 234 18 246 12 982 3478
Fragments arranged by size:
3478 1631 982 246 234 18 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6601
PvuII CAG'CTG
Cuts at: 2306 2657 2768 2963 2306
Size: 351 111 195 5944
Fragments arranged by size:
5944 351 195 111
SalI G'TCGA_C
Cuts at: 1936 3943 1936
Size: 2007 4594
SbfI CC_TGCA'GG
Cuts at: 3953 3953
Size: 6601
ScaI AGT'ACT
Cuts at: 4849 6561 4849
Size: 1712 4889
SexAI A'CCwGG_T
Cuts at: 2864 2864
Size: 6601
SfcI C'TryA_G
Cuts at: 826 1045 2457 2691 2709 2955 2967 3766 3949
Size: 219 1412 234 18 246 12 799 183
Cuts at: 3949 5084 5765 5956 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1412 1135 799 681 246 234 219
191 183 18 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6601
SmaI CCC'GGG
Cuts at: 1872 2434 2632 3928 1872
Size: 562 198 1296 4545
Fragments arranged by size:
4545 1296 562 198
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4705 5606 5868 820
Size: 197 926 2762 901 262 1553
Fragments arranged by size:
2762 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4392 493
Size: 3899 2702
SpeI A'CTAG_T
Cuts at: 152 3886 152
Size: 3734 2867
SphI G_CATG'C
Cuts at: 1132 3959 1132
Size: 2827 3774
SspI AAT'ATT
Cuts at: 5 52 4525 5
Size: 47 4473 2081
Fragments arranged by size:
4473 2081 47
StuI AGG'CCT
Cuts at: 1517 3291 1517
Size: 1774 4827
StyI C'CwwG_G
Cuts at: 513 1065 2197 2449 2613 4040 513
Size: 552 1132 252 164 1427 3074
Fragments arranged by size:
3074 1427 1132 552 252 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2587 2600 3143 4628 4645 4798 4983
Size: 1255 1007 13 543 1485 17 153 185
Cuts at: 4983 5428 325
Size: 445 1498
Fragments arranged by size:
1498 1485 1255 1007 543 445 185 153
17 13
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3405 4847 6559
Size: 274 80 33 51 1443 1428 1442 1712
Cuts at: 6559 96
Size: 138
Fragments arranged by size:
1712 1443 1442 1428 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3020 3052 6561 6593 3020
Size: 32 3509 32 3028
Fragments arranged by size:
3509 3028 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6601
XbaI T'CTAG_A
Cuts at: 3937 3937
Size: 6601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2113 2357 2113
Size: 244 6357
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6601
XmaI C'CCGG_G
Cuts at: 1870 2432 2630 3926 1870
Size: 562 198 1296 4545
Fragments arranged by size:
4545 1296 562 198
XmnI GAAnn'nnTTC
Cuts at: 3896 4730 3896
Size: 834 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4409 276
Size: 53 83 186 936 2875 2468
Fragments arranged by size:
2875 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI