Restriction map for
pFN21AA0481
(Circular) (Six-base) MAPSORT of: pFN21AA0481.seq Check: 8965 from: 1 to: 6832
pFN21AA0481 6832 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2094 2601 2886 2977 3874 2094
Size: 507 285 91 897 5052
Fragments arranged by size:
5052 897 507 285 91
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2309 4642 278
Size: 53 83 186 936 773 2333 2468
Fragments arranged by size:
2468 2333 936 773 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4175 1937
Size: 2238 4594
Acc65I G'GTAC_C
Cuts at: 4153 4153
Size: 6832
AclI AA'CG_TT
Cuts at: 1563 4959 5332 1563
Size: 3396 373 3063
Fragments arranged by size:
3396 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2133 2276 2915 3107 3176 3359 3644 3773
Size: 501 143 639 192 69 183 285 129
Cuts at: 3773 4895 6279 6547 1632
Size: 1122 1384 268 1917
Fragments arranged by size:
1917 1384 1122 639 501 285 268 192
183 143 129 69
AfeI AGC'GCT
Cuts at: 2378 3680 2378
Size: 1302 5530
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6635
AflIII A'CryG_T
Cuts at: 1203 2709 4116 5731 6708 1203
Size: 1506 1407 1615 977 1327
Fragments arranged by size:
1615 1506 1407 1327 977
AgeI A'CCGG_T
Cuts at: 5663 5663
Size: 6832
AhdI GACnn_n'nnGTC
Cuts at: 5561 5561
Size: 6832
AleI CACnn'nnGTG
Cuts at: 1166 3263 1166
Size: 2097 4735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6832
AlwNI CAG_nnn'CTG
Cuts at: 2224 3154 3667 3763 4575 6147 2224
Size: 930 513 96 812 1572 2909
Fragments arranged by size:
2909 1572 930 812 513 96
ApaI G_GGCC'C
Cuts at: 3111 3363 3111
Size: 252 6580
ApaLI G'TGCA_C
Cuts at: 4889 6045 4889
Size: 1156 5676
ApoI r'AATT_y
Cuts at: 1493 4141 4367 4519 6628 1493
Size: 2648 226 152 2109 1697
Fragments arranged by size:
2648 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5386 160
Size: 872 4354 1606
Fragments arranged by size:
4354 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6832
AvaI C'yCGr_G
Cuts at: 1870 1943 2286 3279 3378 3846 4157 1870
Size: 73 343 993 99 468 311 4545
Fragments arranged by size:
4545 993 468 343 311 99 73
BamHI G'GATC_C
Cuts at: 4162 4529 4162
Size: 367 6465
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2170 2495 2839 3081 3167
Size: 325 217 627 383 325 344 242 86
Cuts at: 3167 4153 5608 618
Size: 986 1455 1842
Fragments arranged by size:
1842 1455 986 627 383 344 325 325
242 217 86
BanII G_rGCy'C
Cuts at: 1391 2237 2574 3111 3363 3515 4151 1391
Size: 846 337 537 252 152 636 4072
Fragments arranged by size:
4072 846 636 537 337 252 152
BbeI G_GCGC'C
Cuts at: 2174 2499 3085 3171 2174
Size: 325 586 86 5835
Fragments arranged by size:
5835 586 325 86
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3624 3632 4028 928
Size: 971 1725 8 396 3732
Fragments arranged by size:
3732 1725 971 396 8
BbvCI CC'TCA_GC
Cuts at: 2724 2724
Size: 6832
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2912 2946 5023 5057 1903
Size: 34 975 34 2077 34 3678
Fragments arranged by size:
3678 2077 975 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4724 5934 4724
Size: 1210 5622
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6832
BfrBI ATG'CAT
Cuts at: 2392 2392
Size: 6832
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2434 3115 3237
Size: 107 122 71 631 605 762 681 122
Cuts at: 3237 5443 136
Size: 2206 1525
Fragments arranged by size:
2206 1525 762 681 631 605 122 122
107 71
BglII A'GATC_T
Cuts at: 6827 6827
Size: 6832
BlpI GC'TnA_GC
Cuts at: 3460 4249 3460
Size: 789 6043
Bme1580I G_kGCm'C
Cuts at: 1790 2832 2842 3111 3363 3385 3811 4893 6049
Size: 1042 10 269 252 22 426 1082 1156
Cuts at: 6049 1790
Size: 2573
Fragments arranged by size:
2573 1156 1082 1042 426 269 252 22
10
BmgBI CAC'GTC
Cuts at: 1318 2827 3130 3163 1318
Size: 1509 303 33 4987
Fragments arranged by size:
4987 1509 303 33
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3655 4096 4563
Size: 416 53 83 399 24 2238 441 467
Cuts at: 4563 5521 442
Size: 958 1753
Fragments arranged by size:
2238 1753 958 467 441 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6832
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2588 3086 3394 3427 3623 3719 3881 3917
Size: 1349 498 308 33 196 96 162 36
Cuts at: 3917 4130 5492 1239
Size: 213 1362 2579
Fragments arranged by size:
2579 1362 1349 498 308 213 196 162
96 36 33
Bpu10I CC'TnA_GC
Cuts at: 2724 2777 2724
Size: 53 6779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4957 5822 6120 4957
Size: 865 298 5669
Fragments arranged by size:
5669 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2108 2289 2757 5495 882
Size: 617 609 181 468 2738 2219
Fragments arranged by size:
2738 2219 617 609 468 181
BsaAI yAC'GTr
Cuts at: 493 1204 4117 4623 493
Size: 711 2913 506 2702
Fragments arranged by size:
2913 2702 711 506
BsaBI GATnn'nnATC
Cuts at: 6826 6826
Size: 6832
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2154 2171 2306 2496
Size: 53 83 186 936 621 17 135 190
Cuts at: 2496 3082 3168 3219 4639 5021 5656 275
Size: 586 86 51 1420 382 635 1451
Fragments arranged by size:
1451 1420 936 635 621 586 382 190
186 135 86 83 53 51 17
BsaWI w'CCGG_w
Cuts at: 2383 2842 5265 5663 5937 6084 6495 2383
Size: 459 2423 398 274 147 411 2720
Fragments arranged by size:
2720 2423 459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2342 2372 2754 2784 3578
Size: 30 165 30 939 30 382 30 794
Cuts at: 3578 3608 4076 4103 4106 4133 1178
Size: 30 468 27 3 27 3877
Fragments arranged by size:
3877 939 794 468 382 165 30 30
30 30 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2741 3383 3689 3716 3791 4064 1186
Size: 877 678 642 306 27 75 273 3954
Fragments arranged by size:
3954 877 678 642 306 273 75 27
BseYI C'CCAG_C
Cuts at: 2060 2319 2574 2850 3048 3488 3505 3931 4127
Size: 259 255 276 198 440 17 426 196
Cuts at: 4127 6035 6531 2060
Size: 1908 496 2361
Fragments arranged by size:
2361 1908 496 440 426 276 259 255
198 196 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2035 2402 2489 2621 2864 3038 1163
Size: 573 299 367 87 132 243 174 4957
Fragments arranged by size:
4957 573 367 299 243 174 132 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4282 5043 6071 1540
Size: 275 186 2281 761 1028 2301
Fragments arranged by size:
2301 2281 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2301 3072 3443 3541 4116 4893 4978 6049 2301
Size: 771 371 98 575 777 85 1071 3084
Fragments arranged by size:
3084 1071 777 771 575 371 98 85
BsmI GAATG_Cn'
Cuts at: 3916 4343 4436 3916
Size: 427 93 6312
Fragments arranged by size:
6312 427 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2313 2404 3412 2313
Size: 91 1008 5733
Fragments arranged by size:
5733 1008 91
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2301 2574 2832 2842 3072 3111
Size: 399 447 64 273 258 10 230 39
Cuts at: 3111 3363 3385 3443 3515 3541 3811 4116 4151
Size: 252 22 58 72 26 270 305 35
Cuts at: 4151 4893 4978 6049 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 447 399 305 273 270
258 252 230 85 72 64 58 39
35 26 22 10
BspEI T'CCGG_A
Cuts at: 2383 6495 2383
Size: 4112 2720
BspHI T'CATG_A
Cuts at: 4017 4721 6451 4017
Size: 704 1730 4398
Fragments arranged by size:
4398 1730 704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2094 2433 2601 2886 2977 3874 4187 844
Size: 1250 339 168 285 91 897 313 3489
Fragments arranged by size:
3489 1250 897 339 313 285 168 91
BsrBI CCG'CTC
Cuts at: 1394 1823 2472 2661 3390 3694 4719 1394
Size: 429 649 189 729 304 1025 3507
Fragments arranged by size:
3507 1025 729 649 429 304 189
BsrDI GCAATG_nn'
Cuts at: 65 1248 5327 5501 65
Size: 1183 4079 174 1396
Fragments arranged by size:
4079 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2156 2544 2789 3078 5476 5663 1700
Size: 110 346 388 245 289 2398 187 2869
Fragments arranged by size:
2869 2398 388 346 289 245 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6832
BssHII G'CGCG_C
Cuts at: 1925 3178 1925
Size: 1253 5579
BssSI C'ACGA_G
Cuts at: 4892 5904 4892
Size: 1012 5820
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2425 4120 794
Size: 1631 1695 3506
Fragments arranged by size:
3506 1695 1631
BstBI TT'CG_AA
Cuts at: 5636 5636
Size: 6832
BstEII G'GTnAC_C
Cuts at: 1184 3850 1184
Size: 2666 4166
BstXI CCAn_nnnn'nTGG
Cuts at: 2821 4054 2821
Size: 1233 5599
BstYI r'GATC_y
Cuts at: 1972 2466 2889 4162 4529 4914 4931 6383 6469
Size: 494 423 1273 367 385 17 1452 86
Cuts at: 6469 6481 6827 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1273 494 423 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3366 3366
Size: 6832
BtgI C'CryG_G
Cuts at: 513 1065 2118 2166 2248 2794 3264 3612 3828
Size: 552 1053 48 82 546 470 348 216
Cuts at: 3828 513
Size: 3517
Fragments arranged by size:
3517 1053 552 546 470 348 216 82
48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2378 3028 3313 3444 3979 487
Size: 683 56 1152 650 285 131 535 3340
Fragments arranged by size:
3340 1152 683 650 535 285 131 56
BtsI GCAGTG_nn'
Cuts at: 2521 2679 3322 3400 4348 5142 5162 2521
Size: 158 643 78 948 794 20 4191
Fragments arranged by size:
4191 948 794 643 158 78 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6832
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6797
DraI TTT'AAA
Cuts at: 2208 4136 2208
Size: 1928 4904
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5839 809
Size: 507 4523 1802
Fragments arranged by size:
4523 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2245 2625 2694
Size: 54 1425 50 275 18 415 380 69
Cuts at: 2694 2791 2964 3765 3966 4279 5168 6797 8
Size: 97 173 801 201 313 889 1629 43
Fragments arranged by size:
1629 1425 889 801 415 380 313 275
201 173 97 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4279 1537
Size: 275 2467 4090
Fragments arranged by size:
4090 2467 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2003 2668 3561 4762 1353
Size: 650 665 893 1201 3423
Fragments arranged by size:
3423 1201 893 665 650
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5419 5793 5939 387
Size: 1064 138 3830 374 146 1280
Fragments arranged by size:
3830 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2133 2276 2588 2915 3086 3107 3176
Size: 393 501 143 312 327 171 21 69
Cuts at: 3176 3359 3394 3427 3623 3644 3719 3773 3881
Size: 183 35 33 196 21 75 54 108
Cuts at: 3881 3917 4130 4895 5492 6279 6547 1239
Size: 36 213 765 597 787 268 1524
Fragments arranged by size:
1524 787 765 597 501 393 327 312
268 213 196 183 171 143 108 75
69 54 36 35 33 21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2723 3245 3671 3755 3802 2723
Size: 522 426 84 47 5753
Fragments arranged by size:
5753 522 426 84 47
EcoO109I rG'GnC_Cy
Cuts at: 2183 2605 3108 3343 3360 2183
Size: 422 503 235 17 5655
Fragments arranged by size:
5655 503 422 235 17
EcoRI G'AATT_C
Cuts at: 4141 4519 4141
Size: 378 6454
FspI TGC'GCA
Cuts at: 2600 4034 5338 2600
Size: 1434 1304 4094
Fragments arranged by size:
4094 1434 1304
HaeII r_GCGC'y
Cuts at: 1678 2163 2174 2380 2499 3085 3171 3450 3456
Size: 485 11 206 119 586 86 279 6
Cuts at: 3456 3506 3682 3929 3962 5979 1678
Size: 50 176 247 33 2017 2531
Fragments arranged by size:
2531 2017 586 485 279 247 206 176
119 86 50 33 11 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2342 2374 3425 3457 3578
Size: 32 135 32 770 32 1051 32 121
Cuts at: 3578 3610 5473 5505 5547 5579 1373
Size: 32 1863 32 42 32 2626
Fragments arranged by size:
2626 1863 1051 770 135 121 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4176 4422 669
Size: 988 281 2238 246 3079
Fragments arranged by size:
3079 2238 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6832
HpaI GTT'AAC
Cuts at: 4422 4422
Size: 6832
KasI G'GCGC_C
Cuts at: 2170 2495 3081 3167 2170
Size: 325 586 86 5835
Fragments arranged by size:
5835 586 325 86
KpnI G_GTAC'C
Cuts at: 4157 4157
Size: 6832
MfeI C'AATT_G
Cuts at: 4431 4431
Size: 6832
MluI A'CGCG_T
Cuts at: 6708 6708
Size: 6832
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2373 5946 6130 1305
Size: 1068 3573 184 2007
Fragments arranged by size:
3573 2007 1068 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2247 2966 3767 10
Size: 54 1425 343 415 719 801 3075
Fragments arranged by size:
3075 1425 801 719 415 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3263 3653 3947 3968 4790 5149 5308
Size: 648 2097 390 294 21 822 359 159
Cuts at: 5308 518
Size: 2042
Fragments arranged by size:
2097 2042 822 648 390 359 294 159
21
MspA1I CmG'CkG
Cuts at: 1606 2168 2224 2532 2854 3052 3192 3634 3895
Size: 562 56 308 322 198 140 442 261
Cuts at: 3895 4248 4925 6073 6318 1606
Size: 353 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 562 442 353 322 308
261 245 198 140 56
NaeI GCC'GGC
Cuts at: 2158 2546 2791 3080 2158
Size: 388 245 289 5910
Fragments arranged by size:
5910 388 289 245
NarI GG'CG_CC
Cuts at: 2171 2496 3082 3168 2171
Size: 325 586 86 5835
Fragments arranged by size:
5835 586 325 86
NcoI C'CATG_G
Cuts at: 513 1065 2794 3612 513
Size: 552 1729 818 3733
Fragments arranged by size:
3733 1729 818 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6832
NgoMIV G'CCGG_C
Cuts at: 2156 2544 2789 3078 2156
Size: 388 245 289 5910
Fragments arranged by size:
5910 388 289 245
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6832
NotI GC'GGCC_GC
Cuts at: 4279 4279
Size: 6832
NruI TCG'CGA
Cuts at: 2934 2934
Size: 6832
NsiI A_TGCA'T
Cuts at: 2394 2394
Size: 6832
NspI r_CATG'y
Cuts at: 1132 1229 3145 4190 5735 1132
Size: 97 1916 1045 1545 2229
Fragments arranged by size:
2229 1916 1545 1045 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6832
PciI A'CATG_T
Cuts at: 5731 5731
Size: 6832
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6832
PfoI T'CCnGG_A
Cuts at: 3541 3819 4107 3541
Size: 278 288 6266
Fragments arranged by size:
6266 288 278
PmeI GTTT'AAAC
Cuts at: 4136 4136
Size: 6832
PmlI CAC'GTG
Cuts at: 4117 4117
Size: 6832
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3095 3127 4901 4933 6414 6446 3095
Size: 32 1774 32 1481 32 3481
Fragments arranged by size:
3481 1774 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2183 3343 2183
Size: 1160 5672
PshAI GACnn'nnGTC
Cuts at: 1613 3848 1613
Size: 2235 4597
PsiI TTA'TAA
Cuts at: 4402 4402
Size: 6832
PspOMI G'GGCC_C
Cuts at: 3107 3359 3107
Size: 252 6580
PstI C_TGCA'G
Cuts at: 830 2034 2463 3639 3672 3870 4184 830
Size: 1204 429 1176 33 198 314 3478
Fragments arranged by size:
3478 1204 1176 429 314 198 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6832
PvuII CAG'CTG
Cuts at: 2224 3052 3634 3895 2224
Size: 828 582 261 5161
Fragments arranged by size:
5161 828 582 261
SacII CC_GC'GG
Cuts at: 2169 2169
Size: 6832
SalI G'TCGA_C
Cuts at: 1936 4174 1936
Size: 2238 4594
SapI GCTCTTCn'nnn_
Cuts at: 2668 3561 2668
Size: 893 5939
SbfI CC_TGCA'GG
Cuts at: 3672 4184 3672
Size: 512 6320
ScaI AGT'ACT
Cuts at: 5080 6792 5080
Size: 1712 5120
SexAI A'CCwGG_T
Cuts at: 2766 2766
Size: 6832
SfcI C'TryA_G
Cuts at: 826 1045 2030 2459 3635 3668 3682 3866 4180
Size: 219 985 429 1176 33 14 184 314
Cuts at: 4180 5315 5996 6187 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1176 1135 985 681 429 314 219
191 184 33 14
SfoI GGC'GCC
Cuts at: 2172 2497 3083 3169 2172
Size: 325 586 86 5835
Fragments arranged by size:
5835 586 325 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6832
SgrAI Cr'CCGG_yG
Cuts at: 1700 2156 3078 1700
Size: 456 922 5454
Fragments arranged by size:
5454 922 456
SmaI CCC'GGG
Cuts at: 1872 3281 3380 3848 4159 1872
Size: 1409 99 468 311 4545
Fragments arranged by size:
4545 1409 468 311 99
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4936 5837 6099 820
Size: 197 926 2993 901 262 1553
Fragments arranged by size:
2993 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4623 493
Size: 4130 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6832
SphI G_CATG'C
Cuts at: 1132 4190 1132
Size: 3058 3774
SrfI GCCC'GGGC
Cuts at: 3380 3380
Size: 6832
SspI AAT'ATT
Cuts at: 5 52 4756 5
Size: 47 4704 2081
Fragments arranged by size:
4704 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6832
StyI C'CwwG_G
Cuts at: 513 1065 2794 2859 3123 3612 4271 513
Size: 552 1729 65 264 489 659 3074
Fragments arranged by size:
3074 1729 659 552 489 264 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2271 3269 4859 4876 5029 5214 5659
Size: 1255 691 998 1590 17 153 185 445
Cuts at: 5659 325
Size: 1498
Fragments arranged by size:
1590 1498 1255 998 691 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5078 6790 96
Size: 274 80 33 51 1443 3101 1712 138
Fragments arranged by size:
3101 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2754 2786 3801 3833 4076 4108 6792 6824 2754
Size: 32 1015 32 243 32 2684 32 2762
Fragments arranged by size:
2762 2684 1015 243 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2750 1316
Size: 1434 5398
XbaI T'CTAG_A
Cuts at: 4168 4168
Size: 6832
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6832
XmaI C'CCGG_G
Cuts at: 1870 3279 3378 3846 4157 1870
Size: 1409 99 468 311 4545
Fragments arranged by size:
4545 1409 468 311 99
XmnI GAAnn'nnTTC
Cuts at: 4961 4961
Size: 6832
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2307 4640 276
Size: 53 83 186 936 773 2333 2468
Fragments arranged by size:
2468 2333 936 773 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BplI BsiWI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI PacI PspXI PsrI
RsrII SacI SanDI SfiI SwaI XcmI