Restriction map for
pFN21AA0494
(Circular) (Six-base) MAPSORT of: pFN21AA0494.seq Check: 5237 from: 1 to: 6190
pFN21AA0494 6190 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4000 278
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3533 1937
Size: 1596 4594
Acc65I G'GTAC_C
Cuts at: 2853 3511 2853
Size: 658 5532
AclI AA'CG_TT
Cuts at: 1563 4317 4690 1563
Size: 2754 373 3063
Fragments arranged by size:
3063 2754 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2561 2733 2968 3305 3333 4253 5637 5905
Size: 929 172 235 337 28 920 1384 268
Cuts at: 5905 1632
Size: 1917
Fragments arranged by size:
1917 1384 929 920 337 268 235 172
28
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5993
AflIII A'CryG_T
Cuts at: 1203 5089 6066 1203
Size: 3886 977 1327
Fragments arranged by size:
3886 1327 977
AgeI A'CCGG_T
Cuts at: 5021 5021
Size: 6190
AhdI GACnn_n'nnGTC
Cuts at: 2128 4919 2128
Size: 2791 3399
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6190
AlwNI CAG_nnn'CTG
Cuts at: 2126 3933 5505 2126
Size: 1807 1572 2811
Fragments arranged by size:
2811 1807 1572
ApaLI G'TGCA_C
Cuts at: 4247 5403 4247
Size: 1156 5034
ApoI r'AATT_y
Cuts at: 1493 2153 2327 2788 3094 3233 3499 3725 3877
Size: 660 174 461 306 139 266 226 152
Cuts at: 3877 5986 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 660 461 306 266 226 174
152 139
AseI AT'TA_AT
Cuts at: 160 1032 4744 160
Size: 872 3712 1606
Fragments arranged by size:
3712 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
AvaI C'yCGr_G
Cuts at: 1870 1943 2879 3515 1870
Size: 73 936 636 4545
Fragments arranged by size:
4545 936 636 73
AvrII C'CTAG_G
Cuts at: 3391 3391
Size: 6190
BamHI G'GATC_C
Cuts at: 3520 3887 3520
Size: 367 5823
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2853 3511 4966 618
Size: 325 217 627 1066 658 1455 1842
Fragments arranged by size:
1842 1455 1066 658 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2025 3509 1391
Size: 634 1484 4072
Fragments arranged by size:
4072 1484 634
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2927 2961 4381 4415 1903
Size: 34 990 34 1420 34 3678
Fragments arranged by size:
3678 1420 990 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3115 4082 5292 3115
Size: 967 1210 4013
Fragments arranged by size:
4013 1210 967
BclI T'GATC_A
Cuts at: 1548 3132 1548
Size: 1584 4606
BfrBI ATG'CAT
Cuts at: 2030 2030
Size: 6190
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4801 136
Size: 107 122 71 631 605 3129 1525
Fragments arranged by size:
3129 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2214 3002 3316 6185 2214
Size: 788 314 2869 2219
Fragments arranged by size:
2869 2219 788 314
BlpI GC'TnA_GC
Cuts at: 3607 3607
Size: 6190
Bme1580I G_kGCm'C
Cuts at: 1790 2845 4251 5407 1790
Size: 1055 1406 1156 2573
Fragments arranged by size:
2573 1406 1156 1055
BmgBI CAC'GTC
Cuts at: 1318 3352 1318
Size: 2034 4156
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3251 3921 4879
Size: 416 53 83 399 24 1834 670 958
Cuts at: 4879 442
Size: 1753
Fragments arranged by size:
1834 1753 958 670 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6190
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 3140 2152
Size: 988 5202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2451 3110 3156 3485 4850 1239
Size: 1212 659 46 329 1365 2579
Fragments arranged by size:
2579 1365 1212 659 329 46
Bpu10I CC'TnA_GC
Cuts at: 3470 3470
Size: 6190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2282 2303 2435 3442 4315 5180 5478 2282
Size: 21 132 1007 873 865 298 2994
Fragments arranged by size:
2994 1007 873 865 298 132 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3170 3246 4853 882
Size: 617 1671 76 1607 2219
Fragments arranged by size:
2219 1671 1607 617 76
BsaAI yAC'GTr
Cuts at: 493 1204 3981 493
Size: 711 2777 2702
Fragments arranged by size:
2777 2702 711
BsaBI GATnn'nnATC
Cuts at: 2462 2694 6184 2462
Size: 232 3490 2468
Fragments arranged by size:
3490 2468 232
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3997 4379 5014 275
Size: 53 83 186 936 2464 382 635 1451
Fragments arranged by size:
2464 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4623 5021 5295 5442 5853 4623
Size: 398 274 147 411 4960
Fragments arranged by size:
4960 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2095 2125 3243 3273 1178
Size: 30 165 30 692 30 1118 30 4095
Fragments arranged by size:
4095 1118 692 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2843 1186
Size: 1657 4533
BseYI C'CCAG_C
Cuts at: 2045 5393 5889 2045
Size: 3348 496 2346
Fragments arranged by size:
3348 2346 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5617
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3640 4401 5429 1540
Size: 275 186 1639 761 1028 2301
Fragments arranged by size:
2301 1639 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 3142 4251 4336 5407 2025
Size: 1117 1109 85 1071 2808
Fragments arranged by size:
2808 1117 1109 1071 85
BsmI GAATG_Cn'
Cuts at: 3701 3794 3701
Size: 93 6097
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2845 3142 3509 4251 4336 5407
Size: 399 235 820 297 367 742 85 1071
Cuts at: 5407 1391
Size: 2174
Fragments arranged by size:
2174 1071 820 742 399 367 297 235
85
BspEI T'CCGG_A
Cuts at: 5853 5853
Size: 6190
BspHI T'CATG_A
Cuts at: 4079 5809 4079
Size: 1730 4460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2278 3545 844
Size: 1434 1267 3489
Fragments arranged by size:
3489 1434 1267
BsrBI CCG'CTC
Cuts at: 1394 1823 2227 4077 1394
Size: 429 404 1850 3507
Fragments arranged by size:
3507 1850 429 404
BsrDI GCAATG_nn'
Cuts at: 65 1248 3224 4685 4859 65
Size: 1183 1976 1461 174 1396
Fragments arranged by size:
1976 1461 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4834 5021 1700
Size: 110 3024 187 2869
Fragments arranged by size:
3024 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6190
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6190
BssSI C'ACGA_G
Cuts at: 4250 5262 4250
Size: 1012 5178
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6190
BstBI TT'CG_AA
Cuts at: 2332 4994 2332
Size: 2662 3528
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6190
BstYI r'GATC_y
Cuts at: 1972 2214 2305 3002 3316 3520 3887 4272 4289
Size: 242 91 697 314 204 367 385 17
Cuts at: 4289 5741 5827 5839 6185 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 697 385 367 346 314 242
204 91 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5638
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2952 487
Size: 683 56 1726 3725
Fragments arranged by size:
3725 1726 683 56
BtsI GCAGTG_nn'
Cuts at: 2511 3706 4500 4520 2511
Size: 1195 794 20 4181
Fragments arranged by size:
4181 1195 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6155
DraI TTT'AAA
Cuts at: 2610 3494 2610
Size: 884 5306
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5197 809
Size: 507 3881 1802
Fragments arranged by size:
3881 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3343 3637 4526
Size: 54 1425 50 275 18 1513 294 889
Cuts at: 4526 6155 8
Size: 1629 43
Fragments arranged by size:
1629 1513 1425 889 294 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3637 1537
Size: 275 1825 4090
Fragments arranged by size:
4090 1825 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2136 2142 2576 3183 4120 1353
Size: 783 6 434 607 937 3423
Fragments arranged by size:
3423 937 783 607 434 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4777 5151 5297 387
Size: 1064 138 3188 374 146 1280
Fragments arranged by size:
3188 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2023 2023
Size: 6190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2451 2561 2733 2968 3110 3156 3305
Size: 393 819 110 172 235 142 46 149
Cuts at: 3305 3333 3485 4253 4850 5637 5905 1239
Size: 28 152 768 597 787 268 1524
Fragments arranged by size:
1524 819 787 768 597 393 268 235
172 152 149 142 110 46 28
EcoRI G'AATT_C
Cuts at: 2153 3499 3877 2153
Size: 1346 378 4466
Fragments arranged by size:
4466 1346 378
EcoRV GAT'ATC
Cuts at: 2803 2803
Size: 6190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3042 3495 3042
Size: 453 5737
FspI TGC'GCA
Cuts at: 4696 4696
Size: 6190
HaeII r_GCGC'y
Cuts at: 1678 5337 1678
Size: 3659 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2114 2140 2146 2206
Size: 32 135 32 536 6 26 6 60
Cuts at: 2206 2238 3124 3156 4831 4863 4905 4937 1373
Size: 32 886 32 1675 32 42 32 2626
Fragments arranged by size:
2626 1675 886 536 135 60 42 32
32 32 32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2496 2923 3534 3780 669
Size: 988 281 558 427 611 246 3079
Fragments arranged by size:
3079 988 611 558 427 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6190
HpaI GTT'AAC
Cuts at: 3780 3780
Size: 6190
KpnI G_GTAC'C
Cuts at: 2857 3515 2857
Size: 658 5532
MfeI C'AATT_G
Cuts at: 3789 3789
Size: 6190
MluI A'CGCG_T
Cuts at: 6066 6066
Size: 6190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2146 5304 5488 1305
Size: 841 3158 184 2007
Fragments arranged by size:
3158 2007 841 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3345 10
Size: 54 1425 343 1513 2855
Fragments arranged by size:
2855 1513 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2471 4148 4507 4666 518
Size: 648 1305 1677 359 159 2042
Fragments arranged by size:
2042 1677 1305 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2537 3130 3606 4283 5431 5676 1606
Size: 931 593 476 677 1148 245 2120
Fragments arranged by size:
2120 1148 931 677 593 476 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5638
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6190
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6190
NotI GC'GGCC_GC
Cuts at: 3637 3637
Size: 6190
NsiI A_TGCA'T
Cuts at: 2032 2032
Size: 6190
NspI r_CATG'y
Cuts at: 1132 1229 3548 5093 1132
Size: 97 2319 1545 2229
Fragments arranged by size:
2319 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6190
PciI A'CATG_T
Cuts at: 5089 5089
Size: 6190
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3352 1108
Size: 2244 3946
PfoI T'CCnGG_A
Cuts at: 2719 3462 2719
Size: 743 5447
PmeI GTTT'AAAC
Cuts at: 3494 3494
Size: 6190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 5772 5804 4259
Size: 32 1481 32 4645
Fragments arranged by size:
4645 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6190
PsiI TTA'TAA
Cuts at: 3760 3760
Size: 6190
PspXI vC'TCGA_Gb
Cuts at: 2879 2879
Size: 6190
PstI C_TGCA'G
Cuts at: 830 2506 2542 2685 3542 830
Size: 1676 36 143 857 3478
Fragments arranged by size:
3478 1676 857 143 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6190
PvuII CAG'CTG
Cuts at: 2537 3130 2537
Size: 593 5597
SacI G_AGCT'C
Cuts at: 2025 2025
Size: 6190
SalI G'TCGA_C
Cuts at: 1936 3532 1936
Size: 1596 4594
SapI GCTCTTCn'nnn_
Cuts at: 3183 3183
Size: 6190
SbfI CC_TGCA'GG
Cuts at: 3542 3542
Size: 6190
ScaI AGT'ACT
Cuts at: 4438 6150 4438
Size: 1712 4478
SfcI C'TryA_G
Cuts at: 826 1045 2502 2538 2565 2639 2681 3538 4673
Size: 219 1457 36 27 74 42 857 1135
Cuts at: 4673 5354 5545 826
Size: 681 191 1471
Fragments arranged by size:
1471 1457 1135 857 681 219 191 74
42 36 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6190
SmaI CCC'GGG
Cuts at: 1872 3517 1872
Size: 1645 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2297 2318 2414 2879 3421 4294
Size: 197 926 354 21 96 465 542 873
Cuts at: 4294 5195 5457 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 873 542 465 354 262
197 96 21
SnaBI TAC'GTA
Cuts at: 493 3981 493
Size: 3488 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6190
SphI G_CATG'C
Cuts at: 1132 3548 1132
Size: 2416 3774
SspI AAT'ATT
Cuts at: 5 52 4114 5
Size: 47 4062 2081
Fragments arranged by size:
4062 2081 47
StuI AGG'CCT
Cuts at: 1517 3169 1517
Size: 1652 4538
StyI C'CwwG_G
Cuts at: 513 1065 2187 3391 3629 513
Size: 552 1122 1204 238 3074
Fragments arranged by size:
3074 1204 1122 552 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2984 4217 4234 4387 4572 5017 325
Size: 1255 1404 1233 17 153 185 445 1498
Fragments arranged by size:
1498 1404 1255 1233 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4436 6148 96
Size: 274 80 33 51 1443 2459 1712 138
Fragments arranged by size:
2459 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2095 2127 6150 6182 2095
Size: 32 4023 32 2103
Fragments arranged by size:
4023 2103 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6190
XbaI T'CTAG_A
Cuts at: 3526 3526
Size: 6190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3353 3353
Size: 6190
XhoI C'TCGA_G
Cuts at: 1943 2879 1943
Size: 936 5254
XmaI C'CCGG_G
Cuts at: 1870 3515 1870
Size: 1645 4545
XmnI GAAnn'nnTTC
Cuts at: 2328 3318 4319 2328
Size: 990 1001 4199
Fragments arranged by size:
4199 1001 990
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3998 276
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI