Restriction map for
pFN21AA0455
(Circular) (Six-base) MAPSORT of: pFN21AA0455.seq Check: 5886 from: 1 to: 6994
pFN21AA0455 6994 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4804 278
Size: 53 83 186 936 3268 2468
Fragments arranged by size:
3268 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4337 1937
Size: 2400 4594
Acc65I G'GTAC_C
Cuts at: 4315 4315
Size: 6994
AclI AA'CG_TT
Cuts at: 1563 5121 5494 1563
Size: 3558 373 3063
Fragments arranged by size:
3558 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3226 3813 3942 5057 6441 6709 1632
Size: 1594 587 129 1115 1384 268 1917
Fragments arranged by size:
1917 1594 1384 1115 587 268 129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6797
AflIII A'CryG_T
Cuts at: 1203 5893 6870 1203
Size: 4690 977 1327
Fragments arranged by size:
4690 1327 977
AgeI A'CCGG_T
Cuts at: 5825 5825
Size: 6994
AhdI GACnn_n'nnGTC
Cuts at: 2528 5723 2528
Size: 3195 3799
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6994
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3269 1184
Size: 2085 4909
AlwNI CAG_nnn'CTG
Cuts at: 2711 4737 6309 2711
Size: 2026 1572 3396
Fragments arranged by size:
3396 2026 1572
ApaLI G'TGCA_C
Cuts at: 2102 2294 3605 5051 6207 2102
Size: 192 1311 1446 1156 2889
Fragments arranged by size:
2889 1446 1311 1156 192
ApoI r'AATT_y
Cuts at: 1493 2430 2677 2989 4303 4529 4681 6790 1493
Size: 937 247 312 1314 226 152 2109 1697
Fragments arranged by size:
2109 1697 1314 937 312 247 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2487 5548 160
Size: 872 1455 3061 1606
Fragments arranged by size:
3061 1606 1455 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
AvaI C'yCGr_G
Cuts at: 1870 1943 2114 2132 3663 4319 1870
Size: 73 171 18 1531 656 4545
Fragments arranged by size:
4545 1531 656 171 73 18
BamHI G'GATC_C
Cuts at: 4324 4691 4324
Size: 367 6627
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 4315 5770 618
Size: 325 217 627 267 2261 1455 1842
Fragments arranged by size:
2261 1842 1455 627 325 267 217
BanII G_rGCy'C
Cuts at: 1391 2481 3215 3855 3993 4313 1391
Size: 1090 734 640 138 320 4072
Fragments arranged by size:
4072 1090 734 640 320 138
BbeI G_GCGC'C
Cuts at: 2058 2058
Size: 6994
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2275 2845 3288 928
Size: 971 376 570 443 4634
Fragments arranged by size:
4634 971 570 443 376
BbvCI CC'TCA_GC
Cuts at: 2090 2090
Size: 6994
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2133 2167 5185 5219 1903
Size: 34 50 34 112 34 3018 34 3678
Fragments arranged by size:
3678 3018 112 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4886 6096 4886
Size: 1210 5784
BclI T'GATC_A
Cuts at: 1548 2181 1548
Size: 633 6361
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3559 5605 136
Size: 107 122 71 631 605 1887 2046 1525
Fragments arranged by size:
2046 1887 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6989 6989
Size: 6994
BlpI GC'TnA_GC
Cuts at: 2381 4411 2381
Size: 2030 4964
Bme1580I G_kGCm'C
Cuts at: 1790 2106 2298 3609 5055 6211 1790
Size: 316 192 1311 1446 1156 2573
Fragments arranged by size:
2573 1446 1311 1156 316 192
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6994
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2912 2994 3346
Size: 416 53 83 399 24 1495 82 352
Cuts at: 3346 3849 4118 4725 5683 442
Size: 503 269 607 958 1753
Fragments arranged by size:
1753 1495 958 607 503 416 399 352
269 83 82 53 24
BmtI G_CTAG'C
Cuts at: 1056 3156 1056
Size: 2100 4894
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3050 3050
Size: 6994
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2005 2093 2514 3576 4071 5654 1239
Size: 766 88 421 1062 495 1583 2579
Fragments arranged by size:
2579 1583 1062 766 495 421 88
Bpu10I CC'TnA_GC
Cuts at: 2090 2704 3405 2090
Size: 614 701 5679
Fragments arranged by size:
5679 701 614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5119 5984 6282 5119
Size: 865 298 5831
Fragments arranged by size:
5831 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3045 3474 3811 5657 882
Size: 617 1546 429 337 1846 2219
Fragments arranged by size:
2219 1846 1546 617 429 337
BsaAI yAC'GTr
Cuts at: 493 1204 4785 493
Size: 711 3581 2702
Fragments arranged by size:
3581 2702 711
BsaBI GATnn'nnATC
Cuts at: 6988 6988
Size: 6994
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 3030 4801 5183
Size: 53 83 186 936 522 975 1771 382
Cuts at: 5183 5818 275
Size: 635 1451
Fragments arranged by size:
1771 1451 975 936 635 522 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2319 5427 5825 6099 6246 6657 2319
Size: 3108 398 274 147 411 2656
Fragments arranged by size:
3108 2656 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6769
Fragments arranged by size:
6769 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2964 3335 3632 1186
Size: 904 874 371 297 4548
Fragments arranged by size:
4548 904 874 371 297
BseYI C'CCAG_C
Cuts at: 6197 6693 6197
Size: 496 6498
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3796 1163
Size: 573 2060 4361
Fragments arranged by size:
4361 2060 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4444 5205 6233 1540
Size: 275 186 2443 761 1028 2301
Fragments arranged by size:
2443 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 2298 3609 3993 5055 5140 6211 2106
Size: 192 1311 384 1062 85 1071 2889
Fragments arranged by size:
2889 1311 1071 1062 384 192 85
BsmI GAATG_Cn'
Cuts at: 3306 4505 4598 3306
Size: 1199 93 5702
Fragments arranged by size:
5702 1199 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3021 4145 3021
Size: 1124 5870
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2298 2481 3215 3609 3855 3993
Size: 399 316 192 183 734 394 246 138
Cuts at: 3993 4313 5055 5140 6211 1391
Size: 320 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 734 399 394 320 316
246 192 183 138 85
BspEI T'CCGG_A
Cuts at: 6657 6657
Size: 6994
BspHI T'CATG_A
Cuts at: 4883 6613 4883
Size: 1730 5264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2410 4349 844
Size: 1566 1939 3489
Fragments arranged by size:
3489 1939 1566
BsrBI CCG'CTC
Cuts at: 1394 1823 3329 4881 1394
Size: 429 1506 1552 3507
Fragments arranged by size:
3507 1552 1506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4087 5489 5663 65
Size: 1183 2839 1402 174 1396
Fragments arranged by size:
2839 1402 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5638 5825 1700
Size: 110 3828 187 2869
Fragments arranged by size:
3828 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6994
BssHII G'CGCG_C
Cuts at: 1925 2011 1925
Size: 86 6908
BssSI C'ACGA_G
Cuts at: 5054 6066 5054
Size: 1012 5982
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2768 794
Size: 1974 5020
BstBI TT'CG_AA
Cuts at: 5798 5798
Size: 6994
BstEII G'GTnAC_C
Cuts at: 1184 3235 1184
Size: 2051 4943
BstXI CCAn_nnnn'nTGG
Cuts at: 3494 3494
Size: 6994
BstYI r'GATC_y
Cuts at: 1972 2708 3061 3428 3909 4324 4691 5076 5093
Size: 736 353 367 481 415 367 385 17
Cuts at: 5093 6545 6631 6643 6989 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 736 481 415 385 367 367
353 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3035 3615 3035
Size: 580 6414
BtgI C'CryG_G
Cuts at: 513 1065 2032 3206 3216 3487 4096 513
Size: 552 967 1174 10 271 609 3411
Fragments arranged by size:
3411 1174 967 609 552 271 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6255
Fragments arranged by size:
6255 683 56
BtsI GCAGTG_nn'
Cuts at: 3106 3940 4510 5304 5324 3106
Size: 834 570 794 20 4776
Fragments arranged by size:
4776 834 794 570 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6994
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6959
DraI TTT'AAA
Cuts at: 4298 4298
Size: 6994
DraIII CAC_nnn'GTG
Cuts at: 3354 3354
Size: 6994
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6001 809
Size: 507 4685 1802
Fragments arranged by size:
4685 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 4441 5330
Size: 54 1425 50 275 18 199 2412 889
Cuts at: 5330 6959 8
Size: 1629 43
Fragments arranged by size:
2412 1629 1425 889 275 199 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4441 1537
Size: 275 2629 4090
Fragments arranged by size:
4090 2629 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4924 1353
Size: 3571 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5581 5955 6101 387
Size: 1064 138 3992 374 146 1280
Fragments arranged by size:
3992 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3991 3991
Size: 6994
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2005 2093 2514 3226 3576 3813 3942
Size: 393 373 88 421 712 350 237 129
Cuts at: 3942 4071 5057 5654 6441 6709 1239
Size: 129 986 597 787 268 1524
Fragments arranged by size:
1524 986 787 712 597 421 393 373
350 268 237 129 129 88
EcoO109I rG'GnC_Cy
Cuts at: 3591 3591
Size: 6994
EcoRI G'AATT_C
Cuts at: 2430 4303 4681 2430
Size: 1873 378 4743
Fragments arranged by size:
4743 1873 378
EcoRV GAT'ATC
Cuts at: 2587 2609 2587
Size: 22 6972
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3403 3403
Size: 6994
FspI TGC'GCA
Cuts at: 5500 5500
Size: 6994
HaeII r_GCGC'y
Cuts at: 1678 2058 2065 3528 6141 1678
Size: 380 7 1463 2613 2531
Fragments arranged by size:
2613 2531 1463 380 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2514 2546 2687 2700 2719
Size: 32 135 32 942 32 141 13 19
Cuts at: 2719 2732 3140 3172 4142 4174 5635 5667 5709
Size: 13 408 32 970 32 1461 32 42
Cuts at: 5709 5741 1373
Size: 32 2626
Fragments arranged by size:
2626 1461 970 942 408 141 135 42
32 32 32 32 32 32 32 19
13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 4338 4584 669
Size: 988 281 2400 246 3079
Fragments arranged by size:
3079 2400 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2831 748
Size: 2083 4911
HpaI GTT'AAC
Cuts at: 4584 4584
Size: 6994
KasI G'GCGC_C
Cuts at: 2054 2054
Size: 6994
KpnI G_GTAC'C
Cuts at: 4319 4319
Size: 6994
MfeI C'AATT_G
Cuts at: 4593 4593
Size: 6994
MluI A'CGCG_T
Cuts at: 6870 6870
Size: 6994
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2449 6108 6292 1305
Size: 1144 3659 184 2007
Fragments arranged by size:
3659 2007 1144 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5172
Fragments arranged by size:
5172 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3147 3437 3746 4952 5311 5470 518
Size: 648 1981 290 309 1206 359 159 2042
Fragments arranged by size:
2042 1981 1206 648 359 309 290 159
MspA1I CmG'CkG
Cuts at: 1606 2025 2034 2052 2094 2596 2783 4410 5087
Size: 419 9 18 42 502 187 1627 677
Cuts at: 5087 6235 6480 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1627 1148 677 502 419 245 187
42 18 9
NarI GG'CG_CC
Cuts at: 2055 2055
Size: 6994
NcoI C'CATG_G
Cuts at: 513 1065 3216 3487 513
Size: 552 2151 271 4020
Fragments arranged by size:
4020 2151 552 271
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6994
NheI G'CTAG_C
Cuts at: 1052 3152 1052
Size: 2100 4894
NotI GC'GGCC_GC
Cuts at: 4441 4441
Size: 6994
NspI r_CATG'y
Cuts at: 1132 1229 2335 2762 3553 4352 5897 1132
Size: 97 1106 427 791 799 1545 2229
Fragments arranged by size:
2229 1545 1106 799 791 427 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6994
PciI A'CATG_T
Cuts at: 5893 5893
Size: 6994
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6994
PfoI T'CCnGG_A
Cuts at: 3941 3941
Size: 6994
PmeI GTTT'AAAC
Cuts at: 4298 4298
Size: 6994
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5063 5095 6576 6608 5063
Size: 32 1481 32 5449
Fragments arranged by size:
5449 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3591 3591
Size: 6994
PshAI GACnn'nnGTC
Cuts at: 1613 2322 1613
Size: 709 6285
PsiI TTA'TAA
Cuts at: 4287 4564 4287
Size: 277 6717
PstI C_TGCA'G
Cuts at: 830 4346 830
Size: 3516 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6994
PvuII CAG'CTG
Cuts at: 2094 2596 2783 2094
Size: 502 187 6305
Fragments arranged by size:
6305 502 187
SacI G_AGCT'C
Cuts at: 3993 3993
Size: 6994
SacII CC_GC'GG
Cuts at: 2035 2035
Size: 6994
SalI G'TCGA_C
Cuts at: 1936 4336 1936
Size: 2400 4594
SbfI CC_TGCA'GG
Cuts at: 4346 4346
Size: 6994
ScaI AGT'ACT
Cuts at: 5242 6954 5242
Size: 1712 5282
SfcI C'TryA_G
Cuts at: 826 1045 2568 3276 4342 5477 6158 6349 826
Size: 219 1523 708 1066 1135 681 191 1471
Fragments arranged by size:
1523 1471 1135 1066 708 681 219 191
SfoI GGC'GCC
Cuts at: 2056 2056
Size: 6994
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6994
SmaI CCC'GGG
Cuts at: 1872 2116 2134 4321 1872
Size: 244 18 2187 4545
Fragments arranged by size:
4545 2187 244 18
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5098 5999 6261 820
Size: 197 926 3155 901 262 1553
Fragments arranged by size:
3155 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4785 493
Size: 4292 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6994
SphI G_CATG'C
Cuts at: 1132 2335 3553 4352 1132
Size: 1203 1218 799 3774
Fragments arranged by size:
3774 1218 1203 799
SspI AAT'ATT
Cuts at: 5 52 4918 5
Size: 47 4866 2081
Fragments arranged by size:
4866 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6994
StyI C'CwwG_G
Cuts at: 513 1065 3216 3487 4433 513
Size: 552 2151 271 946 3074
Fragments arranged by size:
3074 2151 946 552 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2931 3365 3439 3897 5021 5038 5191
Size: 1255 1351 434 74 458 1124 17 153
Cuts at: 5191 5376 5821 325
Size: 185 445 1498
Fragments arranged by size:
1498 1351 1255 1124 458 445 434 185
153 74 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2799 2964 5240
Size: 274 80 33 51 1443 822 165 2276
Cuts at: 5240 6952 96
Size: 1712 138
Fragments arranged by size:
2276 1712 1443 822 274 165 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6954 6986 6954
Size: 32 6962
Tth111I GACn'n_nGTC
Cuts at: 1316 2192 3287 4156 1316
Size: 876 1095 869 4154
Fragments arranged by size:
4154 1095 876 869
XbaI T'CTAG_A
Cuts at: 4330 4330
Size: 6994
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3491 3854 3491
Size: 363 6631
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6994
XmaI C'CCGG_G
Cuts at: 1870 2114 2132 4319 1870
Size: 244 18 2187 4545
Fragments arranged by size:
4545 2187 244 18
XmnI GAAnn'nnTTC
Cuts at: 2430 5123 2430
Size: 2693 4301
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4802 276
Size: 53 83 186 936 3268 2468
Fragments arranged by size:
3268 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstZ17I EcoNI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SanDI
SapI SexAI SfiI SrfI SwaI