Restriction map for
pFN21AA0459
(Circular) (Six-base) MAPSORT of: pFN21AA0459.seq Check: 2573 from: 1 to: 5899
pFN21AA0459 5899 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2599 2599
Size: 5899
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3709 278
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3242 1937
Size: 1305 4594
Acc65I G'GTAC_C
Cuts at: 3220 3220
Size: 5899
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2219 2697 2897 3962 5346 5614 1632
Size: 587 478 200 1065 1384 268 1917
Fragments arranged by size:
1917 1384 1065 587 478 268 200
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5702
AflIII A'CryG_T
Cuts at: 1203 4798 5775 1203
Size: 3595 977 1327
Fragments arranged by size:
3595 1327 977
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 4628 4628
Size: 5899
AleI CACnn'nnGTG
Cuts at: 1166 3170 1166
Size: 2004 3895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5899
AlwNI CAG_nnn'CTG
Cuts at: 2191 2767 3642 5214 2191
Size: 576 875 1572 2876
Fragments arranged by size:
2876 1572 875 576
ApaI G_GGCC'C
Cuts at: 2101 2101
Size: 5899
ApaLI G'TGCA_C
Cuts at: 3956 5112 3956
Size: 1156 4743
ApoI r'AATT_y
Cuts at: 1493 3208 3434 3586 5695 1493
Size: 1715 226 152 2109 1697
Fragments arranged by size:
2109 1715 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2090 2090
Size: 5899
AseI AT'TA_AT
Cuts at: 160 1032 4453 160
Size: 872 3421 1606
Fragments arranged by size:
3421 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 3224 1870
Size: 73 1281 4545
Fragments arranged by size:
4545 1281 73
BamHI G'GATC_C
Cuts at: 3229 3596 3229
Size: 367 5532
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2212 2587 2915 3087 3220
Size: 325 217 627 425 375 328 172 133
Cuts at: 3220 4675 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 425 375 328 325 217
172 133
BanII G_rGCy'C
Cuts at: 1391 2101 2245 2862 3218 1391
Size: 710 144 617 356 4072
Fragments arranged by size:
4072 710 617 356 144
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2685 3182 928
Size: 971 786 497 3645
Fragments arranged by size:
3645 971 786 497
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4090 4124 1903
Size: 34 2153 34 3678
Fragments arranged by size:
3678 2153 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3791 5001 3791
Size: 1210 4689
BclI T'GATC_A
Cuts at: 1548 2251 1548
Size: 703 5196
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 2384 4510
Size: 107 122 71 631 605 365 347 2126
Cuts at: 4510 136
Size: 1525
Fragments arranged by size:
2126 1525 631 605 365 347 122 107
71
BglII A'GATC_T
Cuts at: 5894 5894
Size: 5899
BlpI GC'TnA_GC
Cuts at: 3316 3316
Size: 5899
Bme1580I G_kGCm'C
Cuts at: 1790 2101 3960 5116 1790
Size: 311 1859 1156 2573
Fragments arranged by size:
2573 1859 1156 311
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5899
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2856 3630 4588
Size: 416 53 83 399 24 1439 774 958
Cuts at: 4588 442
Size: 1753
Fragments arranged by size:
1753 1439 958 774 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5899
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 5899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2585 3125 4559 1239
Size: 1346 540 1434 2579
Fragments arranged by size:
2579 1434 1346 540
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2267 2876 4024 4889 5187 2267
Size: 609 1148 865 298 2979
Fragments arranged by size:
2979 1148 865 609 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4562 882
Size: 617 3063 2219
Fragments arranged by size:
3063 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3690 493
Size: 711 2486 2702
Fragments arranged by size:
2702 2486 711
BsaBI GATnn'nnATC
Cuts at: 5893 5893
Size: 5899
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3706 4088 4723 275
Size: 53 83 186 936 2173 382 635 1451
Fragments arranged by size:
2173 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4332 4730 5004 5151 5562 4332
Size: 398 274 147 411 4669
Fragments arranged by size:
4669 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5674
Fragments arranged by size:
5674 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 2171 2915 3095 1186
Size: 982 3 744 180 3990
Fragments arranged by size:
3990 982 744 180 3
BseYI C'CCAG_C
Cuts at: 5102 5598 5102
Size: 496 5403
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2577 1163
Size: 573 841 4485
Fragments arranged by size:
4485 841 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2036 2126 3349 4110 5138 1540
Size: 275 186 35 90 1223 761 1028 2301
Fragments arranged by size:
2301 1223 1028 761 275 186 90 35
BsiHKAI G_wGCw'C
Cuts at: 2245 2550 3960 4045 5116 2245
Size: 305 1410 85 1071 3028
Fragments arranged by size:
3028 1410 1071 305 85
BsmI GAATG_Cn'
Cuts at: 3410 3503 3410
Size: 93 5806
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2245 2550 2862 3218 3960 4045
Size: 399 311 144 305 312 356 742 85
Cuts at: 4045 5116 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 356 312 311 305
144 85
BspEI T'CCGG_A
Cuts at: 5562 5562
Size: 5899
BspHI T'CATG_A
Cuts at: 3788 5518 3788
Size: 1730 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2599 3151 3254 844
Size: 1755 552 103 3489
Fragments arranged by size:
3489 1755 552 103
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 2113 3188 3786 1394
Size: 429 260 30 1075 598 3507
Fragments arranged by size:
3507 1075 598 429 260 30
BsrDI GCAATG_nn'
Cuts at: 65 1248 2416 4394 4568 65
Size: 1183 1168 1978 174 1396
Fragments arranged by size:
1978 1396 1183 1168 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2031 4543 4730 1700
Size: 110 221 2512 187 2869
Fragments arranged by size:
2869 2512 221 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5899
BssHII G'CGCG_C
Cuts at: 1925 2090 1925
Size: 165 5734
BssSI C'ACGA_G
Cuts at: 3959 4971 3959
Size: 1012 4887
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2804 794
Size: 2010 3889
BstBI TT'CG_AA
Cuts at: 4703 4703
Size: 5899
BstEII G'GTnAC_C
Cuts at: 1184 3163 1184
Size: 1979 3920
BstYI r'GATC_y
Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894
Size: 1257 367 385 17 1452 86 12 346
Cuts at: 5894 1972
Size: 1977
Fragments arranged by size:
1977 1452 1257 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5347
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2512 487
Size: 683 56 1286 3874
Fragments arranged by size:
3874 1286 683 56
BtsI GCAGTG_nn'
Cuts at: 2888 2917 3059 3415 4209 4229 2888
Size: 29 142 356 794 20 4558
Fragments arranged by size:
4558 794 356 142 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5864
DraI TTT'AAA
Cuts at: 3203 3203
Size: 5899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4906 809
Size: 507 3590 1802
Fragments arranged by size:
3590 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2033 2103 2123
Size: 54 1425 50 275 18 203 70 20
Cuts at: 2123 2376 3346 4235 5864 8
Size: 253 970 889 1629 43
Fragments arranged by size:
1629 1425 970 889 275 253 203 70
54 50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2033 2123 3346 1537
Size: 275 221 90 1223 4090
Fragments arranged by size:
4090 1223 275 221 90
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2825 2841 3829 1353
Size: 668 804 16 988 3423
Fragments arranged by size:
3423 988 804 668 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2131 4486 4860 5006 387
Size: 1064 138 542 2355 374 146 1280
Fragments arranged by size:
2355 1280 1064 542 374 146 138
EcoICRI GAG'CTC
Cuts at: 2243 2243
Size: 5899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2585 2697 2897 3125 3962 4559
Size: 393 587 366 112 200 228 837 597
Cuts at: 4559 5346 5614 1239
Size: 787 268 1524
Fragments arranged by size:
1524 837 787 597 587 393 366 268
228 200 112
EcoNI CCTnn'n_nnAGG
Cuts at: 2105 2105
Size: 5899
EcoO109I rG'GnC_Cy
Cuts at: 2098 2188 2098
Size: 90 5809
EcoRI G'AATT_C
Cuts at: 3208 3586 3208
Size: 378 5521
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2870 2870
Size: 5899
FspI TGC'GCA
Cuts at: 4405 4405
Size: 5899
HaeII r_GCGC'y
Cuts at: 1678 5046 1678
Size: 3368 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2608 2637 2640 2669 4540
Size: 32 135 32 1036 29 3 29 1871
Cuts at: 4540 4572 4614 4646 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1871 1036 135 42 32 32 32
32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3243 3489 669
Size: 988 281 1305 246 3079
Fragments arranged by size:
3079 1305 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5899
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KpnI G_GTAC'C
Cuts at: 3224 3224
Size: 5899
MfeI C'AATT_G
Cuts at: 2227 2255 2418 3498 2227
Size: 28 163 1080 4628
Fragments arranged by size:
4628 1080 163 28
MluI A'CGCG_T
Cuts at: 5775 5775
Size: 5899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5013 5197 1305
Size: 3708 184 2007
Fragments arranged by size:
3708 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2105 2378 10
Size: 54 1425 343 273 273 3531
Fragments arranged by size:
3531 1425 343 273 273 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2628 3170 3857 4216 4375 518
Size: 648 1462 542 687 359 159 2042
Fragments arranged by size:
2042 1462 687 648 542 359 159
MspA1I CmG'CkG
Cuts at: 1606 2043 2182 2323 3315 3992 5140 5385 1606
Size: 437 139 141 992 677 1148 245 2120
Fragments arranged by size:
2120 1148 992 677 437 245 141 139
NaeI GCC'GGC
Cuts at: 2033 2033
Size: 5899
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5347
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NgoMIV G'CCGG_C
Cuts at: 2031 2031
Size: 5899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5899
NotI GC'GGCC_GC
Cuts at: 3346 3346
Size: 5899
NspI r_CATG'y
Cuts at: 1132 1229 2375 3257 4802 1132
Size: 97 1146 882 1545 2229
Fragments arranged by size:
2229 1545 1146 882 97
PasI CC'CwG_GG
Cuts at: 1348 2191 1348
Size: 843 5056
PciI A'CATG_T
Cuts at: 4798 4798
Size: 5899
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2767 1108
Size: 1659 4240
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2144 2176 3968 4000 5481 5513 2144
Size: 32 1792 32 1481 32 2530
Fragments arranged by size:
2530 1792 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5899
PsiI TTA'TAA
Cuts at: 2784 3469 2784
Size: 685 5214
PspOMI G'GGCC_C
Cuts at: 2097 2097
Size: 5899
PstI C_TGCA'G
Cuts at: 830 2187 3251 830
Size: 1357 1064 3478
Fragments arranged by size:
3478 1357 1064
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
PvuII CAG'CTG
Cuts at: 2182 2182
Size: 5899
SacI G_AGCT'C
Cuts at: 2245 2245
Size: 5899
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SapI GCTCTTCn'nnn_
Cuts at: 2021 2021
Size: 5899
SbfI CC_TGCA'GG
Cuts at: 3251 3251
Size: 5899
ScaI AGT'ACT
Cuts at: 4147 5859 4147
Size: 1712 4187
SfcI C'TryA_G
Cuts at: 826 1045 2183 3247 4382 5063 5254 826
Size: 219 1138 1064 1135 681 191 1471
Fragments arranged by size:
1471 1138 1135 1064 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5899
SmaI CCC'GGG
Cuts at: 1872 3226 1872
Size: 1354 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2282 2855 4003 4904 5166 820
Size: 197 926 339 573 1148 901 262 1553
Fragments arranged by size:
1553 1148 926 901 573 339 262 197
SnaBI TAC'GTA
Cuts at: 493 3690 493
Size: 3197 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 3257 1132
Size: 2125 3774
SspI AAT'ATT
Cuts at: 5 52 2788 3823 5
Size: 47 2736 1035 2081
Fragments arranged by size:
2736 2081 1035 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5899
StyI C'CwwG_G
Cuts at: 513 1065 2106 2824 3168 3338 513
Size: 552 1041 718 344 170 3074
Fragments arranged by size:
3074 1041 718 552 344 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2972 3926 3943 4096 4281 4726 325
Size: 1255 1392 954 17 153 185 445 1498
Fragments arranged by size:
1498 1392 1255 954 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4145 5857 96
Size: 274 80 33 51 1443 2168 1712 138
Fragments arranged by size:
2168 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5859 5891 5859
Size: 32 5867
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5899
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5899
XmaI C'CCGG_G
Cuts at: 1870 3224 1870
Size: 1354 4545
XmnI GAAnn'nnTTC
Cuts at: 3179 4028 3179
Size: 849 5050
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3707 276
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI