Restriction map for
pFN21AA0461
(Circular) (Six-base) MAPSORT of: pFN21AA0461.seq Check: 9003 from: 1 to: 8776
pFN21AA0461 8776 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2734 4193 2734
Size: 1459 7317
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6586 278
Size: 53 83 186 936 5050 2468
Fragments arranged by size:
5050 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6119 1937
Size: 4182 4594
Acc65I G'GTAC_C
Cuts at: 2420 6097 2420
Size: 3677 5099
AclI AA'CG_TT
Cuts at: 1563 6903 7276 1563
Size: 5340 373 3063
Fragments arranged by size:
5340 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3027 3464 3740 4557 5723 5936 5991 6839
Size: 1395 437 276 817 1166 213 55 848
Cuts at: 6839 8223 8491 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1395 1384 1166 848 817 437 276
268 213 55
AfeI AGC'GCT
Cuts at: 5807 5807
Size: 8776
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8579
AflIII A'CryG_T
Cuts at: 1203 3024 7675 8652 1203
Size: 1821 4651 977 1327
Fragments arranged by size:
4651 1821 1327 977
AgeI A'CCGG_T
Cuts at: 7607 7607
Size: 8776
AhdI GACnn_n'nnGTC
Cuts at: 7505 7505
Size: 8776
AleI CACnn'nnGTG
Cuts at: 1166 3905 1166
Size: 2739 6037
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4397 5534 1184
Size: 3213 1137 4426
Fragments arranged by size:
4426 3213 1137
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3078 3110 4979 5011 3078
Size: 32 1869 32 6843
Fragments arranged by size:
6843 1869 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3137 5714 6519 8091 3137
Size: 2577 805 1572 3822
Fragments arranged by size:
3822 2577 1572 805
ApaLI G'TGCA_C
Cuts at: 6833 7989 6833
Size: 1156 7620
ApoI r'AATT_y
Cuts at: 1493 3301 4267 5625 6085 6311 6463 8572 1493
Size: 1808 966 1358 460 226 152 2109 1697
Fragments arranged by size:
2109 1808 1697 1358 966 460 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7330 160
Size: 872 6298 1606
Fragments arranged by size:
6298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8776
AvaI C'yCGr_G
Cuts at: 1870 1943 3924 4194 5475 5697 6101 1870
Size: 73 1981 270 1281 222 404 4545
Fragments arranged by size:
4545 1981 1281 404 270 222 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3177 3210 5235 5268 3177
Size: 33 2025 33 6685
Fragments arranged by size:
6685 2025 33 33
BamHI G'GATC_C
Cuts at: 6106 6473 6106
Size: 367 8409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2420 3765 3899 5079 6097
Size: 325 217 627 633 1345 134 1180 1018
Cuts at: 6097 7552 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1345 1180 1018 633 627 325
217 134
BanII G_rGCy'C
Cuts at: 1391 4244 4694 6095 1391
Size: 2853 450 1401 4072
Fragments arranged by size:
4072 2853 1401 450
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2904 3718 4018 5934 5987 928
Size: 971 1005 814 300 1916 53 3717
Fragments arranged by size:
3717 1916 1005 971 814 300 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6967 7001 1903
Size: 34 5030 34 3678
Fragments arranged by size:
5030 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3580 3682 3961 5236 6668 7878 3580
Size: 102 279 1275 1432 1210 4478
Fragments arranged by size:
4478 1432 1275 1210 279 102
BclI T'GATC_A
Cuts at: 1548 4215 4675 1548
Size: 2667 460 5649
Fragments arranged by size:
5649 2667 460
BfrBI ATG'CAT
Cuts at: 3743 5646 3743
Size: 1903 6873
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2506 5336 5844
Size: 107 122 71 631 605 834 2830 508
Cuts at: 5844 7387 136
Size: 1543 1525
Fragments arranged by size:
2830 1543 1525 834 631 605 508 122
107 71
BglII A'GATC_T
Cuts at: 3534 5043 5226 6061 8771 3534
Size: 1509 183 835 2710 3539
Fragments arranged by size:
3539 2710 1509 835 183
BlpI GC'TnA_GC
Cuts at: 4530 5255 6193 4530
Size: 725 938 7113
Fragments arranged by size:
7113 938 725
Bme1580I G_kGCm'C
Cuts at: 1790 2201 5300 6837 7993 1790
Size: 411 3099 1537 1156 2573
Fragments arranged by size:
3099 2573 1537 1156 411
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2419 2572 2654
Size: 416 53 83 399 24 1002 153 82
Cuts at: 2654 2933 3084 4688 6507 7465 442
Size: 279 151 1604 1819 958 1753
Fragments arranged by size:
1819 1753 1604 1002 958 416 399 279
153 151 83 82 53 24
BmtI G_CTAG'C
Cuts at: 1056 2705 4718 1056
Size: 1649 2013 5114
Fragments arranged by size:
5114 2013 1649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2652 2936 3557 3621 3902 4127 7436 1239
Size: 1413 284 621 64 281 225 3309 2579
Fragments arranged by size:
3309 2579 1413 621 284 281 225 64
Bpu10I CC'TnA_GC
Cuts at: 4555 4710 4555
Size: 155 8621
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4396 6901 7766 8064 4396
Size: 2505 865 298 5108
Fragments arranged by size:
5108 2505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4982 6017 7439 882
Size: 617 3483 1035 1422 2219
Fragments arranged by size:
3483 2219 1422 1035 617
BsaAI yAC'GTr
Cuts at: 493 1204 3394 4156 6567 493
Size: 711 2190 762 2411 2702
Fragments arranged by size:
2702 2411 2190 762 711
BsaBI GATnn'nnATC
Cuts at: 8770 8770
Size: 8776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6583 6965 7600 275
Size: 53 83 186 936 5050 382 635 1451
Fragments arranged by size:
5050 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4446 7209 7607 7881 8028 8439 4446
Size: 2763 398 274 147 411 4783
Fragments arranged by size:
4783 2763 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2030 2060 3555 3585 4748
Size: 30 165 30 627 30 1495 30 1163
Cuts at: 4748 4778 4979 5009 5453 5483 1178
Size: 30 201 30 444 30 4471
Fragments arranged by size:
4471 1495 1163 627 444 201 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2054 2057 3631 3994 4461 5000 5900
Size: 865 3 3 1574 363 467 539 900
Cuts at: 5900 5921 1186
Size: 21 4041
Fragments arranged by size:
4041 1574 900 865 539 467 363 21
3 3
BseYI C'CCAG_C
Cuts at: 2583 2603 3291 3971 4525 4561 4842 4956 5605
Size: 20 688 680 554 36 281 114 649
Cuts at: 5605 5758 7979 8475 2583
Size: 153 2221 496 2884
Fragments arranged by size:
2884 2221 688 680 649 554 496 281
153 114 36 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4132 4279 1163
Size: 573 2396 147 5660
Fragments arranged by size:
5660 2396 573 147
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6226 6987 8015 1540
Size: 275 186 4225 761 1028 2301
Fragments arranged by size:
4225 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6837 6922 7993 6837
Size: 85 1071 7620
Fragments arranged by size:
7620 1071 85
BsmI GAATG_Cn'
Cuts at: 2251 3743 5149 5284 6287 6380 2251
Size: 1492 1406 135 1003 93 4647
Fragments arranged by size:
4647 1492 1406 1003 135 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 4244 4694 5300 6095 6837 6922
Size: 399 411 2043 450 606 795 742 85
Cuts at: 6922 7993 1391
Size: 1071 2174
Fragments arranged by size:
2174 2043 1071 795 742 606 450 411
399 85
BspEI T'CCGG_A
Cuts at: 8439 8439
Size: 8776
BspHI T'CATG_A
Cuts at: 3372 6665 8395 3372
Size: 3293 1730 3753
Fragments arranged by size:
3753 3293 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2734 4193 6131 844
Size: 1890 1459 1938 3489
Fragments arranged by size:
3489 1938 1890 1459
BsrBI CCG'CTC
Cuts at: 1394 1823 6663 1394
Size: 429 4840 3507
Fragments arranged by size:
4840 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2349 2736 2794 3745 7271 7445 65
Size: 1183 1101 387 58 951 3526 174 1396
Fragments arranged by size:
3526 1396 1183 1101 951 387 174 58
BsrFI r'CCGG_y
Cuts at: 1700 1810 3801 4092 7420 7607 1700
Size: 110 1991 291 3328 187 2869
Fragments arranged by size:
3328 2869 1991 291 187 110
BsrGI T'GTAC_A
Cuts at: 96 2332 5167 96
Size: 2236 2835 3705
Fragments arranged by size:
3705 2835 2236
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8776
BssSI C'ACGA_G
Cuts at: 4428 6836 7848 4428
Size: 2408 1012 5356
Fragments arranged by size:
5356 2408 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3798 5144 5714 794
Size: 3004 1346 570 3856
Fragments arranged by size:
3856 3004 1346 570
BstBI TT'CG_AA
Cuts at: 2557 7580 2557
Size: 5023 3753
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8776
BstXI CCAn_nnnn'nTGG
Cuts at: 2153 2846 2960 3880 4252 4345 2153
Size: 693 114 920 372 93 6584
Fragments arranged by size:
6584 920 693 372 114 93
BstYI r'GATC_y
Cuts at: 1972 3534 4122 4367 5043 5226 6061 6106 6473
Size: 1562 588 245 676 183 835 45 367
Cuts at: 6473 6858 6875 8327 8413 8425 8771 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1562 1452 835 676 588 385 367
346 245 183 86 45 17 12
Bsu36I CC'TnA_GG
Cuts at: 3309 5127 3309
Size: 1818 6958
BtgI C'CryG_G
Cuts at: 513 1065 2004 2054 4232 4385 513
Size: 552 939 50 2178 153 4904
Fragments arranged by size:
4904 2178 939 552 153 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3234 4341 5522 487
Size: 683 56 2008 1107 1181 3741
Fragments arranged by size:
3741 2008 1181 1107 683 56
BtsI GCAGTG_nn'
Cuts at: 2622 2736 3787 4159 5266 5604 6292 7086 7106
Size: 114 1051 372 1107 338 688 794 20
Cuts at: 7106 2622
Size: 4292
Fragments arranged by size:
4292 1107 1051 794 688 372 338 114
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8741
DraI TTT'AAA
Cuts at: 4264 6080 4264
Size: 1816 6960
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7783 809
Size: 507 6467 1802
Fragments arranged by size:
6467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2541 2796 4335
Size: 54 1425 50 275 18 711 255 1539
Cuts at: 4335 4420 6223 7112 8741 8
Size: 85 1803 889 1629 43
Fragments arranged by size:
1803 1629 1539 1425 889 711 275 255
85 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6223 1537
Size: 275 4411 4090
Fragments arranged by size:
4411 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2935 3160 3767 4055 4232 4533 4923 5159
Size: 1582 225 607 288 177 301 390 236
Cuts at: 5159 5550 5967 6706 1353
Size: 391 417 739 3423
Fragments arranged by size:
3423 1582 739 607 417 391 390 301
288 236 225 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 7363 7737 7883 387
Size: 1064 138 435 5339 374 146 1280
Fragments arranged by size:
5339 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2652 2936 3027 3464 3557 3621 3740
Size: 393 1020 284 91 437 93 64 119
Cuts at: 3740 3902 4127 4557 5723 5936 5991 6839 7436
Size: 162 225 430 1166 213 55 848 597
Cuts at: 7436 8223 8491 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1166 1020 848 787 597 437 430
393 284 268 225 213 162 119 93
91 64 55
EcoNI CCTnn'n_nnAGG
Cuts at: 5126 5126
Size: 8776
EcoRI G'AATT_C
Cuts at: 6085 6463 6085
Size: 378 8398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5537 5537
Size: 8776
FspI TGC'GCA
Cuts at: 7282 7282
Size: 8776
HaeII r_GCGC'y
Cuts at: 1678 3978 5809 7923 1678
Size: 2300 1831 2114 2531
Fragments arranged by size:
2531 2300 2114 1831
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2148 2164 2180 2196 2677
Size: 32 135 32 576 16 16 16 481
Cuts at: 2677 2709 4207 4239 4703 4735 4748 4780 5213
Size: 32 1498 32 464 32 13 32 433
Cuts at: 5213 5245 5453 5485 5518 5550 6014 6046 7417
Size: 32 208 32 33 32 464 32 1371
Cuts at: 7417 7449 7491 7523 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1498 1371 576 481 464 464 433
208 135 42 33 32 32 32 32
32 32 32 32 32 32 32 32
16 16 16 13
HincII GTy'rAC
Cuts at: 669 1657 1938 5472 6120 6366 669
Size: 988 281 3534 648 246 3079
Fragments arranged by size:
3534 3079 988 648 281 246
HindIII A'AGCT_T
Cuts at: 748 3009 3842 4775 748
Size: 2261 833 933 4749
Fragments arranged by size:
4749 2261 933 833
HpaI GTT'AAC
Cuts at: 6366 6366
Size: 8776
KpnI G_GTAC'C
Cuts at: 2424 6101 2424
Size: 3677 5099
MfeI C'AATT_G
Cuts at: 5979 6375 5979
Size: 396 8380
MluI A'CGCG_T
Cuts at: 8652 8652
Size: 8776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2028 2425 2889 3772 7890 8074 1305
Size: 723 397 464 883 4118 184 2007
Fragments arranged by size:
4118 2007 883 723 464 397 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2798 4337 4422 10
Size: 54 1425 343 966 1539 85 4364
Fragments arranged by size:
4364 1539 1425 966 343 85 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3905 5522 6734 7093 7252 518
Size: 648 2739 1617 1212 359 159 2042
Fragments arranged by size:
2739 2042 1617 1212 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2129 2901 3880 3997 4529 4639 4762 4823
Size: 523 772 979 117 532 110 123 61
Cuts at: 4823 5743 6192 6869 8017 8262 1606
Size: 920 449 677 1148 245 2120
Fragments arranged by size:
2120 1148 979 920 772 677 532 523
449 245 123 117 110 61
NaeI GCC'GGC
Cuts at: 3803 4094 3803
Size: 291 8485
NcoI C'CATG_G
Cuts at: 513 1065 2004 2054 513
Size: 552 939 50 7235
Fragments arranged by size:
7235 939 552 50
NdeI CA'TA_TG
Cuts at: 387 3381 3579 387
Size: 2994 198 5584
Fragments arranged by size:
5584 2994 198
NgoMIV G'CCGG_C
Cuts at: 3801 4092 3801
Size: 291 8485
NheI G'CTAG_C
Cuts at: 1052 2701 4714 1052
Size: 1649 2013 5114
Fragments arranged by size:
5114 2013 1649
NotI GC'GGCC_GC
Cuts at: 6223 6223
Size: 8776
NsiI A_TGCA'T
Cuts at: 3745 5648 3745
Size: 1903 6873
NspI r_CATG'y
Cuts at: 1132 1229 3028 4104 5404 5521 5721 5881 6134
Size: 97 1799 1076 1300 117 200 160 253
Cuts at: 6134 7679 1132
Size: 1545 2229
Fragments arranged by size:
2229 1799 1545 1300 1076 253 200 160
117 97
PasI CC'CwG_GG
Cuts at: 1348 2833 4885 1348
Size: 1485 2052 5239
Fragments arranged by size:
5239 2052 1485
PciI A'CATG_T
Cuts at: 3024 7675 3024
Size: 4651 4125
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8776
PfoI T'CCnGG_A
Cuts at: 2952 5238 2952
Size: 2286 6490
PmeI GTTT'AAAC
Cuts at: 6080 6080
Size: 8776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4979 5011 6845 6877 8358 8390 4979
Size: 32 1834 32 1481 32 5365
Fragments arranged by size:
5365 1834 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2908 1613
Size: 1295 7481
PsiI TTA'TAA
Cuts at: 2103 6346 2103
Size: 4243 4533
PspXI vC'TCGA_Gb
Cuts at: 5475 5475
Size: 8776
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4956 4988 4956
Size: 32 8744
PstI C_TGCA'G
Cuts at: 830 2731 3810 5289 5599 6128 830
Size: 1901 1079 1479 310 529 3478
Fragments arranged by size:
3478 1901 1479 1079 529 310
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8776
PvuII CAG'CTG
Cuts at: 2129 3880 4529 4762 4823 5743 2129
Size: 1751 649 233 61 920 5162
Fragments arranged by size:
5162 1751 920 649 233 61
SalI G'TCGA_C
Cuts at: 1936 6118 1936
Size: 4182 4594
SapI GCTCTTCn'nnn_
Cuts at: 4232 4533 4232
Size: 301 8475
SbfI CC_TGCA'GG
Cuts at: 6128 6128
Size: 8776
ScaI AGT'ACT
Cuts at: 4788 7024 8736 4788
Size: 2236 1712 4828
Fragments arranged by size:
4828 2236 1712
SexAI A'CCwGG_T
Cuts at: 2764 2764
Size: 8776
SfcI C'TryA_G
Cuts at: 826 1045 2088 2727 3806 4177 4644 5285 5371
Size: 219 1043 639 1079 371 467 641 86
Cuts at: 5371 5440 5595 5614 6124 7259 7940 8131 826
Size: 69 155 19 510 1135 681 191 1471
Fragments arranged by size:
1471 1135 1079 1043 681 641 639 510
467 371 219 191 155 86 69 19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8776
SmaI CCC'GGG
Cuts at: 1872 6103 1872
Size: 4231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4194 4411 5475 6880 7781 8043
Size: 197 926 2251 217 1064 1405 901 262
Cuts at: 8043 820
Size: 1553
Fragments arranged by size:
2251 1553 1405 1064 926 901 262 217
197
SnaBI TAC'GTA
Cuts at: 493 4156 6567 493
Size: 3663 2411 2702
Fragments arranged by size:
3663 2702 2411
SpeI A'CTAG_T
Cuts at: 152 2261 2404 152
Size: 2109 143 6524
Fragments arranged by size:
6524 2109 143
SphI G_CATG'C
Cuts at: 1132 5521 6134 1132
Size: 4389 613 3774
Fragments arranged by size:
4389 3774 613
SspI AAT'ATT
Cuts at: 5 52 2289 3364 6700 5
Size: 47 2237 1075 3336 2081
Fragments arranged by size:
3336 2237 2081 1075 47
StuI AGG'CCT
Cuts at: 1517 2227 4880 1517
Size: 710 2653 5413
Fragments arranged by size:
5413 2653 710
StyI C'CwwG_G
Cuts at: 513 1065 2004 2054 3822 3903 5309 6215 513
Size: 552 939 50 1768 81 1406 906 3074
Fragments arranged by size:
3074 1768 1406 939 906 552 81 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4449 4715 4936 5750 6041 6803 6820
Size: 1255 2869 266 221 814 291 762 17
Cuts at: 6820 6973 7158 7603 325
Size: 153 185 445 1498
Fragments arranged by size:
2869 1498 1255 814 762 445 291 266
221 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2332 4786 5167
Size: 274 80 33 51 1443 355 2454 381
Cuts at: 5167 7022 8734 96
Size: 1855 1712 138
Fragments arranged by size:
2454 1855 1712 1443 381 355 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3555 3587 4066 4098 5297 5329 5338 5370 8736
Size: 32 479 32 1199 32 9 32 3366
Cuts at: 8736 8768 3555
Size: 32 3563
Fragments arranged by size:
3563 3366 1199 479 32 32 32 32
32 9
Tth111I GACn'n_nGTC
Cuts at: 1316 2593 6028 1316
Size: 1277 3435 4064
Fragments arranged by size:
4064 3435 1277
XbaI T'CTAG_A
Cuts at: 4897 6112 4897
Size: 1215 7561
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2830 2830
Size: 8776
XhoI C'TCGA_G
Cuts at: 1943 4194 5475 1943
Size: 2251 1281 5244
Fragments arranged by size:
5244 2251 1281
XmaI C'CCGG_G
Cuts at: 1870 6101 1870
Size: 4231 4545
XmnI GAAnn'nnTTC
Cuts at: 3498 3725 5994 6905 3498
Size: 227 2269 911 5369
Fragments arranged by size:
5369 2269 911 227
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6584 276
Size: 53 83 186 936 5050 2468
Fragments arranged by size:
5050 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI
NarI NruI PacI PmlI PpuMI PspOMI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI