Restriction map for
pFN21AA0472
(Circular) (Six-base) MAPSORT of: pFN21AA0472.seq Check: 6001 from: 1 to: 7492
pFN21AA0472 7492 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2398 3685 2398
Size: 1287 6205
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3333 5302 278
Size: 53 83 186 936 1797 1969 2468
Fragments arranged by size:
2468 1969 1797 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4002 4497 4835 1937
Size: 2065 495 338 4594
Fragments arranged by size:
4594 2065 495 338
Acc65I G'GTAC_C
Cuts at: 4813 4813
Size: 7492
AclI AA'CG_TT
Cuts at: 1563 5619 5992 1563
Size: 4056 373 3063
Fragments arranged by size:
4056 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2783 4259 5555 6939 7207 1632
Size: 1151 1476 1296 1384 268 1917
Fragments arranged by size:
1917 1476 1384 1296 1151 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7295
AflIII A'CryG_T
Cuts at: 1203 4262 6391 7368 1203
Size: 3059 2129 977 1327
Fragments arranged by size:
3059 2129 1327 977
AgeI A'CCGG_T
Cuts at: 6323 6323
Size: 7492
AhdI GACnn_n'nnGTC
Cuts at: 6221 6221
Size: 7492
AleI CACnn'nnGTG
Cuts at: 1166 3665 1166
Size: 2499 4993
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4208 4389 1184
Size: 3024 181 4287
Fragments arranged by size:
4287 3024 181
AlwNI CAG_nnn'CTG
Cuts at: 2137 2955 3508 3976 4538 5235 6807 2137
Size: 818 553 468 562 697 1572 2822
Fragments arranged by size:
2822 1572 818 697 562 553 468
ApaLI G'TGCA_C
Cuts at: 2525 3098 5549 6705 2525
Size: 573 2451 1156 3312
Fragments arranged by size:
3312 2451 1156 573
ApoI r'AATT_y
Cuts at: 1493 4094 4509 4801 5027 5179 7288 1493
Size: 2601 415 292 226 152 2109 1697
Fragments arranged by size:
2601 2109 1697 415 292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6046 160
Size: 872 5014 1606
Fragments arranged by size:
5014 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7492
AvaI C'yCGr_G
Cuts at: 1870 1943 2044 2487 4817 1870
Size: 73 101 443 2330 4545
Fragments arranged by size:
4545 2330 443 101 73
BamHI G'GATC_C
Cuts at: 4822 5189 4822
Size: 367 7125
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2457 3362 4813 6268
Size: 325 217 627 233 437 905 1451 1455
Cuts at: 6268 618
Size: 1842
Fragments arranged by size:
1842 1455 1451 905 627 437 325 233
217
BanII G_rGCy'C
Cuts at: 1391 2040 2861 2971 3674 4612 4811 1391
Size: 649 821 110 703 938 199 4072
Fragments arranged by size:
4072 938 821 703 649 199 110
BbeI G_GCGC'C
Cuts at: 2461 2461
Size: 7492
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4616 928
Size: 971 2717 3804
Fragments arranged by size:
3804 2717 971
BbvCI CC'TCA_GC
Cuts at: 3731 3872 3731
Size: 141 7351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3785 3819 5683 5717 1903
Size: 34 1848 34 1864 34 3678
Fragments arranged by size:
3678 1864 1848 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2974 5384 6594 2974
Size: 2410 1210 3872
Fragments arranged by size:
3872 2410 1210
BclI T'GATC_A
Cuts at: 1548 3444 1548
Size: 1896 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2061 2238 3840
Size: 107 122 71 631 605 389 177 1602
Cuts at: 3840 6103 136
Size: 2263 1525
Fragments arranged by size:
2263 1602 1525 631 605 389 177 122
107 71
BglII A'GATC_T
Cuts at: 3561 3862 7487 3561
Size: 301 3625 3566
Fragments arranged by size:
3625 3566 301
BlpI GC'TnA_GC
Cuts at: 4909 4909
Size: 7492
Bme1580I G_kGCm'C
Cuts at: 1790 2529 3102 4042 5553 6709 1790
Size: 739 573 940 1511 1156 2573
Fragments arranged by size:
2573 1511 1156 940 739 573
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7492
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2563 2864 3974
Size: 416 53 83 399 24 1146 301 1110
Cuts at: 3974 3986 4027 5223 6181 442
Size: 12 41 1196 958 1753
Fragments arranged by size:
1753 1196 1146 1110 958 416 399 301
83 53 41 24 12
BmtI G_CTAG'C
Cuts at: 1056 4575 1056
Size: 3519 3973
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2059 4626 2059
Size: 2567 4925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2600 2648 3530 4044 4165 4277 4706
Size: 1061 300 48 882 514 121 112 429
Cuts at: 4706 6152 1239
Size: 1446 2579
Fragments arranged by size:
2579 1446 1061 882 514 429 300 121
112 48
Bpu10I CC'TnA_GC
Cuts at: 3731 3872 4531 3731
Size: 141 659 6692
Fragments arranged by size:
6692 659 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3052 5617 6482 6780 3052
Size: 2565 865 298 3764
Fragments arranged by size:
3764 2565 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2138 2552 4116 6155 882
Size: 617 639 414 1564 2039 2219
Fragments arranged by size:
2219 2039 1564 639 617 414
BsaAI yAC'GTr
Cuts at: 493 1204 5283 493
Size: 711 4079 2702
Fragments arranged by size:
4079 2702 711
BsaBI GATnn'nnATC
Cuts at: 7486 7486
Size: 7492
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2458 3330 5299 5681
Size: 53 83 186 936 925 872 1969 382
Cuts at: 5681 6316 275
Size: 635 1451
Fragments arranged by size:
1969 1451 936 925 872 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3771 5925 6323 6597 6744 7155 3771
Size: 2154 398 274 147 411 4108
Fragments arranged by size:
4108 2154 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3188 3218 3342 3372 1178
Size: 30 165 30 1785 30 124 30 5298
Fragments arranged by size:
5298 1785 165 124 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2188 2453 2585 2866 4187 4655 1186
Size: 1002 265 132 281 1321 468 4023
Fragments arranged by size:
4023 1321 1002 468 281 265 132
BseYI C'CCAG_C
Cuts at: 2428 4244 6695 7191 2428
Size: 1816 2451 496 2729
Fragments arranged by size:
2729 2451 1816 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2426 4292 1163
Size: 573 690 1866 4363
Fragments arranged by size:
4363 1866 690 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2101 4942 5703 6731 1540
Size: 275 186 100 2841 761 1028 2301
Fragments arranged by size:
2841 2301 1028 761 275 186 100
BsiHKAI G_wGCw'C
Cuts at: 2177 2529 3038 3102 4197 5553 5638 6709 2177
Size: 352 509 64 1095 1356 85 1071 2960
Fragments arranged by size:
2960 1356 1095 1071 509 352 85 64
BsmI GAATG_Cn'
Cuts at: 5003 5096 5003
Size: 93 7399
BsmBI CGTCTCn'nnnn_
Cuts at: 2047 2047
Size: 7492
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2177 2529 2861 2971 3038 3102
Size: 399 250 137 352 332 110 67 64
Cuts at: 3102 3674 4042 4197 4612 4811 5553 5638 6709
Size: 572 368 155 415 199 742 85 1071
Cuts at: 6709 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 572 415 399 368 352
332 250 199 155 137 110 85 67
64
BspEI T'CCGG_A
Cuts at: 3771 7155 3771
Size: 3384 4108
BspHI T'CATG_A
Cuts at: 4731 5381 7111 4731
Size: 650 1730 5112
Fragments arranged by size:
5112 1730 650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2145 2398 3162 3685 4847 844
Size: 1301 253 764 523 1162 3489
Fragments arranged by size:
3489 1301 1162 764 523 253
BsrBI CCG'CTC
Cuts at: 1394 1823 4210 5379 1394
Size: 429 2387 1169 3507
Fragments arranged by size:
3507 2387 1169 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4185 5987 6161 65
Size: 1183 2937 1802 174 1396
Fragments arranged by size:
2937 1802 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3843 6136 6323 1700
Size: 110 2033 2293 187 2869
Fragments arranged by size:
2869 2293 2033 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7492
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7492
BssSI C'ACGA_G
Cuts at: 3794 5552 6564 3794
Size: 1758 1012 4722
Fragments arranged by size:
4722 1758 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7492
BstBI TT'CG_AA
Cuts at: 6296 6296
Size: 7492
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7492
BstXI CCAn_nnnn'nTGG
Cuts at: 2428 2890 3175 3688 2428
Size: 462 285 513 6232
Fragments arranged by size:
6232 513 462 285
BstYI r'GATC_y
Cuts at: 1972 2575 3393 3561 3862 4138 4222 4822 5189
Size: 603 818 168 301 276 84 600 367
Cuts at: 5189 5574 5591 7043 7129 7141 7487 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 818 603 600 385 367 346
301 276 168 86 84 17 12
BstZ17I GTA'TAC
Cuts at: 4003 4003
Size: 7492
Bsu36I CC'TnA_GG
Cuts at: 2298 2568 3464 4551 2298
Size: 270 896 1087 5239
Fragments arranged by size:
5239 1087 896 270
BtgI C'CryG_G
Cuts at: 513 1065 2004 2024 4144 4488 513
Size: 552 939 20 2120 344 3517
Fragments arranged by size:
3517 2120 939 552 344 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2256 487
Size: 683 56 1030 5723
Fragments arranged by size:
5723 1030 683 56
BtsI GCAGTG_nn'
Cuts at: 2440 3100 3106 3435 5008 5802 5822 2440
Size: 660 6 329 1573 794 20 4110
Fragments arranged by size:
4110 1573 794 660 329 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7492
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4153 4188 529
Size: 35 3589 35 3833
Fragments arranged by size:
3833 3589 35 35
DraI TTT'AAA
Cuts at: 3325 4796 3325
Size: 1471 6021
DraIII CAC_nnn'GTG
Cuts at: 3052 3052
Size: 7492
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3335 6499 809
Size: 507 2019 3164 1802
Fragments arranged by size:
3164 2019 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2356 2974
Size: 54 1425 50 275 18 268 258 618
Cuts at: 2974 3832 4939 5828 7457 8
Size: 858 1107 889 1629 43
Fragments arranged by size:
1629 1425 1107 889 858 618 275 268
258 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2098 4939 1537
Size: 275 286 2841 4090
Fragments arranged by size:
4090 2841 286 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2298 3692 4682 5422 1353
Size: 945 1394 990 740 3423
Fragments arranged by size:
3423 1394 990 945 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2082 6079 6453 6599 387
Size: 1064 138 493 3997 374 146 1280
Fragments arranged by size:
3997 1280 1064 493 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2600 2648 2783 3530 4044 4165
Size: 393 668 300 48 135 747 514 121
Cuts at: 4165 4259 4277 4706 5555 6152 6939 7207 1239
Size: 94 18 429 849 597 787 268 1524
Fragments arranged by size:
1524 849 787 747 668 597 514 429
393 300 268 135 121 94 48 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2357 2881 4687 2357
Size: 524 1806 5162
Fragments arranged by size:
5162 1806 524
EcoO109I rG'GnC_Cy
Cuts at: 2048 3085 3151 2048
Size: 1037 66 6389
Fragments arranged by size:
6389 1037 66
EcoRI G'AATT_C
Cuts at: 4509 4801 5179 4509
Size: 292 378 6822
Fragments arranged by size:
6822 378 292
EcoRV GAT'ATC
Cuts at: 4336 4336
Size: 7492
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3945 3945
Size: 7492
FspI TGC'GCA
Cuts at: 5998 5998
Size: 7492
HaeII r_GCGC'y
Cuts at: 1678 2461 2504 3738 6639 1678
Size: 783 43 1234 2901 2531
Fragments arranged by size:
2901 2531 1234 783 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2470 2502 2759 2791 2935
Size: 32 135 32 898 32 257 32 144
Cuts at: 2935 2967 6133 6165 6207 6239 1373
Size: 32 3166 32 42 32 2626
Fragments arranged by size:
3166 2626 898 257 144 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4836 5082 669
Size: 988 281 2898 246 3079
Fragments arranged by size:
3079 2898 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2450 3472 748
Size: 1702 1022 4768
Fragments arranged by size:
4768 1702 1022
HpaI GTT'AAC
Cuts at: 5082 5082
Size: 7492
KasI G'GCGC_C
Cuts at: 2457 2457
Size: 7492
KpnI G_GTAC'C
Cuts at: 4817 4817
Size: 7492
MfeI C'AATT_G
Cuts at: 5091 5091
Size: 7492
MluI A'CGCG_T
Cuts at: 7368 7368
Size: 7492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3412 3460 6606 6790 1305
Size: 2107 48 3146 184 2007
Fragments arranged by size:
3146 2107 2007 184 48
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2976 3834 10
Size: 54 1425 343 1144 858 3668
Fragments arranged by size:
3668 1425 1144 858 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2766 3002 3665 4077 4190 5450 5809
Size: 648 1600 236 663 412 113 1260 359
Cuts at: 5809 5968 518
Size: 159 2042
Fragments arranged by size:
2042 1600 1260 663 648 412 359 236
159 113
MspA1I CmG'CkG
Cuts at: 1606 2383 3148 3823 4908 5585 6733 6978 1606
Size: 777 765 675 1085 677 1148 245 2120
Fragments arranged by size:
2120 1148 1085 777 765 677 675 245
NarI GG'CG_CC
Cuts at: 2458 2458
Size: 7492
NcoI C'CATG_G
Cuts at: 513 1065 2004 2024 4144 513
Size: 552 939 20 2120 3861
Fragments arranged by size:
3861 2120 939 552 20
NdeI CA'TA_TG
Cuts at: 387 4449 387
Size: 4062 3430
NheI G'CTAG_C
Cuts at: 1052 4571 1052
Size: 3519 3973
NotI GC'GGCC_GC
Cuts at: 4939 4939
Size: 7492
NspI r_CATG'y
Cuts at: 1132 1229 3001 3145 4850 6395 1132
Size: 97 1772 144 1705 1545 2229
Fragments arranged by size:
2229 1772 1705 1545 144 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7492
PciI A'CATG_T
Cuts at: 6391 6391
Size: 7492
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7492
PfoI T'CCnGG_A
Cuts at: 3396 3396
Size: 7492
PmeI GTTT'AAAC
Cuts at: 4796 4796
Size: 7492
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5561 5593 7074 7106 5561
Size: 32 1481 32 5947
Fragments arranged by size:
5947 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2048 3151 2048
Size: 1103 6389
PshAI GACnn'nnGTC
Cuts at: 1613 2489 1613
Size: 876 6616
PsiI TTA'TAA
Cuts at: 5062 5062
Size: 7492
PstI C_TGCA'G
Cuts at: 830 2239 2265 3159 3504 4844 830
Size: 1409 26 894 345 1340 3478
Fragments arranged by size:
3478 1409 1340 894 345 26
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7492
PvuII CAG'CTG
Cuts at: 2383 3823 2383
Size: 1440 6052
SalI G'TCGA_C
Cuts at: 1936 4834 1936
Size: 2898 4594
SanDI GG'GwC_CC
Cuts at: 2048 2048
Size: 7492
SbfI CC_TGCA'GG
Cuts at: 2239 4844 2239
Size: 2605 4887
ScaI AGT'ACT
Cuts at: 5740 7452 5740
Size: 1712 5780
SexAI A'CCwGG_T
Cuts at: 3048 3093 3048
Size: 45 7447
SfcI C'TryA_G
Cuts at: 826 1045 2235 2261 3155 3500 4840 5975 6656
Size: 219 1190 26 894 345 1340 1135 681
Cuts at: 6656 6847 826
Size: 191 1471
Fragments arranged by size:
1471 1340 1190 1135 894 681 345 219
191 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 3840 3840
Size: 7492
SfoI GGC'GCC
Cuts at: 2459 2459
Size: 7492
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7492
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7492
SmaI CCC'GGG
Cuts at: 1872 4819 1872
Size: 2947 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3031 5596 6497 6759 820
Size: 197 926 1088 2565 901 262 1553
Fragments arranged by size:
2565 1553 1088 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5283 493
Size: 4790 2702
SpeI A'CTAG_T
Cuts at: 152 2521 152
Size: 2369 5123
SphI G_CATG'C
Cuts at: 1132 4850 1132
Size: 3718 3774
SspI AAT'ATT
Cuts at: 5 52 3226 5416 5
Size: 47 3174 2190 2081
Fragments arranged by size:
3174 2190 2081 47
StuI AGG'CCT
Cuts at: 1517 2691 4693 1517
Size: 1174 2002 4316
Fragments arranged by size:
4316 2002 1174
StyI C'CwwG_G
Cuts at: 513 1065 2004 2024 2686 3081 4144 4931 513
Size: 552 939 20 662 395 1063 787 3074
Fragments arranged by size:
3074 1063 939 787 662 552 395 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2705 3655 5519 5536 5689 5874 6319
Size: 1255 1125 950 1864 17 153 185 445
Cuts at: 6319 325
Size: 1498
Fragments arranged by size:
1864 1498 1255 1125 950 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5738 7450 96
Size: 274 80 33 51 1443 3761 1712 138
Fragments arranged by size:
3761 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7452 7484 7452
Size: 32 7460
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7492
XbaI T'CTAG_A
Cuts at: 4828 4828
Size: 7492
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2425 2425
Size: 7492
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7492
XmaI C'CCGG_G
Cuts at: 1870 4817 1870
Size: 2947 4545
XmnI GAAnn'nnTTC
Cuts at: 2156 2732 4623 5621 2156
Size: 576 1891 998 4027
Fragments arranged by size:
4027 1891 998 576
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3331 5300 276
Size: 53 83 186 936 1797 1969 2468
Fragments arranged by size:
2468 1969 1797 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SrfI SwaI